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MTS peptide

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https://www.readbyqxmd.com/read/27878236/pyropia-yezoensis-peptide-promotes-collagen-synthesis-by-activating-the-tgf-%C3%AE-smad-signaling-pathway-in-the-human-dermal-fibroblast-cell-line-hs27
#1
Cho-Rong Kim, Young-Min Kim, Min-Kyeong Lee, In-Hye Kim, Youn-Hee Choi, Taek-Jeong Nam
Pyropia yezoensis (P. yezoensis) is a marine algae that exhibits antioxidant, anti-inflammatory, antitumor and anti-aging activities. In this study, we investigated the effects of the P. yezoensis peptide, PYP1‑5, on collagen synthesis in the human dermal fibroblast cell line Hs27. Skin aging is related to reduced collagen production and the activities of multiple enzymes, including matrix metalloproteinases (MMPs), which degrade collagen structure in the dermis, and tissue inhibitor of tissue inhibitor of metalloproteinases (TIMPs), which inhibit the action of MMPs...
November 18, 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27809498/theranostic-nanocages-for-imaging-and-photothermal-therapy-of-prostate-cancer-cells-by-active-targeting-of-neuropeptide-y-receptor
#2
Svetlana Avvakumova, Elisabetta Galbiati, Laura Sironi, Silvia A Locarno, Luca Gambini, Chiara Macchi, Laura Pandolfi, Massimiliano Ruscica, Paolo Magni, Maddalena Collini, Miriam Colombo, Fabio Corsi, Giuseppe Chirico, Sergio Romeo, Davide Prosperi
Gold nanocages (AuNCs) have been shown to be a useful tool for harnessing imaging and hyperthermia therapy of cancer, thanks to their unique optical properties, low toxicity and facile surface functionalization. Herein, we use AuNCs for selective targeting of prostate cancer cells (PC3) via specific interaction between neuropeptide Y (NPY) receptor and three different NPY analogs conjugated to AuNCs. Localized surface plasmon band of the nanoconjugates was set around 800 nm, which is appropriate for in vivo applications...
November 3, 2016: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27687527/evaluation-of-the-brain-penetrant-microtubule-stabilizing-agent-dictyostatin-in-the-ps19-tau-transgenic-mouse-model-of-tauopathy
#3
Vishruti Makani, Bin Zhang, Heeoon Han, Yuemang Yao, Pierrik Lassalas, Kevin Lou, Ian Paterson, Virginia M Y Lee, John Q Trojanowski, Carlo Ballatore, Amos B Smith, Kurt R Brunden
Neurodegenerative disorders referred to as tauopathies, which includes Alzheimer's disease (AD), are characterized by insoluble deposits of the tau protein within neuron cell bodies and dendritic processes in the brain. Tau is normally associated with microtubules (MTs) in axons, where it provides MT stabilization and may modulate axonal transport. However, tau becomes hyperphosphorylated and dissociates from MTs in tauopathies, with evidence of reduced MT stability and defective axonal transport. This has led to the hypothesis that MT-stabilizing drugs may have potential for the treatment of tauopathies...
September 29, 2016: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/27611949/f5-peptide-induces-aspermatogenesis-by-disrupting-organization-of-actin-and-microtubule-based-cytoskeletons-in-the-testis
#4
Ying Gao, Dolores D Mruk, Wing-Yee Lui, Will M Lee, C Yan Cheng
During the release of sperm at spermiation, a biologically active F5-peptide, which can disrupt the Sertoli cell tight junction (TJ) permeability barrier, is produced at the site of the degenerating apical ES (ectoplasmic specialization). This peptide coordinates the events of spermiation and blood-testis barrier (BTB) remodeling at stage VIII of the epithelial cycle, creating a local apical ES-BTB axis to coordinate cellular events across the epithelium. The mechanism(s) by which F5-peptide perturbs BTB restructuring, and its involvement in apical ES dynamics remain unknown...
September 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27608845/sialylation-transmogrifies-human-breast-and-pancreatic-cancer-cells-into-3d-multicellular-tumor-spheroids-using-cyclic-rgd-peptide-induced-self-assembly
#5
Roman Akasov, Sabah Haq, Fiona Haxho, Vanessa Samuel, Sergey V Burov, Elena Markvicheva, Ronald J Neufeld, Myron R Szewczuk
Multicellular tumor spheroids (MTS) have been at the forefront of cancer research, designed to mimic tumor-like developmental patterns in vitro. Tumor growth in vivo is highly influenced by aberrant cell surface-specific sialoglycan structures on glycoproteins. Aberrant sialoglycan patterns that facilitate MTS formation are not well defined. Matrix-free spheroids from breast MCF-7 and pancreatic PANC1 cancer cell lines and their respective tamoxifen (TMX) and gemcitabine (Gem) resistant variants were generated using the RGD platform of cyclic Arg-Gly-Asp-D-Phe-Lys peptide modified with 4-carboxybutyl-triphenylphosphonium bromide (cyclo-RGDfK (TPP))...
September 6, 2016: Oncotarget
https://www.readbyqxmd.com/read/27504265/towards-the-development-of-an-enzyme-replacement-therapy-for-the-metabolic-disorder-propionic-acidemia
#6
Mahnaz Darvish-Damavandi, Han Kiat Ho, Tse Siang Kang
Propionic acidemia (PA) is a life-threatening disease caused by the deficiency of a mitochondrial biotin-dependent enzyme known as propionyl coenzyme-A carboxylase (PCC). This enzyme is responsible for degrading the metabolic intermediate, propionyl coenzyme-A (PP-CoA), derived from multiple metabolic pathways. Currently, except for drastic surgical and dietary intervention that can only provide partial symptomatic relief, no other form of therapeutic option is available for this genetic disorder. Here, we examine a novel approach in protein delivery by specifically targeting and localizing our protein candidate of interest into the mitochondrial matrix of the cells...
September 2016: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/27485601/identification-of-the-minimum-pharmacophore-of-lipid-phosphatidylserine-ps-binding-peptide-peptoid-hybrid-pps1d1
#7
Jaspal Singh, Satya Prakash Shukla, Tanvi J Desai, D Gomika Udugamasooriya
We previously reported a unique peptide-peptoid hybrid, PPS1 that specifically recognizes lipid-phosphatidylserine (PS) and a few other negatively charged phospholipids, but not neutral phospholipids, on the cell membrane. The dimeric version of PPS1, i.e., PPS1D1 triggers strong cancer cell cytotoxicity and has been validated in lung cancer models both in vitro and in vivo. Given that PS and other negatively charged phospholipids are abundant in almost all tumor microenvironments, PPS1D1 is an attractive drug lead that can be developed into a globally applicable anti-cancer agent...
September 15, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27385409/polypeptides-from-the-skin-of-rana-chensinensis-exert-the-antioxidant-and-antiapoptotic-activities-on-hacat-cells
#8
Xin Zhang, Yunyun Cheng, Yang Yang, Songcai Liu, Hui Shi, Chao Lu, Siming Li, Linyan Nie, Dan Su, Xuming Deng, Kexiang Ding, Linlin Hao
Studies have shown that frog skin secretes many types of peptides that are good for human skin. In this study, acid and enzymatic extracts of Rana skin peptides (acid/enzymatic Rana skin peptides, ARPs/ERPs) were obtained. The chemical and physical properties of the ARPs and ERPs were identified through UV scanning, HGLC, FRIT, and MS. MTS and flow cytometry were used to test the proproliferative and antiapoptotic effects of the ARPs and ERPs on human immortalized keratinocytes (HaCaT cells). To elucidate the antiapoptotic mechanisms, the mRNA and protein levels of EGF (epidermal growth factor, which enhances stimulation of cellular proliferation in both cells and epithelial tissues) and caspase-3 were evaluated using quantitative RT-PCR...
July 6, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27291054/isolation-of-functional-tubulin-dimers-and-of-tubulin-associated-proteins-from-mammalian-cells
#9
Nuo Yu, Luca Signorile, Sreya Basu, Sophie Ottema, Joyce H G Lebbink, Kris Leslie, Ihor Smal, Dick Dekkers, Jeroen Demmers, Niels Galjart
The microtubule (MT) cytoskeleton forms a dynamic filamentous network that is essential for many processes, including mitosis, cell polarity and shape, neurite outgrowth and migration, and ciliogenesis [1, 2]. MTs are built up of α/β-tubulin heterodimers, and their dynamic behavior is in part regulated by tubulin-associated proteins (TAPs). Here we describe a novel system to study mammalian tubulins and TAPs. We co-expressed equimolar amounts of triple-tagged α-tubulin and β-tubulin using a 2A "self-cleaving" peptide and isolated functional fluorescent tubulin dimers from transfected HEK293T cells with a rapid two-step approach...
July 11, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27236098/investigation-of-human-cell-response-to-covalently-attached-rada16-i-peptide-on-silicon-surfaces
#10
Fahimeh Shamsi
We described a modification of the ionic (RADARADARADARADA)(1) peptide or RADA16-I with 4-azidophenyl isothiocyanate via a specific and gentle reaction. The azidated peptide was covalently immobilized on an alkyne-terminated monolayer on Si(111) via the Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition reaction. Detailed characterization using Impedance spectroscopy (IS), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy demonstrated high coverage of the RADA 16-I peptide on silicon surfaces...
September 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27107900/formation-of-multicellular-tumor-spheroids-induced-by-cyclic-rgd-peptides-and-use-for-anticancer-drug-testing-in-vitro
#11
Roman Akasov, Daria Zaytseva-Zotova, Sergey Burov, Maria Leko, Monique Dontenwill, Manuela Chiper, Thierry Vandamme, Elena Markvicheva
Development of novel anticancer formulations is a priority challenge in biomedicine. However, in vitro models based on monolayer cultures (2D) which are currently used for cytotoxicity tests leave much to be desired. More and more attention is focusing on 3D in vitro systems which can better mimic solid tumors. The aim of the study was to develop a novel one-step highly reproducible technique for multicellular tumor spheroid (MTS) formation using synthetic cyclic RGD-peptides, and to demonstrate availability of the spheroids as 3D in vitro model for antitumor drug testing...
June 15, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27093838/-expression-purification-of-recombinant-cationic-peptide-aik-in-escherichia-coli-and-its-antitumor-activity
#12
Fangfang Fan, Huiying Sun, Hui Xu, Jiawei Liu, Haiyuan Zhang, Yilan Li, Xuelian Ning, Yue Sun, Jing Bai, Songbin Fu, Chunshui Zhou
AIK is a novel cationic peptide with potential antitumor activity. In order to construct the AIK expression vector by Gateway technology, and establish an optimal expression and purification method for recombinant AIK, a set of primers containing AttB sites were designed and used to create the AttB-TEV-FLAG-AIR fusion gene by overlapping PCR. The resulting fusion gene was cloned into the donor vector pDONR223 by attB and attP mediated recombination (BP reaction), then, transferred into the destination vector pDESTl 5 by attL and attR mediated recombination (LR reaction)...
December 2015: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/27061576/oxidation-of-the-n-terminal-domain-of-the-wheat-metallothionein-ec-1-leads-to-the-formation-of-three-distinct-disulfide-bridges
#13
Katsiaryna Tarasava, Serge Chesnov, Eva Freisinger
Metallothioneins (MTs) are low molecular weight proteins, characterized by a high cysteine content and the ability to coordinate large amounts of d(10) metal ions, for example, Zn(II), Cd(II), and Cu(I), in form of metal-thiolate clusters. Depending on intracellular conditions such as redox potential or metal ion concentrations, MTs can occur in various states ranging from the fully metal-loaded holo- to the metal-free apo-form. The Cys thiolate groups in the apo-form can be either reduced or be involved in disulfide bridges...
May 2016: Biopolymers
https://www.readbyqxmd.com/read/27052575/rutaecarpine-attenuates-hypoxia-induced-right-ventricular-remodeling-in-rats
#14
Wen-Qun Li, Xiao-Hui Li, Jie Du, Wang Zhang, Dai Li, Xiao-Ming Xiong, Yuan-Jian Li
Rutaecarpine has been shown to exhibit wide pharmacological effects in the cardiovascular system via stimulation of calcitonin gene-related peptide (CGRP) release. In the present study, the effect of rutaecarpine on hypoxia-induced right ventricular (RV) remodeling and the underlying mechanisms were evaluated. RV remodeling was induced by hypoxia (10 % O2, 3 weeks) in rats. Rats were treated with rutaecarpine (20 or 40 mg/kg) by intragastric administration. Proliferation of cardiac fibroblasts was induced by TGF-β1 (5 ng/mL) and determined by MTS and EdU incorporation method...
July 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/27043257/effects-of-c-terminal-amelogenin-peptide-on-proliferation-of-human-cementoblast-lineage-cells
#15
Yuki Yoshimi, Ryo Kunimatsu, Naoto Hirose, Tetsuya Awada, Mutsumi Miyauchi, Takashi Takata, Wu Li, Li Zhu, Pamela Denbesten, Kazuo Tanne, Kotaro Tanimoto
BACKGROUND: Extracts of enamel matrix proteins are used to regenerate periodontal tissue; amelogenin, the most abundant enamel protein, plays an important role in this regeneration. Studies have demonstrated that amelogenin fragments promote tissue regeneration, but the bioactive site of amelogenin remains unclear. This study explores the functional domain of amelogenin by investigating effects of four amelogenin species on cementoblast proliferation. METHODS: Four amelogenin species based on amelogenin cleavage products were investigated: 1) recombinant human full-length amelogenin (rh174); 2) amelogenin cleavage product lacking the C-terminal (rh163); 3) amelogenin cleavage product lacking the N-terminal (rh128); and 4) the C-terminal region of rh174 (C11 peptide), which was synthesized and purified...
July 2016: Journal of Periodontology
https://www.readbyqxmd.com/read/26862583/comparison-of-bactericidal-and-cytotoxic-activities-of-trichogin-analogs
#16
Regina Tavano, Giulia Malachin, Marta De Zotti, Cristina Peggion, Barbara Biondi, Fernando Formaggio, Emanuele Papini
Peptaibiotics are a group of membrane active peptides of fungal origin. They typically contain α-aminoisobutyric acid (Aib; 1-letter code, U) and other non-coded residues (Toniolo and Brückner, 2009; Neumann et al., 2015; Benedett et al., 1982) [1], [2], [3] stabilizing their helical structure. Peptaibols are peptaibiotics carrying a 1, 2-aminoalcohol at the C-terminus. When a fatty acid chain (of 8-10 carbon atoms) is present at their N-terminus, they are called lipopeptaibols (Toniolo et al., 2001; Degenkolb et al...
March 2016: Data in Brief
https://www.readbyqxmd.com/read/26675044/a-continuous-kinetic-assay-for-protein-and-dna-methyltransferase-enzymatic-activities
#17
Shai Duchin, Zlata Vershinin, Dan Levy, Amir Aharoni
BACKGROUND: Methyltransferases (MTs) catalyze the S-adenosylmethionine (SAM)-dependent methylation of a wide variety of protein and DNA substrates. Methylation of lysine, arginine or cytosine regulates a variety of biological processes including transcriptional activation and gene silencing. Despite extensive studies of the cellular roles of MTs, their quantitative kinetic characterization remains challenging. In the past decade, several assays have been developed to monitor methyl transfer activity utilizing different approaches including radiolabeling, antibodies or mass-spectrometry analysis...
2015: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/26626627/metal-induction-of-a-pisolithus-albus-metallothionein-and-its-potential-involvement-in-heavy-metal-tolerance-during-mycorrhizal-symbiosis
#18
M Sudhakara Reddy, Manpreet Kour, Sipla Aggarwal, Shanky Ahuja, Roland Marmeisse, Laurence Fraissinet-Tachet
Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd...
September 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/26574392/long-time-step-molecular-dynamics-through-hydrogen-mass-repartitioning
#19
Chad W Hopkins, Scott Le Grand, Ross C Walker, Adrian E Roitberg
Previous studies have shown that the method of hydrogen mass repartitioning (HMR) is a potentially useful tool for accelerating molecular dynamics (MD) simulations. By repartitioning the mass of heavy atoms into the bonded hydrogen atoms, it is possible to slow the highest-frequency motions of the macromolecule under study, thus allowing the time step of the simulation to be increased by up to a factor of 2. In this communication, we investigate further how this mass repartitioning allows the simulation time step to be increased in a stable fashion without significantly increasing discretization error...
April 14, 2015: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/26548930/mitochondria-targeted-drug-delivery-system-for-cancer-treatment
#20
Zhi-Peng Chen, Man Li, Liu-Jie Zhang, Jia-Yu He, Li Wu, Yan-Yu Xiao, Jin-Ao Duan, Ting Cai, Wei-Dong Li
Mitochondria are one type of the major organelles in the cell, participating in a variety of important physiological and biochemical processes, such as tricarboxylic acid cycle, fatty acid metabolism and oxidative phosphorylation. Meanwhile, it also happens to be the key regulator of apoptosis by triggering the complex cell-death processes through a variety of mechanisms. Since it plays a pivotal role in cell-death, a mitochondria-targeted treatment strategy could be promising for cancer therapy. In this comprehensive review, we focused on the mechanisms of mitochondrial targeting and a variety of strategies to realize the purpose of mitochondrial targeting, including that based on the use of lipophilic cations, and mitochondrial targeting signal peptides (MTS) as well as cell-penetrating peptides (CPPs)...
2016: Journal of Drug Targeting
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