keyword
MENU ▼
Read by QxMD icon Read
search

Alcohol and enzyme

keyword
https://www.readbyqxmd.com/read/28220703/whole-cell-biocatalytic-synthesis-of-cinnamyl-acetate-with-a-novel-esterase-from-the-dna-library-of-acinetobacter-haemolyticus
#1
Hao Dong, Francesco Secundo, Changhu Xue, Xiangzhao Mao
Cinnamyl acetate has a wide application in the flavor and fragrance industry because of its sweet, balsamic and floral odor. Up to now, lipases have been mainly used in enzyme-mediated synthesis of cinnamyl acetate, while esterases are only used in a few cases. Moreover, the use of purified enzymes is often a disadvantage which leads to increases of the production costs. In this paper, a genomic DNA library of Acinetobacter haemolyticus was constructed, and a novel esterase (EstK1) was identified. After expressed in E...
February 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28220621/a-low-prevalence-of-non-alcoholic-fatty-liver-disease-in-patients-with-type-1-diabetes-is-associated-to-decreased-subclinical-cardiovascular-disease
#2
Enric Serra-Planas, Eva Aguilera, Laura Castro, Raúl Rodríguez, Isabel Salinas, Anna Lucas, Clara Joaquín, Rocio Puig, Dídac Mauricio, Manel Puig-Domingo
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) has been proposed as an independent cardiovascular risk factor. This study evaluated the prevalence of NAFLD in a cohort of Type 1 Diabetic (T1D) patients and its potential relationship with subclinical cardiovascular disease (CVD). METHODS: Cross-sectional study in which 100 T1D patients (39.4 ± 7.8 years, 54% male, all Caucasian, disease duration 21.7 ± 8.6 years, HbA1c 8.1 ± 1.0 % (64.2 ± 7...
February 20, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/28218773/risk-factors-for-birth-defects
#3
Benjamin S Harris, Katherine C Bishop, Hanna R Kemeny, Jennifer S Walker, Eleanor Rhee, Jeffrey A Kuller
Importance: Major congenital abnormalities, or birth defects, carry significant medical, surgical, cosmetic, or lifestyle consequences. Such abnormalities may be syndromic, involving multiple organ systems, or can be isolated. Overall, 2% to 4% of live births involve congenital abnormalities. Risk factors for birth defects are categorized as modifiable and nonmodifiable. Modifiable risk factors require thorough patient education/counseling. The strongest risk factors, such as age, family history, and a previously affected child, are usually nonmodifiable...
February 2017: Obstetrical & Gynecological Survey
https://www.readbyqxmd.com/read/28218651/proteome-characteristics-of-non-alcoholic-steatohepatitis-liver-tissue-and-associated-hepatocellular-carcinomas
#4
Anna Kakehashi, Vasily E Stefanov, Naomi Ishii, Takahiro Okuno, Hideki Fujii, Kazuaki Kawai, Norifumi Kawada, Hideki Wanibuchi
To uncover mechanisms of nonalcoholic steatohepatitis (NASH) associated hepatocarcinogenesis, we compared the proteomes of human NASH-associated liver biopsies, resected hepatocellular carcinomas (HCCs) and HCCs of HCV⁺ patients with normal liver tissue of patients with gastrointestinal tumor metastasis, in formalin-fixed paraffin-embedded samples obtained after surgery in our hospital during the period from 2006 to 2011. In addition, proteome analysis of liver tumors in male STAM NASH-model mice was performed...
February 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28217609/acute-pancreatitis-due-to-malaria-a-case-report-of-five-patients-and-review-of-literature
#5
Kundavaram Paul Prabhakar Abhilash, Atif Shaikh Iqbal Ahmed, Sowmya Sathyendra, Ooriapadickal Cherian Abraham
Malaria is endemic in large parts of India and can cause multiorgan failure and death. Acute pancreatitis as a complication is rare and is potentially fatal. This case series describes five adult patients between 2005 and 2010 who presented with a short duration febrile illness and diagnosed to have malaria with acute pancreatitis. The mean age of the five patients with acute pancreatitis was 40.4 years and four of them were males. None of them were alcohol consumers and did not have any other risk factor for acute pancreatitis...
July 2016: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/28216567/wine-flavonoids-in-health-and-disease-prevention
#6
Iva Fernandes, Rosa Pérez-Gregorio, Susana Soares, Nuno Mateus, Victor de Freitas
Wine, and particularly red wine, is a beverage with a great chemical complexity that is in continuous evolution. Chemically, wine is a hydroalcoholic solution (~78% water) that comprises a wide variety of chemical components, including aldehydes, esters, ketones, lipids, minerals, organic acids, phenolics, soluble proteins, sugars and vitamins. Flavonoids constitute a major group of polyphenolic compounds which are directly associated with the organoleptic and health-promoting properties of red wine. However, due to the insufficient epidemiological and in vivo evidences on this subject, the presence of a high number of variables such as human age, metabolism, the presence of alcohol, the complex wine chemistry, and the wide array of in vivo biological effects of these compounds suggest that only cautious conclusions may be drawn from studies focusing on the direct effect of wine and any specific health issue...
February 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28214241/improvement-of-operational-stability-of-ogataea-minuta-carbonyl-reductase-for-chiral-alcohol-production
#7
Kohsuke Honda, Mizuha Inoue, Tomohiro Ono, Kenji Okano, Yasumasa Dekishima, Hiroshi Kawabata
Directed evolution of enantio-selective carbonyl reductase from Ogataea minuta was conducted to improve the operational stability of the enzyme. A mutant library was constructed by an error-prone PCR and screened using a newly developed colorimetric assay. The stability of a mutant with two amino acid substitutions was significantly higher than that of the wild type at 50°C in the presence of dimethyl sulfoxide. Site-directed mutagenesis analysis showed that the improved stability of the enzyme can be attributed to the amino acid substitution of V166A...
February 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28212301/the-phytocomplex-from-fucus-vesiculosus-and-ascophyllum-nodosum-controls-postprandial-plasma-glucose-levels-an-in-vitro-and-in-vivo-study-in-a-mouse-model-of-nash
#8
Daniela Gabbia, Stefano Dall'Acqua, Iole Maria Di Gangi, Sara Bogialli, Valentina Caputi, Laura Albertoni, Ilaria Marsilio, Nicola Paccagnella, Maria Carrara, Maria Cecilia Giron, Sara De Martin
Edible seaweeds have been consumed by Asian coastal communities since ancient times. Fucus vesiculosus and Ascophyllum nodosum extracts have been traditionally used for the treatment of obesity and several gastrointestinal diseases. We evaluated the ability of extracts obtained from these algae to inhibit the digestive enzymes α-amylase and α-glucosidase in vitro, and control postprandial plasma glucose levels in a mouse model of non-alcoholic steatohepatitis (NASH); a liver disease often preceding the development of Type 2 diabetes (T2DM)...
February 15, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28211103/impaired-learning-and-memory-in-rats-induced-by-a-high-fat-diet-involvement-with-the-imbalance-of-nesfatin-1-abundance-and-copine-6-expression
#9
Zheng Chen, Ya-Yun Xu, Rui Wu, Yin-Xiu Han, Yue Yu, Jin-Fang Ge, Fei-Hu Chen
Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease, resulting not only in liver dysfunction, glucose and lipid metabolism disorder, but also in neuropsychiatric damage. In the present study, a NAFLD rat model was established via feeding of a high-fat diet, and the behavior was observed via the open field test (OFT), the sucrose preference test (SPT), the elevated plus maze (EPM), the forced swimming test (FST), and the Morris water maze (MWM). The plasma concentrations of alanine aminotransferase (ALT), glucose, free fatty acid (FFA), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were detected using chemiluminescence technique...
February 17, 2017: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/28205618/ald5-pad1-atf1-and-atf2-facilitate-the-catabolism-of-coniferyl-aldehyde-ferulic-acid-and-p-coumaric-acid-in-saccharomyces-cerevisiae
#10
Peter Temitope Adeboye, Maurizio Bettiga, Lisbeth Olsson
The ability of Saccharomyces cerevisiae to catabolize phenolic compounds remains to be fully elucidated. Conversion of coniferyl aldehyde, ferulic acid and p-coumaric acid by S. cerevisiae under aerobic conditions was previously reported. A conversion pathway was also proposed. In the present study, possible enzymes involved in the reported conversion were investigated. Aldehyde dehydrogenase Ald5, phenylacrylic acid decarboxylase Pad1, and alcohol acetyltransferases Atf1 and Atf2, were hypothesised to be involved...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28203453/association-of-osteoprotegerin-with-obesity-insulin-resistance-and-non-alcoholic-fatty-liver-disease-in-children
#11
Meltem Erol, Ozlem Bostan Gayret, Hikmet Tekin Nacaroglu, Ozgul Yigit, Oguzhan Zengi, Mehmet Salih Akkurt, Mehmet Tasdemir
BACKGROUND: Osteoprotegerin (OPG) is a member of the tumor necrosis factor superfamily. Reduced OPG levels are related to obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). OBJECTIVES: The aim of this study was to evaluate the relationship between OPG levels, obesity, insulin resistance, and NAFLD in pediatric patients. METHODS: This was a prospective, cross-sectional, controlled study that was conducted in the department of pediatrics at Bagcilar training and research hospital in Istanbul, Turkey, between April and August 2015...
November 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28199953/evaluation-of-single-and-stack-membraneless-enzymatic-fuel-cells-based-on-ethanol-in-simulated-body-fluids
#12
J Galindo-de-la-Rosa, N Arjona, A Moreno-Zuria, E Ortiz-Ortega, M Guerra-Balcázar, J Ledesma-García, L G Arriaga
The purpose of this work is to evaluate single and double-cell membraneless microfluidic fuel cells (MMFCs) that operate in the presence of simulated body fluids SBF, human serum and blood enriched with ethanol as fuels. The study was performed using the alcohol dehydrogenase enzyme immobilised by covalent binding through an array composed of carbon Toray paper as support and a layer of poly(methylene blue)/tetrabutylammonium bromide/Nafion and glutaraldehyde (3D bioanode electrode). The single MMFC was tested in a hybrid microfluidic fuel cell using Pt/C as the cathode...
February 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28197768/expression-of-an-acid-urease-with-urethanase-activity-in-e-coli-and-analysis-of-urease-gene
#13
REVIEW
Xiaofeng Liu, Qian Zhang, Nandi Zhou, Yaping Tian
Urea in alcoholic beverage is a precursor of ethyl carbamate (EC), which is carcinogenic. Enzymatic elimination of urea has attracted much research interest. Acid urease with good tolerance toward ethanol and acid is ideal enzyme for such applications. In the present work, the structural genes of urease from Providencia rettgeri JN-B815, ureABC were efficiently expressed in E. coli BL21(DE3) in an active form (apourease) exhibiting both urease and urethanase (hydrolyze EC) activities. The specific activities of the purified apourease were comparatively low, which were 2...
February 14, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28194879/ethanol-production-by-the-hyperthermophilic-archaeon-pyrococcus-furiosus-by-expression-of-bacterial-bifunctional-alcohol-dehydrogenases
#14
Matthew W Keller, Gina L Lipscomb, Diep M Nguyen, Alexander T Crowley, Gerrit J Schut, Israel Scott, Robert M Kelly, Michael W W Adams
Ethanol is an important target for the renewable production of liquid transportation fuels. It can be produced biologically from pyruvate, via pyruvate decarboxylase, or from acetyl-CoA, by alcohol dehydrogenase E (AdhE). Thermophilic bacteria utilize AdhE, which is a bifunctional enzyme that contains both acetaldehyde dehydrogenase and alcohol dehydrogenase activities. Many of these organisms also contain a separate alcohol dehydrogenase (AdhA) that generates ethanol from acetaldehyde, although the role of AdhA in ethanol production is typically not clear...
February 14, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28194561/xanthine-alkaloids-occurrence-biosynthesis-and-function-in-plants
#15
Hiroshi Ashihara, Kouichi Mizuno, Takao Yokota, Alan Crozier
Caffeine is a xanthine alkaloid found in non-alcoholic beverages such as tea, coffee, and cocoa. It was discovered in tea and coffee in the 1820s, but it was not until 2000 that details of molecular events associated with caffeine biosynthesis began to be unraveled. Reviewed are the occurrence of xanthine alkaloids in the plant kingdom and the elucidation of the caffeine biosynthesis pathway, providing details of the N-methyltransferases, belonging to the motif B' methyltransferase family, which catalyze three steps in the four-step pathway leading from xanthosine to caffeine...
2017: Progress in the Chemistry of Organic Natural Products
https://www.readbyqxmd.com/read/28194398/sphincter-of-oddi-function-and-risk-factors-for-dysfunction
#16
REVIEW
Elham Afghani, Simon K Lo, Paul S Covington, Brooks D Cash, Stephen J Pandol
The sphincter of Oddi (SO) is a smooth muscle valve regulating the flow of biliary and pancreatic secretions into the duodenum, initially described in 1887 by the Italian anatomist, Ruggero Oddi. SO dysfunction (SOD) is a broad term referring to numerous biliary, pancreatic, and hepatic disorders resulting from spasms, strictures, and relaxation of this valve at inappropriate times. This review brings attention to various factors that may increase the risk of SOD, including but not limited to: cholecystectomy, opiates, and alcohol...
2017: Frontiers in Nutrition
https://www.readbyqxmd.com/read/28193355/a-paper-based-nanomodified-electrochemical-biosensor-for-ethanol-detection-in-beers
#17
Stefano Cinti, Mattia Basso, Danila Moscone, Fabiana Arduini
Herein, we report the first example of a paper-based screen-printed biosensor for the detection of ethanol in beer samples. Common office paper was adopted to fabricate the analytical device. The properties of this paper-based screen-printed electrode (SPE) were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy, and they were compared with the well-established polyester-based SPEs as well. Paper demonstrated similar properties when compared with polyester, highlighting suitability towards its utilization in sensor development, with the advantages of low cost and simple disposal by incineration...
April 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28190676/insights-into-temperature-modulation-of-the-eucalyptus-globulus-and-eucalyptus-grandis-antioxidant-and-lignification-subproteomes
#18
Marília Gabriela de Santana Costa, Paulo Mazzafera, Tiago Santana Balbuena
Eucalyptus grandis and Eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas, as E. grandis is mostly cultivated in tropical regions while E. globulus is preferred in temperate areas. As temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes in the antioxidant system and the lignification metabolism in plantlets grown at different temperatures. Our strategy allowed the identification of 3111 stem proteins...
February 9, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28190577/dilated-cardiomyopathy
#19
REVIEW
Robert G Weintraub, Christopher Semsarian, Peter Macdonald
Dilated cardiomyopathy is defined by the presence of left ventricular dilatation and contractile dysfunction. Genetic mutations involving genes that encode cytoskeletal, sarcomere, and nuclear envelope proteins, among others, account for up to 35% of cases. Acquired causes include myocarditis and exposure to alcohol, drugs and toxins, and metabolic and endocrine disturbances. The most common presenting symptoms relate to congestive heart failure, but can also include circulatory collapse, arrhythmias, and thromboembolic events...
February 9, 2017: Lancet
https://www.readbyqxmd.com/read/28186391/a-sustainable-one-pot-two-enzymes-synthesis-of-naturally-occurring-arylalkyl-glucosides
#20
Ivan Bassanini, Jana Krejzova, Walter Panzeri, Daniela Monti, Vladimir Kren, Sergio Riva
A sustainable, convenient and scalable one-pot two-enzymes method for the glucosylation of arylalkyl alcohols has been developed. The reaction scheme is based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap commercially available natural flavonoid rutin as glycosyl donor, followed by a selective 'trimming' of the rutinoside unit catalyzed by a rhamnosidase from A. terreus. The process has been validated with the syntheses of several natural bioactive glucosides, that could be isolated in up to 75 % yield avoiding silica gel chromatography...
February 10, 2017: ChemSusChem
keyword
keyword
42566
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"