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https://www.readbyqxmd.com/read/29732104/dual-channel-fluorescence-diagnosis-of-cancer-cells-tissues-assisted-by-oatp-transporters-and-cysteine-glutathione
#1
Hongxing Zhang, Jing Liu, Bo Hu, Linfang Wang, Zhen Yang, Xu Han, Juanjuan Wang, Wei Bai, Wei Guo
Although fluorescence imaging diagnosis of the differences between cancer cells and normal cells by targeting ligand-based fluorescent probes is useful for recommending personalized therapy to patients, using the differences to diagnose a wide range of cancers is often not possible due to the genetic or phenotypic heterogeneity of cancer cells. In this work a 2-(diphenylphosphino)phenol-functionalized pyronin POP was presented as a dual-channel fluorescence agent for diagnosing a wide range of cancer cell types...
March 28, 2018: Chemical Science
https://www.readbyqxmd.com/read/29729735/three-dimensional-porous-self-assembled-chestnut-like-nickel-cobalt-oxide-structure-as-an-electrochemical-sensor-for-sensitive-detection-of-hydrazine-in-water-samples
#2
Xiaohui Zhang, Yanfeng Wang, Xingming Ning, Linfang Li, Jing Chen, Duoliang Shan, Ruiqin Gao, Xiaoquan Lu
Three-dimensional NiCo2 O4 is a kind of superior sensing material owing to its high electron transfer capability, large available surface area and numbers of active sites. In this work, NiCo2 O4 of the three-dimensional chestnut-like structure were easily achieved through a one step hydrothermal process. Afterwards, the morphology and structure were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS)...
August 31, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29609043/evaluation-of-he4-and-ttr-for-diagnosis-of-ovarian-cancer-comparison-with-ca-125
#3
Xin Zheng, Shulin Chen, Linfang Li, Xiaohua Liu, Xiaomin Liu, Shuqin Dai, Peng Zhang, Huaiwu Lu, Zhongqiu Lin, Yuefei Yu, Guorong Li
OBJECTIVE: Serum human epididymis protein 4 (HE4) and transthyretin (TTR) are new markers for ovarian cancer. We compared HE4 and TTR with the gold marker CA-125 for the diagnosis of ovarian cancer patients. METHODS: 130 serum samples from benign ovarian tumor and 400 serum samples from healthy women were used to set up the cut-off. 126 serum samples from ovarian cancer patients before operation were collected to test the diagnostic value of these ELISA assays. The sensitivity, positive predictive value (PPV) and ROC curves were used to evaluate the diagnostic value...
March 30, 2018: Journal of Gynecology Obstetrics and Human Reproduction
https://www.readbyqxmd.com/read/29593773/sequencing-and-analysis-of-strobilanthes-cusia-nees-kuntze-chloroplast-genome-revealed-the-rare-simultaneous-contraction-and-expansion-of-the-inverted-repeat-region-in-angiosperm
#4
Haimei Chen, Junjie Shao, Hui Zhang, Mei Jiang, Linfang Huang, Zhao Zhang, Dan Yang, Molly He, Mostafa Ronaghi, Xi Luo, Botao Sun, Wuwei Wu, Chang Liu
Ban-Lan-Gen, the root tissues derived from several morphologically indistinguishable plant species, have been used widely in traditional Chinese medicines for numerous years. The identification of reliable markers to distinguish various source plant species is critical for the effective and safe use of products containing Ban-Lan-Gen. Here, we analyzed and characterized the complete chloroplast (cp) genome sequence of Strobilanthes cusia (Nees) Kuntze to identify high-resolution markers for the species determination of Southern Ban-Lan-Gen...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29550097/effect-of-somatostatin-on-prevention-of-post-endoscopic-retrograde-cholangiopancreatography-pancreatitis-and-hyperamylasemia-a-systematic-review-and-meta-analysis
#5
REVIEW
Guiliang Wang, Gui Xiao, Linfang Xu, Ping Qiu, Ting Li, Xiaoli Wang, Ping Wen, Jianbo Wen, Xianzhong Xiao
OBJECTIVE: To perform a meta-analysis of all available studies on the effect of prophylactic somatostatin administration on prevention of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) and post-ERCP hyperamylasemia (PEHA). METHODS: Electronic databases, including PubMed, EMBASE, the Cochrane library, and the Science Citation Index were searched to retrieve relevant trials. Randomized, placebo-controlled trials in adult patients that compared somatostatin versus placebo in prevention of PEP were included...
March 10, 2018: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/29548344/correction-to-rhbdd1-promotes-colorectal-cancer-metastasis-through-the-wnt-signaling-pathway-and-its-downstream-target-zeb1
#6
Mengmeng Zhang, Fei Miao, Rong Huang, Wenjie Liu, Yuechao Zhao, Tao Jiao, Yalan Lu, Fan Wu, Xiaojuan Wang, Han Wang, Hong Zhao, Hongge Ju, Shiying Miao, Linfang Wang, Wei Song
In the publication of this article [1], there is an error in the Methods section at paragraph Cell culture and reagents and the Additional file2: Fig. S1 was erroneous linked.
March 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29506383/bimodal-electrochemiluminescence-of-g-cnqds-in-the-presence-of-double-coreactants-for-ascorbic-acid-detection
#7
Huan Wang, Guiqiang Pu, Samrat Devaramani, Yanfeng Wang, Zhaofan Yang, Linfang Li, Xiaofang Ma, Xiaoquan Lu
How to improve the accuracy of target detection substance in low-content and complex of real sample, which is still a major challenge in the analysis field. There is no doubt that the internal standard method is the best choice in the analysis methods. The internal standard method of ECL strategy can furnish more accurate detection results in the changeable complex environment, and it can dispel the primary vaguest interference in the system through the self-calibration of two emission spectra. Herein, we effectually explored a strong and stable bimodal ECL system based on graphitic carbon nitride quantum dots (g-CNQDs) as single luminophore in the presence of double coreactants potassium persulfate (K2 S2 O8 ) and tetrabutylammonium bromide (TBAB) under the optimized conditions...
April 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29486557/dual-functions-of-lip6-and-its-regulation-of-lipid-metabolism-in-the-oleaginous-fungus-mucor-circinelloides
#8
Xinyi Zan, Xin Tang, Linfang Chu, Yuanda Song
Although multiple roles of lipases have been reported in yeasts and microalgae, the functions of lipases have not been studied in oleaginous filamentous fungi. Lipase Lip6 has been reported in the oleaginous filamentous fungus Mucor circinelloides with the consensus lipase motif GXSXG and the typical acyltransferase motif of H-(X)4 -D. To demonstrate that Lip6 might play dual roles as a lipase and an acyltransferase, we performed site-directed mutagenesis in the lipase motif and the acyltransferase motif of Lip6...
March 21, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29426364/rhbdd1-promotes-colorectal-cancer-metastasis-through-the-wnt-signaling-pathway-and-its-downstream-target-zeb1
#9
Mengmeng Zhang, Fei Miao, Rong Huang, Wenjie Liu, Yuechao Zhao, Tao Jiao, Yalan Lu, Fan Wu, Xiaojuan Wang, Han Wang, Hong Zhao, Hongge Ju, Shiying Miao, Linfang Wang, Wei Song
BACKGROUND: 40-50% of colorectal cancer (CRC) patients develop metastatic disease; the presence of metastasis hinders the effective treatment of cancer through surgery, chemotherapy and radiotherapy, which makes 5-year survival rate extremely low; therefore, studying CRC metastasis is crucial for disease therapy. In the present study, we investigated the role of rhomboid domain containing 1 (RHBDD1) in tumor metastasis of CRC. METHODS: The expression of RHBDD1 was analyzed in 539 colorectal tumor tissues for its correlation with lymphatic metastasis and distal metastasis...
February 9, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29414090/a-novel-dual-potential-ratiometric-electrochemiluminescence-dna-sensor-based-on-enhancing-and-quenching-effect-by-g-quadruplex-hemin-and-au-luminol-bifunctional-nanoparticles
#10
Yanfeng Wang, Duoliang Shan, Guofan Wu, Huan Wang, Fan Ru, Xiaohui Zhang, Linfang Li, Yaxuan Qian, Xiaoquan Lu
An ultrasensitive and stable "dual-potential" ratiometric electrochemiluminescence (ECL) sensor is reported for specific DNA, the femtomolar detection limit (0.12 fM, S/N = 3) and high selectivity insure its potential applications in cancer biomarkers searching or monitoring. The excellent performance of the sensor comes from simultaneously fabricated layer by layer structure "target DNA + Hemin / Au-Luminol NPs / DNA* / sl DNA / TGA / QDs / MWNTs / GCE" mode which was based on the enhancing effect of luminol by G-quadruplex / hemin and Au nanoparticles and the quenching effect of CdSe/ZnS by G-quadruplex / hemin...
May 30, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29410394/silencing-ras-related-c3-botulinum-toxin-substrate-1-inhibits-growth-and-migration-of-hypopharyngeal-squamous-cell-carcinoma-via-the-p38-mitogen-activated-protein-kinase-signaling-pathway
#11
Huijuan Cheng, Weiwei Wang, Guangke Wang, Anran Wang, Linfang Du, Weihua Lou
BACKGROUND Ras-related C3 botulinum toxin substrate 1 (Rac1) is implicated in a variety of cellular functions and is related to tumor growth and metastasis. This study aimed to explore the role of Rac1 in hypopharyngeal squamous cell carcinoma (HSCC). MATERIAL AND METHODS The Rac1 expression in HSCC tissues was determined by quantitative real-time polymerase chain reaction and Western blot analysis. The level of Rac1 in HSCC cells was downregulated by a Rac1-specific shRNA. Then, the growth and metastasis of HSCC cells were assessed in vitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, flow cytometry, Hoechst staining, and Transwell assay...
February 7, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29344274/immunotherapy-of-patient-with-hepatocellular-carcinoma-using-cytotoxic-t-lymphocytes-ex-vivo-activated-with-tumor-antigen-pulsed-dendritic-cells
#12
Ying Wang, Xijing Yang, Yi Yu, Zenghui Xu, Yan Sun, Hui Liu, Jingbo Cheng, Min Liu, Bibo Sha, Linfang Li, Na Ding, Zhong Li, Huajun Jin, Qijun Qian
Purpose The aim of this study was to evaluate the clinical response of immunotherapy with dendritic cell-cytotoxic T lymphocytes (DC-CTLs) in patients with hepatocellular carcinoma (HCC). Method Sixty-eight patients with a confirmed diagnosis of HCC and who received follow-up until December 2015 were enrolled. We measured immune phenotypes of DCs and activated T cells using flow cytometry and clinical indexes using an electrochemiluminescence method. Results DCs exhibited up-regulation of the maturation markers CD83, CD80, CD11c, and CD86 on day8...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29218012/anti-tumor-effect-of-rhaponticum-uniflorum-ethyl-acetate-extract-by-regulation-of-peroxiredoxin1-and-epithelial-to-mesenchymal-transition-in-oral-cancer
#13
Hui Chen, Chunxiao Wang, Moci Qi, Lihua Ge, Zhenchuan Tian, Jinhua Li, Min Zhang, Min Wang, Linfang Huang, Xiaofei Tang
Objective: To explore whether Rhaponticum uniflorum ( R. uniflorum ) had anti-tumor effects in oral cancer and investigate the molecular mechanisms involved in these anti-tumor effects. Methods: Chemical compositions of R. uniflorum ethyl acetate (RUEA) extracts were detected by ultra-performance liquid chromatography-Q/time-of-flight mass spectrometry (UPLC-Q/TOF-MS), followed by pharmacology-based network prediction analysis. The effects of RUEA extracts on proliferation, apoptosis, migration, and invasion ability of human oral squamous cell carcinoma (OSCC) cell line SCC15 were evaluated by CCK8 assay, Annexin V- fluorescein isothiocyanate/propidium iodide staining, wound healing assay, and Matrigel invasion assay, respectively...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29210380/construction-of-magnetically-separable-nial-ldh-fe-3-o-4-rgo-nanocomposites-with-enhanced-photocatalytic-performance-under-visible-light
#14
Jie Ni, Jinjuan Xue, LinFang Xie, Jing Shen, Guangyu He, Haiqun Chen
Magnetic NiAl layered doubled hydroxide (LDH)/Fe3 O4 -RGO composites were successfully synthesized via a simple hydrothermal route. The as-prepared samples were well characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The results showed that NiAl LDH nanoplatelets and Fe3 O4 nanoparticles sized around 15 nm were uniformly anchored on the surface of graphene sheets. The NiAl LDH/Fe3 O4 -RGO25 photocatalyst was employed to degrade ciprofloxacin (CIP) in an aqueous solution under visible light irradiation...
December 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29196641/simultaneous-realization-of-high-sensing-sensitivity-and-tunability-in-plasmonic-nanostructures-arrays
#15
Yuan-Fong Chou Chau, Chan-Kuang Wang, Linfang Shen, Chee Ming Lim, Hai-Pang Chiang, Chung-Ting Chou Chao, Hung Ji Huang, Chun-Ting Lin, N T R N Kumara, Nyuk Yoong Voo
A plasmonic nanostructure (PNS) which integrates metallic and dielectric media within a single structure has been shown to exhibit specific plasmonic properties which are considered useful in refractive index (RI) sensor applications. In this paper, the simultaneous realization of sensitivity and tunability of the optical properties of PNSs consisting of alternative Ag and dielectric of nanosphere/nanorod array have been proposed and compared by using three-dimensional finite element method. The proposed system can support plasmonic hybrid modes and the localized surface plasmonic resonances and cavity plasmonic resonances within the individual PNS can be excited by the incident light...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29190879/longevity-of-protective-immune-responses-induced-by-a-split-influenza-a-h7n9-vaccine-mixed-with-mf59-adjuvant-in-balb-c-mice
#16
Huilin Ou, Wei Yao, Dongshan Yu, Tianhao Weng, Frederick X C Wang, Xiaoxin Wu, Haibo Wu, Linfang Cheng, Xiangyun Lu, Nanping Wu, Honglin Chen, Lanjuan Li, Hangping Yao
The influenza virus is a serious threat to public health worldwide. A novel avian influenza A (H7N9) virus with a mortality rate of approximately 30% has been identified as an unusually dangerous virus for humans by the World Health Organization. Pathogenic H7N9 continue to represent a public health concern, and several candidate vaccines are currently in development. We generated candidate H7N9 vaccine strains using reverse genetics, consisting of hemagglutinin and neuraminidase genes derived from a human H7N9 virus and the remaining genes from the PR8 (A/PuertoRico/8/34 (H1N1)) virus...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29182593/analytical-methods-of-phytochemicals-from-the-genus-gentiana
#17
REVIEW
Yan Xu, Ying Li, Katherine G Maffucci, Linfang Huang, Rui Zeng
The genus Gentiana comprises approximately 400 species. Many species have a wide range of pharmacological activities and have been used therapeutically for thousands of years. To provide comprehensive guidance, utilization and quality control of Gentiana species, this review presents updated information concerning the recent application and progress of chemical analysis including phytochemical analysis, sample preparation and chemometrics. Detailed and comprehensive data including number of analytes, extraction/separation methods, analytical techniques and chemometrics are shown as corresponding tables...
November 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29171118/translesional-pressure-gradient-and-leptomeningeal-collateral-status-in-symptomatic-middle-cerebral-artery-stenosis
#18
X Leng, L Lan, H L Ip, F Fan, S H Ma, K Ma, H Liu, Z Yan, J Liu, J Abrigo, Y O Y Soo, D S Liebeskind, K S Wong, T W Leung
BACKGROUND AND PURPOSE: Leptomeningeal collateral (LMC) status governs the prognosis of large artery occlusive stroke, although factors determining LMC status are not fully elucidated. The aim was to investigate metrics affecting LMC status in such patients by using computational fluid dynamics (CFD) models based on computed tomography angiography (CTA). METHODS: In this cross-sectional study, patients with recent ischaemic stroke or transient ischaemic attack attributed to atherosclerotic M1 middle cerebral artery (MCA) stenosis (50%-99%) were recruited...
February 2018: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/29156573/neuraminidase-inhibitory-activity-and-constituent-characterization-of-fagopyrum-dibotrys
#19
Xiang Zhang, Yu Cao, Jinhua Li, Ailin Liu, Haibo Liu, Linfang Huang
This study aimed to identify a new biological activity of the widely distributed species Fagopyrum dibotrys. Four F. dibotrys extracts (ethyl acetate (EA), petroleum ether (P), ethanol (E), and water (W)) were explored for their anti-neuraminidase (NA) activity. A total of 32 compounds were identified using UHPLC-Q-Exactive Orbitrap HRMS in the EA extract, which had the best NA inhibitory effects. We used the docking data for supporting compounds' anti-neuraminidase activity. Among them, five compounds including one flavonoid, three organic acids, and one glucoside were discovered for the first time in F...
November 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29146988/variations-in-nutrient-and-trace-element-composition-of-rice-in-an-organic-rice-frog-coculture-system
#20
Zhimin Sha, Qingnan Chu, Zheng Zhao, Yubo Yue, Linfang Lu, Jing Yuan, Linkui Cao
Introducing frogs into paddy fields can control pests and diseases, and organic farming can improve soil fertility and rice growth. The aim of this 2-year field study was compare the yield and elemental composition of rice between an organic farming system including frogs (ORF) and a conventional rice culture system (CR). The grain yields were almost the same in the ORF system and the CR system. The ORF significantly increased the contents of phosphorus (P), ion (Fe), zinc (Zn), molybdenum (Mo) and selenium (Se) in rice grain at one or both years...
November 16, 2017: Scientific Reports
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