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https://www.readbyqxmd.com/read/29761411/detection-of-pathogenic-viruses-in-the-ambient-air-in-seoul-korea
#1
Tae-Hee Han, Sang-Hun Park, Ju-Young Chung, Hyo-Won Jeong, Jihun Jung, Jae-In Lee, Young-Ok Hwang, Il-Young Kim, Jip-Ho Lee, Kweon Jung
The possible transport of pathogenic microorganisms during Asian dust events could be an important concern for health workers; however, this is still uncertain owing to a lack of supporting evidence. The present study aimed to investigate the presence of pathogenic microorganisms in air samples collected during the Asian and non-Asian dust periods. Between March and September 2016, air samples were collected at three weather observation stations in Seoul using a high-volume air sampler. Multiplex PCR was performed using the Allplex™ respiratory and gastrointestinal panel assay kits to detect 46 microorganisms...
May 14, 2018: Food and Environmental Virology
https://www.readbyqxmd.com/read/29755444/microfluidic-pcr-amplification-and-miseq-amplicon-sequencing-techniques-for-high-throughput-detection-and-genotyping-of-human-pathogenic-rna-viruses-in-human-feces-sewage-and-oysters
#2
Mamoru Oshiki, Takayuki Miura, Shinobu Kazama, Takahiro Segawa, Satoshi Ishii, Masashi Hatamoto, Takashi Yamaguchi, Kengo Kubota, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Naohiro Kobayashi, Nobuo Araki, Daisuke Sano
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high-throughput detection-and-genotyping tool for 11 human RNA viruses [Aichi virus; astrovirus; enterovirus; norovirus genogroup I (GI), GII, and GIV; hepatitis A virus; hepatitis E virus; rotavirus; sapovirus; and human parechovirus] using a microfluidic device and next-generation sequencer...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29753903/detection-of-nineteen-enteric-viruses-in-raw-sewage-in-japan
#3
Aksara Thongprachum, Tsuguto Fujimoto, Sayaka Takanashi, Hiroyuki Saito, Shoko Okitsu, Hiroyuki Shimizu, Pattara Khamrin, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima
One-year surveillance for enteric viruses in raw sewage was conducted in Kansai area, central part of Japan from July 2015 to June 2016. The raw sewage was collected monthly from an inlet polluted pool and was concentrated by polyethylene glycol (PEG) precipitation. Twelve sewage samples were screened for nineteen kinds of enteric viruses by using RT-PCR method and further analyzed by nucleotide sequencing. Twelve enteric viruses were found in the investigative sewage samples. Rotavirus A and norovirus GI and GII with several genotypes were detected all year round...
May 10, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29746455/enterovirus-and-parechovirus-surveillance-united-states-2014-2016
#4
Glen R Abedi, John T Watson, W Allan Nix, M Steven Oberste, Susan I Gerber
Infections caused by enteroviruses (EV) and parechoviruses (PeV), members of the Picornaviridae family, are associated with various clinical manifestations, including hand, foot, and mouth disease; respiratory illness; myocarditis; meningitis; and sepsis; and can result in death. The genus Enterovirus includes four species of enterovirus (A-D) known to infect humans, and the genus Parechovirus includes one species (A) that infects humans. These species are further divided into types, some of which are associated with specific clinical manifestations...
May 11, 2018: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29738080/molecular-detection-and-phylogenetic-analysis-of-human-parechovirus-in-individuals-with-acute-diarrhea-and-healthy-controls-in-guangzhou-china
#5
Xuejiao Chen, Tingli Shi, Jianhua Huang, Gang Xiao, Jing Huang, Yiquan Xiong, Xiufen Li, Huifang Chen, Xueyan Zheng, Shouyi Yu, Qing Chen
Human parechoviruses (HPeVs) are prevalent in young children; however, their effects are incompletely understood. We investigated the prevalence, genotype distribution, and phylogeny of HPeVs in individuals with diarrhea (n=430) and healthy controls (n=93), by analysis of stool specimens collected from July 2013 to December 2014; 51 (11.86%) and 6 (6.45%) specimens were HPeV-positive, respectively. HPeV1A occurred in 28 (6.51%) and 6 (6.45%) individuals with diarrhea and controls, respectively, whereas HPeV1B (3...
May 8, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29720076/paediatric-active-enhanced-disease-surveillance-paeds-annual-report-2015-prospective-hospital-based-surveillance-for-serious-paediatric-conditions
#6
Jocelynne E McRae, Helen E Quinn, Kristine Macartney
Introduction: The Paediatric Active Enhanced Disease Surveillance (PAEDS) network is a hospital-based active surveillance system employing prospective case ascertainment for selected serious childhood conditions, particularly vaccine preventable diseases and potential adverse events following immunisation (AEFI). PAEDS data is used to better understand these conditions, inform policy and practice under the National Immunisation Program, and enable rapid public health responses for certain conditions of public health importance...
September 1, 2017: Communicable Diseases Intelligence Quarterly Report
https://www.readbyqxmd.com/read/29716506/parechovirus-an-important-emerging-infection-in-young-infants
#7
Philip N Britton, Cheryl A Jones, Kristine Macartney, Allen C Cheng
Epidemics of human parechovirus (HPeV) causing disease in young children have occurred every 2 years in Australia since 2013. HPeV genotype 3 caused the epidemic from late 2017 to early 2018. Most HPeV infections cause no or mild symptoms including gastroenteritis or influenza-like illness. Characteristically, young infants present with fever, irritability and on occasions a diffuse rash ("red, hot and angry" babies). Severe disease can manifest as meningoencephalitis, seizures or sepsis-like presentations (including septic shock), or less common presentations including signs of surgical abdomen...
May 7, 2018: Medical Journal of Australia
https://www.readbyqxmd.com/read/29709990/human-parechovirus-infection-in-children-in-taiwan-a-retrospective-single-hospital-study
#8
Jenn-Tzong Chang, Yao-Shen Chen, Bao-Chen Chen, Tsi-Shu Huang, Tsung-Hsien Chang
To understand human parechovirus (HPeV) infections in Taiwanese children, we analyzed data for 112 children (age ≤ 10 years) with HPeV infection diagnosed from July 2007 to June 2016 in a medical centre in Kaohsiung, southern Taiwan. Infection was with HPeV1 (n=94), HPeV3 (n=3), HPeV4 (n=3), HPeV6 (n=1) and non-typeable HPeV (n=11). We compared the clinical implication for children younger than 3 months (n=56) and 3 months and older (n=31), excluding the 25 children with concomitant infection. Fever was noted in almost half of the children younger than 3 months but was more frequent in older than younger children (83...
April 27, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29709970/analysis-of-hpev-genotype-in-yokohama-district-from-2000-2016
#9
Tomoko Soga Momoki
Human parechovirus (HPeV) infections in Yokohama City, Japan, were surveyed from 2000 to 2016. The sequence of the VP1 region of HPeV was used to construct a phylogenetic tree and to reveal the putative amino acid (aa) sequences. The phylogenetic analysis showed the presence of three genotypes in Yokohama City: HPeV1 (25 specimens), HPeV3 (86 specimens), and HPeV4 (2 specimens). HPeV1 was detected nearly every year, with the highest number detected in 2014. HPeV3 was not detected until 2005 but detected during a 3-year period thereafter...
April 27, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29643409/intrinsically-disordered-n-termini-in-human-parechovirus-1-capsid-proteins-bind-encapsidated-rna
#10
Shabih Shakeel, James D Evans, Mark Hazelbaker, C Cheng Kao, Robert C Vaughan, Sarah J Butcher
Human parechoviruses (HPeV) are picornaviruses with a highly-ordered RNA genome contained within icosahedrally-symmetric capsids. Ordered RNA structures have recently been shown to interact with capsid proteins VP1 and VP3 and facilitate virus assembly in HPeV1. Using an assay that combines reversible cross-linking, RNA affinity purification and peptide mass fingerprinting (RCAP), we mapped the RNA-interacting regions of the capsid proteins from the whole HPeV1 virion in solution. The intrinsically-disordered N-termini of capsid proteins VP1 and VP3, and unexpectedly, VP0, were identified to interact with RNA...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29601457/longitudinal-association-between-human-parechovirus-central-nervous-system-infection-and-gross-motor-neurodevelopment-in-young-children
#11
Ted M T van Hinsbergh, Roy G Elbers, Marceline A M van Furth, Charlie C C Obihara
BACKGROUND: A paucity of studies investigated the association between human parechovirus (HPeV) central nervous system (CNS) infection and motor and neurocognitive development of children. This study describes the gross-motor function (GMF) in young children during 24 months after HPeV-CNS-infection compared with children in whom no pathogen was detected. METHODS: GMF of children was assessed with alberta infant motor scale, bayley scales of infant and toddler development or movement assessment battery for children...
March 27, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29561512/human-parechovirus-meningitis-with-adverse-neurodevelopmental-outcome-a-case-report
#12
Mylene C Berk, Andrea Hl Bruning, Aleid G van Wassenaer-Leemhuis, Katja C Wolthers, Dasja Pajkrt
Human parechovirus (HPeV) infections usually cause mild symptoms in children. Although their contribution to severe disease in young children - such as neonatal sepsis and meningo-encephalitis - is increasingly recognized, data on long-term consequences are scarce. Here we present the case of a five-year old boy with severe long-term neurodevelopmental sequelae following HPeV-3 meningitis.
March 14, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29526382/neonatal-nonpolio-enterovirus-and-parechovirus-infections
#13
REVIEW
Nada Harik, Roberta L DeBiasi
Nonpolio enteroviruses and parechoviruses are frequent causes of neonatal infection. Clinical manifestations of infection range from asymptomatic infection to mild infection without sequelae to septic shock with muiltiorgan failure. Neonates with clinically apparent infection typically have mothers and/or other contacts with recent symptoms consistent with a viral illness. Severe neonatal infection with nonpolio enterovirus or parechovirus cannot be differentiated clinically from serious bacterial infection...
April 2018: Seminars in Perinatology
https://www.readbyqxmd.com/read/29502122/aichivirus-in-children-with-diarrhea-in-northern-italy
#14
Massimiliano Bergallo, Ilaria Galliano, Paola Montanari, Marco Rassu, Valentina Daprà
OBJECTIVE: Since its discovery, Aichivirus (AiV) A has been detected, with an incidence of 0.9-4.1%, primarily when studying outbreaks of diarrhea in children or young adults. In this paper, we report the first detection of AiV in Piedmont, Italy, in pediatric patients. METHODS: A total of 159 fecal specimens (from 96 males and 63 females) previously screened for rotaviruses, adenoviruses, noroviruses, human parechoviruses, saliviruses, and sapoviruses were collected from infants and children with acute gastroenteritis...
2017: Intervirology
https://www.readbyqxmd.com/read/29471623/monitoring-human-enteric-viruses-in-wastewater-and-relevance-to-infections-encountered-in-the-clinical-setting-a-one-year-experiment-in-central-france-2014-to-2015
#15
Maxime Bisseux, Jonathan Colombet, Audrey Mirand, Anne-Marie Roque-Afonso, Florence Abravanel, Jacques Izopet, Christine Archimbaud, Hélène Peigue-Lafeuille, Didier Debroas, Jean-Luc Bailly, Cécile Henquell
BackgroundHuman enteric viruses are resistant in the environment and transmitted via the faecal-oral route. Viral shedding in wastewater gives the opportunity to track emerging pathogens and study the epidemiology of enteric infectious diseases in the community. Aim: The aim of this study was to monitor the circulation of enteric viruses in the population of the Clermont-Ferrand area (France) by analysis of urban wastewaters. Methods: Raw and treated wastewaters were collected between October 2014 and October 2015 and concentrated by a two-step protocol using tangential flow ultrafiltration and polyethylene glycol precipitation...
February 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29438743/detection-and-molecular-characterization-of-enteric-viruses-in-children-with-acute-gastroenteritis-in-northern-italy
#16
Valeria Biscaro, Giorgio Piccinelli, Franco Gargiulo, Giovanni Ianiro, Arnaldo Caruso, Francesca Caccuri, Maria Antonia De Francesco
Enteric viral infections are a major concern for public health, and viral acute gastroenteritis is the principal cause of pediatric morbidity and mortality worldwide, mostly in developing countries. The purpose of this study was to determine the prevalence of different enteric viruses detected in a pediatric population with acute gastroenteritis symptoms, and to characterize the strains detected. Stools were collected from children, aged from 2 months to 15 years old, admitted to one of the main hospitals of Northern Italy, between November 2015 and October 2016...
June 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29406468/impact-of-cerebrospinal-fluid-multiplex-assay-on-diagnosis-and-outcomes-of-central-nervous-system-infections-in-children-a-before-and-after-cohort-study
#17
Matthew P O'Brien, Joshua R Francis, Ian M Marr, Robert W Baird
BACKGROUND: This study evaluated the performance of cerebrospinal fluid (CSF) multiplex assay in the diagnosis of pediatric CNS infection, and assessed for the effect on clinical management. STUDY DESIGN: A 15-month prospective cohort of pediatric patients with confirmed CNS infection was compared with a 15-month retrospective cohort from the Top End region of the Northern Territory, Australia. The study characterized all the CNS infections over the 30-month period and compared the time to organism identification and antibiotic management before and after the introduction of the multiplex assay...
February 5, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29402695/differential-removal-of-human-pathogenic-viruses-from-sewage-by-conventional-and-ozone-treatments
#18
Hao Wang, Per Sikora, Carolin Rutgersson, Magnus Lindh, Tomas Brodin, Berndt Björlenius, D G Joakim Larsson, Heléne Norder
Sewage contains a mixed ecosystem of diverse sets of microorganisms, including human pathogenic viruses. Little is known about how conventional as well as advanced treatments of sewage, such as ozonation, reduce the environmental spread of viruses. Analyses for viruses were therefore conducted for three weeks in influent, after conventional treatment, after additional ozonation, and after passing an open dam system at a full-scale treatment plant in Knivsta, Sweden. Viruses were concentrated by adsorption to a positively charged filter, from which they were eluted and pelleted by ultracentrifugation, with a recovery of about 10%...
April 2018: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29223126/hpev-3-predominated-among-parechovirus-a-positive-infants-during-an-outbreak-in-2013-2014-in-queensland-australia
#19
Donna McNeale, Claire Y T Wang, Katherine E Arden, Ian M Mackay
BACKGROUND: Parechoviruses (HPeV) are endemic seasonal pathogens detected from the respiratory tract, gut, blood and central nervous system (CNS) of children and adults, sometimes in conjunction with a range of acute illnesses. HPeV CNS infection may lead to neurodevelopmental sequelae, especially following infection by HPeV-3, hence screening and genotyping are important to inform epidemiology, aetiology and prognosis. OBJECTIVES: To identify and characterise HPeVs circulating during an outbreak between November 2013 and April 2014 in Queensland, Australia...
January 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29207707/diagnostic-approach-to-viral-acute-encephalitis-syndrome-aes-in-paediatric-age-group-a-study-from-new-delhi
#20
Shipra Goel, Anita Chakravarti, Mukta Mantan, Surinder Kumar, Md Anzar Ashraf
Introduction: Acute Encephalitis Syndrome has heralded the emergence of multiple virulent pathogens, which may result in severe morbidity and mortality. In India, encephalitis is not notified and there has been a dearth of analysis for trends in encephalitis death rates and causation. A downward trend has been observed in encephalitis deaths, due to 'known' causes, which can be largely explained by improvement in diagnostic, treatment, and prevention methods. There is still a very high proportion of encephalitis deaths in developing countries, where the aetiological diagnosis of the pathogen is not established and thus, lies the importance of monitoring encephalitis morbidity and mortality with a view to improve pathogen diagnosis and identify emerging infectious diseases...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
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