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Itch protein

Chung Eui You, Seok Hoon Moon, Kwang Hoon Lee, Kyu Han Kim, Chun Wook Park, Seong Joon Seo, Sang Hyun Cho
BACKGROUND: Atopic dermatitis (AD) is a common, complex disease that follows a chronic relapsing course and significantly affects the quality of life of patients. Skin barrier dysfunction and inflammatory processes induce and aggravate this skin condition. Proper use of an emollient for hydration is a keystone of AD treatment. Bee venom is known to have anti-inflammatory effects and has been widely used in traditional medicine to treat various inflammatory disorders. OBJECTIVE: To find out the beneficial effect of an emollient containing bee venom in the treatment of patients with AD...
October 2016: Annals of Dermatology
Young Bong Choi, Noula Shembade, Kislay Parvatiyar, Siddharth Balachandran, Edward William Harhaj
The host response to RNA virus infection consists of an intrinsic innate immune response and the induction of apoptosis as mechanisms to restrict viral replication. The mitochondrial adaptor molecule MAVS plays critical roles in coordinating both virus-induced type I interferon production and apoptosis; however, the regulation of MAVS-mediated apoptosis is poorly understood. Here, we show that the adaptor protein TAX1BP1 functions as a negative regulator of virus-induced apoptosis. TAX1BP1-deficient cells are highly sensitive to apoptosis in response to infection with the RNA viruses vesicular stomatitis virus and Sendai virus and transfection with poly(I:C)...
October 10, 2016: Molecular and Cellular Biology
Thomas Mettang, Kevin Kunzmann, Heinz-Jürgen Roth, Elke Weisshaar
is missing (Short communication).
September 27, 2016: Acta Dermato-venereologica
Steven Vanderschueren, Annelien van der Veen
OBJECTIVES: The Schnitzler syndrome is a rare inflammatory disorder, with a chronic urticaria-like rash and an IgM (rarely IgG) monoclonal gammopathy as cardinal features. Interleukin-1 β is regarded as the key mediator and the interleukin-1 receptor antagonist anakinra has been proposed as first-line treatment. This case series of eleven patients is intended to enhance disease awareness and to compare our centre's experience with that of literature. METHODS: We describe the clinical features and disease course of 11 patients with a definite Schnitzler syndrome, according to the Strasbourg diagnostic criteria, encountered in the University Hospital, Leuven, Belgium, between 1995 and 2015...
August 31, 2016: Clinical and Experimental Rheumatology
Shen Kiat Lim, Ssu Yi Lu, Shin-Ae Kang, Hock Jin Tan, Zilin Li, Zhen Ning Adrian Wee, Jye Swei Guan, Vishnu Priyanka Reddy Chichili, J Sivaraman, Thomas Putti, Aye Aye Thike, Puay Hoon Tan, Marius Sudol, David M Virshup, Siew Wee Chan, Wanjin Hong, Yoon Pin Lim
Crosstalk between the Hippo and Wnt signaling pathways has been implicated recently in breast cancer development, but key intersections have yet to be fully defined. Here we report that WBP2, a transcriptional coactivator that binds the Hippo pathway transcription factor YAP/TAZ, contributes to Wnt signaling and breast cancer pathogenesis. Clinically, overexpression of WBP2 in breast cancer specimens correlated with malignant progression and poor patient survival. In breast cancer cells, nuclear entry and interaction of WBP2 with beta-catenin was stimulated by Wnt3A, thereby activating TCF-mediated transcription and driving malignant invasive character...
August 30, 2016: Cancer Research
Shing-Hong Lin, Martin Steinhoff, Akihiko Ikoma, Yen-Ching Chang, Yuan-Ren Cheng, Ravi Chandra Kopparaju, Satoshi Ishii, Wei-Hsin Sun, Chih-Cheng Chen
Itch and pain are closely related but are distinct sensations. Intradermal injection of acid generates pain in both rodents and humans; however, few studies have addressed the intriguing question of whether acid (protons) can evoke itch like other algogens by spatial contrast activation of single nociceptors. Here, we report that (i) citric acid (0.2 mol/L) pH-dependently induced a scratching response in mice when applied intradermally to nape or cheek skin, (ii) acidified buffer elevated intracellular calcium levels in dorsal root ganglion pruriceptors, and (iii) injection of intradermal citric acid (pH 3...
August 24, 2016: Journal of Investigative Dermatology
Katrin Schaper, Kristine Rossbach, Brigitta Köther, Holger Stark, Manfred Kietzmann, Thomas Werfel, Ralf Gutzmer
The cytokine thymic stromal lymphopoietin (TSLP) is involved in the development and the progression of allergic diseases. It is mainly released by epithelial cells at barriers such as skin and gut in response to danger signals. Overexpression of TSLP in keratinocytes (KC) can provoke the development of a type 2 inflammatory response. Additionally, TSLP directly acts on sensory neurons and thereby triggers itch. Since histamine is also increased in lesions of inflammatory skin diseases, the aim of this study was to investigate possible effects of histamine as well as different histamine receptor subtype agonists and antagonists on TSLP production in KC...
August 1, 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Ziying Han, Cari A Sagum, Mark T Bedford, Sachdev S Sidhu, Marius Sudol, Ronald N Harty
UNLABELLED: Ebola virus (EBOV) and Marburg virus (MARV) belong to the Filoviridae family and can cause outbreaks of severe hemorrhagic fever, with high mortality rates in humans. The EBOV VP40 (eVP40) and MARV VP40 (mVP40) matrix proteins play a central role in virion assembly and egress, such that independent expression of VP40 leads to the production and egress of virus-like particles (VLPs) that accurately mimic the budding of infectious virus. Late (L) budding domains of eVP40 recruit host proteins (e...
October 15, 2016: Journal of Virology
Chung-Pei Lee, Guan-Ting Liu, Hsiu-Ni Kung, Po-Ting Liu, Yen-Tzu Liao, Lu-Ping Chow, Ling-Shih Chang, Yu-Hsin Chang, Chou-Wei Chang, Wen-Chi Shu, Annie Angers, Antonella Farina, Su-Fang Lin, Ching-Hwa Tsai, Fadila Bouamr, Mei-Ru Chen
UNLABELLED: The cellular endosomal sorting complex required for transport (ESCRT) was recently found to mediate important morphogenesis processes at the nuclear envelope (NE). We previously showed that the Epstein-Barr virus (EBV) BFRF1 protein recruits the ESCRT-associated protein Alix to modulate NE structure and promote EBV nuclear egress. Here, we uncover new cellular factors and mechanisms involved in this process. BFRF1-induced NE vesicles are similar to those observed following EBV reactivation...
October 15, 2016: Journal of Virology
Rongfeng Li, Huahua Yu, Yang Yue, Song Liu, Ronge Xing, Xiaolin Chen, Pengcheng Li
UNLABELLED: Jellyfish sting has become a worldwide issue of critical concern to human health and safety in coastal areas in recent decades. Cyanea nozakii is one of the dominant blooming species and dangerous stingers in China. However, it remains unclear how many and what types of toxins are present in the venom. So, we used a combined transcriptomics and proteomics approach to investigate the venom composition of jellyfish C. nozakii. In total 4,608,524 Illumina valid reads were obtained to de novo assemble to 40,434 unigenes in the transcriptomics analysis...
October 4, 2016: Journal of Proteomics
Feng Li, Miao Xu, Chao Qin, Lu Xia, Yanqing Xiong, Xiuhong Xi, Xiaoyong Fan, Jun Gu, Jiang Pu, Qi Wu, Shuihua Lu, Guozhi Wang
OBJECTIVE: To assess the accuracy and safety of the ESAT6-CFP10 reagent in diagnosing tuberculosis (TB) disease. METHODS: An open-label, randomised phase IIa trial was conducted in 56 healthy adults and 88 TB patients at one medical centre and one teaching hospital in China. All participants received 0.1, 0.5, 1 or 2 μg ESAT6-CFP10 in their right forearm. Moreover, 56 healthy volunteers and 56 patients were given tuberculin-purified protein derivative (TB-PPD) in their left forearm...
July 21, 2016: Clinical Microbiology and Infection
Hariharan Subramanian, Kshitij Gupta, Hydar Ali
Mast cells (MCs), which are granulated tissue-resident cells of hematopoietic lineage, contribute to vascular homeostasis, innate/adaptive immunity, and wound healing. However, MCs are best known for their roles in allergic and inflammatory diseases, such as anaphylaxis, food allergy, rhinitis, itch, urticaria, atopic dermatitis, and asthma. In addition to the high-affinity IgE receptor (FcεRI), MCs express numerous G protein-coupled receptors (GPCRs), which are the largest group of membrane receptor proteins and the most common targets of drug therapy...
September 2016: Journal of Allergy and Clinical Immunology
Yuanzhen Chen, Shuyan Jiang, Yuying Liu, Jialing Xiong, Jiexian Liang, Wenjin Ji
It has previously been demonstrated that bradykinin receptor B1 (B1R) agonists evoke an itch-related scratching response in inflamed skin via the B1 receptor; however, the mechanisms responsible for this abnormal itch sensation remain unclear. Therefore, the present study utilized a complete Freund's adjuvant (CFA)-induced mouse model of inflammation to elucidate the mechanisms responsible. Over a period of 30 min, scratching behavior was quantified by the number of hind limb scratches of the area surrounding the drug injection site on the neck...
August 2016: Experimental and Therapeutic Medicine
Vinod S Hegade, Stuart F W Kendrick, Robert L Dobbins, Sam R Miller, Duncan Richards, James Storey, George Dukes, Kim Gilchrist, Susan Vallow, Graeme J Alexander, Margaret Corrigan, Gideon M Hirschfield, David E J Jones
BACKGROUND: Pruritus (itch) is a symptom commonly experienced by patients with cholestatic liver diseases such as primary biliary cholangitis (PBC, previously referred to as primary biliary cirrhosis). Bile acids (BAs) have been proposed as potential pruritogens in PBC. The ileal bile acid transporter (IBAT) protein expressed in the distal ileum plays a key role in the enterohepatic circulation of BAs. Pharmacological inhibition of IBAT with GSK2330672 may reduce BA levels in the systemic circulation and improve pruritus...
2016: BMC Gastroenterology
Magdalena Trzeciak, Monika Sakowicz-Burkiewicz, Martyna Wesserling, Daria Dobaczewska, Jolanta Gleń, Roman Nowicki, Tadeusz Pawelczyk
Changes in the expression of cornified envelope (CE) proteins are thought to affect the development and course of atopic dermatitis (AD). The aim of this study was to examine the expression level of CE proteins in order to identify new molecular markers of the AD phenotype. Expression levels of CE proteins were evaluated in the skin of patients with AD (38 biopsies) and healthy subjects (26 biopsies). Levels of FLG, FLG2 and SPRR3 mRNAs and proteins were reduced in AD skin. Levels of LELP-1 and SPRR1A transcripts and proteins were significantly increased in AD skin...
June 15, 2016: Acta Dermato-venereologica
Aleksander Obtułowicz, Magdalena Pirowska, Anna Wojas-Pelc
INTRODUCTION: Protein contact dermatitis (PCD) is an IgE-dependent allergic reaction which, despite enormous progress in knowledge, remains a 'non-diagnosed' nosologic unit in contemporary medicine. Skin lesion, with a chronic and recurring course, are analogous with the clinical picture in allergic contact dermatitis (ACD) and irritant contact dermatitis (ICD); skin patch tests, however, are usually negative. This makes the diagnostics difficult, prevents a correct diagnosis and treatment based on the avoidance of allergen...
June 2, 2016: Annals of Agricultural and Environmental Medicine: AAEM
Dafni-Eleftheria Pefani, Daniela Pankova, Aswin G Abraham, Anna M Grawenda, Nikola Vlahov, Simon Scrace, Eric O' Neill
Epigenetic inactivation of the Hippo pathway scaffold RASSF1A is associated with poor prognosis in a wide range of sporadic human cancers. Loss of expression reduces tumor suppressor activity and promotes genomic instability, but how this pleiotropic biomarker is regulated at the protein level is unknown. Here we show that TGF-β is the physiological signal that stimulates RASSF1A degradation by the ubiquitin-proteasome pathway. In response to TGF-β, RASSF1A is recruited to TGF-β receptor I and targeted for degradation by the co-recruited E3 ubiquitin ligase ITCH...
July 7, 2016: Molecular Cell
Andrew M Bell, Maria Gutierrez-Mecinas, Erika Polgár, Andrew J Todd
BACKGROUND: Gastrin-releasing peptide (GRP) is thought to play a role in the itch evoked by intradermal injection of chloroquine. Although some early studies suggested that GRP was expressed in pruriceptive primary afferents, it is now thought that GRP in the spinal cord is derived mainly from a population of excitatory interneurons in lamina II, and it has been suggested that these are involved in the itch pathway. To test this hypothesis, we used the transcription factor Fos and phosphorylation of extracellular signal-regulated kinases (ERK) to look for evidence that interneurons expressing GRP were activated following intradermal injection of chloroquine into the calf, in mice that express enhanced green fluorescent protein (EGFP) in these cells...
2016: Molecular Pain
Rong Wang, Aizhang Xu, Xueying Zhang, Jie Wu, Andrew Freywald, Jianqing Xu, Jim Xiang
CD8(+) cytotoxic T lymphocyte (CTL) exhaustion is a chief issue for ineffective virus elimination in chronic infectious diseases. We generated novel ovalbumin (OVA)-specific OVA-Texo and HIV-specific Gag-Texo vaccines inducing therapeutic immunity. To assess their therapeutic effect in chronic infection, we developed a new chronic infection model by i.v. infecting C57BL/6 mice with the OVA-expressing adenovirus AdVova. During chronic AdVova infection, mouse CTLs were found to express the inhibitory molecules programmed cell-death protein-1 (PD-1) and lymphocyte-activation gene-3 (LAG-3) and to be functionally exhausted, showing a significant deficiency in T-cell proliferation, IFN-γ production and cytolytic effects...
June 6, 2016: Cellular & Molecular Immunology
Haiwen Li, Heng Zhu, Yang Liu, Fuchu He, Ping Xie, Lingqiang Zhang
Protein neddylation is essential for the viability of most organisms and is widely involved in the regulation of immunity, DNA damage and repair, cell signaling and cell cycle. Unlike RING-type neddylation ligases, HECT-type neddylation ligase remains less defined. Here, we show that Itch is a novel HECT-type neddylation E3 ligase and we identify JunB as a substrate of Nedd8 modification by Itch. JunB neddylation attenuates its transcriptional activity. In addition, JunB neddylation mediated by Itch promotes its ubiquitination-dependent degradation...
September 2016: Cellular Signalling
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