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Fei Chen, Andrew D Chisholm, Yishi Jin
Differential mRNA polyadenylation plays an important role in shaping the neuronal transcriptome. In C. elegans , several ankyrin isoforms are produced from the unc-44 locus through alternative polyadenylation. Here, we identify a key role for an intronic polyadenylation site (PAS) in temporal- and tissue-specific regulation of UNC-44/ankyrin isoforms. Removing an intronic PAS results in ectopic expression of the neuronal ankyrin isoform in non-neural tissues. This mis-expression underlies epidermal developmental defects in mutants of the conserved tumor suppressor death-associated protein kinase dapk-1 We have previously reported that the use of this intronic PAS depends on the nuclear polyadenylation factor SYDN-1, which inhibits the RNA polymerase II CTD phosphatase SSUP-72...
February 15, 2017: Development
Lingyun Xiong, Leila Harhaus, Christian Heffinger, Berthold Bickert, Thomas Kremer, Ulrich Kneser, Christoph Hirche
BACKGROUND AND AIM: Scaphoid nonunion (SN) is a challenging state after scaphoid fracture. The posterior interosseous nerve neurectomy (PINN) is often performed adjunctively with scaphoid reconstruction using autologous bone grafts. However, it remains unclear whether PINN has a prophylactic or therapeutic value, and thus it results superior to patients with SN without PINN. METHODS: A retrospective study was performed to evaluate patients with SN who were treated with autologous bone grafts with (cohort 1) or without PINN (cohort 2) between 1996 and 2010...
August 2015: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Erdem Eren, Gülşah Zeybek, Cenk Ecevit, Seçil Arslanoğlu, Ipek Ergur, Amaç Kiray
INTRODUCTION: Posterior nasal neurectomy is an effective way of treating recalcitrant rhinitis. The aim of this study is to describe the anatomic relationship between the posterior inferior nasal nerve (PINN) and the structures that might be important for posterior nasal neurectomy. MATERIALS AND METHODS: An anatomic study was conducted in a university hospital dissection laboratory with 15 formalin-fixed, sagittally cut adult cadaver heads. The distance between PINN and (1) nasal sill, (2) maxillary sinus ostium, (3) posterior fontanel, (4) torus tubarius, and (5) crista ethmoidalis was measured and the location of PINN with respect to the sphenopalatine artery was assessed to define the exact location of PINN...
May 2015: Journal of Craniofacial Surgery
Hee-Soo Kyung, Seung-Gil Baek, Byoung-Joo Lee, Chang-Hwa Lee
PURPOSE: This study evaluated mid-term results of anterior cruciate ligament (ACL) reconstruction using the PINN-ACL CrossPin system that allowed for short graft fixation. MATERIALS AND METHODS: Forty-three patients underwent single-bundle ACL reconstruction with a 4-strand semitendinosus tendon graft using the PINN-ACL CrossPin system. Femoral fixation was done using the PINN-ACL CrossPin system, and the tibial side was fixed with post-tie and a bioabsorbable interference screw...
March 2015: Knee Surgery & related Research
LaPrincess C Brewer, Sharonne N Hayes, Monica W Parker, Joyce E Balls-Berry, Michele Y Halyard, Vivian W Pinn, Carmen Radecki Breitkopf
OBJECTIVE: To examine perceptions and attitudes toward health-related research participation among professional African American women. METHODS: Participants were members of an African American women's service organization, The Links, Incorporated. Data were collected via self-administered questionnaires at The Links, Incorporated 2012 National Assembly. Sociodemographics, prior research experience, intention to participate (ITP), willingness to participate (WTP) in a variety of research studies and attitudes about research participation were measured...
August 2014: Journal of Women's Health
Ciaran Skerry, Michael L Pinn, Natalie Bruiners, Richard Pine, Maria L Gennaro, Petros C Karakousis
BACKGROUND: The need to develop new, improved treatments for tuberculosis (TB) remains urgent, and the repurposing of existing drugs represents a possible shortcut to market. Recently, there has been significant interest in host-directed adjuvant therapy to enhance bacillary killing. HMG-CoA reductase inhibitors (statins), which are among the most commonly prescribed drugs, have immunomodulatory properties and improve the clinical outcomes of bacterial infections. METHODS: We studied the tuberculocidal activity of simvastatin alone and in combination with first-line anti-TB drugs in J774 macrophages and during chronic TB infection...
September 2014: Journal of Antimicrobial Chemotherapy
Vivian W Pinn
It is remarkable that only a few years ago, discussions about women's health research often had to be prefaced with the expanded vision of what that concept should actually be in totality as the traditional approach to the health of women had focused on the reproductive system or "bikini medicine," as Dr Marianne Legato, an internationally recognized specialist in women's health, and others have characterized it. Today, there is an almost universal appreciation of the broader perception of women's health and that research on women includes not just clinical trials but also basic investigation from the molecular level of genes to the many aspects of behavioral and societal influences...
September 2013: Global Advances in Health and Medicine: Improving Healthcare Outcomes Worldwide
Noton K Dutta, Abdullah Alsultan, Thomas J Gniadek, Deborah A Belchis, Michael L Pinn, Khisimuzi E Mdluli, Eric L Nuermberger, Charles A Peloquin, Petros C Karakousis
Strategies involving new drug combinations, as well as new uses of existing drugs, are urgently needed to reduce the time required to cure patients with drug-sensitive or multidrug-resistant (MDR) tuberculosis (TB). We compared the sterilizing activity of the standard first-line antitubercular regimen, rifampin-isoniazid-pyrazinamide (RHZ), with that of the novel regimen PA-824-moxifloxacin-pyrazinamide (PaMZ), which is currently being studied in clinical trials (NCT01498419), in the guinea pig model of chronic TB infection, in which animals develop necrotic granulomas histologically resembling their human counterparts...
August 2013: Antimicrobial Agents and Chemotherapy
Paul D Jepson, Robert Deaville, Karina Acevedo-Whitehouse, James Barnett, Andrew Brownlow, Robert L Brownell, Frances C Clare, Nick Davison, Robin J Law, Jan Loveridge, Shaheed K Macgregor, Steven Morris, Sinéad Murphy, Rod Penrose, Matthew W Perkins, Eunice Pinn, Henrike Seibel, Ursula Siebert, Eva Sierra, Victor Simpson, Mark L Tasker, Nick Tregenza, Andrew A Cunningham, Antonio Fernández
On 9 June 2008, the UK's largest mass stranding event (MSE) of short-beaked common dolphins (Delphinus delphis) occurred in Falmouth Bay, Cornwall. At least 26 dolphins died, and a similar number was refloated/herded back to sea. On necropsy, all dolphins were in good nutritive status with empty stomachs and no evidence of known infectious disease or acute physical injury. Auditory tissues were grossly normal (26/26) but had microscopic haemorrhages (5/5) and mild otitis media (1/5) in the freshest cases. Five lactating adult dolphins, one immature male, and one immature female tested were free of harmful algal toxins and had low chemical pollutant levels...
2013: PloS One
Noton K Dutta, Michael L Pinn, Ming Zhao, Michelle A Rudek, Petros C Karakousis
OBJECTIVES: The antipsychotic drug thioridazine is active in the murine model of tuberculosis infection, which is predominantly intracellular in nature. Recent clinical reports suggest that thioridazine may play a role in the treatment of drug-resistant tuberculosis. We studied the tuberculocidal activity of thioridazine in guinea pigs, which develop necrotic lung granulomas histologically resembling their human counterparts. METHODS: Pharmacokinetic studies were performed in guinea pigs to establish human-equivalent doses of thioridazine...
June 2013: Journal of Antimicrobial Chemotherapy
Toby L Pinn, Lucille F Gagliardo, Steve R Purdy, Judith A Appleton, Tracy Stokol
Measurement of serum immunoglobulin G (IgG) is used for the assessment of passive transfer of immunity in neonatal crias, with an IgG concentration <10 g/l being suggestive of failure of passive transfer (FPT). The purpose of the current study was to determine whether 3 commercially available immunologic assays yielded comparable results for IgG in alpacas. Serum samples from 91 alpacas were used and were stored frozen until batch analysis on the same day with the 3 assays. Immunoglobulin G was measured by radial immunodiffusion (RID) and 2 immunoturbidimetric (IT) assays (IT1, configured for automated chemistry analyzers; IT2, a point-of-care test)...
January 2013: Journal of Veterinary Diagnostic Investigation
Toby L Pinn, Hannah S Bender, Tracy Stokol, Hollis N Erb, Donald H Schlafer, Gillian A Perkins
Aberrant migration of Parelaphostrongylus tenuis in camelids results in neurologic deficits, recumbency, and sometimes death. An antemortem diagnosis of P. tenuis in camelids is typically based upon the presence of characteristic asymmetric neurologic deficits, known exposure to white-tailed deer, cerebrospinal fluid (CSF) eosinophilia, and response to treatment. The diagnostic accuracy of CSF eosinophil percentage for the diagnosis of P. tenuis in camelids has not been critically examined. The objective of the current study was to determine the sensitivity (Se) and specificity (Sp) of CSF eosinophil percentage, CSF eosinophil concentration, total nucleated cell concentration, and protein concentration for the antemortem diagnosis of P...
January 2013: Journal of Veterinary Diagnostic Investigation
Seung-Suk Seo, Chang-Wan Kim, Tae-Seok Nam, Sang-Yeong Choi
PURPOSE: To compare the short term clinical results of anterior cruciate ligament (ACL) reconstruction with autologous hamstring tendon between Rigid-fix and PINN-ACL Cross Pin for femoral side fixation. MATERIALS AND METHODS: 127 patients who underwent arthroscopic ACL reconstruction using autologous hamstring tendon and had been followedup for over than one year were enrolled for the present study. Rigid-fix was used in 71 cases (group 1), and PINN-ACL Cross Pin was used in 56 cases (group 2)...
December 2011: Knee Surgery & related Research
Noton K Dutta, Peter B Illei, Charles A Peloquin, Michael L Pinn, Khisimuzi E Mdluli, Eric L Nuermberger, Jacques H Grosset, Petros C Karakousis
Rifamycins are key sterilizing drugs in the current treatment of active tuberculosis (TB). Daily dosing of rifapentine (P), a potent rifamycin with high intracellular accumulation, in place of rifampin (R) in the standard antitubercular regimen significantly shortens the duration of treatment needed to prevent relapse in a murine model of active TB. We undertook the current study to compare directly the activities of human-equivalent doses of P and R in a guinea pig model of chronic TB, in which bacilli are predominantly extracellular within human-like necrotic granulomas...
July 2012: Antimicrobial Agents and Chemotherapy
Pinn-Tsong Chiang, Johannes Mielke, Jazmin Godoy, Jason M Guerrero, Lawrence B Alemany, Carlos J Villagómez, Alex Saywell, Leonhard Grill, James M Tour
A second generation motorized nanocar was designed, synthesized, and imaged. To verify structural integrity, NMR-based COSY, NOESY, DEPT, HSQC, and HMBC experiments were conducted on the intermediate motor. All signals in (1)H NMR were unambiguously assigned, and the results were consistent with the helical structure of the motor. The nanocar was deposited on a Cu(111) surface, and single intact molecules were imaged by scanning tunneling microscopy (STM) at 5.7 K, thereby paving the way for future single-molecule studies of this motorized nanocar atop planar substrates...
January 24, 2012: ACS Nano
Marija Pinne, David Haake
Bacterial surface proteins are involved in direct contact with host cells and in uptake of nutrients from the environment (1). For this reason, cellular localization can provide insights into the functional role of bacterial proteins. Surface localization of bacterial proteins is a key step towards identification of virulence factors involved in mechanisms of pathogenicity. Methods for fractionating leptospiral membranes (2-5) may be selective for a certain class of outer-membrane proteins (OMPs), such as lipoproteins vs...
2011: Journal of Visualized Experiments: JoVE
C Krischek, R Natter, R Wigger, M Wicke
Longissimus muscle samples from the pig genotypes Duroc (Du), Pietrain (MHS homozygote negative (PiNN), positive (PiPP)) and a Duroc-Pietrain crossbreed (DuPi) were analyzed. The PiPP samples showed a faster pH drop and higher electrical conductivity, drip loss and lightness values. Before slaughter the concentrations of the adenine nucleotides were comparable between the genotypes, but 40 min after slaughter (p.m.) the ATP concentrations decreased and IMP increased, to a higher extent in the PiPP pigs. The nucleotide values of the 12 h p...
October 2011: Meat Science
Zahoor Ahmad, Michael L Pinn, Eric L Nuermberger, Charles A Peloquin, Jacques H Grosset, Petros C Karakousis
OBJECTIVES: The biphasic kill curve of isoniazid against Mycobacterium tuberculosis in guinea pigs is due to the presence of persisters rather than selection of isoniazid-resistant mutants. To determine whether this phenomenon is common to other bactericidal drugs, we studied the activity of streptomycin and its ability to select for streptomycin-resistant mutants in the guinea pig model of tuberculosis. METHODS: Pharmacokinetic studies were performed to establish the human-equivalent dose of streptomycin...
October 2010: Journal of Antimicrobial Chemotherapy
Daniel M Rackham, Shaunta' M Ray, Andrea S Franks, Kenneth M Bielak, Tanika M Pinn
OBJECTIVE: This study evaluated the prevalence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) nasal carriage, risk factors for nasal carriage, and antibiotic susceptibility patterns in college student athletes. DESIGN: Cross-sectional study. SETTING: Methicillin-resistant Staphylococcus aureus nasal samples and data collection forms were obtained at athletic team training rooms at 2 colleges. PARTICIPANTS: : The study population included 277 college student athletes older than 18 years...
May 2010: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
Stacy Wentworth, Melva Pinn, J Daniel Bourland, Allan F Deguzman, Kenneth Ekstrand, Thomas L Ellis, Steven S Glazier, Kevin P McMullen, Michael Munley, Volker W Stieber, Stephen B Tatter, Edward G Shaw
PURPOSE: Patients with neurofibromatosis (NF) develop tumors of the central nervous system (CNS). Radiation therapy (RT) is used to treat these lesions. To better define the efficacy of RT in these patients, we reviewed our 20-year experience. METHODS AND MATERIALS: Eighteen patients with NF with CNS tumors were treated from 1986 to 2007. Median follow-up was 48 months. Progression was defined as growth or recurrence of an irradiated tumor on serial imaging. Progression-free survival (PFS) was measured from the date of RT completion to the date of last follow-up imaging study...
January 1, 2009: International Journal of Radiation Oncology, Biology, Physics
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