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periodontal tissue engineering

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https://www.readbyqxmd.com/read/29416335/human-%C3%AE-defensin-3-combined-gold-nanoparticles-for-enhancement-of-osteogenic-differentiation-of-human-periodontal-ligament-cells-in-inflammatory-microenvironments
#1
Jing Zhou, Yangheng Zhang, Lingjun Li, Huangmei Fu, Wenrong Yang, Fuhua Yan
Objective: It is a great challenge to absorb and conduct biophysicochemical interactions at the nano-bio interface. Peptides are emerging as versatile materials whose function can be programmed to perform specific tasks. Peptides combined nanoparticles might be utilized as a new approach of treatment. Human β-defensin 3 (hBD3), possesses both antimicrobial and proregeneration properties. Gold nanoparticles (AuNPs) have shown promising applications in the field of tissue engineering. However, the coordinating effects of AuNPs and hBD3 on human periodontal ligament cells (hPDLCs) remain unknown...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29398865/tooth-for-a-tooth-tissue-engineering-made-easy-at-dental-chairside
#2
Vandana Kharidhi Laxman, Rajendra Gururao Desai
Background: Stem cells are unspecialized, multipotent cells which can differentiate into different cell types. The human periodontal ligament encloses subpopulation of Periodontal Ligament Stem Cells (PDLSCs) which are able to regenerate the periodontium by differentiating into various cells of PDL. Literature have shown the promising results of periodontal regeneration by exvivo cultured transplantation of PDLSCs into the osseous defects. Methods: Based on current literature on use of ex vivo culture and associated problems, a novel technique is being presented , Stem cell application in periodontal regeneration (SAI-PRT) which uses autologous PDLSCs along with its niche adhering to extracted tooth root for direct application using gelatin sponge as scaffold in regeneration of intrabony periodontal defect bypassing ex-vivo culture...
March 2017: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29368432/study-of-platelet-rich-fibrin-combined-with-rat-periodontal-ligament-stem-cells-in-periodontal-tissue-regeneration
#3
Xuejing Duan, Zhiyong Lin, Xiujuan Lin, Zhiqiang Wang, Yihua Wu, Mei Ji, Wei Lu, Xiaoyang Wang, Dongsheng Zhang
The objective of this study was to investigate the advantages and feasibility of periodontal tissue regeneration using platelet-rich fibrin (PRF) combined with rat periodontal ligament stem cells (PDLSCs) for the first time. We first determined the effect of PRF on rat PDLSCs in vitro. We next conducted an in vivo study, in which a tissue engineering technique was performed to repair periodontal defects in five groups: a blank group, collagen group (implanted collagen membrane), collagen + cells group (implanted collagen membrane and rat PDLSCs), PRF group (implanted PRF membrane) and PRF + cells group (implanted PRF membrane and rat PDLSCs)...
February 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29365250/proteolytic-nanoparticles-replace-a-surgical-blade-by-controllably-remodeling-the-oral-connective-tissue
#4
Assaf Zinger, Omer Adir, Matan Alper, Assaf Simon, Maria Poley, Chen Tzror, Zvi Yaari, Majd Krayem, Shira Kasten, Guy Nawy, Avishai Herman, Yael Nir, Sharon Akrish, Tidhar Klein, Janna Shainsky-Roitman, Dov Hershkovitz, Avi Schroeder
Surgical blades are common medical tools. However, blades cannot distinguish between healthy and diseased tissue, thereby creating unnecessary damage, lengthening recovery and increasing pain. We propose that surgical procedures can rely on natural tissue remodeling tools - enzymes, which are the same tools our body uses to repair itself. Through a combination of nanotechnology and a controllably activated proteolytic enzyme we performed a targeted surgical task in the oral cavity. More specifically, we engineered nanoparticles that contain collagenase in a deactivated form...
January 24, 2018: ACS Nano
https://www.readbyqxmd.com/read/29360752/mesenchymal-stem-cells-derived-from-healthy-and-diseased-human-gingiva-support-osteogenesis-on-electrospun-polycaprolactone-scaffolds
#5
Catherine Jauregui, Suyog Yoganarasimha, Parthasarathy Madurantakam
Periodontitis is a chronic inflammatory disease affecting almost half of the adult US population. Gingiva is an integral part of the periodontium and has recently been identified as a source of adult gingiva-derived mesenchymal stem cells (GMSCs). Given the prevalence of periodontitis, the purpose of this study is to evaluate differences between GMSCs derived from healthy and diseased gingival tissues and explore their potential in bone engineering. Primary clonal cell lines were established from harvested healthy and diseased gingival and characterized for expression of known stem-cell markers and multi-lineage differentiation potential...
January 23, 2018: Bioengineering
https://www.readbyqxmd.com/read/29359787/the-differential-effect-of-basic-fibroblast-growth-factor-and-stromal-cell%C3%A2-derived-factor%C3%A2-1-pretreatment-on-bone-morrow-mesenchymal-stem-cells-osteogenic-differentiation-potency
#6
Ruolin Wang, Wenhua Liu, Mi Du, Chengzhe Yang, Xuefen Li, Pishan Yang
In situ tissue engineering has become a novel strategy to repair periodontal/bone tissue defects. The choice of cytokines that promote the recruitment and proliferation, and potentiate and maintain the osteogenic differentiation ability of mesenchymal stem cells (MSCs) is the key point in this technique. Stromal cell‑derived factor‑1 (SDF‑1) and basic fibroblast growth factor (bFGF) have the ability to promote the recruitment, and proliferation of MSCs; however, the differential effect of SDF‑1 and bFGF pretreatment on MSC osteogenic differentiation potency remains to be explored...
December 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29356251/dedifferentiated-fat-dfat-cells-a-cell-source-for-oral-and-maxillofacial-tissue-engineering
#7
Naotaka Kishimoto, Yoshitomo Honda, Yoshihiro Momota, Simon D Tran
Tissue engineering is a promising method for the regeneration of oral and maxillofacial tissues. Proper selection of a cell source is important for the desired application. This review describes the discovery and usefulness of Dedifferentiated Fat (DFAT) cells as a cell source for tissue engineering. DFAT cells are a highly homogeneous cell population (high purity), highly proliferative, and possess a multilineage potential for differentiation into various cell types under proper in vitro inducing conditions and in vivo...
January 21, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29274111/influence-of-ciprofloxacin-based-additives-on-the-hydrolysis-of-nanofiber-polyurethane-membranes
#8
Meghan Ee Wright, Andrew T Wong, Daniel Levitt, Ian C Parrag, Meilin Yang, J Paul Santerre
A degradable polycarbonate urethane (PCNU) and an antimicrobial oligomer (AO) were used to generate anti-infective nanofiber scaffolds via blend electrospinning. The AO consists of two molecules of ciprofloxacin (CF) bound via hydrolysable linkages to triethylene glycol (TEG). The membranes were conceived for use as tissue engineering scaffolds for the regeneration of soft tissues for the periodontium, where there would be a need for a local dose of antibiotic to the periodontal space as the scaffold degrades in order to prevent biomaterial-associated infection...
December 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29259720/cell-transfer-technology-for-tissue-engineering
#9
REVIEW
Keiko Akazawa, Kengo Iwasaki, Mizuki Nagata, Naoki Yokoyama, Hirohito Ayame, Kazumasa Yamaki, Yuichi Tanaka, Izumi Honda, Chikako Morioka, Tsuyoshi Kimura, Motohiro Komaki, Akio Kishida, Yuichi Izumi, Ikuo Morita
We recently developed novel cell transplantation method "cell transfer technology" utilizing photolithography. Using this method, we can transfer ex vivo expanded cells onto scaffold material in desired patterns, like printing of pictures and letters on a paper. We have investigated the possibility of this novel method for cell-based therapy using several disease models. We first transferred endothelial cells in capillary-like patterns on amnion. The transplantation of the endothelial cell-transferred amnion enhanced the reperfusion in mouse ischemic limb model...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#10
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29213288/microrna-214-suppresses-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-by-targeting-atf4
#11
Siqi Yao, Wei Zhao, Qianmin Ou, Lanchen Liang, Xuefeng Lin, Yan Wang
Periodontitis is the main cause of adult tooth loss. Stem cell-based tissue engineering has become a promising therapy for periodontitis treatment. To date, human periodontal ligament stem cells (hPDLSCs) have been shown to be a favorable source for tissue engineering, but modulatory mechanisms of hPDLSCs remain unclear. Approximately 60% of mammalian genes are the targets of over 2000 miRNAs in multiple human cell types, and miRNAs are able to influence various biological processes in the human body, including bone formation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29201597/current-concepts-of-epigenetics-and-its-role-in-periodontitis
#12
REVIEW
Lena Larsson
Purpose: The focus of this review is to provide an overview of the recent findings on the role of epigenetic mechanisms in periodontal disease, including disease susceptibility, progression, and as potential treatment options. Recent Findings: The findings on the influence of oral pathogens on epigenetic regulation of pathogen recognition receptors, such as Toll-like receptors, as well as pro-inflammatory cytokines suggest an important role for epigenetics in the regulation of the host immune response...
2017: Current Oral Health Reports
https://www.readbyqxmd.com/read/29184628/comparison-of-in-vitro-properties-of-periodontal-ligament-stem-cells-derived-from-permanent-and-deciduous-teeth
#13
Masoumeh Khoshhal, Iraj Amiri, Leila Gholami
Background. Stem cells have contributed to the development of tissue-engineered-based regenerative periodontal therapies. In order to find the best stem cell sources for such therapies, the biologic properties of stem cells isolated from periodontal ligaments (PDL) of deciduous (DePDLSC) and permanent (PePDLSC) teeth were comparatively evaluated. Methods. PDL stem cells were isolated from six sound fully erupted premolars and six deciduous canines of healthy subjects. In vitro biologic characteristics such as colony formation, viability, stem cell marker identification and osteogenic differentiation (using alkaline phosphatase analysis and Alizarin red staining) were comparatively assessed using one-way ANOVA and post hoc Tukey tests using SPSS 13...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#14
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29131300/engineered-scaffolds-and-cell-based-therapy-for-periodontal-regeneration
#15
Daniela Carmagnola, Mihai Tarce, Claudia Dellavia, Lia Rimondini, Elena M Varoni
BACKGROUND: The main objective of regenerative periodontal therapy is to completely restore the periodontal tissues lost. This review summarizes the most recent evidence in support of scaffold- and cell-based tissue engineering, which are expected to play a relevant role in next-generation periodontal regenerative therapy. METHODS: A literature search (PubMed database) was performed to analyze more recently updated articles regarding periodontal regeneration, scaffolds and cell-based technologies...
November 10, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29075537/pulpo-periodontal-regeneration-management-of-partial-failure-revascularization
#16
Said Dhaimy, Sara Dhoum, Hind Amarir, Hafsa El Merini, Sellama Nadifi, Amal El Ouazzani
The aim of this work is to present a case of management of an open apex on a lower molar by using tissue engineering, with two endodontic procedures in the same tooth. We had to resort to pulp regeneration on the distal root and apexification with MTA on the mesial roots after the failure of regenerative therapy on those ones. The management consisted in scheduling regular follow-ups combined with X-rays. After 24 months, the radiological control has shown pulpo-periodontal regeneration associated with walls thickening and distal root elongation and periapical ad integrum healing...
2017: Case Reports in Dentistry
https://www.readbyqxmd.com/read/29069818/inhibiting-phd2-in-bone-marrow-mesenchymal-stem-cells-via-lentiviral-vector-mediated-rna-interference-facilitates-the-repair-of-periodontal-tissue-defects-in-sd-rats
#17
Changxing Chen, Houxuan Li, Jun Jiang, Qian Zhang, Fuhua Yan
Hypoxia-inducible factors (HIFs) play an important role in angiogenesis, and they can activate the expression of several downstream angiogenic factors. HIF-1 is a major transcriptor of HIFs, composed of α and β subunits. Prolyl hydroxylase domain-containing protein 2 (PHD2) is the main catabolic enzyme for HIF-1α, and it can accelerate its degradation under normoxic conditions. PHD2 expression in bone marrow mesenchymal stem cells (BMMSCs) of SD rats was down-regulated under normoxic conditions in this study by utilizing lentiviral vector-mediated RNA interference to promote HIF-1α accumulation, thus enhancing the expression of angiogenic factors...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29045803/chitosan-based-trilayer-scaffold-for-multitissue-periodontal-regeneration
#18
E M Varoni, S Vijayakumar, E Canciani, A Cochis, L De Nardo, G Lodi, L Rimondini, M Cerruti
Periodontal regeneration is still a challenge for periodontists and tissue engineers, as it requires the simultaneous restoration of different tissues-namely, cementum, gingiva, bone, and periodontal ligament (PDL). Here, we synthetized a chitosan (CH)-based trilayer porous scaffold to achieve periodontal regeneration driven by multitissue simultaneous healing. We produced 2 porous compartments for bone and gingiva regeneration by cross-linking with genipin either medium molecular weight (MMW) or low molecular weight (LMW) CH and freeze-drying the resulting scaffolds...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29027282/oestrogen-retains-human-periodontal-ligament-stem-cells-stemness-in-long-term-culture
#19
Qianmin Ou, Xiaoxiao Wang, Yanlan Wang, Yan Wang, Xuefeng Lin
OBJECTIVES: During long-term culture, loss of stemness is observed which greatly restricts the application of human periodontal ligament stem cells (hPDLSCs) in tissue regeneration. Oestrogen (E2) was found to significantly enhance the proliferation and osteogenic differentiation capacity in mesenchymal stem cells. Therefore, in this study, we investigated effects of E2 on hPDLSCs stemness in long-term culture. MATERIALS AND METHODS: Effects of E2 on hPDLSCs stemness were systematically evaluated...
October 12, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29026693/platelet-rich-fibrin-a-paradigm-in-periodontal-therapy-a-systematic-review
#20
REVIEW
Umesh Pratap Verma, Rakesh Kumar Yadav, Manisha Dixit, Abhaya Gupta
Periodontal tissue regeneration has always been a challenge for the periodontists owing to its structural complexity. Although with tissue engineering as a growing multidisciplinary field, this aim has partially been fulfilled. In recent years, platelet-rich fibrin (PRF) has gained wide attention for its utilization as a biocompatible regenerative material not only in dental but also in medical fields. The following systematic review has gathered all the currently available in vitro, animal, and clinical studies utilizing PubMed electronic database from January 2006 to August 2016 highlighting PRF for soft and hard tissue regeneration and/or wound healing...
September 2017: Journal of International Society of Preventive & Community Dentistry
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