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Toxoplasmosis gondii

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https://www.readbyqxmd.com/read/28643940/proteomic-profiling-of-extracellular-vesicles-secreted-from-toxoplasma-gondii
#1
Pryscilla Fanini Wowk, Maria Luisa Zardo, Halisson Tesseroli Miot, Samuel Goldenberg, Paulo Costa Carvalho, Patricia Alves Mörking
Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of EVs derived from T. gondii on the immune response and its relevance in a physiological context is unknown. Here we disclose the first proteomic profiling of T. gondii EVs compared to EVs isolated from a human foreskin fibroblast infected cell line cultured in a vesicle- free medium...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28642841/strain-and-dose-dependent-reduction-of-toxoplasma-gondii-burden-in-pigs-is-associated-with-interferon-gamma-production-by-cd8-lymphocytes-in-a-heterologous-challenge-model
#2
Malgorzata Jennes, Stéphane De Craeye, Bert Devriendt, Katelijne Dierick, Pierre Dorny, Eric Cox
Toxoplasma gondii is a worldwide prevalent parasite of humans and animals. The global infection burden exceeds yearly one million disability-adjusted life years (DALY's) in infected individuals. Therefore, effective preventive measures should be taken to decrease the risk of infection in humans. Although human toxoplasmosis is predominantly foodborne by ingestion of tissue cysts in meat from domestic animals such as pigs, the incidence risk is difficult to estimate due to the lack of screening of animals for infection and insights in location and persistence of the parasite in the tissues...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28630781/development-of-neurological-mouse-model-for-toxoplasmosis-using-toxoplasma-gondii-isolated-from-chicken-in-kenya
#3
John Mokua Mose, David Muchina Kamau, John Maina Kagira, Naomi Maina, Maina Ngotho, Adele Njuguna, Simon Muturi Karanja
Animal models for the toxoplasmosis are scarce and have limitations. In this study, a neurological mouse model was developed in BALB/c mice infected intraperitoneally with 15 cysts of a Toxoplasma gondii isolate. The mice were monitored for 42 days and euthanized at different time points. Another group of mice were orally treated with dexamethasone (DXM: 2.66 mg/kg daily, 5.32 mg/kg daily) at 42 days after infection and monitored for a further 42 days. A mortality rate of 15% and 28.6% was observed in mice given 2...
2017: Pathology Research International
https://www.readbyqxmd.com/read/28630403/new-molecular-tools-in-neospora-caninum-for-studying-apicomplexan-parasite-proteins
#4
Caroline M Mota, Allan L Chen, Kevin Wang, Santhosh Nadipuram, Ajay A Vashisht, James A Wohlschlegel, Tiago W P Mineo, Peter J Bradley
The development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum Apicomplexa. We have established a system specifically designed for Neospora caninum, and used this system as a heterologous platform for the expression of foreign genes. Plasmid constructs containing fluorescent proteins or targeted genes of Toxoplasma gondii, driven by N. caninum promoters, have yielded robust expression and correct trafficking of target gene products as assessed by immunofluorescence assays and Western blot analyses...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28630065/ccr5-is-involved-in-interruption-of-pregnancy-in-mice-infected-with-toxoplasma-gondii-during-early-pregnancy
#5
Maki Nishimura, Kousuke Umeda, Masayuki Suwa, Hidefumi Furuoka, Yoshifumi Nishikawa
Toxoplasmosis can cause abortion in pregnant humans and other animals; however, the mechanism of abortion remains unknown. C-C chemokine receptor type 5 (CCR5) is essential for host defense in T. gondii infection. To investigate the relationship between CCR5 and abortion in toxoplasmosis, we inoculated wild-type and CCR5-deficient (CCR5(-/-)) mice with T. gondii tachyzoites intraperitoneally on day 3 of pregnancy (E3). The pregnancy rate decreased as pregnancy progressed in the infected wild-type mice. Histopathologically, no inflammatory lesions were observed in the fetoplacental tissues...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28629230/association-between-toxoplasma-gondii-types-and-outcomes-of-human-infection-a-meta-analysis
#6
Jing Xia, Xin-Yu Cheng, Xiao-Jun Wang, Hong-Juan Peng
The virulence and pathogenicity of various types of Toxoplasma gondii differ considerably in mice. Recent studies have claimed that similar phenomenon was observed in humans, but no relevant studies have been performed to validate this finding. In addition, reports showing association between a given T. gondii type and outcomes of human infection yielded conflicting results. To provide a more precise estimation of the association and a more reliable conclusion on this subject, we performed this meta-analysis...
June 20, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28629167/igg-avidity-test-in-congenital-toxoplasmosis-diagnoses-in-newborns
#7
Zulmirene Cardoso Fonseca, Isolina Maria Xavier Rodrigues, Natália Cruz E Melo, Juliana Boaventura Avelar, Ana Maria Castro, Mariza Martins Avelino
The goal of this study was to investigate the importance of IgG avidity testing in newborns (NBs) diagnosed with early congenital toxoplasmosis. We collected samples from 88 puerperae infected by Toxoplasma gondii (T. gondii) and their NBs (48 acutely-infected puerperae (AIP) and 40 chronically-infected puerperae (CIP)), from two public maternity hospitals in Goiania city, Goias, Brazil, from 2010 to 2015. Specific anti-T. gondii IgM and IgG serum levels and IgG avidity tests were evaluated using chemiluminescence...
June 18, 2017: Pathogens
https://www.readbyqxmd.com/read/28626452/proteomic-differences-between-developmental-stages-of-toxoplasma-gondii-revealed-by-itraq-based-quantitative-proteomics
#8
Ze-Xiang Wang, Chun-Xue Zhou, Hany M Elsheikha, Shuai He, Dong-Hui Zhou, Xing-Quan Zhu
Toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. Understanding proteomic variations across the life-cycle stages of T. gondii may improve the understanding of molecular adaption mechanism of T. gondii across life-cycle stages, and should have implications for the development of new treatment and prevention interventions against T. gondii infection. Here, we utilized LC-MS/MS coupled with iTRAQ labeling technology to identify differentially expressed proteins (DEPs) specific to tachyzoite (T), bradyzoites-containing cyst (C) and sporulated oocyst (O) stages of the cyst-forming T...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28625894/evaluation-of-protective-immunity-induced-by-dna-vaccination-with-genes-encoding-toxoplasma-gondii-gra17-and-gra23-against-acute-toxoplasmosis-in-mice
#9
Wei-Ning Zhu, Jin-Lei Wang, Kai Chen, Dong-Mei Yue, Xiao-Xuan Zhang, Si-Yang Huang, Xing-Quan Zhu
Toxoplasma gondii, an obligatory intracellular protozoan, can cause serious public health problems and economic losses worldwide. Two novel dense granule proteins (GRA17 and GRA23) were recently identified as T. gondii-secreted proteins which are localized to the parasitophorous vacuole membrane (PVM) and can mediate the movement of small molecules between the host cell and parasitophorous vacuole (PV). In the present study, we evaluated the protective immunity induced by DNA vaccination with genes encoding GRA17 and GRA23 against acute toxoplasmosis in mice...
June 15, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28615877/pcr-rflp-based-genotyping-of-indian-isolates-of-toxoplasma-gondii
#10
Hilal Ahmad Rather, Mir Mehraj Din, Aasif Ahmad Sheikh, Anup Kumar Tewari, Biswa Ranjan Maharana
Toxoplasma gondii is an apicomplexan parasite capable of infecting a wide variety of warm-blooded animals, including birds and humans and is zoonotically important too. Felidae serve its definitive hosts and most infections are inoccous while in various intermediate hosts (e.g. sheep), it is responsible for abortion, still births. Humans which are immune compromised are also susceptible to toxoplasmosis. Most of the epidemiological studies have revealed it to be belonging to three clonal types with exceptions in South Africa having atypical isolates...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28609444/identification-of-compounds-that-suppress-toxoplasma-gondii-tachyzoites-and-bradyzoites
#11
Yuho Murata, Tatsuki Sugi, Louis M Weiss, Kentaro Kato
Drug treatment for toxoplasmosis is problematic, because current drugs cannot eradicate latent infection with Toxoplasma gondii and can cause bone marrow toxicity. Because latent infection remains after treatment, relapse of infection is a problem in both infections in immunocompromised patients and in congenitally infected patients. To identify lead compounds for novel drugs against Toxoplasma gondii, we screened a chemical compound library for anti-Toxoplasma activity, host cell cytotoxicity, and effect on bradyzoites...
2017: PloS One
https://www.readbyqxmd.com/read/28606185/non-malarial-infectious-diseases-of-antenatal-care-in-pregnant-women-in-franceville-gabon
#12
Irene Pegha Moukandja, Edgard Brice Ngoungou, Guy Joseph Lemamy, Ulrick Bisvigou, Antoine Gessain, Fousseyni S Toure Ndouo, Mirdad Kazanji, Jean Bernard Lekana-Douki
BACKGROUND: In sub-tropical countries, infectious diseases remain one of the main causes of mortality. Because of their lack of active immunity, pregnant women and their unborn children represent the most susceptible people. In Gabon, data on infectious diseases of pregnant women such as syphilis and rubella are either scarce or very old. Few studies have assessed T. gondii infection during pregnancy in the country. Here, we evaluate seroprevalence of HIV, HTVL-1, syphilis and T. gondii and rubella infection during antenatal care among women living in Franceville, Gabon...
June 12, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28592362/toxoplasma-gondii-apoptosis-and-cellular-division-alterations-after-in-vitro-treatment-with-copper-ii-complexes
#13
J A Portes, C S Motta, N F Azeredo, C Fernandes, A Horn, W De Souza, R A DaMatta, S H Seabra
Toxoplasma gondii is the causative agent of toxoplasmosis, which is one of the most common parasitic diseases in the world. Toxoplasmosis causes severe damage to immunocompromised hosts, and the most frequently used therapy is the combination of pyrimethamine and sulfadiazine, which has side effects. Thus, there is a need for new therapies that target T. gondii. Herein, we present the anti-Toxoplasma effect of two new copper(II) complexes: [(H2L1) Cu (μ-Cl)2 Cu(H2L1)] Cl2·5H2O (1) and [(H2L2) Cu (μ-Cl)2 Cu(H2L2)] Cl2·6H2O (2)...
April 20, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28592247/immunization-with-a-dna-vaccine-encoding-toxoplasma-gondii-superoxide-dismutase-tgsod-induces-partial-immune-protection-against-acute-toxoplasmosis-in-balb-c-mice
#14
Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
BACKGROUND: Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite that infects all warm-blooded animals including humans and causes toxoplasmosis. An effective vaccine could be an ideal choice for preventing and controlling toxoplasmosis. T. gondii Superoxide dismutase (TgSOD) might participate in affecting the intracellular growth of both bradyzoite and tachyzoite forms. In the present study, the TgSOD gene was used to construct a DNA vaccine (pEGFP-SOD). METHODS: TgSOD gene was amplified and inserted into eukaryotic vector pEGFP-C1 and formed the DNA vaccine pEGFP-SOD...
June 7, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28591403/preliminary-multiplex-microarray-igg-immunoassay-for-the-diagnosis-of-toxoplasmosis-and-rubella
#15
Priscila T Baschirotto, Marco A Krieger, Leonardo Foti
BACKGROUND: During pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. Early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. OBJECTIVE: Here, we report the development of a multiplex assay for simultaneous detection of IgG antibodies produced during toxoplasmosis and rubella infection. METHODS: This assay is based on xMap technology...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28587147/toxoplasma-gondii-in-the-food-supply
#16
Malik A Hussain, Victoria Stitt, Elizabeth A Szabo, Bruce Nelan
Toxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii. Infections are usually either asymptomatic or develop mild symptoms that are self-limited, but infections in immunosuppressed persons can be severe. Infections in pregnant women can cause serious health problems in the child such as mental retardation and blindness. Infection with T. gondii in immunocompetent adults can lead to impaired eyesight. Toxoplasmosis has ranked very highly in two studies of death and disability attributable to foodborne pathogens...
May 26, 2017: Pathogens
https://www.readbyqxmd.com/read/28585421/evaluation-of-potential-anti-toxoplasmosis-efficiency-of-combined-traditional-herbs-in-a-mouse-model
#17
Xun-Hui Zhuo, Hong-Chao Sun, Bin Huang, Hai-Jie Yu, Ying Shan, Ai-Fang Du
Toxoplasma gondii is a worldwide spread protozoan and is able to infect almost all warm-blood animals. No effective drugs are available clinically on toxoplasmosis. Chinese traditional herbal medicines have provided remedies for many health problems. There exists a possibility that Chinese herbs may provide protection against T. gondii. This work aims to assess the protective efficacy of combined Chinese herbs against T. gondii. We screened five herbal medicines that have different pharmacological effects and combined them into a prescription according to the traditional Chinese medicine compatibility principle...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28578461/seroprevalence-and-risk-factors-of-toxoplasma-gondii-infection-in-invasive-raccoons-procyon-lotor-in-central-europe
#18
M Heddergott, A C Frantz, M Stubbe, A Stubbe, H Ansorge, N Osten-Sacken
Toxoplasma gondii is an obligate intracellular protozoan that causes toxoplasmosis in warm-blooded animals. Most mammals, including humans, can become intermediate host, resulting in subclinical infection or even death. Generally, there is limited information on the epidemiology of T. gondii of game species in Germany. As omnivores, raccoons, which are particularly widespread and abundant in Germany, are particularly exposed to infection the parasite. Here, we report the seroprevalence of T. gondii antibodies from 15 study sites located in Luxembourg and Germany...
June 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28573462/mast-cell-activator-compound-48-40-is-not-an-effective-adjuvant-for-uv-attenuated-toxoplasma-gondii-vaccine
#19
Xi Li, Shengjie Chen, Shiguang Huang, Fangli Lu
Toxoplasma gondii (T. gondii, Tg) is a globally distributed parasitic protozoan causing different forms of toxoplasmosis in humans. Mast cells (MCs) play a role during T. gondii infection. Several studies suggest that MC activator compound 48/80 (C48/80) may be an effective vaccine adjuvant resulting in a potent and protective antigen-specific immune response against bacteria or virus infections. The present study was performed to determine whether C48/80 had adjuvant activity for ultraviolet (UV)-attenuated T...
June 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28569215/diagnosis-of-swine-toxoplasmosis-by-pcr-and-genotyping-of-toxoplasma-gondii-from-pigs-in-henan-central-china
#20
Haiyan Wang, Longxian Zhang, Qinge Ren, Fuchang Yu, Yurong Yang
BACKGROUND: Toxoplasma gondii, a widely prevalent protozoan parasite, causes serious toxoplasmosis infections in humans and other animals. Among livestock, pigs are susceptible to T. gondii infection. Despite Henan being one of the biggest pig-raising provinces in China, little information exists on the epidemiology of toxoplasmosis in this location. Therefore, we molecularly characterized DNA samples from pigs in Henan. A total of 1647 samples, including 952 from dead piglets, 478 from seriously sick fattening pigs and 217 from abortion sows, were collected from different animal hospitals or pig farms from 10 different cities in Henan (2006-2008)...
May 31, 2017: BMC Veterinary Research
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