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Hepatocyte proliferation

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https://www.readbyqxmd.com/read/29456739/effects-of-pha-665752-and-vemurafenib-combination-treatment-on-in-vitro-and-murine-xenograft-growth-of-human-colorectal-cancer-cells-with-braf-v600e-mutations
#1
Jie Zhi, Zhongxin Li, Jian Lv, Bo Feng, Donghai Yang, Liang Xue, Zhaolong Zhao, Yanni Zhang, Jianhua Wu, Yingchao Jv, Yitao Jia
It remains unknown whether blockade of B-Raf proto-oncogene, serine/threonine kinase (BRAF)V600E signaling and MET proto-oncogene, receptor tyrosine kinase (c-Met) signaling is effective in suppressing the growth of human colorectal cancer (CRC) cells. The present study investigated the effects of the vemurafenib alone and in combination with c-Met inhibitor PHA-665752 on the growth of human CRC cells in vitro and in mouse xenografts. HT-29 and RKO CRC cell lines with BRAFV600E mutations and mice bearing HT-29 xenografts were treated with vemurafenib in the absence or presence of PHA-665752...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29451151/exosomal-microrna-10a-is-associated-with-liver-regeneration-in-rats-through-downregulation-of-epha4
#2
Lin Luo, Ze-Ping Yu, Han Qin, Ze-Xin Zhu, Ming-Heng Liao, Hao-Tian Liao, Ke-Fei Yuan, Yong Zeng
Background: MicroRNAs (miRNAs) have been reported to play vital roles in liver regeneration. Previous studies mainly focused on the functions of intracellular miRNAs, while the functions of circulating exosomal miRNAs in liver regeneration remain largely unknown. The aim of this study was to identify the key exosomal miRNA that played vital roles in liver regeneration. Methods: The Sprague-Dawley male rats were assigned to 70% partially hepatectomized group (n = 6) and sham surgery group (n = 6)...
February 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29448959/impaired-t-cell-mediated-hepatitis-in-peroxisome-proliferator-activated-receptor-alpha-ppar%C3%AE-deficient-mice
#3
Ian N Hines, Michael Kremer, Sherri M Moore, Michael D Wheeler
BACKGROUND: Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). METHODS: Wild type (wt) or PPARα-deficient (PPARα -/- ) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization...
February 15, 2018: Biological Research
https://www.readbyqxmd.com/read/29448836/aav8-gene-therapy-rescues-the-newborn-phenotype-of-a-mouse-model-of-crigler-najjar
#4
Jenny A Greig, Jayme M L Nordin, Christine Draper, Peter Bell, James M Wilson
Adeno-associated viral (AAV) vectors can target the liver, making them an attractive platform for gene therapy approaches that require the correction of hepatocytes. Crigler-Najjar syndrome is an autosomal recessive disorder of bilirubin metabolism that occurs when the liver's uridine diphosphate glucuronosyl transferase 1A1 (UGT1A1) enzyme activity is partially or completely absent. This syndrome is characterized by elevated bilirubin levels in the blood. We developed an AAV8 vector expressing a codon-optimized human version of UGT1A1 from a liver-specific promoter...
February 16, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29445190/the-chemokine-receptor-ccr10-promotes-inflammation-driven-hepatocarcinogenesis-via-pi3k-akt-pathway-activation
#5
Qiong Wu, Jin-Xian Chen, Yu Chen, Li-Li Cai, Xiao-Zhong Wang, Wu-Hua Guo, Jian-Feng Zheng
G-protein-coupled receptor (GPCR)-related proteins are dysregulated and the GPCR CC-chemokine receptor 10 (CCR10) is significantly upregulated in inflammation-driven HCC. However, CCR10's role in inflammation-driven hepatocarcinogenesis remains unknown. The aim of this study was to evaluate the role of CCR10 in inflammation-driven hepatocarcinogenesis. Via a targeted gene expression microarray screening alterations in GPCR family gene expression, we found CCR10 to be significantly upregulated in hepatocytes isolated from inflammation-driven human HCC tumors and matching paracancerous tissues...
February 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29443890/embedded-disposable-functionalized-electrochemical-biosensor-with-a-3d-printed-flow-cell-for-detection-of-hepatic-oval-cells-hocs
#6
Samar Damiati, Martin Peacock, Stefan Leonhardt, Laila Damiati, Mohammed A Baghdadi, Holger Becker, Rimantas Kodzius, Bernhard Schuster
Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describes the development of a 3D-printed continuous flow system and exploits disposable screen-printed electrodes for the rapid detection of HOCs that over-express the OV6 marker on their membrane. Multiwall carbon nanotube (MWCNT) electrodes have a chitosan film that serves as a scaffold for the immobilization of oval cell marker antibodies (anti-OV6-Ab), which enhance the sensitivity of the biomarker and makes the designed sensor specific for oval cells...
February 14, 2018: Genes
https://www.readbyqxmd.com/read/29442770/analysis-of-colchicine-induced-effects-on-hepatoma-and-hepatocyte-cells-by-atomic-force-microscopy
#7
Lanjiao Liu, Zuobin Wang, Wenxiao Zhang, Xinyao Zhu, Li Li, Zhankun Weng
Biomechanical properties of cells are altered by many diseases. Cancer cell metastasis is related to the properties such as the cell stiffness that influences cell proliferation, differentiation and migration. In this paper, we used an atomic force microscope to analyze the colchicine-induced effects on the mechanical properties of hepatocyte (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture at the nanoscale. The cells were exposed to a solution with a normal dose of colchicine for two, four and six hours...
June 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442042/trim59-promotes-cell-proliferation-migration-and-invasion-in-human-hepatocellular-carcinoma-cells
#8
Gang Sun, Xin Sui, Dayue Han, Jianjun Gao, Yining Liu, Lin Zhou
The human tripartite motif (TRIM) 59 has been implicated in tumorigenesis of many types of cancer. However, the biological function and molecular mechanism of TRIM59 in hepatocellular carcinoma (HCC) remains unknown. In our study, the purpose was to investigate the impact of TRIM59 on the biologic behavior of HCC cells. We observed that TRIM59 was highly expressed in HCC cells compared with a normal human hepatocyte cell line. Lentivirus-mediated knocking down of TRIM59 significantly suppressed the proliferation, migration and invasion of HCC cells, whereas overexpression of TRIM59 enhanced cell growth and metastasis...
November 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29435697/establishment-and-characterization-of-an-immortalized-human-hepatocyte-line-for-the-development-of-bioartificial-liver-system
#9
Qun Yan, Lijuan Deng, Xinmei Zhao, Liangying Ye, Yuxin Fang, Yan Meng, Zenan Wang, Xiaobei Luo, Side Liu, Aimin Li
For further research and application of Bioartificial liver systems (BAL), active proliferation capacity and full hepatic functionality of the biomaterials is mandatory. However, there are still no suitable cell lines meeting the requirements for an ideal cell source in BAL development that makes it necessary to explore other sources. Here, we constructed a new cell line derived from well-differentiated hepatocellular carcinoma tissues designated NHBL2. Immunol staining showed that NHBL2 possessed the capacity of synthesizing albumin and CYP2E1 and quantitative analysis showed that the albumin synthesis ability of NHBL2 was comparable to C3A while urea production was highly abundant of NHBL2 compared with that of C3A...
February 12, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29435059/macc1-and-hgf-are-associated-with-survival-in-patients-with-gastric-cancer
#10
Guokai Dong, Man Wang, Guangfu Gu, Shanshan Li, Xiaoming Sun, Zhouru Li, Hongxing Cai, Zhengqiu Zhu
Metastasis-associsated in colon cancer 1 (MACC1), a newly identified oncogene, promotes tumor cell proliferation and invasion. In the present study, the expression of MACC1, hepatocyte growth factor (HGF) and its receptor, MET proto-oncogene (c-Met), was investigated in human gastric cancer tissues and adjacent normal tissues by immunohistochemistry. The association between the expression levels of the proteins and the clinicopathological parameters of the tumors were statistically analyzed. Furthermore, lentiviral particles expressing MACC1 were used to infect the hepatic satellite cell (HSC) line LX2...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434765/platelet-rich-plasma-has-beneficial-effects-in-mice-with-osteonecrosis-of-the-femoral-head-by-promoting-angiogenesis
#11
Shichao Tong, Jimin Yin, Ji Liu
Platelet-rich plasma (PRP) is autologous and multifunctional. Platelet concentrate from blood contains highly concentrated platelets and various types of cells, including growth factors. PRP promotes the recovery of cell proliferation and differentiation. Osteonecrosis of the femoral head is a disease caused by femoral head damage or an insufficient blood supply, which leads to the death of bone cells and abnormal bone marrow composition. The subsequent repair of bone cells may result in changes to the structure of femoral head, femoral head collapse and joint dysfunction...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29434327/iterative-feedback-bio-printing-derived-cell-laden-hydrogel-scaffolds-with-optimal-geometrical-fidelity-and-cellular-controllability
#12
Ling Wang, Ming-En Xu, Li Luo, Yongyong Zhou, Peijian Si
For three-dimensional bio-printed cell-laden hydrogel tissue constructs, the well-designed internal porous geometry is tailored to obtain the desired structural and cellular properties. However, significant differences often exist between the designed and as-printed scaffolds because of the inherent characteristics of hydrogels and cells. In this study, an iterative feedback bio-printing (IFBP) approach based on optical coherence tomography (OCT) for the fabrication of cell-laden hydrogel scaffolds with optimal geometrical fidelity and cellular controllability was proposed...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29434311/directed-differentiation-of-adult-liver-derived-mesenchymal-like-stem-cells-into-functional-hepatocytes
#13
Xiaobei Luo, Kapish Gupta, Abhishek Ananthanarayanan, Zenan Wang, Lei Xia, Aimin Li, Rashidah Binte Sakban, Side Liu, Hanry Yu
Shortage of functional hepatocytes hampers drug safety testing and therapeutic applications because mature hepatocytes cannot be expanded and maintain functions in vitro. Recent studies have reported that liver progenitor cells can originate from mature hepatocytes in vivo. Derivation of proliferating progenitor cells from mature hepatocytes, and re-differentiation into functional hepatocytes in vitro has not been successful. Here we report the derivation of novel mesenchymal-like stem cells (arHMSCs) from adult rat hepatocytes...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29433675/plant-natural-product-plumbagin-presents-potent-inhibitory-effect-on-human-cytochrome-p450-2j2-enzyme
#14
Jian Lu, Daozhi Liu, Xiaojing Zhou, Ang Chen, Zhenran Jiang, Xiyun Ye, Mingyao Liu, Xin Wang
BACKGROUND: Cytochrome P450 2J2 (CYP2J2) is not only highly expressed in many kinds of human tumors, but also promotes tumor cell growth via regulating the metabolism of arachidonic acids. CYP2J2 inhibitors can significantly reduce proliferation, migration and promote apoptosis of tumor cells by inhibiting epoxyeicosatrienoic acids (EETs) biosynthesis. Therefore screening CYP2J2 inhibitors is a significant way for the development of anti-cancer drug. PURPOSE: The aim of this study was to identify a new CYP2J2 inhibitor from fifty natural compounds obtained from plants...
January 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29430764/plc%C3%AE-2-promotes-apoptosis-while-inhibits-proliferation-in-rat-hepatocytes-through-pkcd-jnk-mapk-and-pkcd-p38-mapk-signalling
#15
Xiaoguang Chen, Qiongxia Lv, Jun Ma, Yumei Liu
OBJECTIVES: The PLCG2 (PLCγ2) gene is a member of PLC gene family encoding transmembrane signalling enzymes involved in various biological processes including cell proliferation and apoptosis. Our earlier study indicated that PLCγ2 may be involved in the termination of regeneration of the liver which is mainly composed of hepatocytes, but its exact biological function and molecular mechanism in liver regeneration termination remains unclear. This study aims to examine the role of PLCγ2 in the growth of hepatocytes...
February 11, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29425642/effect-of-dioscin-on-promoting-liver-regeneration-via-activating-notch1-jagged1-signal-pathway
#16
Lina Xu, Lina Gu, Xufeng Tao, Youwei Xu, Yan Qi, Lianhong Yin, Xu Han, Jinyong Peng
BACKGROUND: Development of novel candidates to promote liver regeneration is critical important after partial hepatectomy (PH). Dioscin, a natural product, shows potent effect on liver protection in our previous works. PURPOSE: This work aimed to investigate the effect and underlying mechanisms of dioscin on liver regeneration. METHODS: The promoting proliferation effects of dioscin on mouse hepatocytem AML12 cells, rat primary hepatocytes, rats and mice after 70% PH were evaluated...
January 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29424143/-analysis-of-transcriptional-factors-expression-profile-during-dedifferentiation-of-liver-cell-cultured-in-vitro
#17
Ruiyang Li, Yunzhi Wang, Rui Ge, Wenhao Shi, Chen Ding
Primary hepatocytes are widely used in drug metabolism and toxicity assessment. As the culture of primary hepatocytes in vitro is a process of dedifferentiation, hepatocytes lose normal metabolic detoxification function gradually. The mechanism of hepatocyte dedifferentiation has been not clear so far. TFs play an important role in the dedifferentiation and non-parenchymal cells can maintain the function of hepatocytes in vitro. However, the current methods cannot be used in effective identification and quantitative analysis of a large number of TFs...
February 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29420858/endothelial-notch-activation-reshapes-the-angiocrine-of-sinusoidal-endothelia-to-aggravate-liver-fibrosis-and-blunt-regeneration
#18
Juan-Li Duan, Bai Ruan, Xian-Chun Yan, Liang Liang, Ping Song, Zi-Yan Yang, Yuan Liu, Ke-Feng Dou, Hua Han, Lin Wang
Liver sinusoidal endothelial cells (LSECs) critically regulate liver homeostasis and diseases through angiocrine factors. Notch is critical in endothelial cells (ECs). In the current study, Notch signaling was activated by inducible EC-specific expression of the Notch intracellular domain (NIC). We found that endothelial Notch activation damaged liver homeostasis. Notch activation resulted in decreased fenestration and increased basement membrane, and a gene expression profile with decreased LSEC-associated genes and increased continuous EC-associated genes, suggesting LSEC dedifferentiation...
February 8, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29413617/functionally-coated-polyethersulfone-hollow-fiber-membranes-a-substrate-for-enhanced-hepg2-c3a-functions
#19
Surendra Kumar Verma, Akshay Modi, Atul Kumar Singh, Rohit Teotia, Sachin Kadam, Jayesh Bellare
Hollow fiber membrane (HFM) based liver assist systems are a life-saving bridge for patients until a donor organ is available for transplantation or until liver regeneration. However, liver cell attachment and functional maintenance on HFM surface is a major challenge in bio-artificial liver (BAL) support systems. In the present study, novel glutaraldehyde (GTA)-crosslinked gelatin (gel)-coated polyethersulfone (X-gel-PT) HFMs were manufactured using triple orifice spinneret by the dry-wet spinning method. HFMs were characterized for morphology, outer surface roughness, hydrophilicity, tensile strength, thermal stability, BET surface area and pore volume measurements, permeability and rejection...
February 2, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29410409/exosome-mimetic-nanovesicles-from-hepatocytes-promote-hepatocyte-proliferation-in-vitro-and-liver-regeneration-in-vivo
#20
Jun-Yi Wu, An-Lai Ji, Zhong-Xia Wang, Guang-Hui Qiang, Zhen Qu, Jun-Hua Wu, Chun-Ping Jiang
The liver has great regenerative capacity after functional mass loss caused by injury or disease. Many studies have shown that primary hepatocyte-derived exosomes, which can deliver biological information between cells, promote the regenerative process of the liver. However, the yield of exosomes is very limited. Recent studies have demonstrated that exosome-mimetic nanovesicles (NVs) can be prepared from cells with almost 100 times the production yield compared with exosomes. Thus, this study investigated the therapeutic capacity of exosome-mimetic NVs from primary hepatocytes in liver regeneration...
February 6, 2018: Scientific Reports
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