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https://www.readbyqxmd.com/read/28222433/high-glucose-induces-mouse-mesangial-cell-overproliferation-via-inhibition-of-hydrogen-sulfide-synthesis-in-a-tlr-4-dependent-manner
#1
Tao Ding, Wei Chen, Juan Li, Jiarong Ding, Xiaobin Mei, Haiyan Hu
BACKGROUND/AIMS: Overproliferation of mesangial cells was believed to play an important role in the progress of diabetic nephropathy, one of the primary complications of diabetes. Hydrogen sulfide (H2S), a well-known and pungent gas with the distinctive smell of rotten eggs, was discovered to play a protective role in diabetic nephropathy. METHODS: MTT assay was used to examine the viability of mesangial cells. Small interfering RNA was used to knock down the expression of TLR4 while specific inhibitor LY294002 to suppress the function of PI3K...
February 22, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28222258/a-simple-and-large-scale-strategy-to-prepare-flexible-graphene-tape-electrode
#2
Li Wang, Jie Yu, Yayun Zhang, Han Yang, Longfei Miao, Yonghai Song
A simple and large-scale strategy to prepare flexible graphene tape electrode (GTE) was proposed. The flexible GTE was prepared by a facile peeling method in which a piece of commercial graphite foil was firstly covered by a commercial acrylic transparent tape and then the transparent adhesive tape was quickly torn off from the graphite foil. Scanning electron microscopy results showed that some folded and wrinkled graphene layers stood up on the GTE surface to form three-dimensional (3D) porous graphene foam...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28222047/coherent-control-of-optical-absorption-and-the-energy-transfer-pathway-of-an-infrared-quantum-dot-hybridized-with-a-vo2-nanoparticle
#3
Ali Hatef, Naser Zamani, William Johnston
We systematically investigate the optical response of a semiconductor quantum dot (QD) hybridized with a vanadium dioxide nanoparticle (VO2NP) in the infrared (IR) region. The VO2NP features a semiconductor to metal phase change characteristic below and above a critical temperature that leads to an abrupt change in the particle's optical properties. This feature means that the QD-VO2NP hybrid system can support the coherent coupling of exciton-polaritons and exciton-plasmon polaritons in the semiconductor and metal phases of the VO2NP, respectively...
February 21, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28221982/guidelines-to-validate-control-of-cross-contamination-during-washing-of-fresh-cut-leafy-vegetables
#4
D Gombas, Y Luo, J Brennan, G Shergill, R Petran, R Walsh, H Hau, K Khurana, B Zomorodi, J Rosen, R Varley, K Deng
The U.S. Food and Drug Administration requires food processors to implement and validate processes that will result in significantly minimizing or preventing the occurrence of hazards that are reasonably foreseeable in food production. During production of fresh-cut leafy vegetables, microbial contamination that may be present on the product can spread throughout the production batch when the product is washed, thus increasing the risk of illnesses. The use of antimicrobials in the wash water is a critical step in preventing such water-mediated cross-contamination; however, many factors can affect antimicrobial efficacy in the production of fresh-cut leafy vegetables, and the procedures for validating this key preventive control have not been articulated...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221814/an-amperometric-glucose-sensor-integrated-into-an-insulin-delivery-cannula-in-vitro-and-in-vivo-evaluation
#5
W Kenneth Ward, Gabriel Heinrich, Matthew Breen, Sheila Benware, Nicole Vollum, Kristin Morris, Chad Knutsen, Joseph D Kowalski, Scott Campbell, Jerry Biehler, Mark S Vreeke, Scott M Vanderwerf, Jessica R Castle, Robert S Cargill
BACKGROUND: Labeling prohibits delivery of insulin at the site of subcutaneous continuous glucose monitoring (CGM). Integration of the sensing and insulin delivery functions into a single device would likely increase the usage of CGM in persons with type 1 diabetes. METHODS: To understand the nature of such interference, we measured glucose at the site of bolus insulin delivery in swine using a flexible electrode strip that was laminated to the outer wall of an insulin delivery cannula...
February 21, 2017: Diabetes Technology & Therapeutics
https://www.readbyqxmd.com/read/28221782/the-cation-%C3%AF-interaction-enables-a-halo-tag-fluorogenic-probe-for-fast-no-wash-live-cell-imaging-and-gel-free-protein-quantification
#6
Yu Liu, Kun Miao, Noah P Dunham, Hongbin Liu, Matthew Fares, Amie K Boal, Xiaosong Li, Xin Zhang
Design of fluorogenic probes for Halo-tag is highly desirable but challenging. Previous work achieved this goal by controlling the chemical switch of spirolactones upon the covalent conju-gation between Halo-tag and probes or by incorporating a 'channel dye' into the substrate bind-ing tunnel of Halo-tag. In this work, we have developed a novel class of Halo-tag fluorogenic probes that are derived from solvatochromic fluorophores. The optimal probe, harboring a ben-zothiadiazole scaffold, exhibits a 1000-fold fluorescence enhancement upon reaction with Halo-tag...
February 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28221714/photonic-devices-out-of-equilibrium-transient-memory-signal-propagation-and-sensing
#7
Thomas Heuser, Rémi Merindol, Sebastian Loescher, Aileen Klaus, Andreas Walther
Soft photonic materials are important for sensors, displays, or energy management and have become switchable under static equilibrium conditions by integration of responsive polymer features. The next step is to equip such materials with the ability for autonomously dynamic and self-regulating behavior, which would advance their functionality and application possibilities to new levels. Here, this study shows the system integration of a nonlinear, biocatalytic pH-feedback system with a pH-responsive block copolymer photonic gel, and demonstrates autonomous transient memories, remotely controlled signal propagation, and sensing...
February 21, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28221392/a-4-5-quinolimide-based-fluorescent-sensor-for-the-turn-on-detection-of-cd-2-with-live-cell-imaging
#8
Yu Zhang, Xiangfeng Guo, Mengmeng Zheng, Rui Yang, Hongming Yang, Lihua Jia, Mengmeng Yang
A 4,5-quinolimide derivative, BNA, bearing the amide-DPA receptor, was synthesized as a turn-on fluorescent sensor for Cd(2+). Under physiological conditions, BNA could distinguish Cd(2+) from Zn(2+), showing turn-on fluorescence behaviour and an increased fluorescence lifetime. BNA and Cd(2+) formed a 1 : 1 stoichiometric complex, and the detection limit was measured to be as low as 11 nM. Furthermore, BNA was utilized for fluorescence imaging of Cd(2+) in live cells. To the best of our knowledge, it is the first 4,5-quinolimide-based sensor for the detection of metal ions...
February 21, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28221201/influence-of-fraction-of-inspired-oxygen-on-noninvasive-hemoglobin-measurement-parallel-assessment-of-2-monitors
#9
Etienne Gayat, Nicolas Imbert, Ariane Roujansky, Léa Lemasle, Marc Fischler
BACKGROUND: Previous reports have brought specific attention to the relationship between oxygenation of the patient and the accuracy of noninvasive measurement of hemoglobin (Hb) using an optical sensor. This study aimed to assess prospectively the relationship between fraction of inspired oxygen (FIO2) and the bias of the measurement of Hb by the use of 2 different noninvasive monitors compared with the classic invasive technique. METHODS: Forty-four patients were included prospectively...
February 17, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28221023/polymorph-dependent-electrogenerated-chemiluminescence-of-low-dimensional-organic-semiconductor-structures-for-sensing
#10
Jianmin Gu, Yahui Gao, Jingxiao Wu, Qing Li, Aixue Li, Wei Zhang, Haiyun Dong, Bin Wen, Faming Gao, Yong Sheng Zhao
A sensitive electrogenerated chemiluminescence (ECL) sensor with organic semiconductor as active materials for detecting trace amounts of molecules have been highly desired. However, the crystal structure responses of the ECL properties of the organic semiconductor materials, that is structure-property relationship, is not clear, which limits the development of the sensitive ECL sensors. Herein, for the first time, we reported a novel concept for molecular stacking arrangement dependent electrogenerated chemiluminescence properties of organic semiconductor rubrene microstructures...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220928/malt-combined-motor-and-language-therapy-tool-for-brain-injury-patients-using-kinect
#11
Maitreyee Wairagkar, Rachel McCrindle, Holly Robson, Lotte Meteyard, Malcom Sperrin, Andy Smith, Moyra Pugh
BACKGROUND: The functional connectivity and structural proximity of elements of the language and motor systems result in frequent co-morbidity post brain injury. Although rehabilitation services are becoming increasingly multidisciplinary and "integrated", treatment for language and motor functions often occurs in isolation. Thus, behavioural therapies which promote neural reorganisation do not reflect the high intersystem connectivity of the neurologically intact brain. As such, there is a pressing need for rehabilitation tools which better reflect and target the impaired cognitive networks...
February 21, 2017: Methods of Information in Medicine
https://www.readbyqxmd.com/read/28220896/mitochondrial-dysregulation-secondary-to-endoplasmic-reticulum-stress-in-autosomal-dominant-tubulointerstitial-kidney-disease-umod-adtkd-umod
#12
Elisabeth Kemter, Thomas Fröhlich, Georg J Arnold, Eckhard Wolf, Rüdiger Wanke
'Autosomal dominant tubulointerstitial kidney disease - UMOD' (ADTKD-UMOD) is caused by impaired maturation and secretion of mutant uromodulin (UMOD) in thick ascending limb of Henle loop (TAL) cells, resulting in endoplasmic reticulum (ER) stress and unfolded protein response (UPR). To gain insight into pathophysiology, we analysed proteome profiles of TAL-enriched outer renal medulla samples from ADTKD-UMOD and control mice by quantitative LC-MS/MS. In total, 212 differentially abundant proteins were identified...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220865/symmetry-breaking-of-counter-propagating-light-in-a-nonlinear-resonator
#13
Leonardo Del Bino, Jonathan M Silver, Sarah L Stebbings, Pascal Del'Haye
Spontaneous symmetry breaking is a concept of fundamental importance in many areas of physics, underpinning such diverse phenomena as ferromagnetism, superconductivity, superfluidity and the Higgs mechanism. Here we demonstrate nonreciprocity and spontaneous symmetry breaking between counter-propagating light in dielectric microresonators. The symmetry breaking corresponds to a resonance frequency splitting that allows only one of two counter-propagating (but otherwise identical) states of light to circulate in the resonator...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220695/a-single-crystal-with-multiple-functions-of-optical-waveguide-aie-and-mechanochromism
#14
Yan Li, Zhiyong Ma, Aisen Li, Weiqing Xu, Yuechao Wang, Hong Jiang, Kang Wang, Yong Sheng Zhao, Xinru Jia
A novel single crystal PyB is produced in a high yield by a simple method of connecting a pyrene unit and a rhodamine B moiety together. PyB shows multiple functions of AIE, low-loss optical waveguiding and tricolored mechanochromism. The crucial point for fabricating such a multifunctional single crystal is selecting C=N group as a spacer, which simplifies the synthetic procedure, confines the molecular conformation to develop single crystals, and allows to dynamically observe the color variation in-situ and quantitatively analyze the effect of applied pressures...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220694/selective-fluorogenic-sensing-of-as-iii-using-aptamer-capped-nanomaterials
#15
Mar Oroval, Carmen Coll, Andrea Bernardos, María Dolores Marcos, Ramon Martinez-Manez, Dmitry G Shchukin, Felix Sancenón
Organic-inorganic hybrid nanomaterials offer extremely valuable tools for monitoring many types of analytes in in solution. Within this framework, aptamer-based nanomaterials for heavy metal detection are still very scarce. Herein, a novel sensing nanoprobe for the selective and sensitive detection of As(III) based on the combination of aptamers with mesoporous silica nanoparticles has been developed. The efficiency of the sensor is demonstrated in environmental conditions, showing a great potential in As(III) monitoring assays...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220655/ppars-in-the-central-nervous-system-roles-in-neurodegeneration-and-neuroinflammation
#16
Juan M Zolezzi, Manuel J Santos, Sussy Bastías-Candia, Claudio Pinto, Juan A Godoy, Nibaldo C Inestrosa
Over 25 years have passed since peroxisome proliferators-activated receptors (PPARs), were first described. Like other members of the nuclear receptors superfamily, PPARs have been defined as critical sensors and master regulators of cellular metabolism. Recognized as ligand-activated transcription factors, they are involved in lipid, glucose and amino acid metabolism, taking part in different cellular processes, including cellular differentiation and apoptosis, inflammatory modulation and attenuation of acute and chronic neurological damage in vivo and in vitro...
February 20, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28220534/electromyographic-assessment-of-women-s-pelvic-floor-what-is-the-best-place-for-a-superficial-sensor
#17
Eduarda Moretti, Alberto Galvão de Moura Filho, Judite Correia de Almeida, Camilla Medeiros Araujo, Andrea Lemos
AIMS: To compare the electromyographic signal of different surface electrodes and to identify the channel that presents the highest level of electromyographic activity through the mean and peak of Root Mean Square (RMS). METHODS: Thirty healthy women participated in this study. Each woman completed three collections (on different days) of electromyographic data. Three maximal voluntary contractions of the pelvic floor muscles were requested. The electromyographic signal was recorded by intravaginal probe and surface electrodes placed on the perianal region and on the region immediately below the labia majora...
February 21, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28220356/overexpression-of-line-1-retrotransposons-in-autism-brain
#18
Svitlana Shpyleva, Stepan Melnyk, Oleksandra Pavliv, Igor Pogribny, S Jill James
Long interspersed nuclear elements-1 (LINE-1 or L1) are mobile DNA sequences that are capable of duplication and insertion (retrotransposition) within the genome. Recently, retrotransposition of L1 was shown to occur within human brain leading to somatic mosaicism in hippocampus and cerebellum. Because unregulated L1 activity can promote genomic instability and mutagenesis, multiple mechanisms including epigenetic chromatin condensation have evolved to effectively repress L1 expression. Nonetheless, L1 expression has been shown to be increased in patients with Rett syndrome and schizophrenia...
February 20, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28220278/rhodamine-diaminomaleonitrile-conjugate-as-a-novel-colorimetric-fluorescent-sensor-for-recognition-of-cd-2-ion
#19
Perumal Sakthivel, Karuppannan Sekar, Gandhi Sivaraman, Subramanian Singaravadivel
Rhodamine diaminomaleonitrile linked probe (RD-1) shows highly sensitive colorimetric and selective turn-on fluorescent response to Cd(2+) over other metal ions. The fluorescence intensity and absorbance of the probe RD-1 showed a good linearity, with very low detection limits of 18.5 nm. The probe RD-1 was preliminarily applied to the determination of Cd(2+) ion in water samples from river and tap water with satisfying results. The live cell image confocal microscopy, HeLa cell demonstrated that RD-1 had low cytotoxicity with good membrane permeable property is successfully applied to fluorescence microscopic imaging for the detection of Cd(2+) ions...
February 21, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28220194/-diagnostic-ability-of-power-measurement-of-different-grip-forms-for-distal-median-nerve-lesion
#20
N Wachter, M Mentzel, G Krischak, C Häderer, J Gülke
BACKGROUND: Peripheral lesions of the median nerve cause characteristic changes of the grip function of the hand. For evaluating grip force changes, measurement by dynamometers (JAMAR dynamometer and pinch dynamometer) is of high relevance. In this study the ability of grip force measurements of different grip forms was evaluated to discriminate between a simulated median nerve lesion and healthy subjects. MATERIAL AND METHODS: In 21 healthy subjects, the grip force of power grip was measured by the JAMAR dynamometer at the second stage including measurement of force at the fingertips and the thenar by a sensor glove...
February 20, 2017: Der Unfallchirurg
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