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https://www.readbyqxmd.com/read/27827841/leaf-volatile-compounds-and-associated-gene-expression-during-short-term-nitrogen-deficient-treatments-in-cucumis-seedlings
#1
Jie Deng, Hong-Jun Yu, Yun-Yun Li, Xiao-Meng Zhang, Peng Liu, Qiang Li, Wei-Jie Jiang
Nitrogen (N) is an important macronutrient for plant growth and development, but the regulatory mechanism of volatile compounds in response to N deficiency is not well understood, especially in cucumber, which consumes excessive N during growth. In this study, the major volatile compounds from cucumber leaves subjected to N deficiency were analyzed by GC-MS. A total of 24 volatile components were identified including 15 aldehydes, two ketones, two alkenes, and five other volatile compounds in 9930 leaves. Principal component analysis using volatile compounds from cucumber leaves provided good separation between N-sufficient and N-deficient treatments...
November 2, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27825413/risk-factors-of-lymphnode-metastasis-in-patients-with-thyroid-br-papillary-carcinoma-associated-with-graves-disease
#2
Yong Xie, Yue-Wu Liu, Meng-Yi Wang, Wen-Ze Wang, Hong-Feng Liu, Xiao-Yi Li, Wei-Sheng Gao
Objective To summarize the characteristics of lymph node metastasis in patients with papillary thyroid carcinoma accompanied with Graves disease,and to provide evidence for clinical treatment. Methods Totally 98 patients with papillary thyroid carcinoma and Graves disease who had been treated in Peking Union Medical College Hospital from January 2004 to December 2013 were divided into the lymph node metastasis positive group (n=34) and lymph node metastasis negative group (n=64). The general information,blood biochemical results,pathological results,and prognoses were compared between these two groups...
October 10, 2016: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/27764118/whole-genome-resequencing-of-a-cucumber-chromosome-segment-substitution-line-and-its-recurrent-parent-to-identify-candidate-genes-governing-powdery-mildew-resistance
#3
Qiang Xu, Yang Shi, Ting Yu, Xuewen Xu, Yali Yan, Xiaohua Qi, Xuehao Chen
Cucumber is an economically important vegetable crop worldwide. Powdery mildew (PM) is one of the most severe diseases that can affect cucumber crops. There have been several research efforts to isolate PM resistance genes for breeding PM-resistant cucumber. In the present study, we used a chromosome segment substitution line, SSL508-28, which carried PM resistance genes from the donor parent, JIN5-508, through twelve generations of backcrossing with a PM-susceptible inbred line, D8. We performed whole-genome resequencing of SSL508-28 and D8 to identify single nucleotide polymorphisms (SNPs), and insertions and deletions (indels)...
2016: PloS One
https://www.readbyqxmd.com/read/27545209/strain-differentiation-of-mycobacterium-tuberculosis-for-epidemiology-in-northwest-of-iran
#4
M Asgharzadeh, H Samadi Kafil, M Pourostadi, N Asadi Faezi, J Rashedi, B Mahdavipour
Tuberculosis is a major health problem throughout the world and there are still a great number of people with the disease. Planning for controlling tuberculosis is required to identify the sources of infection and screening for the disease. The aim of this study is the differentiation of Mycobacterium tuberculosis strains for better understands the spread of the disease in North West Iran. In this study, 194 positive cultures of M. tuberculosis in North West Iran were evaluated by the MIRU-VNTR method. MIRU-12 differentiated the 194 isolates into 138 different patterns, comprising 30 clusters and 108 unique patterns (HGDI=0...
2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27506367/development-and-optimization-of-ultra-high-performance-supercritical-fluid-chromatography-mass-spectrometry-method-for-high-throughput-determination-of-tocopherols-and-tocotrienols-in-human-serum
#5
Veronika Pilařová, Tomáš Gottvald, Pavel Svoboda, Ondřej Novák, Karolína Benešová, Sylvie Běláková, Lucie Nováková
The goal of this study was to develop an effective supercritical fluid chromatography method using single quadrupole MS for analysis of all isomeric forms of vitamin E. Finally, two fast and effective methods, the high resolution one and the high speed one, for the determination of 8 vitamin E isomers in human serum were developed. Rapid high-throughput liquid-liquid extraction was selected as a sample preparation step. Sample pretreatment of 100 μL human serum was consisted of protein precipitation with 200 μL ethanol and liquid-liquid extraction by 400 μL hexane/dichloromethane (80/20, v/v)...
August 31, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27233999/metabolic-syndrome-and-osteoporotic-fracture-a-population-based-study-in-china
#6
Li Qin, Zhen Yang, Weiwei Zhang, Hongxia Gu, Xiaoyong Li, Lingfei Zhu, Shuai Lu, Yin Xing, Hongmei Zhang, Yixin Niu, Guang Ning, Qing Su
BACKGROUND: Associations between metabolic syndrome (MetS) and osteoporotic fracture have been reported. However, the epidemiological studies are not conclusive. The objective of the study was to determine whether metabolic syndrome associates with osteoporotic fracture. METHODS: This was a cross-sectional study of 9930 Chinese adults aged 40 year or older in the Chongming District, Shanghai, China. A questionnaire, anthropometric measurements and laboratory tests were conducted...
2016: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/27150752/sonochemical-assisted-hydrothermal-synthesis-of-zno-cr-nanoparticles-loaded-activated-carbon-for-simultaneous-ultrasound-assisted-adsorption-of-ternary-toxic-organic-dye-derivative-spectrophotometric-optimization-kinetic-and-isotherm-study
#7
M Jamshidi, M Ghaedi, K Dashtian, S Hajati, A A Bazrafshan
Chromium doped zinc oxide nanoparticles (ZnO: Cr-NPs) was synthesized by ultrasonically assisted hydrothermal method and characterized by FE-SEM, XRD and TEM analysis. Subsequently, this composite ultrasonically assisted was deposited on activated carbon (ZnO: Cr-NPs-AC) and used for simultaneous ultrasound-assisted removal of three toxic organic dye namely of malachite green (MG), eosin yellow (EY) and Auramine O (AO). Dyes spectra overlap in mixture (major problem for simultaneous investigation) of this systems was extensively resolved by derivative spectrophotometric method...
September 2016: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/27147071/qtl-mapping-for-downy-mildew-resistance-in-cucumber-inbred-line-wi7120-pi-330628
#8
Yuhui Wang, Kyle VandenLangenberg, Todd C Wehner, Peter A G Kraan, Jos Suelmann, Xiangyang Zheng, Ken Owens, Yiqun Weng
Host resistance in WI7120 cucumber to prevailing downy mildew pathogen field populations is conferred by two major-effect, one moderate-effect and two minor-effect QTL. Downy mildew (DM) caused by the obligate oomycete Pseudoperonospora cubensis is the most devastating fungal disease of cucumber worldwide. The molecular mechanism of DM resistance in cucumber is poorly understood, and use of marker-assisted breeding for DM resistance is not widely available. Here, we reported QTL mapping results for DM resistance with 243 F2:3 families from the cross between DM-resistant inbred line WI7120 (PI 330628) and susceptible '9930'...
August 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27015676/a-fragment-substitution-in-the-promoter-of-cshdziv11-csgl3-is-responsible-for-fruit-spine-density-in-cucumber-cucumis-sativus-l
#9
Haiyang Zhang, Lina Wang, Shuangshuang Zheng, Zezhou Liu, Xiaoqin Wu, Zhihui Gao, Chenxing Cao, Qiang Li, Zhonghai Ren
The indel in the promoter of CsHDZIV11 co-segregates with fruit spine density and could be used for molecular breeding in cucumber. Fruit spine density is an important quality trait for marketing in cucumber (Cucumis sativus L.). However, the molecular basis of fruit spine density in cucumber remains unclear. In this study, we isolated a mutant, few spines 1 (fs1), from CNS2 (wild type, WT), a North China-type cucumber with a high density of fruit spines. Genetic analysis showed that fs1 was controlled by a single recessive Mendelian factor...
July 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/26878458/a-search-for-blues-brothers-x-ray-crystallographic-spectroscopic-characterization-of-the-tetraarylbenzidine-cation-radical-as-a-product-of-aging-of-solid-magic-blue
#10
Marat R Talipov, Mohammad M Hossain, Anitha Boddeda, Khushabu Thakur, Rajendra Rathore
Magic blue (MB+˙ SbCl6− salt), i.e. tris-4-bromophenylamminium cation radical, is a routinely employed one-electron oxidant that slowly decomposes in the solid state upon storage to form so called ‘blues brothers’, which often complicate the quantitative analyses of the oxidation processes. Herein, we disclose the identity of the main ‘blues brother’ as the cation radical and dication of tetrakis-(4-bromophenyl)benzidine (TAB) by a combined DFT and experimental approach, including isolation of TAB+˙ SbCl6− and its X-ray crystallography characterization...
March 14, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/26845560/a-new-glabrous-gene-csgl3-identified-in-trichome-development-in-cucumber-cucumis-sativus-l
#11
Jin-Ying Cui, Han Miao, Li-Hong Ding, Todd C Wehner, Pan-Na Liu, Ye Wang, Sheng-Ping Zhang, Xing-Fang Gu
Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore their use by growers can reduce pesticide residues. Previous studies have reported two glabrous mutant plants containing the genes, csgl1 and csgl2. In the present study, a new glabrous mutant, NCG157, was identified showing a gene interaction effect with csgl1 and csgl2. This mutant showed the glabrous character on stems, leaves, tendrils, receptacles and ovaries, and there were no spines or tumors on the fruit surface...
2016: PloS One
https://www.readbyqxmd.com/read/26653466/rapid-determination-of-88-veterinary-drug-residues-in-milk-using-automated-turborflow-online-clean-up-mode-coupled-to-liquid-chromatography-tandem-mass-spectrometry
#12
Wei-xia Zhu, Ji-zhou Yang, Zhao-xing Wang, Cai-juan Wang, Ya-feng Liu, Li Zhang
A novel method based on TurborFlow online solid phase extraction (SPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been established for simultaneous screening and confirmation of 88 wide-range veterinary drugs belonging to eight families (20 sulfonamides, 7 macrolides, 15 quinolones, 8 penicillins, 13 benzimidazoles, 4 tetracyclines, 2 sedatives, and 19 hormones) in milk. The preparation method consists of sample dilution and ultrasonic extraction, followed by an automated turbulent flow cyclone chromatography sample clean-up system...
February 1, 2016: Talanta
https://www.readbyqxmd.com/read/26631992/management-of-modifiable-risk-factors-prior-to-primary-hip-and-knee-arthroplasty-a-readmission-risk-assessment-tool
#13
Sreevathsa Boraiah, LiJin Joo, Ifeoma A Inneh, Parthiv Rathod, Morteza Meftah, Philip Band, Joseph A Bosco, Richard Iorio
BACKGROUND: Preoperative risk stratification and optimization of preoperative care may be helpful in reducing readmission rates after primary total joint arthroplasty. Assessment of the predictive value of individual modifiable risk factors without a tool to assess cumulative risk may not provide proper risk stratification of patients with regard to potential readmissions. As part of a Perioperative Orthopaedic Surgical Home model, we developed a scoring system, the Readmission Risk Assessment Tool (RRAT), which allows for risk stratification in patients undergoing elective primary total joint arthroplasty at our institution...
December 2, 2015: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/26444916/risk-factors-for-aspiration-pneumonia-in-older-adults
#14
Toshie Manabe, Shinji Teramoto, Nanako Tamiya, Jiro Okochi, Nobuyuki Hizawa
BACKGROUNDS: Aspiration pneumonia is a dominant form of community-acquired and healthcare-associated pneumonia, and a leading cause of death among ageing populations. However, the risk factors for developing aspiration pneumonia in older adults have not been fully evaluated. The purpose of the present study was to determine the risk factors for aspiration pneumonia among the elderly. METHODOLOGY AND PRINCIPAL FINDINGS: We conducted an observational study using data from a nationwide survey of geriatric medical and nursing center in Japan...
2015: PloS One
https://www.readbyqxmd.com/read/26350497/a-novel-allele-of-monoecious-m-locus-is-responsible-for-elongated-fruit-shape-and-perfect-flowers-in-cucumber-cucumis-sativus-l
#15
Junyi Tan, Qianyi Tao, Huanhuan Niu, Zhen Zhang, Dandan Li, Zhenhui Gong, Yiqun Weng, Zheng Li
A 14 bp deletion in CsACS2 gene encoding a truncated loss-of-function protein is responsible for elongated fruit shape and perfect flowers in cucumber. In cucumber (Cucumis sativus L.), sex expression and fruit shape are important components of biological and marketable yield. The association of fruit shape and sex expression is a very interesting phenomenon. The sex determination is controlled primarily by the F (female) and M (monoecy) loci. Homozygous recessive mm plants bear bisexual (perfect) flowers, and the fruits are often round shaped...
December 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/26239410/fine-genetic-mapping-of-the-white-immature-fruit-color-gene-w-to-a-33-0-kb-region-in-cucumber-cucumis-sativus-l
#16
Hanqiang Liu, Huanwen Meng, Yupeng Pan, Xinjing Liang, Jianqing Jiao, Yuhong Li, Shuxia Chen, Zhihui Cheng
The white immature fruit color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin color of immature fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature fruit color genes in cucumbers...
December 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/26048092/qtl-mapping-in-multiple-populations-and-development-stages-reveals-dynamic-quantitative-trait-loci-for-fruit-size-in-cucumbers-of-different-market-classes
#17
Yiqun Weng, Marivi Colle, Yuhui Wang, Luming Yang, Mor Rubinstein, Amir Sherman, Ron Ophir, Rebecca Grumet
QTL analysis in multi-development stages with different QTL models identified 12 consensus QTLs underlying fruit elongation and radial growth presenting a dynamic view of genetic control of cucumber fruit development. Fruit size is an important quality trait in cucumber (Cucumis sativus L.) of different market classes. However, the genetic and molecular basis of fruit size variations in cucumber is not well understood. In this study, we conducted QTL mapping of fruit size in cucumber using F2, F2-derived F3 families and recombinant inbred lines (RILs) from a cross between two inbred lines Gy14 (North American picking cucumber) and 9930 (North China fresh market cucumber)...
September 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/25928321/the-photoinduced-e-%C3%A2-z-isomerization-of-bisazobenzenes-a-surface-hopping-molecular-dynamics-study
#18
Gereon Floss, Peter Saalfrank
The photoinduced E → Z isomerization of azobenzene is a prototypical example of molecular switching. On the way toward rigid molecular rods such as those for opto-mechanical applications, multiazobenzene structures have been suggested in which several switching units are linked together within the same molecule (Bléger et al., J. Phys. Chem. B 2011, 115, 9930-9940). Large differences in the switching efficiency of multiazobenzenes have been observed, depending on whether the switching units are electronically decoupled or not...
May 21, 2015: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/25881429/-determination-of-carmine-in-carbonated-beverages-using-3-d-fluorescence-spectra-coupled-with-second-order-calibration-algorithm
#19
Run Li, Guo-qing Chen, Chun Zhu, Fan-biao Kong, Yang-jun Hu
Three-dimensional fluorescence spectra combined with second-order calibration algorithm based on alternate a weighted residual (ANWE) was applied to the direct concentration determination of carmine in carbonated beverages. Firstly, 3-D fluorescence spectra of carmine and sunset yellow mixed solutions with different concentrations were obtained by employing spectrometer, and analyzed by using ANWE, the correlation coefficient between calibration concentration and the actual concentration was 0.9917, and the average recovery was 100...
December 2014: Guang Pu Xue Yu Guang Pu Fen Xi, Guang Pu
https://www.readbyqxmd.com/read/25874931/ultrahigh-density-linkage-map-for-cultivated-cucumber-cucumis-sativus-l-using-a-single-nucleotide-polymorphism-genotyping-array
#20
Mor Rubinstein, Mark Katzenellenbogen, Ravit Eshed, Ada Rozen, Nurit Katzir, Marivi Colle, Luming Yang, Rebecca Grumet, Yiqun Weng, Amir Sherman, Ron Ophir
Genotyping arrays are tools for high-throughput genotyping, which is beneficial in constructing saturated genetic maps and therefore high-resolution mapping of complex traits. Since the report of the first cucumber genome draft, genetic maps have been constructed mainly based on simple-sequence repeats (SSRs) or on combinations of SSRs and sequence-related amplified polymorphism (SRAP). In this study, we developed the first cucumber genotyping array consisting of 32,864 single-nucleotide polymorphisms (SNPs)...
2015: PloS One
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