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https://www.readbyqxmd.com/read/28174029/effect-of-dietary-zinc-on-morphological-characteristics-and-apoptosis-related-gene-expression-in-the-small-intestine-of-bama-miniature-pigs
#1
Xin Zhou, Yansen Li, Zhaojian Li, Yun Cao, Fei Wang, ChunMei Li
To investigate the effects of dietary zinc (Zn) on small intestinal mucosal epithelium, 6-month-old male Bama miniature pigs were randomly allocated into three groups and treated with three levels of Zn (Control, T1, and T2 diet supplemented with 0, 50, and 1500mg/kg Zn, respectively, as zinc sulfate) for 38days. The samples of small intestine tissues, serum, and feces were collected. The results showed that Zn concentrations of small intestine in the T2 group were higher than those in the control and T1 groups (p<0...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28166946/preventive-effects-of-supplemental-dietary-zinc-on-heat-induced-damage-in-the-epididymis-of-boars
#2
Zhaojian Li, Yansen Li, Xin Zhou, Yun Cao, Chunmei Li
Hyperthermia in boars reduces growth performance and sperm production. Zinc is an essential trace element in animal nutrition. Here we investigate the effects of dietary zinc on epididymal structure and function in Bama miniature pigs treated with heat exposure and investigate approaches to improve the reproductive performance in summer. Male Bama miniature pigs (n=18; aged 6 months; bodyweight=10.79±0.06kg) were randomly allocated to 3 groups: control group (Control), heat treatment group (HT), and the diet-supplemented and heat treatment group (H+Zn)...
February 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28027289/paraoxonase-1-pon1-rs662-polymorphism-and-its-association-with-serum-lipid-levels-and-longevity-in-the-bama-zhuang-population
#3
You Li, Guiyun Liang, Liwei Shi, Xue Liang, Bingshuang Long, Jian Qin, Zhiyong Zhang
BACKGROUND The present study was performed to identify the association of PON1 rs662 polymorphism with serum lipid levels and human longevity in the Bama Zhuang population. MATERIAL AND METHODS PON1 genotypes were determined by Taqman SNP Genotyping Assays in 110 long-lived inhabitants (longevity group, aged 90-110 years), 110 healthy inhabitants in Bama County (control 1 group, aged 43-82 years) and 110 healthy inhabitants in Nandan County (control 2 group, aged 28-82 years) without family history of longevity...
December 27, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27989620/flexibility-in-the-periplasmic-domain-of-bama-is-important-for-function
#4
Lisa R Warner, Petia Z Gatzeva-Topalova, Pamela A Doerner, Arthur Pardi, Marcelo C Sousa
The β-barrel assembly machine (BAM) mediates the biogenesis of outer membrane proteins (OMPs) in Gram-negative bacteria. BamA, the central BAM subunit composed of a transmembrane β-barrel domain linked to five polypeptide transport-associated (POTRA) periplasmic domains, is thought to bind nascent OMPs and undergo conformational cycling to catalyze OMP folding and insertion. One model is that conformational flexibility between POTRA domains is part of this conformational cycling. Nuclear magnetic resonance (NMR) spectroscopy was used here to study the flexibility of the POTRA domains 1-5 in solution...
January 3, 2017: Structure
https://www.readbyqxmd.com/read/27982054/mitochondrial-bacterial-hybrids-of-bama-tob55-suggest-variable-requirements-for-the-membrane-integration-of-%C3%AE-barrel-proteins
#5
Anna-Katharina Pfitzner, Nadja Steblau, Thomas Ulrich, Philipp Oberhettinger, Ingo B Autenrieth, Monika Schütz, Doron Rapaport
β-Barrel proteins are found in the outer membrane (OM) of Gram-negative bacteria, chloroplasts and mitochondria. The assembly of these proteins into the corresponding OM is facilitated by a dedicated protein complex that contains a central conserved β-barrel protein termed BamA in bacteria and Tob55/Sam50 in mitochondria. BamA and Tob55 consist of a membrane-integral C-terminal domain that forms a β-barrel pore and a soluble N-terminal portion comprised of one (in Tob55) or five (in BamA) polypeptide transport-associated (POTRA) domains...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894390/-effects-of-different-fluid-resuscitation-program-on-renal-function-in-swine-during-shock-stage-of-severe-burn
#6
G L Su, W X Huang, J Chen, D J Xue, J J Zhou
Objective: To explore the effects of different fluid resuscitation program on renal function in swine during shock stage of severe burn. Methods: Twenty-four Guangxi Bama miniature swine were inflicted with 40% total body surface area on the back, and then they were divided into four groups according to the random number table, with 6 swine in each group. At post injury hour (PIH) 2, swine in succinylated gelatin group (S), hydroxyethyl starch group (H), and allogeneic plasma group (A) were respectively resuscitated with succinylated gelatin, hydroxyethyl starch 130/0...
November 20, 2016: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/27866536/-in-vivo-and-in-vitro-experimental-studies-of-remote-digital-control-endovascular-robotic-system-in-interventional-angiography
#7
J Y Tan, L Q Jia, W H Shi, Z Y Tian, J D Tong, Y Deng, B Yu
Objective: To investigate the feasibility of the remote control endovascular robotic system in interventional angiography. Method: The endovascular robotic system Heifetz™ and the matched steerable catheter Mirage™ were used to cannulate all the target vessels in the vascular model and cerebral arteries originated from the aorta of Bama mini-pigs under fluoroscopic guidance. The technical success rate, cannulation time and exposure dose were all collected and compared with the result of using conventional techniques...
November 15, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27852910/intraosseous-injection-of-simvastatin-in-poloxamer-407-hydrogel-improves-pedicle-screw-fixation-in-ovariectomized-minipigs
#8
X Fu, J Tan, C G Sun, H J Leng, Y S Xu, C L Song
BACKGROUND: Osteoporosis leads to poor osseointegration and reduces implant stability. Statins have been found to stimulate bone formation, but the bioavailability from oral administration is low. Local application may be more effective at augmenting bone formation and enhancing implant stability. This study was performed to evaluate the efficacy of an intraosseous injection of simvastatin in thermosensitive poloxamer 407 hydrogel to enhance pedicle-screw fixation in calcium-restricted ovariectomized minipigs...
November 16, 2016: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/27806730/comparative-physiological-plasticity-to-desiccation-in-distinct-populations-of-the-malarial-mosquito-anopheles-coluzzii
#9
K Hidalgo, D Siaussat, V Braman, K R Dabiré, F Simard, K Mouline, D Renault
BACKGROUND: In West Africa, populations of the malaria vector mosquito, Anopheles coluzzii, are seasonally exposed to strong desiccating conditions during the dry season. Their dynamics strictly follows the pace of the availability of suitable larval development sites (water collections). Accordingly, mosquitoes can reproduce all year long where permanent breeding is possible, or stop reproduction and virtually disappear at the onset of the dry season when surface water dries up, like observed in temporary habitats of dry savannah areas...
November 2, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27686148/lateral-opening-in-the-intact-%C3%AE-barrel-assembly-machinery-captured-by-cryo-em
#10
Matthew G Iadanza, Anna J Higgins, Bob Schiffrin, Antonio N Calabrese, David J Brockwell, Alison E Ashcroft, Sheena E Radford, Neil A Ranson
The β-barrel assembly machinery (BAM) is a ∼203 kDa complex of five proteins (BamA-E), which is essential for viability in E. coli. BAM promotes the folding and insertion of β-barrel proteins into the outer membrane via a poorly understood mechanism. Several current models suggest that BAM functions through a 'lateral gating' motion of the β-barrel of BamA. Here we present a cryo-EM structure of the BamABCDE complex, at 4.9 Å resolution. The structure is in a laterally open conformation showing that gating is independent of BamB binding...
2016: Nature Communications
https://www.readbyqxmd.com/read/27650471/distension-of-the-toga-of-thermotoga-maritima-involves-continued-growth-of-the-outer-envelope-as-cells-enter-the-stationary-phase
#11
Chaman Ranjit, Kenneth M Noll
Thermotoga maritima cells are distinguished by a sheath-like structure called the toga that loosely encloses single or multiple cells. During growth, and particularly at late phases of population growth, the toga distends from the poles of many cells. Little is known about this phenomenon so this study presents basic information about this process. We first provide quantitative data demonstrating that cells showing toga distensions increase in number during growth and that the phenomenon is not due to acidification of their growth medium...
October 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27603665/a-translational-study-of-a-new-therapeutic-approach-for-acute-myocardial-infarction-nanoparticle-mediated-delivery-of-pitavastatin-into-reperfused-myocardium-reduces-ischemia-reperfusion-injury-in-a-preclinical-porcine-model
#12
Kenzo Ichimura, Tetsuya Matoba, Kaku Nakano, Masaki Tokutome, Katsuya Honda, Jun-Ichiro Koga, Kensuke Egashira
BACKGROUND: There is an unmet need to develop an innovative cardioprotective modality for acute myocardial infarction, for which interventional reperfusion therapy is hampered by ischemia-reperfusion (IR) injury. We recently reported that bioabsorbable poly(lactic acid/glycolic acid) (PLGA) nanoparticle-mediated treatment with pitavastatin (pitavastatin-NP) exerts a cardioprotective effect in a rat IR injury model by activating the PI3K-Akt pathway and inhibiting inflammation. To obtain preclinical proof-of-concept evidence, in this study, we examined the effect of pitavastatin-NP on myocardial IR injury in conscious and anesthetized pig models...
2016: PloS One
https://www.readbyqxmd.com/read/27596934/comparison-of-methods-for-isolating-primary-hepatocytes-from-mini-pigs
#13
Yi Li, Yujia Wang, Qiong Wu, Li Li, Yujun Shi, Hong Bu, Ji Bao
Successful porcine hepatocyte isolation is crucial for the development of bioartificial liver devices and hepatocyte transplantation. Serva collagenase NB grades are formulated collagenases that are suitable for various tissue isolation applications. N-acetylcysteine (NAC) can improve the viability of human hepatocytes. The aim of this study was to compare the effectiveness of two collagenases and effect of NAC on hepatocyte isolation from porcine liver tissue. Porcine hepatocytes were isolated using the perfusion method from Bama mini pigs assigned to the Serva NB 4 group (n=6), the Serva NB 8 group (n=6), or the NB 8+NAC group (n=6)...
September 2016: Xenotransplantation
https://www.readbyqxmd.com/read/27588694/the-tamb-ortholog-of-borrelia-burgdorferi-interacts-with-the-%C3%AE-barrel-assembly-machine-bam-complex-protein-bama
#14
Henna Iqbal, Melisha R Kenedy, Meghan Lybecker, Darrin R Akins
Two outer membrane protein (OMP) transport systems in diderm bacteria assist in assembly and export of OMPs. These two systems are the β-barrel assembly machine (BAM) complex and the translocation and assembly module (TAM). The BAM complex consists of the OMP component BamA along with several outer membrane associated proteins. The TAM also consists of an OMP, designated TamA, and a single inner membrane (IM) protein, TamB. Together TamA and TamB aid in the secretion of virulence-associated OMPs. In this study we characterized the hypothetical protein BB0794 in Borrelia burgdorferi...
December 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27572508/intestinal-lactobacillus-community-structure-and-its-correlation-with-diet-of-southern-chinese-elderly-subjects
#15
Yuanyuan Pan, Fang Wang, Da-Wen Sun, Quanyang Li
This study aimed to investigate the relationship between the intestinal Lactobacillus species and diet of elderly subjects in a longevity area in Southern China. Healthy elderly subjects ranging from 80 to 99 years old were respectively selected from the regions of Bama and Nanning, Guangxi, China. The nested polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) technology was used to analyze the intestinal Lactobacillus community structure. Results showed that Weissella confusa, L...
September 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27534702/the-bama-miniature-swine-is-susceptible-to-experimental-hev-infection
#16
Zi-Min Tang, Si-Ling Wang, Dong Ying, Gui-Ping Wen, Wei Cai, Ke Zhang, Wen-Fang Ji, Ming Yang, Zi-Zheng Zheng, Ning-Shao Xia
The hepatitis E virus (HEV) is one of the main causes of enterically transmitted hepatitis worldwide. Although the mortality rates associated with HEV are generally low, they can be up to 28% in HEV-infected pregnant women, and the elderly are more susceptible. The reasons for this selective severity are unclear, partially because there is no suitable, easy-to-use model in which to study HEV infection. Non-human primates and standard swine have been identified as being sensitive to infection with HEV and have been used for HEV infection studies...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27525927/isolation-proliferation-and-induction-of-bama-mini-pig-spermatogonial-stem-cells-in-vitro
#17
H M Zhao, H Yang, F H Luo, M X Li, S Zhang, X G Yang, Y Q Lu, S S Lu, Y J Wu, K H Lu
Spermatogonial stem cells (SSCs), the unique seed cells of testes, can undergo meiosis and form spermatozoa, thus transmitting genetic information to offspring. Research concerning these cells explores the mechanism underlying spermatogenesis, making possible the induction of their differentiation into spermatozoa in vitro. SSCs have therefore attracted much interest among scientists. Although the proliferation of such cells in vitro has been demonstrated, we are unaware of any long-term laboratory culture of porcine SSCs...
August 12, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27439868/characterization-of-a-stalled-complex-on-the-%C3%AE-barrel-assembly-machine
#18
James Lee, Mingyu Xue, Joseph S Wzorek, Tao Wu, Marcin Grabowicz, Luisa S Gronenberg, Holly A Sutterlin, Rebecca M Davis, Natividad Ruiz, Thomas J Silhavy, Daniel E Kahne
The assembly of β-barrel proteins into membranes is mediated by an evolutionarily conserved machine. This process is poorly understood because no stable partially folded barrel substrates have been characterized. Here, we slowed the folding of the Escherichia coli β-barrel protein, LptD, with its lipoprotein plug, LptE. We identified a late-stage intermediate in which LptD is folded around LptE, and both components interact with the two essential β-barrel assembly machine (Bam) components, BamA and BamD...
August 2, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27427744/chemiluminescent-detection-for-estimating-relative-copy-numbers-of-porcine-endogenous-retrovirus-proviruses-from-chinese-minipigs-based-on-magnetic-nanoparticles
#19
Haowen Yang, Ming Liu, Bingcong Zhou, Yan Deng, Nongyue He, Hesheng Jiang, Yafen Guo, Ganqiu Lan, Qinyang Jiang, Xiurong Yang, Zhiyang Li
Chinese Bama minipigs could be potential donors for the supply of xenografts because they are genetically stable, highly inbred, and inexpensive. However, porcine endogenous retrovirus (PERV) is commonly integrated in pig genomes and could cause a cross-species infection by xenotransplantation. For screening out the pigs with low copy numbers of PERV proviruses, we have developed a novel semiquantitative analysis approach based on magnetic nanoparticles (MNPs) and chemiluminescence (CL) for estimating relative copy numbers (RCNs) of PERV proviruses in Chinese Bama minipigs...
June 2016: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/27421023/comparisons-of-different-myosin-heavy-chain-types-ampk-and-pgc-1%C3%AE-gene-expression-in-the-longissimus-dorsi-muscles-in-bama-xiang-and-landrace-pigs
#20
Y N Huang, Q W Ao, Q Y Jiang, Y F Guo, G Q Lan, H S Jiang
Bama Xiang and Landrace pigs are the local fatty and lean breeds, respectively, in China. We compared differences in carcass traits, meat quality traits, and myosin heavy chain (MyHC) types in the longissimus dorsi muscles between Bama Xiang and Landrace pigs. This was done in pigs of the same age, using real-time PCR, to investigate the relationship between MyHC fiber types and carcass characteristics, meat quality traits, and the key factors regulating muscle fiber type. Bama Xiang pigs exhibited smaller size and slower growth than Landrace pigs (P < 0...
July 14, 2016: Genetics and Molecular Research: GMR
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