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https://www.readbyqxmd.com/read/27916732/ghrelin-improves-vascular-autophagy-in-rats-with-vascular-calcification
#1
Mingming Xu, Lin Liu, Chenfang Song, Wei Chen, Shuyan Gui
BACKGROUNDS: This study aimed to investigate whether ghrelin ameliorated vascular calcification (VC) through improving autophagy. METHODS: VC model was induced by nicotine plus vitamin D3 in rats and β-glycerophosphate in vascular smooth muscle cell (VSMC). Calcium deposition was detected by von Kossa staining or alizarin red S staining. ALP activity was also detected. Western blot was used to assess the protein expression. RESULTS: Ghrelin treatment attenuated the elevation of calcium deposition and ALP activity in VC model both in vivo and in vitro...
December 1, 2016: Life Sciences
https://www.readbyqxmd.com/read/27911586/mirna-181a-b-regulates-phenotypes-of-vessel-smooth-muscle-cells-through-serum-response-factor
#2
Xiaoxing Wei, Xue Hou, Jianhua Li, Yongnian Liu
The phenotypic modulation of vessel smooth muscle cells (VSMCs) plays a crucial role in the physiological and pathological conditions of vasculature in response to local environmental changes. The phenotypic transition of VSMCs is largely modulated by the serum response factor (SRF). MiR-181a and miR-181b are members of the well-studied miR-181 family and both have complementary sequence in the 3' untranslated region (UTR) of SRF gene. In this article, evidence insinuates that miR-181a/b was involved in VSMCs differentiation through upregulating synthetic marker genes and downregulating contractile ones, respectively...
December 2, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27910812/inflammatory-markers-associated-with-abdominal-aortic-aneurysm
#3
Sarah Lindberg, Moncef Zarrouk, Jan Holst, Anders Gottsäter
PURPOSE: Inflammation with leukocytic infiltration, degradation of extracellular matrix (ECM), and depletion of vascular smooth muscle cells (VSMC) are pathological hallmarks of abdominal aortic aneurysm (AAA). The aim of this study was to further evaluate relationships between AAA and inflammatory biomarkers, interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), endothelin-1 (ET-1) and soluble urokinase-type plasminogen activator receptor (suPAR), by comparing levels in 65-year-old men with and without AAA at ultrasound screening...
September 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/27910161/mir-29a-regulates-vascular-neointimal-hyperplasia-by-targeting-yy1
#4
Yu Zhang, Yong Hui Li, Chao Liu, Chan-Juan Nie, Xin-Hua Zhang, Cui-Ying Zheng, Wen Jiang, Wei-Na Yin, Ming-Hui Ren, Yu-Xin Jin, Shu-Feng Liu, Bin Zheng, Jin-Kun Wen
OBJECTIVES: The formation of vascular neointima is mainly related to impairment of the vascular endothelial barrier and abnormal proliferation and migration of smooth muscle cells. The objective of this study was to investigate whether miR-29a exerts any promoting effect on the vascular neointimal hyperplasia and if so, its mechanism. MATERIALS AND METHODS: RT-qPCR was performed to determine expression of miR-29a in vascular smooth muscle cells (VSMC) and vascular neointimal hyperplasia...
December 2, 2016: Cell Proliferation
https://www.readbyqxmd.com/read/27905925/expression-of-insulin-receptor-ir-a-and-b-isoforms-igf-ir-and-ir-igf-ir-hybrid-receptors-in-vascular-smooth-muscle-cells-and-their-role-in-cell-migration-in-atherosclerosis
#5
N Beneit, C E Fernández-García, J L Martín-Ventura, L Perdomo, Ó Escribano, J B Michel, G García-Gómez, S Fernández, S Díaz-Castroverde, J Egido, A Gómez-Hernández, M Benito
BACKGROUND: Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) is a major contributor to the development of atherosclerotic process. In a previous work, we demonstrated that the insulin receptor isoform A (IRA) and its association with the insulin-like growth factor-I receptor (IGF-IR) confer a proliferative advantage to VSMCs. However, the role of IR and IGF-IR in VSMC migration remains poorly understood. METHODS: Wound healing assays were performed in VSMCs bearing IR (IRLoxP(+/+) VSMCs), or not (IR(-/-) VSMCs), expressing IRA (IRA VSMCs) or expressing IRB (IRB VSMCs)...
December 1, 2016: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/27904672/t3-inhibits-the-calcification-of-vascular-smooth-muscle-cells-and-the-potential-mechanism
#6
Xiaodan Chang, Baohong Zhang, Li Lihua, Zhichun Feng
OBJECTIVE: This study aimed to investigate the potential molecular mechanism underlying the T3 induced vascular calcification and phenotype transformation of vascular smooth muscle cells (VSMCs). METHODS: Rat thoracic aortic smooth muscle cells (A7r5) were cultured in vitro and randomly assigned into normal control group, calcification group, T3 group and inhibitor group. RESULTS: When compared with normal control group, the osteocalcin content, ALP activity, Osterix and Runx2 mRNA expression and OPN protein expression increased significantly (P<0...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27903395/-role-of-cytokine-matrix-metalloproteinase-axis-on-promoting-vascular-neointima-hyperplasia-in-mice
#7
Y Liu, W H Ning, X H Shen, D L Guo, L Guo
Objective: To observe the effects of tumor necrosis factor-α (TNF-α) and platelet derived growth factor (PDGF) on vascular neointimal hyperplasia on matrix metalloproteinase 9/2 gene knockout (MMP9/2(-/-)) mice and explore related mechanisms. Methods: Mice of control group, MMP9(-/-) group, MMP2(-/-) group and MMP9/2(-/-) group were studied. Femoral artery was injured by transluminal wire, the mRNA expression levels of TNF-α and PDGF on femoral artery were detected by RT-PCR; the protein expression of MMP9 and MMP2 were assessed by Western blot on day 0, 1, 3, 7, 14 and 28 post injury...
November 24, 2016: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/27888098/identification-of-novel-peptide-motifs-in-the-serpin-maspin-that-affect-vascular-smooth-muscle-cell-function
#8
S E Jenkinson, L J Brown, J Ombor, J A Milburn, T Smulders-Srinivasan, S Veuger, D R Edwards, R Bass
Maspin is a non-inhibitory member of the serpin family that affects cell behaviours related to migration and survival. We have previously shown that peptides of the isolated G α-helix (G-helix) domain of maspin show bioactivity. Migration, invasion, adhesion and proliferation of vascular smooth muscle cells (VSMC) are important processes that contribute to the build-up of atherosclerotic plaques. Here we report the use of functional assays of these behaviours to investigate whether other maspin-derived peptides impact directly on VSMC; focusing on potential anti-atherogenic properties...
November 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27886226/functional-improvement-and-regression-of-medial-hypertrophy-in-the-remodeled-pulmonary-artery-after-correction-of-systemic-left-to-right-shunt
#9
Chih-Hsin Hsu, Jun-Neng Roan, Jyh-Hong Chen, Chen-Fuh Lam
The presence of systemic left-to-right shunt and increased pulmonary blood flow can result in right heart failure and pulmonary arteriopathy. Correction of left-to-right shunt has been shown to improve cardiac function and physical performance. However, the cardiopulmonary remodeling processes following cessation of left-to-right shunt have yet to be reported. In this experimental study, excessive pulmonary flow was restored through ligation of the aortocaval fistula in rats with flow-induced pulmonary hypertension...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881420/elongation-of-long-chain-fatty-acid-family-member-6-elovl6-driven-fatty-acid-metabolism-regulates-vascular-smooth-muscle-cell-phenotype-through-amp-activated-protein-kinase-kr%C3%A3-ppel-like-factor-4-ampk-klf4-signaling
#10
Hiroaki Sunaga, Hiroki Matsui, Saki Anjo, Mas Risky A A Syamsunarno, Norimichi Koitabashi, Tatsuya Iso, Takashi Matsuzaka, Hitoshi Shimano, Tomoyuki Yokoyama, Masahiko Kurabayashi
BACKGROUND: Fatty acids constitute the critical components of cell structure and function, and dysregulation of fatty acid composition may exert diverging vascular effects including proliferation, migration, and differentiation of vascular smooth muscle cells (VSMCs). However, direct evidence for this hypothesis has been lacking. We investigated the role of elongation of long-chain fatty acid member 6 (Elovl6), a rate-limiting enzyme catalyzing the elongation of saturated and monounsaturated long-chain fatty acid, in the regulation of phenotypic switching of VSMC...
November 23, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27879253/the-yin-yang-dynamics-of-dna-methylation-is-the-key-regulator-for-smooth-muscle-cell-phenotype-switch-and-vascular-remodeling
#11
Jianhui Zhuang, Peipei Luan, Hailing Li, Kai Wang, Pei Zhang, Yawei Xu, Wenhui Peng
OBJECTIVE: DNA methylation plays an important role in chronic diseases such as atherosclerosis, yet the mechanisms are poorly understood. The objective of our study is to indicate the regulatory mechanisms of DNA methylation in vascular smooth muscle cells (VSMCs) and its roles in atherosclerosis. APPROACH AND RESULTS: In ApoE(-/-) mice fed a Western diet, DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine, significantly attenuated atherosclerotic lesions (20...
November 22, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/27876618/tlr4-nf-%C3%AE%C2%BAb-ceramide-signaling-contributes-to-ox-ldl-induced-calcification-of-human-vascular-smooth-muscle-cells
#12
Yan Song, Menglin Hou, Zhenlin Li, Chufan Luo, Jing-Song Ou, Huimin Yu, Jianyun Yan, Lihe Lu
Vascular calcification is a major feature of advanced atherosclerosis and highly associated with cardiovascular diseases. Oxidized low density lipoprotein (Ox-LDL) has been recognized as a critical risk factor for atherosclerosis and osteogenic differentiation of vascular smooth muscle cells (VSMCs). Previous studies have demonstrated that toll like receptor 4 (TLR4) is highly expressed in atherosclerotic lesions and participates in the progression of atherosclerosis. However, the role of TLR4 in vascular calcification remains unknown...
November 19, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27873518/mir-379-inhibits-cell-proliferation-invasion-and-migration-of-vascular-smooth-muscle-cells-by-targeting-insulin-like-factor-1
#13
Kai Li, Yong Wang, Anji Zhang, Baixue Liu, Li Jia
PURPOSE: MicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof. MATERIALS AND METHODS: MicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively...
January 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/27868196/differential-regulation-of-blood-flow-induced-neovascularisation-and-mural-cell-recruitment-by-vegf-and-angiopoietin-signalling
#14
Oliver A Stone, James G Carter, P Charles Lin, Ewa Paleolog, Maria J C Machado, David O Bates
Signalling through VEGF receptors and the Tie2 receptor by angiopoietins is required in combination with blood flow for the formation of a functional vascular network. We tested the hypothesis that VEGF and Ang1 contribute differentially to neovascularization induced by nitric oxide (NO) mediated vasodilatation, by comparing the phenotype of new microvessels in the mesentery during induction of vascular remodelling by over-expression of endothelial nitric oxide synthase (eNOS) in the fat pad of the adult rat mesentery during inhibition of Angiopoietin signalling with soluble Tie2 and VEGF signalling with sFlt1...
November 21, 2016: Journal of Physiology
https://www.readbyqxmd.com/read/27866196/down-regulation-of-protein-kinase-c-%C3%AE%C2%B5-by-prolonged-incubation-with-pma-inhibits-the-proliferation-of-vascular-smooth-muscle-cells
#15
Huixuan Zhou, Yan Wang, Quanhong Zhou, Bin Wu, Aizhong Wang, Wei Jiang, Li Wang
BACKGROUND/AIMS: Phorbol myristate acetate (PMA) exerts a pleiotropic effect on the growth and differentiation of various cells. Protein kinase Cs (PKCs) plays a central role in mediating the effects of PMA on cells. The present study investigated whether the down-regulation of protein kinase C-ε (PKC-ε) is involved in the inhibition of vascular smooth muscle cell (VSMC) proliferation caused by prolonged PMA incubation. METHODS: Using cell counting, Cell Counting Kit-8 (CCK-8) and EdU incorporation assay on VSMCs, we evaluated the inhibitory effects of prolonged incubation of PMA, of lentiviruses carrying the short-hairpin RNAs (shRNA) of PKC-ε and of the PKC-ε inhibitor peptide on the proliferation and viability of cells...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27857867/dna-methylation-signature-of-post-injury-neointimal-cells-during-vascular-remodeling-in-the-rat-balloon-injury-model
#16
Jendai Richards, Henry Ato Ogoe, Wenzhi Li, Oguljahan Babayewa, Wei Xu, Tameka Bythwood, Minerva Garcia-Barrios, Li Ma, Qing Song
Vascular smooth muscle cell (VSMC) accumulation in the neointimal is a common feature in vascular diseases such as atherosclerosis, transplant arteriosclerosis and restenosis. In this study, we isolated the neointimal cells and uninjured residential vascular smooth muscle cells by laser micro dissection and carried out single-cell whole-genome methylation sequencing. We also sequenced the bisulfite converted genome of circulating bone-marrow-derived cells such as peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC)...
July 2016: Molecular Biology (Los Angeles, Calif.)
https://www.readbyqxmd.com/read/27854297/prelamin-a-accumulation-attenuates-rac1-activity-and-increases-the-intrinsic-migrational-persistence-of-aged-vascular-smooth-muscle-cells
#17
Lauren J Porter, Mark R Holt, Daniel Soong, Catherine M Shanahan, Derek T Warren
Vascular smooth muscle cell (VSMC) motility is essential during both physiological and pathological vessel remodeling. Although ageing has emerged as a major risk factor in the development of cardiovascular disease, our understanding of the impact of ageing on VSMC motility remains limited. Prelamin A accumulation is known to drive VSMC ageing and we show that presenescent VSMCs, that have accumulated prelamin A, display increased focal adhesion dynamics, augmented migrational velocity/persistence and attenuated Rac1 activity...
November 15, 2016: Cells
https://www.readbyqxmd.com/read/27847551/caspase-3-deletion-promotes-necrosis-in-atherosclerotic-plaques-of-apoe-knockout-mice
#18
Mandy O J Grootaert, Dorien M Schrijvers, Marthe Hermans, Viviane O Van Hoof, Guido R Y De Meyer, Wim Martinet
Apoptosis of macrophages and vascular smooth muscle cells (VSMCs) in advanced atherosclerotic plaques contributes to plaque progression and instability. Caspase-3, a key executioner protease in the apoptotic pathway, has been identified in human and mouse atherosclerotic plaques but its role in atherogenesis is not fully explored. We therefore investigated the impact of caspase-3 deletion on atherosclerosis by crossbreeding caspase-3 knockout (Casp3(-/-)) mice with apolipoprotein E knockout (ApoE(-/-)) mice...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27833146/activation-of-protein-kinase-g-pkg-reduces-neointimal-hyperplasia-inhibits-platelet-aggregation-and-facilitates-re-endothelialization
#19
Ju-Young Kim, Han-Mo Yang, Joo-Eun Lee, Baek-Kyung Kim, Sooryeonhwa Jin, Jaewon Lee, Kyung-Woo Park, Hyun-Jai Cho, Yoo-Wook Kwon, Hae-Young Lee, Hyun-Jae Kang, Byung-Hee Oh, Young-Bae Park, Hyo-Soo Kim
In spite of its great success in reducing restenosis, drug-eluting stent (DES) has unfavorable aspects such as stent thrombosis and delayed re-endothelialization. We examined the effects of PKG activation by Exisulind on neointimal formation, platelet aggregation, and re-endothelialization. Exisulind significantly reduced VSMCs viability, cell cycle progression, migration, and neointimal hyperplasia after vascular injury in rat carotid arteries. Interestingly, in contrast to the effect on VSMC viability, Exisulind did not reduce the viability of endothelial cells...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27830723/inhibition-of-epac1-suppresses-mitochondrial-fission-and-reduces-neointima-formation-induced-by-vascular-injury
#20
Hui Wang, William G Robichaux, Ziqing Wang, Fang C Mei, Ming Cai, Guangwei Du, Ju Chen, Xiaodong Cheng
Vascular smooth muscle cell (VSMC) activation in response to injury plays an important role in the development of vascular proliferative diseases, including restenosis and atherosclerosis. The aims of this study were to ascertain the physiological functions of exchange proteins directly activated by cAMP isoform 1 (Epac1) in VSMC and to evaluate the potential of Epac1 as therapeutic targets for neointima formation during vascular remodeling. In a mouse carotid artery ligation model, genetic knockdown of the Epac1 gene led to a significant reduction in neointima obstruction in response to vascular injury...
November 10, 2016: Scientific Reports
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