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https://www.readbyqxmd.com/read/28543189/rapid-radiations-of-both-kiwifruit-hybrid-lineages-and-their-parents-shed-light-on-a-two-layer-mode-of-species-diversification
#1
Yifei Liu, Dawei Li, Qiong Zhang, Chi Song, Caihong Zhong, Xudong Zhang, Ying Wang, Xiaohong Yao, Zupeng Wang, Shaohua Zeng, Ying Wang, Yangtao Guo, Shuaibin Wang, Xinwei Li, Li Li, Chunyan Liu, Honour C McCann, Weiming He, Yan Niu, Min Chen, Liuwen Du, Junjie Gong, Paul M Datson, Elena Hilario, Hongwen Huang
Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting the patterns of phylogenetic networks for lineages that have undergone interspecific gene flow is challenging. Here we sequenced 25 taxa representing natural diversity in the genus Actinidia with an average mapping depth of 26× on the reference genome to reconstruct their reticulate history. We found evidence, including significant gene tree discordance, cytonuclear conflicts, and changes in genome-wide heterozygosity across taxa, collectively supporting extensive reticulation in the genus...
May 25, 2017: New Phytologist
https://www.readbyqxmd.com/read/28493227/-complementary-and-alternative-procedures-for-fibromyalgia-syndrome-updated-guidelines-2017-and-overview-of-systematic-review-articles
#2
J Langhorst, P Heldmann, P Henningsen, K Kopke, L Krumbein, H Lucius, A Winkelmann, B Wolf, W Häuser
BACKGROUND: The regular update of the guidelines on fibromyalgia syndrome, AWMF number 145/004, was scheduled for April 2017. METHODS: The guidelines were developed by 13 scientific societies and 2 patient self-help organizations coordinated by the German Pain Society. Working groups (n =8) with a total of 42 members were formed balanced with respect to gender, medical expertise, position in the medical or scientific hierarchy and potential conflicts of interest...
May 10, 2017: Der Schmerz
https://www.readbyqxmd.com/read/28473802/the-subiculum-a-potential-site-of-ictogenesis-in-a-neonatal-seizure-model
#3
Xin-Xin Wang, Yong-Hua Li, Hai-Qing Gong, Pei-Ji Liang, Pu-Ming Zhang, Qin-Chi Lu
Studies have reported that the subiculum is one origin of interictal-like discharges in adult patients with temporal lobe epilepsy; however, whether the subiculum represents a site of ictogenesis for neonatal seizures remains unclear. In this study, multi-electrode recording techniques were used to record epileptiform discharges induced by low-Mg(2+) or high-K(+) artificial cerebrospinal fluid in neonatal mouse hippocampal slices, and the spatiotemporal dynamics of the epileptiform discharges were analyzed...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28468068/-hiv-infection-related-behaviors-and-preventive-measures-among-unmarried-youths-from-the-rural-areas-in-liangshan-prefecture-2011-2013
#4
S H Zhang, M Luo, S J Yang, Q Liao, Q X Wang, G Yu, L Xiao, Y H Gong, K Wang, J Wang, S C Bian, Q Liu
Objective: To analyze the HIV-infection related behaviors among unmarried youths in rural areas of Liangshan prefecture from 2011 to 2013 and factors that influencing the HIV transmission in the population under research. Methods: According to the HIV sentinel surveillance methods for data collection, EpiData 3.1 and SPSS 19.0 were used to handle data and both Chi-square test and logistic regression were used to explore the related factors. Results: A total number of 5 871 qualified youths were involved in this study from 2011 to 2013 with the prevalence rate on HIV infection among those unmarried youths from the rural areas as 3...
April 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28465464/inflammatory-signals-from-photoreceptor-modulate-pathological-retinal-angiogenesis-via-c-fos
#5
Ye Sun, Zhiqiang Lin, Chi-Hsiu Liu, Yan Gong, Raffael Liegl, Thomas W Fredrick, Steven S Meng, Samuel B Burnim, Zhongxiao Wang, James D Akula, William T Pu, Jing Chen, Lois E H Smith
Pathological neovessels growing into the normally avascular photoreceptors cause vision loss in many eye diseases, such as age-related macular degeneration and macular telangiectasia. Ocular neovascularization is strongly associated with inflammation, but the source of inflammatory signals and the mechanisms by which these signals regulate the disruption of avascular privilege in photoreceptors are unknown. In this study, we found that c-Fos, a master inflammatory regulator, was increased in photoreceptors in a model of pathological blood vessels invading photoreceptors: the very low-density lipoprotein receptor-deficient (Vldlr(-/-) ) mouse...
May 2, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28428147/high-genetic-diversities-between-isolates-of-the-fish-parasite-cryptocaryon-irritans-ciliophora-suggest-multiple-cryptic-species
#6
Hongshu Chi, Patricia Taik, Emily J Foley, Alycia C Racicot, Hilary M Gray, Katherine E Guzzetta, Hsin-Yun Lin, Yen-Ling Song, Che-Huang Tung, Kosuke Zenke, Tomoyoshi Yoshinaga, Chao-Yin Cheng, Wei-Jen Chang, Hui Gong
The ciliate protozoan Cryptocaryon irritans parasitizes marine fish and causes lethal white spot disease. Sporadic infections as well as large-scale outbreaks have been reported globally and the parasite's broad host range poses particular threat to the aquaculture and ornamental fish markets. In order to better understand C. irritans' population structure, we sequenced and compared mitochondrial cox-1, SSU rRNA, and ITS-1 sequences from 8 new isolates of C. irritans collected in China, Japan, and Taiwan. We detected two SSU rRNA haplotypes, which differ at three positions, separating the isolates into two main groups (I and II)...
April 18, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28427077/mybl2-is-a-potential-prognostic-marker-that-promotes-cell-proliferation-in-gallbladder-cancer
#7
Hai-Bin Liang, Yang Cao, Qiang Ma, Yi-Jun Shu, Zheng Wang, Fei Zhang, Yuan-Yuan Ye, Huai-Feng Li, Shan-Shan Xiang, Xiao-Ling Song, Yi Xu, Yi-Chi Zhang, Run-Fa Bao, Rui-Yan Yuan, Yi-Jian Zhang, Yun-Ping Hu, Lin Jiang, Mao-Lan Li, Xu-An Wang, Xiang-Song Wu, Wen-Guang Wu, Shuai Zhao, Yong Fand, Xiao-Peng Cui, Yun-Shu Lu, Jian Zhou, Lei Zheng, Wei Gong, Ying-Bin Liu
BACKGROUND: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. METHODS: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice...
April 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28417978/efficient-heat-dissipation-of-uncooled-400-gbps-16%C3%A3-25-gbps-optical-transceiver-employing-multimode-vcsel-and-pd-arrays
#8
Tien-Tsorng Shih, Yu-Chieh Chi, Ruei-Nian Wang, Chao-Hsin Wu, Jian-Jang Huang, Jau-Ji Jou, Tai-Cheng Lee, Hao-Chung Kuo, Gong-Ru Lin, Wood-Hi Cheng
An effective heat dissipation of uncooled 400-Gbps (16×25-Gbps) form-factor pluggable (CDFP) optical transceiver module employing chip-on-board multimode 25-Gbps vertical-surface-emitting-laser (VCSEL) and 25-Gbps photodiode (PD) arrays mounted on a brass metal core embedded within a printed circuit board (PCB) is proposed and demonstrated. This new scheme of the hollow PCB filling with thermally-dissipated brass metal core was simulated and used for high temperature and long term stability operation of the proposed 400-Gbps CDFP transceiver...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28401282/differentiation-of-cancerous-and-inflammatory-colorectal-perforations-using-multi-detector-computed-tomography
#9
Xu Hua Gong, Zhi Guo Zhuang, Jiong Zhu, Qi Feng, Jian Rong Xu, Li Jun Qian
PURPOSE: To determine reliable CT features to distinguish cancerous from inflammatory colorectal perforations. MATERIALS AND METHODS: A total of 43 patients with surgically and pathologically confirmed colorectal perforation caused by either colorectal cancer (n =27) or an inflammatory conditions (n = 16) were identified. Two radiologists independently assessed the contrast-enhanced CT features for locations of perforation, mural configurations, soft-tissue alterations, lymphadenopathy, and metastases...
April 11, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28387651/the-contribution-of-matrix-metalloproteinase-1-genotypes-to-hepatocellular-carcinoma-susceptibility-in-taiwan
#10
Yi-Liang Lai, Chi-Li Gong, Chun-Kai Fu, Te-Cheng Yueh, Chia-Wen Tsai, Wen-Shin Chang, Chieh-Lun Hsiao, Shiou-Ting Yen, Hsin-Ting Li, Long-Bin Jeng, Shou-Cheng Wang, DA-Tian Bau
BACKGROUND/AIM: Metalloproteinases (MMPs) are a family of proteases which have been shown to be overexpressed in various types of cancers. However, the contribution of MMP1 genotype to hepatocellular carcinoma (HCC) has not been well studied. This study aimed to evaluate the contribution of MMP1 promoter 1607 genotype to the risk of HCC in Taiwan, where HCC incidence is relatively high in the world. MATERIALS AND METHODS: In this case-control study, MMP1 genotype and its interaction with consumption of cigarettes and alcohol in determining HCC risk was investigated among 298 HCC patients and 889 age- and gender-matched healthy controls...
March 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28377060/bioactive-terpenoids-from-silybum-marianum-and-their-suppression-on-no-release-in-lps-induced-bv-2-cells-and-interaction-with-inos
#11
Ning-Bo Qin, Sheng-Ge Li, Xue-Yuan Yang, Chi Gong, Xiang-Yu Zhang, Jian Wang, Da-Hong Li, Yuan-Qiang Guo, Zhan-Lin Li, Hui-Ming Hua
Three new (1-3) and one known (4) bioactive terpenoids were isolated from the seeds of Silybum marianum based on the investigation to get new NO inhibitors. Their structures were determined by extensive NMR (1D and 2D NMR) and MS spectroscopic data, and the absolute configurations were identified by experimental and calculated ECD spectra. The NO inhibitory activities in murine microglial BV-2 cells and interactions with iNOS protein by molecular docking were evaluated for all compounds. The results showed that these compounds had potent NO inhibitory effects...
March 24, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28374971/a-large-single-center-real-world-study-of-clinicopathological-characteristics-and-treatment-in-advanced-alk-positive-non-small-cell-lung-cancer
#12
Gang Chen, Xi Chen, Yaxiong Zhang, Fang Yan, Wenfeng Fang, Yunpeng Yang, Shaodong Hong, Siyu Miao, Manli Wu, Xiaodan Huang, Youli Luo, Cong Zhou, Run Gong, Yan Huang, Ningning Zhou, Hongyun Zhao, Li Zhang
Crizotinib has achieved astonishing success in advanced non-small-cell lung cancer (NSCLC) patients harboring anaplastic lymphoma kinase (ALK) rearrangement. However, no real-world studies described the clinicopathological characteristics and treatment of such patients in China. Patients were consecutively collected from Sun Yat-sen University Cancer Center. Chi-square test was applied to explore the relationship between ALK fusion status and metastasis sites. Kaplan-Meier methods and multivariable analyses were used to estimate progression-free survival (PFS)...
May 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28373097/sema3f-protects-against-subretinal-neovascularization-in-vivo
#13
Ye Sun, Raffael Liegl, Yan Gong, Anima Bühler, Bertan Cakir, Steven S Meng, Samuel B Burnim, Chi-Hsiu Liu, Tristan Reuer, Peipei Zhang, Johanna M Walz, Franziska Ludwig, Clemens Lange, Hansjürgen Agostini, Daniel Böhringer, Günther Schlunck, Lois E H Smith, Andreas Stahl
Pathological neovascularization of the outer retina is the hallmark of neovascular age-related macular degeneration (nAMD). Building on our previous observations that semaphorin 3F (Sema3f) is expressed in the outer retina and demonstrates anti-angiogenic potential, we have investigated whether Sema3f can be used to protect against subretinal neovascularization in two mouse models. Both in the very low-density lipid-receptor knockout (Vldlr(-/-)) model of spontaneous subretinal neovascularization as well as in the mouse model of laser-induced choroidal neovascularization (CNV), we found protective effects of Sema3f against the formation of pathologic neovascularization...
April 2017: EBioMedicine
https://www.readbyqxmd.com/read/28243797/investigation-into-the-association-between-p2rx7-gene-polymorphisms-and-susceptibility-to-primary-gout-and-hyperuricemia-in-a-chinese-han-male-population
#14
Ying Ying, Yong Chen, Zhen Li, Haiyan Huang, Qiongyao Gong
The purpose of this study was to investigate the relationship between P2RX7 gene single nucleotide polymorphisms and primary gout and hyperuricemia in a Chinese Han male population. The genetic distributions of the single nucleotide polymorphisms (SNPs) rs2230911, rs208294, rs435309, rs28360447, rs1718119, rs28360457, and rs3751143 in P2RX7 were detected in 293 primary gout patients, 187 hyperuricemia patients and 269 controls using SNaPshot technology. Statistical analyses were implemented using SPSS version 20...
February 27, 2017: Rheumatology International
https://www.readbyqxmd.com/read/28218209/clinical-study-of-acute-vasoreactivity-testing-in-patients-with-chronic-thromboembolic-pulmonary-hypertension
#15
Qi-Xia Xu, Yuan-Hua Yang, Jie Geng, Zhen-Guo Zhai, Juan-Ni Gong, Ji-Feng Li, Xiao Tang, Chen Wang
BACKGROUND: The clinical significance of acute vasoreactivity testing (AVT) in patients with chronic thromboembolic pulmonary hypertension (CTEPH) remains unclear. We analyzed changes in hemodynamics and oxygenation dynamics indices after AVT in patients with CTEPH using patients with pulmonary arterial hypertension (PAH) as controls. METHODS: We analyzed retrospectively the results of AVT in 80 patients with PAH and 175 patients with CTEPH registered in the research database of Beijing Chao-Yang Hospital between October 2005 and August 2014...
February 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28199833/fgf21-administration-suppresses-retinal-and-choroidal-neovascularization-in-mice
#16
Zhongjie Fu, Yan Gong, Raffael Liegl, Zhongxiao Wang, Chi-Hsiu Liu, Steven S Meng, Samuel B Burnim, Nicholas J Saba, Thomas W Fredrick, Peyton C Morss, Ann Hellstrom, Saswata Talukdar, Lois E H Smith
Pathological neovascularization, a leading cause of blindness, is seen in retinopathy of prematurity, diabetic retinopathy, and age-related macular degeneration. Using a mouse model of hypoxia-driven retinal neovascularization, we find that fibroblast growth factor 21 (FGF21) administration suppresses, and FGF21 deficiency worsens, retinal neovessel growth. The protective effect of FGF21 against neovessel growth was abolished in adiponectin (APN)-deficient mice. FGF21 administration also decreased neovascular lesions in two models of neovascular age-related macular degeneration: very-low-density lipoprotein-receptor-deficient mice with retinal angiomatous proliferation and laser-induced choroidal neovascularization...
February 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28186925/a-critical-appraisal-of-the-methodology-and-quality-of-evidence-of-systematic-reviews-and-meta-analyses-of-traditional-chinese-medical-nursing-interventions-a-systematic-review-of-reviews
#17
Ying-Hui Jin, Guo-Hao Wang, Yi-Rong Sun, Qi Li, Chen Zhao, Ge Li, Jin-Hua Si, Yan Li, Cui Lu, Hong-Cai Shang
OBJECTIVE: To assess the methodology and quality of evidence of systematic reviews and meta-analyses of traditional Chinese medical nursing (TCMN) interventions in Chinese journals. These interventions include acupressure, massage, Tai Chi, Qi Gong, electroacupuncture and use of Chinese herbal medicines-for example, in enemas, foot massage and compressing the umbilicus. DESIGN: A systematic literature search for systematic reviews and meta-analyses of TCMN interventions was performed...
November 14, 2016: BMJ Open
https://www.readbyqxmd.com/read/28181684/exercise-based-cardiac-rehabilitation-for-adults-with-atrial-fibrillation
#18
REVIEW
Signe S Risom, Ann-Dorthe Zwisler, Pernille P Johansen, Kirstine L Sibilitz, Jane Lindschou, Christian Gluud, Rod S Taylor, Jesper H Svendsen, Selina K Berg
BACKGROUND: Exercise-based cardiac rehabilitation may benefit adults with atrial fibrillation or those who had been treated for atrial fibrillation. Atrial fibrillation is caused by multiple micro re-entry circuits within the atrial tissue, which result in chaotic rapid activity in the atria. OBJECTIVES: To assess the benefits and harms of exercise-based rehabilitation programmes, alone or with another intervention, compared with no-exercise training controls in adults who currently have AF, or have been treated for AF...
February 9, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28127799/tbl1xr1-predicts-isolated-tumor-cells-and-micrometastasis-in-patients-with-tnm-stage-i-ii-colorectal-cancer
#19
Hongda Liu, Zhaochen Liu, Kangshuai Li, Shuo Li, Lei Song, Zheng Gong, Weichen Shi, Hui Yang, Yunfei Xu, Shanglei Ning, Sayed Ismail, Yuxin Chen
BACKGROUND AND AIM: A considerable number of early stage colorectal cancer (CRC) patients may develop cancer relapse or metastasis after curative surgery. Isolated tumor cells (ITC) and micrometastasis in lymph nodes (LNMM), which are undetectable by conventional pathological examination, may be one primary reason. Detection of ITC/LNMM is time-consuming and cost-ineffective, we aimed to find biomarkers in primary CRC tissues to help predicting ITC/LNMM status. METHODS: We enrolled 137 node-negative patients with early stage CRC in this study...
January 27, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28127060/tricolor-r-g-b-laser-diode-based-eye-safe-white-lighting-communication-beyond-8%C3%A2-gbit-s
#20
Tsai-Chen Wu, Yu-Chieh Chi, Huai-Yung Wang, Cheng-Ting Tsai, Yu-Fang Huang, Gong-Ru Lin
White light generation by mixing red, green, and blue laser diodes (RGB LDs) was demonstrated with Commission International de l'Eclairage coordinates of (0.2928, 0.2981), a correlated color temperature of 8382 K, and a color rendering index of 54.4 to provide a maximal illuminance of 7540 lux. All the white lights generated using RGB LDs were set within the risk group-1 criterion to avoid the blue-light hazard to human eyes. In addition, the RGB-LD mixed white light was diffused using a frosted glass to avoid optical aberration and to improve the performance of the lighting source...
December 2017: Scientific Reports
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