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Cold inducible rna binding protein

K Yang, Q Zhang, Z Wen, Y Pan, S Yu, J He, X Yang, P Liu, Y Cui
Cold-inducible RNA binding protein (CIRP) is over-expressed during cold and many other stresses, and could regulate the adaptation to hypothermia. In the present investigation, the objective was to determine the expression of CIRP in adult yak heart, liver, spleen, lung, kidney, brain, ovary, testis and skin by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry from mRNA and protein levels. The CIRP open reading frame encoding was cloned from the domestic yak brain...
2016: Folia Morphologica (Warsz)
Carla B Green
In mammals, rhythms in body temperature help to entrain and synchronize circadian rhythms throughout the organism, and the cold-inducible RNA-binding protein (CIRBP) is one of the mediators of these daily temperature changes. Cirbp mRNA expression is regulated by the daily subtle rhythms in body temperature, and a new study by Gotic and colleagues (pp. 2005-2017) reveals a surprising and novel mechanism that involves temperature-dependent enhancement of splicing efficiency.
September 1, 2016: Genes & Development
Ivana Gotic, Saeed Omidi, Fabienne Fleury-Olela, Nacho Molina, Felix Naef, Ueli Schibler
In mammals, body temperature fluctuates diurnally around a mean value of 36°C-37°C. Despite the small differences between minimal and maximal values, body temperature rhythms can drive robust cycles in gene expression in cultured cells and, likely, animals. Here we studied the mechanisms responsible for the temperature-dependent expression of cold-inducible RNA-binding protein (CIRBP). In NIH3T3 fibroblasts exposed to simulated mouse body temperature cycles, Cirbp mRNA oscillates about threefold in abundance, as it does in mouse livers...
September 1, 2016: Genes & Development
Alexandra C Bolognese, Archna Sharma, Weng-Lang Yang, Jeffrey Nicastro, Gene F Coppa, Ping Wang
Cold-inducible RNA-binding protein (CIRP) is a novel inflammatory mediator that stimulates the release of proinflammatory cytokines from macrophages in sepsis. Given the immune dysregulation that characterizes sepsis, the effect of CIRP on other immune cells is an area of increasing interest that has not yet been studied. In the present study, we hypothesized that extracellular CIRP promotes activation of T lymphocytes in the spleen during sepsis. We observed that mice subjected to sepsis by cecal ligation and puncture showed significantly higher expression of the early activation markers CD69 and CD25 at 20 h on CD4(+) splenic T cells, and significantly higher CD69 expression on CD8(+) splenic T cells compared with sham-operated controls...
August 29, 2016: Cellular & Molecular Immunology
Ignacio M Larrayoz, Manuel Rey-Funes, Daniela S Contartese, Federico Rolón, Anibal Sarotto, Veronica B Dorfman, Cesar F Loidl, Alfredo Martínez
Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mammalian retina is so far unknown. Here we show the effects of hypothermia on the expression of these CSPs in retina-derived cell lines and in the retina of newborn and adult rats. Two cell lines of retinal origin, R28 and mRPE, were exposed to 32°C for different time periods and CSP expression was measured by qRT-PCR and Western blotting...
2016: PloS One
Yang Juan, Wu Haiqiao, Wenyao Xie, Huang Huaping, Han Zhong, Zhou Xiangdong, Victor P Kolosov, Juliy M Perelman
Acute or chronic cold exposure exacerbates chronic inflammatory airway diseases, such as chronic obstructive pulmonary disease (COPD) and asthma. Cold-inducible RNA-binding protein (CIRP) is a cold-shock protein and is induced by various environmental stressors, such as hypothermia and hypoxia. In this study, we showed that CIRP gene and protein levels were significantly increased in patients with COPD and in rats with chronic airway inflammation compared with healthy subjects. Similarly, inflammatory cytokine production and MUC5AC secretion were up-regulated in rats following cigarette smoke inhalation...
September 2016: International Journal of Biochemistry & Cell Biology
Rie Hayashi, Chieko Sugita, Mamoru Sugita
The unicellular cyanobacterium Synechococcus elongatus has three RNA-binding protein (Rbp) genes, rbp1, rbp2 and rbp3. The rbp1 gene was upregulated by cold treatment while rbp2 and rbp3 expression decreased remarkably after exposure to cold temperatures. To investigate the mechanism underlying cold-induced rbp1 expression, a series of rbp1-luxAB transcriptional fusion constructs were expressed in S. elongatus PCC 7942 under cold conditions. The results showed that the region from -33 to -3 of the transcription initiation site contains an essential sequence for basal transcription of the rbp1 gene and that the 120-bp region (-34 to -153) does not contain critical cis-elements required for cold-shock induction...
January 2017: Archives of Microbiology
Lingxiu Chen, Danhua Ran, Wenyue Xie, Qing Xu, Xiangdong Zhou
Mucus overproduction is an important feature in patients with chronic inflammatory airway diseases and cold air stimulation has been shown to be associated with the severity of these diseases. However, the regulatory mechanisms that mediate excessive mucin production under cold stress remain elusive. Recently, the cold-inducible RNA-binding protein (CIRP) has been shown to be markedly induced after exposure to cold air. In this study, we sought to explore the expression of CIRP within bronchial biopsy specimens, the effect on mucin5AC (MUC5AC) production in chronic inflammatory airway diseases and the potential signaling pathways involved in cold air stimulation process...
October 2016: International Immunopharmacology
Ling He, Meixia Fang, Liguo Chen, Jianhua Zhou, Jing Yuan, Jing Xu, Yan Shan, Qingyun Xu, Tingting Xiong
OBJECTIVE: To screen for mRNAs associated with blood stasis syndrome and to explore the genetic mechanisms of blood stasis syndrome in hypertension. METHODS: This study involved groups of patients with hypertension and blood stasis, including those with Qi deficiency, Qi stagnation, cold retention and heat retention; as well as hypertensive patients without blood stasis and healthy individuals. Human umbilical vein endothelial cells were co-cultured with the sera of these healthy individuals and patients with blood stasis syndrome...
April 2016: Journal of Traditional Chinese Medicine, Chung i Tsa Chih Ying Wen Pan
Hae Na Lee, Sung-Min Ahn, Ho Hee Jang
Transforming growth factor-β1 (TGF-β1), a potent inducer of epithelial-to-mesenchymal transition (EMT), upregulates the cold-inducible RNA-binding protein (CIRP). The link between CIRP and EMT, however, remains unknown. To determine the role of CIRP in EMT, we performed CIRP knockdown and overexpression experiments in in vitro TGF-β1-induced EMT models. We found that CIRP overexpression promoted the downregulation of epithelial markers and the upregulation of mesenchymal markers after TGF-β1 treatment for EMT induction...
September 2, 2016: Biochemical and Biophysical Research Communications
Heyam I M Al-Astal, Maram Massad, Manaf AlMatar, Harith Ekal
Cell rapidly adapts to the harsh environment under certain conditions such as hypothermia and stress via activity of certain specific cold-shock proteins. Two cold shock proteins, RNA-binding motif protein 3 (RBM3) and coldinducible RNA binding proteins (CIRP), have been unveiled in human cells. RBM3, which is able to regulate their expression at the level of translation by binding to different transcripts, allows the cell to respond rapidly to environmental signals. Additionally, expression of RBM3 is induced under mild to moderate hypothermia, and stress...
2016: Protein and Peptide Letters
Guan Wang, Jian-Ning Zhang, Jia-Kui Guo, Ying Cai, Hong-Sheng Sun, Kun Dong, Cheng-Gang Wu
Cold-inducible RNA-binding protein (CIRP), a key regulatory protein, could be facilitated by mild hypothermia in the brain, heart and liver. This study observed the effects of mild hypothermia at 31 ± 0.5°C on traumatic brain injury in rats. Results demonstrated that mild hypothermia suppressed apoptosis in the cortex, hippocampus and hypothalamus, facilitated CIRP mRNA and protein expression in these regions, especially in the hypothalamus. The anti-apoptotic effect of mild hypothermia disappeared after CIRP silencing...
May 2016: Neural Regeneration Research
In Seol Yoo, Sun Young Lee, Chan Keol Park, Jeong Chan Lee, Young Kim, Su Jin Yoo, Seung Cheol Shim, Yoon Seok Choi, Young Lee, Seong Wook Kang
AIM: There is growing evidence that cold-inducible RNA-binding protein (CIRP) promotes inflammatory responses. This study investigated the relationship between CIRP and rheumatoid arthritis (RA). METHODS: Peripheral blood and synovial fluid were collected from 15 patients with RA and from 16 patients with osteoarthritis (OA). The concentration of CIRP was measured with the sandwich enzyme-linked immunosorbent assay (ELISA). RESULTS: The concentration of serum CIRP was significantly elevated in the RA patient group (RA patients = 26...
June 17, 2016: International Journal of Rheumatic Diseases
Mathieu Beauchemin, Sougata Roy, Sarah Pelletier, Alexandra Averback, Frederic Lanthier, David Morse
Roughly two-thirds of the proteins annotated as transcription factors in dinoflagellate transcriptomes are cold shock domain-containing proteins (CSPs), an uncommon condition in eukaryotic organisms. However, no functional analysis has ever been reported for a dinoflagellate CSP, and so it is not known if they do in fact act as transcription factors. We describe here some of the properties of two CSPs from the dinoflagellate Lingulodinium polyedrum, LpCSP1 and LpCSP2, which contain a glycine-rich C-terminal domain and an N-terminal cold shock domain phylogenetically related to those in bacteria...
January 2016: MSphere
Cindy Cen, Weng-Lang Yang, Hao-Ting Yen, Jeffrey M Nicastro, Gene F Coppa, Ping Wang
BACKGROUND: Renal ischemia-reperfusion injury, commonly caused by major operation and shock, leads to acute kidney injury and is associated with high morbidity and mortality. Cold-inducible ribonucleic acid-binding protein, a cold shock protein, has recently been identified as a damage-associated molecular pattern. We hypothesized that cold-inducible ribonucleic acid-binding protein exacerbates severity of injury in renal ischemia-reperfusion. METHODS: Renal ischemia was induced in an 8-week-old male C57BL/6 wild-type mice and Cirp(-/-) mice via bilateral clamping of renal pedicles for 30 minutes, followed by reperfusion for 5 or 24 hours and harvest of blood and renal tissue for analysis...
August 2016: Surgery
Weng-Lang Yang, Archna Sharma, Zhimin Wang, Zhigang Li, Jie Fan, Ping Wang
Cold-inducible RNA-binding protein (CIRP) is a damage-associated molecular pattern (DAMP) molecule which stimulates proinflammatory cytokine release in hemorrhage and sepsis. Under these medical conditions, disruption of endothelial homeostasis and barrier integrity, typically induced by proinflammatory cytokines, is an important factor contributing to morbidity and mortality. However, the role of CIRP in causing endothelial dysfunction has not been investigated. In this study, we show that intravenous injection of recombinant murine CIRP (rmCIRP) in C57BL/6 mice causes lung injury, evidenced by vascular leakage, edema, increased leukocyte infiltration and cytokine production in the lung tissue...
May 24, 2016: Scientific Reports
DanHua Ran, LingXiu Chen, WenYue Xie, Qing Xu, Zhong Han, HuaPing Huang, XiangDong Zhou
Mucus overproduction is an important manifestation of chronic airway inflammatory diseases, however, the mechanisms underlying the association between cold air and mucus overproduction remain unknown. We found that the expression of the cold-inducible RNA binding protein (CIRP) was increased in patients with chronic obstructive pulmonary disease (COPD). In the present study, we tested whether CIRP was involved in inflammatory factors and mucin5AC (MUC5AC) expression after cold stimulation and investigated the potential signaling pathways involved in this process...
August 1, 2016: Archives of Biochemistry and Biophysics
Xinzhou Zhu, Christoph Bührer, Sven Wellmann
Cold-inducible RNA-binding protein (CIRP) and RNA-binding motif protein 3 (RBM3) are two evolutionarily conserved RNA-binding proteins that are transcriptionally upregulated in response to low temperature. Featuring an RNA-recognition motif (RRM) and an arginine-glycine-rich (RGG) domain, these proteins display many similarities and specific disparities in the regulation of numerous molecular and cellular events. The resistance to serum withdrawal, endoplasmic reticulum stress, or other harsh conditions conferred by RBM3 has led to its reputation as a survival gene...
October 2016: Cellular and Molecular Life Sciences: CMLS
Lin Wu, He-Liang Sun, Yu Gao, Kang-Li Hui, Miao-Miao Xu, Hao Zhong, Man-Lin Duan
Therapeutic hypothermia is well known for its protective effect against brain injury after cardiac arrest, but the exact mechanism remains unclear. Cold-inducible RNA-binding protein (CIRP), a member of cold shock protein, enables mammalian cells to withstand decreased temperature by regulating gene translation. However, the role of CIRP in global cerebral ischemia after therapeutic hypothermia has not been clearly elucidated. In the present study, rats resuscitated from 4 min of cardiac arrest were separately treated with therapeutic hypothermia (immediately after return of spontaneous circulation (ROSC); targeted temperature at 33 °C) and therapeutic normothermia (targeted temperature at 36...
March 19, 2016: Molecular Neurobiology
Gang Li, Le Yang, Hai Yuan, Yang Liu, Yuxiang He, Xuejun Wu, Xing Jin
BACKGROUND: Cold-inducible RNA-binding protein (CIRP) is a recently identified proinflammatory cytokine. We hypothesize that CIRP is involved in the progression of abdominal aortic aneurysms (AAA) and that anti-CIRP treatment could inhibit this progression. METHODS: We investigated CIRP expression in the sera and aneurysmal tissues of human AAA patients and elastase-induced AAA rats. To further examine the role of CIRP in the development of AAA, anti-CIRP antibody (1 mg/kg) or nonimmunized control immunoglobulin (Ig)G (1 mg/kg) was injected via the caudal vein in the experimental AAA model...
June 2016: Surgery
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