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Hepatocyte proliferation

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https://www.readbyqxmd.com/read/29240284/extracellular-matrix-stromal-vascular-fraction-gel-conditioned-medium-accelerates-wound-healing-in-a-murine-model
#1
Chengliang Deng, Yunfan He, Jingwei Feng, Ziqing Dong, Yao Yao, Hsiaopei Mok, Maohui Lin, Lu Feng
Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown a successful outcome in wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), an adipose-derived stem cell and adipose native extracellular matrix-enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF-gel (Gel-CM) on wound healing compared with the conventional CM from adipose tissue (Adi-CM) and stem cell (SVF-CM). In vitro wound healing effect of three CMs on keratinocytes and fibroblasts was evaluated in terms of proliferation property, migratory property, and extracellular matrix production...
December 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29236324/silencing-of-phospholipase-c-gamma-2-promotes-proliferation-of-rat-hepatocytes-in-vitro
#2
Xiaoguang Chen, Xuemin Zhu, Yumei Liu, Qiongxia Lv, Jun Ma
The management of hepatic failure is undoubtedly difficult, and poor results have led to the search for novel therapeutic approaches. Nowadays, anti-apoptotic gene therapy is considered as an ideal approach. It has been proved that phospholipase Cγ2 (PLCγ2) is involved in the apoptosis of immune cells and tumor cells; however, whether this gene is related to hepatocyte death is still unclear. This study examined the role of PLCγ2 by inhibiting its expression in rat hepatocytes with siRNA. We also further analyzed the cellular mechanism by which the expression inhibition of PLCγ2 induces cell death...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29234104/conjunction-of-potential-g-quadruplex-and-adjacent-cis-elements-in-the-5-utr-of-hepatocyte-nuclear-factor-4-alpha-strongly-inhibit-protein-expression
#3
Shangdong Guo, Hong Lu
Hepatocyte nuclear factor 4-alpha (HNF4α) is a well established master regulator of liver development and function. We identified the in vitro presence of a stable secondary structure, G-quadruplex (G4) in the 5' UTR of P1-HNF4A, the predominant HNF4α isoform(s) in adult liver. Our data suggest that the cooperation of G4 and the adjacent putative protein-binding sites within the 5' UTR was necessary and sufficient to mediate a strong translational repression. This was supported by analysis of deleted/mutated 5'UTRs and two native regulatory single-nucleotide polymorphisms in the 5'UTR...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29231907/activated-hgf-c-met-axis-in-head-and-neck-cancer
#4
REVIEW
Levi Arnold, Jonathan Enders, Sufi Mary Thomas
Head and neck squamous cell carcinoma (HNSCC) is a highly morbid disease. Recent developments including Food and Drug Administration (FDA) approved molecular targeted agent's pembrolizumab and cetuximab show promise but did not improve the five-year survival which is currently less than 40%. The hepatocyte growth factor receptor; also known as mesenchymal-epithelial transition factor (c-Met) and its ligand hepatocyte growth factor (HGF) are overexpressed in head and neck squamous cell carcinoma (HNSCC); and regulates tumor progression and response to therapy...
December 12, 2017: Cancers
https://www.readbyqxmd.com/read/29225769/fabrication-of-low-cost-micropatterned-polydimethyl-siloxane-scaffolds-to-organise-cells-in-a-variety-of-two-dimensioanl-biomimetic-arrangements-for-lab-on-chip-culture-platforms
#5
Lidia Escutia-Guadarrama, Genaro Vázquez-Victorio, David Martínez-Pastor, Brenda Nieto-Rivera, Marcela Sosa-Garrocho, Marina Macías-Silva, Mathieu Hautefeuille
We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29223705/functional-hepatocyte-clusters-on-bioactive-blend-silk-matrices-towards-generating-bioartificial-liver-constructs
#6
Guru Janani, Samit K Nandi, Biman B Mandal
The creation of in vitro functional hepatic tissue simulating micro-environmental niche of native liver is a keen area of research due to its demand in bioartificial liver (BAL) and cell-based tissue engineering. Here, we investigated the potential of novel blend (BA) silk scaffold fabricated by blending mulberry (Bombyx mori, BM) silk fibroin with cell adhesion motif (RGD) rich non-mulberry (Antheraea assamensis, AA) silk fibroin in generating a functional liver construct. Three-dimensional (3D) porous silk scaffolds (BM, AA and BA) were physico-chemically characterized and functionally evaluated using human hepatocarcinoma cells (HepG2) and primary neonatal rat hepatocytes...
December 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29217749/hepatitis-delta-virus-persists-during-liver-regeneration-and-is-amplified-through-cell-division-both-in-vitro-and-in-vivo
#7
Katja Giersch, Oliver D Bhadra, Tassilo Volz, Lena Allweiss, Kristoffer Riecken, Boris Fehse, Ansgar W Lohse, Joerg Petersen, Camille Sureau, Stephan Urban, Maura Dandri, Marc Lütgehetmann
OBJECTIVE: Hepatitis delta virus (HDV) was shown to persist for weeks in the absence of HBV and for months after liver transplantation, demonstrating the ability of HDV to persevere in quiescent hepatocytes. The aim of the study was to evaluate the impact of cell proliferation on HDV persistence in vitro and in vivo. DESIGN: Genetically labelled human sodium taurocholate cotransporting polypeptide (hNTCP)-transduced human hepatoma(HepG2) cells were infected with HBV/HDV and passaged every 7 days for 100 days in the presence of the entry inhibitor Myrcludex-B...
December 7, 2017: Gut
https://www.readbyqxmd.com/read/29211918/decellularized-human-placenta-supports-hepatic-tissue-and-allows-rescue-in-acute-liver-failure
#8
Zurab Kakabadze, Anne Kakabadze, David Chakhunashvili, Lika Karalashvili, Ekaterine Berishvili, Yogeshwar Sharma, Sanjeev Gupta
Tissue engineering with scaffolds to form transplantable organs is of wide interest. Decellularized tissues have been tested for this purpose, although supplies of healthy donor tissues, vascular recellularization for perfusion and tissue homeostasis in engineered organs pose challenges. We hypothesized decellularized human placenta will be suitable for tissue engineering. The universal availability and unique structures of placenta for accommodating tissue, including presence of embedded vessels, were major attractions...
December 6, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29211363/hierarchical-potential-differentiation-of-liver-cancer-stem-cells
#9
REVIEW
Wenfeng Zhang, Di Mu, Kai Feng
Hepatocellular carcinoma (HCC) is one of the most malignant tumors in Chinese people and offers poor prognosis. Tumor tissue, like normal tissue, is hierarchically differentiated. Thus, minor tumor cell populations able to differentiate, such as stem cells, sustain tumor self-renewal and proliferation. The fact that liver cancer stem cells (CSCs) with different surface markers appear heterogeneous with respect to oncogenesis and drug resistance indicates that subpopulations of surface markers preserve the hierarchical potential of differentiation during proliferation, deterioration and relapse...
October 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29208510/differential-impacts-of-individual-and-combined-exposures-of-deoxynivalenol-and-zearalenone-on-the-heparg-human-hepatocyte-proteome
#10
Marie-Caroline Smith, Emma Timmins-Schiffman, Monika Coton, Emmanuel Coton, Nolwenn Hymery, Brook L Nunn, Stéphanie Madec
Numerous surveys have highlighted the natural co-occurrence of deoxynivalenol (DON) and zearalenone (ZEA) mycotoxins in food and feed. Nevertheless, data regarding cellular mechanisms involved in response to their individual and simultaneous exposures are lacking. In this study, in order to analyze how low mycotoxin doses could impact cellular physiology and homeostasis, proteomic profiles of proliferating human hepatocytes (HepaRG) exposed for 1h and 24h to low DON and ZEA cytotoxicity levels (0.2 and 20μM respectively), alone or in combination, were analyzed by LC-MS/MS...
December 2, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29207650/upregulation-of-mir-181a-impairs-hepatic-glucose-and-lipid-homeostasis
#11
Xiliang Du, Yuchen Yang, Chuang Xu, Zhicheng Peng, Min Zhang, Lin Lei, Wenwen Gao, Yuhao Dong, Zhen Shi, Xudong Sun, Zhe Wang, Xiaobing Li, Xinwei Li, Guowen Liu
The contributions of altered post-transcriptional gene silencing to the development of metabolic disorders remain poorly understood thus far. The objective of this study was to evaluate the roles of miR-181a in the regulation of hepatic glucose and lipid metabolism. MiR-181a is abundantly expressed in the liver, and we found that blood and hepatic miR-181a levels were significantly increased in patients and dairy cows with non-alcoholic fatty liver disease, as well as in high-fat diet and ob/ob mice. We determined that sirtuin1 is a target of miR-181a...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29207082/autophagy-regulates-proliferation-and-biliary-differentiation-of-hepatic-oval-cells-via-the-mapk-erk-signaling-pathway
#12
Dongdong Chen, Xiaoxiao Wu, Jie Zheng, Ruijie Dai, Zhichao Mo, Fahad Munir, Xiaolong Ni, Yunfeng Shan
Hepatic oval cells (HOCs) are thought to possess self‑renewal ability and a bipotential capacity for differentiation, which allows them to differentiate into hepatocytes and cholangiocytes. Autophagy serves an important role in self‑renewal and differentiation of stem cells; however, how autophagy contributes to proliferation and differentiation of hepatic progenitor cells has yet to be elucidated. In the present study, autophagy was regulated by rapamycin (Rapa) and chloroquine (Chlo) administration. The results demonstrated that Chlo‑treated HOCs exhibited decreased autophagic activity alongside a decreased tendency to proliferate, as determined by Cell Counting Kit‑8...
November 27, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29206210/immortalization-of-porcine-11%C3%AE-hydroxysteroid-dehydrogenase-type-1-transgenic-liver-cells-using-sv40-large-t-antigen
#13
Hee Young Kang, Young-Kwon Choi, Yeon Ik Jeong, Kyung-Chul Choi, Sang-Hwan Hyun, Woo-Suk Hwang, Eui-Bae Jeung
Cortisol is a steroid hormone essential to the maintenance of homeostasis that is released in response to stress and low blood glucose concentration. Cortisol is converted from cortisone by 11βhydroxysteroid dehydrogenase type 1 (HSD11B1). It has been reported that too much cortisol or overexpression of HSD11B1 induces obesity and the insulin resistance that accompanies metabolic syndrome in rodent adipose tissue. In our previous study, HSD11B1-transgenic (TG) fibroblasts were established, and a porcine model was generated by SCNT using those fibroblasts...
December 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29205334/sodium-glucose-cotransporter-2-inhibitor-canagliflozin-attenuates-liver-cancer-cell-growth-and-angiogenic-activity-by-inhibiting-glucose-uptake
#14
Kosuke Kaji, Norihisa Nishimura, Kenichiro Seki, Shinya Sato, Soichiro Saikawa, Keisuke Nakanishi, Masanori Furukawa, Hideto Kawaratani, Mitsuteru Kitade, Kei Moriya, Tadashi Namisaki, Hitoshi Yoshiji
Sodium-glucose cotransporter 2 inhibitors (SGLT2-Is) comprise a new class of antidiabetic agents that inhibit glucose reabsorption in the renal proximal tubules. Although a recent report demonstrated the potential ability of SGLT2-Is to attenuate cancer growth of SGLT2-expressing cancer cells, little is known about the effects of SGLT2-Is on hepatocellular carcinoma (HCC). Here, we investigate the anti-cancer properties of a SGLT2-I, canagliflozin, against human liver cancer cells. SGTL2 mRNA and protein expression were detected in Huh7 and HepG2 cells, although not in HLE as well as primary human hepatocytes and hepatic stellate cells...
December 4, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29204978/role-of-glypican-3-in-the-growth-migration-and-invasion-of-primary-hepatocytes-isolated-from-patients-with-hepatocellular-carcinoma
#15
Mauro Montalbano, Cristiana Rastellini, Joshua T McGuire, Janika Prajapati, Ali Shirafkan, Renza Vento, Luca Cicalese
BACKGROUND: Recently, Glypican-3 (GPC3) has been identified as a potential hepatocellular carcinoma (HCC) diagnostic and/or therapeutic target. GPC3 has been found to be up-regulated in HCC and to be absent in normal and cirrhotic liver. As yet, however, the molecular characteristics of GPC3 and its role in HCC cell physiology and development are still undefined. METHODS: Human hepatocyte cultures were established from 10 HCC patients. Additional liver samples were obtained from 5 patients without cirrhosis and/or HCC...
December 4, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29204817/oxyphenbutazone-promotes-cytotoxicity-in-rats-and-hep3b-cellsvia-suppression-of-pge2-and-deactivation-of-wnt-%C3%AE-catenin-signaling-pathway
#16
Shakir Saleem, Ruqaiyah Khan, Muhammad Afzal, Imran Kazmi
Hepatocellular carcinoma (HCC) is the fifth leading cause of death and is generally typified by elevated liver enzyme biomarkers, antioxidants, and chronic inflammation of hepatocytes. Although currently available drugs have shown remarkable alleviation of the cancerous condition, but at the same time they present a more severe challenge of toxic effects due to chemotherapy. Therefore, in order to bring more patient-compliant therapy, we aimed to refurbish the use of a COX inhibitor, oxyphenbutazone (OPB), with low dose of methotrexate (MTX) to treat diethyl nitrosamine (DENA)-induced HCC in Wistar rats and in Hep3B cells...
December 4, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29204746/comparative-analysis-of-human-wharton-s-jelly-mesenchymal-stem-cells-derived-from-different-parts-of-the-same-umbilical-cord
#17
Dinesh Bharti, Sharath Belame Shivakumar, Ji-Kwon Park, Imran Ullah, Raghavendra Baregundi Subbarao, Ji-Sung Park, Sung-Lim Lee, Bong-Wook Park, Gyu-Jin Rho
Easy isolation, lack of ethical issues, high proliferation, multi-lineage differentiation potential and immunomodulatory properties of umbilical cord (UC)-derived mesenchymal stem cells (MSCs) make them a valuable tool in stem cell research. Recently, Wharton's jelly (WJ) was proven as the best MSC source among various compartments of UC. However, it is still unclear whether or not Wharton's jelly-derived MSCs (WJMSCs) from different parts of the whole cord exhibit the same characteristics. There may be varied MSCs present in different parts of WJ throughout the length of the UC...
December 4, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29200956/stem-cells-from-cryopreserved-human-dental-pulp-tissues-sequentially-differentiate-into-definitive-endoderm-and-hepatocyte-like-cells-in-vitro
#18
Young-Jin Han, Young-Hoon Kang, Sarath Belame Shivakumar, Dinesh Bharti, Young-Bum Son, Yong-Ho Choi, Won-Uk Park, June-Ho Byun, Gyu-Jin Rho, Bong-Wook Park
We previously described a novel tissue cryopreservation protocol to enable the safe preservation of various autologous stem cell sources. The present study characterized the stem cells derived from long-term cryopreserved dental pulp tissues (hDPSCs-cryo) and analyzed their differentiation into definitive endoderm (DE) and hepatocyte-like cells (HLCs) in vitro. Human dental pulp tissues from extracted wisdom teeth were cryopreserved as per a slow freezing tissue cryopreservation protocol for at least a year...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29197930/the-ppar%C3%AE-dependent-rodent-liver-tumor-response-is-not-relevant-to-humans-addressing-misconceptions
#19
REVIEW
J Christopher Corton, Jeffrey M Peters, James E Klaunig
A number of industrial chemicals and therapeutic agents cause liver tumors in rats and mice by activating the nuclear receptor peroxisome proliferator-activated receptor α (PPARα). The molecular and cellular events by which PPARα activators induce rodent hepatocarcinogenesis have been extensively studied elucidating a number of consistent mechanistic changes linked to the increased incidence of liver neoplasms. The weight of evidence relevant to the hypothesized mode of action (MOA) for PPARα activator-induced rodent hepatocarcinogenesis is summarized here...
December 2, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29197058/thiamethoxam-induced-hepatotoxicity-and-pro-carcinogenicity-in-rabbits-via-motivation-of-oxidative-stress-inflammation-and-anti-apoptotic-pathway
#20
Osama S El Okle, Omnia I El Euony, Asmaa F Khafaga, Mohamed A Lebda
Thiamethoxam (TMX) is a non-mutagenic neonicotinoid insecticide that is widely used to combat different types of insects. The hepatotoxicity and carcinogenicity of TMX have been approved previously in mice but not in rats. However, the TMX-induced hepatotoxic and pro-carcinogenic effects on rabbits remain unclear. The present study elucidated the roles of oxidative stress, pro-inflammatory cytokines, and apoptosis-related genes in the hepatotoxic and carcinogenic effects of TMX on rabbits. Sixteen male rabbits were equally divided into two groups; eight rabbits orally treated with TMX at a dose of 250 mg/kg b...
December 1, 2017: Environmental Science and Pollution Research International
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