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Autofluorescence

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https://www.readbyqxmd.com/read/28735528/lipids-evergreen-autofluorescent-biomarkers-for-the-liver-functional-profiling
#1
Anna Cleta Croce, Giovanni Bottiroli
Depending on their chemical nature, lipids can be classified in two main categories: hydrophilic, greatly contributing to membrane composition and subcellular organelle compartmentalization, and hydrophobic, mostly triglycerides, greatly enrolled in the storage and production of energy. In both cases, some lipid molecules can be involved as signaling agents in the regulation of metabolism and protective or damaging pathways in responses to harmful stimuli. These events could affect in particular the liver, because of its central role in the maintenance of lipid homeostasis...
June 1, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28733050/the-optical-imaging-of-an-idiopathic-paracentral-acute-middle-maculopathy-chinese-young-man-and-review-of-the-literatures
#2
REVIEW
Yanyan Chen, Yuedong Hu
We report a case of idiopathic paracentral acute middle maculopathy with typical optical imaging and clinical features. A 26-year-old Chinese policeman presented with a 20-day history of paracentral scotoma in the left eye after early wake-up and long-time working in front of computer the day before. He experienced sudden-onset strong flash in his left eye. Paracentral scotoma appeared in his left eye after 6hours of rest. The general history was negative. His best-corrected visual acuity was 20/20 OU. Anterior segment, dilated fundus examination, papillary evaluation and intraocular pressure were normal...
July 18, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28732589/accuracy-of-diagnostic-imaging-modalities-for-classifying-pediatric-eyes-as-papilledema-versus-pseudopapilledema
#3
Melinda Y Chang, Federico G Velez, Joseph L Demer, Laura Bonelli, Peter A Quiros, Anthony C Arnold, Alfredo A Sadun, Stacy L Pineles
PURPOSE: To identify the most accurate diagnostic imaging modality for classifying pediatric eyes as papilledema (PE) or pseudopapilledema (PPE). DESIGN: Prospective observational study. SUBJECTS: Nineteen children between the ages of 5 and 18 years were recruited. Five children (10 eyes) with PE, 11 children (19 eyes) with PPE owing to suspected buried optic disc drusen (ODD), and 3 children (6 eyes) with PPE owing to superficial ODD were included...
July 18, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28729371/geographic-atrophy-phenotype-identification-by-cluster-analysis
#4
Jordi Monés, Marc Biarnés
BACKGROUND/AIMS: To identify ocular phenotypes in patients with geographic atrophy secondary to age-related macular degeneration (GA) using a data-driven cluster analysis. METHODS: This was a retrospective analysis of data from a prospective, natural history study of patients with GA who were followed for ≥6 months. Cluster analysis was used to identify subgroups within the population based on the presence of several phenotypic features: soft drusen, reticular pseudodrusen (RPD), primary foveal atrophy, increased fundus autofluorescence (FAF), greyish FAF appearance and subfoveal choroidal thickness (SFCT)...
July 20, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28729369/pigmentary-retinopathy-rod-cone-dysfunction-and-sensorineural-deafness-associated-with-a-rare-mitochondrial-trna-lys-m-8340g-a-gene-variant
#5
Jaidip S Gill, Steven A Hardy, Emma L Blakely, Sila Hopton, Andrea H Nemeth, Carl Fratter, Joanna Poulton, Robert W Taylor, Susan M Downes
BACKGROUND/AIM: The rare mitochondrial DNA (mtDNA) variant m.8340G>A has been previously reported in the literature in a single, sporadic case of mitochondrial myopathy. In this report, we aim to investigate the case of a 39-year-old male patient with sensorineural deafness who presented to the eye clinic with nyctalopia, retinal pigmentary changes and bilateral cortical cataracts. METHODS: The patient was examined clinically and investigated with autofluorescence, full-field electroretinography, electro-oculogram and dark adaptometry...
July 20, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728169/correlations-between-macular-skin-and-serum-carotenoids
#6
Christopher D Conrady, James P Bell, Brian M Besch, Aruna Gorusupudi, Kelliann Farnsworth, Igor Ermakov, Mohsen Sharifzadeh, Maia Ermakova, Werner Gellermann, Paul S Bernstein
Purpose: Ocular and systemic measurement and imaging of the macular carotenoids lutein and zeaxanthin have been employed extensively as potential biomarkers of AMD risk. In this study, we systematically compare dual wavelength retinal autofluorescence imaging (AFI) of macular pigment with skin resonance Raman spectroscopy (RRS) and serum carotenoid levels in a clinic-based population. Methods: Eighty-eight patients were recruited from retina and general ophthalmology practices from a tertiary referral center and excluded only if they did not have all three modalities tested, had a diagnosis of macular telangiectasia (MacTel) or Stargardt disease, or had poor AFI image quality...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28726568/double-hyperautofluorescent-ring-on-fundus-autofluorescence-in-abca4
#7
Maria Fernanda Abalem, Cynthia X Qian, Kari Branham, Dana Schlegel, Abigail T Fahim, Naheed W Khan, John R Heckenlively, K Thiran Jayasundera
We report an unusual phenotype in a child with a clinical diagnosis of recessive Stargardt disease (STGD1) and two pathogenic variants in the ABCA4 gene. Typically, the diagnosis of early-onset STGD1 is challenging because children may present with a variety of fundus changes and a variable rate of progression. At the time of his initial visit, the 6-year-old boy presented with 20/200 OD (right eye) and 20/150 OS (left eye), symmetrical mild foveal atrophy without flecks on fundus exam, and foveal hypoautofluorescence surrounded by a homogeneous hyperautofluorescent background on wide-field fundus autofluorescence...
July 20, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/28719154/progression-of-skin-autofluorescence-of-ages-over-4-years-in-patients-with-type-1-diabetes
#8
Kalina Rajaobelina, Catherine Helmer, Fritz-Line Vélayoudom-Céphise, Sovanndany Nov, Blandine Farges, Emilie Pupier, Laurence Blanco, Marie Hugo, Henri Gin, Vincent Rigalleau
BACKGROUND: The deposit of Advanced Glycation End-products (AGEs) is involved in diabetic complications. It can be evaluated by measuring the skin autofluorescence (sAF). We searched whether sAF progressed over 4 years in Type 1 Diabetes, and analysed its relationship with the development of nephropathy. METHODS: Two measurements of sAF was realized on 154 patients during years 2009 and 2013. Baseline factors associated to the progression of sAF were analyzed by multivariate regression analysis...
July 18, 2017: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/28717585/erratum-measuring-the-effect-of-western-diet-on-liver-tissue-architecture-by-flim-autofluorescence-and-harmonic-generation-microscopy-erratum
#9
Suman Ranjit, Alexander Dvornikov, Evgenia Dobrinskikh, Xiaoxin Wang, Yuhuan Luo, Moshe Levi, Enrico Gratton
[This corrects the article on p. 3143 in vol. 8.].
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28717559/measuring-the-effect-of-a-western-diet-on-liver-tissue-architecture-by-flim-autofluorescence-and-harmonic-generation-microscopy
#10
Suman Ranjit, Alexander Dvornikov, Evgenia Dobrinskikh, Xiaoxin Wang, Yuhuan Luo, Moshe Levi, Enrico Gratton
The phasor approach to auto-fluorescence lifetime imaging was used to identify and characterize a long lifetime species (LLS) (~7.8 ns) in livers of mice fed with a Western diet. The size of the areas containing this LLS species depends on the type of diet and the size distribution shows Western diet has much larger LLS sizes. Combination of third harmonic generation images with FLIM identified the LLS species with fat droplets and the droplet size distribution was estimated. Second harmonic generation microscopy combined with phasor FLIM shows that there is an increase in fibrosis with a Western diet...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28715581/incidence-and-factors-influencing-retinal-displacement-in-eyes-treated-for-rhegmatogenous-retinal-detachment-with-vitrectomy-and-gas-or-silicone-oil
#11
Roberto dell'Omo, Andrea Scupola, Davide Viggiano, Maria Grazia Sammarco, Serena De Turris, Mario R Romano, Gabriela Grimaldi, Ermanno dell'Omo, Ciro Costagliola
Purpose: The purpose of this study was to study the incidence and factors influencing retinal displacement in eyes treated for rhegmatogenous retinal detachment (RRD) with pars plana vitrectomy (PPV) and gas or silicone oil. Methods: This was a prospective observational case series. One hundred twenty-five eyes with macula-off RRD from 125 patients underwent 25-gauge PPV at two vitreoretinal institutional practices. Eyes without proliferative vitreoretinopathy (PVR) or PVR grade A were tamponated with sulfur hexafluoride (SF6) gas, whereas eyes with PVR grade B received 1000 centistokes silicone oil (SO)...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28714046/effects-of-cd-and-zn-on-physiological-and-anatomical-properties-of-hydroponically-grown-brassica-napus-plants
#12
Martina Benáková, Hassan Ahmadi, Zuzana Dučaiová, Edita Tylová, Stephan Clemens, Jiří Tůma
Clarifying the connection between metal exposure and anatomical changes represents an important challenge for a better understanding of plant phytoextraction potential. A hydroponic screening experiment was carried out to evaluate the effects of combined interactions of Cd and Zn on mineral uptake (Mg, K, Ca, Na) and on the physiological and anatomical characteristics of Brassica napus L cv. Cadeli, Viking, and Navajo. Plants were exposed to 5 μM Cd (CdCl2), 10 μM Zn (ZnSO4), or both Cd + Zn, for 14 days...
July 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28708222/multimodal-imaging-in-a-patient-with-traumatic-choroidal-ruptures
#13
Luisa Pierro, Chiara Giuffrè, Alessandro Rabiolo, Marco Gagliardi, Alessandro Arrigo, Francesco Bandello
PURPOSE: To describe the case and the follow-up of a traumatic choroidal rupture characterized by means of multimodal imaging including color fundus photographs, infrared reflectance, blue autofluorescence, swept-source optical coherence tomography, fluorescein angiography (FA), indocyanine green angiography (ICGA), and optical coherence tomography angiography (OCT-A). METHODS: Case report. RESULTS: A 17-year-old boy was referred to our clinic complaining of reduction in visual acuity in the right eye (RE) after a blunt ocular trauma during a soccer match...
July 5, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28706756/impact-of-vital-dyes-on-cell-viability-and-transduction-efficiency-of-aav-vectors-used-in-retinal-gene-therapy-surgery-an-in-vitro-and-in-vivo-analysis
#14
Anna P Salvetti, Maria I Patrício, Alun R Barnard, Harry O Orlans, Doron G Hickey, Robert E MacLaren
PURPOSE: Treatment of inherited retinal degenerations using adeno-associated viral (AAV) vectors involves delivery by subretinal injection. In the latter stages, alteration of normal anatomy may cause difficulty in visualizing the retinotomy, retinal detachment extension, and vector diffusion. Vital dyes may be useful surgical adjuncts, but their safety and impact on AAV transduction are largely unknown. METHODS: The effects of Sodium Fluorescein (SF), Membrane Blue (MB), and Membrane Blue Dual (DB) at a range of dilutions were assessed on human embryonic kidney cells in vitro using an AAV2-green fluorescent protein (GFP) reporter at different multiplicities of infection...
July 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28706202/near-infrared-autofluorescence-induced-by-intraplaque-hemorrhage-and-heme-degradation-as-marker-for-high-risk-atherosclerotic-plaques
#15
Nay Min Htun, Yung Chih Chen, Bock Lim, Tara Schiller, Ghassan J Maghzal, Alex L Huang, Kirstin D Elgass, Jennifer Rivera, Hans G Schneider, Bayden R Wood, Roland Stocker, Karlheinz Peter
Atherosclerosis is a major cause of mortality and morbidity, which is mainly driven by complications such as myocardial infarction and stroke. These complications are caused by thrombotic arterial occlusion localized at the site of high-risk atherosclerotic plaques, of which early detection and therapeutic stabilization are urgently needed. Here we show that near-infrared autofluorescence is associated with the presence of intraplaque hemorrhage and heme degradation products, particularly bilirubin by using our recently created mouse model, which uniquely reflects plaque instability as seen in humans, and human carotid endarterectomy samples...
July 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28704777/preinvasive-disease-of-the-airway
#16
REVIEW
Ricky M Thakrar, Adam Pennycuick, Elaine Borg, Sam M Janes
Squamous cell carcinoma of the lung arises from preinvasive progenitors in the central airways. The archetypal model appears to be a stepwise morphological progression until there is invasion of the basement membrane. However, not every lesion appears to follow this course and many individuals can have stable disease, or indeed regress to normal epithelium. From our increased understanding of the molecular pathology it is becoming apparent that the respiratory epithelium accumulates progressive genetic and epigenetic insults in response to carcinogens...
July 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28702587/simple-fluorescence-sensing-of-extreme-acidity-based-on-inner-filter-effect-of-ascorbic-acid-to-fluorescent-au-nanoclusters
#17
Ronghui Zhou, Yuqin Li, Yuanyuan Tian, Qun Sun, Pengchi Deng, Lan Wu, Xiandeng Hou
As acidic pH plays essential roles in several organelles, development of pH sensors for extreme acidity is of vital importance. Herein, on the basis of the inner filter effect (IFE) between ascorbic acid (AA) and Au nano-clusters (AuNCs), a simple extreme acidity fluorescence sensor was developed without complex organic synthesis. The as-prepared probe can be used to detect acidity within the pH range of 2.4-4.6. Moreover, the probe possesses high selectivity, good photostability, and excellent reversibility for monitoring extreme acidity in bacterial cells without the influence of autofluorescence...
July 12, 2017: Nanoscale
https://www.readbyqxmd.com/read/28699929/arcuate-nerve-fiber-layer-changes-after-internal-limiting-membrane-peeling-in-idiopathic-epiretinal-membrane
#18
Andrea Scupola, Gabriela Grimaldi, Edoardo Abed, Maria G Sammarco, Andrea Giudiceandrea, Andrea Greco, Paola Sasso, Maria A Blasi
PURPOSE: To analyze the relationship between swelling of the arcuate nerve fiber layer (SANFL) and long-term decrease of retinal nerve fiber layer thickness after internal limiting membrane peeling for idiopathic epiretinal membrane, and to investigate if SANFL is related to a mechanical surgical damage. METHODS: Prospective, interventional consecutive case series of 46 eyes that underwent combined epiretinal membrane/internal limiting membrane peeling for idiopathic epiretinal membrane...
July 7, 2017: Retina
https://www.readbyqxmd.com/read/28696576/nad-p-h-and-flavin-autofluorescence-correlation-with-atp-in-rat-livers-with-different-metabolic-steady-state-conditions
#19
Anna C Croce, Andrea Ferrigno, Laura G Di Pasqua, Clarissa Berardo, Giovanni Bottiroli, Mariapia Vairetti
The monitoring of NAD(P)H and flavin autofluorescence (AF) is at the basis of numerous investigations on energy metabolism. Nevertheless, the ability of these AF biomarkers to accurately represent the energy currency, ATP, is poorly explored. Here we focused on the AF/ATP correlation in lean and fatty livers with different steady-state metabolic conditions, achieved after organ isolation, preservation and recovery, in a likely dependence on both liver intrinsic metabolic features and externally induced perturbations...
July 11, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28696566/super-resolution-imaging-of-subcortical-white-matter-using-stochastic-optical-reconstruction-microscopy-storm-and-super-resolution-optical-fluctuation-imaging-sofi
#20
Atticus H Hainsworth, Sarah Lee, Peter Foot, Anita Patel, Wayne W Poon, Alex E Knight
AIMS: The spatial resolution of light microscopy is limited by the wavelength of visible light (the "diffraction limit", approximately 250 nm). Resolution of sub-cellular structures, smaller than this limit, is possible with super resolution methods such as stochastic optical reconstruction microscopy (STORM) and super-resolution optical fluctuation imaging (SOFI). We aimed to resolve subcellular structures (axons, myelin sheaths and astrocytic processes) within intact white matter using STORM and SOFI...
July 11, 2017: Neuropathology and Applied Neurobiology
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