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Autofluorescence

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https://www.readbyqxmd.com/read/28426871/macular-pigment-density-and-quantitative-fundus-autofluorescence-in-young-healthy-subjects
#1
Chiara M Eandi, Marco Nassisi, Carlo Lavia, Camilla Alovisi, Ugo de Sanctis
Purpose: To measure macular pigment (MP) and find possible correlation between heterochromatic flicker photometry (HFP) and quantitative autofluorescence (qAF) in young healthy subjects. Methods: We enrolled 80 eyes of 40 young healthy subjects. Macular pigment optical density (MPOD) was automatically calculated with a macular pigment screener (MPS; MPODHFP). We calculated qAF comparing gray levels (GL) of qAF images with GL of internal reference of a confocal scanning laser ophthalmoscopy...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28421434/estimation-of-fluorescence-lifetime-of-lipofuscin-fluorophores-contained-in-lipofuscin-granules-of-retinal-pigment-epithelium-of-human-cadaver-eyes-without-signs-of-pathology
#2
M A Yakovleva, T B Feldman, P M Arbukhanova, S A Borzenok, V A Kuzmin, M A Ostrovsky
The fluorescence lifetimes of lipofuscin fluorophores contained in chloroform extracts from retinal pigment epithelium (RPE) of human cadaver eyes without signs of pathology were evaluated by single photon counting. The comparison of fluorescence lifetimes of N-retinylidene-N-retinylethanolamine (A2E) and its photooxidation and photodegradation products has been carried out. It was shown that the contribution of A2E to the total fluorescence of chloroform extract from lipofuscin granules is not major. The results are important for the improvement of noninvasive diagnostic method of degenerative diseases of the retina and RPE-fundus autofluorescence (FAF)...
January 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28421226/portable-led-induced-autofluorescence-spectroscopy-for-oral-cancer-diagnosis
#3
Yung-Jhe Yan, Ting-Wei Huang, Nai-Lun Cheng, Yao-Fang Hsieh, Ming-Hsui Tsai, Jin-Chern Chiou, Jeng-Ren Duann, Yung-Jiun Lin, Chin-Siang Yang, Mang Ou-Yang
No abstract text is available yet for this article.
April 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28419401/unilateral-pigmented-paravenous-retinochoroidal-atrophy-associated-with-presumed-ocular-tuberculosis
#4
Guillermo Fernandez-Sanz, Ester Carreño, Sonia Mall, Magella M Neveu, Graham E Holder, Dhanes Thomas
This report describes a case of unilateral pigmented paravenous retinochoroidal atrophy (PPRCA) in a patient with low-grade unilateral intermediate uveitis. A 31-year-old woman, previously diagnosed with intermediate uveitis in the right eye (OD) presented to the clinic. Best-corrected visual acuity was 20/20 OD. Fundus examination, fluorescein angiography, autofluorescence, and optical coherence tomography OD were in keeping with a phenotypic diagnosis of PPRCA. Electrophysiology showed severe photoreceptor dysfunction of both the rod and the cone systems OD...
April 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28412659/development-of-near-infrared-ratiometric-fluorescent-probe-based-on-cationic-conjugated-polymer-and-cdte-cds-qds-for-label-free-determination-of-glucose-in-human-body-fluids
#5
Mengze Yu, Kunli Zhao, Xiaohua Zhu, Shiyun Tang, Zhou Nie, Yan Huang, Peng Zhao, Shouzhuo Yao
Quantum dots (QDs) have attracted extensive attention in biomedical applications, because of their broad excitation spectra, narrow and symmetric emission peaks etc. Furthermore, near-infrared (NIR) QDs have further advantages including low autofluorescence, good tissue penetration and low phototoxicity. In this work, the electrostatic interaction and fluorescence resonance energy transfer (FRET) between NIR CdTe/CdS QDs and cationic conjugated polymer (CCP) was studied for the first time. Based on the newly discovered phenomena and the result that hydrogen peroxide (H2O2) can efficiently quench the fluorescence of NIR CdTe/CdS QDs, a novel NIR ratiometric fluorescent probe for determination of H2O2 and glucose was developed...
April 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28412069/whole-exome-sequencing-identifies-biallelic-idh3a-variants-as-a-cause-of-retinitis-pigmentosa-accompanied-by-pseudocoloboma
#6
Laurence H M Pierrache, Adva Kimchi, Rinki Ratnapriya, Lisa Roberts, Galuh D N Astuti, Alexey Obolensky, Avigail Beryozkin, Martha J H Tjon-Fo-Sang, Jose Schuil, Caroline C W Klaver, Ernie M H F Bongers, Lonneke Haer-Wigman, Nicoline Schalij, Martijn H Breuning, Gratia M Fischer, Eyal Banin, Raj S Ramesar, Anand Swaroop, L Ingeborgh van den Born, Dror Sharon, Frans P M Cremers
PURPOSE: To identify the genetic cause of and describe the phenotype in 4 families with autosomal recessive retinitis pigmentosa (arRP) that can be associated with pseudocoloboma. DESIGN: Case series. PARTICIPANTS: Seven patients from 4 unrelated families with arRP, among whom 3 patients had bilateral early-onset macular pseudocoloboma. METHODS: We performed homozygosity mapping and whole-exome sequencing in 5 probands and 2 unaffected family members from 4 unrelated families...
April 12, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28410977/intravital-imaging-of-the-kidney
#7
REVIEW
Takashi Hato, Seth Winfree, Pierre C Dagher
Two-photon intravital microscopy is a powerful tool that allows the examination of dynamic cellular processes in the live animal with unprecedented resolution. Indeed, it offers the ability to address unique biological questions that may not be solved by other means. While two-photon intravital microscopy has been successfully applied to study many organs, the kidney presents its own unique challenges that need to be overcome in order to optimize and validate imaging data. For kidney imaging, the complexity of renal architecture and salient autofluorescence merit special considerations as these elements directly impact image acquisition and data interpretation...
April 11, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28409558/in-vitro-detection-of-cholangiocarcinoma-cells-using-a-fluorescent-protein-expressing-oncolytic-herpes-virus
#8
R J S Coelen, M J de Keijzer, R Weijer, V V Loukachov, J K Wiggers, F P J Mul, A C W A van Wijk, Y Fong, M Heger, T M van Gulik
Pathological confirmation is desired prior to high-risk surgery for suspected perihilar cholangiocarcinoma (PHC), but preoperative tissue diagnosis is limited by poor sensitivity of available techniques. This study aimed to validate whether a tumor-specific enhanced green fluorescent protein (eGFP)-expressing oncolytic virus could be used for cholangiocarcinoma (CC) cell detection. Extrahepatic CC cell lines SK-ChA-1, EGI-1, TFK-1 and control cells (primary human liver cells) were exposed to the oncolytic herpes simplex type 1 virus NV1066 for up to 24 h in adherent culture...
April 14, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28408433/the-relationship-between-circulating-irisin-levels-and-tissues-age-accumulation-in-type-2-diabetes-patients
#9
Zhu Li, Gang Wang, Yanjuan Zhu, Chenguang Li, Yunzhao Tang, Zhenhuan Jiang, Min Yang, Changlin Ni, Li-Ming Chen, Wen-Yan Niu
Objective: AGEs, measured by skin autofluorescence (AF), are a factor in the development or worsening of many degenerative diseases, such as diabetes and atherosclerosis. Irisin levels has been associated with diabetes, endothelial dysfunction and atherosclerosis. The objective of this study was to investigate whether circulating irisin levels are correlated with skin AF values in type 2 diabetes patients. Design: A total of 362 Chinese type 2 diabetic patients and 100 age- and sex-matched healthy controls were recruited in this study...
April 13, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28405811/advanced-glycation-end-products-as-a-biomarker-for-incisional-hernia
#10
J J Harlaar, H H Eker, K A Vakalopoulos, M C Cabezas, A C van der Ham, W W Vrijland, J Jeekel, J F Lange
BACKGROUND: Incisional hernia is one of the most frequent complications after abdominal surgery, with incidences up to 30%. A reliable biomarker for the prediction of this complication is lacking. Advanced glycosylation end products (AGEs), also known as non-enzymatic collagen crosslinks, are correlated with aging, smoking, hyperglycemia, hyperlipidemia and oxidative stress. In this study the accumulation of AGEs and the relation between AGEs and incisional hernia were investigated. MATERIALS AND METHODS: In an exploratory case-control study, 23 patients with incisional hernia after midline incision were compared with 17 patients without clinical or radiological signs of incisional hernia after midline incision, AGEs were measured using a Skin Auto Fluorescence (SAF)-reader...
April 12, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28405744/long-term-follow-up-of-fellow-eye-in-patients-with-lamellar-macular-hole
#11
Ugo Nava, Matteo Giuseppe Cereda, Ferdinando Bottoni, Chiara Preziosa, Marco Pellegrini, Andrea Giani, Giovanni Staurenghi
BACKGROUND: To evaluate macular changes in fellow eyes of patients diagnosed with lamellar macular hole (LMH) using spectral-domain optical coherence tomography (SD-OCT) and blue fundus autofluorescence (B-FAF). METHODS: Fellow eyes of patients diagnosed with a LMH were retrospectively evaluated on OCT. Best-corrected visual acuity (BCVA) and central foveal thickness (CFT) were recorded. Corresponding B-FAF images, vitreo-macular relations, and type of epiretinal membranes (ERMs) were also examined...
April 13, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28400456/diverging-effects-of-diabetes-mellitus-in-patients-with-peripheral-artery-disease-and-abdominal-aortic-aneurysm-and-the-role-of-advanced-glycation-end-products-artery-study-protocol-for-a-multicentre-cross-sectional-study
#12
L C de Vos, J Boersema, J L Hillebrands, C G Schalkwijk, R Meerwaldt, J C Breek, A J Smit, C J Zeebregts, J D Lefrandt
INTRODUCTION: Diabetes mellitus is a well-defined risk factor for peripheral artery disease (PAD), but protects against the development and growth of abdominal aortic aneurysm (AAA). Diabetes mellitus is associated with arterial stiffening and peripheral arterial media sclerosis. Advanced glycation end-products (AGEs) are increased in diabetes mellitus and cardiovascular disease. AGEs are known to form cross-links between proteins and are associated with arterial stiffness. Whether AGEs contribute to the protective effects of diabetes mellitus in AAA is unknown...
April 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28395300/noninvasive-quantification-of-retinal-microglia-using-widefield-autofluorescence-imaging
#13
Despina Kokona, Nadia Schneider, Helena Giannakaki-Zimmermann, Joel Jovanovic, Andreas Ebneter, Martin Zinkernagel
Purpose: To validate widefield autofluorescence (AF) in vivo imaging of the retina in mice expressing green fluorescent protein (gfp) in microglia, and to monitor retinal microglia reconstitution in vivo after lethal irradiation and bone marrow transplantation. Methods: Transgenic Cx3cr1gfp/gfp and wildtype Balb/c mice were used in this study. A confocal scanning laser ophthalmoscope was used for AF imaging with a 55° and a widefield 102° lens. Intrasession reproducibility was assessed for each lens...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28395299/multimodal-fundus-imaging-of-sodium-iodate-treated-mice-informs-rpe-susceptibility-and-origins-of-increased-fundus-autofluorescence
#14
Jin Zhao, Hye Jin Kim, Janet R Sparrow
Purpose: By multimodal imaging, and the use of mouse and in vitro models, we have addressed changes in fundus autofluorescence (488 and 790 nm) and observed interactions between the photooxidative stress imposed by RPE bisretinoid lipofuscin and the oxidative impact of systemic sodium iodate (NaIO3). Methods: Abca4-/-, wild-type, and Rpe65rd12 mice were given systemic injections of NaIO3 (30 mg/kg). Analysis included noninvasive imaging of fundus autofluorescence (short-wavelength [SW-AF]; near-infrared excitation [NIR-AF]), quantitative fundus AF (qAF; 488 nm); light microscopy, RPE flat-mounts and measurements of outer nuclear layer (ONL) thickness...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28391446/a-review-of-current-and-future-management-of-geographic-atrophy
#15
REVIEW
Riccardo Sacconi, Eleonora Corbelli, Lea Querques, Francesco Bandello, Giuseppe Querques
Age-related macular degeneration (AMD) is a multifactorial disease and a leading cause of vision impairment in elderly people in Western society. Geographic atrophy (GA), the late stage of dry AMD, is typically defined as a round or oval area of atrophy of 175 µm or more. In GA patients, visual acuity (VA) can still be good if the macula is spared, but decreased if GA extends through the fovea causing a great impairment of quality of life. Because of a poor correlation between VA and GA lesions or progression, a multimodal imaging approach is necessary to better follow up GA patients...
April 8, 2017: Ophthalmology and Therapy
https://www.readbyqxmd.com/read/28390949/ncls-and-er-a-stressful-relationship
#16
REVIEW
Davide Marotta, Elisa Tinelli, Sara E Mole
The Neuronal Ceroid Lipofuscinoses (NCLs, Batten disease) are a group of inherited neurodegenerative disorders with variable age of onset, characterised by the lysosomal accumulation of autofluorescent ceroid lipopigments. The endoplasmic reticulum (ER) is a critical organelle for normal cell function. Alteration of ER homeostasis leads to accumulation of misfolded protein in the ER and to activation of the unfolded protein response. ER stress and the UPR have recently been linked to the NCLs. In this review, we will discuss the evidence for UPR activation in the NCLs, and address its connection to disease pathogenesis...
April 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28386970/lack-of-differentiation-of-allergic-and-irritant-reactions-by-skin-autofluorescence
#17
Eun Jae Shin, Min Jae Gwak, Ki Heon Jeong, Min Kyung Shin
No abstract text is available yet for this article.
May 2017: Contact Dermatitis
https://www.readbyqxmd.com/read/28384671/acute-zonal-cone-photoreceptor-outer-segment-loss
#18
Tomas S Aleman, Harpal S Sandhu, Leona W Serrano, Anastasia Traband, Marisa K Lau, Grazyna Adamus, Robert A Avery
Importance: The diagnostic path presented narrows down the cause of acute vision loss to the cone photoreceptor outer segment and will refocus the search for the cause of similar currently idiopathic conditions. Objective: To describe the structural and functional associations found in a patient with acute zonal occult photoreceptor loss. Design, Setting, and Participants: A case report of an adolescent boy with acute visual field loss despite a normal fundus examination performed at a university teaching hospital...
April 6, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28382556/acute-progressive-paravascular-placoid-neuroretinopathy-with-negative-type-electroretinography-in-paraneoplastic-retinopathy
#19
Fred K Chen, Avenell L Chew, Dan Zhang, Shang-Chih Chen, Enid Chelva, Erandi Chandrasekera, Eleanor M H Koay, John Forrester, Samuel McLenachan
PURPOSE: Paraneoplastic retinopathy can be the first manifestation of systemic malignancy. A subset of paraneoplastic retinopathy is characterized by negative-type electroretinography (ERG) without fundus abnormality. Here we describe the multimodal imaging and clinico-pathological correlation of a unique case of acute progressive paravascular placoid neuroretinopathy with suspected retinal depolarizing bipolar cell dysfunction preceding the diagnosis of metastatic small cell carcinoma of the prostate...
April 5, 2017: Documenta Ophthalmologica. Advances in Ophthalmology
https://www.readbyqxmd.com/read/28380997/self-interference-fluorescence-microscopy-with-three-phase-detection-for-depth-resolved-confocal-epi-fluorescence-imaging
#20
Boy Braaf, Johannes F de Boer
Three-dimensional confocal fluorescence imaging of in vivo tissues is challenging due to sample motion and limited imaging speeds. In this paper a novel method is therefore presented for scanning confocal epi-fluorescence microscopy with instantaneous depth-sensing based on self-interference fluorescence microscopy (SIFM). A tabletop epi-fluorescence SIFM setup was constructed with an annular phase plate in the emission path to create a spectral self-interference signal that is phase-dependent on the axial position of a fluorescent sample...
March 20, 2017: Optics Express
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