keyword
https://read.qxmd.com/read/38279484/regulatory-mechanism-of-interaction-between-y-box-binding-protein-1-and-heterogenous-nuclear-ribonucleoprotein-k-in-cell-division-cycle-25a-signal-pathway-and-lung-cancer-metastasis
#1
JOURNAL ARTICLE
Lvping Fu, Mengmeng Yang, Yuquan Ma
The research aimed to the influences of the interaction between Y-box-binding protein 1 (YBX1) and heterogeneous nuclear ribonucleoprotein K (HNRNPK) on cell division cycle protein 25 phosphatase A (CDC25a) signal pathway and the regulatory mechanism of lung cancer (LC) metastasis. A total of 34 patients diagnosed with LC pathologically were selected as the research objects, and the expression levels of YBX1, HNRNP and CDC25a in LC non-metastasis tissues and LC metastasis tissues were detected by immunohistochemistry and Western blot (WB)...
December 20, 2023: Cellular and Molecular Biology
https://read.qxmd.com/read/38235706/switchable-tetraplex-elements-in-the-heterogeneous-nuclear-ribonucleoprotein-k-promoter-micro-environment-dictated-structural-transitions-of-g-c-rich-elements
#2
JOURNAL ARTICLE
Debopriya Bose, Nilanjan Banerjee, Ananya Roy, Pallabi Sengupta, Subhrangsu Chatterjee
We have elucidated the hnRNP K promoter as a hotspot for tetraplex-based molecular switches receptive to micro-environmental stimuli. We have characterised the structural features of four tetraplex-forming loci and identified them as binding sites of transcription factors. These segments form either G-quadruplex or i-motif structures, the structural dynamicity of which has been studied in depth via several biophysical techniques. The tetraplexes display high dynamicity and are influenced by both pH and KCl concentrations in vitro ...
January 18, 2024: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/38008244/insight-into-the-mechanism-of-aml-del-9q-progression-hnrnp-k-targets-the-myeloid-master-regulators-cebpa-c-ebp%C3%AE-and-spi1-pu-1
#3
JOURNAL ARTICLE
Kerstin Rahn, Ali T Abdallah, Lin Gan, Shelley Herbrich, Roland Sonntag, Oscar Benitez, Prerna Malaney, Xiaorui Zhang, Ashely G Rodriguez, Jared Brottem, Gernot Marx, Tim H Brümmendorf, Dirk H Ostareck, Antje Ostareck-Lederer, Martina Crysandt, Sean M Post, Isabel S Naarmann-de Vries
Deletions on the long arm of chromosome 9 (del(9q)) are recurrent abnormalities in about 2 % of acute myeloid leukemia cases, which usually involve HNRNPK and are frequently associated with other known aberrations. Based on an Hnrnpk haploinsufficient mouse model, a recent study demonstrated a function of hnRNP K in pathogenesis of myeloid malignancies via the regulation of cellular proliferation and myeloid differentiation programs. Here, we provide evidence that reduced hnRNP K expression results in the dysregulated expression of C/EBPα and additional transcription factors...
November 24, 2023: Biochimica et Biophysica Acta. Gene Regulatory Mechanisms
https://read.qxmd.com/read/37962331/decoding-complexity-in-biomolecular-recognition-of-dna-i-motifs-with-microarrays
#4
JOURNAL ARTICLE
Kamyar Yazdani, Srinath Seshadri, Desiree Tillo, Mo Yang, Christopher D Sibley, Charles Vinson, John S Schneekloth
DNA i-motifs (iMs) are non-canonical C-rich secondary structures implicated in numerous cellular processes. Though iMs exist throughout the genome, our understanding of iM recognition by proteins or small molecules is limited to a few examples. We designed a DNA microarray containing 10976 genomic iM sequences to examine the binding profiles of four iM-binding proteins, mitoxantrone and the iMab antibody. iMab microarray screens demonstrated that pH 6.5, 5% BSA buffer was optimal, and fluorescence was correlated with iM C-tract length...
November 14, 2023: Nucleic Acids Research
https://read.qxmd.com/read/37780856/the-characterization-of-rna-binding-proteins-and-rna-metabolism-related-proteins-in-fungal-extracellular-vesicles
#5
JOURNAL ARTICLE
Marianna Dallastella, Willian Klassen de Oliveira, Marcio L Rodrigues, Samuel Goldenberg, Lysangela R Alves
RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs...
2023: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/37652868/mex3c-induces-cognitive-impairment-in-mice-through-autophagy-inhibition
#6
JOURNAL ARTICLE
Kai Wang, Hao-Nan Zhang, Yong Du
BACKGROUND: The muscle excess 3 (MEX3C) protein comprises one of two conserved KH hnRNP K homology domains of the Caenorhabditis elegans protein family, a gene involved in the metabolism of key RNAs at posttranscriptional levels during the development of C. elegans, but its function in mammals is unclear. METHODS AND RESULTS: In this study, we found that MEX3C plays a key role in learning and cognitive function. The learning and cognitive abilities of MEX3C-knockout (KO) mice were significantly decreased relative to those of wild-type (WT) mice in behavioral experiments, including the shuttle box, Morris water maze, and new object recognition...
August 31, 2023: Brain and Behavior
https://read.qxmd.com/read/37427379/bioactivity-guided-fractionation-of-helicteres-angustifolia-l-extract-and-its-molecular-evidence-for-tumor-suppression
#7
JOURNAL ARTICLE
Kejuan Li, Shuang Sun, Long Xiao, Zhenya Zhang
Helicteres angustifolia L. ( Helicteres angustifolia ) has been commonly used in folk medicine to treat cancer; however, its mechanisms of action remain obscure. In our earlier work, we reported that aqueous extract of H. angustifolia root (AQHAR) possesses attractive anticancer properties. In the present study, we isolated five ethanol fractions from AQHAR and investigated their therapeutic efficacy in human non-small cell lung cancer (NSCLC) cells. The results showed that among the five fractions, the 40% ethanol fraction (EF40) containing multiple bioactive compounds exhibited the best selective killing effect on NSCLC cells with no obvious toxicity to normal human fibroblasts...
2023: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/37313306/lncrna-thril-functions-as-a-marker-for-carotid-artery-stenosis-and-affects-the-biological-function-of-human-aortic-endothelial-cell
#8
JOURNAL ARTICLE
Fei Wang, Ying Wang, Xichun Guo
PURPOSE: Carotid artery restenosis (CAS) is a leading contributor to cerebrovascular diseases and one of the leading causes of death in the world. The purpose of this study was to assess the predictive efficiency of long non-coding RNA (lncRNA) TNFalpha-and hnRNP L-related immunoregulatory lncRNA (THRIL) and its association with the pathogenesis of CAS. PATIENTS AND METHODS: The expression of THRIL was determined in patients with asymptomatic CAS and human aortic endothelial cell (HAEC) models induced by oxidized low-density lipoprotein (ox-LDL)...
2023: Journal of Inflammation Research
https://read.qxmd.com/read/37294900/biallelic-variants-in-rbm42-cause-a-multisystem-disorder-with-neurological-facial-cardiac-and-musculoskeletal-involvement
#9
JOURNAL ARTICLE
Yiyao Chen, Bingxin Yang, Xiaoyu Merlin Zhang, Songchang Chen, Minhui Wang, Liya Hu, Nina Pan, Shuyuan Li, Weihui Shi, Zhenhua Yang, Li Wang, Yajing Tan, Jian Wang, Yanlin Wang, Qinghe Xing, Zhonghua Ma, Jinsong Li, He-Feng Huang, Jinglan Zhang, Chenming Xu
Here, we report a previously unrecognized syndromic neurodevelopmental disorder associated with bi-allelic loss-of-function variants in the RBM42 gene. The patient is a two-year-old female with severe central nervous system abnormalities, hypotonia, hearing loss, congenital heart defects, and dysmorphic facial features. Familial whole-exome sequencing reveals that the patient has two compound heterozygous variants, c.304C>T (p.R102*) and c.1312G>A (p.A438T), in the RBM42 gene which encodes an integral component of splicing complex in the RNA-binding motif protein family...
June 9, 2023: Protein & Cell
https://read.qxmd.com/read/37131644/decoding-complexity-in-biomolecular-recognition-of-dna-i-motifs
#10
Kamyar Yazdani, Srinath Seshadri, Desiree Tillo, Charles Vinson, John S Schneekloth
DNA i-motifs (iMs) are non-canonical C-rich secondary structures implicated in numerous cellular processes. Though iMs exist throughout the genome, our understanding of iM recognition by proteins or small molecules is limited to a few examples. We designed a DNA microarray containing 10,976 genomic iM sequences to examine the binding profiles of four iM-binding proteins, mitoxantrone, and the iMab antibody. iMab microarray screens demonstrated that pH 6.5, 5% BSA buffer was optimal, and fluorescence was correlated with iM C-tract length...
April 21, 2023: bioRxiv
https://read.qxmd.com/read/36933825/unravelling-the-tripartite-interactions-among-hepatitis-e-virus-rna-mir-140-and-hnrnp-k
#11
JOURNAL ARTICLE
Rajashree Patil, Pooja Salunke, Yogesh A Karpe
In the present investigation, we have identified the functional significance of the highly conserved miR-140 binding site on the Hepatitis E Virus (HEV) genome. Multiple sequence alignment of the viral genome sequences along with RNA folding prediction indicated that the putative miR-140 binding site has significant conservation for sequence and secondary RNA structure among HEV genotypes. Site-directed mutagenesis and reporter assays indicated that an intact sequence of the miR-140 binding site is essential for HEV translation...
March 17, 2023: Journal of Molecular Biology
https://read.qxmd.com/read/36842269/hnrnp-k-reduces-viral-gene-expression-by-targeting-cytosine-rich-sequences-in-porcine-reproductive-and-respiratory-syndrome-virus-2-genome-to-dampen-the-viral-growth
#12
JOURNAL ARTICLE
Huiyuan Jing, Yuzhen Song, Huawei Li, Erzhen Duan, Jie Liu, Wenting Ke, Ran Tao, Yang Li, Pandeng Zhao, Jinhe Wang, Sufang Cao, Haihua Wang, Yanting Sun, Yan Zhang
HnRNP K is a well-known member of HnRNP family proteins that has been implicated in the regulation of protein expression. Currently, the impact of HnRNP K on the reproduction cycle of a broad range of virus were reported, while the precise function for PRRSV was lacking. In this study, we determined that both PRRSV infection and ectopic expression of N protein induced an enrichment of HnRNP K in the cytoplasm. Using RNA pulldown and RNA immunoprecipitation, we described the interactions between the KH2 domain of HnRNP K and cytosine-rich sequences (CRS) in PRRSV genomic RNA corresponding to Nsp7α coding region...
February 22, 2023: Virology
https://read.qxmd.com/read/36783826/a-g-quadruplex-structure-within-the-3-untranslated-region-of-hnrnp-k-affects-its-translation-by-controlling-the-accessibility-of-mir-1249-3p-to-its-binding-site
#13
JOURNAL ARTICLE
Carlan Gray, Bryan Kelleher, Lydia Grimaldi, Mihaela-Rita Mihailescu
No abstract text is available yet for this article.
February 10, 2023: Biophysical Journal
https://read.qxmd.com/read/36736887/epigenetic-control-of-cancer-cell-proliferation-and-cell-cycle-progression-by-hnrnpk-via-promoting-exon-4-inclusion-of-histone-code-reader-spin1
#14
JOURNAL ARTICLE
Di Li, Jihua Guo, Rong Jia
Heterogeneous nuclear ribonucleoprotein K (HNRNPK, hnRNP K), a multifunctional RNA/DNA binding protein, mainly regulates transcription, translation and RNA splicing, and then plays oncogenic roles in many cancers. However, the related mechanisms remain largely unknown. Here, we found that HNRNPK can partially epigenetically regulate cancer cell proliferation via increasing transcription and exon 4-inclusion of SPIN1, an important oncogenic histone code reader. This exon 4 skipping event of SPIN1 generates a long non-coding RNA, followed by the downregulation of SPIN1 protein...
February 1, 2023: Journal of Molecular Biology
https://read.qxmd.com/read/36700980/hnrnp-k-induces-hpv16-oncogene-expression-and-promotes-cervical-cancerization
#15
JOURNAL ARTICLE
Yuanjing Lyu, Li Song, Rui Mao, Chunliang Liu, Meijuan Feng, Caihong Wu, Ruixin Pei, Ling Ding, Jintao Wang
PURPOSE: This study aims to explore the expression of hnRNP K in cervical carcinogenesis and to investigate the regulatory role of hnRNP K on HPV16 oncogene expression as well as biological changes in cervical cancer cells. METHODS: In total 1042 subjects, including 573 with the normal cervix and 469 with different grades of cervical lesions were enrolled in this study to explore the association between hnRNP K and HPV16 oncogene expression in cervical carcinogenesis...
January 26, 2023: Journal of Cancer Research and Clinical Oncology
https://read.qxmd.com/read/36693612/atsiek-an-exd1-like-protein-with-kh-domain-involves-in-salt-stress-response-by-interacting-with-fry2-cpl1
#16
JOURNAL ARTICLE
Xiangxiang Zhang, Qinyu Xie, Lijun Xiang, Zhonghua Lei, Qixiu Huang, Juncheng Zhang, Maohong Cai, Tao Chen
Abiotic stress has great impacts on plant germination, growth and development and crop yield. Therefore, it is important to understand the molecular mechanism of plants response to abiotic stress. In this study, we identified a plant specific protein AtSIEK (stress-induced protein with EXD1-like domain and KH domain) response to salt stress. AtSIEK encodes a hnRNP K homology (KH) protein localized in nucleus. Amino acid sequences analysis found that SIEK protein is specific in plants, containing two domains with EXD1-like domain and KH domain, while SIEK homolog in animals only had EXD1-like domain without KH domain...
January 21, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/36518526/heterogeneous-nuclear-ribonucleoprotein-k-is-overexpressed-in-acute-myeloid-leukemia-and-causes-myeloproliferation-in-mice-via-altered-runx1-splicing
#17
JOURNAL ARTICLE
Marisa J L Aitken, Prerna Malaney, Xiaorui Zhang, Shelley M Herbrich, Lauren Chan, Oscar Benitez, Ashley G Rodriguez, Huaxian Ma, Rodrigo Jacamo, Ruizhi Duan, Todd M Link, Steven M Kornblau, Rashmi Kanagal-Shamanna, Carlos E Bueso-Ramos, Sean M Post
Acute myeloid leukemia (AML) is driven by numerous molecular events that contribute to disease progression. Herein, we identify hnRNP K overexpression as a recurrent abnormality in AML that negatively correlates with patient survival. Overexpression of hnRNP K in murine fetal liver cells results in altered self-renewal and differentiation potential. Further, murine transplantation models reveal that hnRNP K overexpression results in myeloproliferation in vivo . Mechanistic studies expose a direct functional relationship between hnRNP K and RUNX1 -a master transcriptional regulator of hematopoiesis often dysregulated in leukemia...
December 2022: NAR cancer
https://read.qxmd.com/read/36515841/cytological-approaches-to-visualize-intracellular-dynamics-of-rna-binding-proteins-at-active-genes-in-drosophila
#18
JOURNAL ARTICLE
Anand K Singh, Subhash C Lakhotia
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a family of RNA-binding proteins that modulate multiple aspects of gene activity and RNA processing, including transcription, splicing, localization, translation, and decay of RNA. Interaction of hnRNPs with RNA is a highly dynamic but regulated process. Poly(ADP-ribose) polymerase (PARP)-dependent PARylation of different hnRNPs is a well-known posttranslational modification that affects their interactions with RNA. Here, we described a protocol for in situ localization of RNA-binding proteins (RBPs) on giant polytene chromosomes in Drosophila larval salivary glands, which have been widely used to visualize the dynamic binding profiles of various RBPs and other transcription-related proteins at specific loci on chromosomes...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/36452487/poly-rc-binding-proteins-as-pleiotropic-regulators-in-hematopoiesis-and-hematological-malignancy
#19
REVIEW
Huijuan Zhao, Ziqing Wei, Guomin Shen, Yixiang Chen, Xueqin Hao, Sanqiang Li, Rong Wang
Poly(rC)-binding proteins (PCBPs), a defined subfamily of RNA binding proteins, are characterized by their high affinity and sequence-specific interaction with poly-cytosine (poly-C). The PCBP family comprises five members, including hnRNP K and PCBP1-4. These proteins share a relatively similar structure motif, with triple hnRNP K homology (KH) domains responsible for recognizing and combining C-rich regions of mRNA and single- and double-stranded DNA. Numerous studies have indicated that PCBPs play a prominent role in hematopoietic cell growth, differentiation, and tumorigenesis at multiple levels of regulation...
2022: Frontiers in Oncology
https://read.qxmd.com/read/36334888/assessing-the-role-of-intrinsic-disorder-in-rna-binding-protein-function-hnrnp-k-as-a-case-study
#20
JOURNAL ARTICLE
Prerna Malaney, Oscar Benitez, Xiaorui Zhang, Sean M Post
RNA-binding proteins (RBPs) typically bind to RNA in a sequence-specific manner, resulting in post-transcriptional gene regulation. While the various classes of RNA-binding domains are largely structured, flexible linkers are frequently observed between them. Emerging evidence suggests that these unstructured regions may help spatially position the RNA-binding domains allowing for RNA binding and/or may contribute directly to RNA association via certain sequence motifs contained within them. The importance of these unstructured regions is widely appreciated; however, understanding their contribution to RNA binding, protein stability, and function has been difficult to ascertain...
November 2, 2022: Methods: a Companion to Methods in Enzymology
keyword
keyword
42676
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.