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https://www.readbyqxmd.com/read/28240002/cholesterol-homeostasis-in-isolated-lymphocytes-a-differential-correlation-between-male-control-and-chronic-lymphocytic-leukemia-subjects
#1
Shrimanjunath Sankanagoudar, Govind Singh, Manaranjan Mahapatra, Lalit Kumar, Nimai Chand Chandra
Background: This study was performed to investigate any association between cellular cholesterol homeostasis and chronic lymphocytic leukemia (CLL). CLL is characterized primarily by an abnormal accumulation of neoplastic B cells in the blood, bone marrow, lymph nodes and spleen. Methods: Men aged >50 years participated in this study. Enzyme-based plasma lipid profile estimations, peripheral blood lymphocyte isolation, lysate preparations, SDS-PAGE, western blotting, dil-LDL uptake and ultracentrifugation were employed...
1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28239991/heterozygosity-of-the-721-221-b-51-01-cell-line-used-in-the-study-by-guasp-et-arthritis-rheumatol-february-2016
#2
(no author information available yet)
No abstract text is available yet for this article.
March 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28239986/molecular-analysis-of-the-early-interaction-between-the-grapevine-flower-and-botrytis-cinerea-reveals-that-prompt-activation-of-specific-host-pathways-leads-to-fungus-quiescence
#3
Zeraye H Mehari, Stefania Pilati, Paolo Sonego, Giulia Malacarne, Urska Vrhovsek, Kristof Engelen, Paul Tudzynski, Michela Zottini, Elena Baraldi, Claudio Moser
Grapes quality and yield can be impaired by bunch rot, caused by the necrotrophic fungus Botrytis cinerea. Infection often occurs at flowering and the pathogen stays quiescent until fruit maturity. Here, we report a molecular analysis of the early interaction between B. cinerea and Vitis vinifera flowers, using a controlled infection system, confocal microscopy and integrated transcriptomic and metabolic analysis of the host and the pathogen. Flowers from fruiting cuttings of the cv. Pinot Noir were infected with GFP-labeled B...
February 27, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28239912/verrucae-planae-within-previous-xenograft-sites-of-burn-wounds
#4
Olivia Chen, Michelle Vernali Pearlstein, Dean S Morrell, Sarah B Corley
Burn injuries are known to compromise host immune defenses through disruption of mucocutaneous barriers and suppression of cell-mediated immune responses, which may render patients with burn injuries susceptible to viral infections in the days to years after an initial insult. We report a case of verrucae planae developing as a secondary condition confined to former xenograft sites in a child, appearing more than 3.5 years after initial second-degree burn injuries. Only a few reports have previously described the development of verrucae in former burn sites, with most reporting latency to onset of verrucae appearance of months rather than years...
February 27, 2017: Pediatric Dermatology
https://www.readbyqxmd.com/read/28239901/molecular-prevalence-of-merkel-cell-polyomavirus-in-nonmelanoma-skin-cancer-in-a-brazilian-population
#5
T R Bellott, C F Baez, S G Almeida, M T Venceslau, M G Zalis, M A Guimarães, M C Rochael, F B Luz, R B Varella, J R Almeida
BACKGROUND: Merkel cell polyomavirus (MCPyV), a newly described oncogenic virus, has been found in association with tumours other than Merkel cell carcinoma (MCC). As yet, little is known about the involvement or influence of MCPyV on the development of these tumours and its prevalence in various populations. AIM: To assess the prevalence of MCPyV DNA in cases of nonmelanoma skin cancer (NMSC). METHODS: The prevalence of MCPyV DNA was assessed in 96 cases of NMSC in a Brazilian population comprising 76 subjects, and these results were correlated with epidemiological and demographical data...
February 27, 2017: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/28239897/population-pharmacokinetics-of-rituximab-in-patients-with-diffuse-large-b-cell-lymphoma-and-association-with-clinical-outcome
#6
S Rozman, I Grabnar, S Novaković, A Mrhar, B J Novaković
AIMS: Pharmacokinetic (PK) studies suggest that there is a room for improvement in clinical use of rituximab through more individualized treatment. The objective of this study was to characterize rituximab PK in 29 newly diagnosed patients with diffuse large B-cell lymphoma treated with rituximab in combination with cyclophosphamide, doxorubicin, vincristine and methylprednisolone every three weeks. We also evaluated the association of rituximab PK with clinical outcome. METHODS: Rituximab serum levels were determined by enzyme-linked immunosorbent assay and evaluated by a population PK analysis applying non-linear mixed effects modelling...
February 27, 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28239829/comparison-of-anterior-segment-parameter-values-obtained-with-scheimpflug-placido-topographer-optical-low-coherence-reflectometry-and-noncontact-specular-microscopy-in-morbid-obesity
#7
B Dogan, U Dogan, M K Erol, M Habibi, M T Oruc
OBJECTIVE: To investigate the measurement of anterior segment parameters using Sirius Scheimpflug-Placido topographer, Lenstar optical low coherence reflectometry (OLCR), and noncontact specular microscopy (SM) in morbidly obese and nonobese subjects. PATIENTS AND METHODS: Twenty-eight morbidly obese subjects (BMI ≥ 40; Group 1) and 28 age- sex-matched healthy nonobese subjects (BMI 18.50-24.99; Group 2) were included in this study. Anterior segment parameters were measured by Scheimpflug-Placido topographer and OLCR...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239825/upregulated-serum-sclerostin-level-in-the-t2dm-patients-with-femur-fracture-inhibits-the-expression-of-bone-formation-remodeling-associated-biomarkers-via-antagonizing-wnt-signaling
#8
Y Wu, S-Y Xu, S-Y Liu, L Xu, S-Y Deng, Y-B He, S-C Xian, Y-H Liu, G-X Ni
OBJECTIVE: Bone formation/remodeling-associated biomarkers, such as osteocalcin, amino pro-peptide of type 1 collagen (P1NP) and CrossLaps (CTX) have been deregulated in type 2 diabetes mellitus (T2DM) patients. In particular, the T2DM-associated sclerostin markedly inhibits the bone formation, suppresses the osteoblast activity and downregulates the bone turnover. PATIENTS AND METHODS: In the present study, we examined the serum levels of sclerostin, osteocalcin, P1NP and CTX in the T2DM patients...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239820/long-non-coding-rna-neat1-acts-as-oncogene-in-nsclc-by-regulating-the-wnt-signaling-pathway
#9
S-J Sun, Q Lin, J-X Ma, W-W Shi, B Yang, F Li
OBJECTIVE: The present study aimed to explore the role of long non-coding RNA NEAT1 (NEAT1) in mediating non-small cell lung cancer (NSCLC) cell migration and invasion, as well as the underlying regulatory mechanisms. PATIENTS AND METHODS: The NEAT1 expression in NSCLC tissues and cell lines was measured using reverse transcription‑quantitative polymerase chain reaction (RT-qPCR). The relationships between NEAT1 expression and clinicopathological parameters were examined by chi-square test...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239814/microrna15b-induced-smcc7721-apoptosis-via-down-regulation-of-xiap
#10
W-B Ding, Y-X Wang, C-W Dong
OBJECTIVE: microRNA can regulate cell growth, proliferation, and death; also, it can promote or inhibit cell growth and proliferation through regulation of apoptotic proteins. XIAP is a newly discovered pro-apoptotic protein, but how is XIAP regulated remains unclear. This study investigated the role of microRNA-15b in liver cancer cells' proliferation and apoptosis. MATERIALS AND METHODS: microRNA15b and control microRNA (miRNA) were purchased and transfected with hepatoma SMCC7721 cells using liposomal transfection techniques...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239754/neprilysin-facilitates-adipogenesis-through-potentiation-of-the-phosphatidylinositol-3-kinase-pi3k-signaling-pathway
#11
Juwan Kim, Dasol Han, Sung-Hyun Byun, Mookwang Kwon, Sun-Jung Cho, Young Ho Koh, Keejung Yoon
Neprilysin (NEP) is a zinc metallopeptidase that cleaves a number of small peptides into inactive forms. Despite the recent evidence of a significant correlation between the levels of NEP in plasma and the severity of obesity in humans, a cause-and-effect relationship or a functional role of NEP in obesity has remained uncertain. In this study, we show that NEP has a positive regulatory effect on fat cell formation from precursor cells. NEP increases the accumulation of cytoplasmic triglycerides in 3T3-L1 preadipocytes or the C3H10T1/2 mesenchymal stem cell line in differentiation conditions...
February 27, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28239687/protein-phosphatase-5-contributes-to-the-overexpression-of-epigenetically-regulated-t-lymphocyte-genes-in-patients-with-lupus
#12
D Patel, G Gorelik, B Richardson
OBJECTIVE: Lupus develops when genetically predisposed people encounter certain drugs or environmental agents causing oxidative stress such as infections and sun exposure, and then typically follows a chronic relapsing course with flares triggered by the exogenous stressors. Current evidence indicates that these environmental agents can trigger lupus flares by inhibiting the replication of DNA methylation patterns during mitosis in CD4(+) T cells, altering the expression of genes suppressed by this mechanism that convert normal "helper" cells into auto reactive cells which promote lupus flares...
December 2016: Lupus (Los Angel)
https://www.readbyqxmd.com/read/28239665/myeloid-12-15-lox-regulates-b-cell-numbers-and-innate-immune-antibody-levels-in-vivo
#13
Sarah N Lauder, Victoria J Tyrrell, Keith Allen-Redpath, Maceler Aldrovandi, David Gray, Peter Collins, Simon A Jones, Philip R Taylor, Valerie O'Donnell
Background. The myeloid enzyme 12/15-lipoxygenase (LOX), which generates bioactive oxidized lipids, has been implicated in numerous inflammatory diseases, with several studies demonstrating an improvement in pathology in mice lacking the enzyme. However, the ability of 12/15-LOX to directly regulate B cell function has not been studied. Methods. The influence of 12/15-LOX on B cell phenotype and function, and IgM generation, was compared using wildtype (WT) and 12/15-LOX (Alox15(-/-)) deficient mice. The proliferative and functional capacity of splenic CD19(+) B cells was measured in vitro in response to various toll-like receptor agonists...
January 4, 2017: Wellcome Open Res
https://www.readbyqxmd.com/read/28239658/loss-of-immune-homeostasis-dictates-shiv-rebound-after-stem-cell-transplantation
#14
Christopher W Peterson, Clarisse Benne, Patricia Polacino, Jasbir Kaur, Cristina E McAllister, Abdelali Filali-Mouhim, Willi Obenza, Tiffany A Pecor, Meei-Li Huang, Audrey Baldessari, Robert D Murnane, Ann E Woolfrey, Keith R Jerome, Shiu-Lok Hu, Nichole R Klatt, Stephen DeRosa, Rafick P Sékaly, Hans-Peter Kiem
The conditioning regimen used as part of the Berlin patient's hematopoietic cell transplant likely contributed to his eradication of HIV infection. We studied the impact of conditioning in simian-human immunodeficiency virus-infected (SHIV-infected) macaques suppressed by combination antiretroviral therapy (cART). The conditioning regimen resulted in a dramatic, but incomplete depletion of CD4(+) and CD8(+) T cells and CD20(+) B cells, increased T cell activation and exhaustion, and a significant loss of SHIV-specific Abs...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28239656/autoreactive-helper-t-cells-alleviate-the-need-for-intrinsic-tlr-signaling-in-autoreactive-b-cell-activation
#15
Josephine R Giles, Adriana Turqueti Neves, Ann Marshak-Rothstein, Mark J Shlomchik
T cells play a significant role in the pathogenesis of systemic autoimmune diseases, including systemic lupus erythematosus; however, there is relatively little information on the nature and specificity of autoreactive T cells. Identifying such cells has been technically difficult because they are likely to be rare and low affinity. Here, we report a method for identifying autoreactive T cell clones that recognize proteins contained in autoantibody immune complexes, providing direct evidence that functional autoreactive helper T cells exist in the periphery of normal mice...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28239644/hla-dq-%C3%AE-1-alleles-associated-with-epstein-barr-virus-ebv-infectivity-and-ebv-gp42-binding-to-cells
#16
Qingxue Li, Wei Bu, Erin Gabriel, Fiona Aguilar, Yo Hoshino, Hiroko Miyadera, Christoph Hess, Ronald L Hornung, Amitava Roy, Jeffrey I Cohen
Epstein-Barr virus (EBV) infects B cells and ~95% of adults are infected. EBV glycoprotein gp42 is essential for entry of virus into B cells. EBV gp42 binds to the β1 chain of HLA-DQ, -DR, and -DP on B cells, and uses these molecules for infection. To investigate if certain HLA-DQ alleles are associated with EBV seronegativity, we recruited ~3,300 healthy adult blood donors, identified 106 EBV-seronegative individuals, and randomly selected a control group of EBV-seropositive donors from the donor pool. A larger than expected proportion of EBV-seronegative subjects were HLA-DQ β1 *04/*05 and *06/*06, and to a lesser extent, *02/*03, compared with the control group, while a larger than expected portion of EBV-seropositive persons were HLA-DQ β1 *02/*02...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28239551/genome-wide-chromatin-accessibility-dna-methylation-and-gene-expression-analysis-of-histone-deacetylase-inhibition-in-triple-negative-breast-cancer
#17
Matias A Bustos, Matthew P Salomon, Nellie Nelson, Sandy C Hsu, Maggie L DiNome, Dave S B Hoon, Diego M Marzese
Triple-negative breast cancer (TNBC), especially the subset with a basal phenotype, represents the most aggressive subtype of breast cancer. Unlike other solid tumors, TNBCs harbor a low number of driver mutations. Conversely, we and others have demonstrated a significant impact of epigenetic alterations, including DNA methylation and histone post-translational modifications, affecting TNBCs. Due to the promising results in pre-clinical studies, histone deacetylase inhibitors (HDACi) are currently being tested in several clinical trials for breast cancer and other solid tumors...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28239394/standardization-and-validation-of-dot-elisa-assay-for-paracoccidioides-brasiliensis-antibody-detection
#18
Camila Mika Kamikawa, Rinaldo Poncio Mendes, Adriana Pardini Vicentini
BACKGROUND: Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28239381/presentation-of-autoantigen-in-peripheral-lymph-nodes-is-sufficient-for-priming-autoreactive-cd8-t-cells
#19
Nadine Honke, Namir Shaabani, John R Teijaro, Urs Christen, Cornelia Hardt, Judith Bezgovsek, Philipp A Lang, Karl S Lang
Peripheral tolerance is an important mechanism by which the immune system can guarantee a second line of defense against autoreactive T and B cells. One autoimmune disease that is related to a break of peripheral tolerance is diabetes mellitus type 1. Using the RIP-GP mouse model, we analyzed the role of the spleen and lymph nodes (LNs) in priming CD8(+) T cells and breaking peripheral tolerance. We found that diabetes developed in splenectomized mice infected with the lymphocytic choriomeningitis virus (LCMV), a finding showing that the spleen was not necessary in generating autoimmunity...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28239377/pro-inflammatory-signaling-in-a-3d-organotypic-skin-model-after-low-let-irradiation-nf-%C3%AE%C2%BAb-cox-2-activation-and-impact-on-cell-differentiation
#20
Anna Acheva, Giuseppe Schettino, Kevin M Prise
Nearly 85% of radiotherapy patients develop acute radiation dermatitis, which is an inflammatory reaction of the skin at the treatment field and in the surrounding area. The aims of this study were to unravel the mechanisms of radiation-induced inflammatory responses after localized irradiation in a human 3D organotypic skin culture model. This could provide possible inflammatory targets for reduction of skin side effects. 3D organotypic skin cultures were set up and locally irradiated with 225 kVp X-rays, using a combination of full exposure and partial shielding (50%) of the cultures...
2017: Frontiers in Immunology
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