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https://www.readbyqxmd.com/read/28306194/electrospun-biodegradable-microfibers-induce-new-collagen-formation-in-a-rat-abdominal-wall-defect-model-a-possible-treatment-for-pelvic-floor-repair
#1
Cecilie Glindtvad, Menglin Chen, Jens Vinge Nygaard, Lise Wogensen, Axel Forman, Carl Christian Danielsen, Mehmet Berat Taskin, Karl-Erik Andersson, Susanne Maigaard Axelsen
Half of the female population over age 50 years will experience pelvic organ prolapse. We suggest a new approach based on tissue engineering principles to functionally reconstruct the anatomical structures of the pelvic floor. The aim of this study is to investigate the mechanical performance and effect on collagen and elastin production of a degradable mesh releasing basic fibroblast growth factor (bFGF). Implantation of biodegradable mesh with or without bFGF in their core has been conducted in 40 rats in an abdominal wall defect model...
March 17, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28300551/role-of-caveolin-1-vascular-endothelial-growth-factor-pathway-in-basic-fibroblast-growth-factor-induced-angiogenesis-and-neurogenesis-after-treadmill-training-following-focal-cerebral-ischemia-in-rats
#2
Qiongyi Pang, Huimei Zhang, Zhenzhen Chen, Yudan Wu, Min Yidian Bai Liu, Yun Zhao, Fengxia Tu, Chan Liu, Xiang Chen
Exercise is known to aid functional recovery following ischemia, though the mechanisms responsible for the beneficial effects of exercise on recovery from ischemic stroke are not fully understood. Basic fibroblast growth factor (bFGF) contributes to angiogenesis and promotes neurologic functional recovery after stroke. The present study aimed to investigate the possible mechanisms whereby treadmill exercise ameliorated impaired angiogenesis and neurogenesis following transient cerebral ischemia in middle cerebral artery occlusion (MCAO) rats...
March 11, 2017: Brain Research
https://www.readbyqxmd.com/read/28298893/bis-retinoid-a2e-induces-an-increase-of-basic-fibroblast-growth-factor-via-inhibition-of-extracellular-signal-regulated-kinases-1-2-pathway-in-retinal-pigment-epithelium-cells-and-facilitates-phagocytosis
#3
Delphine Balmer, Linda Bapst-Wicht, Aswin Pyakurel, Martine Emery, Natacha Nanchen, Christian G Bochet, Raphael Roduit
Age-related macular degeneration (ARMD) is the leading cause of vision loss in developed countries. Hallmarks of the disease are well known; indeed, this pathology is characterized by lipofuscin accumulation, is principally composed of lipid-containing residues of lysosomal digestion. The N-retinyl-N-retinylidene ethanolamine (A2E) retinoid which is thought to be a cytotoxic component for RPE is the best-characterized component of lipofuscin so far. Even if no direct correlation between A2E spatial distribution and lipofuscin fluorescence has been established in aged human RPE, modified forms or metabolites of A2E could be involved in ARMD pathology...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28293476/evaluating-the-levels-of-csf-and-serum-factors-in-als
#4
Jie Guo, Xuan Yang, Lina Gao, Dawei Zang
OBJECTIVES: The aim of this study was to identify CSF and serum factors as biomarkers that may aid in distinguishing ALS patients from control subjects and predicting ALS progression as well as prognosis. METHODS: Serum and CSF samples from 105 patients with ALS and 56 control subjects were analyzed for 13 factors using ELISA. The revised ALS functional rating scale (ALSFRS-r) was used to evaluate the overall functional status of ALS patients, and we also followed up with ALS patients either by phone or with clinic visits for five years after enrollment in this study...
March 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28291798/implantable-porous-gelatin-microspheres-sustained-release-of-bfgf-and-improved-its-neuroprotective-effect-on-rats-after-spinal-cord-injury
#5
Li Lan, Fu-Rong Tian, De-Li ZhuGe, Qi-Chuan ZhuGe, Bi-Xin Shen, Bing-Hui Jin, Jian-Ping Huang, Ming-Ze Wu, Lu-Xin Fan, Ying-Zheng Zhao, He-Lin Xu
In this study, porous gelatin microspheres (GMSs) were constructed to improve the neuroprotective effect of basic fibroblast growth factor (bFGF) on spinal cord injury. GMSs were prepared by a W/O emulsion template, followed by cross-linking, washing and drying. The particle sizes and surface porosity of the blank GMSs were carefully characterized by scan electronic microscopy. The blank GMSs have a mean particle size of 35μm and theirs surface was coarse and porous. bFGF was easily encapsulated inside the bulk GMSs through diffusion along the porous channel...
2017: PloS One
https://www.readbyqxmd.com/read/28290607/homemade-device-induced-negative-pressure-promotes-wound-healing-more-efficiently-than-vsd-induced-positive-pressure-by-regulating-inflammation-proliferation-and-remodeling
#6
Jinyan Liu, Feng Hu, Jintian Tang, Shijie Tang, Kun Xia, Song Wu, Chaoqi Yin, Shaohua Wang, Quanyong He, Huiqing Xie, Jianda Zhou
Vacuum sealing drainage (VSD) is an effective technique used to promote wound healing. However, recent studies have shown that it exerts positive pressure (PP) rather than negative pressure (NP) on skin. In this study, we created a homemade device that could maintain NP on the wound, and compared the therapeutic effects of VSD-induced PP to those of our homemade device which induced NP on wound healing. The NP induced by our device required less time for wound healing and decreased the wound area more efficiently than the PP induced by VSD...
March 13, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28289610/effect-of-basic-fibroblast-growth-factor-on-achilles-tendon-healing-in-rabbit
#7
Arash Najafbeygi, Mohammad Javad Fatemi, Amir Hussein Lebaschi, Seyed Jaber Mousavi, Seyed Abouzar Husseini, Mitra Niazi
BACKGROUND: Tendon injuries are common and it takes a long time for an injured tendon to heal. Adverse phenomena such as adhesion and rupture are associated with these injuries. Finding a method to reduce the time required for healing which improves the final outcome, will lead to decreased frequency and intensity of adverse consequences. This study was designed to investigate the effects of basic fibroblast growth factor on the healing of the Achilles tendon in rabbits. METHODS: In 10 New Zealand white rabbits, Achilles tendon was cut at the intersection of the distal and middle thirds on both hind legs...
January 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28287635/efficient-production-of-trophoblast-lineage-cells-from-human-induced-pluripotent-stem-cells
#8
Junya Kojima, Atsushi Fukuda, Hayato Taira, Tomoyuki Kawasaki, Hiroe Ito, Naoaki Kuji, Keiichi Isaka, Akihiro Umezawa, Hidenori Akutsu
Human induced pluripotent stem cells (hiPSCs) are potentially useful in both clinical applications and basic biological research. hiPSCs can differentiate into extra-embryonic cells in the presence of BMP4. However, the differentiation potential of hiPSCs can be affected by culture conditions or genetic variation. In this study, we investigated the effect of various BMP4 concentrations on the expression states of trophoblast markers and the optimal conditions for trophoblast induction. A high-fidelity gene expression assay using hiPSC lines showed that the expression levels of various trophoblast marker genes, such as KRT7, GCM1, CGB, and HLA-G, were upregulated by BMP4 in a dose-dependent manner in all types of hiPSCs used in this study...
March 13, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28277063/quantification-of-growth-factors-in-different-platelet-concentrates
#9
Jing Qiao, Na An, Xiangying Ouyang
Concentrated growth factor (CGF), a new generation of platelet concentrate product, appears to have more abundant growth factors because of its special centrifugation process. However, there are few studies supporting this. This study was designed to evaluate the contents of major growth factors in CGF and compare them with those found in PRP (platelet-rich plasma) and PRF (platelet-rich fibrin). PRP, PRF, and CGF were obtained from the same samples of peripheral blood. Concentrations of five representative growth factors in platelets were measured with enzyme-linked immunosorbent assay (ELISA): platelet-derived growth factor-BB (PDGF-BB), transforming growth factor β-1 (TGF-β1), insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF)...
February 21, 2017: Platelets
https://www.readbyqxmd.com/read/28275653/ph20-is-not-expressed-in-murine-cns-and-oligodendrocyte-precursor-cells
#10
Mathieu Marella, Joe Ouyang, Jonathan Zombeck, Chunmei Zhao, Lei Huang, Robert J Connor, Kim B Phan, Michael C Jorge, Marie A Printz, Rudolph D Paladini, Arnold B Gelb, Zhongdong Huang, Gregory I Frost, Barry J Sugarman, Lawrence Steinman, Ge Wei, H Michael Shepard, Daniel C Maneval, Paula J Lapinskas
OBJECTIVE: Expression of Spam1/PH20 and its modulation of high/low molecular weight hyaluronan substrate have been proposed to play an important role in murine oligodendrocyte precursor cell (OPC) maturation in vitro and in normal and demyelinated central nervous system (CNS). We reexamined this using highly purified PH20. METHODS: Steady-state expression of mRNA in OPCs was evaluated by quantitative polymerase chain reaction; the role of PH20 in bovine testicular hyaluronidase (BTH) inhibition of OPC differentiation was explored by comparing BTH to a purified recombinant human PH20 (rHuPH20)...
March 2017: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/28259958/comparison-of-the-proliferation-migration-and-angiogenic-properties-of-human-amniotic-epithelial-and-mesenchymal-stem-cells-and-their-effects-on-endothelial-cells
#11
Qianqian Wu, Tao Fang, Hongxin Lang, Min Chen, Ping Shi, Guoxian Qi, Xining Pang
In vivo studies have shown that amnion-produced growth factors participate in many diseases that involve angiogenesis, re-epithelialization and immunomodulation. Although human amniotic epithelial cells (hAECs) and human amniotic mesenchymal stem cells (hAMSCs) can be obtained from amniotic membranes, there is little information regarding their biological differences. The aim of the present study was to isolate and characterize cells from human amnions, to investigate the biological potential and behavior of these cells on the function of endothelial cells in vivo and in vitro and to examine variations in the expression profile of growth factors in different human amnion-derived cell types...
February 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28257830/neuroprotective-effects-of-a-novel-peptide-fk18-under-oxygen-glucose-deprivation-in-sh-sy5y-cells-and-retinal-ischemia-in-rats%C3%A2-via%C3%A2-the-akt-pathway
#12
Shuyu Xiong, Yupeng Xu, Mingming Ma, Haiyan Wang, Fang Wei, Qing Gu, Xun Xu
Ischemic neuronal injury is associated with several life- and vision-threatening diseases. Neuroprotection is essential in the treatment of these diseases. Here, we identified and characterized a novel peptide, FK18, from basic fibroblast growth factor (bFGF). We further assessed the neuroprotective effects of this peptide and its potential mechanisms using the in vitro oxygen-glucose deprivation (OGD) model in SH-SY5Y cells and the in vivo retinal ischemia-reperfusion (I/R) injury model to mimic ischemic neuronal injury...
February 28, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28254316/designer-bfgf-incorporated-d-form-self-assembly-peptide-nanofiber-scaffolds-to-promote-bone-repair
#13
Bin He, Yunsheng Ou, Shuo Chen, Weikang Zhao, Ao Zhou, Jinqiu Zhao, Hong Li, Dianming Jiang, Yong Zhu
d-Form and l-form peptide nanofiber scaffolds can spontaneously form stable β-sheet secondary structures and nanofiber hydrogel scaffolds, and hold some promise in hemostasis and wound healing. We report here on the synthetic self-assembling peptide d-RADA16 and l-RADA16 are both found to produce stable β-sheet secondary structure and nanofiber hydrogel scaffolds based on circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM) and rheology analysis etc. d-RADA16 hydrogel and l-RADA16 hydrogel can enhance obvious bone repair in femoral condyle defects of the Sprague-Dawley (SD) rat model compared to PBS treatment...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28245383/-effect-of-ifn-%C3%AE-on-cytokines-in-serum-of-patients-with-chronic-myeloid-leukemia
#14
Yi Wu, Zhi Cheng, Lin Shi, Lei Feng, Ying-Me Li, Dian-Liang Lyu
OBJECTIVE: To investigate the effect of IFN-α on cytokines in serum of patients with chronic myeloid leukemia(CML). METHODS: Fifty patients with CML from March 2012 to December 2015 in our hospital were randomly divided into routine treatment group (n=25) and combined treatment group (n=25), 30 healthy persons were selected as control (control group). The CML patients in routine treatment group were given orally hydroxyurea, the CML patients in combined treatment group were treated with recombinant human interferon α2b injection based on routine treatment (hydroxyurea plus IFN-α group)...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28244027/different-culture-conditions-affect-the-growth-of-human-tendon-stem-progenitor-cells-tspcs-within-a-mixed-tendon-cells-tcs-population
#15
M Viganò, C Perucca Orfei, A Colombini, D Stanco, P Randelli, V Sansone, L de Girolamo
BACKGROUND: Tendon resident cells (TCs) are a mixed population made of terminally differentiated tenocytes and tendon stem/progenitor cells (TSPCs). Since the enrichment of progenitors proportion could enhance the effectiveness of treatments based on these cell populations, the interest on the effect of culture conditions on the TSPCs is growing. In this study the clonal selection and the culture in presence or absence of basic fibroblast growth factor (bFGF) were used to assess their influences on the stemness properties and phenotype specific features of tendon cells...
December 2017: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/28240438/feeder-independent-canine-induced-pluripotent-stem-cells-maintained-under-serum-free-conditions
#16
Toshiya Nishimura, Shingo Hatoya, Ryoji Kanegi, Daluthgamage Pasty Himali Wijesekera, Kousuke Sanno, Erina Tanaka, Kikuya Sugiura, Noritoshi Kawate, Hiromitsu Tamada, Hiroshi Imai, Toshio Inaba
Canine induced pluripotent stem cells (ciPSCs) are an attractive source for regenerative veterinary medicine, and may also serve as a disease model for human regenerative medicine. Extending the application of ciPSCs from bench to bedside, however, requires the resolution of many issues. We generated ciPSCs expressing doxycycline-inducible murine Oct3/4 (Pou5f1), Sox2, Klf4, and c-Myc, which were introduced using lentiviral vectors. The resultant ciPSCs required doxycycline to proliferate in the undifferentiated state...
February 27, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28237347/generation-of-lif-independent-induced-pluripotent-stem-cells-from-canine-fetal-fibroblasts
#17
N J N Gonçalves, F F Bressan, K C S Roballo, F V Meirelles, P L P Xavier, H Fukumasu, C Williams, M Breen, S Koh, R Sper, J Piedrahita, C E Ambrósio
Takahashi and Yamanaka established the first technique in which transcription factors related to pluripotency are incorporated into the genome of somatic cells to enable reprogramming of these cells. The expression of these transcription factors enables a differentiated somatic cell to reverse its phenotype to an embryonic state, generating induced pluripotent stem cells (iPSCs). iPSCs from canine fetal fibroblasts were produced through lentiviral polycistronic human and mouse vectors (hOSKM/mOSKM), aiming to obtain pluripotent stem cells with similar features to embryonic stem cells (ESC) in this animal model...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28219779/correlations-of-egf-g1380a-bfgf-c754g-and-vegf-t460c-polymorphisms-with-malignant-melanoma-susceptibility-and-prognosis-a-case-control-study
#18
Xin-Hua Wang, Zi-Wen Long
This case-control study aims to investigate the correlations of EGF G1380A, bFGF C754G and VEGF T460C polymorphisms with the susceptibility and prognosis of malignant melanoma. A total of 153 patients with multiple primary melanomas were collected as the case group and another 170 healthy individuals were selected as the control group. ELISA and PCR-RFLP were performed to test the serum level of VEGF and to analyze the genotype as well as allele frequencies of VEGF T460C, EGF G1380A, and bFGF C754G, respectively...
February 17, 2017: Gene
https://www.readbyqxmd.com/read/28217097/feedback-activation-of-basic-fibroblast-growth-factor-signaling-via-the-wnt-%C3%AE-catenin-pathway-in-skin-fibroblasts
#19
Xu Wang, Yuting Zhu, Congcong Sun, Tao Wang, Yingjie Shen, Wanhui Cai, Jia Sun, Lisha Chi, Haijun Wang, Na Song, Chao Niu, Jiayi Shen, Weitao Cong, Zhongxin Zhu, Yuanhu Xuan, Xiaokun Li, Litai Jin
Skin wound healing is a complex process requiring the coordinated behavior of many cell types, especially in the proliferation and migration of fibroblasts. Basic fibroblast growth factor (bFGF) is a member of the FGF family that promotes fibroblast migration, but the underlying molecular mechanism remains elusive. The present RNA sequencing study showed that the expression levels of several canonical Wnt pathway genes, including Wnt2b, Wnt3, Wnt11, T-cell factor 7 (TCF7), and Frizzled 8 (FZD8) were modified by bFGF stimulation in fibroblasts...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28215675/basic-fibroblast-growth-factor-is-critical-to-reprogramming-buffalo-bubalus-bubalis-primordial-germ-cells-into-embryonic-germ-stem-cell-like-cells
#20
Caizhu Wang, Yanfei Deng, Feng Chen, Peng Zhu, Jingwei Wei, Chan Luo, Fenghua Lu, Sufang Yang, Deshun Shi
Primordial germ cells (PGCs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (EG) cells in vitro. Buffalo PGC have been reported to be reprogrammed into EG-like cells, but the identities of the major signaling pathways and culture media involved in this derivation remain unclear. Here, the effects of basic fibroblast growth factor (bFGF) and downstream signaling pathways on the reprogramming of buffalo PGCs into EG-like cells were investigated. Results showed bFGF to be critical to buffalo PGCs to dedifferentiate into EG-like cells (20 ng/mL is optimal) with many characteristics of pluripotent stem cells, including alkaline phosphatase (AP) activity, expression of pluripotency marker genes such as OCT4, NANOG, SOX2, SSEA-1, CDH1, and TRA-1-81, and the capacity to differentiate into all three embryonic germ layers...
March 15, 2017: Theriogenology
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