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David Juhl, Holger Hennig
Parvovirus B19 (B19V) has been discovered in 1975. The association with a disease was unclear in the first time after the discovery of B19V, but meanwhile, the usually droplet transmitted B19V is known as the infectious agent of the "fifth disease," a rather harmless children's illness. But B19V infects erythrocyte progenitor cells and thus, acute B19V infection in patients with a high erythrocyte turnover may lead to a life-threatening aplastic crisis, and acutely infected pregnant women can transmit B19V to their unborn child, resulting in a hydrops fetalis and fetal death...
2018: Frontiers in Medicine
Safder S Ganaie, Aaron Yun Chen, Chun Huang, Peng Xu, Steve Kleiboeker, Aifang Du, Jianming Qiu
Human parvovirus B19 (B19V) expresses a single precursor mRNA (pre-mRNA), which undergoes alternative splicing and alternative polyadenylation to generate 12 viral mRNA transcripts that encode two structural proteins (VP1 and VP2) and three nonstructural proteins (NS1, 7.5-kDa, and 11-kDa). Splicing at the second 5' donor site (D2) of the B19V pre-mRNA is essential for the expression of VP2 and 11-kDa. We have previously identified that a cis -acting intronic splicing enhancer 2 (ISE2) that lies immediately after the D2 site facilitates recognition of the D2 donor for its efficient splicing...
February 7, 2018: Journal of Virology
Alisha Floyd, Ashwin Lal, Kimberly Molina, Michael Puchalski, Dylan Miller, Lindsay May
Myocarditis is an important but incompletely understood cause of cardiac dysfunction. Children with fulminant myocarditis often require inotropic or mechanical circulatory support, and researchers in some studies suggest that up to 42% of children who die suddenly have evidence of myocarditis. Recurrent myocarditis is extremely rare, and the vast majority of reported cases involve adult patients. Pediatric providers who suspect a recurrence of myocarditis have limited evidence to guide patient management because the literature in this domain is sparse...
February 8, 2018: Pediatrics
Shan Ma, Guang Li Pang, Yu Juan Shao, Tomoko Hongo-Hirasaki, Meng Xian Shang, Marcus Inouye, Chang Yong Jian, Meng Zhao Zhu, Hu Hu Yang, Jian Feng Gao, Zhi Ying Xi, Dian Wei Song
There is a continuous need to improve the viral safety of plasma products, and we here report the development and optimization of a manufacturing-scale virus removal nanofiltration step for intravenous immunoglobulin (IVIG) using the recently introduced Planova™ BioEX filter. IVIG throughput was examined for various operating parameters: transmembrane pressure, temperature, protein concentration, and prefiltration methods. The developed procedure was based on filtering undiluted process solution (50.0 g/l IVIG) under constant transmembrane pressure filtration at 294 kPa and 25 °C following prefiltration with a 0...
February 9, 2018: Biologicals: Journal of the International Association of Biological Standardization
Aida Zečkanović, Matej Perovnik, Janez Jazbec, Miroslav Petrovec, Marko Pokorn, Marko Kavčič
We report a case of a 12-year-old male with glucose-6-phosphate dehydrogenase deficiency presenting with clinical signs of sepsis and pancytopenia. Investigations revealed parvovirus B19 (PVB19)-associated hemophagocytic lymphohistiocytosis (HLH). The patient recovered fully and quickly with symptomatic treatment. Current evidence suggests that PVB19-associated HLH has a favorable prognosis. Mild undiagnosed cases of HLH may be the cause of pancytopenia in PVB19 infections.
February 9, 2018: Journal of Pediatric Hematology/oncology
Adriana Christie Lacerda Simões, Tinara Leila de Souza Aarão, Jorge Rodrigues de Sousa, Diogo Lima Prudente, Yaisa Gomes de Castro, Rodrigo Bona Maneschy, Hellen Thais Fuzii, Juarez Antonio Simões Quaresma
Parvovirus B19 (PVB19) is a virus found in the skin that causes asymptomatic infections and can exist in the host for long periods to time. The virus induces a local inflammatory response and is associated with the development of arthritis and other autoimmunes diseases. Parvovirus B19 DNA was investigated by PCR in the skin of 20 patients with psoriasis and 20 patients with eczema. Additionally, immunohistochemistry was used to characterize the expression of cytokines in these lesions. The sociodemographic variables were similar in the two groups studied...
February 8, 2018: Microbial Pathogenesis
Hao Wang, Per Sikora, Carolin Rutgersson, Magnus Lindh, Tomas Brodin, Berndt Björlenius, D G Joakim Larsson, Heléne Norder
Sewage contains a mixed ecosystem of diverse sets of microorganisms, including human pathogenic viruses. Little is known about how conventional as well as advanced treatments of sewage, such as ozonation, reduce the environmental spread of viruses. Analyses for viruses were therefore conducted for three weeks in influent, after conventional treatment, after additional ozonation, and after passing an open dam system at a full-scale treatment plant in Knivsta, Sweden. Viruses were concentrated by adsorption to a positively charged filter, from which they were eluted and pelleted by ultracentrifugation, with a recovery of about 10%...
February 1, 2018: International Journal of Hygiene and Environmental Health
Andrea Balboni, Francesca Bassi, Stefano De Arcangeli, Rosanna Zobba, Carla Dedola, Alberto Alberti, Mara Battilani
BACKGROUND: Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants 2a, 2b and 2c. Detection of FPV and CPV variants in apparently healthy cats and their persistence in white blood cells (WBC) and other tissues when neutralising antibodies are simultaneously present, suggest that parvovirus may persist long-term in the tissues of cats post-infection without causing clinical signs. The aim of this study was to screen a population of 54 cats from Sardinia (Italy) for the presence of both FPV and CPV DNA within buffy coat samples using polymerase chain reaction (PCR)...
February 5, 2018: BMC Veterinary Research
Rongchang Liu, Cuiteng Chen, Yu Huang, Longfei Cheng, Ronghui Lu, Guanghua Fu, Shaohua Shi, Hongmei Chen, Chunhe Wan, Qiuling Fu, Jiansheng Lin
In total, 985 livers were collected from 275 backyard waterfowl farms distributed in seven provinces of southern China. The virus that was most commonly isolated was avian influenza virus, with a 12.1% positivity rate. Of the other positive samples, 10.6% tested positive for avian Tembusu virus, 6.8% for duck hepatitis A virus, 3.8% for duck plague virus, 3.4% for Muscovy duck parvovirus, 3.1% for goose parvovirus, 1.0% for mycoplasma, and 0.9% for respiratory enteric orphan virus. The bacterium that was most commonly isolated was Escherichia coli, with a 47...
February 2, 2018: Journal of Veterinary Medical Science
Yinjie Niu, Lili Zhao, Baihan Liu, Jingli Liu, Fan Yang, Haichang Yin, Hong Huo, Hongyan Chen
BACKGROUND: Goose parvovirus (GPV) causes acute enteritis, hepatitis, myocarditis and high morbidity and mortality in geese and ducks. GPV H strain was isolated from a Heilongjiang goose farm where the geese were showing signs of hemorrhage in the brain, liver, and intestinal tract. In this study, we explored the genetic diversity among waterfowl parvovirus isolates and the pathological characteristics of GPV H in Shaoxing ducklings. METHODS: The complete capsid protein (VP) and non-structural (NS) sequences of the isolated H strain were sequenced, and phylogenetic trees of VP and NS were constructed in MEGA version 5...
February 1, 2018: Virology Journal
Flávia V Vieira, Daniel J Hoffmann, Carolina U F Fabri, Katia D S Bresciani, Roberto Gameiro, Eduardo F Flores, Tereza C Cardoso
Despite of the role of domestic dogs as reservoirs for threatening viral diseases for wild carnivores, few studies have focused to identify circulation of viruses among dogs living in human/wildlife interfaces. To identify canine parvovirus (CPV) types circulating in dogs living in an Atlantic forest biome, faecal samples (n = 100) were collected at the same period (one week) corresponding to each of four areas, during 2014 to 2016 and corresponded to 100 different individuals. CPV was isolated in cell culture from 67 out 100 (67%) samples from healthy dogs...
December 2017: Heliyon
Matthew Marr, Anthony D'Abramo, Nikea Pittman, Mavis Agbandje-McKenna, Susan F Cotmore, Peter Tattersall
Combining virus-enhanced immunogenicity with direct delivery of immunomodulatory molecules would represent a novel treatment modality for melanoma, and would require development of new viral vectors capable of targeting melanoma cells preferentially. Here we explore the use of rodent protoparvoviruses targeting cells of the murine melanoma model B16F10. An uncloned stock of mouse parvovirus 1 (MPV1) showed some efficacy, which was substantially enhanced following serial passage in the target cell. Molecular cloning of the genes of both starter and selected virus pools revealed considerable sequence diversity...
January 30, 2018: Viruses
Anna Malmsten, Ulf Magnusson, Francisco Ruiz-Fons, David González-Barrio, Anne-Marie Dalin
The wild boar ( Sus scrofa) population has increased markedly during the last three decades in Sweden and in other parts of Europe. This population growth may lead to increased contact between the wild boar and the domestic pig ( Sus scrofa scrofa), increasing the risk of transmission of pathogens. The objective of our study was to estimate the seroprevalence of selective pathogens, known to be shared between wild boars and domestic pigs in Europe, in three wild boar populations in Sweden. In total, 286 hunter-harvested female wild boars were included in this study...
January 29, 2018: Journal of Wildlife Diseases
Dafei Liu, Fei Liu, Dongchun Guo, Xiaoliang Hu, Zhijie Li, Zhigang Li, Jianzhang Ma, Chunguo Liu
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.
January 23, 2018: Journal of Veterinary Medical Science
Liang Zhang, Zhenyu Wang, Jie Zhang, Xiaomao Luo, Qian Du, Lingling Chang, Xiaomin Zhao, Yong Huang, Dewen Tong
Porcine parvovirus (PPV) is a major virus leading to fetal death in swine. However, the effects of PPV infection on sows are poorly understood. The aim of this study was to investigate the effects of PPV on porcine steroidogenic luteal cells (SLCs) survival and functions and underlying mechanisms. In vivo experiment results showed artificial infection of PPV significantly reduced the concentration of serum progesterone and induced histopathological lesions and SLCs apoptosis in porcine corpora luteum. In vitro cultured primary porcine SLCs, PPV could infect and replicate in SLCs and induced SLCs apoptosis through mitochondria, but not the death receptor, mediated apoptosis pathway...
January 18, 2018: Biology of Reproduction
C Santonja, L Requena, J Polo Sabau, U Pielasinski Rodríguez
No abstract text is available yet for this article.
January 2018: British Journal of Dermatology
Kumaran Vadivel, Mageshbabu Ramamurthy, Sathish Sankar, Amita Jain, Padma Srikanth, Asit Ranjan Ghosh, Balaji Nandagopal, Aravindan Nair, Gopalan Sridharan
BACKGROUND & OBJECTIVES: Parvovirus B19 infections occur worldwide; the infection is acquired early in childhood but could occur later. B19 is reported to cause infection in childhood febrile illnesses, and arthropathies in adults and children and in end-stage renal disease (ESRD) seen in adults. This study was designed to develop an in-house IgM indirect ELISA for serological screening among patients and controls, and to compare ELISA results with those of nested polymerase chain reaction (nPCR) assay...
September 2017: Indian Journal of Medical Research
Nasir Idris Abdullahi, Medugu Jessy Thomas, Amos Dangana
No abstract text is available yet for this article.
January 18, 2018: Journal of Biomedical Research
Thomas J Divers, Bud C Tennant, Arvind Kumar, Sean McDonough, John Cullen, Nishit Bhuva, Komal Jain, Lokendra Singh Chauhan, Troels Kasper Høyer Scheel, W Ian Lipkin, Melissa Laverack, Sheetal Trivedi, Satyapramod Srinivasa, Laurie Beard, Charles M Rice, Peter D Burbelo, Randall W Renshaw, Edward Dubovi, Amit Kapoor
Equine serum hepatitis (i.e., Theiler's disease) is a serious and often life-threatening disease of unknown etiology that affects horses. A horse in Nebraska, USA, with serum hepatitis died 65 days after treatment with equine-origin tetanus antitoxin. We identified an unknown parvovirus in serum and liver of the dead horse and in the administered antitoxin. The equine parvovirus-hepatitis (EqPV-H) shares <50% protein identity with its phylogenetic relatives of the genus Copiparvovirus. Next, we experimentally infected 2 horses using a tetanus antitoxin contaminated with EqPV-H...
February 2018: Emerging Infectious Diseases
Elina Mäntylä, Jenu V Chacko, Vesa Aho, Colin R Parrish, Victor Shahin, Michael Kann, Michelle A Digman, Enrico Gratton, Maija Vihinen-Ranta
Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm...
January 18, 2018: Scientific Reports
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