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https://www.readbyqxmd.com/read/28654046/hypoxic-preconditioning-of-marrow-derived-progenitor-cells-as-a-source-for-the-generation-of-mature-schwann-cells
#1
Yat-Ping Tsui, Alan Kwan-Long Mung, Ying-Shing Chan, Daisy Kwok-Yan Shum, Graham Ka-Hon Shea
This manuscript describes a means to enrich for neural progenitors from the marrow stromal cell (MSC) population and thereafter to direct them to the mature Schwann cell fate. We subjected rat and human MSCs to transient hypoxic conditions (1% oxygen for 16 h) followed by expansion as neurospheres upon low-attachment substratum with epidermal growth factor (EGF)/basic fibroblast growth factor (bFGF) supplementation. Neurospheres were seeded onto poly-D-lysine/laminin-coated tissue culture plastic and cultured in a gliogenic cocktail containing β-Heregulin, bFGF, and platelet-derived growth factor (PDGF) to generate Schwann cell-like cells (SCLCs)...
June 14, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28648877/a-rare-presentation-a-case-report-of-osseous-metaplasia-and-mature-bone-formation-in-a-follicular-adenoma-of-the-thyroid
#2
Nadia Aurora, Insia Hashmi, Subhasis Misra, Nail Aydin
INTRODUCTION: Cases of multinodular goiter, thyroid hyperplasia, follicular adenoma, papillary thyroid carcinoma, and anaplastic thyroid carcinoma have been reported with histopathologic findings of osseous metaplasia (OM), bone marrow metaplasia (BMM), ectopic bone formation (EBF), ossification, and extramedullary hematopoiesis (EMH). To date no report of a follicular adenoma with OM and mature EBF in the absence of EMH has been reported in the English language. PRESENTATION OF CASE: 63-year-old woman with an incidental finding of thyroid nodule unable to be biopsied...
June 17, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28647850/wound-healing-protein-profiles-in-the-postoperative-exudate-of-bisphosphonate-related-osteonecrosis-of-mandible
#3
Soung Min Kim, Mi Young Eo, Yun Ju Cho, Yeon Sook Kim, Suk Keun Lee
Recurrent bacterial infections in cases of bisphosphonate-related osteonecrosis of jaw (BRONJ) frequently occur. Therefore, BRONJ are usually treated by radical saucerization followed by intensive antibiotic medications without bisphosphonate therapy. The postoperative exudate (POE) from BRONJ lesions may directly indicate the inflammatory status of osteomyelitis in patients, but so far, the POE has rarely been examined for its expression of various cytokines and wound healing proteins. A total of 27 cases of BRONJ, which involved the mandible, were selected and their individual POE collected 6 h, 1 day, and 2 days after surgical intervention was analyzed by immunoprecipitation high performance liquid chromatography (IP-HPLC)...
June 24, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28638147/sensitivity-of-human-pluripotent-stem-cells-to-insulin-precipitation-induced-by-peristaltic-pump-based-medium-circulation-considerations-on-process-development
#4
Diana Massai, Emiliano Bolesani, Diana Robles Diaz, Christina Kropp, Henning Kempf, Caroline Halloin, Ulrich Martin, Tudor Braniste, Giuseppe Isu, Vanessa Harms, Umberto Morbiducci, Gerald Dräger, Robert Zweigerdt
Controlled large-scale production of human pluripotent stem cells (hPSCs) is indispensable for their envisioned clinical translation. Aiming at advanced process development in suspension culture, the sensitivity of hPSC media to continuous peristaltic pump-based circulation, a well-established technology extensively used in hydraulically-driven bioreactors, was investigated. Unexpectedly, conditioning of low protein media (i.e. E8 and TeSR-E8) in a peristaltic pump circuit induced severe viability loss of hPSCs cultured as aggregates in suspension...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28629534/heparin-derived-oligosaccharide-inhibits-vascular-intimal-hyperplasia-in-balloon-injured-carotid-artery
#5
Jie-Ru Liu, Jie Wu, Xin-Chao Yu, Xuan Qian, Rui Xiong, Hui-Fang Wang, Dan-Feng Yu, Fei-Fei Liu, Shu-Ying He
The aims of the present study were to determine the effects of heparin-derived oligosaccharides (HDOs) on vascular intimal hyperplasia (IH) in balloon-injured carotid artery and to elucidate the underlying mechanisms of action. An animal model was established by rubbing the endothelia within the common carotid artery (CCA) in male rabbits. The rabbits were fed a high-cholesterol diet. Arterial IH was determined by histopathological changes to the CCA. Serum lipids were detected using an automated biochemical analysis...
June 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28624802/heparin-based-coacervate-of-bfgf-facilitates-peripheral-nerve-regeneration-by-inhibiting-endoplasmic-reticulum-stress-following-sciatic-nerve-injury
#6
Rui Li, Shuang Zou, Yanqing Wu, Yiyang Li, Sinan Khor, Yuqin Mao, Huacheng He, Ke Xu, Hongyu Zhang, Xiaokun Li, Jian Wang, Huai Jiang, Qike Jin, Qingsong Ye, Zhouguang Wang, Jian Xiao
Creating a microenvironment at the injury site that favors axonal regrowth and remyelinationis pivotal to the success of therapeutic reinnervation. The mature myelin sheath of the peripheral nervous system depends on active participation of Schwann cells to form new cytoskeletal components and tremendous amounts of relevant neurotrophic factors. In this study, we utilized a new biomaterial for growth factor delivery consisting of a biocompatible polycation, poly(ethylene argininylaspartatediglyceride) and heparin...
May 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28624705/controlled-release-of-basic-fibroblast-growth-factor-for-angiogenesis-using-acoustically-responsive-scaffolds
#7
Alexander Moncion, Melissa Lin, Eric G O'Neill, Renny T Franceschi, Oliver D Kripfgans, Andrew J Putnam, Mario L Fabiilli
The clinical translation of pro-angiogenic growth factors for treatment of vascular disease has remained a challenge due to safety and efficacy concerns. Various approaches have been used to design spatiotemporally-controlled delivery systems for growth factors in order to recapitulate aspects of endogenous signaling and thus assist in translation. We have developed acoustically-responsive scaffolds (ARSs), which are fibrin scaffolds doped with a payload-containing, sonosensitive emulsion. Payload release can be controlled non-invasively and in an on-demand manner using focused, megahertz-range ultrasound (US)...
June 9, 2017: Biomaterials
https://www.readbyqxmd.com/read/28619014/prognostic-impact-of-blood-and-urinary-angiogenic-factor-levels-at-diagnosis-and-during-treatment-in-patients-with-osteosarcoma-a-prospective-study
#8
Marie-Dominique Tabone, Laurence Brugières, Sophie Piperno-Neumann, Marie-Ange Selva, Perrine Marec-Bérard, Hélène Pacquement, Cyril Lervat, Nadège Corradini, Jean-Claude Gentet, Rémy Couderc, Aurélie Chevance, Céline Mahier-Ait Oukhatar, Natacha Entz-Werle, Jean-Yves Blay, Marie-Cecile Le Deley
BACKGROUND: Angiogenesis is essential for the progression and metastatic spread of solid tumours. Expression of vascular endothelial growth factor (VEGF) has been linked to poor survival among osteosarcoma patients but the clinical relevance of monitoring blood and urine angiogenic factors is uncertain. The aim of this study was to determine the prognostic significance of blood VEGF and blood and urinary basic fibroblast growth factor (bFGF) levels in osteosarcoma patients, both at diagnosis and during treatment...
June 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28617813/identification-of-padi2-as-a-novel-angiogenesis-regulating-gene-by-genome-association-studies-in-mice
#9
Mehrdad Khajavi, Yi Zhou, Amy E Birsner, Lauren Bazinet, Amanda Rosa Di Sant, Alex J Schiffer, Michael S Rogers, Subrahmanian Tarakkad Krishnaji, Bella Hu, Vy Nguyen, Leonard Zon, Robert J D'Amato
Recent findings indicate that growth factor-driven angiogenesis is markedly influenced by genetic variation. This variation in angiogenic responsiveness may alter the susceptibility to a number of angiogenesis-dependent diseases. Here, we utilized the genetic diversity available in common inbred mouse strains to identify the loci and candidate genes responsible for differences in angiogenic response. The corneal micropocket neovascularization assay was performed on 42 different inbred mouse strains using basic fibroblast growth factor (bFGF) pellets...
June 15, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28615811/adipokine-concentrations-in-lipoaspirates-may-have-a-role-in-wound-healing
#10
Christian Herold, Stefan Engeli, Bibiana Beckmann, Peter M Vogt, Hans-Oliver Rennekampff
OBJECTIVES: In addition to its use as a volume filler, fat grafting may have a potential role in wound healing based on the concentration of growth factors in the lipoaspirate. In this study, we compare the quantitative and qualitative concentration of the various growth factors and adipokines using the Shippert or the Coleman techniques to prepare the lipoaspirate. METHODS: We measured leptin, adiponectin and the growth factors, i.e., acidic fibroblast growth factor (aFGF), basic FGF (bFGF), keratinocyte growth factor (KGF), bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) by ELISA in solid and liquid fractions obtained with both techniques in human fat obtained with Coleman technique and Shippert technique...
January 2017: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/28614916/gestational-diabetes-affects-the-growth-and-functions-of-perivascular-stem-cells
#11
Borim An, Eunbi Kim, Haengseok Song, Kwon-Soo Ha, Eun-Taek Han, Won Sun Park, Tae Gyu Ahn, Se-Ran Yang, Sunghun Na, Seok-Ho Hong
Gestational diabetes mellitus (GDM), one of the common metabolic disorders of pregnancy, leads to functional alterations in various cells including stem cells as well as some abnormalities in fetal development. Perivascular stem cells (PVCs) have gained more attention in recent years, for the treatment of various diseases. However, the effect of GDM on PVC function has not been investigated. In our study, we isolated PVCs from umbilical cord of normal pregnant women and GDM patients and compared their phenotypes and function...
June 13, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28606816/altered-distribution-of-peripheral-blood-dendritic-cell-subsets-in-patients-with-pulmonary-paracoccidioidomycosis
#12
James Venturini, Ricardo Souza Cavalcante, Daniela Vanessa Moris, Márjorie de Assis Golim, Adriele Dandara Levorato, Karoline Hagatha Dos Reis, Maria Sueli Parreira de Arruda, Rinaldo Poncio Mendes
Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi from the genus Paracoccidioides in Latin America. PCM-patients (PCM-p) are classified as having acute/subacute or chronic (CF) clinical forms. CF is responsible for 75% - 90% of all cases, affects mainly adults over 30 years old and the clinical manifestation are associated mainly with lungs and mucosa of upper airdigestive tract. In addition, the CF patients exhibit fibrosis of the lungs, oral mucous membranes and adrenals, and pulmonary emphysema...
June 9, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28602978/tanshinone-iia-inhibits-%C3%AE-catenin-vegf-mediated-angiogenesis-by-targeting-tgf-%C3%AE-1-in-normoxic-and-hif-1%C3%AE-%C3%A2-in-hypoxic-microenvironments-in-human-colorectal-cancer
#13
Hua Sui, Jihui Zhao, Lihong Zhou, Haotian Wen, Wanli Deng, Chunpu Li, Qing Ji, Xuan Liu, Yuanyuan Feng, Ni Chai, Qibo Zhang, Jianfeng Cai, Qi Li
In a previous study, we demonstrated that Tanshinone IIA effectively inhibits CRC angiogenesis in vivo, but the underlying mechanisms were not elucidated. In this report, we describe experiments in which HIF-1α levels were manipulated to probe the effect of hypoxia on CRC cell angiogenesis. We studied the effects of Tan IIA on CRC pro-angiogenic factor and on human umbilical vein endothelial cell angiogenesis in normoxia and hypoxia. Our results show that Tan IIA not only lowers HIF-1α levels and inhibits secretion of VEGF and bFGF, but also efficiently suppresses the proliferation, tube formation and metastasis of HUVECs...
June 7, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28601638/the-anti-growth-and-anti-metastasis-effects-of-schisandrin-b-on-hepatocarcinoma-cells-in-vitro-and-in-vivo
#14
Ruijie Sun, Ruiren Zhai, Changlin Ma, Miao Wei
Hepatocarcinoma (HCC) is the most common liver cancer with high metastasis and recurrence rate which represents extremely poor prognosis. In this study, we aimed to investigate the effects of Schisandrin B (Sch B) on HCC cells both in vitro and in vivo and to explore its underlying mechanism. We found that Sch B inhibited the proliferation of HCC cells in a dose-dependent manner as assessed by CCK-8 assay. The flow cytometric assay showed that Sch B significantly induced HCC cell apoptosis. Besides, Sch B suppressed HCC cell metastasis as detected by both wound-healing assay and transwell migration assay...
June 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28595579/omental-adipose-tissue-is-a-more-suitable-source-of-canine-mesenchymal-stem-cells
#15
Francisca Bahamondes, Estefania Flores, Gino Cattaneo, Flavia Bruna, Paulette Conget
BACKGROUND: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue...
June 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28595487/circulating-levels-of-vascular-endothelial-growth-factor-and-basic-fibroblastic-growth-factor-in-infantile-hemangioma-versus-vascular-malformations
#16
Nehal M El-Raggal, Rania A El-Farrash, Abeer A Saad, Enas A S Attia, Hatem A Saafan, Ibrahim S Shaaban
Few preliminary reports studied the utility of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) for differentiation between infantile hemangiomas (IHs) and vascular malformations. The aim of this study was to investigate the role of serum VEGF and bFGF levels in differentiating IHs from vascular malformations and identifying the stage and clinical course of IHs. Serum levels of VEGF and bFGF were assessed in 60 infants and children with various cutaneous vascular anomalies defined in 3 groups: proliferating IHs (n = 25), involuting IHs (n = 23), and vascular malformations (n = 12), in comparison with their levels in 40 healthy matched control...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/28593712/human-adipose-tissue-derived-tenomodulin-positive-subpopulation-of-stem-cells-a-promising-source-of-tendon-progenitor-cells
#17
A I Gonçalves, P M Gershovich, M T Rodrigues, R L Reis, M E Gomes
Cell-based therapies are of particular interest for tendon and ligament regeneration given the low regenerative potential of these tissues. Adipose tissue is an abundant source of stem cells, which may be employed for the healing of tendon lesions. However, human adult multipotent adipose-derived stem cells (hASCs) isolated from the stromal vascular fraction (SVF) of adipose tissue originate highly heterogeneous cell populations that hinder their use in specific tissue-oriented applications. In this study, distinct subpopulations of hASCs were immunomagnetic separated and their tenogenic differentiation capacity evaluated in the presence of several growth factors (GFs), namely EGF, bFGF, TGF-β1 and PDGF-BB, that are well-known regulators of tendon development, growth and healing...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28592031/-the-stimulation-of-human-pulmonary-artery-endothelial-cells-by-cigarette-smoke-extract-contributed-to-cell-senescence-and-induced-human-pulmonary-artery-smooth-cell-migration
#18
L Cai, P C Zhu, Y E Wang, Y T Gao, Q L Ao
Objective: To observe the senescent effect of human pulmonary arterial endothelial cells (HPAEC) stimulated by cigarette smoke extract (CSE) and the effect of secretion of senescent cells on human pulmonary arterial smooth muscles cell (HPASMC) proliferation and migration. Methods: HPAEC was treated with different concentrations of CSE in vitro and cell proliferation was determined by CCK8, senescence cells analyzed by detecting the β-gal activity, and the senescent proteins of cells measured by Western blot...
June 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28587326/degradation-of-mg-6zn-alloy-stents-does-not-influence-the-healing-of-the-common-bile-duct-in-vivo
#19
Yigang Chen, Nianfeng Sun, Jie Zhang, Shaoxiang Zhang, Changli Zhao, Jiazeng Xia
To investigate the effects of Mg-6Zn alloy on the healing of the common bile duct (CBD), Mg-6Zn alloy stents were implanted into the CBDs of rabbits. Stainless steel stents were transplanted into a second group of rabbits to serve as a control. Computed tomography (CT) scanning was performed and weight loss was recorded to evaluate the in vivo degradation process. Hematoxylin and eosin staining and immunohistochemical analyses were performed to determine the expressions of transforming growth factor (TGF)-β, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) and evaluate CBD healing...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28581882/nutriproteomic-analysis-of-hwangmaemok-induced-antiangiogenic-effect-using-antibody-arrayed-protein-chip-assay
#20
Yu Mi Park, Min-A Kim, Hee Tae Jung, Hwa Jeong Kang, Hwa-Seung Yoo, In-Cheol Kang
We investigated the antiangiogenic effects of Lindera obtusiloba Blume (Hwangmaemok, HMM), which is a plant in the Lauraceae family that is commonly used to treat colds and gastritis. Moreover, given that a recent study reported the inhibitory effects of HMM extract on cancer metastasis, we hypothesized that HMM extract might possess and antiangiogenic function. Thus, this study was conducted to investigate the effects of HMM extract on endothelial cell proliferation, migration, and neovascularization in chick chorioallantoic membrane (CAM), and investigated the molecular mechanism of antiangiogenesis using a ProteoChip-based proteomics technology...
June 2017: Journal of Medicinal Food
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