keyword
MENU ▼
Read by QxMD icon Read
search

Antigen

keyword
https://www.readbyqxmd.com/read/28109183/chloride-ion-transport-and-overexpression-of-tmem16a-in-a-guinea-pig-asthma-model
#1
Mitsuko Kondo, Mayoko Tsuji, Kaori Hara, Ken Arimura, Osamitsu Yagi, Etsuko Tagaya, Kiyoshi Takeyama, Jun Tamaoki
BACKGROUND: TMEM16A, a Ca-activated Cl channel, regulates various physiological functions such as mucin secretion. However, the role of TMEM16A in hypersecretion in asthma is not fully understood. OBJECTIVE: The aim of this study is to evaluate Cl ion transport via TMEM16A and determine the localization of TMEM16A in a guinea pig asthma model. METHODS: Guinea pigs were sensitized with OVA ip. on Day 1 and Day 8. On Day 22, we assessed OVA challenge-induced Cl ion transport in the sensitized tracheas ex vivo in an Ussing chamber, compared with the non-sensitized tracheas...
January 21, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28109148/-rat-bone-marrow-mesenchymal-stem-cells-promote-apoptosis-and-inhibit-proliferation-and-migration-of-rsc96-cells
#2
Yongbin Ma, Suohua Ge, Dan Zhou, Huanyan Zhang, Luyang Sun, Xuefeng Wang, Jianhua Su
Objective To investigate the effect of rat bone marrow mesenchymal stem cells (BMSCs) on the proliferation and migration of rat RSC96 Schwann cells in vitro. Methods BMSCs of Sprague Dawley (SD) rats were isolated by bone marrow cell adherent method. The morphology of the third generation of BMSCs was observed by inverted microscopy. The cell surface antigen makers CD19, CD34, CD73, CD105 were detected by flow cytometry. The third-passage BMSCs differentiated into osteoblasts and adipocytes after exposed to bone-inducing and lipid-inducing media for 3 and 2 weeks, respectively, and then were identified by oil red O and alkaline phosphatase staining...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109139/-imiquimod-combined-with-dendritic-cell-vaccine-decreases-treg-proportion-and-enhances-anti-tumor-responses-in-mice-bearing-melanoma
#3
Shurong Ren, Qiubo Wang, Yanli Zhang, Cuixiu Lu, Ping Li, Yumei Li
Objective To investigate the therapeutic effect of Toll-like receptor 7 (TLR7) agonist imiquimod combined with dendritic cell (DC)-based tumor vaccine on melanoma in mice and the potential mechanism. Methods Melanoma-bearing mouse models were established by subcutanous injection of B16-OVA cells into C57BL/6 mice. DCs were isolated from mouse bone marrow and propagated in culture medium with recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant mouse interleukin-4 (rmIL-4)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109028/breadth-of-t-cell-responses-after-immunization-with-adenovirus-vectors-encoding-ancestral-antigens-or-polyvalent-papillomavirus-antigens
#4
Emeline Ragonnaud, Anders Gorm Pedersen, Peter Johannes Holst
Oncogenic human papillomaviruses (HPVs) are in most cases eliminated by intervention of T cells. As many other pathogens, these oncogenic HPVs belong to an ancient and diverse virus family. Therefore, we found it relevant to investigate the potential and limitations of inducing a broad response - either by inducing cross-reactive T cells or by administering a polyvalent vaccine. To test these strategies, we designed 3 ancestral and 2 circulating sequences based on the two domains of the E1 and E2 proteins of papillomaviruses (PVs) that exhibit the highest degree of conservation in comparison to the other PV proteins...
January 21, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28108992/intralesional-immunotherapy-compared-to-cryotherapy-in-the-treatment-of-warts
#5
Fahime Khozeimeh, Farahzad Jabbari Azad, Yaghoub Mahboubi Oskouei, Majid Jafari, Shahrzad Tehranian, Roohallah Alizadehsani, Pouran Layegh
BACKGROUND: Warts are the most common clinical manifestation of the human papilloma-virus infection in the skin and mucous membranes. In spite of the various therapeutic modalities for nongenital skin warts, there is still no single method to be used as an approved treatment. In this study, we compared the efficacy of immunotherapy and cryotherapy on wart lesions. METHODS: Sixty patients with verruca vulgaris and plantar warts were randomly divided into two groups...
January 21, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/28108991/mucosal-immune-tolerance-at-the-ocular-surface-in-health-and-disease
#6
REVIEW
Jeremías G Galletti, Mauricio Guzmán, Mirta N Giordano
The ocular surface is constantly exposed to environmental irritants, allergens and pathogens, against which it can mount a prompt immune response to preserve its integrity. But to avoid unnecessary inflammation, the ocular surface's mucosal immune system must also discriminate between harmless and potentially dangerous antigens, a seemingly complicated task. Despite its unique features, the ocular surface is a mucosal lining, and as such, it shares some homeostatic and pathophysiological mechanisms with other mucosal surfaces...
January 20, 2017: Immunology
https://www.readbyqxmd.com/read/28108937/glycyrrhizin-ameliorates-experimental-colitis-through-attenuating-interleukin-17-producing-t-cell-responses-via-regulating-antigen-presenting-cells
#7
Xiangyu Chen, Dai Fang, Lingyun Li, Liyong Chen, Qirui Li, Feili Gong, Min Fang
Glycyrrhizin, a component of Chinese medicine licorice root, has the ability to inhibit the functions of high-mobility group box 1 (HMGB1). While glycyrrhizin is known to have anti-inflammatory activities, the underlying mechanisms by which glycyrrhizin inhibits inflammation during the development of trinitrobenzenesulfonic acid (TNBS)-induced experimental colitis are not well understood. This study systemically examined the regulatory effects of glycyrrhizin on inflammatory response in TNBS-induced murine colitis and explored the potential mechanisms involved in this process...
January 21, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28108828/a-novel-biomarker-based-preoperative-prognostic-grading-system-for-predicting-survival-after-surgery-for-intrahepatic-cholangiocarcinoma
#8
Tomoaki Yoh, Satoru Seo, Etsuro Hatano, Kojiro Taura, Hiroaki Fuji, Yoshinobu Ikeno, Yukihiro Okuda, Kentaro Yasuchika, Toshimi Kaido, Hideaki Okajima, Shinji Uemoto
BACKGROUND: Although treatment strategies for intrahepatic cholangiocarcinoma (ICC) are shifting towards multidisciplinary approaches, preoperative radiographic methods for identifying patients requiring further therapy are unclear. This study was designed to establish a prognostic grading system using preoperatively available objective biomarkers. METHODS: A novel preoperative prognostic grading system for predicting survival after surgery for ICC was developed from multivariate analysis of 134 ICC patients who underwent surgery between 1996 and 2015 using preoperatively available biomarkers...
January 20, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/28108804/antigenic-and-genotypic-characterization-of-rabies-virus-isolated-from-bats-mammalia-chiroptera-from-municipalities-in-s%C3%A3-o-paulo-state-southeastern-brazil
#9
Benedito Donizete Menozzi, Rafael de Novaes Oliveira, Laís Moraes Paiz, Virgínia Bodelão Richini-Pereira, Helio Langoni
Bats have aroused growing attention in the public health sphere because they are considered the main reservoir of rabies virus (RABV) in the Americas, in places where canine rabies is under control. Antigenic and genetic studies of RABV isolates have been used to describe the epidemiological profile of rabies and to identify possible hosts/reservoirs for different epidemiological cycles. This study describes the antigenic and genotypic characterization of 19 RABV isolates from central nervous system samples of non-hematophagous bats (Mammalia: Chiroptera)...
January 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28108611/reversal-of-diabetes-in-nod-mice-by-clinical-grade-proinsulin-and-il-10-secreting-lactococcus-lactis-in-combination-with-low-dose-anti-cd3-depends-on-the-induction-of-foxp3-positive-t-cells
#10
Tatiana Takiishi, Dana Paulina Cook, Hannelie Korf, Guido Sebastiani, Francesca Mancarella, João Paulo Monteiro Carvalho Mori Cunha, Clive Wasserfall, Noelia Casares, Juan José Lasarte, Lothar Steidler, Pieter Rottiers, Francesco Dotta, Conny Gysemans, Chantal Mathieu
The introduction of β-cell autoantigens via the gut through Lactococcus lactis (L. lactis) has been demonstrated to be a promising approach for diabetes reversal in NOD mice. Here we show that a combination therapy of low-dose anti-CD3 with a clinical-grade self-containing L. lactis, appropriate for human application, secreting human proinsulin and interleukin-10, cured 66% of mice with new-onset diabetes, which is comparable to therapy results with plasmid-driven L. lactis Initial blood glucose concentrations (<350 mg/dL) and insulin autoantibody positivity were predictors of the stable reversal of hyperglycemia, and decline in insulin autoantibody positivity was an immune biomarker of therapeutic outcome...
February 2017: Diabetes
https://www.readbyqxmd.com/read/28108559/antigen-processing-in-the-endoplasmic-reticulum-is-monitored-by-semi-invariant-%C3%AE-%C3%AE-tcrs-specific-for-a-conserved-peptide-qa-1b-mhc-class-ib-ligand
#11
Jian Guan, Soo Jung Yang, Federico Gonzalez, Yuxin Yin, Nilabh Shastri
Ag processing in the endoplasmic reticulum (ER) by the ER aminopeptidase associated with Ag processing (ERAAP) is central to presentation of a normal peptide-MHC class I (MHC I) repertoire. Alternations in ERAAP function cause dramatic changes in the MHC I-presented peptides, which elicit potent immune responses. An unusual subset of CD8(+) T cells monitor normal Ag processing by responding to a highly conserved FL9 peptide that is presented by Qa-1(b), a nonclassical MHC Ib molecule (QFL) in ERAAP-deficient cells...
January 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28108486/regulation-of-the-immune-response-by-tgf-%C3%AE-from-conception-to-autoimmunity-and-infection
#12
Shomyseh Sanjabi, Soyoung A Oh, Ming O Li
Transforming growth factor β (TGF-β) is a pleiotropic cytokine involved in both suppressive and inflammatory immune responses. After 30 years of intense study, we have only begun to elucidate how TGF-β alters immunity under various conditions. Under steady-state conditions, TGF-β regulates thymic T-cell selection and maintains homeostasis of the naïve T-cell pool. TGF-β inhibits cytotoxic T lymphocyte (CTL), Th1-, and Th2-cell differentiation while promoting peripheral (p)Treg-, Th17-, Th9-, and Tfh-cell generation, and T-cell tissue residence in response to immune challenges...
January 20, 2017: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/28108419/regulation-of-protein-kinase-c-related-kinase-prk-signalling-by-the-tp%C3%AE-and-tp%C3%AE-isoforms-of-the-human-thromboxane-a2-receptor-implications-for-thromboxane-and-androgen-dependent-neoplastic-and-epigenetic-responses-in-prostate-cancer
#13
Aine G O'Sullivan, Eamon P Mulvaney, B Therese Kinsella
The prostanoid thromboxane (TX) A2 and its T Prostanoid receptor (the TP) are increasingly implicated in prostate cancer (PCa). Mechanistically, we recently discovered that both TPα and TPβ form functional signalling complexes with members of the protein kinase C-related kinase (PRK) family, AGC- kinases essential for the epigenetic regulation of androgen receptor (AR)-dependent transcription and promising therapeutic targets for treatment of castrate-resistant prostate cancer (CRPC). Critically, similar to androgens, activation of the PRKs through the TXA2/TP signalling axis induces phosphorylation of histone H3 at Thr11 (H3Thr11), a marker of androgen-induced chromatin remodelling and transcriptional activation, raising the possibility that TXA2-TP signalling can mimic and/or enhance AR-induced cellular changes even in the absence of circulating androgens such as in CRPC...
January 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28108401/constitutive-expression-and-characterization-of-a-surface-srs-ncsrs67-protein-of-neospora-caninum-with-no-orthologue-in-toxoplasma-gondii
#14
Marcos Alexandre Bezerra, Luiz Miguel Pereira, Aline Bononi, Carla Agostino Biella, Luciana Baroni, Leticia Pollo-Oliveira, Ana Patrícia Yatsuda
Neospora caninum is a parasite of the Apicomplexa phylum responsible for abortion and losses of fertility in cattle. As part of its intracellular cycle, the first interaction of the parasite with the target cell is performed with the surface proteins known as the SRS superfamily (Surface Antigen Glycoprotein - Related Sequences). SAG related or SRS proteins have been a target of intense research due to its immunodominant pattern, exhibiting potential as diagnostic and/or vaccine candidates. The aim of this study was the cloning, expression and characterization of the gene NcSRS67 of N...
January 17, 2017: Parasitology International
https://www.readbyqxmd.com/read/28108375/the-role-of-pattern-recognition-receptors-in-lung-sarcoidosis
#15
Esmaeil Mortaz, Ian M Adcock, Atefhe Abedini, Arda Kiani, Mehdi Kazempour-Dizaji, Masoud Movassaghi, Johan Garssen
Sarcoidosis is a granulomatous disorder of unknown etiology. Infection, genetic factors, autoimmunity and an aberrant innate immune system have been explored as potential causes of sarcoidosis. The etiology of sarcoidosis remains unknown, and it is thought that it might be caused by an infectious agent in a genetically predisposed, susceptible host. Inflammation results from recognition of evolutionarily conserved structures of pathogens (Pathogen-associated molecular patterns, PAMPs) and/or from reaction to tissue damage associated patterns (DAMPs) through recognition by a limited number of germ line-encoded pattern recognition receptors (PRRs)...
January 17, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28108192/epidemiology-and-pathophysiology-of-childhood-narcolepsy
#16
REVIEW
Thomas J Dye, Neepa Gurbani, Narong Simakajornboon
It is now recognized that there are two types of narcolepsy. Narcolepsy type I or Narcolepsy with cataplexy is caused by the loss of hypocretin or orexin neurons. Narcolepsy type II or narcolepsy without cataplexy has normal hypocretin and the etiology is unknown. Hypocretin is a neuropeptide produced by neurons in the lateral hypothalamus. Both genetic and environmental factors play a crucial role in the pathogenesis of narcolepsy. Most patients with narcolepsy type I and half of patients with narcolepsy type II carry HLA-DQB1*0602...
December 21, 2016: Paediatric Respiratory Reviews
https://www.readbyqxmd.com/read/28108183/evaluation-of-multiplex-assay-platforms-for-detection-of-influenza-hemagglutinin-subtype-specific-antibody-responses-influenza-ha-subtype-specific-antibody-detection-platform
#17
Zhu-Nan Li, Kimberly M Weber, Rebecca A Limmer, Bobbi J Horne, James Stevens, Joy Schwerzmann, Jens Wrammert, Megan McCausland, Andrew J Phipps, Kathy Hancock, Daniel B Jernigan, Min Levine, Jacqueline M Katz, Joseph D Miller
Influenza hemagglutination inhibition (HI) and virus microneutralization assays (MN) are widely used for seroprevalence studies. However, these assays have limited field portability and are difficult to fully automate for high throughput laboratory testing. To address these issues, three multiplex influenza subtype-specific antibody detection assays were developed using recombinant hemagglutinin antigens in combination with Chembio, Luminex(®), and ForteBio(®) platforms. Assay sensitivity, specificity, and subtype cross-reactivity were evaluated using a panel of well characterized human sera...
January 17, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28108174/experimental-infection-of-octopus-vulgaris-cuvier-1797-with-photobacterium-damsela-subsp-piscicida-immunohistochemical-tracking-of-antigen-and-tissue-responses
#18
Vasileios Bakopoulos, Daniella White, Michail-Aggelos Valsamidis, Feli Vasilaki
Adult common octopus individuals were intramuscularly infected with Photobacterium damsela subsp. piscicida in order to investigate if this species is sensitive to this common and important fish pathogen. The fate of the bacterial antigens and the tissue responses of Octopus vulgaris were studied employing immunohistochemical techniques. Strong reaction at the site of injection was evident from day 2 post-infection that continued until day 14. Great numbers of hemocytes that were attracted at the site of infection were involved in phagocytosis of bacteria...
January 17, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28108005/in-vitro-allogeneic-immune-cell-response-to-mesenchymal-stromal-cells-derived-from-human-adipose-in-patients-with-rheumatoid-arthritis
#19
P Usha Shalini, J V S Vidyasagar, Lakshmi Kumari Kona, Meenakshi Ponnana, Lakshmi Kiran Chelluri
We investigated the regulatory activity of human adipose-derived mesenchymal stromal cells (MSCs) (n=10) towards immune cells in a cohort of 84 rheumatoid arthritis (RA) patients, 36 apparently healthy controls. We co-cultured MSCs with lymphocyte subsets of T, B, and T regulatory cells (Tregs). Levels of the pro- and anti-inflammatory markers (tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), and interleukin-10 (IL-10)) were estimated in serum and co-culture supernatants. The study revealed a two-fold increase in the proportion of Tregs and an increased level of CD4(+)CD25(+)FoxP3...
January 12, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28107991/setting-up-the-cut-off-level-of-a-sensitive-barcode-lateral-flow-assay-with-magnetic-nanoparticles
#20
Vasily G Panferov, Irina V Safenkova, Anatoly V Zherdev, Boris B Dzantiev
A barcode lateral flow immunoassay (LFIA) based on magnetic nanoparticles with a controllable cut-off level was developed for the first time. The regulation of the cut-off levels was based on the changes in the position and concentration of three antibodies (two monoclonal and polyclonal) with different affinities (KD=4.22×10(-9), 9.67×10(-9), 1.05×10(-8) M), respectively. To obtain specific conjugates, monoclonal antibodies were covalently immobilized on the magnetic nanoparticles' surface. Potato virus X causing a reduction in the potato yield was used as a model polyvalent antigen...
March 1, 2017: Talanta
keyword
keyword
42603
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"