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https://www.readbyqxmd.com/read/28103327/isolation-characterization-and-evaluation-of-collagen-from-jellyfish-rhopilema-esculentum-kishinouye-for-use-in-hemostatic-applications
#1
Xiaochen Cheng, Ziyu Shao, Chengbo Li, Lejun Yu, Mazhar Ali Raja, Chenguang Liu
Hemostat has been a crucial focus since human body is unable to control massive blood loss, and collagen proves to be an effective hemostat in previous studies. In this study, collagen was isolated from the mesoglea of jellyfish Rhopilema esculentum Kishinouye and its hemostatic property was studied. The yields of acid-soluble collagen (ASC) and pepsin-soluble (PSC) were 0.12% and 0.28% respectively. The SDS-PAGE patterns indicated that the collagen extracted from jellyfish mesoglea was type I collagen. The lyophilized jellyfish collagen sponges were cross-linked with EDC and interconnected networks in the sponges were revealed by scanning electron microscope (SEM)...
2017: PloS One
https://www.readbyqxmd.com/read/28038748/structural-characterization-and-immunomodulatory-activity-of-a-new-polysaccharide-from-jellyfish
#2
Qiang-Ming Li, Jing-Fei Wang, Xue-Qiang Zha, Li-Hua Pan, Hai-Lin Zhang, Jian-Ping Luo
A new polysaccharide (JSP-11) with a molecular weight of 1.25×10(6)Da was extracted and purified from jellyfish. Monosaccharide analysis showed that JSP-11 was composed of mannose, galactose and glucuronic acid with a molar ratio of 2.18:1.00:1.94. According to the analysis of fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, JSP-11 was determined to contain a linear backbone which consisted of (1→3,6)-linked β-d-Manp and (1→6)-linked β-d-Galp. The branch of (1→)-linked α-d-GlcpA was attached to the C-3 position of (1→3,6)-linked β-d-Manp in the backbone...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28000727/evidence-of-cnidarians-sensitivity-to-sound-after-exposure-to-low-frequency-noise-underwater-sources
#3
Marta Solé, Marc Lenoir, José Manuel Fontuño, Mercè Durfort, Mike van der Schaar, Michel André
Jellyfishes represent a group of species that play an important role in oceans, particularly as a food source for different taxa and as a predator of fish larvae and planktonic prey. The massive introduction of artificial sound sources in the oceans has become a concern to science and society. While we are only beginning to understand that non-hearing specialists like cephalopods can be affected by anthropogenic noises and regulation is underway to measure European water noise levels, we still don't know yet if the impact of sound may be extended to other lower level taxa of the food web...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27964856/ingestion-of-microplastics-by-natural-zooplankton-groups-in-the-northern-south-china-sea
#4
Xiaoxia Sun, Qingjie Li, Mingliang Zhu, Junhua Liang, Shan Zheng, Yongfang Zhao
The ingestion of microplastics by five natural zooplankton groups in the northern South China Sea was studied for the first time and two types of sampling nets (505μm and 160μm in mesh size) were compared. The microplastics were detected in zooplankton sampled from 16 stations, with the fibrous microplastics accounting for the largest proportion (70%). The main component of the found microplastics was polyester. The average length of the microplastics was 125μm and 167μm for Nets I and II, respectively...
December 10, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27942028/recovery-of-coastal-fauna-after-the-2011-tsunami-in-japan-as-determined-by-bimonthly-underwater-visual-censuses-conducted-over-five-years
#5
Reiji Masuda, Makoto Hatakeyama, Katsuhide Yokoyama, Masaru Tanaka
Massive tsunamis induce catastrophic disturbance in marine ecosystems, yet they can provide unique opportunities to observe the process of regeneration. Here, we report the recovery of fauna after the 2011 tsunami in northeast Japan based on underwater visual censuses performed every two months over five years. Both total fish abundance and species richness increased from the first to the second year after the tsunami followed by stabilization in the following years. Short-lived fish, such as the banded goby Pterogobius elapoides, were relatively abundant in the first two years, whereas long-lived species, such as the black rockfish Sebastes cheni, increased in the latter half of the survey period...
2016: PloS One
https://www.readbyqxmd.com/read/27925224/fluorescent-protein-photoprotein-fusions-and-their-applications-in-calcium-imaging
#6
Adil Bakayan, Beatriz Domingo, Cecilia F Vaquero, Nadine Peyriéras, Juan Llopis
Calcium-activated photoproteins, such as aequorin, have been used as luminescent Ca(2+) indicators since 1967. After the cloning of aequorin in 1985, microinjection was substituted by its heterologous expression, which opened the way for a widespread use. Molecular fusion of GFP to aequorin recapitulated the nonradiative energy transfer process that occurs in the jellyfish Aequorea victoria, from which these two proteins were obtained, resulting in an increase of light emission and a shift to longer wavelength...
December 7, 2016: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/27914077/making-fluorescent-streptococci-and-enterococci-for-live-imaging
#7
Sarah Shabayek, Barbara Spellerberg
Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, outstanding fluorescent labeling tools with numerous applications in vastly different areas of life sciences have been developed. To optimize GFP for diverse life science applications, a large variety of GFP derivatives with different environmental characteristics have been generated by mutagenesis. The enhanced green fluorescent protein (EGFP) is a well-known GFP derivative with highly increased fluorescence intensity compared to the GFP wild-type molecule...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27894263/erratum-to-a-new-transcriptome-and-transcriptome-profiling-of-adult-and-larval-tissue-in-the-box-jellyfish-alatina-alata-an-emerging-model-for-studying-venom-vision-and-sex
#8
Cheryl Lewis Ames, Joseph F Ryan, Alexandra E Bely, Paulyn Cartwright, Allen G Collins
No abstract text is available yet for this article.
November 28, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27890264/selective-suppression-of-in-situ-proliferation-of-scyphozoan-polyps-by-biofouling
#9
Song Feng, Shi-Wei Wang, Guang-Tao Zhang, Song Sun, Fang Zhang
An increase in marine artificial constructions has been proposed as a major cause of jellyfish blooms, because these constructions provide additional substrates for organisms at the benthic stage (polyps), which proliferate asexually and release a large amount of free-swimming medusae. These hard surfaces are normally covered by fouling communities, the components of which have the potential to impede the proliferation of polyps. In this study, we report an in situ experiment of polyp survival of four large scyphozoan species found in East Asian marginal seas that were exposed to biofouling, a universal phenomenon occurring on marine artificial constructions...
November 24, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27881808/jellyfish-like-accessory-mitral-valve-tissue-causing-near-collapse-in-a-young-child
#10
Hideyuki Kato, Carolina Escudero, Elizabeth Sherwin, Martin Hosking, Sanjiv K Gandhi
Accessory mitral valve tissue (AMVT) causing left ventricular outflow tract obstruction (LVOTO) is rare. We report a case of AMVT causing severe LVOTO resulting in acutely progressive symptoms of near-collapse. Urgent surgical resection eliminated the patient's life-threatening symptoms. AMVT should be considered among potential LVOTO diagnoses, and early surgical intervention may be required.
November 23, 2016: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/27880678/evidence-for-an-alternative-mechanism-of-toxin-production-in-the-box-jellyfish-alatina-alata
#11
Cheryl Lewis Ames, Jason Macrander
Cubozoans (box jellyfish) have a reputation as the most venomous animals on the planet. Herein, we provide a review of cubozoan prey capture and digestion informed by the scientific literature. Like all cnidarians, box jellyfish envenomation originates from structures secreted within nematocyte post-Golgi vesicles called nematocysts. When tentacles come in contact with prey or would-be predators, a cocktail of toxins is rapidly deployed from nematocysts via a long spiny tubule that serves to immobilize the target organism...
November 2016: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/27878085/stable-isotope-based-trophic-structure-of-pelagic-fish-and-jellyfish-across-natural-and-anthropogenic-landscape-gradients-in-a-fjord-estuary
#12
Sean M Naman, Correigh M Greene, Casimir A Rice, Joshua Chamberlin, Letitia Conway-Cranos, Jeffery R Cordell, Jason E Hall, Linda D Rhodes
Identifying causes of structural ecosystem shifts often requires understanding trophic structure, an important determinant of energy flow in ecological communities. In coastal pelagic ecosystems worldwide, increasing jellyfish (Cnidaria and Ctenophora) at the expense of small fish has been linked to anthropogenic alteration of basal trophic pathways. However, this hypothesis remains untested in part because baseline description of fish-jellyfish trophic dynamics, and the environmental features that influence them are lacking...
November 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/27872215/intramolecular-interactions-that-control-voltage-sensitivity-in-the-jshak1-potassium-channel-from-polyorchis-penicillatus
#13
Nazlee Sharmin, Warren J Gallin
Voltage-gated potassium ion (Kv) channel proteins respond to changes in membrane potential by changing the probability of K(+) flux through an ion-selective pore. Kv channels from different paralogous and orthologous families have widely varying V50 values. The voltage-sensing transmembrane helices (S4) of different channels contain 4-7 basic residues that are responsible for transducing changes in transmembrane potential into the energy required to shift the equilibrium between the open- and closed-channel conformations...
November 21, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27852126/traditional-chinese-medicine-food-therapy-and-hypertension-control-a-narrative-review-of-chinese-literature
#14
Ping Zou
Despite the lack of English literature about Traditional Chinese Medicine (TCM) food therapy, there is abundant Chinese literature about the application of food therapy for hypertension control. This paper summarizes basic concepts of TCM, the principles of food therapy and its application for hypertension control according to Chinese literature. In TCM, food is conceptualized according to both nutritional and functional aspects, and can be used to treat illnesses. Four principles of TCM food therapy including light eating, balancing the "hot" and "cold" nature of food, the harmony of the five flavors of food, and consistency between dietary intake and different health conditions, can be used to facilitate hypertension control...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27845203/complete-mitochondrial-genome-and-evolutionary-analysis-of-turritopsis-dohrnii-the-immortal-jellyfish-with-a-reversible-life-cycle
#15
A A Lisenkova, A P Grigorenko, T V Tyazhelova, T V Andreeva, F E Gusev, A D Manakhov, A Yu Goltsov, S Piraino, M P Miglietta, E I Rogaev
Turritopsis dohrnii (Cnidaria, Hydrozoa, Hydroidolina, Anthoathecata) is the only known metazoan that is capable of reversing its life cycle via morph rejuvenation from the adult medusa stage to the juvenile polyp stage. Here, we present a complete mitochondrial (mt) genome sequence of T. dohrnii, which harbors genes for 13 proteins, two transfer RNAs, and two ribosomal RNAs. The T. dohrnii mt genome is characterized by typical features of species in the Hydroidolina subclass, such as a high A+T content (71...
November 11, 2016: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/27826020/biochemical-and-kinetic-evaluation-of-the-enzymatic-toxins-from-two-stinging-scyphozoans-nemopilema-nomurai-and-cyanea-nozakii
#16
Yang Yue, Huahua Yu, Rongfeng Li, Ronge Xing, Song Liu, Kecheng Li, Xueqin Wang, Xiaolin Chen, Pengcheng Li
Jellyfish envenomations are emerging as an important public health concern occurred worldwide. In China, the situation is getting worse with numerous people stung by jellyfish Nemopilema nomurai (N. nomurai) and Cyanea nozakii (C. nozakii) in the summer. However, the proteinaceous mixtures in nematocysts responsible for the symptoms of jellyfish stings were scarcely characterized and understood in view of enzymatic constituents and toxicity. In the present study, enzymatic properties of jellyfish N. nomurai and C...
January 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27820907/box-jellyfish-alatina-alata-has-a-circumtropical-distribution
#17
Jonathan W Lawley, Cheryl Lewis Ames, Bastian Bentlage, Angel Yanagihara, Roger Goodwill, Ehsan Kayal, Kikiana Hurwitz, Allen G Collins
Species of the box jellyfish (Cubozoa) genus Alatina are notorious for their sting along the beaches of several localities of the Atlantic and Pacific. These species include Alatina alata on the Caribbean Island of Bonaire (the Netherlands), A. moseri in Hawaii, and A. mordens in Australia. Most cubozoans inhabit coastal waters, but Alatina is unusual in that specimens have also been collected in the open ocean at great depths. Alatina is notable in that populations form monthly aggregations for spermcast mating in conjunction with the lunar cycle...
October 2016: Biological Bulletin
https://www.readbyqxmd.com/read/27816720/multivacuolated-mucin-filled-cells-a-unique-cell-characteristic-of-plexiform-neurofibroma-a-report-of-11-cases
#18
Michael Michal, Dmitry V Kazakov, Ladislav Hadravský, Květoslava Michalová, Boris Rychlý, Michal Michal
The authors present 11 cases of plexiform neurofibroma (PN) that featured a very characteristic type of cell appearing as multivacuolated mucin-filled cells (MMFC). The 11 cases were obtained after reviewing 109 cases of PN. Six out of 10 patients showed clinical features of neurofibromatosis type 1 (NF1). The size of PN ranged from 0.8 cm to 11.5 cm in the largest dimension. The lesions represented classical PN in all cases with myxoid, hypocellular stroma. The MMFC were found within the most myxoid tumorous nodules and were haphazardly located, typically featuring a variably sized, multivacuolated cytoplasm divided by a fine septa with a small polygonal nucleus on one side, which was often compressed or slightly indented by the cytoplasmic mucous substances...
November 2, 2016: Human Pathology
https://www.readbyqxmd.com/read/27815048/electrophysiological-activity-of-a-neurotoxic-fraction-from-the-venom-of-box-jellyfish-carybdea-marsupialis
#19
Fernando Lazcano-Pérez, Rogelio O Arellano, Edith Garay, Roberto Arreguín-Espinosa, Judith Sánchez-Rodríguez
Carybdea marsupialis is a widely distributed box jellyfish found in the Mediterranean and in the tropical waters of the Caribbean Sea. Its venom is a complex mixture of biologically active compounds that are used to catch prey. In order to evaluate the activity of the neurotoxins in the venom, bioassays were carried out using the marine crab Ocypode quadrata. The proteins with neurotoxic effect were partially purified using low-pressure liquid chromatography techniques. Gel filtration (Sephadex G-50M) was used as the first step and the active fraction in crabs was passed through a QAE Sephadex A-25 column...
January 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/27812408/comparative-internal-anatomy-of-staurozoa-cnidaria-with-functional-and-evolutionary-inferences
#20
Lucília S Miranda, Allen G Collins, Yayoi M Hirano, Claudia E Mills, Antonio C Marques
Comparative efforts to understand the body plan evolution of stalked jellyfishes are scarce. Most characters, and particularly internal anatomy, have neither been explored for the class Staurozoa, nor broadly applied in its taxonomy and classification. Recently, a molecular phylogenetic hypothesis was derived for Staurozoa, allowing for the first broad histological comparative study of staurozoan taxa. This study uses comparative histology to describe the body plans of nine staurozoan species, inferring functional and evolutionary aspects of internal morphology based on the current phylogeny of Staurozoa...
2016: PeerJ
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