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Tatjana Taubitz, Laura-Pia Steinbrenner, Alexander V Tschulakow, Antje Biesemeier, Sylvie Julien-Schraermeyer, Ulrich Schraermeyer
PURPOSE: Anti-vascular endothelial growth factor (VEGF) drugs are used to treat neovascular eye diseases. Some of these drugs contain Fc fragments (Fc), but it is unknown how their mode of action is influenced by Fc. Therefore, this study investigated the effects of Fc on rat eyes after intravitreal injection. METHODS: Eighteen Long-Evans rats were intravitreally injected with sterile, biotin-labeled rat Fc (9.1 μg in 5 μl PBS). For control, 5 μl PBS was injected in another nine rats...
October 17, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
Yoshiaki Takahashi, Toshiharu Matsuura, Yusuke Yanagi, Koichiro Yoshimaru, Tomoaki Taguchi
BACKGROUND/PURPOSE: There is currently no unified view regarding whether liver transplantation or splenectomy should be performed for hypersplenism before liver transplantation in biliary atresia (BA) patients. We herein describe the efficacy of splenectomy before liver transplantation. METHODS: Splenectomy was performed in ten patients with hypersplenism associated with BA. We retrospectively reviewed their perioperative and postoperative courses, the number of leukocytes and thrombocytes, and the MELD score...
September 26, 2016: Journal of Pediatric Surgery
Linda Tran, Khosrow Mottaghy, Sabine Arlt-Körfer, Christian Waluga, Mehdi Behbahani
Undesirable thrombotic reactions count among the most frequent and serious complications for patients who rely on the use of medical devices. To improve the design of medical devices, it is essential to develop a more precise understanding of platelet reactions. Clinical studies and experiments have shown a strong dependence of platelet deposition behavior on the flow. However, today the influence of hemodynamic parameters such as the shear rate on thrombotic reactions is not well understood. For the study of the shear-dependent mechanisms leading to the activation, adhesion and aggregation of platelets, a Couette flow system was used to investigate thrombocyte behavior with regard to well-defined flow conditions at shear-rate values between γ˙=400 $\dot \gamma = {\rm{400}}$ and 1400 1/s...
October 8, 2016: Biomedizinische Technik. Biomedical Engineering
Farzana Ferdous, Christopher Saski, William Bridges, Matthew Burns, Heather Dunn, Kathryn Elliott, Thomas R Scott
Thrombocytes are nucleated platelets involved in immune functions such as pathogen recognition and release of pro-inflammatory bioactive compounds when exposed to bacterial and viral molecules. However, the complete role of these cells in innate and adaptive immune responses is not understood, and little is known about their biology at the molecular-genetic level. Highly sensitive RNA-sequencing technologies were used to analyze the complete transcriptome of thrombocytes for the first time with analytical resolution focused on cell-based components of the immune system/response...
2016: PloS One
Irene Malkwitz, Angela Berndt, Runhui Zhang, Arwid Daugschies, Berit Bangoura
Toxoplasma (T.) gondii is able to infect various cell types in different hosts. The replication of this parasite within different peripheral mononuclear blood cell populations in chicken has not yet been fully understood. Aim of the present study was to investigate the impact of chicken erythrocytes and thrombocytes as potential host cells for T. gondii. Cultures of primary avian erythrocytes and thrombocytes were inoculated with tachyzoites of T. gondii type II strain ME49. Parasite replication was detected by a quantitative real-time PCR at different times postinoculation until 24 or 48 h, respectively, displaying long-term investigations for the chosen cultures...
October 3, 2016: Parasitology Research
A Stoll, A Pfitzner-Friedrich, F Reichmann, J Rauschendorfer, A Roessler, G Rademacher, G Knubben-Schweizer, C Sauter-Louis
Haemorrhagic diathesis (HD) in cattle is a relatively rare syndrome that can have many different causes. With the occurrence of bovine neonatal pancytopenia (BNP) in 2007, the number of cases of HD in cattle has increased. This led to an enhanced interest in diseases presenting with bleeding disorders. The possible causes of HD in cattle, the clinical findings, and the course of various diseases are described and evaluated. Furthermore, we determined whether cases of BNP occurred before the introduction of the vaccine Pregsure BVD since its widespread use was associated with the syndrome...
October 2016: Veterinary Journal
Christina Hart, Sabine Klatt, Johann Barop, Gunnar Mueller, Roland Schelker, Ernst Holler, Elisabeth Huber, Wolfgang Herr, Jochen Grassinger
Myelofibrosis is a myeloproliferative neoplasm that results in cytopenia, bone marrow fibrosis and extramedullary hematopoiesis. Allogeneic hematopoietic stem cell transplantation is the only curative treatment but is associated with a risk of delayed engraftment and graft failure. In this study patients with myelofibrosis (n=31) and acute myeloid leukemia (n=31) were analyzed for time to engraftment, graft failure and engraftment related factors. Early and late neutrophil engraftment and late thrombocyte engraftment was significantly delayed in patients with myelofibrosis as compared to acute myeloid leukemia and graft failure only occurred in myelofibrosis (6%)...
August 4, 2016: Haematologica
Numan Cim, Mertihan Kurdoglu, Serhat Ege, Ibrahim Yoruk, Gorkem Yaman, Recep Yildizhan
AIM: The aim of this study was to evaluate the roles of proangiogenic factors including serum vitamin D and vascular endothelial growth factor (VEGF) and anti-angiogenic factors including soluble endoglin (sEng) and soluble fms-like tyrosine kinase 1 (sFlt1) in the diagnosis and severity of late-onset preeclampsia. MATERIALS AND METHODS: The study was conducted at Yuzuncu Yil University Research and Education Hospital Department of Gynecology and Obstetrics. The study included a patient group of 40 women with late-onset preeclampsia who were pregnant at ≥32 weeks of gestation according to the last menstrual period (LMP) or ultrasonographic fetal biometric measurement and a control group of 40 healthy pregnant women who presented to our clinic for routine pregnancy examination and were at the same age and gestational period with those in the patient group...
September 23, 2016: Journal of Maternal-fetal & Neonatal Medicine
Daniel Brönnimann, Audrey Bouchet, Christoph Schneider, Marine Potez, Raphaël Serduc, Elke Bräuer-Krisch, Werner Graber, Stephan von Gunten, Jean Albert Laissue, Valentin Djonov
Our goal was the visualizing the vascular damage and acute inflammatory response to micro- and minibeam irradiation in vivo. Microbeam (MRT) and minibeam radiation therapies (MBRT) are tumor treatment approaches of potential clinical relevance, both consisting of parallel X-ray beams and allowing the delivery of thousands of Grays within tumors. We compared the effects of microbeams (25-100 μm wide) and minibeams (200-800 μm wide) on vasculature, inflammation and surrounding tissue changes during zebrafish caudal fin regeneration in vivo...
2016: Scientific Reports
Ashish H Shah, Richard A Anderson, Ali Raza Khan, Timothy D Kinnaird
Treating patients known to have immune thrombocytopenic purpura (ITP) presenting with acute coronary syndrome (ACS) pose challenges, especially if they undergo percutaneous coronary intervention and stenting, as they require certain period of dual anti-platelet medication based up on the type of stent been deployed. Co-existence of therapies to increase platelet number as well as anti-platelet efficacy at the same time appears contradictory; imbalance in antagonistic treatment approach of increasing platelet number to treat ITP and inhibiting their activity to treat ACS can result in life threatening complications...
August 20, 2016: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
M Żmigrodzka, M Guzera, A Miśkiewicz, D Jagielski, A Winnicka
Extracellular vesicles (EVs) are a heterogeneous group of structures which can be classified into smaller in size and relatively homogenous exosomes (EXSMs)-spherical fragments of lipid bilayers from inner cell compartments-and bigger in size ectosomes (ECSMs)-a direct consequence of cell-membrane blebbing. EVs can be found in body fluids of healthy individuals. Their number increases in cancer and other pathological conditions. EVs can originate from various cell types, including leukocytes, erythrocytes, thrombocytes, and neoplastic cells...
September 15, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Susann Rahmig, Romy Kronstein-Wiedemann, Juliane Fohgrub, Nicole Kronstein, Aleksandra Nevmerzhitskaya, Martin Bornhäuser, Max Gassmann, Alexander Platz, Rainer Ordemann, Torsten Tonn, Claudia Waskow
Human erythro-megakaryopoiesis does not occur in humanized mouse models, preventing the in vivo analysis of human hematopoietic stem cell (HSC) differentiation into these lineages in a surrogate host. Here we show that stably engrafted KIT-deficient NOD/SCID Il2rg(-/-)Kit(W41/W41) (NSGW41) mice support much improved human erythropoiesis and platelet formation compared with irradiated NSG recipients. Considerable numbers of human erythroblasts and mature thrombocytes are present in the bone marrow and blood, respectively...
October 11, 2016: Stem Cell Reports
Jose Hugo M Luz, Paula M Luz, Edson Marchiori, Leonardo A Rodrigues, Hugo R Gouveia, Henrique S Martin, Igor M Faria, Roberto R Souza, Roberto de Almeida Gil, Alexandre de M Palladino, Karina B Pimenta, Henrique S de Souza
Systemic chemotherapy treatments, commonly those that comprise oxaliplatin, have been linked to the appearance of distinctive liver lesions that evolves to portal hypertension, spleen enlargement, platelets sequestration, and thrombocytopenia. This outcome can interrupt treatment or force dosage reduction, decreasing efficiency of cancer therapy. We conducted a prospective phase II study for the evaluation of partial splenic embolization in patients with thrombocytopenia that impeded systemic chemotherapy continuation...
September 9, 2016: Cancer Medicine
Irfan Zia Qureshi, Asia Bibi, Sana Shahid, Madiha Ghazanfar
Use of pesticides or insecticides can be highly toxic to aquatic life forms due to leaching and agricultural runoff, rains or flood. Fipronil (FP) is a GABA receptor inhibitor, while buprofezin (BPFN) is an insect growth regulator. Presently, we exposed groups of aquaria acclimated carp fish (Cyprinus carpio) for 96h to sub-lethal concentrations of fipronil (400μgL(-1); 9.15×10(-7)molL(-1)) and buprofezin (BPFN, 100mgL(-1); 1.072×10(-6)molL(-1)) singly or in combination. The extent of damage was assessed at biochemical, hematological, molecular biological and histopathological level...
October 2016: Aquatic Toxicology
Lincoln Lima Corrêa, Marcos Sidney Brito Oliveira, Marcos Tavares-Dias, Paulo Sérgio Ceccarelli
Among Kinetoplastida, the Trypanosoma is the genus with the highest occurrence infecting populations of marine fish and freshwater in the world, with high levels of prevalence, causing influences fish health and consequent economic losses, mainly for fish populations in situation stress. This study investigated infections of Hypostomus spp. by Trypanosoma spp. and leeches, as well as blood parameters of this host in the network of tributaries of the Tapajós River in the state of Pará, in the eastern Amazon region in Brazil...
July 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
Ibrahim Sehitoglu, Recep Bedir, Erkan Cure, Medine Cumhur Cure, Suleyman Yuce, Nursel Dilek
BACKGROUND: c-Kit is a proto-oncogene that encodes tyrosine kinase receptor (CD117). Mean platelet volume (MPV) is a useful marker, providing information on platelet function and diameter. OBJECTIVE: To investigate c-Kit expression and intensity in patients with Kaposi's sarcoma (KS) and to investigate the relation between Ki-67 proliferation and MPV. METHODS: A total of 32 patients, diagnosed with classic cutaneous KS, were included in this study...
July 2016: Anais Brasileiros de Dermatologia
M Niedzwiecki, Y Yamada, I Inci, W Weder, W Jungraithmayr
BACKGROUND: Allergies are hypersensitive reactions of the immune system on antigen exposure similar to immune reactions after transplantation (Tx). Their activity can change after Tx. The lung as a transplantable organ is challenged two-fold, by antigens from the blood and the air environment. Herein we analyzed if airway allergies change after lung Tx. METHODS: We systematically reviewed patients' airway allergies before and after lung Tx between 1992 and 2014...
July 2016: Transplantation Proceedings
Ferdinand I Broekema, Wim van Oeveren, Andrea Boerendonk, Prashant K Sharma, Rudolf R M Bos
BACKGROUND: For most topical hemostatic agents the mechanism of hemostatic action is not fully understood. OBJECTIVE: This work aimed to investigate the hemostatic mechanism of action and viscoelastic properties of polyurethane foam (PU) in comparison to the widely used collagen and gelatin. METHODS: The hemostatic mechanism of action of the materials was tested using human whole blood and platelet-poor plasma (PPP). The ability of the hemostatic agent to exert pressure on the wound was quantified in terms of its viscoelastic properties both under dry and wet conditions using a low load compression tester (LLCT)...
August 12, 2016: Bio-medical Materials and Engineering
María J Forzán, Todd G Smith, Raphaël V Vanderstichel, Natacha S Hogan, Cornelia V Gilroy
BACKGROUND: Although the Wood Frog, Rana sylvatica, is used in research on infectious diseases of amphibians, hematologic RIs or response to infection have not been established. OBJECTIVES: The purpose of the study was to determine hematologic RIs for adult Wood Frogs and alterations associated with infection with Frog Virus 3 (FV3, Ranavirus sp.). METHODS: Blood was collected from 40 wild-caught adult Wood Frogs that had been in captivity for 6 months...
September 2016: Veterinary Clinical Pathology
Tomi Virtanen, Saana Eskelinen, Eila Sailas, Jaana Suvisaari
Constipation and dyspepsia are disturbing gastrointestinal symptoms that are often ignored in research on physical comorbidities of schizophrenia. The aim was to assess dyspepsia and constipation in a sample of outpatients with schizophrenia spectrum psychoses. A general practitioner performed a thorough physical health check for 275 outpatients and diagnosed constipation and dyspepsia. This study assessed the possible contribution of several sociodemographic, lifestyle, and clinical variables to constipation and dyspepsia using logistic regression analysis...
August 26, 2016: Nordic Journal of Psychiatry
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