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https://www.readbyqxmd.com/read/28110421/dendritic-cell-immune-responses-in-hiv-1-controllers
#1
REVIEW
Enrique Martin-Gayo, Xu G Yu
PURPOSE OF REVIEW: Robust HIV-1-specific CD8 T cell responses are currently regarded as the main correlate of immune defense in rare individuals who achieve natural, drug-free control of HIV-1; however, the mechanisms that support evolution of such powerful immune responses are not well understood. Dendritic cells (DCs) are specialized innate immune cells critical for immune recognition, immune regulation, and immune induction, but their possible contribution to HIV-1 immune defense in controllers remains ill-defined...
January 21, 2017: Current HIV/AIDS Reports
https://www.readbyqxmd.com/read/28110402/muscular-atrophy-in-severe-cases-of-cubital-tunnel-syndrome-prognostic-factors-and-outcome-after-surgical-treatment
#2
Markus Bruder, Stephan Dützmann, Nourdin Rekkab, Johanna Quick, Volker Seifert, Gerhard Marquardt
BACKGROUND: Cubital tunnel syndrome (CuTS) is a frequent neuropathy, leading to sensor-motoric dysfunction. Many patients even present with muscular atrophy as a sign for severe and long-lasting nerve impairment, usually suggesting unfavourable outcome. We analysed if those patients benefit from surgical treatment on a long-term basis. METHODS: Between January 2010 and March 2015, 42 consecutive cases of CuTS with atrophy of the intrinsic hand muscles were surgically treated in our department...
January 21, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28110263/multi-mhz-micro-electro-mechanical-sensors-for-atomic-force-microscopy
#3
Bernard Legrand, Jean-Paul Salvetat, Benjamin Walter, Marc Faucher, Didier Théron, Jean-Pierre Aimé
Silicon ring-shaped micro-electro-mechanical resonators have been fabricated and used as probes for dynamic atomic force microscopy (AFM) experiments. They offer resotnance frequency above 10MHz, which is notably greater than that of usual cantilevers and quartz-based AFM probes. On-chip electrical actuation and readout of the tip oscillation are obtained by means of built-in capacitive transducers. Displacement and force resolutions have been determined from noise analysis at 1.5fm/√Hz and 0.4 pN/√Hz, respectively...
January 12, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28110244/quantification-of-postural-control-deficits-in-patients-with-recent-concussion-an-inertial-sensor-based-approach
#4
Cailbhe Doherty, Liang Zhao, John Ryan, Yusuke Komaba, Akihiro Inomata, Brian Caulfield
BACKGROUND: The aim of this study was to quantify postural control ability in a group with concussion compared with a healthy control group. METHOD: Fifteen concussion patients (4 females, 11 males) and a group of fifteen age- and sex-matched controls were recruited. Participants were tested during the performance of the three stance variants (bilateral, tandem and unilateral) of the balance error scoring system standing on a force place, while wearing an inertial measurement unit placed at the posterior aspect of the sacrum...
January 16, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28109965/a-novel-acidic-ph-fluorescent-probe-based-on-a-benzothiazole-derivative
#5
Qiujuan Ma, Xian Li, Suxiang Feng, Beibei Liang, Tiqiang Zhou, Min Xu, Zhuoyi Ma
A novel acidic pH fluorescent probe 1 based on a benzothiazole derivative has been designed, synthesized and developed. The linear response range covers the acidic pH range from 3.44 to 6.46, which is valuable for pH researches in acidic environment. The evaluated pKa value of the probe 1 is 4.23. The fluorescence enhancement of the studied probe 1 with an increase in hydrogen ions concentration is based on the hindering of enhanced photo-induced electron transfer (PET) process. Moreover, the pH sensor possesses a highly selective response to H(+) in the presence of metal ions, anions and other bioactive small molecules which would be interfere with its fluorescent pH response...
January 12, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28109758/the-use-of-electronic-sensor-device-to-augment-ligament-balancing-leads-to-a-lower-rate-of-arthrofibrosis-after-total-knee-arthroplasty
#6
Jeffrey A Geller, Akshay Lakra, Taylor Murtaugh
BACKGROUND: Total knee arthroplasty (TKA) is a highly successful surgery shown to improve quality of life. One of the more common known complications of TKA is early arthrofibrosis requiring manipulation under anesthesia (MUA). This investigation evaluates the incidence of arthrofibrosis before and after the implementation of an electronic sensor device used to assist with ligament balancing. METHODS: Six hundred ninety TKAs performed without sensor use were compared to a cohort of 252 TKAs performed with sensor usage...
December 24, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28109721/inflammasome-priming-in-sterile-inflammatory-disease
#7
REVIEW
Meghana N Patel, Richard G Carroll, Silvia Galván-Peña, Evanna L Mills, Robin Olden, Martha Triantafilou, Amaya I Wolf, Clare E Bryant, Kathy Triantafilou, Seth L Masters
The inflammasome is a cytoplasmic protein complex that processes interleukins (IL)-1β and IL-18, and drives a form of cell death known as pyroptosis. Oligomerization of this complex is actually the second step of activation, and a priming step must occur first. This involves transcriptional upregulation of pro-IL-1β, inflammasome sensor NLRP3, or the non-canonical inflammasome sensor caspase-11. An additional aspect of priming is the post-translational modification of particular inflammasome constituents...
January 18, 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/28108943/is-the-assessment-of-5-meters-of-gait-with-a-single-body-fixed-sensor-enough-to-recognize-idiopathic-parkinson-s-disease-associated-gait
#8
M E Micó-Amigo, I Kingma, G S Faber, A Kunikoshi, J M T van Uem, R C van Lummel, W Maetzler, J H van Dieën
Quantitative assessment of gait in patients with Parkinson's disease (PD) is an important step in addressing motor symptoms and improving clinical management. Based on the assessment of only 5 meters of gait with a single body-fixed-sensor placed on the lower back, this study presents a method for the identification of step-by-step kinematic parameters in 14 healthy controls and in 28 patients at early-to-moderate stages of idiopathic PD. Differences between groups in step-by-step kinematic parameters were evaluated to understand gait impairments in the PD group...
January 20, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28108754/using-fluorescence-immunochromatographic-test-strips-based-on-quantum-dots-for-the-rapid-and-sensitive-determination-of-microcystin-lr
#9
Jiadi Sun, Ying Li, Fuwei Pi, Jian Ji, Yinzhi Zhang, Xiulan Sun
A novel immunosensor for the detection of microcystin-LR (MC-LR) was constructed with use of immunochromatographic test strips (ICTS). Quantum dots were chosen to be the fluorescent labels for the immune sensor in ICTS because of their excellent optical and electronic properties with a relatively narrow emission spectrum. The detection sensitivity of the ICTS was related to the concentration of the fluorescent probe and the amount of the MC-LR standards. Under optimal conditions, with MC-LR as the target, the ICTS sensor had a linear range from 0...
January 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28108644/bicarbonate-sensing-soluble-adenylyl-cyclase-is-present-in-the-cell-cytoplasm-and-nucleus-of-multiple-shark-tissues
#10
Jinae N Roa, Martin Tresguerres
The enzyme soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate (HCO3(-)) to produce the signaling molecule cyclic adenosine monophosphate (cAMP). Because sAC and sAC-related enzymes are found throughout phyla from cyanobacteria to mammals and they regulate cell physiology in response to internal and external changes in pH, CO2, and HCO3(-), sAC is deemed an evolutionarily conserved acid-base sensor. Previously, sAC has been reported in dogfish shark and round ray gill cells, where they sense and counteract blood alkalosis by regulating the activity of V-type H(+)- ATPase...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28108102/effects-of-soybean-lipid-infusion-on-unbound-free-fatty-acids-and-unbound-bilirubin-in-preterm-infants
#11
Thomas Hegyi, Alan Kleinfeld, Andrew Huber, Barry Weinberger, Naureen Memon, Weichung Joe Shih, Mary Carayannopoulos, William Oh
OBJECTIVE: To assess the effects of a soybean lipid emulsion infusions on levels of unbound (free) bilirubin (Bf) and unbound free fatty acids (FFAu) as well as changes in Bf and total serum bilirubin (TSB) during phototherapy in infants born preterm. STUDY DESIGN: Ninety-seven infants born preterm (birth weight: 500-2000 g; gestational age: 23-34 weeks) were enrolled to investigate the effect of 0, 1, 2, and 3 g/kg/d of intralipid infusion on Bf and FFAu. Pre- and postphototherapy TSB, FFAu, and Bf also were analyzed in 91 infants to assess the effects of phototherapy...
January 12, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28107987/generation-of-biostable-l-aptamers-against-achiral-targets-by-chiral-inversion-of-existing-d-aptamers
#12
Huapei Chen, Sitao Xie, Hao Liang, Cuichen Wu, Liang Cui, Shuang-Yan Huan, Xiaobing Zhang
In this paper, based on reciprocal chiral substrate specificity, taking achiral molecules, ethanolamine (EA) and malachite green (MG) as two model targets, biostable L- DNA aptamers and L-RNA aptamers were generated respectively by chiral inversion of existing D-aptamers. In the detection of EA with L-DNA aptamer-based sensors, the feasibility of our strategy was confirmed, while in the detection of MG with L-RNA aptamers, linear calibration curves were obtained in the range from 0.1 to 5µm with the detection limit of 0...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107983/characterization-of-inclusion-complexes-of-organic-ions-with-hydrophilic-hosts-by-ion-transfer-voltammetry-with-solvent-polymeric-membranes
#13
José Manuel Olmos, Eduardo Laborda, Joaquín Ángel Ortuño, Ángela Molina
The quantitative characterization of inclusion complexes formed in aqueous phase between organic ions and hydrophilic hosts by ion-transfer voltammetry with solvent polymeric membrane ion sensors is studied, both in a theoretical and experimental way. Simple analytical solutions are presented for the determination of the binding constant of the complex from the variation with the host concentration of the electrochemical signal. These solutions are valid for any voltammetric technique and for solvent polymeric membrane ion sensors comprising one polarisable interface (1PI) and also, for the first time, two polarisable interfaces (2PIs)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107979/electrochemical-sensing-platform-based-on-molecularly-imprinted-polymer-decorated-n-s-co-doped-activated-graphene-for-ultrasensitive-and-selective-determination-of-cyclophosphamide
#14
Bintong Huang, Lili Xiao, Haifeng Dong, Xueji Zhang, Wei Gan, Shahid Mahboob, Khalid Abdullah Al-Ghanim, Qunhui Yuan, Yingchun Li
A novel electrochemical sensor has been facilely fabricated by applying composite elements consisting of nitrogen and sulfur co-doped activated graphene (N,S-AGR) and molecularly imprinted polymer (MIP). N,S-AGR was synthesized by one-pot pyrogenation of a mixture of thiourea, KOH and graphene oxide, which was introduced to improve electron transfer capability and surface area of the electrode, while the electro-polymerized MIP layer afforded simultaneous recognition and quantification of cyclophosphamide (CPA) by utilizing Fe(CN)6(3-/4-) as probe to indicate electrical signals...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107972/electrochemical-sensor-for-sensitive-detection-of-triclosan-based-on-graphene-palladium-nanoparticles-hybrids
#15
Tingxuan Wu, Tingting Li, Zhiguang Liu, Yujing Guo, Chuan Dong
In this paper, poly (diallyldimethylammonium chloride) functionalized graphene/palladium nanoparticles (PDDA-Gr/PdNPs) have been successfully synthesized through a simple one-pot method with the use of PDDA as stabilizing agents. The electrochemical response of triclosan on the PDDA-Gr/PdNPs modified glassy carbon electrode (GCE) is greatly enhanced comparing to the PDDA-Gr/GCE or the bare GCE. It is suggested that the nanocomposite exhibited good electron transfer ability and catalytic activity. Under the optimal conditions, the oxidation current increased linearly with the concentration of triclosan in the range of 9...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107967/effect-of-ordered-mesoporous-carbon-contact-layer-on-the-sensing-performance-of-sputtered-ruo2-thin-film-ph-sensor
#16
W Lonsdale, D K Maurya, M Wajrak, K Alameh
The effect of contact layer on the pH sensing performance of a sputtered RuO2 thin film pH sensor is investigated. The response of pH sensors employing RuO2 thin film electrodes on screen-printed Pt, carbon and ordered mesoporous carbon (OMC) contact layers are measured over a pH range from 4 to 10. Working electrodes with OMC contact layer are found to have Nernstian pH sensitivity (-58.4mV/pH), low short-term drift rate (5.0mV/h), low hysteresis values (1.13mV) and fast reaction times (30s), after only 1h of conditioning...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107966/novel-potentiometric-sensors-for-the-determination-of-the-dinotefuran-insecticide-residue-levels-in-cucumber-and-soil-samples
#17
Maha F Abdel-Ghany, Lobna A Hussein, Noha F El Azab
Five new potentiometric membrane sensors for the determination of the dinotefuran levels in cucumber and soil samples have been developed. Four of these sensors were based on a newly designed molecularly imprinted polymer (MIP) material consisting of acrylamide or methacrylic acid as a functional monomer in a plasticized PVC (polyvinyl chloride) membrane before and after elution of the template. A fifth sensor, a carboxylated PVC-based sensor plasticized with dioctyl phthalate, was also prepared and tested...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107958/one-step-synthesis-of-graphitic-carbon-nitride-nanosheets-with-the-help-of-melamine-and-its-application-for-fluorescence-detection-of-mercuric-ions
#18
Qianfen Zhuang, Liming Sun, Yongnian Ni
A facile, simple, and relatively environment-friendly hydrothermal approach was developed for one-step synthesis of graphitic carbon nitride nanosheets (GCNNs) using melamine and sodium citrate as the precursors. The prepared GCNNs emit strong fluorescence with a high quantum yield of 48.3%. The GCNNs were then characterized by various techniques including transmission electron microscopy, atomic force microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and UV-Vis absorption spectroscopy...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107936/a-simple-and-sensitive-method-for-determination-of-taxifolin-on-palladium-nanoparticles-supported-poly-diallyldimethylammonium-chloride-functionalized-graphene-modified-electrode
#19
Qinqin Wang, Lu Wang, Gaiping Li, Baoxian Ye
Palladium nanoparticles with diameter of around 5-10nm supported on poly (diallyldimethylammonium chloride) (PDDA)-functionalized graphene composites were prepared by a simple chemical method. As-prepared nanocomposites were characterized by transmission electron microscopy (TEM), x-ray diffraction (XRD), ultraviolet-visible spectrophotometry (UV-vis) and electrochemical techniques, which were clearly demonstrated that palladium nanoparticles were uniformly dispersed on the graphene sheets. Based on the PDDA-Gr-Pd nanocomposites, a new voltammetric sensor was established and exhibited excellent electrocatalytic activity and fast electron transfer rate toward taxifolin...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107932/mesoporous-nico2o4-decorated-reduced-graphene-oxide-as-a-novel-platform-for-electrochemical-determination-of-rutin
#20
Shiqiang Cui, Li Li, Yaping Ding, Jiangjiang Zhang, Hua Yang, Yingzi Wang
The glassy carbon electrode (GCE) modified with mesoporous NiCo2O4-decorated reduced graphene oxide (NiCo2O4/rGO) was first applied for the electrochemical determination of rutin. The synthesized NiCo2O4 and NiCo2O4/rGO were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) method. The sensor not only showed a satisfactory linear range (0.1-150μM) and detection limit (0...
March 1, 2017: Talanta
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