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https://www.readbyqxmd.com/read/29123535/trait-and-marker-associations-in-oryza-nivara-and-o-rufipogon-derived-rice-lines-under-two-different-heat-stress-conditions
#1
V Vishnu Prasanth, M Suchandranath Babu, Ramana K Basava, V G N Tripura Venkata, Satendra K Mangrauthia, S R Voleti, Sarla Neelamraju
Wild species and derived introgression lines (ILs) are a good source of genes for improving complex traits such as heat tolerance. The effect of heat stress on 18 yield traits was studied in four treatments in two seasons, under field conditions by subjecting 37 ILs and recurrent parents Swarna and KMR3, N22 mutants, and wild type and 2 improved rice cultivars to heat stress using polycover house method in wet season and late sowing method in dry season. Normal grown unstressed plants were controls. Both correlation and path coefficient analysis showed that the major contributing traits for high yield per plant (YPP) under heat stress conditions were tiller number, secondary branches in panicle, filled grain number, and percent spikelet fertility...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29116036/bifidobacterium-callitrichidarum-sp-nov-from-the-faeces-of-the-emperor-tamarin-saguinus-imperator
#2
Monica Modesto, Samanta Michelini, Maria Cristina Sansosti, Carlotta De Filippo, Duccio Cavalieri, Linnea Qvirist, Thomas Andlid, Caterina Spiezio, Camillo Sandri, Stefano Pascarelli, Barbara Sgorbati, Paola Mattarelli
Three Gram-stain-positive, non-spore-forming, microaerophilic and fructose-6-phosphate phosphoketolase positive strains were isolated from a faecal sample of an adult subject of the emperor tamarin (Saguinus imperator). Given that the isolates revealed identical BOX PCR profiles, strain TRI 5(T) was selected as a representative and characterized further. Comparative analysis of 16S rRNA gene sequence similarity revealed that strain TRI 5(T) was closely related to Bifidobacterium saguini DSM 23967(T) (96.4 %) and to Bifidobacterium longum subsp...
November 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29113809/mir21-drives-resistance-to-heat-shock-protein-90-inhibition-in-cholangiocarcinoma
#3
Andrea Lampis, Pietro Carotenuto, Georgios Vlachogiannis, Luciano Cascione, Somaieh Hedayat, Rosemary Burke, Paul Clarke, Else Bosma, Michele Simbolo, Aldo Scarpa, Sijia Yu, Rebecca Cole, Elizabeth Smyth, Javier Fernández Mateos, Ruwaida Begum, Blanka Hezelova, Zakaria Eltahir, Andrew Wotherspoon, Nicos Fotiadis, Maria Antonietta Bali, Chirag Nepal, Khurum Khan, Mark Stubbs, Jens C Hahne, Pierluigi Gasparini, Vincenza Guzzardo, Carlo M Croce, Suzanne Eccles, Matteo Fassan, David Cunningham, Jesper B Andersen, Paul Workman, Nicola Valeri, Chiara Braconi
BACKGROUND & AIMS: Cholangiocarcinomas (CCA) are resistant to chemotherapy, so new therapeutic agents are needed. We performed a screen to identify small molecule compounds that are active against CCAs. Levels of microRNA 21 (MIR21 or miRNA21) are increased in CCAs. We investigated whether miRNA21 mediates resistance of CCA cells and organoids to HSP90 inhibitors. METHODS: We performed a high-throughput screen of 484 small molecule compounds to identify those that reduced viability of 6 human CCA cell lines...
November 4, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29107750/molecular-cloning-prokaryotic-expression-purification-structural-studies-and-functional-implications-of-heat-shock-protein-70-hsp70-from-rutilus-frisii-kutum
#4
Zohreh Jahangirizadeh, Hossein Ghafouri, Reza H Sajedi, Sajjad Sarikhan, Majid Taghdir, Reyhaneh Sariri
A novel Hsp70 chaperone from Rutilus frisii kutum was identified, cloned, expressed, purified and its functional characteristics revealed. The 3D structure of Hsp70 from Rutilus kutum was constructed using the crystal structure of E. coli Hsp70 as the template, with 47% sequence identity. The in vitro ATPase activity assay after 60minutes, ATP hydrolysis of purified recombinant Hsp70 (8μM) was improved by binding to denatured thermally luciferase (3μM) about 2.5-fold compared with that of Hsp70 alone. Based on the results, it was found that the purified Hsp70 chaperone was able to considerably suppress heat-induced aggregation of luciferase by binding to DnaJ co-chaperone (5μM) more than 70% after 10min at 42°C...
October 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29097624/dnaj-homolog-subfamily-b-member-9-is-a-putative-autoantigen-in-fibrillary-gn
#5
Nicole K Andeen, Han-Yin Yang, Dao-Fu Dai, Michael J MacCoss, Kelly D Smith
Fibrillary GN is a rare form of GN of uncertain pathogenesis that is characterized by the glomerular accumulation of randomly arranged, nonbranching fibrils (12-24 nm) composed of Ig and complement proteins. In this study, we used mass spectrometry to comprehensively define the glomerular proteome in fibrillary GN compared with that in controls and nonfibrillary GN renal diseases. We isolated glomeruli from formalin-fixed and paraffin-embedded biopsy specimens using laser capture microdissection and analyzed them with liquid chromatography and data-dependent tandem mass spectrometry...
November 2, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29097623/dnaj-heat-shock-protein-family-b-member-9-is-a-novel-biomarker-for-fibrillary-gn
#6
Surendra Dasari, Mariam P Alexander, Julie A Vrana, Jason D Theis, John R Mills, Vivian Negron, Sanjeev Sethi, Angela Dispenzieri, W Edward Highsmith, Samih H Nasr, Paul J Kurtin
Fibrillary GN (FGN) is a rare primary glomerular disease. Histologic and histochemical features of FGN overlap with those of other glomerular diseases, and no unique histologic biomarkers for diagnosing FGN have been identified. We analyzed the proteomic content of glomeruli in patient biopsy specimens and detected DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9) as the fourth most abundant protein in FGN glomeruli. Compared with amyloidosis glomeruli, FGN glomeruli exhibited a >6-fold overexpression of DNAJB9 protein...
November 2, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29082339/dnajc18-a-novel-type-iii-dnaj-family-protein-is-expressed-specifically-in-rat-male-germ-cells
#7
Cynthia Gomes, Jaemog Soh
Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29079617/staphylococcus-edaphicus-sp-nov-isolated-in-antarctica-harbours-mecc-gene-and-genomic-islands-with-suspected-role-in-adaptation-to-extreme-environment
#8
Roman Pantůček, Ivo Sedláček, Adéla Indráková, Veronika Vrbovská, Ivana Mašlaňová, Vojtěch Kovařovic, Pavel Švec, Stanislava Králová, Lucie Krištofová, Jana Kekláková, Petr Petráš, Jiří Doškař
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus Staphylococcus that has near identical 16S rRNA gene sequence to Staphylococcus saprophyticus However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity <85 %, and inferred DNA-DNA hybridization <30%...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29063205/steered-molecular-dynamics-simulation-of-the-binding-of-the-bovine-auxilin-j-domain-to-the-hsc70-nucleotide-binding-domain
#9
You-Lin Xue, Lei Zhou, Yuna Sun, Hui Li, Gary W Jones, Youtao Song
The Hsp70 and Hsp40 chaperone machine plays critical roles in protein folding, membrane translocation, and protein degradation by binding and releasing protein substrates in a process that utilizes ATP. The activities of the Hsp70 family of chaperones are recruited and stimulated by the J domains of Hsp40 chaperones. However, structural information on the Hsp40-Hsp70 complex is lacking, and the molecular details of this interaction are yet to be elucidated. Here we used steered molecular dynamics (SMD) simulations to investigate the molecular interactions that occur during the dissociation of the auxilin J domain from the Hsc70 nucleotide-binding domain (NBD)...
October 23, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29018419/gene-expression-response-of-salmonella-enterica-serotype-enteritidis-phage-type-8-to-subinhibitory-concentrations-of-the-plant-derived-compounds-trans-cinnamaldehyde-and-eugenol
#10
Anup Kollanoor Johny, Jonathan G Frye, Annie Donoghue, Dan J Donoghue, Steffen Porwollik, Michael McClelland, Kumar Venkitanarayanan
Background:Salmonella Enteritidis phage type 8 (PT8) is a major poultry-associated Salmonella strain implicated in foodborne outbreaks in the United States. We previously reported that two plant-derived compounds generally recognized as safe (GRAS), trans-cinnamaldehyde (TC), and eugenol (EG), significantly reduced S. Enteritidis colonization in broiler and layer chickens. To elucidate potential PT8 genes affected by TC and EG during colonization, a whole-genome microarray analysis of the bacterium treated with TC and EG was conducted...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28964653/a-case-report-of-vibrio-furnissii-bacteremia-and-cellulitis-in-a-malnourished-patient-without-an-apparent-site-of-entry
#11
Takehiro Hashimoto, Saho Takaya, Satoshi Kutsuna, Kayoko Hayakawa, Daisuke Shiojiri, Yuichi Katanami, Kei Yamamoto, Nozomi Takeshita, Norio Ohmagari
We herein report a case of Vibrio furnissii bacteremia with bilateral lower limb cellulitis. A 53-year-old Japanese man with a mood disorder presented to our hospital with fever and a complaint of an inability to walk. Two sets of blood cultures became positive for V. furnissii. The treatment regimen was modified to ceftazidime and doxycycline. The patient recovered without relapse. Despite thorough examinations, portal of entry of V. furnissii remained unclear. Although the bacteria was first misidentified as V...
September 27, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28956354/heterologous-prime-boost-immunization-with-live-spy1-and-dnaj-protein-of-streptococcus-pneumoniae-induces-strong-th1-and-th17-cellular-immune-responses-in-mice
#12
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
Streptococcus pneumoniae is a leading cause of infectious diseases in children under 5-year-old. Vaccine has been used as an indispensable strategy to prevent S. pneumoniae infection for more than 30 years. Our previous studies confirmed that mucosal immunization with live attenuated strain SPY1 can protect mice against nasopharyngeal colonization of S. pneumoniae and lethal pneumococcal infection, and the protective effects are comparable with those induced by commercially available 23-valent polysaccharide vaccine...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28951887/proteomes-of-lactobacillus-delbrueckii-subsp-bulgaricus-lbb-b5-incubated-in-milk-at-optimal-and-low-temperatures
#13
Xiaochen Yin, Michelle R Salemi, Brett S Phinney, Velitchka Gotcheva, Angel Angelov, Maria L Marco
We identified the proteins synthesized by Lactobacillus delbrueckii subsp. bulgaricus strain LBB.B5 in laboratory culture medium (MRS) at 37°C and milk at 37 and 4°C. Cell-associated proteins were measured by gel-free, shotgun proteomics using high-performance liquid chromatography coupled with tandem mass spectrophotometry. A total of 635 proteins were recovered from all cultures, among which 72 proteins were milk associated (unique or significantly more abundant in milk). LBB.B5 responded to milk by increasing the production of proteins required for purine biosynthesis, carbohydrate metabolism (LacZ and ManM), energy metabolism (TpiA, PgK, Eno, SdhA, and GapN), amino acid synthesis (MetE, CysK, LBU0412, and AspC) and transport (GlnM and GlnP), and stress response (Trx, MsrA, MecA, and SmpB)...
September 2017: MSystems
https://www.readbyqxmd.com/read/28923495/crispr-cas9-engineering-of-adult-mouse-liver-demonstrates-that-the-dnajb1-prkaca-gene-fusion-is-sufficient-to-induce-tumors-resembling-fibrolamellar-hepatocellular-carcinoma
#14
Lars H Engelholm, Anjum Riaz, Denise Serra, Frederik Dagnæs-Hansen, Jens V Johansen, Eric Santoni-Rugiu, Steen H Hansen, Francesco Niola, Morten Frödin
BACKGROUND & AIMS: Fibrolamellar hepatocellular carcinoma (FL-HCC) is a primary liver cancer that predominantly affects young adults with no underlying liver disease. A somatic, 400 Kb deletion on chromosome 19 that fuses part of the DnaJ heat shock protein family (Hsp40) member B1 gene (DNAJB1) to the protein kinase cAMP-activated catalytic subunit alpha gene (PRKACA) has been repeatedly identified in patients with FL-HCC. However, the DNAJB1-PRKACA gene fusion has not been shown to induce liver tumorigenesis...
September 18, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28893355/bifidobacterium-vansinderenii-sp-nov-isolated-from-faeces-of-emperor-tamarin-saguinus-imperator
#15
Sabrina Duranti, Marta Mangifesta, Gabriele Andrea Lugli, Francesca Turroni, Rosaria Anzalone, Christian Milani, Leonardo Mancabelli, Maria Cristina Ossiprandi, Marco Ventura
A novel Bifidobacterium strain, Tam10B(T), i.e. LMG 30126(T), was isolated from emperor tamarin (Saguinus imperator). Cells were Gram-positive, non-motile, non-sporulating, non-haemolytic, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA genes as well as multilocus sequences (representing hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that Bifidobacterium Tam10B(T) exhibited close phylogenetic relatedness to Bifidobacterium tissieri DSM 100201(T)...
October 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28831037/the-molecular-chaperones-dnajb6-and-hsp70-cooperate-to-suppress-%C3%AE-synuclein-aggregation
#16
Francesco A Aprile, Emma Källstig, Galina Limorenko, Michele Vendruscolo, David Ron, Christian Hansen
A major hallmark of Parkinson's disease (PD) is the presence of Lewy bodies (LBs) in certain neuronal tissues. LBs are protein-rich inclusions, in which α-synuclein (α-syn) is the most abundant protein. Since these inclusions are not present in healthy individuals, despite the high concentration of α-syn in neurons, it is important to investigate whether natural control mechanisms are present to efficiently suppress α-syn aggregation. Here, we demonstrate that a CRISPR/Cas9-mediated knockout (KO) of a DnaJ protein, DNAJB6, in HEK293T cells expressing α-syn, causes a massive increase in α-syn aggregation...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819167/artificial-dnaj-protein-for-protein-production-and-conformational-diseases
#17
Akinori Hishiya, Keizo Koya
For secreted proteins, proper protein folding is essential not only for biological function but also for secretion itself. Proteins with folding problems are trapped in the endoplasmic reticulum (ER) and are eventually degraded in the cytoplasm. In this study, we exploited co-expression of an artificial fusion protein, based on the sequence of a DnaJ protein, which could interact as co-chaperones in the Hsp70-based protein-folding system, with target recombinant secreted proteins to enhance their production and secretion...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28769900/intrinsic-and-extrinsic-aspects-on-campylobacter-jejuni-biofilms
#18
Roberta T Melo, Eliane P Mendonça, Guilherme P Monteiro, Mariana C Siqueira, Clara B Pereira, Phelipe A B M Peres, Heriberto Fernandez, Daise A Rossi
Biofilm represents a way of life that allows greater survival of microorganisms in hostile habitats. Campylobacter jejuni is able to form biofilms in vitro and on surfaces at several points in the poultry production chain. Genetic determinants related to their formation are expressed differently between strains and external conditions are decisive in this respect. Our approach combines phylogenetic analysis and the presence of seven specific genes linked to biofilm formation in association with traditional microbiology techniques, using Mueller Hinton and chicken juice as substrates in order to quantify, classify, determine the composition and morphology of the biomass of simple and mixed biofilms of 30 C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28742844/control-of-runx-induced-repression-of-notch-signaling-by-mlf-and-its-partner-dnaj-1-during-drosophila-hematopoiesis
#19
Marion Miller, Aichun Chen, Vanessa Gobert, Benoit Augé, Mathilde Beau, Odile Burlet-Schiltz, Marc Haenlin, Lucas Waltzer
A tight regulation of transcription factor activity is critical for proper development. For instance, modifications of RUNX transcription factors dosage are associated with several diseases, including hematopoietic malignancies. In Drosophila, Myeloid Leukemia Factor (MLF) has been shown to control blood cell development by stabilizing the RUNX transcription factor Lozenge (Lz). However, the mechanism of action of this conserved family of proteins involved in leukemia remains largely unknown. Here we further characterized MLF's mode of action in Drosophila blood cells using proteomic, transcriptomic and genetic approaches...
July 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28714950/tid1-s-regulates-the-mitochondrial-localization-of-egfr-in-non-small-cell-lung-carcinoma
#20
T-H Wang, Y-H Lin, S-C Yang, P-C Chang, T-Cv Wang, C-Y Chen
The epidermal growth factor receptor (EGFR) is the major driver of non-small cell lung carcinoma (NSCLC). Mitochondrial accumulation of EGFR has been shown to promote metastasis in NSCLC, yet little is known about how the mitochondrial localization of EGFR is regulated. In this work, we show that Tid1 (also known as mitochondrial HSP40) is involved in the mitochondrial localization of EGFR, and that the DnaJ domain of Tid1-S is essential for the Tid1-S-mediated transportation of EGFR into mitochondria. Overexpression of Tid1-S increased the migration and invasion of NSCLC cells cultured in vitro...
July 17, 2017: Oncogenesis
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