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https://www.readbyqxmd.com/read/28110426/influenza-a-virus-upregulates-prpf8-gene-expression-to-increase-virus-production
#1
Chee-Hing Yang, Hui-Chun Li, Yu-Ling Shiu, Tzu-Shan Ku, Chia-Wen Wang, Yi-Shuan Tu, Hung-Ling Chen, Cheng-Hao Wu, Shih-Yen Lo
A ddRT-PCR analysis was performed to detect cellular genes that are differentially expressed after influenza A virus (H1N1) infection of A549 cells. After ddRT-PCR, eight DNA fragments were identified. PRPF8, one of the cellular genes that were upregulated after virus infection, was further analyzed since it has previously been identified as a cellular factor required for influenza virus replication. The upregulation of PRPF8 gene expression after viral infection was confirmed using real-time RT-PCR for mRNA detection and Western blot analysis for protein detection...
January 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28110416/lasiokaurin-derivatives-synthesis-antimicrobial-and-antitumor-biological-evaluation-and-apoptosis-inducing-effects
#2
Da-Hong Li, Ping Hu, Sheng-Tao Xu, Chun-Yan Fang, Shuang Tang, Xin-Yu Wang, Xing-Yan Sun, He Li, Ying Xu, Xiao-Ke Gu, Jin-Yi Xu
Herein, a series of lasiokaurin derivatives were designed and synthesized. All the derivatives together with lasiokaurin and oridonin were tested for their antimicrobial and antiproliferative activity. Compound 16 showed the most promising antimicrobial activity with MICs of 2.0 and 1.0 μg/mL against Gram-Positive bacteria S. aureus and B. subtilis, respectively. All the synthetic lasiokaurin derivatives showed better antiproliferative activity than parent compound lasiokaurin 1. Compound 10 exhibited the strongest cytotoxicity with IC50 values of 0...
January 21, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28110394/chimeric-antigen-receptor-car-t-cells-lessons-learned-from-targeting-of-cd19-in-b-cell-malignancies
#3
Kevin A Hay, Cameron J Turtle
Adoptive immunotherapy with chimeric antigen receptor-modified (CAR)-T cells is a rapidly growing therapeutic approach to treating patients with refractory cancer, with over 100 clinical trials in various malignancies in progress. The enthusiasm for CAR-T cells has been driven by the clinical success of CD19-targeted CAR-T cell therapy in B-cell acute lymphoblastic leukemia, and the promising data in B-cell non-Hodgkin's lymphoma and chronic lymphocytic leukemia. Despite the success of targeting CD19 with CAR-T cells in early clinical studies, many challenges remain to improve outcomes, reduce toxicity, and determine the appropriate settings for CAR-T cell immunotherapy...
January 21, 2017: Drugs
https://www.readbyqxmd.com/read/28110347/68ga-pentixafor-pet-ct-demonstrating-higher-cxcr4-density-in-small-cell-lung-carcinoma-than-in-non-small-cell-variant
#4
Baljinder Singh, Ankit Watts, Rajender Basher, Harmandeep Singh, Amanjit Bal, Rakesh Kapoor, Sunil K Arora, Hans J Wester, B R Mittal, D Behera
No abstract text is available yet for this article.
January 21, 2017: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28110254/leptomeningeal-carcinomatosis-in-non-small-cell-lung-cancer-patients-a-continuing-challenge-in-the-personalized-treatment-era
#5
REVIEW
J Remon, E Le Rhun, B Besse
Leptomeningeal metastasis is a fatal manifestation seen in advanced cancer patients. Its incidence is increasing, reaching 3.8% in molecularly unselected non-small cell lung cancer patients and up to 5% and 9% in ALK-rearranged and EGFR-mutant lung cancer patients, respectively. The prognosis remains poor despite systemic treatment, intrathecal chemotherapy, radiation therapy and personalized treatments in molecularly selected patients. However, new therapies with improved cerebral-spinal fluid penetration have been developed for subgroups of molecular selected patients indicating they could be promising therapeutic options for managing leptomeningeal disease...
December 30, 2016: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28110215/neuroprotective-action-of-n-acetyl-serotonin-in-oxidative-stress-induced-apoptosis-through-the-activation-of-both-trkb-creb-bdnf-pathway-and-akt-nrf2-antioxidant-enzyme-in-neuronal-cells
#6
Jae-Myung Yoo, Bo Dam Lee, Dai-Eun Sok, Jin Yuel Ma, Mee Ree Kim
N-acetyl serotonin (NAS) as a melatonin precursor has neuroprotective actions. Nonetheless, it is not clarified how NAS protects neuronal cells against oxidative stress. Recently, we have reported that N-palmitoyl serotonins possessed properties of antioxidants and neuroprotection. Based on those, we hypothesized that NAS, a N-acyl serotonin, may have similar actions in oxidative stress-induced neuronal cells, and examined the effects of NAS based on in vitro and in vivo tests. NAS dose-dependently inhibited oxidative stress-induced cell death in HT-22 cells...
January 6, 2017: Redox Biology
https://www.readbyqxmd.com/read/28110195/geometric-protein-localization-cues-in-bacterial-cells
#7
REVIEW
Taylor B Updegrove, Kumaran S Ramamurthi
Bacterial cells are highly organized at a molecular level. Understanding how specific proteins localize to their proper subcellular address has been a major challenge in bacterial cell biology. One mechanism, which appears to be increasingly more common, is the use of 'geometric cues' for protein localization. In this model, certain shape-sensing proteins recognize, and preferentially embed into, either negatively or positively curved (concave or convex, respectively) membranes. Here, we review examples of bacterial proteins that reportedly localize by sensing geometric cues and highlight emerging mechanistic understandings of how proteins may recognize subtle differences in membrane curvature...
January 19, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28110098/clinical-manifestations-of-helicobacter-pylori-negative-gastritis
#8
Seiji Shiota, Aaron P Thrift, Linda Green, Rajesh Shah, Gordana Verstovsek, Massimo Rugge, David Y Graham, Hashem B El-Serag
OBJECTIVES: There are data to suggest the existence of non-H. pylori gastritis. However, the risk factors and clinical course for H. pylori-negative gastritis remain unclear. We aimed to examine the prevalence and determinants of H. pylori-negative gastritis in a large multiethnic clinical population. METHOD: We conducted a cross-sectional study among patents scheduled for an elective esophagastroduodenoscopy or attending selected primary care clinics and eligible for screening colonoscopy at a single VA medical center...
January 18, 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28110075/tlr9-mediated-regulatory-b10-cell-amplification-following-sub-total-body-irradiation-implications-in-attenuating-eae
#9
Jinsheng Hong, Jie Fang, Ruilong Lan, Qi Tan, Yeping Tian, Mei Zhang, Paul Okunieff, Lurong Zhang, Jianhua Lin, Deping Han
Autoimmunity and inflammation are controlled in part by regulatory B (Breg) cells, including the recently identified IL-10-competent B10 cell subset that represents 1%-3% of mouse spleen B cells. In this study, the influence of irradiation on Breg/B10 cell generation and IL-10 production mediated by TLR9 signaling pathways was investigated. Spleen and peritoneal cavity Breg/B10 cell frequencies were significantly expanded three weeks after sub-total body irradiation (sub-TBI, 5Gy or 10Gy) in adult male wild type (WT) C57BL/6(B6) mice but not in TLR9(-/-) mice...
January 18, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28110039/heat-induced-solubilization-of-curcumin-in-kinetically-stable-pluronic-p123-micelles-and-vesicles-an-exploit-of-slow-dynamics-of-the-micellar-restructuring-processes-in-the-aqueous-pluronic-system
#10
R Ganguly, A Kunwar, B Dutta, S Kumar, K C Barick, A Ballal, V K Aswal, P A Hassan
Wide therapeutic potential combined with low cost and negligible toxicity makes curcumin one of the most sought after drugs in recent times. Its poor aqueous solubility and low bioavailability are often overcome by using micelles and vesicles as its carriers. The substances that are commonly used for this purpose are a class of nonionic surfactants called pluronics. Solubilization of curcumin in aqueous systems of these surfactants is carried out by thin film hydration method presumably because slow dynamics of micellar restructuring processes in them creates hindrance for direct solubilization...
January 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28109985/the-synthetic-neuroactive-steroid-sge-516-reduces-status-epilepticus-and-neuronal-cell-death-in-a-rat-model-of-soman-intoxication
#11
Alison L Althaus, Hilary S McCarren, Aymen Alqazzaz, Cecelia Jackson, John H McDonough, Carl D Smith, Ethan Hoffman, Rebecca S Hammond, Albert J Robichaud, James J Doherty
Organophosphorus nerve agents (OPNAs) are irreversible inhibitors of acetylcholinesterase that pose a serious threat to public health because of their use as chemical weapons. Exposure to high doses of OPNAs can dramatically potentiate cholinergic synaptic activity and cause status epilepticus (SE). Current standard of care for OPNA exposure involves treatment with cholinergic antagonists, oxime cholinesterase reactivators, and benzodiazepines. However, data from pre-clinical models suggest that OPNA-induced SE rapidly becomes refractory to benzodiazepines...
January 18, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28109957/adoptive-transfer-of-mrna-transfected-t-cells-redirected-against-diabetogenic-cd8%C3%A2-t-cells-can-prevent-diabetes
#12
Sigal Fishman, Mark D Lewis, L Khai Siew, Evy De Leenheer, Dimitri Kakabadse, Joanne Davies, Doron Ziv, Alon Margalit, Nathan Karin, Gideon Gross, F Susan Wong
Chimeric major histocompatibility complex (MHC) molecules supplemented with T cell receptor (TCR) signaling motifs function as activation receptors and can redirect gene-modified T cells against pathogenic CD8 T cells. We have shown that β2 microglobulin (β2m) operates as a universal signaling component of MHC-I molecules when fused with the CD3-ζ chain. Linking the H-2K(d)-binding insulin B chain peptide insulin B chain, amino acids 15-23 (InsB15-23) to the N terminus of β2m/CD3-ζ, redirected polyclonal CD8 T cells against pathogenic CD8 T cells in a peptide-specific manner in the non-obese diabetic (NOD) mouse...
January 18, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28109815/a-novel-biocompatible-drug-delivery-system-of-chitosan-temozolomide-nanoparticles-loaded-pcl-pu-nanofibers-for-sustained-delivery-of-temozolomide
#13
Mohammad Irani, Gity Mir Mohamad Sadeghi, Ismaeil Haririan
In the present study, the anticancer drug temozolomide (TMZ) was initially loaded into the chitosan (CS) nanoparticles and synthesized CS/TMZ nanoparticles were incorporated into the synthesized poly (ε-caprolactone diol) based polyurethane (PCL-Diol-b-PU) nanofibers. The synthesized nanoparticles and nanofibers were characterized using dynamic light scattering, transmission electron microscopy, X-ray powder diffraction and scanning electron microscopy. The obtained results revealed that the CS/TMZ nanoparticles were successfully embedded into the PCL-Diol-b-PU nanofibers...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109813/amyloid-like-aggregates-formation-by-blood-plasma-fibronectin
#14
Rümeyza Bascetin, Khadija Admane, Rémy Agniel, Thomas Boudou, Tristan Doussineau, Rodolphe Antoine, Olivier Gallet, Johanne Leroy-Dudal, Charlotte Vendrely
Fibronectin (FN) is a multifunctional glycoprotein of the extracellular matrix (ECM) playing critical roles in physiological and pathological cell processes like adhesion, migration, growth, and differentiation. These various functions of FN are modulated by its supramolecular state. Indeed, FN can polymerize into different types of assemblies like fibrils and aggregates. However, the mechanism of polymerization and the effects of such assemblies on cell behaviors still remain to be elucidated. Here we show that upon irreversible thermal denaturation, human blood plasma fibronectin forms high molecular weight aggregates...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109806/momordica-charantia-polysaccharides-ameliorate-oxidative-stress-hyperlipidemia-inflammation-and-apoptosis-during-myocardial-infarction-by-inhibiting-the-nf-%C3%AE%C2%BAb-signaling-pathway
#15
Mohammad Raish
The polysaccharide extract of Momordica charantia has various biological activities; however, its effect on endothelial dysfunction in myocardial infarction remains unclear. To elucidate this, myocardial infarction was induced in rats using isoproterenol (ISP). Pretreatment with M. charantia polysaccharides (MCP; 150 or 300mg/kg) for 25days significantly inhibited increases in heart weight, the heart-weight-to-body-weight ratio, and infarction size, and ameliorated the increased serum levels of aspartate transaminase, creatine kinase, lactate dehydrogenase, total cholesterol, triglycerides, very-low-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109707/potential-risk-of-repeated-nasal-vaccination-that-induces-allergic-reaction-with-mucosal-ige-and-airway-eosinophilic-infiltration-in-cynomolgus-macaques-infected-with-h5n1-highly-pathogenic-avian-influenza-virus
#16
Misako Nakayama, Yasushi Itoh, Shintaro Shichinohe, Rumi Nakabayashi, Hirohito Ishigaki, Yoshihiro Sakoda, Quynh Mai Le, Yoshihiro Kawaoka, Hiroshi Kida, Kazumasa Ogasawara
The efficacy and detrimental effect of mucosal vaccination with an inactivated influenza vaccine were examined in a macaque model by intranasal administration with small amounts of inactivated whole virus particles and challenge by a human-derived H5N1 highly pathogenic avian influenza virus infection. Repeated nasal inoculation with the whole particle vaccine of an inactivated virus, A/duck/Hokkaido/Vac-3/2007 (H5N1) (Vac-3), induced antigen-specific IgA and IgG antibody production in nasal swabs and plasma...
January 18, 2017: Vaccine
https://www.readbyqxmd.com/read/28109599/effect-of-intramammary-infusion-of-chitosan-hydrogels-at-drying-off-on-bovine-mammary-gland-involution
#17
S Lanctôt, P Fustier, A R Taherian, B Bisakowski, X Zhao, P Lacasse
The transition from lactation to the dry period in dairy cows is a period of high risk for acquiring new intramammary infections. This risk is reduced when the involution of the mammary gland is completed. Accordingly, approaches that speed up the involution process after drying-off could reduce the incidence of mastitis. The current study aimed to develop a biological response modifier that could be injected into cow teats to promote immune cell migration and speed up involution. Chitosan, a natural polysaccharide derived from chitin, is able to trigger host innate immunity...
January 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28109572/subcarinal-lymph-nodes-should-be-dissected-in-all-lobectomies-for-non-small-cell-lung-cancer-regardless-of-primary-tumor-location
#18
Jens Eckardt, Erik Jakobsen, Peter B Licht
BACKGROUND: Mediastinal staging is of paramount importance for planning of treatment in non-small cell lung cancer (NSCLC). Single institution reports recently claimed that subcarinal lymph node dissection during resection of upper lobe NSCLC could be spared. We used a complete national lung cancer registry to investigate patterns of unsuspected mediastinal lymph node involvement after lobectomy. METHODS: During an 11-year period (2004 to 2014) 5,577 consecutive patients who underwent operations for NSCLC were investigated for unsuspected mediastinal lymph node involvement (N2 disease) discovered at final histopathology...
January 18, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28109439/deep-profiling-of-proteome-and-phosphoproteome-by-isobaric-labeling-extensive-liquid-chromatography-and-mass-spectrometry
#19
B Bai, H Tan, V R Pagala, A A High, V P Ichhaporia, L Hendershot, J Peng
Mass spectrometry-based proteomics has experienced an unprecedented advance in comprehensive analysis of proteins and posttranslational modifications, with particular technical progress in liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and isobaric labeling multiplexing capacity. Here, we introduce a deep proteomics profiling protocol that combines 10-plex tandem mass tag (TMT) labeling with an optimized LC-MS/MS platform to quantitate whole proteome and phosphoproteome. The major steps include protein extraction and digestion, TMT labeling, two-dimensional liquid chromatography, TiO2-mediated phosphopeptide enrichment, high-resolution mass spectrometry, and computational data processing...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109371/-second-primary-cardiac-sarcoma-in-a-patient-with-ewing-sarcoma-always-expect%C3%A2-the-unexpected
#20
Antonino Di Franco, Mario Gaudino, Jonathan W Weinsaft, Shawn C Pun, Navneet Narula, Saadat A Khan, Zahra M Malik, Lucas B Ohmes, Nickolaos J Skubas, Leonard N Girardi
A high suspicion for relapsed metastatic disease must arise when an intracardiac mass is detected in a patient with a recent history of Ewing sarcoma. Nevertheless, the scenario may eventually turn out to be much more complex than expected, and the possibility that the intracardiac tumor may instead be a "second" primary sarcoma, although extremely rare, should also be considered. We describe the first case of concomitant diagnosis of Ewing sarcoma and low-grade myxoid spindle cell sarcoma in the same young patient...
February 2017: Annals of Thoracic Surgery
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