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https://www.readbyqxmd.com/read/28219841/experimental-and-computational-surface-hydrophobicity-analysis-of-a-non-enveloped-virus-and-proteins
#1
Caryn L Heldt, Amna Zahid, K Saagar Vijayaragavan, Xue Mi
The physical characteristics of viruses needs to be understood in order to manipulate the interaction of viruses with host cells, as well as to create specific molecular recognition techniques to detect, purify, and remove viruses. Viruses are generally believed to be positively charged at physiological pH, but there are few other defining characteristics. Here, we have experimentally and computationally demonstrated that a non-enveloped virus is more hydrophobic than a panel of model proteins. Reverse-phase and hydrophobic interaction chromatography and ANS fluorescence determined the experimental hydrophobic strength of each entity...
February 13, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28215695/inactivation-of-viruses-by-pasteurization-at-60%C3%A2-%C3%A2-c-for-10%C3%A2-h-with-and-without-40-glucose-as-stabilizer-during-a-new-manufacturing-process-of-%C3%AE-2-macroglobulin-from-cohn-fraction-iv
#2
Chaoji Huangfu, Yuyuan Ma, Junting Jia, Maomin Lv, Fengxuan Zhu, Xiaowei Ma, Xiong Zhao, Jingang Zhang
Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28210815/structural-proteins-of-helicoverpa-armigera-densovirus-2-enhance-transcription-of-viral-genes-through-transactivation
#3
Pengjun Xu, He Yuan, Xianming Yang, Robert I Graham, Kaiyu Liu, Kongming Wu
Herein, we report the identification of putative promoters for the non-structural proteins (NS) and capsid structural proteins (VP) of Helicoverpa armigera densovirus (HaDV2) as well as a potential mechanism for how these promoters might be regulated. For the first time, we report that VP is able to transactivate the VP promoter and, to a lesser degree, the NS promoter in densoviruses. In addition to this, another promoter-like sequence designated P2, when co-transfected with the VP gene, enhanced luciferase activity by approximately 35 times compared to a control...
February 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28196283/detection-of-human-parvovirus-b19-dna-in-22-of-1-815-cutaneous-biopsies-of-a-wide-variety-of-dermatologic-conditions-suggests-viral-persistence-after-primary-infection-and-casts-doubts-on-its-pathogenic-significance
#4
C Santonja, A Santos-Briz, G Palmedo, H Kutzner, L Requena
BACKGROUND: Human parvovirus B19 (B19V) has been associated with a number of dermatologic and systemic conditions, including myocarditis and autoimmune syndromes. OBJECTIVES: To determine the frequency of B19V deoxyribonucleic acid (DNA) detection in a large dermatopathology practice, and to characterize the histopathologic patterns involved. METHODS: We selected for polymerase chain reaction (PCR) detection of B19V a total of 1,815 skin biopsies pertaining to entities allegedly related to Parvovirus B19, as well as cases suspected clinically of representing paraviral exanthemas...
February 14, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28192048/a-serosurvey-of-diseases-of-free-ranging-gray-wolves-canis-lupus-in-minnesota
#5
Michelle Carstensen, John H Giudice, Erik C Hildebrand, J P Dubey, John Erb, Dan Stark, John Hart, Shannon Barber-Meyer, L David Mech, Steve K Windels, Andrew J Edwards
We tested serum samples from 387 free-ranging wolves ( Canis lupus ) from 2007 to 2013 for exposure to eight canid pathogens to establish baseline data on disease prevalence and spatial distribution in Minnesota's wolf population. We found high exposure to canine adenoviruses 1 and 2 (88% adults, 45% pups), canine parvovirus (82% adults, 24% pups), and Lyme disease (76% adults, 39% pups). Sixty-six percent of adults and 36% of pups exhibited exposure to the protozoan parasite Neospora caninum . Exposure to arboviruses was confirmed, including West Nile virus (37% adults, 18% pups) and eastern equine encephalitis (3% adults)...
February 13, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28192039/endemic-infection-of-stranded-southern-sea-otters-enhydra-lutris-nereis-with-novel-parvovirus-polyomavirus-and-adenovirus
#6
Juliana D Siqueira, Terry Ng, Melissa Miller, Linlin Li, Xutao Deng, Erin Dodd, Francesca Batac, Eric Delwart
Over the past century, the southern sea otter (SSO; Enhydra lutris nereis) population has been slowly recovering from near extinction due to overharvest. The SSO is a threatened species under federal law and a fully protected species under California law. Through a multiagency collaborative program, stranded animals are rehabilitated and released, while deceased animals are necropsied and tissues are cryopreserved to facilitate scientific study. Here, we processed archival tissues to enrich particle-associated viral nucleic acids, which we randomly amplified and deeply sequenced to identify viral genomes through sequence similarities...
February 13, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28184291/human-parvovirus-4-parv4-remains-elusive-despite-a-decade-of-study
#7
REVIEW
Philippa C Matthews, Colin Sharp, Peter Simmonds, Paul Klenerman
Human parvovirus 4 ('PARV4') is a small DNA tetraparvovirus, first reported in 2005. In some populations, PARV4 infection is uncommon, and evidence of exposure is found only in individuals with risk factors for parenteral infection who are infected with other blood-borne viruses. In other settings, seroprevalence studies suggest an endemic, age-associated transmission pattern, independent of any specific risk factors. The clinical impact of PARV4 infection remains uncertain, but reported disease associations include an influenza-like syndrome, encephalitis, acceleration of HIV disease, and foetal hydrops...
2017: F1000Research
https://www.readbyqxmd.com/read/28161113/-myocarditis
#8
G Hékimian, A Combes
Acute myocarditis must be considered in patients with recent onset of cardiac failure or arrhythmia. Fulminant myocarditis is a distinct entity characterized by sudden onset of severe congestive heart failure or cardiogenic shock, usually following a flu-like illness, parvovirus B19, human herpesvirus 6, coxsackie virus and adenovirus being the most frequently viruses responsible for the disease. In this setting, early recognition of patients rapidly progressing to refractory cardiac failure and their immediate transfer to a medical-surgical center experienced in mechanical circulatory support is warranted...
February 1, 2017: La Revue de Médecine Interne
https://www.readbyqxmd.com/read/28154460/antiviral-activity-of-a-novel-composition-of-peracetic-acid-disinfectant-on-parvoviruses
#9
Fadi Dagher, Jun Jiang, Peter Tijssen, Jean-François Laliberté
Porcine parvoviruses (PPV) are known to be particularly resistant to many disinfectants used to control other non-enveloped viruses. However, effective disinfectants used against PPV are harsh and corrosive to animal health facilities and the environment. We propose a noncorrosive "green" disinfectant that generates peracetic acid in-situ and is capable of inactivating PPV completely at a 1% concentration for a 10-minute contact time.
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28149707/virucidal-efficacy-of-a-sonicated-hydrogen-peroxide-system-trophon-%C3%A2-epr-following-european-and-german-test-methods
#10
Britta Becker, Birte Bischoff, Florian H H Brill, Eike Steinmann, Jochen Steinmann
Aim: The virucidal efficacy of an automated ultrasound probe disinfector (trophon(®) EPR) was evaluated in a three step procedure according to European and German test methods. This system uses sonicated hydrogen peroxide mist (35%) at elevated temperature (50°C) in a closed chamber with control of all parameters within a 7 minute cycle. Methods: In the first step of examination, the peroxide solution was tested in a quantitative suspension assay according to the Guideline of Deutsche Vereinigung zur Bekämpfung der Viruskrankheiten (DVV) e...
2017: GMS Hygiene and Infection Control
https://www.readbyqxmd.com/read/28142135/frequency-and-genotype-of-human-parvovirus-b19-among-iranian-hemodialysis-and-peritoneal-dialysis-patients
#11
Alireza Sharif, Arezoo Aghakhani, Ali Akbar Velayati, Mohammad Banifazl, Mohammad Reza Sharif, Effat Razeghi, Davood Kheirkhah, Monireh Kazemimanesh, Anahita Bavand, Amitis Ramezani
OBJECTIVES: The aim of this study was to evaluate the frequency and genotype of human parvovirus B19 and its relation with anemia among Iranian patients under dialysis. METHODS: Fifty hemodialysis (HD) and 33 peritoneal dialysis (PD) patients were enrolled. B19 IgG and IgM antibodies were assessed by ELISA, and the presence of B19 DNA was evaluated by nested PCR. PCR products were sequenced directly and phylogenetic analysis was performed. RESULTS: In the HD group, the prevalence of B19 antibodies was 54% for IgG and 4% for IgM...
2016: Intervirology
https://www.readbyqxmd.com/read/28135131/health-survey-of-free-ranging-raccons-procyon-lotor-in-central-park-new-york-new-york-usa-implications-for-human-and-domestic-animal-health
#12
Kimberly L Rainwater, Krysten Marchese, Sally Slavinski, Lee A Humberg, Edward J Dubovi, Jodie A Jarvis, Denise McAloose, Paul P Calle
We conducted health assessments on 113 free-ranging raccoons ( Procyon lotor ) in Central Park, New York City, in February 2010, September 2010, and November 2011 in conjunction with a trap-vaccinate-release program to control a raccoon rabies epizootic. Five individuals were sampled at two time points for 118 raccoon examinations in total. We tested 13 of 13 and 8 of 13 euthanized raccoons for rabies and canine distemper virus, respectively, by antigen testing on brain tissue; all were negative for both viruses...
January 30, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28128088/gestational-pityriasis-rosea-suggestions-for-approaching-affected-pregnant-women
#13
Alexandra Monastirli, Efstathia Pasmatzi, George Badavanis, Dionysios Tsambaos
Dear Editor, Pityriasis rosea is a common, acute, and self-limiting dermatosis, which is associated with the endogenous systemic reactivation of human herpesvirus (HHV)-6 and/or HHV-7 (1). It predominantly affects individuals of both sexes in their second or third decade of life and is clinically characterized by the occurrence of an initial erythematosquamous plaque followed by the appearance of disseminated similar but smaller lesions one or two weeks later. Several patients develop systemic symptoms such as nausea, anorexia, malaise, headache, fever, arthralgia, and lymphadenopathy that may precede or accompany the eruption; the latter follows the cleavage lines of the trunk creating the configuration of a Christmas tree and spontaneously resolves within 4 to 8 weeks...
December 2016: Acta Dermatovenerologica Croatica: ADC
https://www.readbyqxmd.com/read/28125695/novel-mutant-aav2-rep-proteins-support-aav2-replication-without-blocking-hsv-1-helpervirus-replication
#14
Michael Seyffert, Daniel L Glauser, Elisabeth M Schraner, Anna-Paula de Oliveira, Jorge Mansilla-Soto, Bernd Vogt, Hildegard Büning, R Michael Linden, Mathias Ackermann, Cornel Fraefel
As their names imply, parvoviruses of the genus Dependovirus rely for their efficient replication on the concurrent presence of a helpervirus, such as herpesvirus, adenovirus, or papilloma virus. Adeno-associated virus 2 (AAV2) is such an example, which in turn can efficiently inhibit the replication of each helpervirus by distinct mechanisms. In a previous study we have shown that expression of the AAV2 rep gene is not compatible with efficient replication of herpes simplex virus 1 (HSV-1). In particular, the combined DNA-binding and ATPase/helicase activities of the Rep68/78 proteins have been shown to exert opposite effects on the replication of AAV2 and HSV-1...
2017: PloS One
https://www.readbyqxmd.com/read/28125171/prevalence-of-increased-canine-pancreas-specific-lipase-concentrations-in-young-dogs-with-parvovirus-enteritis
#15
Irida V Kalli, Katerina K Adamama-Moraitou, Michael N Patsika, Dimitra Pardali, Jörg M Steiner, Jan S Suchodolski, George Menexes, Georgia D Brellou, Timoleon S Rallis
BACKGROUND: Pancreatic abnormalities during canine parvovirus (CPV) enteritis have not been studied prospectively. OBJECTIVES: The objective of this study was to evaluate the diagnostic significance of canine serum pancreas-specific lipase (Spec cPL) concentration in dogs with CPV enteritis for the presence of acute pancreatitis (AP). Puppies with naturally occurring CPV enteritis were recruited and prospectively allocated into 2 groups according to normal or increased serum Spec cPL concentration...
January 26, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28124140/molecular-characterization-of-canine-parvovirus-in-vientiane-laos
#16
Soulasack Vannamahaxay, Souliya Vongkhamchanh, Montira Intanon, Sahatchai Tangtrongsup, Saruda Tiwananthagorn, Kidsadagon Pringproa, Phongsakorn Chuammitri
The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of dogs were CPV positive by PCR. The partial VP2 gene was sequenced in 94 of the positive samples; 91 samples belonged to CPV-2c (426Glu) subtype, while 3 samples belonged to the CPV-2a (426Asn) subtype...
January 25, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28122984/parvovirus-expresses-a-small-noncoding-rna-that-plays-an-essential-role-in-virus-replication
#17
Zekun Wang, Weiran Shen, Fang Cheng, Xuefeng Deng, John F Engelhardt, Ziying Yan, Jianming Qiu
: Human bocavirus 1 (HBoV1) belongs to species Primate bocaparvovirus of the genus Bocaparvovirus of the Parvoviridae family. HBoV1 causes acute respiratory tract infections in young children and has a selective tropism for the apical surface of well-differentiated human airway epithelia (HAE). In this study, we identify an additional HBoV1 gene, bocavirus-transcribed small non-coding RNA (BocaSR) within the 3' non-coding region (nt 5199-5338) of the viral genome of positive sense. BocaSR is transcribed by RNA polymerase III (Pol III) from an intragenic promoter at similar levels to that of the capsid protein-coding mRNA and is essential for replication of the viral DNA in both transfected HEK293 and infected HAE cells...
January 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28116652/does-parvovirus-infection-have-a-role-in-systemic-lupus-erythematosus
#18
Tami Hod, Giselle Zandman-Goddard, Pnina Langevitz, Hagit Rudnic, Zehava Grossman, Pnina Rotman-Pikielny, Yair Levy
We sought to evaluate a possible link between parvovirus B19 infection and the clinical and laboratory expression of systemic lupus erythematosus (SLE). SLE patients were examined to evaluate their clinical status and disease activity. A complete Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score was obtained for each patient. In addition, we determined the level of systemic involvement throughout the course of the disease. Blood levels of IgM and IgG antibodies to parvovirus B19, levels of anti-dsDNA, C3, and C4 were measured...
January 23, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28109291/systemic-nocardiosis-in-a-dog-caused-by-nocardia-cyriacigeorgica
#19
Yesari Eroksuz, Nafia Canan Gursoy, Tolga Karapinar, Burak Karabulut, Canan Akdeniz Incili, Zeynep Yerlikaya, Zulal Asci Toraman, Mehmet Ozkan Timurkan, Hatice Eroksuz
BACKGROUND: Systemic nocardiosis due to Nocardia cyriacigeorgica has not been reported in dogs. CASE PRESENTATION: Light and electron microscopy, microbiological culture and molecular identification (PCR) were used to diagnose systemic nocardiosis caused by Nocardia cyriacigeorgica in a 3-month-old husky dog. The postmortem changes included multifocal to coalescing, sharply circumscribed pyogranulomatous inflammation and abscess formation in lungs, liver, myocardium, spleen, kidneys, brain, and hilar lymph nodes...
January 21, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28100412/virome-of-us-bovine-calf-serum
#20
Mohammadreza Sadeghi, Beatrix Kapusinszky, Danielle M Yugo, Tung Gia Phan, Xutao Deng, Isis Kanevsky, Tanja Opriessnig, Amelia R Woolums, David J Hurley, Xiang-Jin Meng, Eric Delwart
Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses...
January 15, 2017: Biologicals: Journal of the International Association of Biological Standardization
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