keyword
MENU ▼
Read by QxMD icon Read
search

Multidrug resistant gram negative bacteria

keyword
https://www.readbyqxmd.com/read/29147114/antibiotic-resistant-bacterial-isolates-from-captive-green-turtles-and-in-vitro-sensitivity-to-bacteriophages
#1
Alessandro Delli Paoli Carini, Ellen Ariel, Jacqueline Picard, Lisa Elliott
This study aimed to test multidrug resistant isolates from hospitalised green turtles (Chelonia mydas) and their environment in North Queensland, Australia, for in vitro susceptibility to bacteriophages. Seventy-one Gram-negative bacteria were isolated from green turtle eye swabs and water samples. Broth microdilution tests were used to determine antibiotic susceptibility. All isolates were resistant to at least two antibiotics, with 24% being resistant to seven of the eight antibiotics. Highest resistance rates were detected to enrofloxacin (77%) and ampicillin (69...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29138303/cxc-chemokines-exhibit-bactericidal-activity-against-multidrug-resistant-gram-negative-pathogens
#2
Matthew A Crawford, Debra J Fisher, Lisa M Leung, Sara Lomonaco, Christine Lascols, Antonio Cannatelli, Tommaso Giani, Gian Maria Rossolini, Yohei Doi, David R Goodlett, Marc W Allard, Shashi K Sharma, Erum Khan, Robert K Ernst, Molly A Hughes
The continued rise and spread of antimicrobial resistance among bacterial pathogens pose a serious challenge to global health. Countering antimicrobial-resistant pathogens requires a multifaceted effort that includes the discovery of novel therapeutic approaches. Here, we establish the capacity of the human CXC chemokines CXCL9 and CXCL10 to kill multidrug-resistant Gram-negative bacteria, including New Delhi metallo-beta-lactamase-1-producing Klebsiella pneumoniae and colistin-resistant members of the family Enterobacteriaceae that harbor the mobile colistin resistance protein MCR-1 and thus possess phosphoethanolamine-modified lipid A...
November 14, 2017: MBio
https://www.readbyqxmd.com/read/29128373/regulation-of-the-acrab-tolc-efflux-pump-in-enterobacteriaceae
#3
Natasha Weston, Prateek Sharma, Vito Ricci, Laura J V Piddock
Bacterial multidrug efflux systems are a major mechanism of antimicrobial resistance and are fundamental to the physiology of Gram-negative bacteria. The resistance-nodulation-division (RND) family of efflux pumps is the most clinically significant, as it is associated with multidrug resistance. Expression of efflux systems is subject to multiple levels of regulation, involving local and global transcriptional regulation as well as post-transcriptional and post-translational regulation. The best-characterised RND system is AcrAB-TolC, which is present in Enterobacteriaceae...
November 8, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/29116010/multi-drug-resistance-and-extended-spectrum-beta-lactamase-producing-gram-negative-bacteria-from-chicken-meat-in-bharatpur-metropolitan-nepal
#4
Anil Shrestha, Anup Muni Bajracharya, Hemraj Subedi, Raju Shah Turha, Sachin Kafle, Saroj Sharma, Sunil Neupane, Dhiraj Kumar Chaudhary
OBJECTIVE: Multidrug resistance (MDR) and extended spectrum beta lactamase (ESBL) producer Gram negative bacteria are considered as a major health problem, globally. ESBL enzyme hydrolyses the beta lactam ring of third generation cephalosporins, which alters the structure of the antibiotic. Due to the modification in structure of the antibiotic, bacteria show resistance to these antibiotics. Resistant bacterial strains are transmitted to humans from animals through consumption of uncooked meat, through contact with uncooked meat and meat surfaces...
November 7, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29111434/empiric-treatment-of-lower-urinary-tract-infections-in-the-face-of-spreading-multi-drug-resistance-in-vitro-study-of-nitroxoline-effectiveness
#5
A Sobke, O Makarewicz, M Baier, C Bär, W Pfister, S G Gatermann, M W Pletz, C Forstner
The spread of antimicrobial resistance challenges the empiric treatment in urinary tract infections (UTI). Among others, nitrofurantoin is recommended for first line treatment, but acceptance among clinicians is limited due to chronic nitrofurantoin-induced lung toxicity and insufficient coverage of Enterobacteriaceae other than Escherichia coli. Nitroxoline seems to be an alternative to nitrofurantoin due to its favourable safety profile but data on its current in vitro susceptibility are sparse. In this study susceptibility to nitroxoline was tested against 3,012 urinary clinical isolates (including multidrug-resistant bacteria and Candida spp...
October 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29108951/automated-time-lapse-microscopy-a-novel-method-for-screening-of-antibiotic-combination-effects-against-multidrug-resistant-gram-negative-bacteria
#6
Wanchana Ungphakorn, Pernilla Lagerbäck, Elisabet I Nielsen, Thomas Tängdén
OBJECTIVES: Antibiotic combinations are often used for carbapenemase-producing Enterobacteriaceae (CPE) but more data are needed on the optimal selection of drugs. This study aimed to evaluate the feasibility of a novel automated method based on time-lapse microscopy (the oCelloScope, Philips BioCell A/S, Allerød, Denmark) to determine in vitro combination effects against CPE and to discuss advantages and limitations of the oCelloScope in relation to standard methods. METHODS: Four Klebsiella pneumoniae and two Escherichia coli were exposed to colistin, meropenem, rifampin and tigecycline, alone and in combination...
November 3, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29104124/control-of-endemic-multidrug-resistant-gram-negative-bacteria-after-removal-of-sinks-and-implementing-a-new-water-safe-policy-in-an-intensive-care-unit
#7
Evelyn Shaw, Laura Gavaldà, Jordi Càmara, Rosa Gasull, Santiago Gallego, Fe Tubau, Rosa Maria Granada, Pilar Ciercoles, M Angeles Dominguez, Rafael Mañez, Jordi Carratalà, Miquel Pujol
BACKGROUND: Contaminated handwashing sinks have been identified as reservoirs that can facilitate colonisation/infection of patients with multidrug-resistant (MDR) gram-negative bacteria (GNB) in intensive care units (ICUs). To eliminate endemic MDR-GNB in our ICU, we implemented a water-safe policy that involved the removal of all sinks from patients' rooms. AIM: We aimed to assess the impact of removing patients' sinks and implementing other water-safe strategies on the annual rates of ICU-acquired MDR-GNB...
November 2, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29104098/stigmasterol-an-adjuvant-for-beta-lactam-antibiotics-against-beta-lactamase-positive-clinical-isolates
#8
Tong Woei Yenn, Muhammad Arslan Khan, Nur Amiera Syuhada, Leong Chean Ring, Darah Ibrahim, Wen-Nee Tan
The emergence of beta lactamase producing bacterial strains eliminated the use of beta lactam antibiotics as chemotherapeutic alternative. Beta lactam antibiotics can be coupled with non-antibiotic adjuvants to combat these multidrug resistant strains. We study the synergistic antibiotic effect of stigmasterol as adjuvant of ampicillin against clinical isolates. Ampicillin was used in this study as a beta lactam antibiotic model. All test bacteria were beta lactamase producing clinical isolates. The combination showed significantly better antibiotic activity on all bacteria tested...
November 7, 2017: Steroids
https://www.readbyqxmd.com/read/29102775/blandm-1-producing-multidrug-resistant-escherichia-coli-isolated-from-a-companion-dog-in-china
#9
Lanqing Cui, Lei Lei, Yuan Lv, Rongmin Zhang, Xiaoyu Liu, Mei Li, Fan Zhang, Yang Wang
OBJECTIVES: This sudy aimed to characterize a blaNDM-1-producing Escherichia coli from a companion dog. METHODS: This strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria and the specie was identified by API 20E system and MALDI-TOF MS. MICs of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) and various carbapenemase genes including blaNDM, blaIMP, blaVIM, blaSME, blaIMI, blaKPC, blaGES, blaSPM, blaGIM, blaNMC, blaDIM, blaSIM, blaOXA-23, blaOXA-24, blaOXA-48, blaOXA-51 and blaOXA-58 were screened by PCR...
November 1, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29089624/combating-virulence-of-gram-negative-bacilli-by-ompa-inhibition
#10
Xavier Vila-Farrés, Raquel Parra-Millán, Viviana Sánchez-Encinales, Monica Varese, Rafael Ayerbe-Algaba, Nuria Bayó, Salvador Guardiola, María Eugenia Pachón-Ibáñez, Martin Kotev, Jesús García, Meritxell Teixidó, Jordi Vila, Jerónimo Pachón, Ernest Giralt, Younes Smani
Preventing the adhesion of pathogens to host cells provides an innovative approach to tackling multidrug-resistant bacteria. In this regard, the identification of outer membrane protein A (OmpA) as a key bacterial virulence factor has been a major breakthrough. The use of virtual screening helped us to identify a cyclic hexapeptide AOA-2 that inhibits the adhesion of Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli to host cells and the formation of biofilm, thereby preventing the development of infection in vitro and in a murine sepsis peritoneal model...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29089611/molecular-insights-into-antimicrobial-resistance-traits-of-multidrug-resistant-enteric-pathogens-isolated-from-india
#11
Pawan Kumar, Satyabrata Bag, Tarini Shankar Ghosh, Prasanta Dey, Mayanka Dayal, Bipasa Saha, Jyoti Verma, Archana Pant, Shruti Saxena, Anbumani Desigamani, Preety Rana, Dhirendra Kumar, Naresh C Sharma, Pranita Hanpude, Tushar K Maiti, Asish K Mukhopadhyay, Rupak K Bhadra, G Balakrish Nair, Thandavarayan Ramamurthy, Bhabatosh Das
Emergence of antimicrobial resistant Gram-negative bacteria has created a serious global health crisis and threatens the effectiveness of most, if not all, antibiotics commonly used to prevent and treat bacterial infections. There is a dearth of detailed studies on the prevalence of antimicrobial resistance (AMR) patterns in India. Here, we have isolated and examined AMR patterns of 654 enteric pathogens and investigated complete genome sequences of isolates from six representative genera, which in aggregate encode resistance against 22 antibiotics representing nine distinct drug classes...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29089523/combined-application-of-bacterial-predation-and-violacein-to-kill-polymicrobial-pathogenic-communities
#12
Hansol Im, Seong Yeol Choi, Sangmo Son, Robert J Mitchell
Violacein is a bisindole antibiotic that is effective against Gram-positive bacteria while the bacterial predator, Bdellovibrio bacteriovorus HD100, predates on Gram-negative strains. In this study, we evaluated the use of both together against multidrug resistant pathogens. The two antibacterial agents did not antagonize the activity of the other. For example, treatment of Staphylococcus aureus with violacein reduced its viability by more than 2,000-fold with or without B. bacteriovorus addition. Likewise, predation of Acinetobacter baumannii reduced the viability of this pathogen by more than 13,000-fold, regardless if violacein was present or not...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29089426/synergy-between-active-efflux-and-outer-membrane-diffusion-defines-rules-of-antibiotic-permeation-into-gram-negative-bacteria
#13
Ganesh Krishnamoorthy, Inga V Leus, Jon W Weeks, David Wolloscheck, Valentin V Rybenkov, Helen I Zgurskaya
Gram-negative bacteria are notoriously resistant to antibiotics, but the extent of the resistance varies broadly between species. We report that in significant human pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Burkholderia spp., the differences in antibiotic resistance are largely defined by their penetration into the cell. For all tested antibiotics, the intracellular penetration was determined by a synergistic relationship between active efflux and the permeability barrier. We found that the outer membrane (OM) and efflux pumps select compounds on the basis of distinct properties and together universally protect bacteria from structurally diverse antibiotics...
October 31, 2017: MBio
https://www.readbyqxmd.com/read/29079617/staphylococcus-edaphicus-sp-nov-isolated-in-antarctica-harbours-mecc-gene-and-genomic-islands-with-suspected-role-in-adaptation-to-extreme-environment
#14
Roman Pantůček, Ivo Sedláček, Adéla Indráková, Veronika Vrbovská, Ivana Mašlaňová, Vojtěch Kovařovic, Pavel Švec, Stanislava Králová, Lucie Krištofová, Jana Kekláková, Petr Petráš, Jiří Doškař
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus Staphylococcus that has near identical 16S rRNA gene sequence to Staphylococcus saprophyticus However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity <85 %, and inferred DNA-DNA hybridization <30%...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29073359/complete-genome-sequence-of-enterobacter-cloacae-r11-reveals-multiple-genes-potentially-associated-with-high-level-polymyxin-e-resistance
#15
Chuanqing Zhong, Chao Zhang, Jiafang Fu, Wenbing Chen, Tianyi Jiang, Guangxiang Cao
Enterobacter cloacae strain R11 is a multidrug-resistant bacterium isolated from sewage water near a swine feedlot in China. Strain R11 can survive in medium with up to 192 μg/ml polymyxin E, indicating a tolerance for this antibiotic significantly higher than that reported for other gram-negative bacteria. In this study, conjugation experiments showed that partial polymyxin E resistance could be transferred from strain R11 to Escherichia coli strain 25922, revealing that some genes related to polymyxin E resistance are plasmid-based...
October 26, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29067005/characterization-of-four-multidrug-resistance-plasmids-captured-from-the-sediments-of-an-urban-coastal-wetland
#16
Ryan T Botts, Brooke A Apffel, C J Walters, Kelly E Davidson, Ryan S Echols, Michael R Geiger, Victoria L Guzman, Victoria S Haase, Michal A Montana, Chip A La Chat, Jenna A Mielke, Kelly L Mullen, Cierra C Virtue, Celeste J Brown, Eva M Top, David E Cummings
Self-transmissible and mobilizable plasmids contribute to the emergence and spread of multidrug-resistant bacteria by enabling the horizontal transfer of acquired antibiotic resistance. The objective of this study was to capture and characterize self-transmissible and mobilizable resistance plasmids from a coastal wetland impacted by urban stormwater runoff and human wastewater during the rainy season. Four plasmids were captured, two self-transmissible and two mobilizable, using both mating and enrichment approaches...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29052670/novel-bis-cyclic-guanidines-as-potent-membrane-active-antibacterial-agents-with-therapeutic-potential
#17
Peng Teng, Alekhya Nimmagadda, Ma Su, Yuzhu Hong, Ning Shen, Chunpu Li, Ling-Yu Tsai, Jessica Cao, Qi Li, Jianfeng Cai
We designed a class of small dimeric cyclic guanidine derivatives which display potent antibacterial activity against both multidrug-resistant Gram-negative and Gram-positive bacteria. They could compromise bacterial membranes without developing resistance, inhibit biofilms formed by E. coli, and exhibit excellent in vivo activity in the MRSA-infected thigh burden mouse model.
October 31, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29046668/characterization-of-anti-bacterial-compounds-from-the-seed-coat-of-chinese-windmill-palm-tree-trachycarpus-fortunei
#18
Shakeel Ahmed, Huimin Liu, Aqeel Ahmad, Waheed Akram, Eman K N Abdelrahman, Fengming Ran, Wuling Ou, Shuang Dong, Qian Cai, Qiyun Zhang, Xiaohua Li, Sheng Hu, Xuebo Hu
The increasing of multidrug resistance in bacterial associated infections has impaired the current antimicrobial therapy and it forces the search for other alternatives. In this study, we aimed to find the in vitro antibacterial activity of seed coat of Trachycarpus fortunei against a panel of clinically important bacterial species. Ethanolic extracts of target tissues were fractionated through macro porous resin by column chromatography, using ethanol as an organic solvent with a concentration gradient of 0-100%, each along with 20% concentration increment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29042652/computational-analysis-of-the-molecular-mechanism-of-ramr-mutations-contributing-to-antimicrobial-resistance-in-salmonella-enterica
#19
Yen-Yi Liu, Chih-Chieh Chen
Antimicrobial resistance (AMR) in pathogenic microorganisms with multidrug resistance (MDR) constitutes a severe threat to human health. A major causative mechanism of AMR is mediated through the multidrug efflux pump (MEP). The resistance-nodulation-division superfamily (RND family) of Gram-negative bacteria is usually the major cause of MDR in clinical studies. In Salmonella enterica, the RND pump is translated from the arcAB gene, which is regulated by the activator RamA. Many MEP-caused AMR strains have high ramA gene expression due to mutations in RamR, which has a homodimeric structure comprising the dimerization domain and DNA-binding domain (DBD)...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29039717/high-prevalence-of-multidrug-resistant-bacteria-in-libyan-war-casualties-admitted-to-a-tertiary-care-hospital-germany
#20
Benedikt Lohr, Yvonne Pfeifer, Ursel Heudorf, Christoph Rangger, Douglas E Norris, Klaus-Peter Hunfeld
The ongoing Libyan conflict constantly causes victims among the military and civilian population. Cross-border transfer of patients represents a high risk of introducing multidrug-resistant organisms (MDROs), for example, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci, and carbapenem-resistant gram-negative organisms (CROs), into the country of destination. This study assessed the MDRO status in Libyan war casualties (n = 67) admitted to Northwest Medical Centre in Frankfurt/Main, Germany, from August 2016 till January 2017...
October 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
keyword
keyword
42576
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"