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Guided Tissue Regeneration

Ravinder Bamba, Thanapong Waitayawinyu, Ratnam Nookala, David Colton Riley, Richard B Boyer, Kevin W Sexton, Chinnakart Boonyasirikool, Sunyarn Niempoog, Nathaniel D Kelm, Mark D Does, Richard D Dortch, Robert Bruce Shack, Wesley P Thayer
BACKGROUND: Peripheral nerve injury can have a devastating impact on our military and veteran population. Current strategies for peripheral nerve repair include techniques such as nerve tubes, nerve grafts, tissue matrices, and nerve growth guides to enhance the number of regenerating axons. Even with such advanced techniques, it takes months to regain function. In animal models, polyethylene glycol (PEG) therapy has shown to improve both physiologic and behavioral outcomes after nerve transection by fusion of a portion of the proximal axons to the distal axon stumps...
November 2016: Journal of Trauma and Acute Care Surgery
Richard J McMurtrey
Biomaterials are becoming an essential tool in the study and application of stem cell research. Various types of biomaterials enable three-dimensional culture of stem cells, and, more recently, also enable high-resolution patterning and organization of multicellular architectures. Biomaterials also hold potential to provide many additional advantages over cell transplants alone in regenerative medicine. This article describes novel designs for functionalized biomaterial constructs that guide tissue development to targeted regional identities and structures...
January 2016: Journal of Tissue Engineering
Carlos Parra, Y Natalie Jeong, Charles E Hawley
Recession on the lingual aspect of mandibular incisors may occur in patients with history of tongue piercing and other factitious traumatic habits. Treatment of these areas is challenging due to the site-specific anatomical features of the region. This case report presents a novel approach for a specific type of mandibular lingual defect caused by tongue piercing. A nonresorbable titanium-reinforced barrier membrane combined with an allograft and enamel matrix derivatives was used to promote regeneration of periodontal attachment...
November 2016: International Journal of Periodontics & Restorative Dentistry
Wenlong Liu, Xiuli Dan, Ting Wang, William Weijia Lu, Haobo Pan
The development of an optimal animal model which could provide fast assessments of the interaction between bone and orthopaedic implants is essential for both pre-clinical and theoretical researches in the design of novel biomaterials. Compared with other animal models, mice have superiority in accessing the well-developed transgenic modification techniques (e.g. cell tracing, knock-off, knock-in ect.) which serve as powerful tools in studying molecular mechanisms. In this study, we introduced the establishment of a mouse model which is specifically tailored for the assessment of bone-implant interaction in a load-bearing bone marrow microenvironment and could potentially allow the molecular mechanism study of biomaterials by using transgenic technologies...
October 12, 2016: Tissue Engineering. Part C, Methods
Ralf Smeets, Christine Knabe, Andreas Kolk, Michael Rheinnecker, Alexander Gröbe, Max Heiland, Rolf Zehbe, Manuela Sachse, Christian Große-Siestrup, Michael Wöltje, Henning Hanken
This study assesses the biocompatibility of novel silk protein membranes with and without modification, and evaluates their effect on facilitating bone formation and defect repair in guided bone regeneration. Two calvarian bone defects 12 mm in diameter were created in each of a total of 38 rabbits. Four different types of membranes, (silk-, hydroxyapatite-modified silk-, β-TCP-modified silk- and commonly clinically used collagen-membranes) were implanted to cover one of the two defects in each animal. Histologic analysis did not show any adverse tissue reactions in any of the defect sites indicating good biocompatibility of all silk protein membranes...
October 12, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Jairo A Diaz, Mauricio F Murillo, Jhonan A Mendoza, Ana M Barreto, Lina S Poveda, Lina K Sanchez, Laura C Poveda, Katherine T Mora
Emergent biological responses develop via unknown processes dependent on physical collision. In hypoxia, when the tissue architecture collapses but the geometric core is stable, actin cytoskeleton filament components emerge, revealing a hidden internal order that identifies how each molecule is reassembled into the original mold, using one common connection, i.e., a fractal self-similarity that guides the system from the beginning in reverse metamorphosis, with spontaneous self-assembly of past forms that mimics an embryoid phenotype...
2016: American Journal of Stem Cells
Shan Yu, Ying Gao, Xu Mei, Tanchen Ren, Su Liang, Zhengwei Mao, Changyou Gao
The selective adhesion and migration of endothelial cells (ECs) over smooth muscle cells (SMCs) is very important in the rapid endothelialization of blood-contacting implants, to prevent vascular restenosis. In this study, a uniform cell-resistant layer of methacrylate-functionalized hyaluronic acid (HA) was first immobilized on a poly(ε-caprolactone) (PCL) film via polydopamine coupling. Then, a density gradient of thiol-functionalized Arg-Glu-Asp-Val (REDV) peptide was prepared on the HA layer via thiol-ene click chemistry and the continuous injection method...
October 10, 2016: ACS Applied Materials & Interfaces
Daniel S Thoma, Nadja Naenni, Goran I Benic, Christoph H F Hämmerle, Ronald E Jung
AIM: To test whether or not soft tissue augmentation with a collagen matrix (VCMX) leads to a similar increase in ridge width around dental implants compared to the use of an autogenous subepithelial connective tissue graft (SCTG). MATERIALS AND METHODS: In 12 dogs, immediate dental implants were placed with simultaneous guided bone regeneration. Three months later, soft tissue volume augmentation was performed by randomly allocating three treatment modalities to these sites (VCMX, SCTG, sham-operated group (control))...
October 7, 2016: Journal of Clinical Periodontology
Henrique Almeida, Binulal Nelson Sathy, Ivan Dudurych, Conor Timothy Buckley, Fergal J O'Brien, Daniel John Kelly
Regenerating articular cartilage and fibrocartilaginous tissue such as the meniscus is still a challenge in orthopedic medicine. While a range of different scaffolds have been developed for joint repair, none have facilitated the development of a tissue that mimics the complexity of soft tissues such as articular cartilage. Furthermore, many of these scaffolds are not designed to function in mechanically challenging joint environments. The overall goal of this study was to develop a porous, biomimetic, shape-memory alginate scaffold for directing cartilage regeneration...
October 6, 2016: Tissue Engineering. Part A
Nanbo Liu, Sha Huang, Bin Yao, Jiangfan Xie, Xu Wu, Xiaobing Fu
3D bioprinting matrices are novel platforms for tissue regeneration. Tissue self-organization is a critical process during regeneration that implies the features of organogenesis. However, it is not clear from the current evidences whether 3D printed construct plays a role in guiding tissue self-organization in vitro. Based on our previous study, we bioprinted a 3D matrix as the restrictive niche for direct sweat gland differentiation of epidermal progenitors by different pore structure (300-μm or 400-μm nozzle diameters printed) and reported a long-term gradual transition of differentiated cells into glandular morphogenesis occurs within the 3D construct in vitro...
October 3, 2016: Scientific Reports
Anwarul Hasan, Ahmad Khattab, Mohammad Ariful Islam, Khaled Abou Hweij, Joya Zeitouny, Renae Waters, Malek Sayegh, Md Monowar Hossain, Arghya Paul
Cardiac tissue damage due to myocardial infarction (MI) is one of the leading causes of mortality worldwide. The available treatments of MI include pharmaceutical therapy, medical device implants, and organ transplants, all of which have severe limitations including high invasiveness, scarcity of donor organs, thrombosis or stenosis of devices, immune rejection, and prolonged hospitalization time. Injectable hydrogels have emerged as a promising solution for in situ cardiac tissue repair in infarcted hearts after MI...
November 2015: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Laura M Fluke, Ryan D Restrepo, Shyam Patel, Benjamin D Hoagland, Lori M Krevetski, Jacob T Stephenson
BACKGROUND: Full-thickness soft tissue defects from congenital absence or traumatic loss are difficult to surgically manage. Healing requires cell migration, organization of an extracellular matrix, inflammation, and wound coverage. PLCL (70:30 lactide:caprolactone, Purac), poly(propylene glycol) nanofibrous scaffolds enhance cell infiltration in vitro. This study compares strength and tissue ingrowth of aligned and unaligned nanofibrous scaffolds to absorbable and permanent meshes. We hypothesize that PLCL nanofibrous grafts will provide strength necessary for physiological function while serving as a scaffold to guide native tissue regeneration in vivo...
October 2016: Journal of Surgical Research
Elena Provaggi, Julian J H Leong, Deepak M Kalaskar
The latest and fastest-growing innovation in the medical field has been the advent of three-dimensional printing technologies, which have recently seen applications in the production of low-cost, patient-specific medical implants. While a wide range of three-dimensional printing systems has been explored in manufacturing anatomical models and devices for the medical setting, their applications are cutting-edge in the field of spinal surgery. This review aims to provide a comprehensive overview and classification of the current applications of three-dimensional printing technologies in spine care...
September 22, 2016: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
Joseph E Marturano, Nathan R Schiele, Zachary A Schiller, Thomas V Galassi, Matteo Stoppato, Catherine K Kuo
Tendon injuries heal as scar tissue with significant dysfunction and propensity to re-injure, motivating efforts to develop stem cell-based therapies for tendon regeneration. For these therapies to succeed, effective cues to guide tenogenesis are needed. Our aim is to identify these cues within the embryonic tendon microenvironment. We recently demonstrated embryonic tendon elastic modulus increases during development and is substantially lower than in adult. Here, we examined how these embryonic mechanical properties influence tenogenically differentiating cells, by culturing embryonic tendon progenitor cells (TPCs) within alginate gel scaffolds fabricated with embryonic tendon mechanical properties...
October 3, 2016: Journal of Biomechanics
Flora Gilde, Laure Fourel, Raphael Guillot, Isabelle Pignot-Paintrand, Takaharu Okada, Vincent Fitzpatrick, Thomas Boudou, Corinne Albiges-Rizo, Catherine Picart
: Surface coatings delivering BMP are a promising approach to render biomaterials osteoinductive. In contrast to soluble BMPs which can interact with their receptors at the dorsal side of the cell, BMPs presented as an insoluble cue physically bound to a biomimetic matrix, called here matrix-bound (bBMP-2), are presented to cells by their ventral side. To date, BMP-2 internalization and signaling studies in cell biology have always been performed by adding soluble (sBMP-2) to cells adhered on cell culture plates or glass slides, which will be considered here as a "reference" condition...
September 12, 2016: Acta Biomaterialia
Salazar Jones, Howard M Eisenberg, Xiaofeng Jia
Peripheral nerve injuries remain a significant source of long lasting morbidity, disability, and economic costs. Much research continues to be performed in areas related to improving the surgical outcomes of peripheral nerve repair. In this review, the physiology of peripheral nerve regeneration and the multitude of efforts to improve surgical outcomes are discussed. Improvements in tissue engineering that have allowed for the use of synthetic conduits seeded with neurotrophic factors are highlighted. Selected pre-clinical and available clinical data using cell based methods such as Schwann cell, undifferentiated, and differentiated stem cell transplantation to guide and enhance peripheral nerve regeneration are presented...
2016: International Journal of Molecular Sciences
Zeeshan Sheikh, Javairia Qureshi, Abdullah M Alshahrani, Heba Nassar, Yuichi Ikeda, Michael Glogauer, Bernhard Ganss
Certain cell populations within periodontal tissues possess the ability to induce regeneration, provided they have the opportunity to populate the wound or defect. Guided regeneration techniques have been investigated for regenerating periodontal tissues and such therapies usually utilize barrier membranes. Various natural and synthetic barrier membranes have been fabricated and tested to prevent epithelial and connective tissue cells from invading while allowing periodontal cells to selectively migrate into the defect...
September 9, 2016: Odontology
Alieh Aminian, Bahareh Shirzadi, Zahra Azizi, Kathrin Maedler, Eike Volkmann, Nils Hildebrand, Michael Maas, Laura Treccani, Kurosch Rezwan
Functional bone and dental implant materials are required to guide cell response, offering cues that provide specific instructions to cells at the implant/tissue interface while maintaining full biocompatibility as well as the desired structural requirements and functions. In this work we investigate the influence of covalently immobilized alkaline phosphatase (ALP), an enzyme involved in bone mineralization, on the first contact and initial cell adhesion. To this end, ALP is covalently immobilized by carbodiimide-mediated chemoligation on two highly bioinert ceramics, alpha-alumina (Al2O3) and yttria-stabilized zirconia (Y-TZP) that are well-established for load-bearing applications...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Suthira Owlarn, Kerstin Bartscherer
The unique ability of some planarian species to regenerate a head de novo, including a functional brain, provides an experimentally accessible system in which to study the mechanisms underlying regeneration. Here, we summarize the current knowledge on the key steps of planarian head regeneration (head-versus-tail decision, anterior pole formation and head patterning) and their molecular and cellular basis. Moreover, instructive properties of the anterior pole as a putative organizer and in coordinating anterior midline formation are discussed...
June 2016: Regeneration
Farrokh Sharifi, Bhavika B Patel, Adam K Dzuilko, Reza Montazami, Donald S Sakaguchi, Nastaran Hashemi
Fibrous scaffolds have shown promise in tissue engineering due to their ability to improve cell alignment and migration. In this paper, poly(ε-caprolactone) (PCL) fibers are fabricated in different sizes using a microfluidic platform. By using this approach, we demonstrated considerable flexibility in ability to control the size of the fibers. It was shown that the average diameter of the fibers was obtained in the range of 2.6-36.5 μm by selecting the PCL solution flow rate from 1 to 5 μL min(-1) and the sheath flow rate from 20 to 400 μL min(-1) in the microfluidic channel...
October 10, 2016: Biomacromolecules
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