keyword
https://read.qxmd.com/read/38536049/circ_0104652-promotes-the-proliferation-and-migration-of-ox-ldl-stimulated-vascular-smooth-muscle-cells-via-stabilizing-adamts7-and-hmgb1
#1
JOURNAL ARTICLE
Bo Bian, Heye Chen, Tianming Teng, Jinyong Huang, Xuefang Yu
BACKGROUND: Atherosclerosis (AS) stands as the primary contributor to cardiovascular disease (CVD), a pervasive global health concern. Extensive research has underscored the pivotal role of circular RNAs (circRNAs) in CVD development. However, the specific functions of numerous circRNAs in AS remain poorly understood. METHODS: Quantitative real-time PCR (RT-qPCR) analysis revealed a significant up-regulation of circ_0104652 in oxidized low-density lipoprotein (ox-LDL)-induced vascular smooth muscle cells (VSMCs)...
March 27, 2024: American Journal of Hypertension
https://read.qxmd.com/read/38535619/inhibition-of-mir-33a-5p-in-macrophage-like-cells-in-vitro-promotes-apoai-mediated-cholesterol-efflux
#2
JOURNAL ARTICLE
Olanrewaju Oladosu, Emma Chin, Christian Barksdale, Rhonda R Powell, Terri Bruce, Alexis Stamatikos
Atherosclerosis is caused by cholesterol accumulation within arteries. The intima is where atherosclerotic plaque accumulates and where lipid-laden foam cells reside. Intimal foam cells comprise of both monocyte-derived macrophages and macrophage-like cells (MLC) of vascular smooth muscle cell (VSMC) origin. Foam cells can remove cholesterol via apoAI-mediated cholesterol efflux and this process is regulated by the transporter ABCA1. The microRNA miR-33a-5p is thought to be atherogenic via silencing ABCA1 which promotes cholesterol retention and data has shown inhibiting miR-33a-5p in macrophages may be atheroprotective via enhancing apoAI-mediated cholesterol efflux...
February 28, 2024: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://read.qxmd.com/read/38534364/characterization-of-lysophospholipase-d-activity-in-mammalian-cell-membranes
#3
JOURNAL ARTICLE
Yuhuan Xie, Krishna M Ella, Terra C Gibbs, Marianne E Yohannan, Stewart M Knoepp, Pravita Balijepalli, G Patrick Meier, Kathryn E Meier
Lysophosphatidic acid (LPA) is a lipid mediator that binds to G-protein-coupled receptors, eliciting a wide variety of responses in mammalian cells. Lyso-phospholipids generated via phospholipase A2 (PLA2 ) can be converted to LPA by a lysophospholipase D (lyso-PLD). Secreted lyso-PLDs have been studied in more detail than membrane-localized lyso-PLDs. This study utilized in vitro enzyme assays with fluorescent substrates to examine LPA generation in membranes from multiple mammalian cell lines (PC12, rat pheochromocytoma; A7r5, rat vascular smooth muscle; Rat-1, rat fibroblast; PC-3, human prostate carcinoma; and SKOV-3 and OVCAR-3, human ovarian carcinoma)...
March 16, 2024: Cells
https://read.qxmd.com/read/38532120/nilotinib-induced-alterations-in-endothelial-cell-function-recapitulate-clinical-vascular-phenotypes-independent-of-abl1
#4
JOURNAL ARTICLE
Emily A Pinheiro, Jean-Marc DeKeyser, Brian Lenny, Yadav Sapkota, Paul W Burridge
Nilotinib is a highly effective treatment for chronic myeloid leukemia but has been consistently associated with the development of nilotinib-induced arterial disease (NAD) in a subset of patients. To date, which cell types mediate this effect and whether NAD results from on-target mechanisms is unknown. We utilized human induced pluripotent stem cells (hiPSCs) to generate endothelial cells and vascular smooth muscle cells for in vitro study of NAD. We found that nilotinib adversely affects endothelial proliferation and migration, in addition to increasing intracellular nitric oxide...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38532071/dysregulated-cellular-metabolism-in-atherosclerosis-mediators-and-therapeutic-opportunities
#5
REVIEW
Chad Stroope, Felix Sebastian Nettersheim, Brian Coon, Alexandra C Finney, Martin A Schwartz, Klaus Ley, Oren Rom, Arif Yurdagul
Accumulating evidence over the past decades has revealed an intricate relationship between dysregulation of cellular metabolism and the progression of atherosclerotic cardiovascular disease. However, an integrated understanding of dysregulated cellular metabolism in atherosclerotic cardiovascular disease and its potential value as a therapeutic target is missing. In this Review, we (1) summarize recent advances concerning the role of metabolic dysregulation during atherosclerosis progression in lesional cells, including endothelial cells, vascular smooth muscle cells, macrophages and T cells; (2) explore the complexity of metabolic cross-talk between these lesional cells; (3) highlight emerging technologies that promise to illuminate unknown aspects of metabolism in atherosclerosis; and (4) suggest strategies for targeting these underexplored metabolic alterations to mitigate atherosclerosis progression and stabilize rupture-prone atheromas with a potential new generation of cardiovascular therapeutics...
March 26, 2024: Nature metabolism
https://read.qxmd.com/read/38531797/knockdown-of-linc01128-downregulates-fut8-to-inhibit-oxldl-induced-vascular-smooth-muscle-cell-apoptosis-in-atherosclerosis
#6
JOURNAL ARTICLE
Guozhen Ni, Jian Xu
BACKGROUND: The apoptosis of vascular smooth muscle cells (VSMCs) contributes to the progression of atherosclerosis (AS). Long intergenic non-protein coding RNA 1128 (LINC01128) has been implicated in AS, and this study aims to uncover the role and mechanism of LINC01128 in regulating oxidized low-density lipoprotein (oxLDL)-induced VSMCs. METHODS: The position of LINC01128 in cells and its target genes were predicted using bioinformatics. The localization of LINC01128 in human VSMCs was determined through fluorescence in situ hybridization...
March 2024: Discovery Medicine
https://read.qxmd.com/read/38531794/creation-of-an-atherosclerosis-model-using-palmitic-acid-and-oleic-acid-in-the-vascular-smooth-muscle-cells-of-rats
#7
JOURNAL ARTICLE
Fan Zhang, Guangping Li, Xueyin Li, Yi Zheng, Wanting Du, Bo Zhao, Da Sun
BACKGROUND: Atherosclerosis (AS) is a chronic vascular inflammatory disease resulting from vascular endothelial injury and lipid deposition, closely linked to abnormal lipid metabolism within the body. The critical processes involved in atherosclerosis encompass lipid deposition, oxidation, metabolic disruptions, and inflammatory stimulation within the inner vessel wall. Lipid deposition emerges as a pivotal factor triggering these pathological changes, with vascular smooth muscle cells (VSMCs) playing a significant role in the development of AS...
March 2024: Discovery Medicine
https://read.qxmd.com/read/38530015/hypomethylation-of-the-lncrna-h19-promoter-accelerates-osteogenic-differentiation-of-vascular-smooth-muscle-cells-by-activating-the-erk1-2-pathways
#8
JOURNAL ARTICLE
Taoxia Wang, Meijuan Cheng, Jingjing Jin, Yaling Bai, Dongxue Zhang, Shenglei Zhang, Jinsheng Xu
OBJECTIVE: Vascular calcification is a common chronic kidney disease complication. This study aimed to investigate the function of long non-coding RNA (LncRNA) H19 in vascular calcification to explore new therapeutic strategies. METHODS: We induced osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) using β-glycerophosphate. Then, we detected the LncRNA H19 promoter methylation status and Erk1/2 pathways using methylation-specific polymerase chain reaction and western blotting, respectively...
March 2024: Journal of International Medical Research
https://read.qxmd.com/read/38529329/recent-advances-of-myotubularin-related-mtmr-protein-family-in-cardiovascular-diseases
#9
REVIEW
Jia Wang, Wei Guo, Qiang Wang, Yongjian Yang, Xiongshan Sun
Belonging to a lipid phosphatase family containing 16 members, myotubularin-related proteins (MTMRs) are widely expressed in a variety of tissues and organs. MTMRs preferentially hydrolyzes phosphatidylinositol 3-monophosphate and phosphatidylinositol (3,5) bis-phosphate to generate phosphatidylinositol and phosphatidylinositol 5-monophosphate, respectively. These phosphoinositides (PIPs) promote membrane degradation during autophagosome-lysosomal fusion and are also involved in various regulatory signal transduction...
2024: Frontiers in Cardiovascular Medicine
https://read.qxmd.com/read/38528924/a-new-oxime-synthesized-from-senecio-nutans-sch-bip-chachacoma-reduces-calcium-influx-in-the-vascular-contractile-response-in-rat-aorta
#10
JOURNAL ARTICLE
Javier Palacios, Daniel Asunción-Alvarez, Diego Aravena, Mario Chiong, Marcelo A Catalán, Claudio Parra, Fredi Cifuentes, Adrián Paredes
Senecio nutans Sch. Bip is an endemic plant commonly employed in the Andes culture to counteract the effects of mountain sickness, and its bioactive molecules could provide new drugs for treating hypertension. The purpose was to determine whether the vascular response of the plant bioactive molecules, such as (5-acetyl-6-hydroxy-2-isopropenyl-2,3-dihydrobenzofurane; Sn-I), could be improved by a simple structural modification to synthesize oximes (Ox-Sn-I). We characterized both compounds using IR and NMR spectroscopy and Heteronuclear Multiple Quantum Coherence (HMQC)...
March 20, 2024: RSC Advances
https://read.qxmd.com/read/38527985/endothelial-cell-proliferation-and-vascular-patterns-in-urothelial-carcinoma
#11
JOURNAL ARTICLE
Tiberiu Gabriel Adelmann, Amalia Raluca Ceauşu, Nela Puşa Gaje, Marius Raica
INTRODUCTION: The bladder cancer has some characteristics: the sixth most incident neoplasm in the United States, the majority of diagnosed cases in those 55 years of age and older, four times more common in man than women, a reduced five-year survival rate in case of metastatic disease. Despite the beneficial effects of the combination therapy and immunotherapy, the low response rate and drug resistance were reported. The main goal of this work was evaluation of the endothelial cell proliferation from urothelial carcinomas...
2024: Romanian Journal of Morphology and Embryology
https://read.qxmd.com/read/38526708/role-of-c-ebp-homologous-protein-in-vascular-stenosis-after-carotid-artery-injury
#12
JOURNAL ARTICLE
Lin Teng, Qin Qin, Zi-Yi Zhou, Fei Zhou, Cun-Yu Cao, Chao He, Jia-Wang Ding, Jian Yang
The study aims to explore the fluctuating expression of C/EBP Homologous Protein (CHOP) following rat carotid artery injury and its central role in vascular stenosis. Using in vivo rat carotid artery injury models and in vitro ischemia and hypoxia cell models employing human aortic endothelial cells (HAECs) and vascular smooth muscle cells (T/G HA-VSMCs), a comprehensive investigative framework was established. Histological analysis confirmed intimal hyperplasia in rat models. CHOP expression in vascular tissues was assessed using Western blot and immunohistochemical staining, and its presence in HAECs and T/G HA-VSMCs was determined through RT-PCR and Western blot...
March 25, 2024: Biochemical Genetics
https://read.qxmd.com/read/38525887/comapping-cellular-content-and-extracellular-matrix-with-hemodynamics-in-intact-arterial-tissues-using-scanning-immunofluorescent-multiphoton-microscopy
#13
JOURNAL ARTICLE
Yasutaka Tobe, Anne M Robertson, Mehdi Ramezanpour, Juan R Cebral, Simon C Watkins, Fady T Charbel, Sepideh Amin-Hanjani, Alexander K Yu, Boyle C Cheng, Henry H Woo
Deviation of blood flow from an optimal range is known to be associated with the initiation and progression of vascular pathologies. Important open questions remain about how the abnormal flow drives specific wall changes in pathologies such as cerebral aneurysms where the flow is highly heterogeneous and complex. This knowledge gap precludes the clinical use of readily available flow data to predict outcomes and improve treatment of these diseases. As both flow and the pathological wall changes are spatially heterogeneous, a crucial requirement for progress in this area is a methodology for acquiring and comapping local vascular wall biology data with local hemodynamic data...
March 25, 2024: Microscopy and Microanalysis
https://read.qxmd.com/read/38525531/optimization-of-mouse-kidney-digestion-protocols-for-single-cell-applications
#14
JOURNAL ARTICLE
Jake N Robertson, Henry Diep, Alexander Pinto, Christopher G Sobey, Grant R Drummond, Antony Vinh, Maria Jelinic
Single-cell technologies such as flow cytometry and single-cell RNA sequencing (scRNAseq) have allowed for comprehensive characterisation of the kidney cellulome. However, there is disparity in the various protocols for preparing kidney single-cell suspensions. We aimed to address this limitation by characterising kidney cellular heterogeneity using three previously published single-cell preparation protocols. Single-cell suspensions were prepared from male and female C57BL/6 kidneys using the following kidney tissue dissociation protocols: (P1) a scRNAseq protocol; (P2) a multi-tissue digestion kit from Miltenyi Biotec ; and (P3) a protocol established in our laboratory...
March 25, 2024: Physiological Genomics
https://read.qxmd.com/read/38524547/potential-actions-of-capsaicin-for-preventing-vascular-calcification-of-vascular-smooth-muscle-cells-in-vitro-and-in-vivo
#15
JOURNAL ARTICLE
Yin-Fang Yan, Yue Feng, Si-Min Wang, Fei Fang, Hong-Yan Chen, Ming-Xia Zhen, Yu-Qiang Ji, Song-Di Wu
Vascular calcification (VC) is an accurate risk factor and predictor of adverse cardiovascular events; however, there is currently no effective therapy to specifically prevent VC progression. Capsaicin (Cap) is a bioactive alkaloid isolated from Capsicum annuum L., a traditional medicinal and edible plant that is beneficial for preventing cardiovascular diseases. However, the effect of Cap on VC remains unclear. This study aimed to explore the effects and related mechanisms of Cap on aortic calcification in a mouse and on Pi-induced calcification in vascular smooth muscle cells (VSMCs)...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38524279/nf-kb-affects-migration-of-vascular-smooth-muscle-cells-after-treatment-with-heparin-and-ibrutinib
#16
JOURNAL ARTICLE
Nafiseh Shokri, Ghasem Ghasempour, Ali Akbar Soleimani, Mohammad Elahimanesh, Mohammad Najafi
The migration of vascular smooth muscle cells (VSMCs) is one of the most important events in the remodeling of atherosclerosis plaque. The aim of study was to investigate the role of Heparin in the VSMC migration and its association with the NF-kB, collagen 1 and collagen 3 expression levels. Moreover, the incorporation of Heparin was studied in the VSMC cultures including Betulinic acid and Ibrutinib. Twelve cell groups were cultured and treated with the Heparin, Betulinic acid and Ibrutinib based on the viability and toxicity in 24-h and 48-h periods...
July 2024: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/38524234/circulating-mir-129-3p-in-combination-with-clinical-factors-predicts-vascular-calcification-in-hemodialysis-patients
#17
JOURNAL ARTICLE
Jingjing Jin, Meijuan Cheng, Xueying Wu, Haixia Zhang, Dongxue Zhang, Xiangnan Liang, Yuetong Qian, Liping Guo, Shenglei Zhang, Yaling Bai, Jinsheng Xu
BACKGROUND: Vascular calcification (VC) commonly occurs and seriously increases the risk of cardiovascular events and mortality in patients with hemodialysis. For optimizing individual management, we will develop a diagnostic multivariable prediction model for evaluating the probability of VC. METHODS: The study was conducted in four steps. First, identification of miRNAs regulating osteogenic differentiation of vascular smooth muscle cells (VSMCs) in calcified condition...
March 2024: Clinical Kidney Journal
https://read.qxmd.com/read/38523689/fto-stabilizes-mis12-to-inhibit-vascular-smooth-muscle-cell-senescence-in-atherosclerotic-plaque
#18
JOURNAL ARTICLE
Jingzhao Sun, Mengqi Wang, Fengming Jia, Jiantao Song, Jinlin Ren, Bo Hu
PURPOSE: Atherosclerosis is the main cause of atherosclerotic cardiovascular disease (CVD). Here, we aimed to uncover the role and mechanisms of fat mass and obesity-associated genes (FTO) in the regulation of vascular smooth muscle cell (VSMC) senescence in atherosclerotic plaques. METHODS: ApoE-/- mice fed a high-fat diet (HFD) were used to establish an atherosclerotic animal model. Immunohistochemistry, and the staining of hematoxylin-eosin, Oil Red O, Sirius red, and Masson were performed to confirm the role of FTO in atherosclerosis in vivo...
2024: Journal of Inflammation Research
https://read.qxmd.com/read/38522069/mafb-in-macrophages-regulates-cold-induced-neuronal-density-in-brown-adipose-tissue
#19
JOURNAL ARTICLE
Manoj Kumar Yadav, Megumi Ishida, Natalia Gogoleva, Ching-Wei Liao, Filiani Natalia Salim, Maho Kanai, Akihiro Kuno, Takuto Hayashi, Zeynab Javanfekr Shahri, Kaushalya Kulathunga, Omar Samir, Wenxin Lyu, Olivia Olivia, Evaristus C Mbanefo, Satoru Takahashi, Michito Hamada
Transcription factor MAFB regulates various homeostatic functions of macrophages. This study explores the role of MAFB in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f ::LysM-Cre) mice. We find that Mafb deficiency in macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density in BAT under cold conditions. This phenotype features a proinflammatory environment that is characterized by macrophage/granulocyte accumulation, increases in interleukin-6 (IL-6) production, and IL-6 trans-signaling, which lead to decreases in nerve growth factor (NGF) expression and reduction in SN density in BAT...
March 22, 2024: Cell Reports
https://read.qxmd.com/read/38519881/vascular-smooth-muscle-cells-in-response-to-cholesterol-crystals-modulates-inflammatory-cytokines-release-and-promotes-neutrophil-extracellular-trap-formation
#20
JOURNAL ARTICLE
Jishamol Thazhathveettil, Ashok Kumar Kumawat, Isak Demirel, Allan Sirsjö, Geena Varghese Paramel
BACKGROUND: The formation and accumulation of cholesterol crystals (CC) at the lesion site is a hallmark of atherosclerosis. Although studies have shown the importance of vascular smooth muscle cells (VSMCs) in the disease atherosclerosis, little is known about the molecular mechanism behind the uptake of CC in VSMCs and their role in modulating immune response. METHODS: Human aortic smooth muscle cells were cultured and treated with CC. CC uptake and CC mediated signaling pathway and protein induction were studied using flow cytometry, confocal microscopy, western blot and Olink proteomics...
March 22, 2024: Molecular Medicine
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