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https://www.readbyqxmd.com/read/28323446/leguminosae-native-nodulating-bacteria-from-a-gold-mine-as-contaminated-soil-multi-resistance-to-trace-elements-and-possible-role-in-plant-growth-and-mineral-nutrition
#1
Wesley de M Rangel, Silvia M de Oliveira Longatti, Paulo A A Ferreira, Daiane S Bonaldi, Amanda A Guimarães, Sofie Thijs, Nele Weyens, Jaco Vangronsveld, Fatima M S Moreira
Efficient N2-fixing Leguminosae nodulating bacteria resistant to As may facilitate plant growth on As-contaminated sites. In order to identify bacteria possessing these features, 24 strains were isolated from nodules of the trap species Crotalaria spectabilis (12) and Stizolobium aterrimum (12) growing on an As-contaminated gold mine site. 16S rRNA gene sequencing revealed that most of the strains belonged to the group of α-Proteobacteria, being representatives of the genera Bradyrhizobium, Rhizobium, Inquilinus, Labrys, Bosea, Starkeya and Methylobacterium...
March 21, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28319382/time-resolved-spectroscopic-investigation-of-charge-trapping-in-carbon-nitrides-photocatalysts-for-hydrogen-generation
#2
Robert Godin, Yiou Wang, Martijn A Zwijnenburg, Junwang Tang, James R Durrant
Carbon nitride (g-C3N4) as a benchmark polymer photocatalyst is attracting significant research interest because of its visible light photocatalytic performance combined with good stability and facile synthesis. However, little is known about the fundamental photophysical processes of g-C3N4, which are key to explain and promote photoactivity. Using time-resolved absorption and photoluminescence spectroscopies, we have investigated the photophysics of a series of carbon nitrides on timescales ranging from femtoseconds to seconds...
March 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28318591/reduction-of-mycobacterium-avium-ssp-paratuberculosis-in-colostrum-development-and-validation-of-2-methods-one-based-on-curdling-and-one-based-on-centrifugation
#3
M Verhegghe, G Rasschaert, L Herman, K Goossens, L Vandaele, K De Bleecker, G Vlaemynck, M Heyndrickx, J De Block
The aim of this study was to develop and validate 2 protocols (for use on-farm and at a central location) for the reduction of Mycobacterium avium ssp. paratuberculosis (MAP) in colostrum while preserving beneficial immunoglobulins (IgG). The on-farm protocol was based on curdling of the colostrum, where the IgG remain in the whey and the MAP bacteria are trapped in the curd. First, the colostrum was diluted with water (2 volumes colostrum to 1 volume water) and 2% rennet was added. After incubation (1 h at 32°C), the curd was cut and incubated again, after which whey and curd were separated using a cheesecloth...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318239/trapping-experiments-on-a-trichlorosilanide-anion-a-key-intermediate-of-halogenosilane-chemistry
#4
Julian Teichmann, Markus Bursch, Benedikt Köstler, Michael Bolte, Hans-Wolfram Lerner, Stefan Grimme, Matthias Wagner
Treatment of Si2Cl6 with [Et4N][BCl4] in CH2Cl2 furnished the known products of a chloride-induced disproportionation reaction of the disilane, such as SiCl4, [Si(SiCl3)3](-), and [Si6Cl12·2Cl](2-). No Si-B-bonded products were detectable. In contrast, the addition of Si2Cl6 to [Et4N][BI3Cl] afforded the Si-B adduct [Et4N][I3SiBI3]. Thus, a quantitative Cl/I exchange at the silicon atom accompanies the trihalogenosilanide formation. [Et4N][I3SiBI3] was also accessible from a mixture of Si2I6, [Et4N]I, and BI3...
March 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28317941/magnitude-dependent-response-of-osteoblasts-regulated-by-compressive-stress
#5
Xiao-Qing Shen, Yuan-Ming Geng, Ping Liu, Xiang-Yu Huang, Shu-Yi Li, Chun-Dong Liu, Zheng Zhou, Ping-Ping Xu
The present study aimed to investigate the role of magnitude in adaptive response of osteoblasts exposed to compressive stress. Murine primary osteoblasts and MC3T3-E1 cells were exposed to compressive stress (0, 1, 2, 3, 4, and 5 g/cm(2)) in 3D culture. Cell viability was evaluated, and expression levels of Runx2, Alp, Ocn, Rankl, and Opg were examined. ALP activity in osteoblasts and TRAP activity in RAW264.7 cells co-cultured with MC3T3-E1 cells were assayed. Results showed that compressive stress within 5...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28317264/a-collection-of-enhancer-trap-insertional-mutants-for-functional-genomics-in-tomato
#6
Fernando Pérez-Martín, Fernando J Yuste-Lisbona, Benito Pineda, María Pilar Angarita-Díaz, Begoña García-Sogo, Teresa Antón, Sibilla Sánchez, Estela Giménez, Alejandro Atarés, Antonia Fernández-Lozano, Ana Ortíz-Atienza, Manuel García-Alcázar, Laura Castañeda, Rocío Fonseca, Carmen Capel, Geraldine Goergen, Jorge Sánchez, Jorge L Quispe, Juan Capel, Trinidad Angosto, Vicente Moreno, Rafael Lozano
With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of phenotypic variations between a wild-type and its mutant; hence, mutant collections are a valuable resource. In this sense, T-DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T-DNA collection in tomato (Solanum lycopersicum L...
March 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28315458/cyperus-rotundus-l-extract-suppresses-rankl-induced-osteoclastogenesis-through-nfatc1-c-fos-downregulation-and-prevent-bone-loss-in-ovx-induced-osteoporosis-rat
#7
Chang-Jun Kum, Eun-Young Kim, Jae-Hyun Kim, Bina Lee, Ju-Hee Min, Jun Heo, Jong-Hyun Kim, Mijung Yeom, Youngjoo Sohn, Hyuk-Sang Jung
ETHNOPHARMACOLOGICAL RELEVANCE: Cyperus Rotundus L. (CyR) has been widely used for the treatment of gynecologic disorder. Recent studies have reported that CyR can prevent the formation of cystic follicles and ovarian malfunction. However, the effects of CyR on osteoclastogenesis and postmenopausal osteoporosis remain unknown. AIM OF THE STUDY: This study was aimed to investigate the preventive effects of CyR on RANKL-induced osteoclast formation and ovariectomy (OVX)-induced bone loss...
March 15, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28314990/an-apparent-clinical-pharmacokinetic-drug-drug-interaction-between-bevacizumab-and-the-anti-placental-growth-factor-monoclonal-antibody-ro5323441-via-a-target-trapping-mechanism
#8
Ka Wang, Franziska Schaedeli Stark, Tilman Schlothauer, Angelika Lahr, Valerie Cosson, Jianguo Zhi, Kai Habben, Jean Tessier, Eginhard Schick, Roland F Staack, Oliver Krieter
PURPOSE: RO5323441 is a humanized anti-placental growth factor (PlGF) monoclonal antibody that has shown preclinical activity in several cancer models. The objective of this analysis is to examine the pharmacokinetic (PK) results from four Phase I studies that have been conducted with RO5323441 (n = 61) and to report an apparent drug-drug interaction observed when RO5323441 was administered in combination with bevacizumab. METHODS: The four Phase I studies were a multiple-ascending dose study in 23 patients with solid tumors (Study 1), an open-label study in seven patients with colorectal/ovarian cancer (Study 2), a sorafenib combination study in nine patients with hepatocellular carcinoma (Study 3), and a bevacizumab combination study in 22 patients with recurrent glioblastoma (Study 4)...
March 17, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28314431/fast-analysis-of-phthalates-in-freeze-dried-baby-foods-by-ultrasound-vortex-assisted-liquid-liquid-microextraction-coupled-with-gas-chromatography-ion-trap-mass-spectrometry
#9
Mario Vincenzo Russo, Pasquale Avino, Ivan Notardonato
This paper is focused on the determination of phthalates (PAEs), compounds "plausibly" endocrine disruptors, in baby food products by means of a method based on ultrasound-vortex-assisted liquid-liquid microextraction coupled with GC-IT/MS (UVALLME-GC-IT/MS). Particularly, the whole procedure allows the determination of six phthalates such as DMP, DEP, DBP, iBcEP, BBP and DEHP. After dissolution of 0.1g product sample and addition of anthracene as Internal Standard, 250μL of n-heptane are used as extraction solvent...
November 25, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28306318/magnetic-trapping-of-cold-methyl-radicals
#10
Yang Liu, Manish Vashishta, Pavle Djuricanin, Sida Zhou, Wei Zhong, Tony Mittertreiner, David Carty, Takamasa Momose
We have demonstrated that a supersonic beam of methyl radicals (CH_{3}) in the ground rotational state of both para and ortho species has been slowed down to standstill with a magnetic molecular decelerator, and successfully captured spatially in an anti-Helmholtz magnetic trap for >1  s. The trapped CH_{3} radicals have a mean translational temperature of about 200 mK with an estimated density of >5.0×10^{7}  cm^{-3}. The methyl radical is an ideal system for the study of cold molecules not only because of its high reactivities at low temperatures, but also because further cooling below 1 mK is plausible via sympathetic cooling with ultracold atoms...
March 3, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28306254/white-light-emission-and-structural-distortion-in-new-corrugated-2d-lead-bromide-perovskites
#11
Lingling Mao, Yilei Wu, Constantinos C Stoumpos, Michael R Wasielewski, Mercouri G Kanatzidis
Hybrid inorganic-organic perovskites are developing rapidly as high performance semiconductors. Recently, two-dimensional (2D) perovskites were found to have white-light, broadband emission in the visible range and was attributed mainly to the role of self-trapped excitons (STEs). Here, we describe three new 2D lead bromide perovskites incorporating a series of bifunctional ammonium di-cations as templates which also emit white light: 1) α-(DMEN)PbBr4 (DMEN = 2-(Dimethylamino)ethylamine), which adopts a unique corrugated layered structure in space group Pbca with unit cell a =18...
March 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28306240/atomic-layer-etching-mechanism-of-mos2-for-nano-devices
#12
Ki Seok Kim, Ki Hyun Kim, Yeonsig Nam, Jaeho Jeon, Soonmin Yim, Eric Singh, Jin Yong Lee, Sungjoo Lee, Yeon Sik Jung, Geun Young Yeom, Dong Woo Kim
Among the layered transition metal dichalcogenides (TMDs), which can form stable two-dimensional crystal structures, molybdenum disulfide (MoS2) has been intensively investigated in various electronic and optoelectronic applications, according to different band-gap energies from 1.29 eV to 1.9 eV as the number of layers is decreased, due to its unique properties. To control the MoS2 layers, atomic layer etching (ALE), a cyclic etching consisting of a radical-adsorption step such as Cl adsorption and a reacted-compound desorption step that is through a low-energy Ar(+)-ion exposure, can be a highly effective technique that avoids the inducement of damage and the contamination that are from the reactive steps...
March 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28304122/comparative-study-of-magnetic-resonance-defaecography-and-evacuation-proctography-in-the-evaluation-of-obstructed-defaecation
#13
A Zafa, C Seretis, M Feretis, S Karandikar, S C Williams, M Goldstein, M Chapman
AIM: Obstructed defaecation syndrome (ODS) is a common condition of multifactorial aetiology and requires specialised evaluation. Accurate and reproducible pelvic floor imaging is imperative for multidisciplinary decision-making. Evacuation Proctography (EP) and Magnetic Resonance Defaecography (MRD) are the main imaging modalities used to assess dynamic pelvic floor function. The aim of this prospective study was to compare the findings and acceptability of MRD and EP in the same cohort of patients...
March 17, 2017: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/28303858/role-of-neutrophil-extracellular-traps-in-asthma-and-chronic-obstructive-pulmonary-disease
#14
REVIEW
Ting Liu, Fa-Ping Wang, Geng Wang, Hui Mao
OBJECTIVE: Asthma and chronic obstructive pulmonary disease (COPD) are representative chronic inflammatory airway diseases responsible for a considerable burden of disease. In this article, we reviewed the relationship between neutrophil extracellular traps (NETs) and chronic inflammatory airway diseases. DATA SOURCES: Articles published up to January 1, 2017, were selected from the PubMed, Ovid Medline, Embase databases, with the keywords of "asthma" or "pulmonary disease, chronic obstructive", "neutrophils" and "extracellular traps...
March 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28303208/surgical-strategy-to-minimize-ischemia-during-trapping-resection-of-giant-extracranial-carotid-artery-aneurysm-stratified-by-collateral-evaluation
#15
Hiroki Hongo, Tomohiro Inoue, Akira Tamura, Isamu Saito
BACKGROUND: Extracranial carotid artery aneurysm (ECAA) is a rare clinical entity, and no standard treatment strategy has been established for this condition. METHODS: Data from three patients who underwent surgical treatment of enlarging giant ECAAs were retrospectively reviewed. Based on the collateral status, as evaluated by preoperative digital subtraction angiography (DSA), surgical strategy was stratified into (1) high flow bypass followed by cervical ICA (internal carotid artery) ligation, when the collateral status was judged as poor/fair or (2) direct cervical repair with patch application after aneurysmal wall resection when the collateral status was judged as robust...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28303140/netosis-in-alzheimer-s-disease
#16
REVIEW
Enrica Caterina Pietronigro, Vittorina Della Bianca, Elena Zenaro, Gabriela Constantin
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the progressive deterioration of cognitive functions. Its neuropathological features include amyloid-β (Aβ) accumulation, the formation of neurofibrillary tangles, and the loss of neurons and synapses. Neuroinflammation is a well-established feature of AD pathogenesis, and a better understanding of its mechanisms could facilitate the development of new therapeutic approaches. Recent studies in transgenic mouse models of AD have shown that neutrophils adhere to blood vessels and migrate inside the parenchyma...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28299930/cross-dehydrogenative-coupling-of-heterocyclic-scaffolds-with-unfunctionalised-aroyl-surrogates-by-palladium-ii-catalyzed-c-sp2-h-aroylation-through-organocatalytic-dioxygen-activation
#17
Bhavin Viththlbhai Pipaliya, Asit K Chakraborti
Cross dehydrogenative coupling of bio-relevant heterocyclic with arylmethanes for aroylation during the Pd(II)-catalysed C(sp2)-H activation has been achieved through dioxygen activation by NHPI. Mass spectrometry and 1H NMR based kinetic isotope effect studies revealed C-H bond activation as the rate determining step. Radical scavenging experiments indicated radical pathway. The 1H NMR of an aliquot of reaction mixture and in situ trapping with 2-aminothiophenol revealed formation of aldehyde during aerobic oxidation of the arylmethanes...
March 16, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28299713/isobar-separation-in-a-multiple-reflection-time-of-flight-mass-spectrometer-by-mass-selective-re-trapping
#18
Timo Dickel, Wolfgang R Plaß, Wayne Lippert, Johannes Lang, Mikhail I Yavor, Hans Geissel, Christoph Scheidenberger
A novel method for (ultra-)high-resolution spatial mass separation in time-of-flight mass spectrometers is presented. Ions are injected into a time-of-flight analyzer from a radio frequency (rf) trap, dispersed in time-of-flight according to their mass-to-charge ratios and then re-trapped dynamically in the same rf trap. This re-trapping technique is highly mass-selective and after sufficiently long flight times can provide even isobaric separation. A theoretical treatment of the method is presented and the conditions for optimum performance of the method are derived...
March 15, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28296393/mechanism-of-reduction-of-ferric-porphyrins-by-sulfide-identification-of-a-low-spin-fe-iii-sh-intermediate
#19
Kaustuv Mittra, Asmita Singha, Abhishek Dey
The reaction of Fe(III) porphyrin complexes bearing distal hydrogen bonding residues with sulfide/hydrosulfide is kinetically monitored to reveal the presence of an intermediate and a kH/kD of 3.0. This intermediate is trapped at low temperatures and investigated with resonance Raman and electron paramagnetic resonance spectroscopy. The results, corroborated by density functional theory calculations, indicate that this species is a six-coordinate low spin hydrosulfide bound ferric porphyrin. The homolytic cleavage of the Fe(III)-SH bond resulting in the formation of a ferrous porphyrin and hydrosulfide radical (trapped with 5,5-dimethyl-1-pyrrilone-N-oxide) is found to be the overall rate-determining step of the reaction...
March 15, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28296180/antioxidant-capacity-phenolic-constituents-and-toxicity-of-hot-water-extract-from-red-maple-buds
#20
Naamwin R Meda, Patrice E Poubelle, Tatjana Stevanovic
The present study reports, for the first time, the results of the antioxidant capacity and the phenolic composition of a hot water extract from red maple buds (RMB), as well as its safety. In this regard and comparatively to antioxidant standards, this extract exhibits a significant antiradical capacity when tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and anion superoxide trapping assays. High-resolution mass spectrometric (HRMS) and Nuclear Magnetic Resonance (NMR) analyses permitted to determine for the first time, in red maple species, cyanidin-3-O-glucoside, quercetin-3-O-galactoside, quercetin-3-O-arabinoside and quercetin...
March 11, 2017: Chemistry & Biodiversity
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