keyword
https://read.qxmd.com/read/38546837/predicting-deamidation-and-isomerization-sites-in-therapeutic-antibodies-using-structure-based-in-silico-approaches
#1
JOURNAL ARTICLE
David Hoffmann, Joschka Bauer, Markus Kossner, Andrew Henry, Anne R Karow-Zwick, Giuseppe Licari
Asparagine (Asn) deamidation and aspartic acid (Asp) isomerization are common degradation pathways that affect the stability of therapeutic antibodies. These modifications can pose a significant challenge in the development of biopharmaceuticals. As such, the early engineering and selection of chemically stable monoclonal antibodies (mAbs) can substantially mitigate the risk of subsequent failure. In this study, we introduce a novel in silico approach for predicting deamidation and isomerization sites in therapeutic antibodies by analyzing the structural environment surrounding asparagine and aspartate residues...
2024: MAbs
https://read.qxmd.com/read/38546233/hydrogen-treatment-reduces-electroencephalographic-activity-and-neuronal-death-in-rats-with-refractory-status-epilepticus-by-inhibiting-membrane-nr2b-phosphorylation-and-oxidative-stress
#2
JOURNAL ARTICLE
Ruihua Jia, Gemin Zhu, Rui Zhao, Tian Li, Wen Jiang, Xiaoli Cui
OBJECTIVE: To investigate the effects of hydrogen therapy on epileptic seizures in rats with refractory status epilepticus and the underlying mechanisms. METHODS: Status epilepticus was induced using pilocarpine. The effects of hydrogen treatment on epilepsy severity in model rats were then monitored using Racine scores and electroencephalography (EEG), followed by western blot of plasma membrane N-methyl-D-aspartate receptor subtype 2B (NR2B) and phosphorylated NR2B expression...
March 2024: Journal of International Medical Research
https://read.qxmd.com/read/38546139/elevated-liver-enzymes-in-the-first-trimester-are-associated-with-gestational-diabetes-mellitus-a-prospective-cohort-study
#3
JOURNAL ARTICLE
Lingling Cui, Xiaoli Yang, Zhiqian Li, Yuting Gao, Zhengya Zhang, Dongmei Xu, Xinxin Liu
AIMS: Previous studies have found that a single liver enzyme may predict gestational diabetes mellitus (GDM), but the results have been inconsistent. This study aimed to explore the associations of liver enzymes in early pregnancy with risk of GDM, as well as to independently rank risk factors. METHODS: This prospective cohort study included 1295 women who underwent liver enzyme measurements during early pregnancy and completed GDM assessment in mid-pregnancy. Logistic regression and restricted cubic spline analyses were conducted to assess the relationship between liver enzymes and risk of GDM...
May 2024: Diabetes/metabolism Research and Reviews
https://read.qxmd.com/read/38545725/the-effects-of-high-intensity-interval-training-and-iranian-propolis-extract-on-serum-levels-of-trpv4-and-cyp2e1-proteins-in-patients-with-nonalcoholic-fatty-liver
#4
JOURNAL ARTICLE
Khadijeh Irandoust, Mahsa Gholamhosseini, Rasool Samimi, Ismail Dergaa, Helmi Ben Saad, Mortez Taheri
INTRODUCTION: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver failure, fibrosis, cirrhosis, and liver cancer, which can eventually lead to death. AIM: To investigate the effects of high-intensity interval training (HIIT) and iranian propolis extract on serum levels of transient receptor potential cation channel subfamily V member 4 (TRPV4) and cytochrome P450 2E1 (CYP2E1) proteins in patients with NAFLD. METHODS: Thirty-two patients with NAFLD (mean±standard deviation of age: 45...
January 5, 2024: La Tunisie Médicale
https://read.qxmd.com/read/38545550/the-protective-effect-of-traditional-chinese-medicine-jinteng-qingbi-granules-on-rats-with-rheumatoid-arthritis
#5
JOURNAL ARTICLE
Yingying Wan, Wenting Sun, Jiaxi Yang, Haonan Wang, Wenqian Wang, Wanting Ye, Guoliang Cheng, Bing Li, Jianxun Ren, Qiuai Kou
Introduction: Jinteng Qingbi granules (JTQBG), a traditional Chinese medicine formulation, are widely used for the treatment of rheumatoid arthritis (RA) due to their satisfactory therapeutic efficacy. However, the underlying mechanism of action remains unclear. This study aims to investigate the protective effects of JTQBG against RA and elucidates its potential molecular mechanisms. Methods: A collagen-induced arthritis (CIA) rat model was utilized, and JTQBG (1.25, 2.5, 5 g/kg/day) or methotrexate (MTX, 1 mg/kg/week) was orally administered...
2024: Frontiers in Pharmacology
https://read.qxmd.com/read/38545173/effect-of-hyperbaric-oxygen-treatment-on-patients-with-heatstroke-complicated-by-multiple-organ-dysfunction-a-retrospective-study
#6
JOURNAL ARTICLE
Xiao-Xiao Ni, Nian-Bo He, Ye-Qun Guo, Yi-Xuan Dou, Xiao-Juan Xie, Zhi-Feng Liu
BACKGROUND: The benefits of hyperbaric oxygen (HBO) in treating animals with heat stroke (HS) have been established. This study aims to retrospectively analyze the effect of HBO on multiple organ dysfunction following HS in humans. METHODS: Retrospective data were collected from patients with HS admitted to our hospital in the past 7 years. Patients were categorized into groups based on whether they received HBO therapy. The study compared various factors, including sequential organ failure assessment (SOFA) and acute physiology and chronic health evaluation-Ⅱ (APACHE-Ⅱ) scores, mortality rates, neurological function scores, serum myocardial enzyme levels, liver, kidney, and coagulation function indicators, blood routine results, electrolyte levels, and modified Barthel index (MBI) score for standard daily living ability before treatment and after 2 and 4 weeks of treatment...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38544644/use-of-ozone-therapy-and-thymoquinone-in-the-prevention-of-formaldehyde-toxicity-by-inhalation-an-experimental-study
#7
JOURNAL ARTICLE
Feyza Aksu, Ahmet Kavaklı, Tuncay Kuloglu, Seval Yilmaz, Emre Kaya, Ramazan Fazil Akkoc, Mustafa Yilmaz, Elif Emre, Murat Ogetürk
INTRODUCTION: The study determined the damage caused by formaldehyde (FA) exposure in blood and liver samples using biochemical markers. Histopathological analysis was performed using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method and measurement of CD68 cell density. To what extent the antioxidant molecules thymoquinone (TQ) and ozone (O3 ) reversed the damage caused by FA exposure was investigated, both when used alone and combined. METHODS: Fifty-six Sprague-Dawley male rats of eight to ten weeks of age were used in the experiment...
February 2024: Curēus
https://read.qxmd.com/read/38543737/effects-of-hcv-clearance-with-direct-acting-antivirals-daas-on-liver-stiffness-liver-fibrosis-stage-and-metabolic-cellular-parameters
#8
JOURNAL ARTICLE
Joana Ferreira, Manuel Bicho, Fátima Serejo
INTRODUCTION: Chronic hepatitis C (CHC) is a clinical and pathological syndrome with various causes and is characterized by varying degrees of hepatocellular necrosis and inflammation. It is a significant cause of liver transplantation and liver-related death worldwide. The hepatic manifestations of CHC are typically characterized by slowly progressing liver fibrosis, which is a non-specific and often disproportionate response to tissue damage. A large majority of HCV patients have extrahepatic manifestations with varying degrees of severity...
February 27, 2024: Viruses
https://read.qxmd.com/read/38543657/engineering-a-lactobacillus-lysine-riboswitch-to-dynamically-control-metabolic-pathways-for-lysine-production-in-corynebacterium-glutamicum
#9
JOURNAL ARTICLE
Qingwei Jiang, Feng Geng, Juan Shen, Ping Zhu, Zhaoxin Lu, Libang Zhou, Fengxia Lu
Knock-out of genes of metabolic pathways is conventionally used in the metabolic engineering of microorganisms, but it is not applicable for genes of essential pathways. In order to avoid undesirable effects caused by gene deletion, it is attractive to develop riboswitches to dynamically control the metabolic pathways of microbial cell factories. In this regard, the aim of this study is to utilize the lysine riboswitch to control gene expressions of the biosynthetic pathways and by-pathways and thus improve lysine production in Corynebacterium glutamicum ...
March 18, 2024: Microorganisms
https://read.qxmd.com/read/38543575/-candida-dubliniensis-in-japanese-oral-microbiota-a-cross-sectional-study-of-six-geographic-regions-in-japan
#10
JOURNAL ARTICLE
Tomoko Ohshima, Yoko Mukai, Hitoshi Watanabe, Keijiro Ohshima, Koichi Makimura, Takashi Komabayashi, Chul Ahn, Karen Meyer, Nobuko Maeda
INTRODUCTION: Candida dubliniensis was reclassified from the C. albicans genotype D, and reports show its frequent detection in HIV-positive individuals and easy acquisition of antifungal drug resistance. However, the oral carriage rate in healthy people and contribution to candidiasis in Japan is unclear. METHODS: We conducted a cross-sectional survey of the C. dubliniensis carriage rate, performed genotyping and tested antifungal drug susceptibility and protease productivity...
March 5, 2024: Microorganisms
https://read.qxmd.com/read/38543475/the-gut-microbiome-in-the-first-one-thousand-days-of-neurodevelopment-a-systematic-review-from-the-microbiome-perspective
#11
REVIEW
Nathalia F Naspolini, Paulo A Schüroff, Maria J Figueiredo, Gabriela E Sbardellotto, Frederico R Ferreira, Daniel Fatori, Guilherme V Polanczyk, Alline C Campos, Carla R Taddei
Evidence shows that the gut microbiome in early life is an essential modulator of physiological processes related to healthy brain development, as well as mental and neurodegenerative disorders. Here, we conduct a systematic review of gut microbiome assessments on infants (both healthy and with conditions that affect brain development) during the first thousand days of life, associated with neurodevelopmental outcomes, with the aim of investigating key microbiome players and mechanisms through which the gut microbiome affects the brain...
February 20, 2024: Microorganisms
https://read.qxmd.com/read/38543109/long-term-supplementation-of-royal-jelly-raydel-%C3%A2-improves-zebrafish-growth-embryo-production-and-survivability-blood-lipid-profile-and-functionality-of-vital-organs-a-72-weeks-consumption-study
#12
JOURNAL ARTICLE
Kyung-Hyun Cho, Hyo-Seon Nam, Ashutosh Bahuguna, Ji-Eun Kim
Royal jelly is a honeybee product with substantial pharmacological and health promotional activities. Nevertheless, the health implications associated with the prolonged dietary supplementation of royal jelly have yet to be elucidated extensively. Herein, 72 weeks of dietary supplementation of royal jelly at 5% and 10% ( w / w ) were investigated to assess the impact on zebrafish survivability, body weight, liver, testis, ovary functionality, and blood lipid profile. The results revealed no adverse effect of 72 weeks of royal jelly supplementation on zebrafish survivability...
March 1, 2024: Pharmaceuticals
https://read.qxmd.com/read/38543063/pharmakokinetics-of-mistletoe-lectins-after-intravenous-application-of-a-mistletoe-product-in-healthy-subjects
#13
JOURNAL ARTICLE
Ann-Kathrin Lederer, Sabine Rieger, Michael Schink, Roman Huber
Mistletoe lectins (ML) have cytotoxic and immunomodulating properties, and subcutaneously applied mistletoe products (MP) containing ML have approval for supportive cancer treatment. MP are also given off-label intravenously, but data about pharmacokinetics are widely lacking. Therefore, the aim of our phase I trial was to evaluate the pharmacokinetics and safety of intravenously applied natural ML. Initially, 12 healthy male volunteers were planned to receive a single infusion of 2000 mg Helixor® P. We had to terminate the study prematurely after the inclusion of eight subjects due to elevation of all subjects' liver enzymes...
February 22, 2024: Pharmaceuticals
https://read.qxmd.com/read/38542768/the-effects-of-olive-oil-consumption-on-biochemical-parameters-and-body-mass-index-of-people-with-nonalcoholic-fatty-liver-disease-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#14
REVIEW
Georgios Tsamos, Georgios Kalopitas, Kleo Evripidou, Dimitra Vasdeki, Theocharis Koufakis, Vasileios Kanavas, Christina Antza, Georgios Germanidis, Michail Chourdakis
Nonalcoholic fatty liver disease (NAFLD), the most common chronic liver disorder, is closely associated with insulin resistance, obesity, and metabolic syndromes. A body of research has proposed that olive oil, a basic component of the Mediterranean diet with antioxidant and anti-inflammatory properties, may alleviate metabolic disturbances and retard the progression of NAFLD. We conducted a systematic review and meta-analysis to assess the effectiveness of olive oil intake in people with NAFLD. We systematically searched the major electronic databases (PubMed/MEDLINE, Scopus, Cochrane Central Register of Controlled Trials), as well as grey literature sources, to identify randomized controlled trials (RCTs) investigating the effects of olive oil consumption on biochemical and anthropometric parameters of individuals with NAFLD...
March 15, 2024: Nutrients
https://read.qxmd.com/read/38541661/influence-of-lemongrass-essential-oil-cymbopogon-flexuosus-supplementation-on-diabetes-in-rat-model
#15
JOURNAL ARTICLE
Ailton S S Júnior, Felipe J Aidar, Luiz A S Silva, Thais de B Silva, Sara F M de Almeida, Daiane C S Teles, Waldecy de L Junior, Dulce M Schimieguel, Daniel A de Souza, Ana C S Nascimento, Enilton A Camargo, Jymmys L Dos Santos, Ana M de O E Silva, Rogéria de S Nunes, Lysandro P Borges, Ana A M Lira
(1) Background: Species of the genus Cymbopogon and its essential oil are known for their antioxidant and hypoglycemic effects. This study aimed to investigate the impact of the essential oil of Cymbopogon flexuosus (EOCF), and its major component, citral, on glycemic, lipid, antioxidant parameters, and oxidative stress in a type 1 diabetes (DM1) rat model. (2) Methods: Initially, EOCF was analyzed by Gas chromatography-mass spectrometry (GC-MS) and the antioxidant activity of EOCF and citral was evaluated...
March 4, 2024: Life
https://read.qxmd.com/read/38541107/alcoholic-liver-disease-nonalcoholic-fatty-liver-disease-index-at-diagnosis-is-associated-with-all-cause-mortality-during-follow-up-in-patients-with-antineutrophil-cytoplasmic-antibody-associated-vasculitis
#16
JOURNAL ARTICLE
Minsuk Cho, Woongchan Rah, Jason Jungsik Song, Yong-Beom Park, Sang-Won Lee
Background and Objectives : The purpose of this study was to investigate whether a new index related to chronic liver disease, the alcoholic liver disease/nonalcoholic fatty liver disease index (ANI) at diagnosis, is associated with all-cause mortality during follow-up in patients with antineutrophil cytoplasmic antibody-associated vasculitis (AAV). Materials and Methods : In this study, we included 270 patients with AAV. ANI was calculated using the following equation: ANI = -58.5 + 0.637 (adjusted mean corpuscular volume) + 3...
February 24, 2024: Medicina
https://read.qxmd.com/read/38540788/ammoniagenic-action-of-valproate-without-signs-of-hepatic-dysfunction-in-rats-possible-causes-and-supporting-evidence
#17
JOURNAL ARTICLE
Gubidat Alilova, Lyudmila Tikhonova, Carmina Montoliu, Elena Kosenko
(1) Background: Valproic acid (VPA) is one of the frequently prescribed antiepileptic drugs and is generally considered well tolerated. However, VPA neurologic adverse effects in the absence of liver failure are fairly common, suggesting that in the mechanism for the development of VPA-induced encephalopathy, much more is involved than merely the exposure to hyperammonemia (HA) caused by liver insufficiency to perform detoxification. Taking into account the importance of the relationship between an impaired brain energy metabolism and elevated ammonia production, and based on the ability of VPA to interfere with neuronal oxidative pathways, the current study intended to investigate a potential regional ammoniagenic effect of VPA on rats' brains by determining activities of the enzymes responsible for ammonia production and neutralization...
March 19, 2024: Biomolecules
https://read.qxmd.com/read/38540717/comparison-between-the-regenerative-and-therapeutic-impacts-of-bone-marrow-mesenchymal-stem-cells-and-adipose-mesenchymal-stem-cells-pre-treated-with-melatonin-on-liver-fibrosis
#18
JOURNAL ARTICLE
Ahmed Elzainy, Abir El Sadik, Waleed Mohammad Altowayan
BACKGROUND: The distinctive feature of liver fibrosis is the progressive replacement of healthy hepatic cells by the extracellular matrix protein, which is abundant in collagen I and III, with impaired matrix remodeling. The activation of myofibroblastic cells enhances the fibrogenic response of complex interactions of hepatic stellate cells, fibroblasts, and inflammatory cells to produce the excessive deposition of the extracellular protein matrix. This process is activated by multiple fibrogenic mediators and cytokines, such as TNF-α and IL-1β, accompanied with a decrease in the anti-fibrogenic factor NF-κβ...
March 1, 2024: Biomolecules
https://read.qxmd.com/read/38539976/dietary-25-hydroxycholecalciferol-supplementation-as-a-vitamin-d-3-substitute-improves-performance-egg-quality-blood-indexes-jejunal-morphology-and-tibia-quality-in-late-phase-laying-hens
#19
JOURNAL ARTICLE
Shan Gao, Kai Qiu, Junjie Zheng, Haijun Zhang, Jing Wang, Xiaolong Qi, Shugeng Wu
This study aimed to investigate whether a dietary 25-OHD3 addition improved the performance, egg quality, blood indexes, antioxidant status, jejunal morphology, and tibia quality of aged laying hens compared to a dietary VD3 addition. A total of 270 Hy-Line Brown laying hens at 55 wk of age were randomly assigned to three dietary treatments with six replicates (15 birds per replicate with 3 birds per cage). Chickens were fed a corn-soybean meal diet supplementation of 4000 IU/kg VD3 (control group), 50 μg/kg 25-OHD3 and 2000 IU/kg VD3 (experimental group 1), or 50 μg/kg 25-OHD3 and 4000 IU/kg VD3 (experimental group 2) for 12 weeks...
March 13, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38539966/the-substitution-of-fishmeal-with-yeast-culture-in-the-yellow-catfish-pelteobagrus-fulvidraco-diet-growth-serum-biochemical-indices-and-intestinal-and-hepatopancreatic-histology
#20
JOURNAL ARTICLE
Hongfei Huang, Xiaoqin Li, Beibei Guo, Yugui Zhang, Xu Yang, Yan Liu, Xiangjun Leng
Yeast culture is a complex fermentation product consisting of fermentation substrate, yeast cells and their metabolites. This study investigated the potential of yeast culture in replacing fishmeal in the diet of yellow catfish ( Pelteobagrus fulvidraco ). First, a basal diet was formulated to contain 160 g/kg fishmeal (CON), and then the dietary fishmeal was decreased to 120, 80, 40 and 0 g/kg via yeast culture inclusion, respectively, to form another four isonitrogenous and isolipidic diets (YC-12, YC-8, YC-4 and YC-0)...
March 12, 2024: Animals: An Open Access Journal From MDPI
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