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https://www.readbyqxmd.com/read/27911492/rolling-circle-amplification-with-chemically-modified-nucleoside-triphosphates
#1
Marcel Hollenstein, Masad J Damha
Modified nucleoside triphosphates (dN*TPs) represent facile and versatile precursors for the introduction of chemical diversity into nucleic acids. While dN*TPs have been utilized in a plethora of practical applications, very little attention has been devoted to the assessment of their compatibility with isothermal amplification strategies. In this context, rolling circle amplification (RCA) is a wide-spread enzymatic replication method in which small single-stranded DNA (ssDNA) circles serve as templates in primer extension reactions yielding very long, ssDNA products...
December 1, 2016: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/27911473/enhancement-of-upconverted-fluorescence-by-interference-layers
#2
Janina Wirth, Kory K Green, Megan O'Connor, Shuang F Lim
Upconverting nanoparticles show potential applications in the field of photovoltaics and array-based detection devices. While fluorescence enhancement using interference of incident radiation is well known in Stokes-shift type systems such as fluorescent dyes; the effect of such interference geometry in nonlinear Anti-Stokes type emission, such as in upconversion rare earth photophysics is demonstrated for the first time. This work describes in detail the influence of the interference modulation on both the excitation (interion energy transfer) and radiative decay with nonradiative decay processes active between emissive levels...
December 2, 2016: Small
https://www.readbyqxmd.com/read/27911417/plasma-assisted-molecular-beam-epitaxy-of-n-polar-inaln-barrier-high-electron-mobility-transistors
#3
Matthew T Hardy, David F Storm, D Scott Katzer, Brian P Downey, Neeraj Nepal, David J Meyer
Plasma-assisted molecular beam epitaxy is well suited for the epitaxial growth of III-nitride thin films and heterostructures with smooth, abrupt interfaces required for high-quality high-electron-mobility transistors (HEMTs). A procedure is presented for the growth of N-polar InAlN HEMTs, including wafer preparation and growth of buffer layers, the InAlN barrier layer, AlN and GaN interlayers and the GaN channel. Critical issues at each step of the process are identified, such as avoiding Ga accumulation in the GaN buffer, the role of temperature on InAlN compositional homogeneity, and the use of Ga flux during the AlN interlayer and the interrupt prior to GaN channel growth...
November 24, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911401/an-optimized-protocol-to-analyze-glycolysis-and-mitochondrial-respiration-in-lymphocytes
#4
Javier Traba, Pietro Miozzo, Billur Akkaya, Susan K Pierce, Munir Akkaya
Lymphocytes respond to a variety of stimuli by activating intracellular signaling pathways, which in turn leads to rapid cellular proliferation, migration and differentiation, and cytokine production. All of these events are tightly linked to the energy status of the cell, and therefore studying the energy-producing pathways may give clues about the overall functionality of these cells. The extracellular flux analyzer is a commonly used device for evaluating the performance of glycolysis and mitochondrial respiration in many cell types...
November 21, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911393/a-detailed-protocol-for-perspiration-monitoring-using-a-novel-small-wireless-device
#5
Kazuhiro Ogai, Masakazu Fukuoka, Kei-Ichiro Kitamura, Kiyoshi Uchide, Tetsu Nemoto
Perspiration monitoring can be utilized for the detection of certain diseases, such as thermoregulation and mental disorders, particularly when the patients are unaware of such disorders or are having difficulty expressing their symptoms. Until now, several devices for perspiration monitoring have been developed; however, such devices tend to have a relatively large exterior, considerable power consumption, and/or less sensitivity. Recently, we developed a small, wireless device for perspiration monitoring...
November 24, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911382/a-simple-mechanical-procedure-to-create-limbal-stem-cell-deficiency-in-mouse
#6
Neda Afsharkhamseh, Elham Ghahari, Medi Eslani, Ali R Djalilian
Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with conjunctiva. Patients suffer from recurrent corneal defects, pain, inflammation, and loss of vision. Previously, a murine model of LSCD was described and compared to two other models. The goal was to produce a consistent mouse model of LSCD that both mimics the phenotype in humans and lasts long enough to make it possible to study the disease pathophysiology and to evaluate new treatments...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911381/a-novel-approach-for-the-administration-of-medications-and-fluids-in-emergency-scenarios-and-settings
#7
Akilesh Honasoge, Neal Lyons, Kathleen Hesse, Braden Parker, Robert Mokszycki, Kelly Wesselhoff, Rolla Sweis, Erik B Kulstad
The available routes of administration commonly used for medications and fluids in the acute care setting are generally limited to oral, intravenous, or intraosseous routes, but in many patients, particularly in the emergency or critical care settings, these routes are often unavailable or time-consuming to access. A novel device is now available that offers an easy route for administration of medications or fluids via rectal mucosal absorption (also referred to as proctoclysis in the case of fluid administration and subsequent absorption)...
November 9, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911375/fabrication-of-white-light-emitting-electrochemical-cells-with-stable-emission-from-exciplexes
#8
Soichi Uchida, Daisuke Takizawa, Satoru Ikeda, Hironori Takeuchi, Suzushi Nishimura, Hiroyuki Nishide, Yoshinori Nishikitani
The authors present an approach for fabricating stable white light emission from polymer light-emitting electrochemical cells (PLECs) having an active layer which consists of blue-fluorescent poly(9,9-di-n-dodecylfluorenyl-2,7-diyl) (PFD) and π-conjugated triphenylamine molecules. This white light emission originates from exciplexes formed between PFD and amines in electronically excited states. A device containing PFD, 4,4',4''-tris[2-naphthyl(phenyl)amino]triphenylamine (2-TNATA), Poly(ethylene oxide) and K2CF3SO3 showed white light emission with Commission internationale de l'éclairage (CIE) coordinates of (0...
November 15, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911352/medical-equipment-antiseptic-processes-using-the-atmospheric-plasma-sprayed-copper-coatings
#9
M Goudarzi, Sh Saviz, M Ghoranneviss, A Salar Elahi
Antibacterial surfaces such as copper coatings are able to reduce the growth of bacteria. In this study, copper coatings was deposited on the 316 stainless steel substrates by a handmade device operating as an atmospheric plasma spraying system. The chemical composition microstructure and morphology surface of the coatings are examined by x-ray diffraction (XRD) and scanning electron microscope (SEM) and back scattering electron microscope (BSE). Micro hardness as one of the key properties of the coating is characterized based on cross-section...
November 30, 2016: Journal of X-ray Science and Technology
https://www.readbyqxmd.com/read/27911347/smartphone-applications-for-immersive-virtual-reality-therapy-for-internet-addiction-and-internet-gaming-disorder
#10
Melvyn W B Zhang, Roger C M Ho
There have been rapid advances in technologies over the past decade and virtual reality technology is an area which is increasingly utilized as a healthcare intervention in many disciplines including that of Medicine, Surgery and Psychiatry. In Psychiatry, most of the current interventions involving the usage of virtual reality technology is limited to its application for anxiety disorders. With the advances in technology, Internet addiction and Internet gaming disorders are increasingly prevalent. To date, these disorders are still being treated using conventional psychotherapy methods such as cognitive behavioural therapy...
November 25, 2016: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
https://www.readbyqxmd.com/read/27911134/split-face-histological-and-biochemical-evaluation-of-tightening-efficacy-using-temperature-and-impedance-controlled-continuous-non-invasive-radiofrequency-energy
#11
Sylvie Boisnic, Marc Divaris, Marie-Christine Branchet, Andrew Anthony Nelson
BACKGROUND: Bi-polar radiofrequency (RF) is capable of heating dermal collagen fibers and inducing skin tightening by collagen remodelling. OBJECTIVE: To substantiate safety and improvement of skin laxity following skin heating with a novel temperature and impedance controlled non-invasive radiofrequency (RF) device by histological and biochemical evaluations. METHODS: A split-face study was performed on 4 subjects that underwent 8 weekly RF sessions on one side of their face, leaving the other side an untreated control and then underwent face-lift procedure...
December 2, 2016: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/27911043/a-cyclic-alkyl-amino-carbene-as-two-atom-%C3%AF-chromophore-leading-to-the-first-phosphorescent-linear-cu-i-complexes
#12
Markus Gernert, Ulrich Müller, Martin Haehnel, Jens Pflaum, Andreas Steffen
A series of linear and trigonal copper(I) complexes bearing a cyclic alkyl(amino)carbene (CAAC) ligand show surprising photophysical properties when compared to their corresponding N-heterocyclic carbene (NHC) complexes. Whereas the linear NHC complexes [CuX(NHC)] are nearly non-emissive, [CuX(CAAC)] (X = Cl, Br, I) and [Cu(CAAC)2]PF6 show very bright emission from their triplet excited states in the blue to green region displaying quantum yields of up to 65% in the solid state, although the π-acceptor comprises only the carbene C and N atoms with no other π conjugation...
December 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27910958/switchable-3d-optofluidic-y-branch-waveguides-tuned-by-dean-flows
#13
L Li, X Q Zhu, L Liang, Y F Zuo, Y S Xu, Y Yang, Y J Yuan, Q Q Huang
Optical branch waveguides are one of the most important optical elements and have been widely exploited for optical communication systems. However, prevailing devices are typically solid and have limit in tunability. Liquid optical devices have attracted more interest for the advantage of tunability of liquid media, but their signals suffer serious leakage if the refractive index (RI) of liquid is smaller than that of solid channels. This paper demonstrates the tunable three-dimensional (3D) optofluidic Y-branch waveguides in plannar microchannels by simply introducing Dean flow...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910941/rapid-mapping-of-polarization-switching-through-complete-information-acquisition
#14
Suhas Somnath, Alex Belianinov, Sergei V Kalinin, Stephen Jesse
Polarization switching in ferroelectric and multiferroic materials underpins a broad range of current and emergent applications, ranging from random access memories to field-effect transistors, and tunnelling devices. Switching in these materials is exquisitely sensitive to local defects and microstructure on the nanometre scale, necessitating spatially resolved high-resolution studies of these phenomena. Classical piezoresponse force microscopy and spectroscopy, although providing necessary spatial resolution, are fundamentally limited in data acquisition rates and energy resolution...
December 2, 2016: Nature Communications
https://www.readbyqxmd.com/read/27910921/bipolar-and-unipolar-silylene-diphenylene-%C3%AF-%C3%AF-conjugated-polymer-route-for-highly-efficient-electrophosphorescence
#15
Yao-Tang Chang, Sunil Sharma, Miao-Ken Hung, Yu-Hsuan Lee, Show-An Chen
σ-π conjugated polymer strategy is proposed for designing electroluminescent host polymers with silylene-diphenylene as the backbone repeat unit giving a high triplet energy (ET = 2.67 eV). By incorporation of high ET (3.0 eV) electron (oxadiazole, OXD) and hole (triphenyl amine, TPA) transport moieties, or TPA alone (in this case, the main chain acts as electron transport channel) as side arms on the silylene, the high ET bipolar and unipolar polymers are formed, allowing a use of iridium green phosphor (Ir(ppy)2(acac), Ir-G) (ET = 2...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910918/crescent-shaped-fabry-perot-fiber-cavity-for-ultra-sensitive-strain-measurement
#16
Ye Liu, D N Wang, W P Chen
Optical Fabry-Perot interferometer sensors based on inner air-cavity is featured with compact size, good robustness and high strain sensitivity, especially when an ultra-thin air-cavity is adopted. The typical shape of Fabry-Perot inner air-cavity with reflection mode of operation is elliptic, with minor axis along with and major axis perpendicular to the fiber length. The first reflection surface is diverging whereas the second one is converging. To increase the visibility of the output interference pattern, the length of major axis should be large for a given cavity length...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910889/direct-imaging-of-defect-formation-in-strained-organic-flexible-electronics-by-scanning-kelvin-probe-microscopy
#17
Tobias Cramer, Lorenzo Travaglini, Stefano Lai, Luca Patruno, Stefano de Miranda, Annalisa Bonfiglio, Piero Cosseddu, Beatrice Fraboni
The development of new materials and devices for flexible electronics depends crucially on the understanding of how strain affects electronic material properties at the nano-scale. Scanning Kelvin-Probe Microscopy (SKPM) is a unique technique for nanoelectronic investigations as it combines non-invasive measurement of surface topography and surface electrical potential. Here we show that SKPM in non-contact mode is feasible on deformed flexible samples and allows to identify strain induced electronic defects...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910871/noncontact-and-wide-field-characterization-of-the-absorption-and-scattering-properties-of-apple-fruit-using-spatial-frequency-domain-imaging
#18
Dong Hu, Xiaping Fu, Xueming He, Yibin Ying
Spatial-frequency domain imaging (SFDI), as a noncontact, low-cost and wide-field optical imaging technique, offers great potential for agro-product safety and quality assessment through optical absorption (μa) and scattering (μ) property measurements. In this study, a laboratory-based SFDI system was constructed and developed for optical property measurement of fruits and vegetables. The system utilized a digital light projector to generate structured, periodic light patterns and illuminate test samples...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910861/paper-based-crp-monitoring-devices
#19
Shang-Chi Lin, Chung-Yuh Tseng, Po-Liang Lai, Min-Yen Hsu, Shueh-Yao Chu, Fan-Gang Tseng, Chao-Min Cheng
Here, we discuss the development of a paper-based diagnostic device that is inexpensive, portable, easy-to-use, robust, and capable of running simultaneous tests to monitor a relevant inflammatory protein for clinical diagnoses i.e. C-reactive protein (CRP). In this study, we first attempted to make a paper-based diagnostic device via the wax printing method, a process that was used in previous studies. This device has two distinct advantages: 1) reduced manufacturing and assay costs and operation duration via using wax printing method to define hydrophobic boundaries (for fluidic devices or general POC devices); and, 2) the hydrophilicity of filter paper, which is used to purify and chromatographically correct interference caused by whole blood components with a tiny amount of blood sample (only 5 μL)...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910852/kaleidoscopic-imaging-patterns-of-complex-structures-fabricated-by-laser-induced-deformation
#20
Haoran Zhang, Fengyou Yang, Jianjie Dong, Lena Du, Chuang Wang, Jianming Zhang, Chuan Fei Guo, Qian Liu
Complex surface structures have stimulated a great deal of interests due to many potential applications in surface devices. However, in the fabrication of complex surface micro-/nanostructures, there are always great challenges in precise design, or good controllability, or low cost, or high throughput. Here, we present a route for the accurate design and highly controllable fabrication of surface quasi-three-dimensional (quasi-3D) structures based on a thermal deformation of simple two-dimensional laser-induced patterns...
December 2, 2016: Nature Communications
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