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https://www.readbyqxmd.com/read/28716388/identification-of-co-infection-by-rotavirus-and-parvovirus-in-dogs-with-gastroenteritis-in-mexico
#1
Ariadna Flores Ortega, José Simón Martínez-Castañeda, Linda G Bautista-Gómez, Raúl Fajardo Muñoz, Israel Quijano Hernández
This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe.
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28716118/evolutionary-and-genetic-analysis-of-the-vp2-gene-of-canine-parvovirus
#2
Gairu Li, Senlin Ji, Xiaofeng Zhai, Yuxiang Zhang, Jie Liu, Mengyan Zhu, Jiyong Zhou, Shuo Su
BACKGROUND: Canine parvovirus (CPV) type 2 emerged in 1978 in the USA and quickly spread among dog populations all over the world with high morbidity. Although CPV is a DNA virus, its genomic substitution rate is similar to some RNA viruses. Therefore, it is important to trace the evolution of CPV to monitor the appearance of mutations that might affect vaccine effectiveness. RESULTS: Our analysis shows that the VP2 genes of CPV isolated from 1979 to 2016 are divided into six groups: GI, GII, GIII, GIV, GV, and GVI...
July 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28715672/genomic-and-pathogenic-analysis-of-a-muscovy-duck-parvovirus-strain-causing-short-beak-and-dwarfism-syndrome-without-tongue-protrusion
#3
Qiuling Fu, Yu Huang, Chunhe Wan, Guanghua Fu, Baomin Qi, Longfei Cheng, Shaohua Shi, Hongmei Chen, Rongchang Liu, Zhenhai Chen
In 2008, clinical cases of short beak and dwarfism syndrome (SBDS) caused by Muscovy duck parvovirus (MDPV) infection were found in mule duck and Taiwan white duck farms in Fujian, China. A MDPV LH strain causing duck SBDS without tongue protrusion was isolated in this study. Phylogenetic analysis show that the MDPV LH strain was clustered together with other MDPV strains, but divergent from GPV isolates. Two major fragment deletions were found in the inverted terminal repeats (ITR) of MDPV LH similar to the ones in the ITR of MDPV GX5, YY and SAAS-SHNH strains...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28715293/surveillance-for-antibodies-against-six-canine-viruses-in-wild-raccoons-procyon-lotor-in-japan
#4
Emiko Aoki, Takehisa Soma, Mayumi Yokoyama, Makoto Matsubayashi, Kazumi Sasai
Raccoons (Procyon lotor) are found worldwide. They are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. We examined sera from 100 wild raccoons in Japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. We also aimed to understand the distribution of potentially infected wildlife. We found that 7% of samples were seropositive for canine distemper virus (CDV), 10% for canine parvovirus type (CPV), 2% for canine adenovirus type 1, 6% for canine adenovirus type 2, and 7% for canine coronavirus...
July 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28707272/first-molecular-characterization-of-canine-parvovirus-strains-in-sardinia-italy
#5
S Dei Giudici, T Cubeddu, A Giagu, G Sanna, S Rocca, A Oggiano
Canine parvovirus type 2 (CPV-2) is responsible of acute hemorrhagic gastroenteritis in young dogs. CPV-2 emerged in 1978 in the USA, but new antigenic types, CPV-2a, 2b and 2c, have completely replaced the original type. In this study, we analyzed 81 animals collected in Sardinia, Italy. The VP2 sequence analysis of 27 positive samples showed that all antigenic CPV-2 types are circulating. CPV-2b seems to be the most widespread variant, followed by CPV-2a. Furthermore, 12 CPV-2b strains displayed further amino acid substitutions and formed a separate cluster in a phylogenetic tree, indicating regional genetic variation...
July 14, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28700372/immunohistochemical-demonstration-of-parvovirus-b19-viral-protein-2-in-periflexural-exanthema-in-an-adult-supporting-antibody-dependent-enhancement-as-means-of-endothelial-uptake-of-the-virus
#6
Carlos Santonja, Úrsula Pielasinski, Jorge Polo, Heinz Kutzner, Luis Requena
Human parvovirus B19 (B19V) causes a number of skin exanthemas and has been related to both cutaneous and systemic diseases. Tropism of the virus for the rapidly proliferating erythroid progenitor cells in the bone marrow and fetal liver explains the pathogenesis of anemia and fetal hydrops. The cutaneous lesions of erythema infectiosum and other B19V-related exanthemas have been attributed to the deposition of immune complexes in the skin. We report on the immunohistochemical detection of B19V protein in the cytoplasm of dermal endothelial cells in a case of periflexural exanthema in a 28-year-old woman...
July 6, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28696303/intestinal-virome-changes-precede-autoimmunity-in-type-i-diabetes-susceptible-children
#7
Guoyan Zhao, Tommi Vatanen, Lindsay Droit, Arnold Park, Aleksandar D Kostic, Tiffany W Poon, Hera Vlamakis, Heli Siljander, Taina Härkönen, Anu-Maaria Hämäläinen, Aleksandr Peet, Vallo Tillmann, Jorma Ilonen, David Wang, Mikael Knip, Ramnik J Xavier, Herbert W Virgin
Viruses have long been considered potential triggers of autoimmune diseases. Here we defined the intestinal virome from birth to the development of autoimmunity in children at risk for type 1 diabetes (T1D). A total of 220 virus-enriched preparations from serially collected fecal samples from 11 children (cases) who developed serum autoantibodies associated with T1D (of whom five developed clinical T1D) were compared with samples from controls. Intestinal viromes of case subjects were less diverse than those of controls...
July 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28690087/an-exo-probe-based-recombinase-polymerase-amplification-assay-for-the-rapid-detection-of-porcine-parvovirus
#8
Jian-Chang Wang, Li-Bing Liu, Qing-An Han, Jin-Feng Wang, Wan-Zhe Yuan
Recombinase polymerase amplification (RPA), an isothermal amplification technology, has been developed as an alternative to PCR in pathogen detection. A real-time RPA assay (rt-RPA) was developed to detect the porcine parvovirus (PPV) using primers and exo probe specific for the VP2 gene. The amplification was performed at 39°C for 20min. There was no cross-reaction with other pathogens tested. Using the recombinant plasmid pPPV-VP2 as template, the analytical sensitivity was 103 copies. The assay performance was evaluated by testing 115 field samples by rt-RPA and a real-time PCR assay...
July 6, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28689674/determination-of-immune-status-in-dogs-against-cpv-2-by-recombinant-protein-based-latex-agglutination-test
#9
Jobin Thomas, Mithilesh Singh, T K Goswami, Philma Glora, Soumendu Chakravarti, Vishal Chander, Vikramaditya Upmanyu, Suman Verma, Chhavi Sharma, K Mahendran
Canine parvoviral enteritis is a highly contagious viral illness caused by canine parvovirus-2 (CPV-2) which affects puppies of mainly 6-20 weeks of age. Vaccination is pivotal in preventing and controlling CPV-2 infection. Determination of antibody status is a critical determinant for successful vaccination. The hemagglutination inhibition (HI) test is 'gold standard' test for quantification of antibodies specific to CPV-2, although the execution of this test is not feasible under field conditions. The present study was undertaken to develop a point of care testing to determine immune status prior to CPV-2 vaccination or to detect seroconversion in immunized dogs by latex agglutination test (LAT) using recombinant antigen...
July 6, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28679762/aav-serotypes-have-distinctive-interactions-with-domains-of-the-cellular-receptor-aavr
#10
Sirika Pillay, Wei Zou, Fang Cheng, Andreas S Puschnik, Nancy L Meyer, Safder S Ganaie, Xuefeng Deng, Jonathan E Wosen, Omar Davulcu, Ziying Yan, John F Engelhardt, Kevin E Brown, Michael S Chapman, Jianming Qiu, Jan E Carette
Adeno-associated virus (AAV) entry is determined by its interactions with specific surface glycans and proteinaceous receptor(s). Adeno-associated virus receptor (AAVR; also named KIAA0319L) is an essential cellular receptor required for the transduction of vectors derived from multiple AAV serotypes including the evolutionary distant serotypes, AAV2 and AAV5. Here, we further biochemically characterize the AAV-AAVR interaction and define the domains within the ectodomain of AAVR that facilitate this interaction...
July 5, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28669382/new-wide-dynamic-range-assays-for-quantification-of-anti-leishmania-igg2-and-iga-antibodies-in-canine-serum
#11
Ana Cantos-Barreda, Damián Escribano, Luis J Bernal, Luis Pardo-Marín, José J Cerón, Silvia Martínez-Subiela
The aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-Leishmania IgG2 and IgA antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-Leishmania antibodies in Leishmania-seronegative and seropositive dogs, determined by a commercial ELISA assay, and between different clinical stages according to LeishVet guidelines. The analytical validation showed that the assays had a good precision with intra- and inter-assay coefficients of variation lower than 10%...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28665873/induction-failure-in-acute-leukemia-or-parvovirus-b19-infection
#12
Özlem Tüfekçi, Şebnem Yilmaz Bengoa, Melek Erdem, Hale Ören
Parvovirus B19 infection may be seen in acute leukemia patients and clinical findings and cytopenia caused by the viral infection may complicate the evaluation of the remission status. Herein we present a standard risk pediatric acute lymphobiastic leukemia patient who developed myalgia, bone pain, bone marrow aplasia and sinusoidal obstruction syndrome at the end of the induction treatment and was diagnosed as having parvovirus B19 infection.
June 29, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28662150/a-viral-metagenomic-approach-on-a-non-metagenomic-experiment-mining-next-generation-sequencing-datasets-from-pig-dna-identified-several-porcine-parvoviruses-for-a-retrospective-evaluation-of-viral-infections
#13
Samuele Bovo, Gianluca Mazzoni, Anisa Ribani, Valerio Joe Utzeri, Francesca Bertolini, Giuseppina Schiavo, Luca Fontanesi
Shot-gun next generation sequencing (NGS) on whole DNA extracted from specimens collected from mammals often produces reads that are not mapped (i.e. unmapped reads) on the host reference genome and that are usually discarded as by-products of the experiments. In this study, we mined Ion Torrent reads obtained by sequencing DNA isolated from archived blood samples collected from 100 performance tested Italian Large White pigs. Two reduced representation libraries were prepared from two DNA pools constructed each from 50 equimolar DNA samples...
2017: PloS One
https://www.readbyqxmd.com/read/28659484/case-control-comparison-of-enteric-viromes-in-captive-rhesus-macaques-with-acute-or-idiopathic-chronic-diarrhea
#14
Beatrix Kapusinszky, Amir Ardeshir, Usha Mulvaney, Xutao Deng, Eric Delwart
Diarrhea is the major cause of non-research associated morbidity and mortality affecting the supply of rhesus macaques and, potentially, their responses to experimental treatments. Idiopathic chronic diarrhea (ICD) in rhesus macaques also resembles ulcerative colitis, a form of human inflammatory bowel disease. To test for viral etiologies we characterized and compared the fecal virome from 32 healthy, 31 with acute diarrhea, and 29 with ICD animals. The overall fraction of eukaryotic viral reads was 0.063% for the healthy group, 0...
June 28, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28659483/human-bocavirus-1-is-a-novel-helper-for-adeno-associated-virus-replication
#15
Zekun Wang, Xuefeng Deng, Wei Zou, John F Engelhardt, Ziying Yan, Jianming Qiu
Human bocavirus 1 (HBoV1) is an autonomous parvovirus that infects well-differentiated primary human airway epithelia (HAE) in vitro. In human embryonic kidney (HEK) 293 cells, transfection of a duplex HBoV1 genome initiates viral DNA replication and produces progeny virions that are infectious in HAE. HBoV1 takes advantage of signaling pathways in the DNA damage response for efficient genome amplification in both well-differentiated (non-dividing) HAE and dividing HEK293 cells. On the other hand, adeno-associated virus 2 (AAV2) is a helper-dependent dependoparvovirus, and productive AAV2 replication requires co-infection with a helper virus (e...
June 28, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28657857/genetic-characterization-of-canine-parvovirus-in-sympatric-free-ranging-wild-carnivores-in-portugal
#16
Carla Miranda, Nuno Santos, Colin Parrish, Gertrude Thompson
Since its emergence in the 1970s, canine parvovirus (CPV) has been reported in domestic and nondomestic carnivores worldwide with severe implications on their health and survival. Here, we aim to better understand CPV circulation in multihost-pathogens systems by characterizing CPV DNA or viruses in 227 free-ranging wild carnivores of 12 species from Portugal. Collected samples during 1995-2011 were analyzed by PCR and sequence analysis. The canine parvovirus DNA was detected in 4 (2%) animals of two species, namely in wolves (Canis lupus; 3/63, 5%, 95% confidence interval=1...
June 28, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28657532/-human-bocavirus-1-role-in-the-acute-respiratory-infection-and-epidemiology-in-cordoba-argentina
#17
REVIEW
María Pilar Adamo
Human bocavirus 1 (HBoV1) is an agent of acute respiratory infection frequent in children. It can cause pneumonia in infants, in the absence of epidemiological risk factors and comorbidities. Well-controlled studies of clinical cases and case series are still useful for the characterization of the clinicoepideiological features of the infection, while research dives on the molecular biology of the virus and the virus-cell relationship allowing to unveil tha natural history of the infection. This article reviews the state of the art and future perspectives on this new human parvovirus and its etiological role in the respiratory pathology...
2017: Revista de la Facultad de Ciencias Médicas
https://www.readbyqxmd.com/read/28639277/serum-derived-transfer-factor-stimulates-the-innate-immune-system-to-improve-survival-traits-in-high-risk-pathogen-scenarios
#18
Bridget V Willeford, Trudy Shapiro-Dunlap, Kenneth O Willeford
Preclinical Research Transfer Factors (TFs) are low molecular weight (<5,000 daltons) biological response mediators. In the present study, a serum derived TF improved the ability of the recipient animal to survive high-risk infectious challenges (salmonellosis and canine parvoviral enteritis (CPV)) by altering the host's cytokine response profile. Mice mortally challenged with 5,000 colony-forming units of Salmonella experienced a group mortality of 73% while mice treated with a single 5 mg dose of the TF demonstrated a significant decrease in morbidity (7%, p ≤ 0...
June 22, 2017: Drug Development Research
https://www.readbyqxmd.com/read/28635524/serology-and-protein-electrophoresis-for-evidence-of-exposure-to-12-mink-pathogens-in-free-ranging-american-mink-neovison-vison-in-argentina
#19
Pablo Eduardo Martino, Luis Ernesto Samartino, Néstor Oscar Stanchi, Nilda Esther Radman, Eduardo Joaquín Parrado
BACKGROUND: Basic pathologic characteristics for farmed minks were previously reported worldwide. However, its status in the wild has not been studied in detail. OBJECTIVE: Serology and electrophoresis were carried out for evidence of exposure to 12 mink pathogens on two different locations. ANIMALS AND METHODS: Serology was done in 87 wild minks by reference techniques against Toxoplasma gondii, Encephalitozoon cuniculi, Neospora caninum, Brucella abortus, Mycobacterium bovis, Leptospira interrogans, canine distemper virus (CDV), canine adenovirus (CAV), canine parvovirus (CPV), rabies virus (RV), Influenza A virus (FLUAV) and Aleutian disease virus (ADV)...
December 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28633740/parvovirus-b19-infection-in-an-adult-presenting-with-connective-tissue-disease-like-symptoms-a-report-of-the-clinical-and-histological-findings
#20
J E Liles, S C Shalin, B A White, L B Trigg, J R Kaley
Parvovirus B19 infections in adults are usually associated with nonspecific and mild symptoms. However, cases presenting with a lupus-like syndrome have been described, leading to the hypothesis that parvovirus infection can induce connective tissue disease. Various histopathologic features of cutaneous manifestations of parvovirus have been reported, including features which overlap with those of connective tissue disease. Herein, we discuss an unusual case of Parvovirus  B19 infection in a middle-aged woman...
June 15, 2017: Dermatology Online Journal
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