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M Cimino, R M Gonçalves, E Bauman, M Barroso-Vilares, E Logarinho, C C Barrias, M C L Martins
Human bone marrow (BM)-derived mesenchymal stem/stromal cells (hMSCs) are considered promising therapeutic agents in the field of cell therapy and regenerative medicine, mainly due to their relative facility to be isolated, multi-differentiation potential and immunomodulatory role. However, their application in clinics requires a crucial step of in vitro expansion. Most of the protocols for hMSCs in vitro culture use foetal bovine serum (FBS) as medium supplement that, being from animal origin, presents several safety concerns and may initiate xenogeneic immune responses after cells transplantation...
October 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Satomi Suzuki Uematsu, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Yasuhiro Igarashi, Ibrahim Fathi, Shigehito Miyagi, Noriaki Ohuchi, Susumu Satomi, Masafumi Goto
BACKGROUND: Subcutaneous islet transplantation (SC Tx) is associated with minimal invasiveness, but poor vascularization. Thus, the optimization of the prevascularization procedures is crucial for improving the outcomes. Although the effectiveness of basic fibroblast growth factor (bFGF) was reported, the optimal procedures remain unclear. We sought to optimize the prevascularization procedures including the use of a novel scaffold, recombinant peptide (RCP). METHODS: Devices containing various amount of bFGF with/without heparin, or RCP were implanted into the subcutaneous space of diabetic C57BL/6 mice...
October 10, 2017: Transplantation
Hui-Cong Du, Lin Jiang, Wen-Xin Geng, Jing Li, Rui Zhang, Jin-Ge Dang, Mao-Guo Shu, Li-Wen Li
MSC treatment can promote cutaneous wound repair through multiple mechanisms, and paracrine mediators secreted by MSC are responsible for most of its therapeutic benefits. Recently, MSC sheet composed of live MSCs and their secreted ECMs was reported to promote wound healing; however, whether its ECM alone could accelerate wound closure remained unknown. In this study, Nc-ECM and Cc-ECM were prepared from nonconditioned and CoCl2-conditioned MSC sheets, respectively, and their wound healing properties were evaluated in a mouse model of full-thickness skin defect...
2017: BioMed Research International
Jiayang Wan, Haofang Wan, Rongbin Yang, Haitong Wan, Jiehong Yang, Yu He, Huifen Zhou
ETHNOPHARMACOLOGICAL RELEVANCE: Danhong Injection (DHI) and Naoxintong Capsule (NXT) are renowned traditional Chinese medicine in China. The drug combination of DHI and NXT is frequently applied for the treatment of cardiovascular and cerebrovascular diseases in clinic. However, there had been no pharmacological experiment studies of interaction between DHI and NXT. Due to the drug interactions, exploring their interaction profile is of great importance. MATERIAL AND METHODS: In this study, focal cerebral I/R injury in adult male Sprague-Dawley rats were induced by transient middle cerebral artery occlusion (tMCAO) for 1h followed by reperfusion...
October 3, 2017: Journal of Ethnopharmacology
Z-J Tian, B-Y Liu, Y-T Zhang, X-Z Chen, G-Y Qiao, S Wang, Z-L Ma
OBJECTIVE: To investigate the role of miR-145 silencing in the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), B-cell lymphoma-2 (Bcl-2), BCL2-Associated X(bax) and caspase-3 in avascular necrosis of femoral head (ANFH). MATERIALS AND METHODS: A total of 12 healthy wild-types (the control group) and 12 miR-145 knock-out (miR-145-/-) (the experimental group) adult New Zealand white rabbits were selected to construct ANFH model with a steroid...
October 2017: European Review for Medical and Pharmacological Sciences
Yusuke Shiode, Yuki Morizane, Ryo Matoba, Masayuki Hirano, Shinichiro Doi, Shinji Toshima, Kosuke Takahashi, Ryoichi Araki, Yuki Kanzaki, Mika Hosogi, Tomoko Yonezawa, Atsushi Yoshida, Fumio Shiraga
Purpose: To investigate the mechanism of macular hole (MH) closure following the inverted internal limiting membrane (ILM) technique. Methods: We performed the inverted ILM flap surgical technique as an experimental MH model in monkeys, and investigated the process of MH closure immunohistochemically. We then investigated the effects of type IV collagen, fibronectin, and laminin, which are constituent proteins of the ILM, on the proliferation and migration of cultivated Müller cells (MIO-M1)...
September 1, 2017: Investigative Ophthalmology & Visual Science
Hitoshi Nishiwaki, Mitsugu Fujita, Makoto Yamauchi, Noritaka Isogai, Yasuhiko Tabata, Hirohisa Kusuhara
Cartilage tissue is characterized by its poor regenerative properties, and the clinical performance of cartilage grafts to replace cartilage defects has been unsatisfactory. Recently, cartilage regeneration with mature chondrocytes and stem cells has been developed and applied in clinical settings. However, there are challenges with the use of mature chondrocytes and stem cells for tissue regeneration, including the high costs associated with the standard stem cell isolation methods and the decreased cell viability due to cell manipulation...
October 4, 2017: Cells, Tissues, Organs
Jacob New, Levi Arnold, Megha Ananth, Sameer Alvi, Mackenzie Thornton, Lauryn R Werner, Ossama Tawfik, Hongying Dai, Yelizaveta Shnayder, Kiran Kakarala, Terance Ted Tsue, Douglas A Girod, Wen-Xing Ding, Shrikant Anant, Sufi M Thomas
Despite therapeutic advancements, there has been little change in the survival of patients with head and neck squamous cell carcinoma (HNSCC). Recent results suggest that cancer-associated fibroblasts (CAF) drive progression of this disease. Here, we report that autophagy is upregulated in HNSCC-associated CAFs where it is responsible for key pathogenic contributions in this disease. Autophagy is fundamentally involved in cell degradation, but there is emerging evidence that suggests it is also important for cellular secretion...
September 28, 2017: Cancer Research
Shin-Jeong Lee, Young-Doug Sohn, Adinarayana Andukuri, Sangsung Kim, Jaemin Byun, Ji Woong Han, In-Hyun Park, Ho-Wook Jun, Young-Sup Yoon
Background -Human pluripotent stem cell (hPSC)-derived endothelial cells (ECs) have limited clinical utility due to undefined components in the differentiation system and poor cell survival in vivo Here, we aimed to develop a fully defined and clinically compatible system to differentiate hPSCs into ECs. Further, we aimed to enhance cell survival, vessel-formation, and therapeutic potential by encapsulating hPSC-ECs with a peptide amphiphile (PA) nanomatrix gel. Methods -We induced differentiation of hPSCs into the mesodermal lineage by culturing on collagen-coated plates with a GSK3β inhibitor...
September 29, 2017: Circulation
Yo-Chen Chang, Chia-Wei Lin, Ming-Chu Hsieh, Horng-Jiun Wu, Wen-Sheng Wu, Wen-Chuan Wu, Ying-Hsien Kao
Hypoxia-induced retinal neovascularization plays a central role in the pathogenesis of diabetic retinopathy. This study aimed to investigate whether hypoxia leads to the release of nuclear high mobility group box 1 (HMGB1) peptides from cultured retinal pigment epithelial ARPE-19 cells, to determine the effect of HMGB1 on angiogenic cytokine production and elucidate the involved signaling pathways. A chemical hypoxia mimetic agent, cobalt chloride, induced SIRT1 downregulation, HMGB1 nucleocytoplasmic relocation and extracellular release from ARPE-19 cells, implicating its autocrine function...
September 29, 2017: Cellular Signalling
Wooyoung Kim, Manse Kim, Giyoong Tae
Poly (γ-glutamic acid) (PGA) is a natural biomaterial with numerous good properties such as non-toxicity, non-immunogenicity, and water holding. So, various forms of PGA-based materials have been developed for bio-applications, including sheet, cross-linked hydrogel, and nanoparticle except injectable hydrogel type. Considering inherent advantages of injectable system, injectable hydrogel based on PGA can broaden the bio-application range of PGA. In this study, a PGA-based injectable hydrogel system was prepared by physically mixing it with a small amount of chitosan with two different water solubility and molecular weight...
September 29, 2017: International Journal of Biological Macromolecules
Jing Yang, Weidong Qiang, Suping Ren, Shanyong Yi, Jian Li, Lili Guan, Linna Du, Yongxin Guo, Huilong Hu, Haiyan Li, Xiaokun Li
Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factors family. It is a highly specific mitogenic factor for many cell types, as though it be involved in wound repair, angiogenesis, nerve nutrition and embryonic development etc. Oil bodies have been applied for medicine, foodstuff and industry field. The heterogonous proteins expressed in oil bodies have distinct advantages, such as less purification steps and low costs. In this study, bFGF was expressed in A. thaliana seeds using oleosin fusion technology...
September 29, 2017: Gene
Yuancheng Li, Qinbo Fan, Yong Jiang, Fuliang Gong, Honggang Xia
An integrated microfluidic device was utilized in the present study to investigate the morphology and proliferation of rabbit articular chondrocytes embedded in Matrigel in the presence of insulin-like growth factor 1 (IGF-1) and/or basic fibroblast growth factor (bFGF). The microfluidic device was composed of two parallel channels and a central perfusion-based three-dimensional cell culture module. The rabbit chondrocytes were cultured for 2 weeks at series of concentration gradients of IGF-1 and/or bFGF, which were generated through a diffusion process...
September 2017: Experimental and Therapeutic Medicine
Bibiane Steinecker-Frohnwieser, Heike Kaltenegger, Lukas Weigl, Anda Mann, Werner Kullich, Andreas Leithner, Birgit Lohberger
BACKGROUND: Osteoarthritis (OA) as the main chronic joint disease arises from a disturbed balance between anabolic and catabolic processes leading to destructions of articular cartilage of the joints. While mechanical stress can be disastrous for the metabolism of chondrocytes, mechanical stimulation at the physiological level is known to improve cell function. The disease modifying OA drug (DMOAD) diacerein functions as a slowly-acting drug in OA by exhibiting anti-inflammatory, anti-catabolic, and pro-anabolic properties on cartilage...
September 2017: Biochemistry and Biophysics Reports
Zhuoli Zhu, Xueqi Gan, Haiyang Yu
Potent paracrine properties, such as secretion of angiogenic cytokines and growth factors, have been considered essential for the function of mesenchymal stem cells (MSCs) in tissue regeneration and repair. The present study determined that bone marrow‑derived mesenchymal stem cells from mice fed a high fat diet (HFD) had reduced pro‑angiogenic capacity, as evident from the reduced expression of vascular endothelial growth factor A (VEGFA) and basic fibroblast growth factor (bFGF); therefore, a reduced number of branches was induced in the angiogenesis assay...
September 18, 2017: Molecular Medicine Reports
Amy Anne D Lassig, Joan E Bechtold, Bruce R Lindgren, Andrew Pisansky, Abayo Itabiyi, Bevan Yueh, Anne M Joseph
OBJECTIVE: Smoking impairs wound healing, yet the underlying pathophysiological mechanisms are unclear. We evaluated tobacco-altered healing in head and neck surgery by studying the association between biomarkers and tobacco exposure, as well as cutaneous perfusion by smoking status. STUDY DESIGN: Prospective cohort study, tertiary/academic care center, 2011 to present. METHODS: Patients who required head and neck surgery were enrolled prospectively...
September 20, 2017: Laryngoscope
Roghayeh Mokhtari Asl, Pegah Ghoraeian, Abbas Monzavi, Abbas Bahador
BACKGROUND: Periodontal diseases are pathological inflammatory conditions of multifactorial etiology that develops in response to periodontopathic bacteria. Recently, photodynamic therapy (PDT) has begun to be incorporated as an adjuvant treatment for periodontal diseases. However, the effect of PDT on tissue regeneration is not yet completely clear. This study aimed to analyze the gene expression of basic fibroblast growth factor (bFGF) following PDT in human gingival fibroblasts (HuGu) cells in vitro...
September 18, 2017: Photodiagnosis and Photodynamic Therapy
Mohd Fahami Nur Azlina, Hj Mohd Saad Qodriyah, Kien Hui Chua, Yusof Kamisah
AIM: To investigate and compare the effects of tocotrienol and omeprazole on gastric growth factors in rats exposed to water-immersion restraint stress (WIRS). METHODS: Twenty-eight male Wistar rats were randomly assigned to four groups of seven rats. The two control groups were administered vitamin-free palm oil (vehicle) and the two treatment groups were given omeprazole (20 mg/kg) or tocotrienol (60 mg/kg) by oral gavage. After 28 d of treatment, rats from one control group and both treated groups were subjected to WIRS one time for 3...
August 28, 2017: World Journal of Gastroenterology: WJG
Shan Wang, Miaohua Mo, Jinmei Wang, Sobia Sadia, Bihua Shi, Xiaobing Fu, Lin Yu, Edward E Tredget, Yaojiong Wu
Mesenchymal stem cells (MSCs) are heterogeneous likely consisting of subpopulations with various therapeutic potentials. Here we attempted to acquire a subset of MSCs with enhanced effect in wound healing. We found that human placental MSCs expressing platelet-derived growth factor (PDGF) receptor (PDGFR)-β exhibited greater proliferation rates and generated more colony-forming unit-fibroblast (CFU-F), compared to PDGFR-β(-) MSCs. Notably, PDGFR-β(+) MSCs expressed higher levels of pro-angiogenic factors such as Ang1, Ang2, VEGF, bFGF and PDGF...
September 19, 2017: Cellular and Molecular Life Sciences: CMLS
Lin Wang, Wei-Zhi Liu, Ling-Yan Pei, Yu-Shi Ke, Jian Cui, Shu-Chun Li
Garidisan, commonly used in Mongolia to treat ulcerative colitis (UC), contains wild poppy and Artemisia frigida Willd. Clinical evidence shows that Garidisan can effectively treat UC and that recurrence is low. Thus, we evaluated the effects of Garidisan on ulcer healing quality and the regulation of immune balance in rats with experimental UC. UC was induced by immunization with TNBS and Garidisan significantly reduced DAI, CMDI, and HS. H&E staining, SEM, and VG staining showed that Garidisan repaired damaged intestinal mucosa and significantly reduced expression of ICAM-1 and CD105 in regenerated tissues of UC rats...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
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