keyword
MENU ▼
Read by QxMD icon Read
search

Vsmc

keyword
https://www.readbyqxmd.com/read/28740544/interleukin-32%C3%AE-inhibits-endothelial-inflammation-vascular-smooth-muscle-cell-activation-and-atherosclerosis-by-upregulating-timp3-and-reck-through-suppressing-microrna-205-biogenesis
#1
Dong Ju Son, Yu Yeon Jung, Young Sik Seo, Heonyong Park, Dong Hun Lee, Sanghyeon Kim, Yoon-Seok Roh, Sang Bae Han, Do Young Yoon, Jin Tae Hong
Interleukin-32 (IL-32) is a multifaceted cytokine that promotes inflammation and regulates vascular endothelial cell behavior. Although some IL-32 isoforms have been reported to contribute to vascular inflammation and atherosclerosis, the functional role of IL-32α in vascular inflammation and atherogenesis has not been studied. Methods: IL-32α function was assessed in cells with transient IL-32α overexpression or treated with recombinant human IL-32α by western blotting and mRNA expression analysis. Vascular smooth muscle cell (VSMC) proliferation and migration was examined by BrdU incorporation and wound healing assays, respectively...
2017: Theranostics
https://www.readbyqxmd.com/read/28735290/membrane-tethered-metalloproteinase-expressed-by-vascular-smooth-muscle-cells-limits-the-progression-of-proliferative-atherosclerotic-lesions
#2
Richard H Barnes, Takeshi Akama, Miina K Öhman, Moon-Sook Woo, Julian Bahr, Stephen J Weiss, Daniel T Eitzman, Tae-Hwa Chun
BACKGROUND: The MMP (matrix metalloproteinase) family plays diverse and critical roles in directing vascular wall remodeling in atherosclerosis. Unlike secreted-type MMPs, a member of the membrane-type MMP family, MT1-MMP (membrane-type 1 MMP; MMP14), mediates pericellular extracellular matrix degradation that is indispensable for maintaining physiological extracellular matrix homeostasis. However, given the premature mortality exhibited by MT1-MMP-null mice, the potential role of the proteinase in atherogenesis remains elusive...
July 22, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28713961/adipose-differentiation%C3%A2-related-protein-knockdown-inhibits-vascular-smooth-muscle-cell-proliferation-and-migration-and-attenuates-neointima-formation
#3
Haomin Zhao, Tao Han, Xin Hong, Dajun Sun
Vascular smooth muscle cells (VSMCs) have an important role in atherosclerosis development. Evidence has demonstrated that adipose differentiation‑related protein (ADRP) is associated with foam cell formation and atherosclerosis progression. However, to the best of our knowledge, no previous studies have investigated the role of ADRP knockdown in platelet‑derived growth factor (PDGF)‑stimulated proliferation and migration of VSMCs in vitro. Furthermore, the effect of ADRP knockdown on neointima formation in vivo remains unclear...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28711598/inos-derived-peroxynitrite-mediates-high-glucose-induced-inflammatory-gene-expression-in-vascular-smooth-muscle-cells-through-promoting-klf5-expression-and-nitration
#4
Man-Li Zhang, Bin Zheng, Fei Tong, Zhan Yang, Zhi-Bo Wang, Bao-Ming Yang, Yan Sun, Xin-Hua Zhang, Yi-Lin Zhao, Jin-Kun Wen
Inducible NO synthase (iNOS) expression and peroxynitrite formation are significantly increased in diabetic vascular tissues. Transcription factor KLF5 activates iNOS gene transcription and is involved in vascular inflammatory injury and remodeling. However, mutual regulation between KLF5, iNOS and peroxynitrite in diabetic vascular inflammation, as well as the underlying mechanisms, remain largely unknown. In this study, we found a marked increase in KLF5 and iNOS expression in vascular smooth muscle cells (VSMC) of diabetic patients...
July 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28706947/p21-activated-kinase-4-promotes-intimal-hyperplasia-and-vascular-smooth-muscle-cells-proliferation-during-superficial-femoral-artery-restenosis-after-angioplasty
#5
Liangxi Yuan, Xianli Duan, Jian Dong, Qingsheng Lu, Jian Zhou, Zhiqing Zhao, Junmin Bao, Zaiping Jing
The aim of this study is to explore the function of p21-activated kinase 4 (PAK4) in intimal hyperplasia (IH) and vascular smooth muscle cells (VSMCs) proliferation. We choose vascular samples from patients undergoing angioplasty in superficial femoral artery (SFA) as the experimental group and vascular samples from donors without clinical SFA restenosis as the control group, respectively. We draw from the results that both levels of mRNA and protein of PAK4 in the experimental group increased dramatically compared with the control group...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28706886/the-effects-of-indoxyl-sulfate-induced-endothelial-microparticles-on-neointimal-hyperplasia-formation-in-an-ex-vivo-model
#6
Jung-Hwa Ryu, HeeJung Park, Seung-Jung Kim
PURPOSE: Neointimal hyperplasia (NH) is considered to be one of the main causes of vascular access occlusion in patients receiving hemodialysis. Endothelial injury and TGF-β-mediated proliferation of vascular smooth muscle cells (VSMCs) induce NH. Endothelial microparticles (EMPs) are also increased by endothelial injury. We aimed to investigate the effects of EMPs and TGF-β expression on VSMC proliferation and their contributions to NH formation in an ex vivo model. METHODS: EMPs were collected from the culture media of human umbilical vein endothelial cells treated with indoxyl sulfate (IS, 250 µg/mL) after ultracentrifugation at 100,000 × g...
July 2017: Annals of Surgical Treatment and Research
https://www.readbyqxmd.com/read/28704617/resveratrol-prevents-angiotensin-ii-induced-hypertrophy-of-vascular-smooth-muscle-cells-through-the-transactivation-of-growth-factor-receptors
#7
Ekhtear Hossain, Madhu B Anand-Srivastava
We previously showed that the augmented levels of endogenous angiotensin II (Ang II) contribute to vascular smooth muscle cell (VSMC) hypertrophy through the transactivation of growth factor receptors in spontaneously hypertensive rats. Resveratrol (RV), a polyphenolic component of red wine, has also been shown to attenuate Ang II-evoked VSMC hypertrophy, however, the molecular mechanism mediating this response is obscure. The present study is therefore undertaken to examine if RV could prevent Ang II-induced VSMC hypertrophy through the transactivation of growth factor receptor and associated signaling pathways...
July 13, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28699690/microrna-let-7g-suppresses-pdgf-induced-conversion-of-vascular-smooth-muscle-cell-into-the-synthetic-phenotype
#8
Tzu-Ming Wang, Ku-Chung Chen, Po-Yuan Hsu, Hsiu-Fen Lin, Yung-Song Wang, Chien-Yuan Chen, Yi-Chu Liao, Suh-Hang H Juo
Platelet-derived growth factor (PDGF) can promote vascular smooth muscle cells (VSMCs) to switch from the quiescent contractile phenotype to synthetic phenotype, which contributes to atherosclerosis. We aimed to investigate the role of microRNA let-7g in phenotypic switching. Bioinformatics prediction was used to find let-7g target genes in the PDGF/mitogen-activated protein kinase kinase kinase 1 (MEKK1)/extracellular signal-regulated kinase (ERK)/Krüppel-like factor-4 (KLF4) signalling pathway that affects VSMC phenotypic switching...
July 12, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28698564/homer-binds-to-orai1-and-trpc-channels-in-the-neointima-and-regulates-vascular-smooth-muscle-cell-migration-and-proliferation
#9
Shuping Jia, Miguel Rodriguez, Arthur G Williams, Joseph P Yuan
The molecular components of store-operated Ca(2+) influx channels (SOCs) in proliferative and migratory vascular smooth muscle cells (VSMCs) are quite intricate with many channels contributing to SOCs. They include the Ca(2+)-selective Orai1 and members of the transient receptor potential canonical (TRPC) channels, which are activated by the endoplasmic reticulum Ca(2+) sensor STIM1. The scaffolding protein Homer assembles SOC complexes, but its role in VSMCs is not well understood. Here, we asked whether these SOC components and Homer1 are present in the same complex in VSMCs and how Homer1 contributes to VSMC SOCs, proliferation, and migration leading to neointima formation...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28698179/a-novel-regulatory-mechanism-of-smooth-muscle-%C3%AE-actin-expression-by-nrg-1-circacta2-mir-548f-5p-axis
#10
Yan Sun, Zhan Yang, Bin Zheng, Xin-Hua Zhang, Man-Li Zhang, Xue-Shan Zhao, Hong-Ye Zhao, Toru Suzuki, Jin-Kun Wen
Rationale: Neuregulin-1 (NRG-1) includes an extracellular EGF-like domain and an intracellular domain (NRG-1-ICD). In response to transforming growth factor (TGF)-β1,its cleavage by proteolytic enzymes releases a bioactive fragment, which suppresses the vascular smooth muscle cell (VSMC) proliferation by activating ErbB receptor. However, NRG-1-ICD function in VSMCs remains unknown. Objective: Here, we characterize the function of NRG-1-ICD and underlying mechanisms in VSMCs. Methods and Results: Immunofluorescence staining, Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) showed that NRG-1 was expressed in rat, mouse and human VSMCs and was upregulated and cleaved in response to TGF-β1...
July 11, 2017: Circulation Research
https://www.readbyqxmd.com/read/28694192/laminar-shear-stress-suppresses-vascular-smooth-muscle-cell-proliferation-through-nitric-oxide-ampk-pathway
#11
Sun Ae Kim, Jin Young Sung, Chang Hoon Woo, Hyoung Chul Choi
In healthy condition, vascular smooth muscle cells (VSMCs) are not directly exposed to shear stresses, because they are shielded by endothelial cell (EC) layer that lines blood vessels. After injury to EC layer caused by rupture of atherosclerotic lesions or invasive techniques such as angioplasty, VSMCs are directly exposed to blood flow which modulate molecular signaling and function. In endothelium, exposure to fluid shear stress has been reported to induce AMP-activated protein kinase (AMPK) phosphorylation and nitric oxide (NO) production...
July 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28693920/s100b-promotes-injury-induced-vascular-remodeling-through-modulating-smooth-muscle-phenotype
#12
Teng Cao, Lei Zhang, Ling-Ling Yao, Fei Zheng, Lu Wang, Jian-Ye Yang, Ling-Yun Guo, Xing-Yuan Li, Yu-Wen Yan, Ya-Mu Pan, Miao Jiang, Long Chen, Jun-Ming Tang, Shi-You Chen, Jia-NingWang
S100B is a biomarker of nervous system injury, but it is unknown if it is also involved in vascular injury. In the present study, we investigated S100B function in vascular remodeling following injury. Balloon injury in rat carotid artery progressively induced neointima formation while increasing S100B expression in both neointimal vascular smooth muscle (VSMC) and serum along with an induction of proliferating cell nuclear antigen (PCNA). Knockdown of S100B by its shRNA delivered by adenoviral transduction attenuated the PCNA expression and neointimal hyperplasia in vivo and suppressed PDGF-BB-induced VSMC proliferation and migration in vitro...
July 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28692698/nitric-oxide-attenuates-overexpression-of-gi%C3%AE-proteins-in-vascular-smooth-muscle-cells-from-shr-role-of-ros-and-ros-mediated-signaling
#13
Oli Sarkar, Yuan Li, Madhu B Anand-Srivastava
Vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit decreased levels of nitric oxide (NO) that may be responsible for the overexpression of Giα proteins that has been shown as a contributing factor for the pathogenesis of hypertension in SHR. The present study was undertaken to investigate if increasing the intracellular levels of NO by NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP) could attenuate the enhanced expression of Giα proteins in VSMC from SHR and explore the underlying mechanisms responsible for this response...
2017: PloS One
https://www.readbyqxmd.com/read/28672961/salidroside-inhibits-high-glucose-induced-proliferation-of-vascular-smooth-muscle-cells-via-inhibiting-mitochondrial-fission-and-oxidative-stress
#14
Xinyu Zhuang, Alimujiang Maimaitijiang, Yong Li, Haiming Shi, Xiaofei Jiang
The mitochondria are highly dynamic organelles, carefully maintaining network homeostasis by regulating mitochondrial fusion and fission. Mitochondrial dynamics are involved in the regulation of a variety of pathophysiological processes, including cell proliferation. Oxidative stress serves an important role in the remodeling of arterial vascular tissue in diabetic patients by affecting the proliferation of vascular smooth muscle cells (VSMCs). Salidroside is the primary active component of Rhodiola rosea and has been demonstrated to be an antioxidant with cardio- and vascular-protective effects, in addition to improving glucose metabolism...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28672917/downregulation-of-p16-promotes-cigarette-smoke-extract-induced-vascular-smooth-muscle-cell-proliferation-via-preventing-g1-s-phase-transition
#15
Tao Guo, Xiangping Chai, Qiming Liu, Wen Peng, Zhenyu Peng, Yuzhong Cai
The proliferation of vascular smooth muscle cells (VSMCs) serves an important role in cigarette smoking-associated vascular diseases; however, the underlying mechanisms responsible for this remain unclear. The aim of the present study was to elucidate the role of P16 in cigarette smoke extract (CSE)-induced VSMC proliferation and the underlying mechanism responsible. Human aortic smooth muscle cells (HAOSMCs) were exposed to CSE, and an MTT assay and flow cytometry were performed to evaluate cell proliferation and cell cycle distribution...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28667053/microrna-124-control-human-vascular-smooth-muscle-cell-phenotypic-switch-via-sp1
#16
Yangfeng Tang, Shangyi Yu, Yang Liu, Jiajun Zhang, Lin Han, Zhiyun Xu
Phenotypic switch of vascular smooth muscle cells (VSMCs) plays an important role in the pathogenesis of atherosclerosis and aortic dissection. However, the mechanisms of phenotypic modulation are still unclear. MicroRNAs have emerged as important regulators of VSMC function. We recently found that MicroRNA-124(miR-124) was down-regulated in proliferative vascular diseases. The purpose of this study was to investigate the critical role played by the aberrant expression of miR-124 in human aortic VSMC phenotypic switch and to unravel the signal transduction pathways...
June 30, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28659610/upregulation-of-arylsulfatase-b-in-carotid-atherosclerosis-is-associated-with-symptoms-of-cerebral-embolization
#17
Erik Biros, Corey S Moran, Jane Maguire, Elizabeth Holliday, Christopher Levi, Jonathan Golledge
The aim of this study was to identify genes for which the expression within carotid atherosclerosis was reproducibly associated with the symptoms of cerebral embolization. Two publically available microarray datasets E-MEXP-2257 and GSE21545 were analysed using GeneSpring 11.5. The two datasets utilized a total of 22 and 126 carotid atherosclerosis samples, obtained from patients with and without symptoms of cerebral embolization, respectively. To assess whether the findings were reproducible we analysed carotid atherosclerosis samples from another 8 patients with and 7 patients without symptoms of cerebral embolization using real-time PCR...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28659168/gene-expression-profiles-and-signaling-mechanisms-in-%C3%AE-2b-adrenoceptor-evoked-proliferation-of-vascular-smooth-muscle-cells
#18
Anna Huhtinen, Vesa Hongisto, Asta Laiho, Eliisa Löyttyniemi, Dirk Pijnenburg, Mika Scheinin
BACKGROUND: α2-adrenoceptors are important regulators of vascular tone and blood pressure. Regulation of cell proliferation is a less well investigated consequence of α2-adrenoceptor activation. We have previously shown that α2B-adrenoceptor activation stimulates proliferation of vascular smooth muscle cells (VSMCs). This may be important for blood vessel development and plasticity and for the pathology and therapeutics of cardiovascular disorders. The underlying cellular mechanisms have remained mostly unknown...
June 28, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/28655510/collagen-xiv-and-a-related-recombinant-fragment-protect-human-vascular-smooth-muscle-cells-from-calcium-phosphate-induced-osteochondrocytic-transdifferentiation
#19
Christian Freise, Veronika Bobb, Uwe Querfeld
Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I & IV (CI, CIV) along with collagen XIV (CXIV) and a CXIV-derived fragment (CXIV-F), both of which induce differentiation, were applied in an in-vitro model of calcium-/phosphate (Ca/P)-induced osteochondrocytic transdifferentiation of human and murine VSMC...
June 24, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28654057/murine-aortic-crush-injury-an-efficient-in-vivo-model-of-smooth-muscle-cell-proliferation-and-endothelial-function
#20
Dan Yu, George Makkar, Rajabrata Sarkar, Dudley K Strickland, Thomas S Monahan
Arterial reconstruction, whether angioplasty or bypass surgery, involves iatrogenic trauma causing endothelial disruption and vascular smooth muscle cell (VSMC) proliferation. Common murine models study small vessels such as the carotid and femoral arteries. Herein we describe an in vivo system in which both VSMC proliferation and endothelial barrier function can be simultaneously assessed in a large vessel. We studied the infrarenal aortic response to injury in C57BL/6 mice. The aorta was injured from the left renal vein to the aortic bifurcation by 30 transmural crushes of 5-seconds duration with a cotton-tipped applicator...
June 11, 2017: Journal of Visualized Experiments: JoVE
keyword
keyword
42800
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"