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periodontal tissue engineering

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https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#1
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29213288/microrna-214-suppresses-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-by-targeting-atf4
#2
Siqi Yao, Wei Zhao, Qianmin Ou, Lanchen Liang, Xuefeng Lin, Yan Wang
Periodontitis is the main cause of adult tooth loss. Stem cell-based tissue engineering has become a promising therapy for periodontitis treatment. To date, human periodontal ligament stem cells (hPDLSCs) have been shown to be a favorable source for tissue engineering, but modulatory mechanisms of hPDLSCs remain unclear. Approximately 60% of mammalian genes are the targets of over 2000 miRNAs in multiple human cell types, and miRNAs are able to influence various biological processes in the human body, including bone formation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29201597/current-concepts-of-epigenetics-and-its-role-in-periodontitis
#3
REVIEW
Lena Larsson
Purpose: The focus of this review is to provide an overview of the recent findings on the role of epigenetic mechanisms in periodontal disease, including disease susceptibility, progression, and as potential treatment options. Recent Findings: The findings on the influence of oral pathogens on epigenetic regulation of pathogen recognition receptors, such as Toll-like receptors, as well as pro-inflammatory cytokines suggest an important role for epigenetics in the regulation of the host immune response...
2017: Current Oral Health Reports
https://www.readbyqxmd.com/read/29184628/comparison-of-in-vitro-properties-of-periodontal-ligament-stem-cells-derived-from-permanent-and-deciduous-teeth
#4
Masoumeh Khoshhal, Iraj Amiri, Leila Gholami
Background. Stem cells have contributed to the development of tissue-engineered-based regenerative periodontal therapies. In order to find the best stem cell sources for such therapies, the biologic properties of stem cells isolated from periodontal ligaments (PDL) of deciduous (DePDLSC) and permanent (PePDLSC) teeth were comparatively evaluated. Methods. PDL stem cells were isolated from six sound fully erupted premolars and six deciduous canines of healthy subjects. In vitro biologic characteristics such as colony formation, viability, stem cell marker identification and osteogenic differentiation (using alkaline phosphatase analysis and Alizarin red staining) were comparatively assessed using one-way ANOVA and post hoc Tukey tests using SPSS 13...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#5
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29131300/engineered-scaffolds-and-cell-based-therapy-for-periodontal-regeneration
#6
Daniela Carmagnola, Mihai Tarce, Claudia Dellavia, Lia Rimondini, Elena M Varoni
BACKGROUND: The main objective of regenerative periodontal therapy is to completely restore the periodontal tissues lost. This review summarizes the most recent evidence in support of scaffold- and cell-based tissue engineering, which are expected to play a relevant role in next-generation periodontal regenerative therapy. METHODS: A literature search (PubMed database) was performed to analyze more recently updated articles regarding periodontal regeneration, scaffolds and cell-based technologies...
November 10, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29075537/pulpo-periodontal-regeneration-management-of-partial-failure-revascularization
#7
Said Dhaimy, Sara Dhoum, Hind Amarir, Hafsa El Merini, Sellama Nadifi, Amal El Ouazzani
The aim of this work is to present a case of management of an open apex on a lower molar by using tissue engineering, with two endodontic procedures in the same tooth. We had to resort to pulp regeneration on the distal root and apexification with MTA on the mesial roots after the failure of regenerative therapy on those ones. The management consisted in scheduling regular follow-ups combined with X-rays. After 24 months, the radiological control has shown pulpo-periodontal regeneration associated with walls thickening and distal root elongation and periapical ad integrum healing...
2017: Case Reports in Dentistry
https://www.readbyqxmd.com/read/29069818/inhibiting-phd2-in-bone-marrow-mesenchymal-stem-cells-via-lentiviral-vector-mediated-rna-interference-facilitates-the-repair-of-periodontal-tissue-defects-in-sd-rats
#8
Changxing Chen, Houxuan Li, Jun Jiang, Qian Zhang, Fuhua Yan
Hypoxia-inducible factors (HIFs) play an important role in angiogenesis, and they can activate the expression of several downstream angiogenic factors. HIF-1 is a major transcriptor of HIFs, composed of α and β subunits. Prolyl hydroxylase domain-containing protein 2 (PHD2) is the main catabolic enzyme for HIF-1α, and it can accelerate its degradation under normoxic conditions. PHD2 expression in bone marrow mesenchymal stem cells (BMMSCs) of SD rats was down-regulated under normoxic conditions in this study by utilizing lentiviral vector-mediated RNA interference to promote HIF-1α accumulation, thus enhancing the expression of angiogenic factors...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29045803/chitosan-based-trilayer-scaffold-for-multitissue-periodontal-regeneration
#9
E M Varoni, S Vijayakumar, E Canciani, A Cochis, L De Nardo, G Lodi, L Rimondini, M Cerruti
Periodontal regeneration is still a challenge for periodontists and tissue engineers, as it requires the simultaneous restoration of different tissues-namely, cementum, gingiva, bone, and periodontal ligament (PDL). Here, we synthetized a chitosan (CH)-based trilayer porous scaffold to achieve periodontal regeneration driven by multitissue simultaneous healing. We produced 2 porous compartments for bone and gingiva regeneration by cross-linking with genipin either medium molecular weight (MMW) or low molecular weight (LMW) CH and freeze-drying the resulting scaffolds...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29027282/oestrogen-retains-human-periodontal-ligament-stem-cells-stemness-in-long-term-culture
#10
Qianmin Ou, Xiaoxiao Wang, Yanlan Wang, Yan Wang, Xuefeng Lin
OBJECTIVES: During long-term culture, loss of stemness is observed which greatly restricts the application of human periodontal ligament stem cells (hPDLSCs) in tissue regeneration. Oestrogen (E2) was found to significantly enhance the proliferation and osteogenic differentiation capacity in mesenchymal stem cells. Therefore, in this study, we investigated effects of E2 on hPDLSCs stemness in long-term culture. MATERIALS AND METHODS: Effects of E2 on hPDLSCs stemness were systematically evaluated...
October 12, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29026693/platelet-rich-fibrin-a-paradigm-in-periodontal-therapy-a-systematic-review
#11
REVIEW
Umesh Pratap Verma, Rakesh Kumar Yadav, Manisha Dixit, Abhaya Gupta
Periodontal tissue regeneration has always been a challenge for the periodontists owing to its structural complexity. Although with tissue engineering as a growing multidisciplinary field, this aim has partially been fulfilled. In recent years, platelet-rich fibrin (PRF) has gained wide attention for its utilization as a biocompatible regenerative material not only in dental but also in medical fields. The following systematic review has gathered all the currently available in vitro, animal, and clinical studies utilizing PubMed electronic database from January 2006 to August 2016 highlighting PRF for soft and hard tissue regeneration and/or wound healing...
September 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28975732/a-novel-calcium-accumulating-peptide-gelatin-in-situ-forming-hydrogel-for-enhanced-bone-regeneration
#12
Beom Soo Jo, Yunki Lee, Jin Sook Suh, Yoon Shin Park, Hyun Jung Lee, Jue-Yeon Lee, Jaejin Cho, Gene Lee, Chong Pyoung Chung, Ki Dong Park, Yoon Jeong Park
Bioactive agents, including proteins and peptides, can be loaded into hydrogels to improve bone regenerative capacity with their controlled release. However, the current loading method has focused on physical mixing, which has limited release control. Therefore, alternative conjugation of bioactive agents with hydrogels is highly recommended. Direct chemical conjugation of synthetic peptides containing a functional moiety with a hydrogel would be ideal. Here, we synthesized a bioactive calcium accumulating peptide (CAP) containing a collagen binding motif (CBM), which can induce osteogenic differentiation...
October 4, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28944821/transplantation-of-periodontal-ligament-cell-sheets-expressing-human-%C3%AE-%C3%A2-defensin%C3%A2-3-promotes-anti%C3%A2-inflammation-in-a-canine-model-of-periodontitis
#13
Minwen Zhu, Bo Miao, Jianhua Zhu, Haiyan Wang, Zengtong Zhou
Periodontitis is a chronic oral inflammatory disease caused by microorganisms. Human β‑defensin‑3 (HBD‑3) is an endogenous antimicrobial peptide that inhibits a broad spectrum of microorganisms. Cell sheet technology has been widely applied in tissue and organ reconstructions. In the current study, it was aimed to investigate the anti‑inflammatory effect of periodontal tissue engineered by HBD‑3 gene‑modified periodontal ligament cell (PDLC) sheets, and to identify a suitable method of promoting the regeneration of periodontal tissues...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28860570/cxcl12-overexpression-promotes-the-angiogenesis-potential-of-periodontal-ligament-stem-cells
#14
Lei Zhang, Yong Zhou, Xiaoyu Sun, Jian Zhou, Pishan Yang
Periodontal ligament stem cells (PDLSCs) are a major source of mesenchymal stem cells (MSCs) in adults and are effective for tissue engineering, like promoting angiogenesis and bone regeneration. CXCL12 has been reported to be involved in the recruitment and engraftment of MSCs in wound sites. However, whether CXCL12 potentiates the angiogenesis of PDLSCs is not clear. In this experiment, we transduced PDLSCs with CXCL12, and evaluated the angiogenesis potential of CXCL12-modified PDLSCs through in vitro and in vivo studies...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28859160/igfbp2-enhances-adipogenic-differentiation-potentials-of-mesenchymal-stem-cells-from-wharton-s-jelly-of-the-umbilical-cord-via-jnk-and-akt-signaling-pathways
#15
Yuejun Wang, Yunsong Liu, Zhipeng Fan, Dayong Liu, Fu Wang, Yongsheng Zhou
Mesenchymal stem cell (MSC)-mediated tissue engineering represents a promising strategy to address adipose tissue defects. MSCs derived from Wharton's jelly of the umbilical cord (WJCMSCs) may serve as an ideal source for adipose tissue engineering due to their abundance, safety profile, and accessibility. How to activate the directed differentiation potentials of WJCMSCs is the core point for their clinical applications. A thorough investigation of mechanisms involved in WJCMSC adipogenic differentiation is necessary to support their application in adipose tissue engineering and address shortcomings...
2017: PloS One
https://www.readbyqxmd.com/read/28844309/microbial-modulation-of-stem-cells-and-future-directions-in-regenerative-endodontics
#16
Anibal Diogenes, Kenneth M Hargreaves
Regenerative endodontic procedures (REPs) have been shown to promote the resolution of signs and symptoms of disease and increase survival compared with traditional treatment procedures. However, there is still variable predictability of continued root development and evidence that the tissues formed do not recapitulate the native pulp-dentin complex. There is growing evidence that the apical papilla is capable of surviving prolonged endodontic infection and apical periodontitis and that it represents a rich source of undifferentiated mesenchymal stem cells in REPs...
September 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28844305/regenerative-endodontics-for-adult-patients
#17
Ling He, Sahng G Kim, Qimei Gong, Juan Zhong, Sainan Wang, Xuedong Zhou, Ling Ye, Junqi Ling, Jeremy J Mao
The goal of endodontics is to save teeth. Since inception, endodontic treatments are performed to obturate disinfected root canals with inert materials such as gutta-percha. Although teeth can be saved after successful endodontic treatments, they are devitalized and therefore susceptible to reinfections and fractures. The American Association of Endodontists (AAE) has made a tremendous effort to revitalize disinfected immature permanent teeth in children and adolescents with diagnoses including pulp necrosis or apical periodontitis...
September 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28815884/periodontal-ligament-like-tissue-regeneration-with-drilled-porous-decalcified-dentin%C3%A2-matrix-sheet-composite
#18
Ge Feng, Yan Wu, Yu Yong, Lan Huang, Shu An, Bo Hu, Jun Luo, Jinlin Song
OBJECTIVE: In this study, we constructed a composite by combining the human dental follicle cell sheet and a manual drilled porous decalcified dentin matrix that was used to construct ectopic tissue-engineered periodontal ligament-like tissues in renal capsules of nude mice. MATERIALS AND METHODS: Human dental follicle cells were harvested from human lower third molars and then embedded into a temperature-sensitive culture dish. These cells were then placed into frozen porous decalcified dentin matrix sheets and induced by 50 g/ml ascorbic acid...
August 16, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28805207/periodontal-diseases
#19
REVIEW
Denis F Kinane, Panagiota G Stathopoulou, Panos N Papapanou
Periodontal diseases comprise a wide range of inflammatory conditions that affect the supporting structures of the teeth (the gingiva, bone and periodontal ligament), which could lead to tooth loss and contribute to systemic inflammation. Chronic periodontitis predominantly affects adults, but aggressive periodontitis may occasionally occur in children. Periodontal disease initiation and propagation is through a dysbiosis of the commensal oral microbiota (dental plaque), which then interacts with the immune defences of the host, leading to inflammation and disease...
June 22, 2017: Nature Reviews. Disease Primers
https://www.readbyqxmd.com/read/28795622/animal-models-for-periodontal-tissue-engineering-a-knowledge-generating-process
#20
Karim M Fawzy El-Sayed, Christof E Dörfer
The human periodontium is a uniquely complex vital structure, supporting and anchoring the teeth in their alveolar sockets, thereby playing a decisive role in tooth homeostasis and function. Chronic periodontitis is a highly prevalent immune-inflammatory disease of the periodontium, affecting 15% of adult individuals, and is characterized by progressive destruction of the periodontal tooth-investing tissues, culminating in their irreversible damage. Current periodontal evidence-based treatment strategies achieve periodontal healing via repair processes, mostly combating the inflammatory component of the disease, to halt or reduce prospective periodontal tissue loss...
October 25, 2017: Tissue Engineering. Part C, Methods
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