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https://www.readbyqxmd.com/read/28095856/baff-index-and-cxcl13-levels-in-the-cerebrospinal-fluid-associate-respectively-with-intrathecal-igg-synthesis-and-cortical-atrophy-in-multiple-sclerosis-at-clinical-onset
#1
M Puthenparampil, L Federle, S Miante, A Zito, E Toffanin, S Ruggero, M Ermani, S Pravato, D Poggiali, P Perini, F Rinaldi, P Gallo
BACKGROUND: B lymphocytes are thought to play a relevant role in multiple sclerosis (MS) pathology. The in vivo analysis of intrathecally produced B cell-related cytokines may help to clarify the mechanisms of B cell recruitment and immunoglobulin production within the central nervous system (CNS) in MS. METHODS: Paired cerebrospinal fluid (CSF) and serum specimens from 40 clinically isolated syndrome suggestive of MS or early-onset relapsing-remitting MS patients (CIS/eRRMS) and 17 healthy controls (HC) were analyzed for the intrathecal synthesis of IgG (quantitative formulae and IgG oligoclonal bands, IgGOB), CXCL13, BAFF, and IL-21...
January 17, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28075411/tamaractam-a-new-bioactive-lactam-from-tamarix-ramosissima-induces-apoptosis-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#2
Yao Yao, Cheng-Shuai Jiang, Na Sun, Wei-Qi Li, Yang Niu, Huai-Qin Han, Zhen-Hua Miao, Xun-Xia Zhao, Jing Zhao, Juan Li
Chemical investigation of Tamarix ramosissima Ledeb, a traditional herbal medicine used for rheumatoid arthritis (RA) treatment in northwest China, led to the discovery of a new phenolic aromatic rings substituted lactam, tamaractam (1), together with the previously reported compounds cis-N-feruloyl-3-O-methyldopamine (2) and trans-N-feruloyl-3-O-methyldopamine (3). The structures of the compounds were determined by high resolution electrospray ionization mass spectroscopy (HRESIMS) and 1D and 2D-NMR experiments, as well as comparison with the literature data...
January 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28053286/autophagy-defection-in-fibroblast-like-synoviocytes-lead-to-immune-enhancement-potential-in-rheumatoid-arthritis
#3
Ru Yang, Yingzi Zhang, Lin Wang, Ji Hu, Jian Wen, Leixi Xue, Mei Tang, Zhichun Liu, Jinxiang Fu
The incidence of rheumatoid arthritis (RA) has been reported to be correlated with a disorder of immunregulation. Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) play an important role in regulating the local immune microenvironment. However, the potential mechanism of RA-FLS in regulating the immnue response is not clearly understood. In this study, we demonstrated that the expression of HIF-1α was significantly up-regulated in rheumatoid arthritis tissue which indicated that the hypoxia condition in the microenvironment...
December 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/28038709/signet-ring-cell-lymphoma-clinicopathologic-immunohistochemical-and-fluorescence-in-situ-hybridization-studies-of-7-cases
#4
Shanxiang Zhang, Jihong Sun, Yanan Fang, Mehdi Nassiri, Lanting Liu, Jiehao Zhou, Ryan Stohler, Haki Choi, Gail H Vance
CONTEXT: Signet-ring cell lymphoma (SRCL) is a rare morphologic variant of non-Hodgkin lymphoma. Although it was initially reported as a rare morphologic variant of follicular lymphoma (FL), SRCL has to date been described in most types of non-Hodgkin lymphoma, mostly as single-case reports. OBJECTIVE: To study SRCL systematically by immunohistochemical stains and fluorescent in situ hybridization analyses. DESIGN: Seven SRCL cases were stained for CD3, CD5, CD20, PAX-5, CD10, CD21, CD23, cyclin D1, BCL2, BCL6, Ki-67, and MUM-1, and were analyzed by fluorescent in situ hybridization for BCL2, BCL6, MYC, and MALT1 rearrangements...
February 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/28035365/matrine-induces-the-apoptosis-of-fibroblast-like-synoviocytes-derived-from-rats-with-collagen-induced-arthritis-by-suppressing-the-activation-of-the-jak-stat-signaling-pathway
#5
Yongsheng Yang, Qiumei Dong, Rongheng Li
The induction of apoptosis-resistant rheumatoid synovial tissue cells has been related to constitutively active Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling in rheumatoid arthritis (RA). The excessive proliferation and inherent resistance to apoptosis of fibroblast-like synoviocytes (FLS) is an important mechanism by which RA originates. However, the effects of matrine on FLS in RA is unclear. The present study aimed to investigate the mechanism of action of matrine in a rat model of collagen‑induced arthritis (CIA)...
February 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28026003/quercetin-promotes-the-apoptosis-of-fibroblast-like-synoviocytes-in-rheumatoid-arthritis-by-upregulating-lncrna%C3%A2-malat1
#6
Fang Pan, Lihua Zhu, Haozhe Lv, Chunpeng Pei
Rheumatoid arthritis (RA) is a chronic autoimmune joint disease and fibroblast-like synoviocytes (FLS) are the resident mesenchymal cells of synovial joints. Quercetin is a dietary antioxidant. In this study, we aimed to explore the mechanisms responsible for the quercetin-induced apoptosis of FLS from patients with RA (termed RAFLS). RAFLS viability was determined following treatent of the cells with or without quercetin using the Cell Counting kit-8 (CCK-8) assay. The apoptosis of the RAFLS was analyzed using the Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit I...
November 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28013187/halofuginone-inhibits-tnf-%C3%AE-induced-the-migration-and-proliferation-of-fibroblast-like-synoviocytes-from-rheumatoid-arthritis-patients
#7
Shan Zeng, Kefeng Wang, Mingcheng Huang, Qian Qiu, Youjun Xiao, Maohua Shi, Yaoyao Zou, Xiuyan Yang, Hanshi Xu, Liuqin Liang
Fibroblast-like synoviocytes (FLSs) display an aggressive phenotype that is a critical factor in cartilage destruction in rheumatoid arthritis (RA). Increased FLS migration and proliferation are essential to the pathology of RA. Halofuginone has been found to inhibit cell migration and proliferation in cancer cells. However, whether halofuginone has a role in the treatment of RA FLSs is unclear. In this study, we found that halofuginone reduced migration, invasion, cell proliferation and MMPs expression in RA FLSs...
February 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28012239/secretion-of-pro-inflammatory-cytokines-and-chemokines-and-loss-of-regulatory-signals-by-fibroblast-like-synoviocytes-in-juvenile-idiopathic-arthritis
#8
AnneMarie C Brescia, Megan M Simonds, Kathleen E Sullivan, Carlos D Rose
OBJECTIVE: The goal was to investigate the specific contribution of fibroblast-like synoviocytes (FLS) to the inflammatory milieu of the synovium in juvenile idiopathic arthritis (JIA) through detection of secreted proteins. METHODS: Expression of 89 cytokines and chemokines was determined on unprocessed synovial fluid from controls and JIA patients using antibody arrays. Supernatants from pure cell cultures of FLS grown from synovial fluids or tissues from JIA and controls were also examined for protein expression...
December 23, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28011260/cost-comparison-of-fundamentals-of-laparoscopic-surgery-training-completed-with-standard-fundamentals-of-laparoscopic-surgery-equipment-versus-low-cost-equipment
#9
Brenton R Franklin, Sarah B Placek, Mercy D Wagner, Sarah M Haviland, Mary T O'Donnell, E Matthew Ritter
OBJECTIVE: Training for the Fundamentals of Laparoscopic Surgery (FLS) skills test can be expensive. Previous work demonstrated that training on an ergonomically different, low-cost platform does not affect FLS skills test outcomes. This study compares the average training cost with standard FLS equipment and medical-grade consumables versus training on a lower cost platform with non-medical-grade consumables. DESIGN: Subjects were prospectively randomized to either the standard FLS training platform (n = 19) with medical-grade consumables (S-FLS), or the low-cost platform (n = 20) with training-grade products (LC-FLS)...
December 20, 2016: Journal of Surgical Education
https://www.readbyqxmd.com/read/28004297/evaluation-of-the-first-fracture-liaison-service-in-the-greek-healthcare-setting
#10
Polyzois Makras, Maria Panagoulia, Andriana Mari, Stavroula Rizou, George P Lyritis
: We evaluated the first implementation of FLS in the Greek healthcare setting, at the 251 Hellenic Air Force and VA General Hospital of Athens. Participation rate was moderate (54.5%) and needs improvement; osteoporosis medication was either suggested or reviewed in 74 out of the 116 patients recruited. PURPOSE: The purpose of this study was to evaluate the first implementation of a fracture liaison service (FLS) in Greece, at the 251 Hellenic Air Force and VA General Hospital, Athens...
December 2017: Archives of Osteoporosis
https://www.readbyqxmd.com/read/28002318/brahma-related-gene-1-induces-apoptosis-in-a-p53-dependent-manner-in-human-rheumatoid-fibroblast-like-synoviocyte-mh7a
#11
Hongli Hou, Weipeng Xing, Wuyin Li
Blocked apoptosis and aggressive inflammatory responses occur in fibroblast-like synoviocyte (FLS) of rheumatoid arthritis (RA) patients. Although Brahma-related gene 1 (BRG1) is considered as a tumor suppressor, few research covers its role in RA. This study aims to reveal effects and potential mechanisms of BRG1 in human FLS cell line MH7A.BRG1 expression in MH7A cells was altered by transfection of overexpression vectors or short hairpin RNAs (shRNAs). Cell viability and apoptosis were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry after transfection...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27995997/tiarp-attenuates-autoantibody-mediated-arthritis-via-the-suppression-of-neutrophil-migration-by-reducing-cxcl2-cxcr2-and-il-6-expression
#12
Asuka Inoue, Isao Matsumoto, Yuki Tanaka, Naoto Umeda, Chinatsu Takai, Hoshimi Kawaguchi, Hiroshi Ebe, Hiroto Yoshida, Yoshihiro Matsumoto, Seiji Segawa, Satoru Takahashi, Takayuki Sumida
TNFα-induced adipose-related protein (TIARP) is a six-transmembrane protein expressed on macrophages, neutrophils and synoviocytes. We reported recently that mice deficient in TIARP (TIARP(-/-)) spontaneously develop arthritis and are highly susceptible to collagen-induced arthritis (CIA) with enhanced interleukin (IL)-6 production. However, the effects of TIARP on neutrophils and fibroblast-like synoviocytes (FLS) have not been elucidated. We analyzed the roles of TIARP in K/BxN serum transfer model using TIARP(-/-) mice...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27992314/cytotoxic-and-genotoxic-effects-of-electronic-cigarette-liquids-on-human-mucosal-tissue-cultures-of-the-oropharynx
#13
Christian Welz, Martin Canis, Sabina Schwenk-Zieger, Sven Becker, Vincent Stucke, Friedrich Ihler, Philipp Baumeister
The popularity of electronic cigarettes (ECs) is rapidly growing and ECs are claimed to be an uncritically regarded alternative to conventional cigarettes. The mucosal tissue of the upper aerodigestive tract (UADT) is the first contact organ for xenobiotics such as liquids of ECs. The aim of this study is to investigate the bimolecular effects of e-liquids on human pharyngeal tissue cultures to evaluate whether e-liquids and their components present a risk factor for head and neck squamous cell carcinoma. Fresh tissue samples of healthy oropharyngeal mucosa were assembled into mucosal tissue cultures...
2016: Journal of Environmental Pathology, Toxicology and Oncology
https://www.readbyqxmd.com/read/27956357/a-survey-of-herbal-weeds-that-are-used-to-treat-gastrointestinal-disorders-from-southern-thailand-krabi-and-songkhla-provinces
#14
Oratai Neamsuvan, Thamakorn Ruangrit
ETHNOPHARMACOLOGICAL RELEVANCE: Weeds are plants grow naturally and are commonly seen. They are mostly used for feedstuff. However, their use as herbs for treating diseases, including gastrointestinal disorders, is rare. Therefore, the present study aimed to: (1) quantify the number of herbal weeds used for treating gastrointestinal disorders; (2) study local knowledge of weed utilization for treating gastrointestinal disorders in Songkhla and Krabi provinces; and (3) analyse quantitative data with the Informant Consensus Factor (ICF), Use Value (UV) and Fidelity Level (FL)...
January 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27936399/gastrodia-elata-attenuates-inflammatory-response-by-inhibiting-the-nf-%C3%AE%C2%BAb-pathway-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#15
Yu Li, Li-Min Wang, Jian-Zhong Xu, Ke Tian, Chen-Xi Gu, Zhi-Fu Li
Gastrodia elata (GE), which belongs to the Orchidaceae family, was found to possess anti-inflammatory activity. However, the effect of GE on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) remains largely unknown. Thus, the aim of this study was to investigate the effects of GE on tumor necrosis factor-α (TNF-α)-induced inflammatory response in RA-FLS and the underlying molecular mechanism was also explored. Our results demonstrated that GE significantly attenuated TNF-α-induced IL-6 and IL-8 production in RA-FLS...
January 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27928664/laparoscopic-skill-assessment-of-practicing-surgeons-prior-to-enrollment-in-a-surgical-trial-of-a-new-laparoscopic-procedure
#16
Benjamin Zendejas, James W Jakub, Alicia M Terando, Amod Sarnaik, Charlotte E Ariyan, Mark B Faries, Sabino Zani, Heather B Neuman, Nabil Wasif, Jeffrey M Farma, Bruce J Averbook, Karl Y Bilimoria, Douglas Tyler, Mary Sue Brady, David R Farley
BACKGROUND: Outcomes of surgical trials hinge on surgeon selection and their underlying expertise. Assessment of expertise is paramount. We investigated whether surgeons' performance measured by the fundamentals of laparoscopic surgery (FLS) assessment program could predict their performance in a surgical trial. METHODS: As part of a prospective multi-institutional study of minimally invasive inguinal lymphadenectomy (MILND) for melanoma, surgical oncologists with no prior MILND experience underwent pre-trial FLS assessment...
December 7, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27908500/effectiveness-of-a-comprehensive-mental-skills-curriculum-in-enhancing-surgical-performance-results-of-a-randomized-controlled-trial
#17
Dimitrios Stefanidis, Nicholas E Anton, Lisa D Howley, Eric Bean, Ashley Yurco, Manuel E Pimentel, Cameron K Davis
INTRODUCTION: We hypothesized that the implementation of a novel mental skills curriculum (MSC) during laparoscopic simulator training would improve mental skills and performance, and decrease stress. METHODS: Sixty volunteer novices were randomized into intervention and control groups. All participants received FLS training while the intervention group also participated in the MSC. Skill transfer and retention were assessed on a live porcine model after training and 2 months later, respectively...
November 22, 2016: American Journal of Surgery
https://www.readbyqxmd.com/read/27908299/the-infrapatellar-fat-pad-induces-inflammatory-and-degradative-effects-in-articular-cells-but-not-through-leptin-or-adiponectin
#18
Jean-Baptiste Gross, Cecile Guillaume, Pascale Gegout-Pottie, Pascal Reboul, Jean-Yves Jouzeau, Didier Mainard, Nathalie Presle
OBJECTIVES: Based on a novel approach suggesting a role of adipose tissue in osteoarthritis (OA), we aimed to determine whether the infrapatellar fat pad (IFP) may affect joint cell functions through adipokines. METHODS: The conditioned media of IFP and subcutaneous adipose tissue from OA patients were used to determine the production of leptin and adiponectin, and to stimulate chondrocytes and fibroblast-like synoviocytes (FLS). Blocking experiments were carried out to evaluate the contribution of adipokines to IFP effects...
November 13, 2016: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/27903245/the-predictive-value-of-sonographic-images-of-follicular-lesions-a-comparison-with-nodules-unequivocal-in-fna-single-centre-prospective-study
#19
Dorota Słowińska-Klencka, Martyna Wojtaszek-Nowicka, Stanisław Sporny, Ewa Woźniak-Oseła, Bożena Popowicz, Mariusz Klencki
BACKGROUND: To determine the diagnostic efficacy of ultrasonographic malignancy risk features (UMRFs) in follicular lesions (FL) in a population with low risk of malignancy in FL and to compare it with a similar analysis in a group of patients with unequivocal cytology (UC): benign lesion (BL) or malignant neoplasm (MN). METHODS: Presence of UMRFs (hypoechogenicity, solid echostructure, taller-than-wide shape, pathological vascularization, irregular margins, microcalcifications and macrocalcifications) and their sets were assessed in 322 FL: 202 follicular lesions of undetermined significance (FLUS) and 120 suspicious for follicular neoplasm (SFN) and 300 nodules with UC: 200 BL and 100 MN, subsequently evaluated histopathologically...
December 1, 2016: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/27894399/effect-of-iguratimod-and-methotrexate-on-rankl-and-opg-expression-in-serum-and-il-1%C3%AE-induced-fibroblast-like-synoviocytes-from-patients-with-rheumatoid-arthritis
#20
X T Wang, P Li, T S Xu, R Ding, X Zhang, L Q Bi
The receptor activator of nuclear factor κB ligand (RANKL)/receptor activator of nuclear factor κB (RANK)/osteoprotegerin (OPG) system plays a key role in rheumatoid arthritis (RA)-associated bone erosion. The upregulation of the RANKL/OPG ratio promotes bone erosion. The objective of this study is to explore the effects of iguratimod, a small-molecule disease-modifying antirheumatic drug (DMARD), alone or in combination with methotrexate (MTX), on RANKL and OPG expression in RA. We performed an enzyme-linked immunosorbent assay (ELISA) to investigate the modulatory effects of iguratimod, MTX, or their combination on serum RANKL and OPG levels of patients with RA before and after treatment for 12 and 24 weeks...
October 31, 2016: Cellular and Molecular Biology
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