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https://www.readbyqxmd.com/read/28334693/fabrication-of-human-hair-keratin-jellyfish-collagen-eggshell-derived-hydroxyapatite-osteoinductive-biocomposite-scaffolds-for-bone-tissue-engineering-from-waste-to-regenerative-medicine-products
#1
Yavuz Emre Arslan, Tugba Sezgin Arslan, Burak Derkus, Emel Emregul, Kaan C Emregul
In the present study, we aimed at fabricating an osteoinductive biocomposite scaffold using keratin obtained from human hair, jellyfish collagen and eggshell-derived nano-sized spherical hydroxyapatite (nHA) for bone tissue engineering applications. Keratin, collagen and nHA were characterized with the modified Lowry method, free-sulfhydryl groups and hydroxyproline content analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) and thermal gravimetric analysis (TGA) which confirmed the success of the extraction and/or isolation processes...
March 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28331896/hematological-parameters-on-the-effect-of-the-jellyfish-venom-cassiopea-andromeda-in-animal-models
#2
Iraj Nabipour, Gholamhossein Mohebbi, Hossein Vatanpour, Amir Vazirizadeh
For the first time, we previously recorded an enormous population of the Cassiopea andromeda jellyfish that had increased dramatically from Bushehr coasts of Iran. The sub-acute toxicity of the jellyfish venom in rat organs was correspondingly carried out. The data presented in this paper relate to the in vivo and in vitro hematological effects of this venomous species of jellyfish venom.
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28265497/indomethacin-reproducibly-induces-metamorphosis-in-cassiopea-xamachana-scyphistomae
#3
Patricia Cabrales-Arellano, Tania Islas-Flores, Patricia E Thomé, Marco A Villanueva
Cassiopea xamachana jellyfish are an attractive model system to study metamorphosis and/or cnidarian-dinoflagellate symbiosis due to the ease of cultivation of their planula larvae and scyphistomae through their asexual cycle, in which the latter can bud new larvae and continue the cycle without differentiation into ephyrae. Then, a subsequent induction of metamorphosis and full differentiation into ephyrae is believed to occur when the symbionts are acquired by the scyphistomae. Although strobilation induction and differentiation into ephyrae can be accomplished in various ways, a controlled, reproducible metamorphosis induction has not been reported...
2017: PeerJ
https://www.readbyqxmd.com/read/28258523/analysis-of-pelagia-noctiluca-proteome-reveals-a-red-fluorescent-protein-a-zinc-metalloproteinase-and-a-peroxiredoxin
#4
Bárbara Frazão, Alexandre Campos, Hugo Osório, Benjamin Thomas, Sérgio Leandro, Alexandre Teixeira, Vitor Vasconcelos, Agostinho Antunes
Pelagia noctiluca is the most venomous jellyfish in the Mediterranean Sea where it forms dense blooms. Although there is several published research on this species, until now none of the works has been focused on a complete protein profile of the all body constituents of this organism. Here, we have performed a detailed proteomics characterization of the major protein components expressed by P. noctiluca. With that aim, we have considered the study of jellyfish proteins involved in defense, body constituents and metabolism, and furthered explore the significance and potential application of such bioactive molecules...
March 3, 2017: Protein Journal
https://www.readbyqxmd.com/read/28250103/the-nematocyst-s-sting-is-driven-by-the-tubule-moving-front
#5
Sinwook Park, Gadi Piriatinskiy, Dan Zeevi, Jonathan Ben-David, Gilad Yossifon, Uri Shavit, Tamar Lotan
The nematocyst is the explosive injection system of the phylum Cnidaria, and is one of the fastest delivery systems found in Nature. Exploring its injection mechanism is key for understanding predator-prey interactions and protection against jellyfish stinging. Here we analyse the injection of jellyfish nematocysts and ask how the build-up of the poly-γ-glutamate (pγGlu) osmotic potential inside the nematocyst drives its discharge. To control the osmotic potential, we used a two-channel microfluidic system to direct the elongating nematocyst tubule through oil, where no osmotic potential can develop, while keeping the nematocyst capsule in water at all times...
March 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28245277/environmental-dna-reflects-spatial-and-temporal-jellyfish-distribution
#6
Toshifumi Minamoto, Miho Fukuda, Koki R Katsuhara, Ayaka Fujiwara, Shunsuke Hidaka, Satoshi Yamamoto, Kohji Takahashi, Reiji Masuda
Recent development of environmental DNA (eDNA) analysis allows us to survey underwater macro-organisms easily and cost effectively; however, there have been no reports on eDNA detection or quantification for jellyfish. Here we present the first report on an eDNA analysis of marine jellyfish using Japanese sea nettle (Chrysaora pacifica) as a model species by combining a tank experiment with spatial and temporal distribution surveys. We performed a tank experiment monitoring eDNA concentrations over a range of time intervals after the introduction of jellyfish, and quantified the eDNA concentrations by quantitative real-time PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28240847/persian-gulf-jellyfish-cassiopea-andromeda-venom-fractions-induce-selective-injury-and-cytochrome-c-release-in-mitochondria-obtained-from-breast-adenocarcinoma-patients
#7
Mohammad Reza Mirshamsi, Ramesh Omranipour, Amir Vazirizadeh, Amir Fakhri, Fatemeh Zangeneh, Gholam Hussain Mohebbi, Ramin Seyedian, Jalal Pourahmad
Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed...
January 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28235909/crying-a-river-how-much-salt-laden-jelly-can-a-leatherback-turtle-really-eat
#8
John Davenport
Leatherback turtles (Dermochelys coriacea) are capital breeders that accumulate blubber (33 kJ g wet mass(-1)) by hyperphagia on a gelatinous diet at high latitudes; they breed in the tropics. A jellyfish diet is energy-poor (0.1-0.2 kJ g wet mass(-1)), so leatherbacks must ingest large quantities. Two published estimates of feeding rate (50% body mass d(-1) (on Rhizostoma pulmo), 73% body mass d(-1) (on Cyanea capillata)) have been criticised as too high. Jellyfish have high salt and water contents that must be removed to access organic material and energy...
February 24, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28216728/marine-and-other-aquatic-dermatoses
#9
Surg Capt Jandhyala Sridhar, Surg Cdr Rajeev Deo
Occupational and recreational aquatic activity predisposes our population to a wide variety of dermatoses. Sunburn, urticaria, jellyfish stings, and contact dermatitis to rubber equipment are common allergies that are encountered in the aquatic environment. Among the infections, tinea versicolor, intertrigo, and verruca vulgaris are widespread. Swimmer's itch may occur due to skin penetration by schistosome cercariae, while free-floating nematocysts of marine coelenterates may precipitate seabather's eruption...
January 2017: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/28212329/enzyme-assisted-discovery-of-antioxidant-peptides-from-edible-marine-invertebrates-a-review
#10
REVIEW
Tsun-Thai Chai, Yew-Chye Law, Fai-Chu Wong, Se-Kwon Kim
Marine invertebrates, such as oysters, mussels, clams, scallop, jellyfishes, squids, prawns, sea cucumbers and sea squirts, are consumed as foods. These edible marine invertebrates are sources of potent bioactive peptides. The last two decades have seen a surge of interest in the discovery of antioxidant peptides from edible marine invertebrates. Enzymatic hydrolysis is an efficient strategy commonly used for releasing antioxidant peptides from food proteins. A growing number of antioxidant peptide sequences have been identified from the enzymatic hydrolysates of edible marine invertebrates...
February 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28196712/ipomoea-pes-caprae-l-r-br-convolvulaceae-as-a-topical-herbal-medicine-to-relieve-pain-and-inflammation-promising-tool-against-effects-due-to-toxin-skin-contact
#11
Cristiane da Silva Barth, Hugo Guilherme Tolentino de Souza, Lilian W Rocha, Gislaine Francieli da Silva, Mariana Ferreira Dos Anjos, Veronica D'Avila Pastor, Tania Mari Belle Bresolin, Angelica Garcia Couto, José Roberto Santin, Nara Lins Meira Quintão
ETHNOPHARMACOLOGICAL RELEVANCE: Ipomoea pes-caprae is known as bayhops, beach morning glory or goat's foot, and in Brazil as salsa-de-praia. Its leaves are used in worldwide folk medicine for the relief of jellyfish-stings symptoms. The literature only reports the anti-inflammatory and neutralizing effects of a nonpolar plant derived, without validating the popular use or exploring the mechanism of action. AIM OF THE STUDY: This study aimed to evaluate and validate the topical effects of a semisolid containing hydroethanolic extract obtained from the aerial parts of I...
February 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28190838/determination-of-aluminum-in-edible-jellyfish-using-chrome-azurol-s-with-spot-test-on-filter-paper
#12
Dai Cheng, Xinyu Zhang, Xiang Li, Lihua Hou, Chunling Wang
Aluminum (Al) has been well known as an environmental factor that may affect several enzymes and other biomolecules related to Alzheimer's disease. The increasing use of Al in the preparation and storage of food currently represents the main form of Al exposure for the general public. The present study was aimed to develop a household procedure for the rapid test determination of Al in edible jellyfish. The method was developed based on the reaction of Chrome Azurol S with Al in acidic medium, forming a colored compound on the surface of filter paper...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28187575/stalked-jellyfishes-cnidaria-staurozoa-of-south-africa-with-the-description-of-i-calvadosia-i-i-lewisi-i-sp-nov
#13
Lucília S Miranda, George M Branch, Allen G Collins, Yayoi M Hirano, Antonio C Marques, Charles L Griffiths
Stalked jellyfishes (Cnidaria: Staurozoa) are cryptic, benthic animals, known mainly from polar and temperate waters of the Northern Hemisphere. We describe a new species, Calvadosia lewisi, from South Africa and review the staurozoan fauna of the region. Three other species are previously known from South Africa: Calvadosia capensis (Carlgren, 1938); Depastromorpha africana Carlgren, 1935; and Lipkea stephensoni Carlgren, 1933, but all of these are known from very few records and have been poorly illustrated and documented to date...
February 3, 2017: Zootaxa
https://www.readbyqxmd.com/read/28185799/exploring-single-nucleotide-polymorphism-snp-microsatellite-ssr-and-differentially-expressed-genes-in-the-jellyfish-rhopilema-esculentum-by-transcriptome-sequencing
#14
Yunfeng Li, Zunchun Zhou, Meilin Tian, Yi Tian, Ying Dong, Shilei Li, Weidong Liu, Chongbo He
In this study, single nucleotide polymorphism (SNP), microsatellite (SSR) and differentially expressed genes (DEGs) in the oral parts, gonads, and umbrella parts of the jellyfish Rhopilema esculentum were analyzed by RNA-Seq technology. A total of 76.4 million raw reads and 72.1 million clean reads were generated from deep sequencing. Approximately 119,874 tentative unigenes and 149,239 transcripts were obtained. A total of 1,034,708 SNP markers were detected in the three tissues. For microsatellite mining, 5088 SSRs were identified from the unigene sequences...
February 6, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28159636/computational-comparison-of-a-calcium-dependent-jellyfish-protein-apoaequorin-and-calmodulin-cholesterol-in-short-term-memory-maintenance
#15
Gene A Morrill, Adele B Kostellow, Raj K Gupta
Memory reconsolidation and maintenance depend on calcium channels and on calcium/calmodulin-dependent kinases regulating protein turnover in the hippocampus. Ingestion of a jellyfish protein, apoaequorin, reportedly protects and/or improves verbal learning in adults and is currently widely advertised for use by the elderly. Apoaequorin is a member of the EF-hand calcium binding family of proteins that includes calmodulin. Calmodulin-1 (148 residues) differs from Apoaequorin (195 residues) in that it contains four rather than three Ca(2+)-binding sites and three rather than four cholesterol-binding (CRAC, CARC) domains...
March 6, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28134758/functional-elucidation-of-nemopilema-nomurai-and-cyanea-nozakii-nematocyst-venoms-lytic-activity-using-mass-spectrometry-and-zymography
#16
Yang Yue, Huahua Yu, Rongfeng Li, Ronge Xing, Song Liu, Kecheng Li, Xueqin Wang, Xiaolin Chen, Pengcheng Li
BACKGROUND: Medusozoans utilize explosively discharging penetrant nematocysts to inject venom into prey. These venoms are composed of highly complex proteins and peptides with extensive bioactivities, as observed in vitro. Diverse enzymatic toxins have been putatively identified in the venom of jellyfish, Nemopilema nomurai and Cyanea nozakii, through examination of their proteomes and transcriptomes. However, functional examination of putative enzymatic components identified in proteomic approaches to elucidate potential bioactivities is critically needed...
January 26, 2017: Toxins
https://www.readbyqxmd.com/read/28133573/jellyfish-extract-induces-apoptotic-cell-death-through-the-p38-pathway-and-cell-cycle-arrest-in-chronic-myelogenous-leukemia-k562-cells
#17
Sun-Hyung Ha, Fansi Jin, Choong-Hwan Kwak, Fukushi Abekura, Jun-Young Park, Nam Gyu Park, Young-Chae Chang, Young-Choon Lee, Tae-Wook Chung, Ki-Tae Ha, Jong-Keun Son, Hyeun Wook Chang, Cheorl-Ho Kim
Jellyfish species are widely distributed in the world's oceans, and their population is rapidly increasing. Jellyfish extracts have several biological functions, such as cytotoxic, anti-microbial, and antioxidant activities in cells and organisms. However, the anti-cancer effect of Jellyfish extract has not yet been examined. We used chronic myelogenous leukemia K562 cells to evaluate the mechanisms of anti-cancer activity of hexane extracts from Nomura's jellyfish in vitro. In this study, jellyfish are subjected to hexane extraction, and the extract is shown to have an anticancer effect on chronic myelogenous leukemia K562 cells...
2017: PeerJ
https://www.readbyqxmd.com/read/28120793/the-global-susceptibility-of-coastal-forage-fish-to-competition-by-large-jellyfish
#18
Nicolas Azaña Schnedler-Meyer, Patrizio Mariani, Thomas Kiørboe
Competition between large jellyfish and forage fish for zooplankton prey is both a possible cause of jellyfish increases and a concern for the management of marine ecosystems and fisheries. Identifying principal factors affecting this competition is therefore important for marine management, but the lack of both good quality data and a robust theoretical framework have prevented general global analyses. Here, we present a general mechanistic food web model that considers fundamental differences in feeding modes and predation pressure between fish and jellyfish...
November 16, 2016: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28112211/crude-venom-from-nematocysts-of-pelagia-noctiluca-cnidaria-scyphozoa-elicits-a-sodium-conductance-in-the-plasma-membrane-of-mammalian-cells
#19
Rossana Morabito, Roberta Costa, Valentina Rizzo, Alessia Remigante, Charity Nofziger, Giuseppa La Spada, Angela Marino, Markus Paulmichl, Silvia Dossena
Cnidarians may negatively impact human activities and public health but concomitantly their venom represents a rich source of bioactive substances. Pelagia noctiluca is the most venomous and abundant jellyfish of the Mediterranean Sea and possesses a venom with hemolytic and cytolytic activity for which the mechanism is largely unknown. Here we show that exposure of mammalian cells to crude venom from the nematocysts of P. noctiluca profoundly alters the ion conductance of the plasma membrane, therefore affecting homeostatic functions such as the regulation and maintenance of cellular volume...
January 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28103327/isolation-characterization-and-evaluation-of-collagen-from-jellyfish-rhopilema-esculentum-kishinouye-for-use-in-hemostatic-applications
#20
Xiaochen Cheng, Ziyu Shao, Chengbo Li, Lejun Yu, Mazhar Ali Raja, Chenguang Liu
Hemostat has been a crucial focus since human body is unable to control massive blood loss, and collagen proves to be an effective hemostat in previous studies. In this study, collagen was isolated from the mesoglea of jellyfish Rhopilema esculentum Kishinouye and its hemostatic property was studied. The yields of acid-soluble collagen (ASC) and pepsin-soluble (PSC) were 0.12% and 0.28% respectively. The SDS-PAGE patterns indicated that the collagen extracted from jellyfish mesoglea was type I collagen. The lyophilized jellyfish collagen sponges were cross-linked with EDC and interconnected networks in the sponges were revealed by scanning electron microscope (SEM)...
2017: PloS One
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