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MTS peptide

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https://www.readbyqxmd.com/read/29158259/structural-analyses-of-key-features-in-the-kank1-kif21a-complex-yield-mechanistic-insights-into-the-crosstalk-between-microtubules-and-the-cell-cortex
#1
Zhuangfeng Weng, Yuan Shang, Deqiang Yao, Jinwei Zhu, Rongguang Zhang
The crosstalk between dynamic microtubules (MTs) and the cell cortex plays important roles in cell division, polarity, and migration. A critical adaptor that links the plus ends of MTs with the cell cortex is the KN motif and ankyrin repeat domains 1 (KANK1)/kinesin family member 21A (KIF21A) complex. Genetic defects in these two proteins are associated with various cancers and developmental diseases such as congenital fibrosis of the extraocular muscles type 1 (CFEOM1). However, the molecular mechanism governing the KANK1/KIF21A interaction and the role of the conserved ankyrin (ANK) repeats in this interaction are still unclear...
November 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29114657/chemistry-of-mammalian-metallothioneins-and-their-interaction-with-amyloidogenic-peptides-and-proteins
#2
Elena Atrián-Blasco, Alice Santoro, Dean L Pountney, Gabriele Meloni, Christelle Hureau, Peter Faller
Cu and Zn ions are essential in most living beings. Their metabolism is critical for health and mis-metabolism can be lethal. In the last two decades, a large body of evidence has reported the role of copper, zinc and iron, and oxidative stress in several neurodegenerative diseases like Alzheimer's, Parkinson's, prion diseases, etc. To what extent this mis-metabolism is causative or a consequence of these diseases is still a matter of research. In this context metallothioneins (MTs) appear to play a central gate-keeper role in controlling aberrant metal-protein interactions...
November 8, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/29043393/characterization-of-a-set-of-abdominal-neuroendocrine-cells-that-regulate-stress-physiology-using-colocalized-diuretic-peptides-in-drosophila
#3
Meet Zandawala, Richard Marley, Shireen A Davies, Dick R Nässel
Multiple neuropeptides are known to regulate water and ion balance in Drosophila melanogaster. Several of these peptides also have other functions in physiology and behavior. Examples are corticotropin-releasing factor-like diuretic hormone (diuretic hormone 44; DH44) and leucokinin (LK), both of which induce fluid secretion by Malpighian tubules (MTs), but also regulate stress responses, feeding, circadian activity and other behaviors. Here, we investigated the functional relations between the LK and DH44 signaling systems...
October 17, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28929935/development-of-arginine-glycine-aspartate-immobilized-3d-printed-poly-propylene-fumarate-scaffolds-for-cartilage-tissue-engineering
#4
Chi Bum Ahn, Youngjo Kim, Sung Jean Park, Yongsung Hwang, Jin Woo Lee
Poly(propylene fumarate) (PPF) has known to be a good candidate material for cartilage tissue regeneration because of its excellent mechanical properties during its degradation processes. Here, we describe the potential application of PPF-based materials as 3D printing bioinks to create macroporous cell scaffolds using micro-stereolithography (MSTL). To improve cell-matrix interaction of seeded human chondrocytes within the PPF-based 3D scaffolds, we immobilized arginine-glycine-aspartate (RGD) peptide onto the PPF scaffolds...
September 20, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28927682/mgf-e-peptide-pretreatment-improves-the-proliferation-and-osteogenic-differentiation-of-bmscs-via-mek-erk1-2-and-pi3k-akt-pathway-under-severe-hypoxia
#5
Yongqiang Sha, Yonggang Lv, Zhiling Xu, Li Yang, Xiaoying Hao, Ruli Afandi
AIMS: Severe hypoxia always inhibits the cell proliferation, osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs), and hinders bone defect repair. Herein we explored the effects of mechano-growth factor (MGF) E peptide on the proliferation and osteogenic differentiation of BMSCs under severe hypoxia. MATERIALS AND METHODS: CoCl2 was utilized to simulate severe hypoxia. MTS was used to detect cell viability. Cell proliferation was verified through flow cytometry and EdU assay...
November 15, 2017: Life Sciences
https://www.readbyqxmd.com/read/28899926/peptides-mediating-dna-transport-on-microtubules-and-their-impact-on-non-viral-gene-transfer-efficiency
#6
REVIEW
Patrick Midoux, Lucie Pigeon, Cristine Gonçalves, Chantal Pichon
Synthetic vectors such as cationic polymers and cationic lipids remain attractive tools for non-viral gene transfer which is a complex process whose effectiveness relies on the ability to deliver a plasmid DNA (pDNA) into the nucleus of non-dividing cells. Once in the cytosol, the transport of pDNAs towards the nuclear envelope is strongly impaired by their very low cytosolic mobility due to their large size. To promote their movement towards the cell nucleus, few strategies have been implemented to exploit dynein, the microtubule's (MT's) motor protein, for propagation of cytosolic pDNA along the MTs towards the cell nucleus...
October 31, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28861789/neurotoxicity-of-prion-peptides-on-cultured-cerebellar-neurons
#7
Giuseppe D Ciccotosto, Metta Jana, Roberto Cappai
Prion neurotoxicity has been modeled in vitro using synthetic peptides derived from the PrP(C) sequence. The major region of neurotoxicity has been localized to the hydrophobic domain located in the middle of the PrP protein. Neurotoxicity assays are typically performed on cultured mouse cerebellar neurons derived from neonatal pups, and cell viability can be monitored by assays including MTT or MTS, cell death by LDH release, or apoptosis by caspase cleavage assays. These neurotoxicity studies have been useful in identifying cofactors, such as PrP(C) and metals, as modulators of PrP peptide-mediated neurotoxicity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28797801/dimeric-and-trimeric-homo-and-heteroleptic-hydroxamic-acid-macrocycles-formed-using-mixed-ligand-fe-iii-based-metal-templated-synthesis
#8
Athavan Sresutharsan, William Tieu, Tomas Richardson-Sanchez, Cho Zin Soe, Rachel Codd
Macrocyclic hydroxamic acids coordinate Fe(III) with high affinity as part of siderophore-mediated bacterial iron acquisition. Trimeric hydroxamic acid macrocycles, such as desferrioxamine E (DFOE), are prevalent in nature, with fewer dimeric macrocycles identified, including putrebactin (pbH2), avaroferrin (avH2), bisucaberin (bsH2) and alcaligin (alH2). This work used metal-templated synthesis (MTS) to pre-assemble complexes between one equivalent of Fe(III) and two equivalents of 4-((4-aminobutyl)(hydroxy)amino)-4-oxobutanoic acid (BBH) or 4-((5-aminopentyl)(hydroxy)amino)-4-oxobutanoic acid (PBH)...
August 1, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28708281/cooperation-of-hif-and-ncam-mediated-mechanisms-in-cell-viability-of-hippocampal-cultures-after-oxygen-glucose-deprivation
#9
Iryna Lushnikova, Yelyzaveta Nikandrova, Galyna Skibo
Neurodegenerative diseases of different genesis are the result of cellular damages including those caused by oxygen and glucose deficit. Neuronal survival or death in brain pathologies depends on a variety of interrelated molecular mechanisms. A key role in modulation of neuron viability belongs to HIF (hypoxia-inducible factor) and NCAM (neural cell adhesion molecules) signaling pathways. In this work, we used organotypic and dissociated hippocampal cultures to analyze cell viability and HIF-1α immunopositive (HIF-1α(+) ) signal after 30 min oxygen-glucose deprivation (OGD) followed by 24 h of reoxygenation in the presence of FGL (synthetic NCAM-derived mimetic peptide)...
July 14, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28550801/the-nk-1-receptor-antagonist-l-732-138-induces-apoptosis-in-human-gastrointestinal-cancer-cell-lines
#10
Miguel Muñoz, Marisa Rosso, Rafael Coveñas
BACKGROUND: Gastric and colon cancer cells express the neurokinin-1 receptor (NK-1R) and the peptide substance P (SP), after binding to this receptor, elicits the proliferation of gastrointestinal cancer cells and an antiapoptotic effect. In these cells, NK-1R antagonists (L-733,060: a piperidine derivative; aprepitant: a morpholine derivative) block, after binding to the NK-1R, the action of SP and exert an antiproliferative action, both antagonists promote apoptosis and the death of cancer cells...
February 12, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28393223/pyropia-yezoensis-peptide-pyp1%C3%A2-5-protects-against-dexamethasone%C3%A2-induced-muscle-atrophy-through-the-downregulation-of-atrogin1-mafbx-and-murf1-in-mouse-c2c12-myotubes
#11
Min-Kyeong Lee, Young-Min Kim, In-Hye Kim, Youn-Hee Choi, Taek-Jeong Nam
Skeletal muscle atrophy refers to the decline in muscle mass and strength that occurs under various conditions, including aging, starvation, cancer and other cachectic diseases. Muscle atrophy caused by aging, known as sarcopenia, primarily occurs after 50 years of age. Muscle atrophy‑related genes, including atrogin1/muscle atrophy F‑box (MAFbx) and muscle RING finger 1 (MuRF1), are expressed early in the muscle atrophy process, and their expression precedes the loss of muscle mass. The present study investigated the potential anti‑atrophic effects of the Pyropia yezoensis peptide PYP1‑5...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28245117/octadentate-zirconium-iv-loaded-macrocycles-with-varied-stoichiometry-assembled-from-hydroxamic-acid-monomers-using-metal-templated-synthesis
#12
William Tieu, Tulip Lifa, Andrew Katsifis, Rachel Codd
The reaction between Zr(IV) and the forward endo-hydroxamic acid monomer 4-[(5-aminopentyl)(hydroxy)amino]-4-oxobutanoic acid (for-PBH) in a 1:4 stoichiometry in the presence of diphenylphosphoryl azide and triethylamine gave the octadentate Zr(IV)-loaded tetrameric hydroxamic acid macrocycle for-[Zr(DFOT1)] ([M + H](+) calc 887.3, obs 887.2). In this metal-templated synthesis (MTS) approach, the coordination preferences of Zr(IV) directed the preorganization of four oxygen-rich bidentate for-PBH ligands about the metal ion prior to ring closure under peptide coupling conditions...
February 28, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28137574/quantitation-of-human-metallothionein-isoforms-in-cells-tissues-and-cerebrospinal-fluid-by-mass-spectrometry
#13
J B Shabb, W W Muhonen, A A Mehus
Metallothioneins (MTs) are a family of small, highly conserved, cysteine-rich metal-binding proteins that are important for zinc and copper homeostasis, protection against oxidative stress, and buffering against toxic heavy metals. Individual human MT isoforms are candidate biomarkers for heavy metal toxicity, and selected cancers and neurodegenerative diseases. The similar antigenicity of human MT-1 and MT-2 isoforms precludes development of antibody-based assays for their individual quantitation. Metal-based MT quantitation methods do not directly measure MT isoforms...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28115743/adnp-nap-dramatically-increase-microtubule-end-binding-protein-tau-interaction-a-novel-avenue-for-protection-against-tauopathy
#14
Y Ivashko-Pachima, C Laura Sayas, A Malishkevich, I Gozes
Activity-dependent neuroprotective protein (ADNP), vital for brain formation and cognitive function, is mutated in autism and linked to neurodegenerative/psychiatric diseases. An eight-amino-acid peptide snippet of ADNP, NAP (NAPVSIPQ), identified as a smallest active fragment, includes the SxIP microtubule (MT) end-binding protein (EB) association motif, and enhances ADNP-EB3 interaction. Depletion of EB1 or EB3 abolishes NAP protection against zinc intoxication. Furthermore, NAP enhances Tau-MT interaction, and Tau regulates the localization and function of EB1 and EB3 in developing neuronal cells...
September 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28088065/methionine-enkephalin-menk-mounts-antitumor-effect-via-regulating-dendritic-cells-dcs
#15
Yiming Meng, Xinghua Gao, Wenna Chen, Nicolas P Plotnikoff, Noreen Griffin, Guirong Zhang, Fengping Shan
MENK, an endogenous opioid peptide has been reported to have many immunological and antitumor activities. So far the detailed mechanisms of antitumor through regulating DCs by MENK have not been elucidated yet. The aim of this work was to investigate the antitumor mechanisms of MENK via regulating DC. The monitoring methods, such as ELISA, MTS assay, CFSE, Real-time PCR and Western blot were included in our research. We found bone marrow derived dendritic cells (BMDCs) in 36 female C57BL/6 mice treated with MENK enhanced expression of key surface molecules, increased production of critical cytokines reduced endocytosis of FITC-dextran, upregulated TLR4 through MyD88/NF-κB signaling pathway and mounted higher antitumor activity...
March 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/27919683/effects-of-c-terminal-amelogenin-peptides-on-the-metabolism-of-osteoblasts
#16
Tetsuya Awada, Ryo Kunimatsu, Yuki Yoshimi, Naoto Hirose, Tomomi Mitsuyoshi, Keisuke Sumi, Kotaro Tanimoto
OBJECTIVES: Amelogenins, enamel matrix proteins secreted by ameloblasts, comprise 90% of the developing extracellular enamel matrix. Recent evidence suggests that amelogenins might induce the proliferation of various cells. However, the residues comprising the active site of amelogenin remain unclear. Therefore, this study aimed to examine the effects of a human amelogenin C-terminal peptide (amgCP) on the metabolism of osteoblasts. MATERIALS AND METHODS: Mouse calvarial osteoblastic cells (MC3T3-E1) were cultured and treated with amgCP...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27878236/pyropia-yezoensis-peptide-promotes-collagen-synthesis-by-activating-the-tgf-%C3%AE-smad-signaling-pathway-in-the-human-dermal-fibroblast-cell-line-hs27
#17
Cho-Rong Kim, Young-Min Kim, Min-Kyeong Lee, In-Hye Kim, Youn-Hee Choi, Taek-Jeong Nam
Pyropia yezoensis (P. yezoensis) is a marine algae that exhibits antioxidant, anti-inflammatory, antitumor and anti-aging activities. In this study, we investigated the effects of the P. yezoensis peptide, PYP1‑5, on collagen synthesis in the human dermal fibroblast cell line Hs27. Skin aging is related to reduced collagen production and the activities of multiple enzymes, including matrix metalloproteinases (MMPs), which degrade collagen structure in the dermis, and tissue inhibitor of tissue inhibitor of metalloproteinases (TIMPs), which inhibit the action of MMPs...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27809498/theranostic-nanocages-for-imaging-and-photothermal-therapy-of-prostate-cancer-cells-by-active-targeting-of-neuropeptide-y-receptor
#18
Svetlana Avvakumova, Elisabetta Galbiati, Laura Sironi, Silvia A Locarno, Luca Gambini, Chiara Macchi, Laura Pandolfi, Massimiliano Ruscica, Paolo Magni, Maddalena Collini, Miriam Colombo, Fabio Corsi, Giuseppe Chirico, Sergio Romeo, Davide Prosperi
Gold nanocages (AuNCs) have been shown to be a useful tool for harnessing imaging and hyperthermia therapy of cancer, thanks to their unique optical properties, low toxicity, and facile surface functionalization. Herein, we use AuNCs for selective targeting of prostate cancer cells (PC3) via specific interaction between neuropeptide Y (NPY) receptor and three different NPY analogs conjugated to AuNCs. Localized surface plasmon resonance band of the nanoconjugates was set around 800 nm, which is appropriate for in vivo applications...
December 21, 2016: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27687527/evaluation-of-the-brain-penetrant-microtubule-stabilizing-agent-dictyostatin-in-the-ps19-tau-transgenic-mouse-model-of-tauopathy
#19
Vishruti Makani, Bin Zhang, Heeoon Han, Yuemang Yao, Pierrik Lassalas, Kevin Lou, Ian Paterson, Virginia M Y Lee, John Q Trojanowski, Carlo Ballatore, Amos B Smith, Kurt R Brunden
Neurodegenerative disorders referred to as tauopathies, which includes Alzheimer's disease (AD), are characterized by insoluble deposits of the tau protein within neuron cell bodies and dendritic processes in the brain. Tau is normally associated with microtubules (MTs) in axons, where it provides MT stabilization and may modulate axonal transport. However, tau becomes hyperphosphorylated and dissociates from MTs in tauopathies, with evidence of reduced MT stability and defective axonal transport. This has led to the hypothesis that MT-stabilizing drugs may have potential for the treatment of tauopathies...
September 29, 2016: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/27611949/f5-peptide-induces-aspermatogenesis-by-disrupting-organization-of-actin-and-microtubule-based-cytoskeletons-in-the-testis
#20
Ying Gao, Dolores D Mruk, Wing-Yee Lui, Will M Lee, C Yan Cheng
During the release of sperm at spermiation, a biologically active F5-peptide, which can disrupt the Sertoli cell tight junction (TJ) permeability barrier, is produced at the site of the degenerating apical ES (ectoplasmic specialization). This peptide coordinates the events of spermiation and blood-testis barrier (BTB) remodeling at stage VIII of the epithelial cycle, creating a local apical ES-BTB axis to coordinate cellular events across the epithelium. The mechanism(s) by which F5-peptide perturbs BTB restructuring, and its involvement in apical ES dynamics remain unknown...
September 27, 2016: Oncotarget
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