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https://www.readbyqxmd.com/read/29697396/influence-of-tricalcium-silicate-on-course-of-traumatic-pulpitis
#1
I Kovach, K Buniatian, A Makarevych, A Verbyts'ka, V Gargin
The use of Tricalcium Silicate (TS) as an odontotropic preparation makes it possible to create a hermetic crown restoration with a high degree of adhesion. However, the use of TS silicate by direct pulp capping remains disputable. The aim of this study was to determine the effects of TS on course of traumatic pulpitis by detection of morpho-functional peculiarities of changes in pulp tissue. We performed experimental investigation (on rabbits, males, aging three-month) for study of the morpho-functional changes of the pulp tissues with modeling of traumatic pulpitis and direct pulp capping with TS preparation (8 animals, investigated group) and calcium hydroxide (Calasept, NORDISKA DENTAL) preparation (8 animals, comparison group)...
March 2018: Georgian Medical News
https://www.readbyqxmd.com/read/29679496/detection-of-bone-marrow-derived-fibrocytes-in-human-dental-pulp-repair
#2
N Yoshiba, N Edanami, A Tohma, R Takeuchi, N Ohkura, A Hosoya, Y Noiri, H Nakamura, K Yoshiba
AIM: To explore the expression profile of CD45+/pro-collagen I+ fibrocytes in intact dental pulps as well as during wound healing in adult dental pulp tissue. METHODOLOGY: A total of 16 healthy permanent teeth were obtained from young patients (18 to 25 years) undergoing orthodontic treatment. Routine pulp capping with mineral trioxide aggregate (MTA) was performed under local anaesthesia to induce a mineralized barrier at the exposed surface. Teeth were extracted from patients after 7, 14, and 35 days...
April 21, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29660488/changes-in-the-radicular-pulp-dentine-complex-in-healthy-intact-teeth-and-in-response-to-deep-caries-or-restorations-a-histological-and-histobacteriological-study
#3
Domenico Ricucci, Simona Loghin, Li-Na Niu, Franklin R Tay
INTRODUCTION: The present study reported the histological events that occurred in the radicular pulp of human mature teeth in the presence of medium/deep untreated caries lesions, and those teeth with restorations or direct pulp capping, with particular emphasis on the morphology of the canal wall dentine and the odontoblast layer. METHODS: Sixty-two teeth with medium/deep caries lesions, extensive restorations or after application of a direct pulp capping procedure were obtained from 57 subjects...
April 13, 2018: Journal of Dentistry
https://www.readbyqxmd.com/read/29575555/in-vitro-and-in-vivo-effects-of-a-novel-bioactive-glass-based-cement-used-as-a-direct-pulp-capping-agent
#4
Kaori Hanada, Takahiko Morotomi, Ayako Washio, Naomi Yada, Kou Matsuo, Hiroki Teshima, Kazuyoshi Yokota, Chiaki Kitamura
Direct pulp capping is an important procedure for preserving pulp viability. The pulp capping agent must possess several properties, including usability, biocompatibility, and the ability to induce reparative dentin formation. In this study, a novel bioactive glass-based cement was examined to determine whether the cement has the necessary properties to act as a direct pulp capping agent. Physicochemical properties of the bioactive glass-based cement and in vitro effects of the cement on odontoblast-like cells, as well as in vivo effects on the exposed dental pulp, were analyzed...
March 25, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29483723/-influence-of-setting-time-on-bond-strength-of-different-bioactive-pulp-capping-materials-with-dental-adhesive
#5
Y T Dong, F C Tian, B Jia, B Zu, X Y Wang
OBJECTIVE: To investigate influence of setting time on bond strength of different bioactive pulp capping materials with self-etch or etch-and-rinse adhesive. METHODS: Sixty specimens were prepared for each of the three tested capping materials, namely mineral trioxide aggregate (MTA), iRoot BP Plus (BP) and iRoot FS (FS). Specimens of each material were divided into three groups and bonded at three setting time points of the materials respectively: initial setting time (4 h for MTA, 2 h for BP and 20 min for FS), 24 h after application and 7 d after application...
February 18, 2018: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29467418/exogenous-nitric-oxide-stimulates-the-odontogenic-differentiation-of-rat-dental-pulp-stem-cells
#6
Soichiro Sonoda, Yu-Feng Mei, Ikiru Atsuta, Atsushi Danjo, Haruyoshi Yamaza, Shion Hama, Kento Nishida, Ronghao Tang, Yukari Kyumoto-Nakamura, Norihisa Uehara, Toshio Kukita, Fusanori Nishimura, Takayoshi Yamaza
Nitric oxide (NO) is thought to play a pivotal regulatory role in dental pulp tissues under both physiological and pathological conditions. However, little is known about the NO functions in dental pulp stem cells (DPSCs). We examined the direct actions of a spontaneous NO gas-releasing donor, NOC-18, on the odontogenic capacity of rat DPSCs (rDPSCs). In the presence of NOC-18, rDPSCs were transformed into odontoblast-like cells with long cytoplasmic processes and a polarized nucleus. NOC-18 treatment increased alkaline phosphatase activity and enhanced dentin-like mineralized tissue formation and the expression levels of several odontoblast-specific genes, such as runt related factor 2, dentin matrix protein 1 and dentin sialophosphoprotein, in rDPSCs...
February 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29405371/disclosing-the-physiology-of-pulp-tissue-for-vital-pulp-therapy
#7
REVIEW
W L O da Rosa, E Piva, A F da Silva
The discovery that dentine is a reservoir of bioactive molecules that can be recruited on demand has attracted efforts to develop new protocols and materials for vital pulp therapy (VPT). The noncollagenous proteins (NCPs) present in the dentine extracellular matrix (ECM) include growth factors (TGF-β1, BMP-7, FGF-2, IGF-1 and IGF-2, NGF and GDNF), extracellular matrix molecules (DSP, DPP, BSP, DMP-1 and DSPP) and both anti-inflammatory and pro-inflammatory chemokines and cytokines (TNF-α, IL-1, IL-6 and IL-10)...
February 6, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29279572/reactions-of-human-dental-pulp-cells-to-capping-agents-in-the-presence-or-absence-of-bacterial-exposure
#8
Shiwei Cai, Wenjian Zhang, Gena Tribble, Wei Chen
An ideal pulp-capping agent needs to have good biocompatibility and promote reparative dentinogenesis. Although the effects of capping agents on healthy pulp are known, limited data regarding their effects on bacterial contaminated pulp are available. This study aimed to evaluate the reaction of contaminated pulps to various capping agents to assist clinicians in making informed decisions. Human dental pulp (HDP) cell cultures were developed from extracted human molars. The cells were exposed to a bacterial cocktail comprising Porphyromonas gingivalis, Prevotella intermedia, and Streptococcus gordonii before being cocultured with capping agents such as mineral trioxide aggregate (MTA) Portland cement (PC), and Dycal...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29260758/-regeneration-of-dental-pulp-tissue-using-pulpal-autologous-mesenchymal-stem-cells-and-platelet-rich-plasma
#9
A A Kulakov, D V Goldshtein, E K Krechina, T B Bukharova, A V Volkov, A K Gadzhiev
Regeneration of pulp and dentin could be important in operative dentistry as a method to save teeth. Currently cell population from dental pulp of deciduous and permanent teeth of humans and laboratory animals are isolated and characterized. The paper presents a study on pulp regeneration using autologous mesenchymal stromal cells from pulp of molars in combination with fibrin clot, transplanted in pulp chamber of miniature pigs after pulp removal. The results proved that transplantation of autologous multipotent stromal cells of dental pulp in combination with autologous platelet-rich plasma in pulp chamber of miniature pigs after pulp removal leads to pulp restoration and reparative dentinogenesis with dentinal bridge formation on the 30th day...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29260756/-optimization-of-reparative-dentinogenesis-in-experimental-osteoporosis
#10
N I Bykova, S V Sirak, T L Kobylkina, A V Odolsky, I M Bykov, A V Arutyunov
The aim of the study was to assess histochemical changes of the dental pulp in direct pulp capping/experimental osteoporosis animal model. The study was performed on 20 two-year sheep with simulated acute pulpitis divided in 2 groups: main (15 animals/120 teeth) and control (5 animals/40 teeth). Direct pulp capping in the main group included tissue-engineered structure composed of a hydrogel PuraMatrix/3DM with ectomesenchymal stem cells immobilized on collagen sponge. In the control group collagen sponges with hydrocortisone furatsilin, chondroitin sulfate, аnaesthesinum were used for the same purpose...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29225635/-in-vitro-comparison-of-cytotoxicity-and-genotoxicity-of-three-vital-pulp-capping-materials
#11
Azadeh Zakerzadeh, Ehsan Esnaashari, Sonia Dadfar
Introduction: Direct pulp capping (DPC) is a treatment for maintaining pulp vitality and its biological function. Ideally, pulp capping agents are expected to induce pulp cells to form hard tissue. This in vitro study assessed the cytotoxicity and genotoxicity of three vital pulp capping (VPC) agents naming Biodentine (Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggregate (ProRoot MTA; Dentsply, Tulsa Dental, Tulsa, OK, USA) and TheraCal LC (Bisco Inc, Schamburg, IL, USA) on human dental pulp fibroblasts...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/29179973/mechanism-of-bioactive-molecular-extraction-from-mineralized-dentin-by-calcium-hydroxide-and-tricalcium-silicate-cement
#12
Xue-Qing Huang, John Camba, Li-Sha Gu, Brian E Bergeron, Domenico Ricucci, David H Pashley, Franklin R Tay, Li-Na Niu
OBJECTIVES: The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH)2 ) and tricalcium silicate cements (TSC). METHODS AND RESULTS: Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH)2 or a set, hydrated TSC for 1-3 months. A one micron thick collagen degradation zone was observed on the dentin surface...
February 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29155305/hey1-and-hey2-are-differently-expressed-during-mouse-tooth-development
#13
Kotono Kibe, Mitsushiro Nakatomi, Shinji Kataoka, Takashi Toyono, Yuji Seta
The Hey family (also known as Chf, Herp, Hesr, and Hrt) is a set of Hairy/Enhancer of Split-related basic helix-loop-helix type transcription factors. Hey1, Hey2, and HeyL have been identified in mammals. Although Hey proteins are known to regulate cardiovascular development, muscle homeostasis, osteogenesis, neurogenesis, and oncogenesis, their roles in tooth development have been largely obscure. Therefore, this study aimed to clarify detailed spatiotemporal expression patterns of Hey1 and Hey2 in developing molars and incisors of mice by section in situ hybridization...
January 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29079052/biocompatibility-of-new-pulp-capping-materials-neomta-plus-mta-repair-hp-and-biodentine-on-human-dental-pulp-stem-cells
#14
Christopher J Tomás-Catalá, Mar Collado-González, David García-Bernal, Ricardo E Oñate-Sánchez, Leopoldo Forner, Carmen Llena, Adrián Lozano, José M Moraleda, Francisco J Rodríguez-Lozano
INTRODUCTION: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, NeoMTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). METHODS: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy...
January 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29042078/bioactivity-and-fluoride-release-of-strontium-and-fluoride-modified-biodentine
#15
Hazel O Simila, Natalia Karpukhina, Robert G Hill
Biodentine™ is a novel tricalcium silicate based material used both as a coronal dentine replacement and in pulp therapy. Its multiple use in sealing perforations, pulp capping and as a temporary restoration arises from its ability to promote dentine formation and to confer an excellent marginal seal. However, there is still room for improvement of this cement as it lacks the anticariogenic effect typically conferred by fluoride ion release as seen in glass ionomer cement based dental materials. Therefore, this study was conducted to investigate the impact of bioactive glass addition to Biodentine™...
January 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29032813/light-cured-tricalcium-silicate-toxicity-to-the-dental-pulp
#16
Charlotte Jeanneau, Patrick Laurent, Charlotte Rombouts, Thomas Giraud, Imad About
INTRODUCTION: Numerous studies reported dentin bridge formation after pulp capping with tricalcium silicates. By contrast, pulp capping with resins leads to pulp toxicity and inflammation. Hybrid materials made up of tricalcium silicates and resins have also been developed to be used in direct pulp capping. This work was designed to study the consequences of adding resins to tricalcium silicates by investigating TheraCal (BISCO, Lançon De Provence, France) and Biodentine (Septodont, Saint Maur des Fosses, France) interactions with the dental pulp...
October 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28952538/characterization-of-chlorhexidine-loaded-calcium-hydroxide-microparticles-as-a-potential-dental-pulp-capping-material
#17
Balasankar M Priyadarshini, Subramanian T Selvan, Karthikeyan Narayanan, Amr S Fawzy
This study explores the delivery of novel calcium hydroxide [Ca(OH)₂] microparticles loaded with chlorhexidine (CHX) for potential dental therapeutic and preventive applications. Herein, we introduce a new approach for drug-delivery to deep dentin-surfaces in the form of drug-loaded microparticles. Unloaded Ca(OH)₂ [Ca(OH)₂/Blank] and CHX-loaded/Ca(OH)₂ microparticles were fabricated by aqueous chemical-precipitation technique. The synthesized-microparticles were characterized in vitro for determination of surface-morphology, crystalline-features and thermal-properties examined by energy-dispersive X-ray scanning and transmission electron-microscopy (EDX-SEM/TEM), Fourier-transform infrared-spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and differential scanning-calorimetry (DSC)...
June 22, 2017: Bioengineering
https://www.readbyqxmd.com/read/28866191/nanosized-cap-silk-fibroin-pcl-peg-pcl-pcl-based-bilayer-membranes-for-guided-bone-regeneration
#18
Sibel Türkkan, A Engin Pazarçeviren, Dilek Keskin, Nesrin E Machin, Özgür Duygulu, Ayşen Tezcaner
Guided bone regeneration (GBR) concept has been developed to prevent the formation of non-functional scar tissue layer on defect site by undertaking barrier role. In this study, a new bilayer membrane which consisted of one layer of electrospun silk fibroin/PCL-PEG-PCL incorporating nanocalcium phosphate (SPCA)1 and one layer of PCL membrane was developed for GBR. To improve the osteoconductivity of membranes, nanosized calcium phosphate particles synthesized by Flame Spray Pyrolysis method were incorporated into membranes at 10% (wt) (SPCA10) and 20% (wt) (SPCA20) of the polymer content...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28864220/evaluation-of-pulp-response-to-novel-bioactive-glass-pulp-capping-materials
#19
Yunzi Long, Siyi Liu, Lin Zhu, Qiming Liang, Xiaofeng Chen, Yanmei Dong
INTRODUCTION: This study aimed to investigate dental pulp responses to novel bioactive glass (BG) pulp capping materials after direct pulp capping in vivo. METHODS: Novel BG pulp capping materials are composed of powder and fluid. The powder is BG (82.36% SiO2, 15.36% CaO, and 2.28% P2O5), and the fluid is provided in 2 kinds: (1) phosphate buffer solution (BG-PB) and (2) phosphate buffer solution with the addition of 1 wt% sodium alginate (BG-PB-SA). After mixing the powder and fluid, BG-PB and BG-PB-SA were prepared...
October 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28855762/a-comparison-of-human-dental-pulp-response-to-calcium-hydroxide-and-biodentine-as-direct-pulp-capping-agents
#20
Anushka Lalit Jalan, Manjusha M Warhadpande, Darshan M Dakshindas
CONTEXT: Direct pulp capping involves the placement of a biocompatible agent on pulp tissue that has been inadvertently exposed from traumatic injury or by iatrogenic means. AIM: To compare the human pulp response to calcium hydroxide and Biodentine as direct pulp-capping agents. MATERIALS AND METHODS: Pulp exposures were performed on the pulpal floor of forty human permanent premolars. The exposure sites were dressed with either Dycal or Biodentine as pulp-capping materials...
March 2017: Journal of Conservative Dentistry: JCD
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