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https://www.readbyqxmd.com/read/29449647/the-pro-inflammatory-phenotype-of-the-human-non-classical-monocyte-subset-is-attributed-to-senescence
#1
Siew-Min Ong, Eva Hadadi, Truong-Minh Dang, Wei-Hseun Yeap, Crystal Tze-Ying Tan, Tze-Pin Ng, Anis Larbi, Siew-Cheng Wong
Human primary monocytes comprise a heterogeneous population that can be classified into three subsets based on CD14 and CD16 expression: classical (CD14 high /CD16 - ), intermediate (CD14 high /CD16 + ), and non-classical (CD14 low /CD16 + ). The non-classical monocytes are the most pro-inflammatory in response to TLR stimulation in vitro, yet they express a remarkably high basal level of miR-146a, a microRNA known to negatively regulate the TLR pathway. This concurrence of a pro-inflammatory status and a high miR-146a level has been associated with cellular senescence in other cell types...
February 15, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29449276/immune-evasion-via-pd-1-pd-l1-on-nk-cells-and-monocyte-macrophages-is-more-prominent-in-hodgkin-lymphoma-than-dlbcl
#2
Frank Vari, David Arpon, Colm Keane, Mark S Hertzberg, Dipti Talaulikar, Sanjiv Jain, Qingyan Cui, Erica Han, Josh Tobin, Robert Bird, Donna Cross, Annette Hernandez, Clare Gould, Simone Birch, Maher K Gandhi
Much focus has been on the interaction of PD-L1 on malignant B-cells with PD-1 on effector T-cells in inhibiting anti-lymphoma immunity. We sought to establish the contribution of NK-cells and inhibitory CD163 + monocytes/macrophages in Hodgkin Lymphoma (cHL) and Diffuse Large B-cell Lymphoma (DLBCL). Levels of PD-1 on NK-cells were elevated in cHL relative to DLBCL. Notably, CD3 - CD56 hi CD16 -ve NK-cells had substantially higher PD-1 expression relative to CD3 - CD56 dim CD16 + cells, and were expanded in blood and tissue, more marked in cHL than DLBCL patients...
February 15, 2018: Blood
https://www.readbyqxmd.com/read/29448007/dysfunction-of-cd3-cd16-cd56-dim-and-cd3-cd16-cd56-bright-nk-cell-subsets-in-rr-ms-patients
#3
Ilhan Tahrali, Umut Can Kucuksezer, Ayse Altintas, Ugur Uygunoglu, Nilgun Akdeniz, Esin Aktas Cetin, Gunnur Deniz
Multiple sclerosis (MS), is a chronic inflammatory disease of central nervous system with unclear etiology. Relapsing-remitting (RR)-MS is the most frequent subtype of disease. Natural Killer (NK) cells have roles in cytotoxicity and immune regulation by cytokine secretions, with uncertain contribution to MS pathogenesis. This study aimed to explore contribution of NK cells to MS pathogenesis. Percentages of CD3 - CD16 + CD56 + total NK cells, CD3 - CD16 + CD56 dim and CD3 - CD16 - CD56 bright NK cell subsets, NK cytotoxicity and intracellular IFN-γ, IL-10 and IL-22 levels were investigated in patients with RR-MS and clinically isolated syndrome (CIS) as well as healthy subjects...
February 12, 2018: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29435587/a-disease-associated-microrna-cluster-links-inflammatory-pathways-and-an-altered-composition-of-leukocyte-subsets-to-noninfectious-uveitis
#4
Fleurieke H Verhagen, Cornelis P J Bekker, Marzia Rossato, Sanne Hiddingh, Lieuwe de Vries, Abhinandan Devaprasad, Aridaman Pandit, Jeannette Ossewaarde-van Norel, Ninette Ten Dam, Maartje C A Moret-Pot, Saskia M Imhof, Joke H de Boer, Timothy R D J Radstake, Jonas J W Kuiper
Purpose: The cause of noninfectious uveitis (NIU) is poorly understood but is considered to be mediated by a complex interplay between genetic, environmental, and-relatively unexplored-epigenetic factors. MicroRNAs (miRNAs) are noncoding small RNAs that are important epigenetic regulators implicated in pathologic signaling. Therefore, we mapped the circulating miRNA-ome of NIU patients and studied miRNA perturbations within the broader context of the immune system. Methods: We designed a strategy to robustly identify changes in the miRNA profiles of two independent cohorts totaling 54 untreated patients with active and eye-restricted disease and 26 age-matched controls...
February 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29434775/il-25-promotes-th2-bias-by-upregulating-il-4-and-il-10-expression-of-decidual-%C3%AE-%C3%AE-t-cells-in-early-pregnancy
#5
Yuan Zhang, Ying Wang, Ming-Qing Li, Jie Duan, Deng-Xuan Fan, Li-Ping Jin
Decidual immune cells (DICs), consisting of both innate and adaptive immune cells, have a pivotal role in maintaining immune tolerance for normal pregnancy. Our previous study demonstrated that interleukin (IL)-25 stimulates the proliferation of decidual stromal cells (DSCs) in an autocrine manner. However, the role of IL-25 in functional regulation of DICs is largely unknown. Flow cytometry was used to analyze the expression of IL-25 and its receptor (IL-17RB) in DICs, and the effect of IL-25 on the expression of Ki-67, IL-4, IL-10, interferon (IFN)-γ and transforming growth factor (TGF)-β in decidual γδT cells...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29423944/impact-of-mon2-monocyte-platelet-aggregates-in-humancoronary-artery-disease
#6
Richard A Brown, Gregory Y H Lip, Chetan Varma, Eduard Shantsila
INTRODUCTION: Monocyte-platelet aggregates (MPAs) form when Mon1, Mon2 or Mon3 monocyte subsets adhere to platelets. They are pathophysiologically linked to coronary artery disease (CAD). However, their individual roles in the occurrence of diffuse CAD remain unknown. METHODS: Peripheral blood from 50 patients with diffuse CAD, 40 patients with focal CAD and 50 age-matched patients with normal coronary arteries was analysed by flow cytometry to quantify MPAs associated with individual monocyte subsets...
February 9, 2018: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29416010/role-of-c-abl-and-nephrin-in-podocyte-cytoskeletal-remodeling-induced-by-angiotensin-ii
#7
Yiqiong Ma, Qian Yang, Zhentong Zhong, Wei Liang, Lu Zhang, Yingjie Yang, Guohua Ding
Our previous study showed that angiotensin II (Ang II) exposure diminished the interaction between nephrin and c-Abl, then c-Abl mediated SHIP2-Akt pathway in the process of podocyte injury in vivo and vitro. However, the relationship between nephrin and c-Abl was unknown. Recently, various studies showed that nephrin was required for cytoskeletal remodeling in glomerular podocytes. But its specific mechanisms remain incompletely understood. As a nonreceptor tyrosine kinase involved in cytoskeletal regulation, c-Abl may be a candidate of signaling proteins interacting with Src homology 2/3 (SH2/SH3) domains of nephrin...
February 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29414654/interleukin-8-favors-pro-inflammatory-activity-of-human-monocytes-macrophages
#8
Maksim Evgenievich Meniailo, Vladimir Vladimirovich Malashchenko, Vyacheslav Anatolievich Shmarov, Natalia Dinislamovna Gazatova, Olga Borisovna Melashchenko, Andrei Gennadievich Goncharov, Galina Viktorovna Seledtsova, Viktor Ivanovich Seledtsov
Interleukin-8 (IL-8, CXCL8) belongs to major chemokines to stimulate migration of neutrophils and monocytes/macrophages (Mc/Mphs) into the inflammation sites. We studied the direct effects of IL-8 on the functionality of human Mc/Mphs in vitro. CD14-positive cells were isolated from human peripheral blood mononuclear cells (PBMCs) by positive magnetic separation and were further cultured with or without lipopolysaccharide (LPS, 1.0 μg/ml) for 24 h. We showed that upon LPS activation of Mc/Mphs, IL-8 reduced markedly both the percentages and median fluorescence intensity (MFI) of CD16 (FcγRIII)-positive cells among CD14high cells, as well as in cells that reduced the expression of СD14 during their culturing...
January 27, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29407600/impact-of-cd14-cd16-monocytes-on-coronary-plaque-vulnerability-assessed-by-optical-coherence-tomography-in-coronary-artery-disease-patients
#9
Hiroyuki Yamamoto, Naofumi Yoshida, Toshiro Shinke, Hiromasa Otake, Masaru Kuroda, Kazuhiko Sakaguchi, Yushi Hirota, Takayoshi Toba, Hachidai Takahashi, Daisuke Terashita, Kenzo Uzu, Natsuko Tahara, Yuto Shinkura, Kouji Kuroda, Yoshinori Nagasawa, Yuichiro Nagano, Yoshiro Tsukiyama, Ken-Ichi Yanaka, Takuo Emoto, Naoto Sasaki, Tomoya Yamashita, Wataru Ogawa, Ken-Ichi Hirata
BACKGROUND AND AIMS: This study examined the impact of CD14++CD16+ monocytes on coronary plaque vulnerability, as assessed by optical coherence tomography (OCT), and investigated their association with daily glucose fluctuation. Although increased CD14++CD16+ monocyte levels have been reported to increase cardiovascular events, their impact on coronary plaque vulnerability in coronary artery disease (CAD) patients with or without diabetes mellitus (DM) remains unclear. METHODS: This prospective observational study included 50 consecutive patients with CAD, receiving lipid-lowering therapy and undergoing coronary angiography and OCT...
January 17, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29407575/anti-inflammatory-effects-of-anisalcohol-on-lipopolysaccharide-stimulated-bv2-microglia-via-selective-modulation-of-microglia-polarization-and-down-regulation-of-nf-%C3%AE%C2%BAb-p65-and-jnk-activation
#10
Bin Xiang, Chun Xiao, Ting Shen, Xiufang Li
Inflammation plays a pivotal role in the pathogenesis of ischemic stroke. The inhibition of inflammation appears to be a potential therapeutic strategy for neuro-inflammatory injury after ischemic stroke. In response to cerebral ischemia, resident microglia and infiltrated macrophages from the damaged blood-brain barrier are activated. Microglia activation appears to be a double-edged sword. Activated microglia migrate to the damaged neuron, change their phenotype to M1 or M2, and become involved in nerve damage and repair...
January 29, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29401756/in-depth-proteomic-characterization-of-classical-and-non-classical-monocyte-subsets
#11
Víctor Segura, M Luz Valero, Laura Cantero, Javier Muñoz, Eduardo Zarzuela, Fernando García, Kerman Aloria, Javier Beaskoetxea, Jesús M Arizmendi, Rosana Navajas, Alberto Paradela, Paula Díez, Rosa Mª Dégano, Manuel Fuentes, Alberto Orfao, Andrés García Montero, Alba Garin-Muga, Fernando J Corrales, Manuel M Sánchez Del Pino
Monocytes are bone marrow-derived leukocytes that are part of the innate immune system. Monocytes are divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16. These cells are key players in the inflammation process underlying the mechanism of many diseases. Thus, the molecular characterization of these cells may provide very useful information for understanding their biology in health and disease. We performed a multicentric proteomic study with pure classical and non-classical populations derived from 12 healthy donors...
February 5, 2018: Proteomes
https://www.readbyqxmd.com/read/29400603/a-novel-bicistronic-gene-design-couples-stable-cell-line-selection-with-a-fucose-switch-in-a-designer-cho-host-to-produce-native-and-afucosylated-glycoform-antibodies
#12
Gargi Roy, Tom Martin, Arnita Barnes, Jihong Wang, Rod Brian Jimenez, Megan Rice, Lina Li, Hui Feng, Shu Zhang, Raghothama Chaerkady, Herren Wu, Marcello Marelli, Diane Hatton, Jie Zhu, Michael A Bowen
The conserved glycosylation site Asn297 of a monoclonal antibody (mAb) can be decorated with a variety of sugars that can alter mAb pharmacokinetics and recruitment of effector proteins. Antibodies lacking the core fucose at Asn297 (afucosylated mAbs) show enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) and increased efficacy. Here, we describe the development of a robust platform for the manufacture of afucosylated therapeutic mAbs by engineering a Chinese hamster ovary (CHO) host cell line to co-express a mAb with GDP-6-deoxy-D-lyxo-4-hexulose reductase (RMD), a prokaryotic enzyme that deflects an intermediate in the de novo synthesis of fucose to a dead-end product, resulting in the production of afucosylated mAb (GlymaxX™ Technology, ProBioGen)...
February 5, 2018: MAbs
https://www.readbyqxmd.com/read/29399160/microglia-immunophenotyping-in-gliomas
#13
Laura Annovazzi, Marta Mellai, Enrica Bovio, Samanta Mazzetti, Bianca Pollo, Davide Schiffer
Microglia, once assimilated to peripheral macrophages, in gliomas has long been discussed and currently it is hypothesized to play a pro-tumor role in tumor progression. Uncertain between M1 and M2 polarization, it exchanges signals with glioma cells to create an immunosuppressive microenvironment and stimulates cell proliferation and migration. Four antibodies are currently used for microglia/macrophage identification in tissues that exhibit different cell forms and cell localization. The aim of the present work was to describe the distribution of the different cell forms and to deduce their significance on the basis of what is known on their function from the literature...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29396991/alterations-in-natural-killer-and-dendritic-cell-subsets-in-individuals-with-hiv-associated-neurotuberculosis
#14
Deepashri Rao, Manjunatha M Venkataswamy, Ravi Vasanthapuram, P Satishchandra, Anita Desai
One of the commonest HIV-associated opportunistic infections of the central nervous system is neurotuberculosis. Interaction between HIV, Mycobacterium tuberculosis and host immune system in co-infected individuals may result in altered frequencies of immune cells, thereby modulating dissemination and disease progression. We examined the frequencies of natural killer (NK) cell and dendritic cell (DC) subsets in HIV infected individuals with neurotuberculosis (HIVNTB) as compared to individuals with HIV associated systemic TB (HIVSTB), asymptomatic HIV, non-HIV NTB, non-HIV STB and healthy controls...
February 3, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29395574/up-regulated-expression-of-cd86-on-circulating-intermediate-monocytes-correlated-with-disease-severity-in-psoriasis
#15
Chuyen Thi Hong Nguyen, Naotomo Kambe, Fumikazu Yamazaki, Ikuko Ueda-Hayakawa, Izumi Kishimoto, Hiroyuki Okamoto
BACKGROUND: The number of intermediate monocytes (CD14++CD16+) increases in many inflammatory conditions. However, it is not yet known which functional markers expressed by these populations are linked to the pathogenesis of psoriasis. OBJECTIVES: We evaluated the expression of functional markers on circulating intermediate monocytes. Our goal was to correlate specific populations and their markers with the clinical severity of psoriasis. METHODS: A cohort of 43 psoriatic patients was subjected to analysis...
January 27, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29388200/phase-i-clinical-trial-of-adoptive-transfer-of-expanded-natural-killer-cells-in-combination-with-igg1-antibody-in-patients-with-gastric-or-colorectal-cancer
#16
Takeshi Ishikawa, Tetsuya Okayama, Naoyuki Sakamoto, Mitsuko Ideno, Kaname Oka, Tatsuji Enoki, Junichi Mineno, Naohisa Yoshida, Kazuhiro Katada, Kazuhiro Kamada, Kazuhiko Uchiyama, Osamu Handa, Tomohisa Takagi, Hideyuki Konishi, Satoshi Kokura, Kazuko Uno, Yuji Naito, Yoshito Itoh
Natural killer (NK) cells exhibit strong cytotoxic activity against tumor cells without prior sensitization, and have the potential to exert antibody-dependent cellular cytotoxicity (ADCC). In this clinical trial, we examined the safety and efficacy of the use of NK cells, generated using a novel expansion system, in combination with IgG1 antibodies for the treatment of advanced gastric or colorectal cancers. Treatment consisted of trastuzumab- or cetuximab-based chemotherapy, plus adoptive NK cell therapy...
January 31, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29388006/glycan-recognition-by-human-blood-mononuclear-cells-with-an-emphasis-on-dendritic-cells
#17
Eugenia M Rapoport, Sergey V Khaidukov, Andrey M Gaponov, Galina V Pazynina, Svetlana V Tsygankova, Ivan M Ryzhov, Ivan M Belyanchikov, Panagiota Milona, Nicolai V Bovin, Kenneth C McCullough
Dendritic cells (DCs) play crucial roles in innate and adaptive immune response, for which reason targeting antigen to these cells is an important strategy for improvement of vaccine development. To this end, we explored recognition of DCs lectins by glycans. For selection of the glycan "vector", a library of 229 fluorescent glycoprobes was employed to assess interaction with the CD14low/-CD16+CD83+ blood mononuclear cell population containing the DCs known for their importance in antigen presentation to T-lymphocytes...
January 31, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29383775/diagnostic-validity-of-flow-cytometry-vs-manual-counting-of-polymorphonuclear-leukocytes-in-spontaneous-bacterial-peritonitis
#18
Heba Ahmed Osman, Sanaa Shaker Aly, Eman M Salah-Eldin, Muhammad Abbas El-Masry, Mohammed H Hassan
BACKGROUND: Spontaneous bacterial peritonitis (SBP) is frequently occurring infection among patients with liver cirrhosis, defined by polymorphonuclear (PMN) leukocytic count ≥250 cell/mm3 with or without a positive ascitic fluid (AF) bacterial culture. So, this study aimed to investigate the diagnostic value of flow cytometry versus manual counting of ascitic fluid PMNL in cirrhotic patients, with clinical suspicion of SBP. METHODS: A hospital-based cross-sectional study was carried out on 320 cirrhotic patients with clinical suspicion of SBP...
January 31, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29381229/time-to-event-modelling-of-effect-of-codrituzumab-on-overall-survival-in-patients-with-hepatocellular-carcinoma
#19
Mikiko Nakamura, Chao Xu, Cheikh Diack, Norihisa Ohishi, Ruey-Min Lee, Satofumi Lida, Takehiko Kawanishi, Toshihiko Ohtomo, Ghassan K Abou-Alfa, Ya-Chi Chen
AIMS: Codrituzumab (GC33) is a recombinant, humanized mAb that binds to glypican-3 (GPC3), an oncofoetal protein highly expressed in hepatocellular carcinoma (HCC). This investigation aimed to identify clinically relevant factors that may affect the overall survival (OS) in HCC patients treated with codrituzumab and to quantitatively annotate their effects. METHODS: Codrituzumab exposure was estimated by a population pharmacokinetics model with a non-linear elimination pathway...
January 30, 2018: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29374020/blood-monocyte-frequency-may-be-a-biomarker-for-response-to-anti-pd-1
#20
(no author information available yet)
CD14+CD16-HLA-DRhi monocyte frequency was linked to response to anti-PD-1 in patients with melanoma.
January 26, 2018: Cancer Discovery
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