keyword
MENU ▼
Read by QxMD icon Read
search

periodontal tissue engineering

keyword
https://www.readbyqxmd.com/read/29596323/biofunctionalized-scaffold-in-bone-tissue-repair
#1
Francesca Diomede, Marco D'Aurora, Agnese Gugliandolo, Ilaria Merciaro, Tiziana Orsini, Valentina Gatta, Adriano Piattelli, Oriana Trubiani, Emanuela Mazzon
Bone tissue engineering is based on bone grafting to repair bone defects. Bone graft substitutes can contribute to the addition of mesenchymal stem cells (MSCs) in order to enhance the rate and the quality of defect regeneration. The stem cell secretome contains many growth factors and chemokines, which could affect cellular characteristics and behavior. Conditioned medium (CM) could be used in tissue regeneration avoiding several problems linked to the direct use of MSCs. In this study, we investigated the effect of human periodontal ligament stem cells (hPDLSCs) and their CM on bone regeneration using a commercially available membrane scaffold Evolution (EVO) implanted in rat calvarias...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29565801/mesenchymal-stem-cells-of-dental-origin-for-inducing-tissue-regeneration-in-periodontitis-a-mini-review
#2
REVIEW
Beatriz Hernández-Monjaraz, Edelmiro Santiago-Osorio, Alberto Monroy-García, Edgar Ledesma-Martínez, Víctor Manuel Mendoza-Núñez
Periodontitis is a chronic disease that begins with a period of inflammation of the supporting tissues of the teeth table and then progresses, destroying the tissues until loss of the teeth occurs. The restoration of the damaged dental support apparatus is an extremely complex process due to the regeneration of the cementum, the periodontal ligament, and the alveolar bone. Conventional treatment relies on synthetic materials that fill defects and replace lost dental tissue, but these approaches are not substitutes for a real regeneration of tissue...
March 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29553121/influence-of-diameter-of-fiber-membrane-scaffolds-on-the-biocompatibility-of-hpdl-mesenchymal-stromal-cells
#3
José Luis Suarez-Franco, Febe Carolina Vázquez-Vázquez, Amaury Pozos-Guillen, Juan José Montesinos, Octavio Alvarez-Fregoso, Marco Antonio Alvarez-Perez
This study evaluated the influence in the biocompatibility of human periodontal ligament (hPDL) mesenchymal stromal cell onto poly lactic-acid (PLA) films and PLA fiber membrane. Fiber scaffold was prepared via air jet spinning (AJS) from PLA solutions (6, 7, and 10%) and analyzed using SEM, AFM and FTIR. Biocompatibility was evaluated by adhesion, proliferation and cell-material interaction. PLA film exhibited a smooth and homogenously surface topography in comparison with random orientation of PLA fiber with roughness structure where diameter size depends on PLA solution...
March 17, 2018: Dental Materials Journal
https://www.readbyqxmd.com/read/29527327/dna-methylation-profile-is-associated-with-the-osteogenic-potential-of-three-distinct-human-odontogenic-stem-cells
#4
Tingting Ai, Jieni Zhang, Xuedong Wang, Xiaowen Zheng, Xueyan Qin, Qian Zhang, Weiran Li, Wei Hu, Jiuxiang Lin, Feng Chen
Among the various sources of human autologous stem cells, stem cells isolated from dental tissues exhibit excellent properties in tissue engineering and regenerative medicine. However, the distinct potential of these odontogenic cell lines remains unclear. In this study, we analyzed DNA methylation patterns to determine whether specific differences existed among three different odontogenic cell types. Using the HumanMethylation450 Beadchip, the whole genomes of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and dental follicle progenitor cells (DFPCs) were compared...
2018: Signal Transduction and Targeted Therapy
https://www.readbyqxmd.com/read/29500836/the-effect-of-decellularized-tissue-engineered-constructs-on-periodontal-regeneration
#5
A Farag, S M Hashimi, C Vaquette, P M Bartold, D W Hutmacher, S Ivanovski
AIM: To evaluate the effect of decellularized tissue engineered constructs on cell differentiation in vitro and periodontal regeneration in vivo. MATERIALS AND METHODS: Periodontal ligament cell (PDLC) sheets were loaded on polycaprolactone (PCL) scaffolds then decellularized. Constructs were assessed for their effect on allogenic PDLC and mesenchymal stem cell (MSC) differentiation in vitro, as evaluated by gene expression of bone and periodontal ligament tissue markers post-seeding...
March 3, 2018: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29498025/dental-stem-cells-in-bone-tissue-engineering-current-overview-and-challenges
#6
Pinar Ercal, Gorke Gurel Pekozer, Gamze Torun Kose
The treatment of bone that is impaired due to disease, trauma or tumor resection creates a challenge for both clinicians and researchers. Critical size bone defects are conventionally treated with autografts which are associated with risks such as donor site morbidity and limitations like donor shortage. Bone tissue engineering has become a promising area for the management of critical size bone defects by the employment of biocompatible materials and the discovery of novel stem cell sources. Mesenchymal stem cells (MSCs) can be isolated with ease from various dental tissues including dental pulp stem cells, stem cells from apical papilla, dental follicle stem cells, stem cells from human exfoliated deciduous teeth, periodontal ligament stem cells, gingival stem cells and tooth germ derived stem cells...
March 2, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29470454/mineralization-induction-of-gingival-fibroblasts-and-construction-of-a-sandwich-tissue-engineered-complex-for-repairing-periodontal-defects
#7
Mingxuan Wu, Jie Wang, Yanning Zhang, Huijuan Liu, Fusheng Dong
BACKGROUND The ideal healing technique for periodontal tissue defects would involve the functional regeneration of the alveolar bone, cementum, and periodontal ligament, with new periodontal attachment formation. In this study, gingival fibroblasts were induced and a "sandwich" tissue-engineered complex (a tissue-engineered periodontal membrane between 2 tissue-engineered mineralized membranes) was constructed to repair periodontal defects. We evaluated the effects of gingival fibroblasts used as seed cells on the repair of periodontal defects and periodontal regeneration...
February 22, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29416335/human-%C3%AE-defensin-3-combined-gold-nanoparticles-for-enhancement-of-osteogenic-differentiation-of-human-periodontal-ligament-cells-in-inflammatory-microenvironments
#8
Jing Zhou, Yangheng Zhang, Lingjun Li, Huangmei Fu, Wenrong Yang, Fuhua Yan
Objective: It is a great challenge to absorb and conduct biophysicochemical interactions at the nano-bio interface. Peptides are emerging as versatile materials whose function can be programmed to perform specific tasks. Peptides combined nanoparticles might be utilized as a new approach of treatment. Human β-defensin 3 (hBD3), possesses both antimicrobial and proregeneration properties. Gold nanoparticles (AuNPs) have shown promising applications in the field of tissue engineering. However, the coordinating effects of AuNPs and hBD3 on human periodontal ligament cells (hPDLCs) remain unknown...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29398865/tooth-for-a-tooth-tissue-engineering-made-easy-at-dental-chairside
#9
Vandana Kharidhi Laxman, Rajendra Gururao Desai
Background: Stem cells are unspecialized, multipotent cells which can differentiate into different cell types. The human periodontal ligament encloses subpopulation of Periodontal Ligament Stem Cells (PDLSCs) which are able to regenerate the periodontium by differentiating into various cells of PDL. Literature have shown the promising results of periodontal regeneration by exvivo cultured transplantation of PDLSCs into the osseous defects. Methods: Based on current literature on use of ex vivo culture and associated problems, a novel technique is being presented , Stem cell application in periodontal regeneration (SAI-PRT) which uses autologous PDLSCs along with its niche adhering to extracted tooth root for direct application using gelatin sponge as scaffold in regeneration of intrabony periodontal defect bypassing ex-vivo culture...
March 2017: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29368432/study-of-platelet-rich-fibrin-combined-with-rat-periodontal-ligament-stem-cells-in-periodontal-tissue-regeneration
#10
Xuejing Duan, Zhiyong Lin, Xiujuan Lin, Zhiqiang Wang, Yihua Wu, Mei Ji, Wei Lu, Xiaoyang Wang, Dongsheng Zhang
The objective of this study was to investigate the advantages and feasibility of periodontal tissue regeneration using platelet-rich fibrin (PRF) combined with rat periodontal ligament stem cells (PDLSCs) for the first time. We first determined the effect of PRF on rat PDLSCs in vitro. We next conducted an in vivo study, in which a tissue engineering technique was performed to repair periodontal defects in five groups: a blank group, collagen group (implanted collagen membrane), collagen + cells group (implanted collagen membrane and rat PDLSCs), PRF group (implanted PRF membrane) and PRF + cells group (implanted PRF membrane and rat PDLSCs)...
February 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29365250/proteolytic-nanoparticles-replace-a-surgical-blade-by-controllably-remodeling-the-oral-connective-tissue
#11
Assaf Zinger, Omer Adir, Matan Alper, Assaf Simon, Maria Poley, Chen Tzror, Zvi Yaari, Majd Krayem, Shira Kasten, Guy Nawy, Avishai Herman, Yael Nir, Sharon Akrish, Tidhar Klein, Janna Shainsky-Roitman, Dov Hershkovitz, Avi Schroeder
Surgical blades are common medical tools. However, blades cannot distinguish between healthy and diseased tissue, thereby creating unnecessary damage, lengthening recovery and increasing pain. We propose that surgical procedures can rely on natural tissue remodeling tools - enzymes, which are the same tools our body uses to repair itself. Through a combination of nanotechnology and a controllably activated proteolytic enzyme we performed a targeted surgical task in the oral cavity. More specifically, we engineered nanoparticles that contain collagenase in a deactivated form...
January 24, 2018: ACS Nano
https://www.readbyqxmd.com/read/29360752/mesenchymal-stem-cells-derived-from-healthy-and-diseased-human-gingiva-support-osteogenesis-on-electrospun-polycaprolactone-scaffolds
#12
Catherine Jauregui, Suyog Yoganarasimha, Parthasarathy Madurantakam
Periodontitis is a chronic inflammatory disease affecting almost half of the adult US population. Gingiva is an integral part of the periodontium and has recently been identified as a source of adult gingiva-derived mesenchymal stem cells (GMSCs). Given the prevalence of periodontitis, the purpose of this study is to evaluate differences between GMSCs derived from healthy and diseased gingival tissues and explore their potential in bone engineering. Primary clonal cell lines were established from harvested healthy and diseased gingival and characterized for expression of known stem-cell markers and multi-lineage differentiation potential...
January 23, 2018: Bioengineering
https://www.readbyqxmd.com/read/29359787/the-differential-effect-of-basic-fibroblast-growth-factor-and-stromal-cell%C3%A2-derived-factor%C3%A2-1-pretreatment-on-bone-morrow-mesenchymal-stem-cells-osteogenic-differentiation-potency
#13
Ruolin Wang, Wenhua Liu, Mi Du, Chengzhe Yang, Xuefen Li, Pishan Yang
In situ tissue engineering has become a novel strategy to repair periodontal/bone tissue defects. The choice of cytokines that promote the recruitment and proliferation, and potentiate and maintain the osteogenic differentiation ability of mesenchymal stem cells (MSCs) is the key point in this technique. Stromal cell‑derived factor‑1 (SDF‑1) and basic fibroblast growth factor (bFGF) have the ability to promote the recruitment, and proliferation of MSCs; however, the differential effect of SDF‑1 and bFGF pretreatment on MSC osteogenic differentiation potency remains to be explored...
December 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29356251/dedifferentiated-fat-dfat-cells-a-cell-source-for-oral-and-maxillofacial-tissue-engineering
#14
Naotaka Kishimoto, Yoshitomo Honda, Yoshihiro Momota, Simon D Tran
Tissue engineering is a promising method for the regeneration of oral and maxillofacial tissues. Proper selection of a cell source is important for the desired application. This review describes the discovery and usefulness of Dedifferentiated Fat (DFAT) cells as a cell source for tissue engineering. DFAT cells are a highly homogeneous cell population (high purity), highly proliferative, and possess a multilineage potential for differentiation into various cell types under proper in vitro inducing conditions and in vivo...
January 21, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29274111/influence-of-ciprofloxacin-based-additives-on-the-hydrolysis-of-nanofiber-polyurethane-membranes
#15
Meghan E E Wright, Andrew T Wong, Daniel Levitt, Ian C Parrag, Meilin Yang, J Paul Santerre
A degradable polycarbonate urethane (PCNU) and an antimicrobial oligomer (AO) were used to generate anti-infective nanofiber scaffolds through blend electrospinning. The AO consists of two molecules of ciprofloxacin (CF) bound through hydrolysable linkages to triethylene glycol. The membranes were conceived for use as tissue engineering scaffolds for the regeneration of soft tissues for the periodontium, where there would be a need for a local dose of antibiotic to the periodontal space as the scaffold degrades in order to prevent biomaterial-associated infection...
May 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29259720/cell-transfer-technology-for-tissue-engineering
#16
REVIEW
Keiko Akazawa, Kengo Iwasaki, Mizuki Nagata, Naoki Yokoyama, Hirohito Ayame, Kazumasa Yamaki, Yuichi Tanaka, Izumi Honda, Chikako Morioka, Tsuyoshi Kimura, Motohiro Komaki, Akio Kishida, Yuichi Izumi, Ikuo Morita
We recently developed novel cell transplantation method "cell transfer technology" utilizing photolithography. Using this method, we can transfer ex vivo expanded cells onto scaffold material in desired patterns, like printing of pictures and letters on a paper. We have investigated the possibility of this novel method for cell-based therapy using several disease models. We first transferred endothelial cells in capillary-like patterns on amnion. The transplantation of the endothelial cell-transferred amnion enhanced the reperfusion in mouse ischemic limb model...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#17
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29213288/microrna-214-suppresses-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-by-targeting-atf4
#18
Siqi Yao, Wei Zhao, Qianmin Ou, Lanchen Liang, Xuefeng Lin, Yan Wang
Periodontitis is the main cause of adult tooth loss. Stem cell-based tissue engineering has become a promising therapy for periodontitis treatment. To date, human periodontal ligament stem cells (hPDLSCs) have been shown to be a favorable source for tissue engineering, but modulatory mechanisms of hPDLSCs remain unclear. Approximately 60% of mammalian genes are the targets of over 2000 miRNAs in multiple human cell types, and miRNAs are able to influence various biological processes in the human body, including bone formation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29201597/current-concepts-of-epigenetics-and-its-role-in-periodontitis
#19
REVIEW
Lena Larsson
Purpose: The focus of this review is to provide an overview of the recent findings on the role of epigenetic mechanisms in periodontal disease, including disease susceptibility, progression, and as potential treatment options. Recent Findings: The findings on the influence of oral pathogens on epigenetic regulation of pathogen recognition receptors, such as Toll-like receptors, as well as pro-inflammatory cytokines suggest an important role for epigenetics in the regulation of the host immune response...
2017: Current Oral Health Reports
https://www.readbyqxmd.com/read/29184628/comparison-of-in-vitro-properties-of-periodontal-ligament-stem-cells-derived-from-permanent-and-deciduous-teeth
#20
Masoumeh Khoshhal, Iraj Amiri, Leila Gholami
Background. Stem cells have contributed to the development of tissue-engineered-based regenerative periodontal therapies. In order to find the best stem cell sources for such therapies, the biologic properties of stem cells isolated from periodontal ligaments (PDL) of deciduous (DePDLSC) and permanent (PePDLSC) teeth were comparatively evaluated. Methods. PDL stem cells were isolated from six sound fully erupted premolars and six deciduous canines of healthy subjects. In vitro biologic characteristics such as colony formation, viability, stem cell marker identification and osteogenic differentiation (using alkaline phosphatase analysis and Alizarin red staining) were comparatively assessed using one-way ANOVA and post hoc Tukey tests using SPSS 13...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
keyword
keyword
42689
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"