keyword
MENU ▼
Read by QxMD icon Read
search

Autofluorescence

keyword
https://www.readbyqxmd.com/read/28214549/atp-dependent-activity-and-mitochondrial-localization-of-drug-efflux-pumps-in-doxorubicin-resistant-breast-cancer-cells
#1
Julie Dartier, Elsa Lemaitre, Igor Chourpa, Caroline Goupille, Stéphane Servais, Stéphan Chevalier, Karine Mahéo, Jean-François Dumas
BACKGROUND: We hypothesized that, among the mechanisms of drug-resistance acquired by doxorubicin (DOX)-resistant breast cancer cells to maintain cell survival, ATP-dependent drug efflux pumps could be expressed in their mitochondrial membranes and this might limit the accumulation of DOX in this subcellular compartment in relation to mitochondrial ATP production. Methods/results Mitochondrial DOX accumulation: the presence and the activity of mitochondrial efflux pumps and their relationship with mitochondrial ATP synthesis were analyzed in DOX-resistant (MCF-7dox(R)) and -sensitive (MCF-7(S)) breast cancer cells...
February 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28213277/fluorescence-and-computed-tomography-for-assessing-the-biodistribution-of-sirna-after-intratracheal-application-in-mice
#2
Antonia Geyer, Cornelia Lorenzer, Sebastian Gehrig, Manuela Simlinger, Johannes Winkler, Haider Sami, Manfred Ogris
Pulmonary delivery of nucleic acids opens the possibility for direct treatment of lung diseases, like fibrosis, cancer, and infections. Lung retention and biodistribution of nucleic acids remain important issues for the development of suitable therapeutic approaches. Moreover, monitoring the dynamic biodistribution processes of siRNA after aerosol delivery can help in identifying bottlenecks and optimising therapeutic concepts. We investigated dynamic biodistribution events after intratracheal application of chemically stabilised siRNA labeled with near infrared emitting dye AlexaFluor750 (AF750)...
February 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28211922/applying-phasor-approach-analysis-of-multiphoton-flim-measurements-to-probe-the-metabolic-activity-of-three-dimensional-in-vitro-cell-culture-models
#3
Pirmin H Lakner, Michael G Monaghan, Yvonne Möller, Monilola A Olayioye, Katja Schenke-Layland
Fluorescence lifetime imaging microscopy (FLIM) can measure and discriminate endogenous fluorophores present in biological samples. This study seeks to identify FLIM as a suitable method to non-invasively detect a shift in cellular metabolic activity towards glycolysis or oxidative phosphorylation in 3D Caco-2 models of colorectal carcinoma. These models were treated with potassium cyanide or hydrogen peroxide as controls, and epidermal growth factor (EGF) as a physiologically-relevant influencer of cell metabolic behaviour...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205679/combined-raman-and-autofluorescence-ex-vivo-diagnostics-of-skin-cancer-in-near-infrared-and-visible-regions
#4
Ivan A Bratchenko, Dmitry N Artemyev, Oleg O Myakinin, Yulia A Khristoforova, Alexander A Moryatov, Sergey V Kozlov, Valery P Zakharov
No abstract text is available yet for this article.
February 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28205521/genetically-encoded-bioluminescent-voltage-indicator-for-multi-purpose-use-in-wide-range-of-bioimaging
#5
Shigenori Inagaki, Hidekazu Tsutsui, Kazushi Suzuki, Masakazu Agetsuma, Yoshiyuki Arai, Yuka Jinno, Guirong Bai, Matthew J Daniels, Yasushi Okamura, Tomoki Matsuda, Takeharu Nagai
We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluorescence from environment severely interferes fluorescence imaging...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205245/the-use-of-near-infrared-fluorescence-imaging-in-endocrine-surgical-procedures
#6
Bora Kahramangil, Eren Berber
Near-infrared fluorescence imaging in endocrine surgery is a new, yet highly investigated area. It involves indocyanine green use as well as parathyroid autofluorescence. Several groups have described their technique and reported on the observed utility. However, there is no consensus on technical details. Furthermore, the correlation between intraoperative findings and postoperative outcomes is unclear. With this study, we aim to review the current literature on fluorescence imaging and share our insights on technical details...
February 15, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28202480/multimodal-imaging-findings-in-hyper-early-stage-mewds
#7
Armelle Cahuzac, Benjamin Wolff, Thibaud Mathis, Marie-Hélène Errera, José-Alain Sahel, Martine Mauget-Faÿsse
OBJECTIVE: To describe a new stage of multiple evanescent white dot syndrome (MEWDS), occurring at a very early phase of the disease. METHODS: Retrospective analysis of clinical, angiographic and tomographic findings in four patients with 'hyper-early' stage MEWDS. RESULTS: In four patients seen within 1 week of the onset of symptoms, fundus analysis revealed macular granity and the classic yellow-white dots, some having no corresponding hyperautofluorescent pattern...
February 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28199885/evaluation-of-polyesteramide-pea-and-polyester-plga-microspheres-as-intravitreal-drug-delivery-systems-in-albino-rats
#8
Tobias Peters, Seong-Woo Kim, Vinicius Castro, Krunoslav Stingl, Torsten Strasser, Sylvia Bolz, Ulrich Schraermeyer, George Mihov, MengMeng Zong, Vanessa Andres-Guerrero, Rocio Herrero Vanrell, Aylvin A Dias, Neil R Cameron, Eberhart Zrenner
PURPOSE: To study the suitability of injectable microspheres based on poly(ester amide) (PEA) or poly lactic-co-glycolic acid (PLGA) as potential vehicles for intravitreal drug delivery in rat eyes. Dexamethasone-loaded PEA microspheres (PEA + DEX) were also evaluated. METHODS: Forty male Sprague Dawley rats were divided into four groups that received different intravitreally injected microspheres: PEA group (n = 12); PLGA group (n = 12); PEA + DEX group (n = 8); and control group (no injection, n = 8)...
February 7, 2017: Biomaterials
https://www.readbyqxmd.com/read/28196414/polyelectrolyte-fiber-assembly-of-plant-derived-spider-silk-like-proteins
#9
Congyue Annie Peng, Julia A Russo, Todd A Lyda, William Richard Marcotte
Spider dragline silk is a proteinaceous material that combines superior toughness and biocompatibility, a promising bio-material. The distinct protein structure and the fiber formation process contribute to the superior toughness of dragline silk. Previously, we have produced recombinant spider silk-like proteins in transgenic tobacco that are readily purified from plant extracts. The plant-derived spidroin-like proteins consisted of native major ampullate spidroin 1 or spidroin 2 N- and C-termini flanking 8, 16, or 32 copies of their respective consensus block repeats (mini-spidroins)...
February 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28195603/classification-of-exudative-age-related-macular-degeneration-with-pachyvessels-on-en-face-swept-source-optical-coherence-tomography
#10
Danny Siu-Chun Ng, Malini Bakthavatsalam, Frank Hiu-Ping Lai, Carol Yim-Lui Cheung, Gemmy Chu-Ming Cheung, Fang Yao Tang, Chi Wai Tsang, Timothy Yuk-Yau Lai, Tien Yin Wong, Mårten Erik Brelén
Purpose: The purpose of this study was to classify exudative maculopathy by the presence of pachyvessels on en face swept-source optical coherence tomography (SSOCT). Methods: Consecutive patients with signs of exudative maculopathy underwent SSOCT, fluorescein and indocyanine green angiography (ICGA), ultra-widefield fundus color photography, and autofluorescence examinations. Images were analyzed in a masked fashion by two sets of four examiners in different sessions: (1) the presence of pachyvessels in en face OCT and (2) features of exudative maculopathy in conventional imaging modalities...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28192305/an-optical-method-for-reducing-green-fluorescence-from-urine-during-fluorescence-guided-cystoscopy
#11
Lars R Lindvold, Gregers G Hermann
Photodynamic diagnosis (PDD) of bladder tumour tissue significantly improves endoscopic diagnosis and treatment of bladder cancer in rigid cystoscopes in the operating theatre and thus reduces tumour recurrence. PDD comprises the use of blue light, which unfortunately excites green fluorescence from urine. As this green fluorescence confounds the desired red fluorescence of the PDD, methods for avoiding this situation particularly in cystoscopy using flexible cystoscopes are desirable. In this paper we demonstrate how a tailor made high power LED light source at 525 nm can be used for fluorescence assisted tumour detection using both a flexible and rigid cystoscope used in the outpatient department (OPD) and operating room (OR) respectively...
November 14, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28192298/near-infrared-808-and-980%C3%A2-nm-excited-photoluminescence-study-in-nd-doped-y2o3-phosphor-for-bio-imaging
#12
Prasenjit Prasad Sukul, Kaushal Kumar
The upconversion (UC) process in lanthanide-doped nanophosphors has attracted great research interest for its extensive application potential in biological in vitro and in vivo imaging due to the high tissue penetration depth of near-infrared excitation and low autofluorescence background. In this article, the authors report the synthesis of oxide nanophosphor of size  ⩽50 nm, which forms stable aqueous dispersion. The photoluminescence study is made on the nanophosphor upon 808 and 980 nm diode laser excitations...
October 11, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28187978/optical-coherence-tomography-examination-of-the-retinal-pigment-epithelium-in-best-vitelliform-macular-dystrophy
#13
Cynthia X Qian, Dionisio Charran, Cameron R Strong, Timothy J Steffens, Thiran Jayasundera, John R Heckenlively
PURPOSE: To describe the anatomic changes and natural history of vitelliform lesions in Best vitelliform macular dystrophy (BVMD) using spectral-domain optical coherence tomography (OCT). DESIGN: Prospective comparative case series. PARTICIPANTS: Twenty patients (40 eyes) with molecular confirmation of mutation in the BEST1 gene and 20 age-matched controls were included. METHODS: Color fundus photographs, fundus autofluorescence, and spectral-domain OCT were obtained, and these findings were compared between the 2 groups...
February 7, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28186509/hydroxychloroquine-retinopathy-an-emerging-problem
#14
M Latasiewicz, H Gourier, I H Yusuf, R Luqmani, S M Sharma, S M Downes
PurposeThe aim of this case series is to raise awareness of the emerging issue of serious retinal damage caused by the prolonged use of hydroxychloroquine (HCQ) and the importance of adequate and appropriate monitoring of visual function during treatment.Patient and methodsThis is a small retrospective case series of 3 patients on long-term HCQ who developed serious symptomatic retinal toxicity confirmed on imaging and functional testing.ResultsAll 3 patients were treated with HCQ for over 15 years; two for rheumatoid arthritis (RA), and the third for systemic lupus erythematosus (SLE)...
February 10, 2017: Eye
https://www.readbyqxmd.com/read/28179979/flexible-gastro-intestinal-endoscopy-clinical-challenges-and-technical-achievements
#15
REVIEW
Niehls Kurniawan, Martin Keuchel
Flexible gastro-intestinal (GI) endoscopy is an integral diagnostic and therapeutic tool in clinical gastroenterology. High quality standards for safety, patients' comfort, and efficiency have already been achieved. Clinical challenges and technical approaches are discussed in this short review. Image enhanced endoscopy for further characterization of mucosal and vascular patterns includes dye-spray or virtual chromoendoscopy. For confocal laser endoscopy, endocytoscopy, and autofluorescence clinical value has not yet been finally evaluated...
2017: Computational and Structural Biotechnology Journal
https://www.readbyqxmd.com/read/28170224/sub-10-nm-sr2luf7-yb-er-sr2gdf7-srf2-up-conversion-nanocrystals-for-up-conversion-luminescence-magnetic-resonance-computed-tomography-trimodal-bioimaging
#16
Cailing Chen, Jianhua Liu, Ying Chen, Chunguang Li, Xiaomin Liu, He Huang, Chen Liang, Yue Lou, Zhan Shi, Shouhua Feng
Herein, sub-10 nm core-shell nanocrystals (NCs), which select Sr2LuF7:Yb/Er as core, Sr2GdF7 as middle shell, and SrF2 as an outermost shell, were synthesized by a seed-mediated growth process. The NCs possess good crystallinity, morphology, and up-conversion luminescent properties. After modification by polyethylenimine branched (PEI), in vitro cell up-conversion imaging with low autofluorescence was realized. Due to the presence of Gd(3+) ions, in vivo magnetic resonance (MR) imaging was also achieved with these designed NCs...
February 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28169309/correlative-imaging-of-the-murine-hind-limb-vasculature-and-muscle-tissue-by-microct-and-light-microscopy
#17
Laura Schaad, Ruslan Hlushchuk, Sébastien Barré, Roberto Gianni-Barrera, David Haberthür, Andrea Banfi, Valentin Djonov
A detailed vascular visualization and adequate quantification is essential for the proper assessment of novel angiomodulating strategies. Here, we introduce an ex vivo micro-computed tomography (microCT)-based imaging approach for the 3D visualization of the entire vasculature down to the capillary level and rapid estimation of the vascular volume and vessel size distribution. After perfusion with μAngiofil®, a novel polymerizing contrast agent, low- and high-resolution scans (voxel side length: 2.58-0.66 μm) of the entire vasculature were acquired...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28169259/fluorescence-of-candida-in-diagnosis-of-oral-candidiasis
#18
L R Kumaraswamy Naik, Pushparaja Shetty, M S Krishna Prasad, Vimal Kumar Karnaker, Sarosh E Shroff, Lal P Madathil
BACKGROUND: Many pathogenic fungi fluoresce in hematoxylin and eosin stained sections, and Papanicolaou (PAP)-stained smears under ultraviolet illumination. In theory, this phenomenon could aid in the diagnosis of common fungal infections without the delay which is usually associated with special stains. OBJECTIVE: To evaluate the role of fluorescence as a rapid screening technique for oral infections caused by Candida organisms in exfoliative smears of oral candidiasis...
November 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/28166161/characterizing-photoreceptor-changes-in-acute-posterior-multifocal-placoid-pigment-epitheliopathy-using-adaptive-optics
#19
Philipp K Roberts, Peter L Nesper, Alex C Onishi, Dimitra Skondra, Lee M Jampol, Amani A Fawzi
PURPOSE: To characterize lesions of acute posterior multifocal placoid pigment epitheliopathy (APMPPE) by multimodal imaging including adaptive optics scanning laser ophthalmoscopy (AOSLO). METHODS: We included patients with APMPPE at different stages of evolution of the placoid lesions. Color fundus photography, spectral domain optical coherence tomography, infrared reflectance, fundus autofluorescence, and AOSLO images were obtained and registered to correlate microstructural changes...
February 3, 2017: Retina
https://www.readbyqxmd.com/read/28161925/the-use-of-microperimetry-to-detect-functional-progression-in-non-neovascular-age-related-macular-degeneration-a-systematic-review
#20
REVIEW
Evan N Wong, Avenell L Chew, William H Morgan, Praveen J Patel, Fred K Chen
We reviewed the current literature on the ability of microperimetry to detect non-neovascular age-related macular degeneration (AMD) disease progression. The index test was retinal sensitivity measurement assessed by microperimetry and comparators were other functional measures (best-corrected and low-luminance visual acuities, and fixation stability) and structural parameters [retinal thickness, choroidal thickness, and area of geographic atrophy (GA) determined by color fundus photographs, short-wave or near-infrared fundus autofluorescence]...
January 2017: Asia-Pacific Journal of Ophthalmology
keyword
keyword
42539
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"