keyword
MENU ▼
Read by QxMD icon Read
search

Antigenic drift

keyword
https://www.readbyqxmd.com/read/28642154/a-boosting-skin-vaccination-with-dissolving-microneedle-patch-encapsulating-m2e-vaccine-broadens-the-protective-efficacy-of-conventional-influenza-vaccines
#1
Wandi Zhu, Winston Pewin, Chao Wang, Yuan Luo, Gilbert X Gonzalez, Teena Mohan, Mark R Prausnitz, Bao-Zhong Wang
The biodegradable microneedle patch (MNP) is a novel technology for vaccine delivery that could improve the immunogenicity of vaccines. To broaden the protective efficiency of conventional influenza vaccines, a new 4M2e-tFliC fusion protein construct containing M2e sequences from different subtypes was generated. Purified fusion protein was encapsulated into MNPs with a biocompatible polymer for use as a boosting vaccine. The results demonstrated that mice receiving a conventional inactivated vaccine followed by a skin-applied dissolving 4M2e-tFliC MNP boost could better maintain the humoral antibody response than that by the conventional vaccine-prime alone...
June 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28637337/matrix-completion-with-side-information-and-its-applications-in-predicting-the-antigenicity-of-influenza-viruses
#2
Li Huang, Xianhong Li, Pengfei Guo, Yuhua Yao, Bo Liao, Weiwei Zhang, Fayou Wang, Jiasheng Yang, Yulong Zhao, Hailiang Sun, Pingan He, Jialiang Yang
Motivation: Low-rank matrix completion has been demonstrated to be powerful in predicting antigenic distances among influenza viruses and vaccines from partially revealed hemagglutination inhibition (HI) table. Meanwhile, influenza hemagglutinin (HA) protein sequences are also effective in inferring antigenic distances. Thus, it is natural to integrate HA protein sequence information into low-rank matrix completion model to help infer influenza antigenicity, which is critical to influenza vaccine development...
June 16, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28631598/in-vitro-and-in-vivo-evidence-of-a-potential-a-h1n1-pdm09-antigenic-drift-mediated-by-escape-mutations-in-the-haemagglutinin-sa-antigenic-site
#3
Miguel Retamal, Yacine Abed, Chantal Rhéaume, Mariana Baz, Guy Boivin
Influenza A(H1N1)pdm09 virus continues to circulate worldwide without evidence of significant antigenic drift between 2009 and 2016. By using escape mutants, we previously identified six haemagglutinin (HA) changes (T80R, G143E, G158E, N159D, K166E and A198E) that were located within antigenic sites. Combinations of these mutations were introduced into the A(H1N1)pdm09 HA plasmid by mutagenesis. Reassortant 6 : 2 viruses containing both the HA and NA genes of the A(H1N1)pdm09 and the six internal gene segments of A/PR/8/34 were rescued by reverse genetics...
June 20, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28619136/coimmunization-with-recombinant-epitope-expressing-baculovirus-enhances-protective-effects-of-inactivated-h5n1-vaccine-against-heterologous-virus
#4
Zhen Zhang, Jiao Zhang, Jie Zhang, Qi Li, Peisi Miao, Jie Liu, Shijie Li, Jianni Huang, Ming Liao, Huiying Fan
H5N1, a highly pathogenic avian influenza virus (HPAIV), poses a significant threat to poultry and human health. However, currently available inactivated influenza vaccines are less efficacious against viruses that display antigenic drift. In this study, we constructed a recombinant baculovirus (BV-HMNN) expressing four conserved antigen epitopes: H5N1 hemagglutinin stem area amino acids 76-130 (HA2 76-130); three tandem repeats from the ectodomain of the conserved influenza matrix protein M2 (3M2e); nucleoprotein amino acids 55-69 (NP55-69); and nucleoprotein amino acids 380-393 (NP380-393)...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28616748/intact-transition-epitope-mapping-item
#5
Yelena Yefremova, Kwabena F M Opuni, Bright D Danquah, Hans-Juergen Thiesen, Michael O Glocker
Intact transition epitope mapping (ITEM) enables rapid and accurate determination of protein antigen-derived epitopes by either epitope extraction or epitope excision. Upon formation of the antigen peptide-containing immune complex in solution, the entire mixture is electrosprayed to translate all constituents as protonated ions into the gas phase. There, ions from antibody-peptide complexes are separated from unbound peptide ions according to their masses, charges, and shapes either by ion mobility drift or by quadrupole ion filtering...
June 14, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28601563/biophysical-characterization-of-influenza-a-virions
#6
Arun Parupudi, Flaviu Gruia, Samuel A Korman, Sonia Dragulin-Otto, Kuldip Sra, Richard L Remmele, Jared S Bee
Antigenic drift of the influenza A virus requires that vaccine production is targeted to the strains circulating each year. Live-attenuated influenza A vaccine manufacturing is used to produce intact virions with the surface antigens of the circulating strains. Influenza A typically contains a large percentage (>90%) of non-infective virions. The ribonucleoprotein (RNP) content, virion structure, and aggregation are factors that are thought to have an impact on infectivity. However, these factors are difficult to study because of the intrinsic variability in virion size, shape and overall structural integrity...
June 8, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28588131/rationally-designed-influenza-virus-vaccines-that-are-antigenically-stable-during-growth-in-eggs
#7
Alfred T Harding, Brook E Heaton, Rebekah E Dumm, Nicholas S Heaton
Influenza virus vaccine production is currently limited by the ability to grow circulating human strains in chicken eggs or in cell culture. To facilitate cost-effective growth, vaccine strains are serially passaged under production conditions, which frequently results in mutations of the major antigenic protein, the viral hemagglutinin (HA). Human vaccination with an antigenically drifted strain is known to contribute to poor vaccine efficacy. To address this problem, we developed a replication-competent influenza A virus (IAV) with an artificial genomic organization that allowed the incorporation of two independent and functional HA proteins with different growth requirements onto the same virion...
June 6, 2017: MBio
https://www.readbyqxmd.com/read/28587867/spatiotemporal-dynamics-of-influenza-a-h1n1-pdm09-in-brazil-during-the-pandemic-and-post-pandemic-periods
#8
Alessandra C B Manito, Tiago Gräf, Vagner R Lunge, Nilo Ikuta
Influenza A(H1N1)pdm09 was responsible for the first global flu pandemic in 21st century affecting all the world. In Brazil, A(H1N1)pdm09 is still circulating as a seasonal virus, causing deaths every year. Nevertheless, the viral diffusion process that yearly seeds new influenza strains in the country was not investigated yet. The aim of the current study was to describe the phylodynamics and phylogeography of influenza A(H1N1)pdm09 in Brazil between 2009 and 2014. Neuraminidase sequences from Brazil and other regions of the World were retrieved and analyzed...
June 3, 2017: Virus Research
https://www.readbyqxmd.com/read/28559489/in-vivo-imaging-of-influenza-virus-infection-in-immunized-mice
#9
Rita Czakó, Leatrice Vogel, Elaine W Lamirande, Kevin W Bock, Ian N Moore, Ali H Ellebedy, Rafi Ahmed, Andrew Mehle, Kanta Subbarao
Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical efficacy of candidate vaccines and immunotherapy in a mouse model of influenza. Sequential imaging revealed distinct spatiotemporal kinetics of bioluminescence in groups of mice passively or actively immunized by various strategies that accelerated the clearance of the challenge virus at different rates and by distinct mechanisms...
May 30, 2017: MBio
https://www.readbyqxmd.com/read/28542275/combination-of-the-immunization-with-the-sequence-close-to-the-consensus-sequence-and-two-dna-prime-plus-one-vlp-boost-generate-h5-hemagglutinin-specific-broad-neutralizing-antibodies
#10
Guiqin Wang, Renfu Yin, Paul Zhou, Zhuang Ding
Hemagglutinin (HA) head has long been considered to be able to elicit only a narrow, strain-specific antibody response as it undergoes rapid antigenic drift. However, we previously showed that a heterologous prime-boost strategy, in which mice were primed twice with DNA encoding HA and boosted once with virus-like particles (VLP) from an H5N1 strain A/Thailand/1(KAN)-1/2004 (noted as TH DDV), induced anti-head broad cross-H5 neutralizing antibody response. To explain why TH DDV immunization could generate such breadth, we systemically compared the neutralization breadth and potency between TH DDV sera and immune sera elicited by TH DDD (three times of DNA immunizations), TH VVV (three times of VLP immunizations), TH DV (one DNA prime plus one VLP boost) and TK DDV (plasmid DNA and VLP derived from another H5N1 strain, A/Turkey/65596/2006)...
2017: PloS One
https://www.readbyqxmd.com/read/28493054/the-dynamics-of-a-seir-sirc-antigenic-drift-influenza-model
#11
Fajar Adi-Kusumo
We consider the dynamics of an influenza model with antigenic drift mechanism. Antigenic drift is an antigen mutation on the skin surface of the influenza virus that do not produce a new virus strain. The mutation produces the same virus but with slightly different antigens that cannot be recognized by the immune receptors formed by the previous infection. There are some type of influenza that involve the interaction between two populations such as human and animal. In this paper, we construct an influenza model with antigenic drift mechanism on the human population that has interaction with the animal population...
May 10, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28484283/predicting-influenza-antigenicity-from-hemagglutintin-sequence-data-based-on-a-joint-random-forest-method
#12
Yuhua Yao, Xianhong Li, Bo Liao, Li Huang, Pingan He, Fayou Wang, Jiasheng Yang, Hailiang Sun, Yulong Zhao, Jialiang Yang
Timely identification of emerging antigenic variants is critical to influenza vaccine design. The accuracy of a sequence-based antigenic prediction method relies on the choice of amino acids substitution matrices. In this study, we first compared a comprehensive 95 substitution matrices reflecting various amino acids properties in predicting the antigenicity of influenza viruses by a random forest model. We then proposed a novel algorithm called joint random forest regression (JRFR) to jointly consider top substitution matrices...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28475122/harnessing-local-immunity-for-an-effective-universal-swine-influenza-vaccine
#13
REVIEW
Elma Tchilian, Barbara Holzer
Influenza A virus infections are a global health threat to humans and are endemic in pigs, contributing to decreased weight gain and suboptimal reproductive performance. Pigs are also a source of new viruses of mixed swine, avian, and human origin, potentially capable of initiating human pandemics. Current inactivated vaccines induce neutralising antibody against the immunising strain but rapid escape occurs through antigenic drift of the surface glycoproteins. However, it is known that prior infection provides a degree of cross-protective immunity mediated by cellular immune mechanisms directed at the more conserved internal viral proteins...
May 5, 2017: Viruses
https://www.readbyqxmd.com/read/28468875/immune-escape-variants-of-h9n2-influenza-viruses-containing-deletions-at-the-haemagglutinin-receptor-binding-site-retain-fitness-in-vivo-and-display-enhanced-zoonotic-characteristics
#14
Thomas P Peacock, Donald J Benton, Joe James, Jean-Remy Sadeyen, Pengxiang Chang, Joshua E Sealy, Juliet E Bryant, Stephen R Martin, Holly Shelton, Wendy S Barclay, Munir Iqbal
H9N2 avian influenza viruses are enzootic in poultry across Asia and North Africa where they pose a threat to human health, both as zoonotic agents and as potential pandemic candidates. Poultry vaccination against H9N2 viruses has been employed in many regions, however vaccine effectiveness is frequently compromised due to antigenic drift arising from amino acid substitutions in the major influenza antigen, haemagglutinin (HA). Using selection with HA specific monoclonal antibodies, we previously identified H9N2 antibody escape mutants that contained deletions of amino acids in the 220 loop of the HA receptor binding sites (RBS)...
May 3, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28443077/molecular-dynamics-simulation-of-the-influenza-a-h3n2-hemagglutinin-trimer-reveals-the-structural-basis-for-adaptive-evolution-of-the-recent-epidemic-clade-3c-2a
#15
Masaru Yokoyama, Seiichiro Fujisaki, Masayuki Shirakura, Shinji Watanabe, Takato Odagiri, Kimito Ito, Hironori Sato
Influenza A(H3N2) has been a major cause of seasonal influenza in humans since 1968, and has evolved by antigenic drift under the constantly changing human herd immunity. Increasing evidence suggests that the antigenic change occasionally occurred concomitant with the alterations of the N-glycosylation site profile and hemagglutination activity of the virion surface protein hemagglutinin (HA). However, the structural basis of these changes remains largely unclear. To address this issue, we performed molecular dynamics simulations of the glycosylated HA trimers of the A(H3N2), which has a novel pattern of Asn-X-Ser/Thr sequons unique in the new A(H3N2) epidemic clade 3C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28408121/effectiveness-of-live-attenuated-influenza-vaccine-and-inactivated-influenza-vaccine-in-children-during-the-2014-2015-season
#16
Huong Q McLean, Herve Caspard, Marie R Griffin, Katherine A Poehling, Manjusha Gaglani, Edward A Belongia, H Keipp Talbot, Timothy R Peters, Kempapura Murthy, Christopher S Ambrose
BACKGROUND: A clinical study found that live attenuated influenza vaccine (LAIV) was superior to inactivated influenza vaccine (IIV) against drifted A(H3N2) viruses in children. During the 2014-2015 influenza season, widespread circulation of antigenically and genetically drifted A(H3N2) viruses provided an opportunity to evaluate subtype-specific vaccine effectiveness (VE) of quadrivalent LAIV (LAIV4) and IIV in children. METHODS: Children (2-17years) with febrile acute respiratory illness <5days' duration were enrolled at 4 outpatient sites in the United States during the 2014-2015 influenza season...
May 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28401407/molecular-analysis-of-amantadine-resistant-influenza-a-h1n1-pdm09-virus-isolated-from-slum-dwellers-of-dhaka-bangladesh
#17
Mozibur Rahman, Sheikh Ariful Hoque, Mohammad Ariful Islam, Sabita Rezwana Rahman
Influenza is a highly contagious viral infection associated with excessive hospitalizations and deaths throughout the world. Continuous antigenic shift and drift is not only responsible for this devastating effect of influenza but also causes ineffectiveness of antiviral drugs and vaccines. In this study, we investigated the effectiveness of ribavirin, oseltamivir, and amantadine drugs in vitro against nine influenza A isolates collected during June 2012-August 2013 from different slums in Dhaka city. The effectiveness of these drugs was determined by measuring the inhibition of virus-induced cytopathic effect on MDCK cells through MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)...
June 2017: Virus Genes
https://www.readbyqxmd.com/read/28370432/antigenic-diversity-of-h5-highly-pathogenic-avian-influenza-viruses-of-clade-2-3-4-4-isolated-in-asia
#18
Ayako Ohkawara, Masatoshi Okamatsu, Makoto Ozawa, Duc-Huy Chu, Lam Thanh Nguyen, Takahiro Hiono, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda
H5 highly pathogenic avian influenza viruses (HPAIVs) have spread in both poultry and wild birds since the late 2003. The continued circulation of HPAIVs in poultry in several regions of the world has led to antigenic drift. In this study, we analyzed the antigenic properties of H5 HPAIVs isolated in Asia using four neutralizing monoclonal antibodies (MAbs) recognizing the hemagglutinin, which were established using A/chicken/Kumamoto/1-7/2014 (H5N8), belonging to clade 2.3.4.4 and also using polyclonal antibodies...
March 30, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28356529/antigenic-drift-defines-a-new-d4-subgenotype-of-measles-virus
#19
Miguel Ángel Muñoz-Alía, Claude P Muller, Stephen J Russell
The measles virus hemagglutinin (MeV-H) protein is the main target of protective neutralizing antibodies. Using a panel of monoclonal antibodies (MAbs) that recognize known major antigenic sites in MeV-H, we identified a D4 genotype variant that escapes neutralization by MAbs targeting the neutralizing epitope (NE) antigenic site. By site-directed mutagenesis, L249P was identified as the critical mutation disrupting the NE in this genotype D4 variant. Forty-two available D4 genotype gene sequences were subsequently analyzed and divided into 2 groups according to the presence or absence of the L249P MeV-H mutation...
June 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28356526/chimeric-hemagglutinin-constructs-induce-broad-protection-against-influenza-b-virus-challenge-in-the-mouse-model
#20
Megan Ermler, Ericka Kirkpatrick, Weina Sun, Rong Hai, Fatima Amanat, Veronika Chromikova, Peter Palese, Florian Krammer
Seasonal influenza virus epidemics represent a significant public health burden. Approximately 25% of all influenza virus infections are caused by type B viruses and these infections can be severe, especially in children. Current influenza virus vaccines are an effective prophylaxis against infection but are impacted by rapid antigenic drift which can lead to mismatches between vaccine strains and circulating strains. Here, we describe a broadly protective vaccine candidate based on chimeric hemagglutinins consisting of globular head domains from exotic influenza A viruses and stalk domains from influenza B viruses...
March 29, 2017: Journal of Virology
keyword
keyword
42606
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"