keyword
MENU ▼
Read by QxMD icon Read
search

Autofluorescence

keyword
https://www.readbyqxmd.com/read/29150629/statistically-strong-label-free-quantitative-identification-of-native-fluorophores-in-a-biological-sample
#1
Saabah B Mahbub, Martin Plöschner, Martin E Gosnell, Ayad G Anwer, Ewa M Goldys
Bioimaging using endogenous cell fluorescence, without any external biomarkers makes it possible to explore cells and tissues in their original native state, also in vivo. In order to be informative, this label-free method requires careful multispectral or hyperspectral recording of autofluorescence images followed by unsupervised extraction (unmixing) of biochemical signatures. The unmixing is difficult due to the scarcity of biochemically pure regions in cells and also because autofluorescence is weak compared with signals from labelled cells, typically leading to low signal to noise ratio...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150217/idiopathic-macular-telangiectasia-type-2-prevalence-and-a-morphometric-and-phenotypic-study
#2
R M Bayón-Porras, I Pascual-Camps, C Plaza-Laguardia, R Gallego-Pinazo
OBJECTIVE: Type 2 idiopathic macular telangiectasia (MacTel2) is a rare retinal disease that has still not been well-defined. The aim of the present manuscript is to describe the clinical features by multimodal retinal imaging, to present the functional characteristics, and to estimate the prevalence of the disease. METHODS: A retrospective study was conducted on the 12 eyes of 6 patients with MacTel2. Fundus colour photographs, fundus autofluorescence, fluorescein angiography, optical coherence tomography (OCT), and OCT-angiography were performed and subsequently analysed...
November 14, 2017: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/29145636/localization-and-functional-characterization-of-the-p-asn965ser-n965s-abca4-variant-in-mice-reveal-pathogenic-mechanisms-underlying-stargardt-macular-degeneration
#3
Laurie L Molday, Daniel Wahl, Marinko Sarunic, Robert S Molday
ABCA4 is a member of the superfamily of ATP-binding cassette (ABC) proteins that transports N-retinylidene-phosphatidylethanolamine (N-Ret-PE) across outer segment disc membranes thereby facilitating the removal of potentially toxic retinoid compounds from photoreceptor cells. Mutations in the gene encoding ABCA4 are responsible for Stargardt disease (STGD1), an autosomal recessive retinal degenerative disease that causes severe vision loss. To define the molecular basis for STGD1 associated with the p.Asn965Ser (N965S) mutation in the Walker A motif of nucleotide binding domain 1 (NBD1), we generated a p...
November 14, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29142843/intraoperative-real-time-localization-of-parathyroid-gland-with-near-infrared-fluorescence-imaging
#4
REVIEW
Sung Won Kim, Hyoung Shin Lee, Kang Dae Lee
Surgeons have cited difficulties in identifying the parathyroid glands (PG) during thyroidectomy. To overcome the limitation of naked eye, many studies on near-infrared fluorescence imaging of PGs have been introduced and suggested that fluorescence imaging is useful for both localizing PGs and evaluating their function. This imaging technique has been reported in two ways: (I) imaging using a fluorescent material called indocyanine green (ICG); and (II) autofluorescence using intrinsic fluorophores. These innovative and novel techniques are expected to have a significant impact on performing thyroid or parathyroid surgery...
October 2017: Gland Surgery
https://www.readbyqxmd.com/read/29142266/hyperspectral-raman-imaging-of-neuritic-plaques-and-neurofibrillary-tangles-in-brain-tissue-from-alzheimer-s-disease-patients
#5
Ralph Michael, Aufried Lenferink, Gijs F J M Vrensen, Ellen Gelpi, Rafael I Barraquer, Cees Otto
Neuritic plaques and neurofibrillary tangles are crucial morphological criteria for the definite diagnosis of Alzheimer's disease. We evaluated 12 unstained frontal cortex and hippocampus samples from 3 brain donors with Alzheimer's disease and 1 control with hyperspectral Raman microscopy on samples of 30 × 30 µm. Data matrices of 64 × 64 pixels were used to quantify different tissue components including proteins, lipids, water and beta-sheets for imaging at 0.47 µm spatial resolution. Hierarchical cluster analysis was performed to visualize regions with high Raman spectral similarities...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29141877/giving-invertebrates-an-eye-exam-an-ophthalmoscope-that-utilizes-the-autofluorescence-of-photoreceptors
#6
Annette Stowasser, Madeline Owens, Elke K Buschbeck
One of the most important functional features of eyes is focusing light, as both nearsightedness and farsightedness have major functional implications. Accordingly, refractive errors are frequently assessed in vertebrates, but not in the very small invertebrate eyes. We describe a micro-ophthalmoscope that takes advantage of autofluorescent properties of invertebrate photoreceptors and test the device on the relatively well-understood eyes of jumping spiders and flies. In each case, our measurements confirmed previous findings with a greater degree of accuracy...
November 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29136891/detection-and-quantification-of-extra-virgin-olive-oil-adulteration-by-means-of-autofluorescence-excitation-emission-profiles-combined-with-multi-way-classification
#7
Isabel Durán Merás, Jaime Domínguez Manzano, Diego Airado Rodríguez, Arsenio Muñoz de la Peña
Within olive oils, extra virgin olive oil is the highest quality and, in consequence, the most expensive one. Because of that, it is common that some merchants attempt to take economic advantage by mixing it up with other less expensive oils, like olive oil or olive pomace oil. In consequence, the characterization and authentication of extra virgin olive oils is a subject of great interest, both for industry and consumers. This paper reports the potential of front-face total fluorescence spectroscopy combined with second-order chemometric methods for the detection of extra virgin olive oils adulteration with other olive oils...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29135799/peripapillary-pachychoroid-syndrome
#8
Nopasak Phasukkijwatana, K Bailey Freund, Rosa Dolz-Marco, Mayss Al-Sheikh, Pearse A Keane, Catherine A Egan, Sandeep Randhawa, Jay M Stewart, Qingyun Liu, Alex P Hunyor, Allan Kreiger, Aaron Nagiel, Robert Lalane, Mansour Rahimi, Won Ki Lee, Lee Merrill Jampol, David Sarraf
PURPOSE: To describe the features of peripapillary pachychoroid syndrome (PPS), a novel pachychoroid disease spectrum (PDS) entity. METHODS: Medical records of 31 eyes (16 patients) with choroidal thickening associated with intraretinal and/or subretinal fluid in the nasal macula extending from the disk were reviewed (patients with PPS). Choroidal thickness was compared with 2 age-matched cohorts: typical PDS (17 eyes with central serous chorioretinopathy or pachychoroid neovasculopathy) and 19 normal eyes...
November 10, 2017: Retina
https://www.readbyqxmd.com/read/29135703/a-case-of-relapsing-retinal-pigment-epithelial-detachment-in-peripapillary-pachychoroid-pigment-epitheliopathy
#9
Fuyan Zhang, Yuxing Qiu, Jay M Stewart
PURPOSE: To report the findings in a patient with peripapillary pachychoroid pigment epitheliopathy who demonstrated a retinal pigment epithelial detachment (PED) that was present at some times but not other times. METHODS: A review of 24 months of the clinical course and findings on multimodal imaging, including color fundus photography, fundus autofluorescence, spectral domain optical coherence tomography, and enhanced depth imaging optical coherence tomography...
November 10, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/29135295/-re-engineering-of-glucose-exposure-in-peritoneal-dialysis
#10
Emília Mácsai, István Kiss
The significance of peritoneal dialysis in kidney replacement therapy is expected to increase, so it is important to reconsider glucose exposure to minimize the adverse effects. The first step was to develop biocompatible modern PD solutions to reduce the local and systemic adverse effects of current conventional glucose-based ones. According to the limited clinical experience, there are no clear data on better clinical outcome. Besides this there is a suspected theoretical correlation between development of encapsulating peritoneal sclerosis and chronic local irritation of peritoneal surface by glucose...
October 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29133672/spontaneous-anatomical-and-functional-recovery-of-bilateral-electric-shock-maculopathy
#11
Ratnesh Ranjan, George Joseph Manayath, Palmeera Dsouza, Venkatapathy Narendran
A 12-year-old boy presented with best-corrected visual acuity (BCVA) of 6/9 in both eyes following an episode of electric shock. Optical coherence tomography (OCT) showed disruption of the ellipsoid zone as well as retinal pigment epithelium (RPE) layer. Fundus autofluorescence (FAF) showed increased central hypoautofluorescence in both eyes. At 3-month follow-up, BCVA improved to 6/6 with OCT showing spontaneous resolution of maculopathy in both eyes with reorganized RPE layer and ellipsoid zone. To the best of our knowledge, this is the first case of bilateral electric shock maculopathy (ESM) with spontaneous anatomical as well as functional recovery...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29128078/applications-of-mass-cytometry-in-clinical-medicine-the-promise-and-perils-of-clinical-cytof
#12
REVIEW
Gregory K Behbehani
Mass cytometry is a novel technology similar to flow cytometry in which antibodies are tagged with heavy metal molecules rather than fluorophores and then detected with time-of-flight mass spectrometry. This enables measurement of up to 50 simultaneous parameters with no autofluorescent background and little or no spillover or required compensation. Mass cytometry has tremendous potential for the analysis of highly complex clinical samples for the diagnosis and monitoring of malignant and autoimmune disorders...
December 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/29127572/strong-surface-enhanced-florescence-of-carbon-dot-labeled-bacteria-cells-observed-with-high-contrast-on-gold-film
#13
Rostislav Bukasov, Olena Filchakova, Kristina Gudun, Mohamed Bouhrara
Strong surface (metal) enhanced fluorescence (SEF or MEF) is observed from clusters and single E coli bacteria cells labeled with Carbon nanodots (CDs), which were synthesized from date pits. The enhancement factor (EF) for SEF of the cell clusters were close to 50 for both 533 and 633 nm laser excitation wavelength. Those EFs are ratios of emission peak areas from CD labeled cell clusters on gold film to the peak areas of the same batch cell clusters on glass substrate. SEF with 633 nm excitation performed better than SEF with 532 nm excitation, achieving higher fluorescence intensity and much higher contrast...
November 10, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/29126757/quantifying-the-rate-of-ellipsoid-zone-loss-in-stargardt-disease
#14
Cindy X Cai, Jacob G Light, James T Handa
PURPOSE: To determine a reliable method of using the ellipsoid zone (EZ) on optical coherence tomography (OCT) to track disease progression in Stardgardt disease (STGD). DESIGN: retrospective reliability study METHODS: STGD patients with genetically confirmed ABCA4 gene mutations seen at the Wilmer Eye Institute with follow-up visits separated by at least 12 months were identified. Spectral domain optical coherence tomography (SD-OCT) macula volume scans centered at the fovea and fundus autofluorescence (FAF) images were obtained...
November 7, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29125138/detection-accuracy-for-epithelial-dysplasia-using-an-objective-autofluorescence-visualization-method-based-on-the-luminance-ratio
#15
Nanami Yamamoto, Koji Kawaguchi, Hisako Fujihara, Mitsuhiko Hasebe, Yuta Kishi, Masaaki Yasukawa, Kenichi Kumagai, Yoshiki Hamada
The autofluorescence visualization method (AVM) uses blue excitation light to assist in the diagnosis of epithelial dysplasia. It detects epithelial dysplasia as a black area, which is known as fluorescence visualization loss (FVL). In this study, we evaluated the detection accuracy for epithelial dysplasia of the tongue using the objective AVM and assessed its possible clinical utility. Seventy-nine tongue specimens clinically suspected to have leukoplakia or squamous cell carcinoma (SCC) were analyzed. First, the AVM was subjectively performed using the Visually Enhanced Lesion scope (VELscope), and the iodine-staining method was then performed...
November 10, 2017: International Journal of Oral Science
https://www.readbyqxmd.com/read/29122718/near-infrared-autofluorescence-image-guided-parathyroid-gland-mapping-in-thyroidectomy
#16
Sung Won Kim, Hyoung Shin Lee, Yeh-Chan Ahn, Chan Woo Park, Seok Won Jeon, Chang Hoi Kim, Jae Beom Ko, Chulho Oak, Yikeun Kim, Kang Dae Lee
BACKGROUND: Studies to date have shown that near-infrared autofluorescence imaging (NIR) can detect the parathyroid gland during thyroidectomy. However, there are no reports that NIR imaging can identify the parathyroid gland when covered with fibrofatty tissue before identification by a surgeon's naked eye. In this study, we investigated the feasibility of parathyroid gland mapping to facilitate early identification of the parathyroid gland during thyroidectomy. STUDY DESIGN: Seventy parathyroid glands from 38 patients who underwent thyroidectomy for papillary thyroid cancer were included in this prospective study...
November 6, 2017: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/29122325/impact-of-autofluorescence-based-identification-of-parathyroids-during-total-thyroidectomy-on-postoperative-hypocalcemia-a-before-and-after-controlled-study
#17
Fares Benmiloud, Stanislas Rebaudet, Arthur Varoquaux, Guillaume Penaranda, Marie Bannier, Anne Denizot
BACKGROUND: The clinical impact of intraoperative autofluorescence-based identification of parathyroids using a near-infrared camera remains unknown. METHODS: In a before and after controlled study, we compared all patients who underwent total thyroidectomy by the same surgeon during Period 1 (January 2015 to January 2016) without near-infrared (near-infrared- group) and those operated on during Period 2 (February 2016 to September 2016) using a near-infrared camera (near-infrared+ group)...
November 6, 2017: Surgery
https://www.readbyqxmd.com/read/29121364/swept-source-optical-coherence-tomography-angiography-findings-in-torpedo-maculopathy
#18
Zaria Ali, Carol Lally Shields, Kirti Jasani, Tariq Mehmood Aslam, Konstantinos Balaskas
Torpedo maculopathy is a rare congenital anomaly of the retinal pigment epithelium (RPE). Various imaging modalities have been used to assess cases of torpedo maculopathy, including optical coherence tomography (OCT) and fundus autofluorescence (FAF). OCT angiography (OCTA) offers combined structural and flow characteristics of imaged lesions. The authors present OCTA findings in two cases of torpedo maculopathy. Common features include loss of RPE and choriocapillaris allowing greater visualization of larger vessels in the outer choroid...
November 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/29119058/next-generation-in-vivo-optical-imaging-with-short-wave-infrared-quantum-dots
#19
Oliver T Bruns, Thomas S Bischof, Daniel K Harris, Daniel Franke, Yanxiang Shi, Lars Riedemann, Alexander Bartelt, Frank B Jaworski, Jessica A Carr, Christopher J Rowlands, Mark W B Wilson, Ou Chen, He Wei, Gyu Weon Hwang, Daniel M Montana, Igor Coropceanu, Odin B Achorn, Jonas Kloepper, Joerg Heeren, Peter T C So, Dai Fukumura, Klavs F Jensen, Rakesh K Jain, Moungi G Bawendi
For in vivo imaging, the short-wavelength infrared region (SWIR; 1000-2000 nm) provides several advantages over the visible and near-infrared regions: general lack of autofluorescence, low light absorption by blood and tissue, and reduced scattering. However, the lack of versatile and functional SWIR emitters has prevented the general adoption of SWIR imaging by the biomedical research community. Here, we introduce a class of high-quality SWIR-emissive indium-arsenide-based quantum dots (QDs) that are readily modifiable for various functional imaging applications, and that exhibit narrow and size-tunable emission and a dramatically higher emission quantum yield than previously described SWIR probes...
2017: Nature biomedical engineering
https://www.readbyqxmd.com/read/29116964/an-elderly-patient-with-acute-transient-outer-retinal-disruption-resembling-bilateral-multiple-evanescent-white-dot-syndrome
#20
Cyrus Golshani, Orly Gal-Or, Vincent Giovinazzo, K Bailey Freund
PURPOSE: To report an unusual case of an elderly patient with transient outer retinal disruption resembling bilateral multiple evanescent white dot syndrome. METHODS: Observational case report. Fundus photographs, fluorescein angiography, standard and ultra-widefield fundus autofluorescence, and cross-sectional and en face optical coherence tomography were used to characterize and describe the clinical findings. RESULTS: A 67-year-old woman presented with decreased vision and floaters in her left eye...
November 7, 2017: Retinal Cases & Brief Reports
keyword
keyword
42539
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"