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https://www.readbyqxmd.com/read/28915498/tantalum-coated-pedicle-screws-enhance-implant-integration
#1
Liang-Yu Shi, An Wang, Fa-Zhi Zang, Jian-Xi Wang, Xian-Wei Pan, Hua-Jiang Chen
Because titanium alloy (Ti) has the natural advantage of a low elastic modulus, it has become the most commonly used material for the manufacturing of pedicle screws. However, its poor shear strength and osteogenic ability are undesirable properties. The superior osteoinductivity demonstrated by tantalum (Ta) in oral and maxillofacial surgery and joint surgery leads us to assume that the tantalum-coated pedicle screws may have better osteogenic properties and bone anchoring strength. To verify this hypothesis, MC3T3-E1 cells and human mesenchymal stem cells (hBMSCs) were seeded on the surface of Ta and Ti disks to compare the effects of two different metals on cell adhesion, proliferation, and differentiation...
September 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28887826/job-contenders-roles-of-the-%C3%AE-barrel-assembly-machinery-and-the-translocation-and-assembly-module-in-autotransporter-secretion
#2
REVIEW
Cécile Albenne, Raffaele Ieva
In Gram-negative bacteria, autotransporters secrete effector protein domains that are linked to virulence. Although they were once thought to be simple and autonomous secretion machines, mounting evidence reveals that multiple factors of the bacterial envelope are necessary for autotransporter assembly. Secretion across the outer membrane of their soluble effector "passenger domain" is promoted by the assembly of an outer membrane-spanning "β-barrel domain". Both reactions require BamA, an essential component of the β-barrel assembly machinery (BAM complex) that catalyzes the final reaction step by which outer membrane proteins are integrated into the lipid bilayer...
September 8, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28886680/outer-membrane-biogenesis
#3
Anna Konovalova, Daniel E Kahne, Thomas J Silhavy
The hallmark of gram-negative bacteria and organelles such as mitochondria and chloroplasts is the presence of an outer membrane. In bacteria such as Escherichia coli, the outer membrane is a unique asymmetric lipid bilayer with lipopolysaccharide in the outer leaflet. Integral transmembrane proteins assume a β-barrel structure, and their assembly is catalyzed by the heteropentameric Bam complex containing the outer membrane protein BamA and four lipoproteins, BamB-E. How the Bam complex assembles a great diversity of outer membrane proteins into a membrane without an obvious energy source is a particularly challenging problem, because folding intermediates are predicted to be unstable in either an aqueous or a hydrophobic environment...
September 8, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28866660/generation-of-tryptophan-hydroxylase-2-gene-knockout-pigs-by-crispr-cas9-mediated-gene-targeting
#4
Ze Li, Hai-Yuan Yang, Ying Wang, Man-Ling Zhang, Xiao-Rui Liu, Qiang Xiong, Li-Ning Zhang, Yong Jin, Li-Sha Mou, Yan Liu, Rong-Feng Li, Yi Rao, Yi-Fan Dai
Unbalanced brain serotonin (5-HT) levels have implications in various behavioral abnormalities and neuropsychiatric disorders. The biosynthesis of neuronal 5-HT is regulated by the rate-limiting enzyme, tryptophan hydroxylase-2 (TPH2). In the present study, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system was used to target the Tph2 gene in Bama mini pig fetal fibroblasts. It was found that CRISPR/Cas9 targeting efficiency could be as high as 61.5%, and the biallelic mutation efficiency reached at 38...
September 3, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28790934/skin-lesions-in-swine-with-decompression-sickness-clinical-appearance-and-pathogenesis
#5
Long Qing, Dinesh K Ariyadewa, Hongjie Yi, Yewei Wang, Quan Zhou, Weigang Xu
Skin lesions are visual clinical manifestations of decompression sickness (DCS). Comprehensive knowledge of skin lesions would give simple but strong clinical evidence to help diagnose DCS. The aim of this study was to systematically depict skin lesions and explore their pathophysiological basis in a swine DCS model. Thirteen Bama swine underwent simulated diving in a hyperbaric animal chamber with the profile of 40 msw-35 min exposure, followed by decompression in 11 min. After decompression, chronological changes in the appearance of skin lesions, skin ultrasound, temperature, tissue nitric oxide (NO) levels, and histopathology were studied...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28760846/conformational-changes-that-coordinate-the-activity-of-bama-and-bamd-allowing-%C3%AE-barrel-assembly
#6
Anne L McCabe, Dante Ricci, Modupe Adetunji, Thomas J Silhavy
Most integral outer membrane proteins (OMPs) of Gram-negative bacteria such as Escherichia coli assume a β-barrel structure. The β-barrel Assembly Machine (Bam)-a five-member complex composed of β-barrel OMP BamA and four associated lipoproteins, BamB,C,D, and E-folds and inserts OMPs into the outer membrane. The two essential proteins, BamA and BamD interact to stabilize two subcomplexes, BamAB and BamCDE and genetic and structural evidence suggests that interactions between BamA and BamD occur via an electrostatic interaction between a conserved aspartate residue in a periplasmic domain of BamA and a conserved arginine in BamD...
July 31, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28760137/prevalence-and-clinical-profile-of-metabolic-syndrome-in-longevity-study-from-guangxi-zhuang-autonomous-region-china
#7
Xianghua He, Wei Zhang, Guofang Pang, Yuan Lv, Caiyou Hu, Ze Yang
BACKGROUND: Metabolic syndrome (MetS) was a risk factor for cardiovascular diseases, yet the prevalence of MetS among nonagenarians and centenarians was rarely reported. Here we investigated the prevalence of MetS and its components among nonagenarians and centenarians in our Zhuang population from Bama, Guangxi Zhuang Autonomous Region, China. METHOD: In Bama area, there registered 881 individuals who lived more than 90 years old in 269,800 local residents and our study involved 307 long-lived participants and 486 local younger (35-68 years) persons, as controls...
July 31, 2017: BMC Geriatrics
https://www.readbyqxmd.com/read/28756900/evaluation-of-early-kidney-damage-caused-by-brain-death-using-real-time-ultrasound-elastography-in-a-bama-pig-model
#8
Ying Tang, Jingwen Zhao, Dongyang Liu, Ningning Niu, Huimin Yu
The aim of this study was to investigate the value of real-time tissue elastography (RTE) in the evaluation of early graft damage resulting from brain death. We performed RTE before and 0, 3, 6 and 9 h after brain death in a Bama pig model. Eleven RTE parameters were compared among time groups, and their correlations with electron microscopic findings were analyzed. Receiver operating characteristic curve analysis was used to find the RTE parameter cutoff values. The mean relative strain value within the region of interest (MEAN), standard deviation of the relative strain value within the region of interest (SD), percentage area of low strain within the region of interest (%AREA), complexity of low-strain area within the region of interest (COMP), kurtosis (KURT), skewness (SKEW), contrast (CONT) and entropy (ENT) and inverse difference moment (IDM) differed statistically significantly between groups (p < 0...
July 27, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28752534/structural-basis-for-substrate-selection-by-the-translocation-and-assembly-module-of-the-%C3%AE-barrel-assembly-machinery
#9
Rebecca S Bamert, Karl Lundquist, Hyea Hwang, Chaille T Webb, Takoya Shiota, Christopher J Stubenrauch, Mathew J Belousoff, Robert J A Goode, Ralf B Schittenhelm, Richard Zimmerman, Martin Jung, James C Gumbart, Trevor Lithgow
The assembly of proteins into bacterial outer membranes is a key cellular process that we are only beginning to understand, mediated by the β-barrel assembly machinery (BAM). Two crucial elements of that machinery are the core BAM complex and the translocation and assembly module (TAM), with each containing a member of the Omp85 superfamily of proteins: BamA in the BAM complex, TamA in the TAM. Here, we used the substrate protein FimD as a model to assess the selectivity of substrate interactions for the TAM relative to those of the BAM complex...
July 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28715930/transcription-analysis-of-cochlear-development-in-minipigs
#10
Qinghua Wang, Yan Zhang, Weiwei Guo, Yu Liu, Hong Wei, Shiming Yang
CONCLUSIONS: The critical stage for cochlear gene regulation in the embryo is E49-E56. These data provide a valuable resource to help elucidate gene regulation in cochlear development and will benefit ear-related studies, especially those that focus on gene and stem cell therapies in hearing loss. OBJECTIVES: To investigate gene regulation in cochlear development and provide reference information for ear-related research. METHODS: Total RNA extraction from the Bama miniature pig cochlea was performed at five time points that covered most of the cochlea development process (E42, E49, E56, E91, P13)...
July 18, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28713344/outer-membrane-proteome-of-veillonella-parvula-a-diderm-firmicute-of-the-human-microbiome
#11
Daniel I Poppleton, Magalie Duchateau, Véronique Hourdel, Mariette Matondo, Jennifer Flechsler, Andreas Klingl, Christophe Beloin, Simonetta Gribaldo
Veillonella parvula is a biofilm-forming commensal found in the lungs, vagina, mouth, and gastro-intestinal tract of humans, yet it may develop into an opportunistic pathogen. Furthermore, the presence of Veillonella has been associated with the development of a healthy immune system in infants. Veillonella belongs to the Negativicutes, a diverse clade of bacteria that represent an evolutionary enigma: they phylogenetically belong to Gram-positive (monoderm) Firmicutes yet maintain an outer membrane (OM) with lipopolysaccharide similar to classic Gram-negative (diderm) bacteria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28702706/metagenomic-analysis-of-subtidal-sediments-from-polar-and-subpolar-coastal-environments-highlights-the-relevance-of-anaerobic-hydrocarbon-degradation-processes
#12
Fernando Espínola, Hebe M Dionisi, Sharon Borglin, Colin J Brislawn, Janet K Jansson, Walter P Mac Cormack, JoLynn Carroll, Sara Sjöling, Mariana Lozada
In this work, we analyzed the community structure and metabolic potential of sediment microbial communities in high-latitude coastal environments subjected to low to moderate levels of chronic pollution. Subtidal sediments from four low-energy inlets located in polar and subpolar regions from both Hemispheres were analyzed using large-scale 16S rRNA gene and metagenomic sequencing. Communities showed high diversity (Shannon's index 6.8 to 10.2), with distinct phylogenetic structures (<40% shared taxa at the Phylum level among regions) but similar metabolic potential in terms of sequences assigned to KOs...
July 12, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28683436/effects-of-trimetazidine-on-pdcd4-nf-%C3%AE%C2%BAb-tnf-%C3%AE-pathway-in-coronary-microembolization
#13
Qiang Su, Lang Li, Jinmin Zhao, Yuhan Sun, Huafeng Yang
BACKGROUND/AIMS: The local inflammatory response caused by coronary microembolization (CME) is the primary cause of progressive cardiac dysfunction. The PDCD4/NF-κB/TNF-α signaling pathway plays a significant role in CME-induced myocardial Inflammation. Trimetazidine (TMZ) reduces myocardial injury, caused by percutaneous coronary intervention, through relieving the CME-induced myocardial systolic dysfunction. Therefore, the present study investigated the role of TMZ pre-treatment in the protection of myocardium after CME and PDCD4/NF-κB/TNF-α in mini pigs...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28674285/a-high-sucrose-and-high-fat-diet-induced-the-development-of-insulin-resistance-in-the-skeletal-muscle-of-bama-miniature-pigs-through-the-akt-glut4-pathway
#14
Yaqian Liu, Jifang Yuan, Lei Xiang, Yuqiong Zhao, Miaomiao Niu, Xin Dai, Hua Chen
A high sucrose and high fat (HSHF) diet induces insulin resistance (IR) and increased susceptibility to type 2 diabetes mellitus (T2DM), but the underlying mechanisms are poorly characterized. This study aimed to investigate the molecular mechanisms by which the HSHF diet impairs insulin sensitivity in Bama miniature pigs (sus scrofa domesticus). Twelve Bama miniature pigs were randomly assigned to the control diet (CD) group (n=6) or the HSHF group (n=6) for 6 months. Biochemical parameters were measured. Western blot, RT-qPCR and immunohistochemistry were used to profile the changes of protein expression, mRNA expression and glucose transporter 4 (GLUT4) expression in skeletal muscle tissues, respectively...
July 3, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28672963/easapprox-%C3%A2-skin-stretching-system-a-secure-and-effective-method-to-achieve-wound-closure
#15
Mingzhi Song, Zhen Zhang, Tao Liu, Song Liu, Gang Li, Zhaochang Liu, Jingyang Huang, Song Chen, Linan Li, Li Guo, Yang Qiu, Jiajia Wan, Yuejian Liu, Tao Wu, Xiaoyong Wang, Ming Lu, Shouyu Wang
Large skin defects are commonly observed in the clinic and have attracted much attention recently. Therefore, finding an effective solution for large skin defects is a global problem. The objective of the present study was to assess the effectiveness of the EASApprox(®) skin-stretching system for closing large skin defects. Skin defects (5×5 cm) were created on the forearms of 9 Bama miniature pigs, which were randomly divided into the following three groups: Direct suture, the new EASApprox(®) skin-stretching device and Kirschner wires...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28630161/insertion-of-proteins-and-lipopolysaccharide-into-the-bacterial-outer-membrane
#16
REVIEW
Istvan Botos, Nicholas Noinaj, Susan K Buchanan
The bacterial outer membrane contains phospholipids in the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet. Both proteins and LPS must be frequently inserted into the outer membrane to preserve its integrity. The protein complex that inserts LPS into the outer membrane is called LptDE, and consists of an integral membrane protein, LptD, with a separate globular lipoprotein, LptE, inserted in the barrel lumen. The protein complex that inserts newly synthesized outer-membrane proteins (OMPs) into the outer membrane is called the BAM complex, and consists of an integral membrane protein, BamA, plus four lipoproteins, BamB, C, D and E...
August 5, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28616917/-a-study-on-the-feasibility-to-assay-minipig-insulin-through-human-insulin-radioimmunoassay-kit
#17
Meng-Yu Yang, Yun-Yi Gao, Yan Li, Ya-Li Li, Hao Zhong, Xing-Wu Ran
OBJECTIVES: To investigate the feasibility of using human-specific insulin radioimmunoassay (Ins-RIA) kit to measure the concentrations of serum insulin in minipigs. METHODS: The Serum samples (219) of Bama minipigs were collected. The concentrations of serum insulin in Bama minipigs were measured by Ins-RIA kit and porcine-specific insulin enzyme linked immunosorbent assay (Ins-ELISA) kit, respectively. The linear regression analysis was undertaken in a randomly selected 175 samples (80% of total samples) to establish predictive equations between the concentrations of serum insulin measured by Ins-ELISA and the concentrations of serum insulin measured by Ins-RIA...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28573043/a-safety-evaluation-of-profound-hypothermia-induced-suspended-animation-for-delayed-resuscitation-at-90-or-120%C3%A2-min
#18
Yu Liu, Shu Li, Zhi Li, Jian Zhang, Jin-Song Han, Yong Zhang, Zong-Tao Yin, Hui-Shan Wang
BACKGROUND: The successful treatment of military combat casualties with penetrating injuries is significantly dependent on the time needed to get the patient to an adequate treatment facility. Profound hypothermia-induced suspended animation for delayed resuscitation (SADR) is a novel approach for inducing cardiac arrest and buying additional time for such injuries. However, the time used to safely administer circulatory arrest (CA) is controversial. The goal of this study was to evaluate the safety of hypothermia-induced SADR over 90 and 120 min time intervals...
2017: Military Medical Research
https://www.readbyqxmd.com/read/28569500/extreme-dynamics-in-the-bama-%C3%AE-barrel-seam
#19
Pamela Arden Doerner, Marcelo C Sousa
BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular mechanism remains poorly understood. Crystal structures of BamA and complementary molecular dynamics simulations have shown that its β-barrel seam (the interface between the first and last barrel strands) is destabilized...
June 12, 2017: Biochemistry
https://www.readbyqxmd.com/read/28558932/bama-miniature-pigs-liver-possess-great-heat-tolerance-through-upregulation-of-nrf2-mediated-antioxidative-enzymes
#20
Jiaqin Chen, Fei Wang, Xin Zhou, Yun Cao, Yansen Li, Chunmei Li
The liver is one of the most crucial organs affected by high ambient temperature. Bama miniature pig show a heat tolerance in hot summer months. However, the physiological condition of liver under high ambient temperature has not been well elucidated in Bama miniature pig. Here we performed an experiment to investigate the effects of high ambient temperature on liver function, redox status and Nrf2 antioxidant pathway in Bama miniature pigs. Twelve pigs were randomly divided into two groups and separately exposed to the neutral temperature (NT, 25°C) and high temperature (HT, 40°C) for 8 days...
July 2017: Journal of Thermal Biology
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