keyword
MENU ▼
Read by QxMD icon Read
search

pin1

keyword
https://www.readbyqxmd.com/read/29157862/scutellarin-inhibits-hela-cell-growth-and-glycolysis-by-inhibiting-the-activity-of-pyruvate-kinase-m2
#1
Lin You, Hong Zhu, Chun Wang, Fang Wang, Yongjun Li, Yan Li, Yonglin Wang, Bin He
Scutellarin, one of natural flavonoids, is widely and clinically used for treating many diseases in China. Recently, scutellarin has demonstrated a broad spectrum of anti-proliferative activities against multiple cancer cell lines. However, the molecular mechanism of action remains to be investigated. We herein report the design and synthesis of biotinylated scutellareins as probes, which can be applied to discover scutellarein interacting proteins. Finally, we show that scutellarin directly targets pyruvate kinase M2 (PKM2) and inhibits its cytosolic activity to decrease glycolytic metabolism; on the other hand, scutellarin may also participate in regulating cell cycle and apoptotic proteins by activating MEK/ERK/PIN1 signaling pathway to promote the nuclear translocation of PKM2...
November 10, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29139551/members-of-the-deal-subfamily-of-the-duf1218-gene-family-are-required-for-bilateral-symmetry-but-not-for-dorsoventrality-in-arabidopsis-leaves
#2
David Wilson-Sánchez, Sebastián Martínez-López, Sergio Navarro-Cartagena, Sara Jover-Gil, José Luis Micol
Most plant leaves exhibit bilateral symmetry, which has been hypothesized as an inevitable consequence of the existence of the proximodistal and dorsoventral axes. No gene has been described that affects leaf bilateral symmetry but not dorsoventrality in Arabidopsis thaliana. We screened for viable insertional mutations that affect leaf morphology, and out of more than 700 mutants found only one, desigual1-1 (deal1-1), that exhibited bilateral symmetry breaking but no obvious defects in dorsoventrality. We found that deal1-1 is an allele of VASCULATURE COMPLEXITY AND CONNECTIVITY (VCC)...
November 15, 2017: New Phytologist
https://www.readbyqxmd.com/read/29119309/localization-and-transport-of-indole-3-acetic-acid-during-somatic-embryogenesis-in-coffea-canephora
#3
Ruth E Márquez-López, Cleyre Pérez-Hernández, Ángela Ku-González, Rosa María Galaz-Ávalos, Víctor Manuel Loyola-Vargas
Auxin and polar auxin transport have been implicated in controlling zygotic embryo development, but less is known about their role in the development of somatic embryos. The aim of this study was to determine if indole-3-acetic acid (IAA) and the PIN1 transporter participate in the induction of somatic embryogenesis (SE) and the development of somatic embryos. The results show that IAA levels gradually increase during pre-treatment and accumulate in the chloroplast. During pre-treatment and the globular stage of SE in C...
November 8, 2017: Protoplasma
https://www.readbyqxmd.com/read/29118189/the-peptidyl-prolyl-isomerase-pin1-relieves-cyclin-dependent-kinase-2-cdk2-inhibition-by-the-cdk-inhibitor-p27
#4
Chi-Wai Cheng, Ka-Wai Leong, Yiu-Ming Ng, Yok-Lam Kwong, Eric Tse
PIN1 is a peptidyl-prolyl-isomerase that catalyzes the cis/trans isomerization of peptide bonds between proline and phosphorylated serine/threonine residues. By changing the conformation of its protein substrates, PIN1 increases the activities of key proteins that promote cell cycle progression and oncogenesis. Moreover, it has been shown that PIN1 stabilizes and increases the level of the cyclin-dependent kinase (CDK) inhibitor p27, which inhibits cell cycle progression by binding cyclin A- and cyclin E-CDK2...
November 8, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29113110/two-paralogous-genes-encoding-auxin-efflux-carrier-differentially-expressed-in-bitter-gourd-momordica-charantia
#5
Yi-Li Li, Yun-Shan Lin, Pung-Ling Huang, Yi-Yin Do
The phytohormone auxin regulates various developmental programs in plants, including cell growth, cell division and cell differentiation. The auxin efflux carriers are essential for the auxin transport. To show an involvement of auxin transporters in the coordination of fruit development in bitter gourd, a juicy fruit, we isolated novel cDNAs (referred as McPIN) encoding putative auxin efflux carriers, including McPIN1, McPIN2 (allele of McPIN1) and McPIN3, from developing fruits of bitter gourd. Both McPIN1 and McPIN3 genes possess six exons and five introns...
November 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29078092/the-allelochemical-farnesene-affects-arabidopsis-thaliana-root-meristem-altering-auxin-distribution
#6
Fabrizio Araniti, Leonardo Bruno, Francesco Sunseri, Marianna Pacenza, Ivano Forgione, Maria Beatrice Bitonti, Maria Rosa Abenavoli
Farnesene is a sesquiterpene with semiochemical activity involved in interspecies communication. This molecule, known for its phytotoxic potential and its effects on root morphology and anatomy, caused anisotropic growth, bold roots and a "left-handedness" phenotype. These clues suggested an alteration of auxin distribution, and for this reason, the aim of the present study was to evaluate its effects on: i) PIN-FORMED proteins (PIN) distribution, involved in polar auxin transport; ii) PIN genes expression iii) apical meristem anatomy of primary root, in 7 days old Arabidopsis thaliana seedlings treated with farnesene 250 μM...
October 19, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29068222/stereoselective-deuteration-in-aspartate-asparagine-lysine-and-methionine-amino-acid-residues-using-fumarate-as-a-carbon-source-for-escherichia-coli-in-d2o
#7
Gaddafi I Danmaliki, Philip B Liu, Peter M Hwang
Perdeuteration with selective (1)H,(13)C-enrichment of methyl groups has enabled solution NMR studies of large (>30 kDa) protein systems. However, we propose that for all non-methyl positions, only magnetization originating from (1)H-(12)C groups is sufficiently long-lived, and it can be transferred via through-space NOEs to slowly relaxing (1)H-(15)N or (1)H-(13)C methyl groups to achieve multidimensional solution NMR. We demonstrate stereoselective (1)H,(12)C-labeling by adding relatively inexpensive unlabeled carbon sources to Escherichia coli growth media in D2O...
November 3, 2017: Biochemistry
https://www.readbyqxmd.com/read/29064367/cross-species-functional-diversity-within-the-pin-auxin-efflux-protein-family
#8
Devin Lee O'Connor, Samuel Elton, Fabrizio Ticchiarelli, Mon Mandy Hsia, John P Vogel, Ottoline Leyser
In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated by polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which is conserved in flowering plants. We previously proposed that the AtPIN1 organ initiation and vein patterning functions are split between the SoPIN1 and PIN1 clades in grasses. Here we show that in the grass Brachypodium sopin1 mutants have organ initiation defects similar to Arabidopsis atpin1, while loss of PIN1 function in Brachypodium has little effect on organ initiation but alters stem growth...
October 24, 2017: ELife
https://www.readbyqxmd.com/read/29062054/integrated-analysis-of-mrna-and-viral-mirnas-in-the-kidney-of-carassius-auratus-gibelio-response-to-cyprinid-herpesvirus-2
#9
Jianfei Lu, Dan Xu, Yousheng Jiang, Shanyun Kong, Zhaoyuan Shen, Siyao Xia, Liqun Lu
MicroRNAs (miRNAs) are small, non-coding single stranded RNAs that play crucial roles in numerous biological processes. Vertebrate herpesviruses encode multiple viral miRNAs that modulate host and viral genes. However, the roles of viral miRNAs in lower vertebrates have not been fully determined. Here, we used high-throughput sequencing to analyse the miRNA and mRNA expression profiles of Carassius auratus gibelio in response to infection by cyprinid herpesvirus 2 (CyHV-2). RNA sequencing obtained 26,664 assembled transcripts, including 2,912 differentially expressed genes...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29059380/expression-of-arabidopsis-class-1-phytoglobin-atpgb1-delays-death-and-degradation-of-the-root-apical-meristem-during-severe-peg-induced-water-deficit
#10
Mohamed M Mira, Shuanglong Huang, Karuna Kapoor, Cassandra Hammond, Robert D Hill, Claudio Stasolla, Ian Dodd
Maintenance of a functional root is fundamental to plant survival in response to some abiotic stresses, such as water deficit. In this study, we found that overexpression of Arabidopsis class 1 phytoglobin (AtPgb1) alleviated the growth retardation of polyethylene glycol (PEG)-induced water stress by reducing programmed cell death (PCD) associated with protein folding in the endoplasmic reticulum (ER). This was in contrast to PEG-stressed roots down-regulating AtPgb1 that exhibited extensive PCD and reduced expression of several attenuators of ER stress, including BAX Inhibitor-1 (BI-1)...
October 20, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29050342/role-of-the-cyp3a4-mediated-11-12-epoxyeicosatrienoic-acid-pathway-in-the-development-of-tamoxifen-resistant-breast-cancer
#11
Nguyen Thi Thuy Phuong, Ji Won Kim, Jung-Ae Kim, Jang Su Jeon, Ji-Yoon Lee, Wen Jun Xu, Jin Won Yang, Sang Kyum Kim, Keon Wook Kang
Epoxyeicosatrienoic acid (EET) production via cytochrome P450 (CYP) epoxygenases closely correlates with the progression of breast cancer. However, its role in the development of chemoresistant breast cancers has yet to be elucidated. Here, we found that CYP3A4 expression and its epoxy-product, 11,12-epoxyeicosatrienoic acid (11,12-EET) was enhanced in tamoxifen (TAM)-resistant MCF-7 (TAMR-MCF-7) breast cancer cells compared to control MCF-7 cells. Treatment of TAMR-MCF-7 cells with ketoconazole and azamulin (selective CYP3A4 inhibitors) or 14,15-epoxyeicosa-5(Z)-enoic acid (14,15-EEZE, an EET antagonist) inhibited cellular proliferation and recovered the sensitivity to 4-hydroxytamoxifen...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29040060/structure-and-function-of-the-human-parvulins-pin1-and-par14-17
#12
Anja Matena, Edisa Rehic, Dana Hönig, Bianca Kamba, Peter Bayer
Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell fate decisions such as cell-cycle progression, metabolic pathways and ribosome biogenesis. This review on human parvulins summarizes the current knowledge of these enzymes and intends to oppose the well-studied Pin1 to its less well-examined homolog human Par14/17 with respect to structure, catalytic and cellular function...
October 26, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29031033/an-arabidopsis-divergent-pumilio-protein-apum24-is-essential-for-embryogenesis-and-required-for-faithful-pre-rrna-processing
#13
Thiruvenkadam Shanmugam, Nazia Abbasi, Hyung-Sae Kim, Ho Bang Kim, Nam-Il Park, Guen Tae Park, Sung Aeong Oh, Soon Ki Park, Douglas G Muench, Yeonhee Choi, Youn-Il Park, Sang-Bong Choi
Pumilio RNA-binding proteins are largely involved in mRNA degradation and translation repression. However, a few evolutionarily divergent Pumilios are also responsible for proper pre-rRNA processing in human and yeast. Here, we describe an essential Arabidopsis nucleolar Pumilio, APUM24, that is expressed in tissues undergoing rapid proliferation and cell division. A T-DNA insertion for APUM24 did not affect the male and female gametogenesis, but instead resulted in a negative female gametophytic effect on zygotic cell division immediately after fertilization...
October 14, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29018025/peptidyl-prolyl-isomerase-1-regulates-ca-2-handling-by-modulating-sarco-endo-plasmic-reticulum-calcium-atpase-and-na-2-ca-2-exchanger-1-protein-levels-and-function
#14
Veronica Sacchi, Bingyan J Wang, Dieter Kubli, Alexander S Martinez, Jung-Kang Jin, Roberto Alvarez, Nirmala Hariharan, Christopher Glembotski, Takafumi Uchida, James S Malter, Yijun Yang, Polina Gross, Chen Zhang, Steven Houser, Marcello Rota, Mark A Sussman
BACKGROUND: Aberrant Ca(2+) handling is a prominent feature of heart failure. Elucidation of the molecular mechanisms responsible for aberrant Ca(2+) handling is essential for the development of strategies to blunt pathological changes in calcium dynamics. The peptidyl-prolyl cis-trans isomerase peptidyl-prolyl isomerase 1 (Pin1) is a critical mediator of myocardial hypertrophy development and cardiac progenitor cell cycle. However, the influence of Pin1 on calcium cycling regulation has not been explored...
October 10, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29016944/auxin-response-dynamics-during-wild-type-and-entire-flower-development-in-tomato
#15
Shiri Goldental-Cohen, Alon Israeli, Naomi Ori, Hagai Yasuor
The plant hormone auxin is a major regulator of plant development and response to environmental cues. Auxin plays a particularly central role in flower development, but the knowledge of its role of flower development in crop plants with fleshy fruits, such as tomato, is still scarce. Mutations in the Aux/IAA gene ENTIRE/Indole Acetic Acid 9 (E/IAA9) lead to the precocious development of young gynoecia into parthenocarpic fruits. Here, we compared the distribution of the auxin response sensor DR5::VENUS and the auxin efflux transporter PIN1 between the wild type and entire during successive stages of flower and fruit development...
July 31, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29016942/ben3-big2-arf-gef-is-involved-in-brefeldin-a-sensitive-trafficking-at-the-trans-golgi-network-early-endosome-in-arabidopsis-thaliana
#16
Saeko Kitakura, Maciek Adamowski, Yuki Matsuura, Luca Santuari, Hirotaka Kouno, Kohei Arima, Christian S Hardtke, Jirí Friml, Tatsuo Kakimoto, Hirokazu Tanaka
Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA), which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM...
August 21, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28993788/lanthanum-inhibits-primary-root-growth-by-repressing-auxin-carrier-abundances-in-arabidopsis
#17
Yangyang Liu, Liangliang Sun, Ping Zhang, Jinpeng Wan, Ruling Wang, Jin Xu
Lanthanum (La) is one of rare earth elements that was used as a crop growth stimulants; however, high concentration of La markedly inhibited plant growth. Our previous study indicated that, although La induced the expression of auxin biosynthesis-related genes, it markedly repressed primary root (PR) elongation by reducing auxin accumulation in PR tips. In this study, we exhibited that La reduces the abundances of auxin carriers. Treatment with La markedly inhibited the auxins IAA-, 2,4-D-, and NAA-induced elevation of DR5:GUS activity in the roots, suggesting that La inhibited auxin transport through both the influx and efflux transporters...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28986099/downregulation-of-pin1-in-human-atherosclerosis-and-its-association-with-vascular-smooth-muscle-cell-senescence
#18
Lei Lv, Meng Ye, Rundan Duan, Kai Yuan, Jiaquan Chen, Wei Liang, Zhaoxiong Zhou, Lan Zhang
OBJECTIVE: Pin1 is prevalently overexpressed in human cancers and implicated to regulate cell growth and apoptosis. Thus far, however, no role for Pin1 has been described in modulating vascular smooth muscle cell (VSMC) senescence. METHODS: Immunohistochemistry and Western blotting were used to assess Pin1 protein level in human normal and atherosclerotic tissues. β-galactosidase staining, cumulative population doubling level, telomerase activity, and relative telomere length measurement were used to confirm VSMC senescence...
October 3, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28971096/protein-translation-and-signaling-in-human-eosinophils
#19
REVIEW
Stephane Esnault, Zhong-Jian Shen, James S Malter
We have recently reported that, unlike IL-5 and GM-CSF, IL-3 induces increased translation of a subset of mRNAs. In addition, we have demonstrated that Pin1 controls the activity of mRNA binding proteins, leading to enhanced mRNA stability, GM-CSF protein production and prolonged eosinophil (EOS) survival. In this review, discussion will include an overview of cap-dependent protein translation and its regulation by intracellular signaling pathways. We will address the more general process of mRNA post-transcriptional regulation, especially regarding mRNA binding proteins, which are critical effectors of protein translation...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/28969797/folic-acid-orchestrates-root-development-linking-cell-elongation-with-auxin-response-and-acts-independently-of-the-target-of-rapamycin-signaling-in-arabidopsis-thaliana
#20
Juan Ángel Ayala-Rodríguez, Salvador Barrera-Ortiz, León Francisco Ruiz-Herrera, José López-Bucio
Folic acid is a precursor of tetrahydrofolate (vitamin B9), which is an essential cofactor in most organisms, acting as a carrier for one-carbon units in enzymatic reactions. In this work, we employed pharmacological, genetic and confocal imaging strategies to unravel the signaling mechanism by which folic acid modulates root growth and development. Folic acid supplementation inhibits primary root elongation and induces lateral root formation in a concentration-dependent manner. An analysis of the expression of cell cycle genes pCycD6;1:GFP and CycB1:uidA, and cell expansion Exp7:uidA showed that folic acid promotes cell division but prevented cell elongation, and this correlated with altered expression of auxin-responsive DR5:GFP gene, and PIN1:PIN1:GFP, PIN3:PIN3:GFP, and PIN7:PIN7:GFP auxin transporters at the columella and vasculature of primary roots, whereas mutants defective in auxin signaling (tir1/afb1/afb2 [receptors], slr1 [repressor] and arf7/arf19 [transcription factors]) were less sensitive to folic acid induced primary root shortening and lateral root proliferation...
November 2017: Plant Science: An International Journal of Experimental Plant Biology
keyword
keyword
42673
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"