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https://www.readbyqxmd.com/read/28649674/synovial-fibroblast-neutrophil-interactions-promote-pathogenic-adaptive-immunity-in-rheumatoid-arthritis
#1
Carmelo Carmona-Rivera, Philip M Carlucci, Erica Moore, Nithya Lingampalli, Hannes Uchtenhagen, Eddie James, Yudong Liu, Kevin L Bicker, Heidi Wahamaa, Victoria Hoffmann, Anca Irinel Catrina, PaulR Thompson, Jane H Buckner, William H Robinson, David A Fox, Mariana J Kaplan
Rheumatoid arthritis (RA) is characterized by synovial joint inflammation and by development of pathogenic humoral and cellular autoimmunity to citrullinated proteins. Neutrophil extracellular traps (NETs) are a source of citrullinated autoantigens and activate RA synovial fibroblasts (FLS), cells crucial in joint damage. We investigated the molecular mechanisms by which NETs promote proinflammatory phenotypes in FLS, and whether these interactions generate pathogenic anti-citrulline adaptive immune responses...
April 2017: Science Immunology
https://www.readbyqxmd.com/read/28643066/solving-the-surgeon-ergonomic-crisis-with-surgical-exosuit
#2
Shanglei Liu, Daniel Hemming, Ran B Luo, Jessica Reynolds, Jonathan C Delong, Bryan J Sandler, Garth R Jacobsen, Santiago Horgan
INTRODUCTION: The widespread adoption of laparoscopic surgery has put new physical demands on the surgeon leading to increased musculoskeletal disorders and injuries. Shoulder, back, and neck pains are among the most common complaints experienced by laparoscopic surgeons. Here, we evaluate the feasibility and efficacy of a non-intrusive progressive arm support exosuit worn by surgeons under the sterile gown to reduce pain and fatigue during surgery. METHODS AND PROCEDURES: This is a prospective randomized crossover study approved by the Internal Review Board (IRB)...
June 22, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28631608/one-year-in-review-2017-pathogenesis-of-rheumatoid-arthritis
#3
REVIEW
Francesca Angelotti, Alice Parma, Giacomo Cafaro, Riccardo Capecchi, Alessia Alunno, Ilaria Puxeddu
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease influenced by both genetic and environmental factors. It has been postulated that a high-risk genetic background, in combination with epigenetic marks and environmental exposures, leads to a cascade of events inducing synovitis and consequent destructive arthritis. The clinical picture of joint involvement in RA is the result of chronic inflammation of the synovium, characterised by interactions of resident cells such as fibroblast-like synoviocytes (FLS) with cells of the innate (e...
May 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28624174/the-performance-gap-for-residents-in-transfer-of-intracorporeal-suturing-skills-from-box-trainer-to-operating-room
#4
Patrice Crochet, Aubert Agostini, Sophie Knight, Noémie Resseguier, Stéphane Berdah, Rajesh Aggarwal
OBJECTIVE: Training on laparoscopic box trainer (BT) improves suturing skills in the operating room (OR). Optimal laboratory training should provide trainees with an adequate level of skills that can be transferred to the OR with no decline in performances. This study aimed to compare OR intracorporeal suturing performances of residents who trained with a fundamentals of laparoscopic surgery (FLS) BT vs. those of experienced laparoscopists. DESIGN: This was a prospective observational study...
June 14, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28618161/prevention-of-osteoporotic-refractures-in-regional-australia
#5
Emily Davidson, Alexa Seal, Zelda Doyle, Kerin Fielding, Joe McGirr
OBJECTIVE: Clinical guidelines recommend that patients who sustain a minimal trauma fracture (MTF) should receive a bone mineral density (BMD) scan and bisphosphonate (or equivalent) therapy if diagnosed with osteoporosis. A pilot fracture liaison service (FLS) was implemented in regional NSW to improve adherence to the guidelines. DESIGN: Prospective cohort study with an historical control. SETTING: Primary care. PARTICIPANTS: Control (n = 47) and cohort (n = 93) groups comprised patients consenting to interview who presented with a MTF to the major referral hospital 4 months before and 12 months after FLS implementation respectively...
June 15, 2017: Australian Journal of Rural Health
https://www.readbyqxmd.com/read/28617559/blockade-of-il-17-alleviated-inflammation-in-rat-arthritis-and-mmp-13-expression
#6
X-L Shui, W Lin, C-W Mao, Y-Z Feng, J-Z Kong, S-M Chen
OBJECTIVE: Rheumatoid arthritis (RA) is one systemic auto-immune disorder featured as chronic synovitis and can destruct joint cartilage. Fibroblast-like synoviocyte (FLS) secretes various factors affecting chondrocyte matrix and degradation. This study thus investigated the effect of interleukin-17A (IL-17A) on FLS and osteoclast. MATERIALS AND METHODS: Type II collagen-induced arthritis (CIA) rats were assigned to CIA model, CIA + IgG1 isotype, and CIA + Anti-Rat IL-17A groups...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28603383/foreign-language-reading-and-spelling-in-gifted-students-with-dyslexia-in-secondary-education
#7
Sietske van Viersen, Elise H de Bree, Lilian Kalee, Evelyn H Kroesbergen, Peter F de Jong
A few studies suggest that gifted children with dyslexia have better literacy skills than averagely intelligent children with dyslexia. This finding aligns with the hypothesis that giftedness-related factors provide compensation for poor reading. The present study investigated whether, as in the native language (NL), the level of foreign language (FL) literacy of gifted students with dyslexia is higher than the literacy level of averagely intelligent students with dyslexia and whether this difference can be accounted for by the difference in their NL literacy level...
2017: Reading and Writing
https://www.readbyqxmd.com/read/28587985/naturally-occurring-xanthone-and-benzophenone-derivatives-exert-significant-anti-proliferative-and-proapoptotic-effects-in-vitro-on-synovial-fibroblasts-and-macrophages-from-rheumatoid-arthritis-patients
#8
Izabella Henc, Adam Kokotkiewicz, Piotr Łuczkiewicz, Ewa Bryl, Maria Łuczkiewicz, Jacek M Witkowski
There is a need for novel, safer and cheaper drugs for the therapy of rheumatoid arthritis (RA), better targeted against the cellular processes involved in the disease pathogenesis. Using advanced analysis of microscopic images and flow cytometry, we demonstrate that naturally occurring xanthone and benzophenone derivatives exert strong, dose- and O2 concentration-dependent anti-proliferative and pro-apoptotic effects on RA patients' fibroblast-like synoviocytes (FLS) and macrophages. Suspensions containing fibroblasts, macrophages and other infiltrating cells were obtained from inflamed synovial tissue collected from female RA patients...
June 3, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28573530/mecp2-regulates-ptch1-expression-through-dna-methylation-in-rheumatoid-arthritis
#9
Zheng-Hao Sun, Yan-Hui Liu, Jun-da Liu, Dan-Dan Xu, Xiao-Feng Li, Xiao-Ming Meng, Tao-Tao Ma, Cheng Huang, Jun Li
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease, in which pathogenesis is not clear. Many research demonstrated that fibroblast-like synoviocytes (FLSs) play a key role in RA pathogenesis, join in the cartilage injury and hyperplasia of the synovium, and contribute to the release of inflammatory cytokines. We used adjuvant arthritis (AA) rats as RA animal models. The methyl-CpG-binding protein 2 (MeCP2) enables the suppressed chromatin structure to be selectively detected in AA FLSs. Overexpression of this protein leads to an increase of integral methylation levels...
June 1, 2017: Inflammation
https://www.readbyqxmd.com/read/28569176/fibroblast-like-synoviocyte-metabolism-in-the-pathogenesis-of-rheumatoid-arthritis
#10
REVIEW
Marta F Bustamante, Ricard Garcia-Carbonell, Katrijn D Whisenant, Monica Guma
An increasing number of studies show how changes in intracellular metabolic pathways alter tumor and immune cell function. However, little information about metabolic changes in other cell types, including synovial fibroblasts, is available. In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLS) are the most common cell type at the pannus-cartilage junction and contribute to joint destruction through their production of cytokines, chemokines, and matrix-degrading molecules and by migrating and invading joint cartilage...
May 31, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28559961/autophagy-inhibitor-regulates-apoptosis-and-proliferation-of-synovial-fibroblasts-through-the-inhibition-of-pi3k-akt-pathway-in-collagen-induced-arthritis-rat-model
#11
Shu Li, Jin-Wei Chen, Xi Xie, Jing Tian, Cong Deng, Jia Wang, Hai-Na Gan, Fen Li
BACKGROUND AND OBJECTIVE: Mounting studies have illustrated an important role of autophagy in various diseases, but few studies have reported its contribution to rheumatoid arthritis (RA) and the underlying mechanism was largely unknown. This study aimed to investigate whether autophagy inhibitors could regulate apoptosis and proliferation through PI3K/AKT pathway in RA. METHODS: RA animal model was established by collagen induction. General observations and degree of joint swelling were observed...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28550307/therapeutic-effects-of-matrine-derivate-masm-in-mice-with-collagen-induced-arthritis-and-on-fibroblast-like-synoviocytes
#12
Yuming Zou, Quan Li, Denghui Liu, Jia Li, Qing Cai, Chao Li, Qingjie Zhao, Weidong Xu
MASM is a matrine derivate that exhibits a number of pharmacological effects, including immunosuppressive activity and anti-inflammatory properties. In this study, the mechanisms underlying the therapeutic efficacy of MASM in the treatment of rheumatoid arthritis were investigated using DBA/1 mice with collagen-induced arthritis (CIA) and fibroblast-like synoviocytes derived from rheumatoid arthritis patients (RA-FLS). We demonstrated that MASM markedly attenuated the severity of arthritis in CIA mice. The therapeutic effects were associated with ameliorated joint swelling and reduced bone erosion and destruction...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28537403/comparative-analysis-of-two-flavonol-synthases-from-different-colored-onions-provides-insight-into-flavonoid-biosynthesis
#13
Sangkyu Park, Da-Hye Kim, Jong-Yeol Lee, Sun-Hwa Ha, Sun-Hyung Lim
We isolated cDNAs encoding flavonol synthase (FLS) from the red onion 'H6' (AcFLS-H6) and the yellow onion 'Hwangryongball' (AcFLS-HRB). We found three amino acid variations between the two sequences. Kinetic analysis with recombinant proteins revealed that AcFLS-HRB exhibited approximately 2-fold higher catalytic efficiencies than AcFLS-H6 for dihydroflavonol substrates, and both proteins preferred dihydroquercetin to dihydrokaempferol. The expression patterns of flavonoid biosynthesis genes corresponded to the accumulation patterns of flavonoid aglycones in both onions...
May 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28536889/the-phenotype-of-patients-with-a-recent-fracture-a-literature-survey-of-the-fracture-liaison-service
#14
REVIEW
Lisanne Vranken, Caroline E Wyers, Joop P W van den Bergh, Piet P M M Geusens
The aetiology of fractures in patients aged 50 years and older is multifactorial, and includes bone- and fall-related risks. The Fracture Liaison Service (FLS) is recommended to identify patients with a recent fracture and to evaluate their subsequent fracture risk, in order to take measures to decrease the risk of subsequent fractures in patients with a high risk phenotype. A literature survey was conducted to describe components of the bone- and fall-related phenotype of patients attending the FLS. Components of the patient phenotype at the FLS have been reported in 33 studies...
May 24, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28533268/the-transcription-factor-gli3-promotes-b-cell-development-in-fetal-liver-through-repression-of-shh
#15
Anisha Solanki, Ching-In Lau, José Ignacio Saldaña, Susan Ross, Tessa Crompton
Before birth, B cells develop in the fetal liver (FL). In this study, we show that Gli3 activity in the FL stroma is required for B cell development. In the Gli3-deficient FL, B cell development was reduced at multiple stages, whereas the Sonic hedgehog (Hh [Shh])-deficient FL showed increased B cell development, and Gli3 functioned to repress Shh transcription. Use of a transgenic Hh-reporter mouse showed that Shh signals directly to developing B cells and that Hh pathway activation was increased in developing B cells from Gli3-deficient FLs...
May 22, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28528333/effect-of-1-25-oh-2d3-on-proliferation-of-fibroblast-like-synoviocytes-and-expressions-of-pro-inflammatory-cytokines-through-regulating-microrna-22-in-a-rat-model-of-rheumatoid-arthritis
#16
Ping Fan, Lan He, Nan Hu, Jing Luo, Jing Zhang, Ling-Fei Mo, Yan-Hua Wang, Dan Pu, Xiao-Hong Lv, Zhi-Ming Hao, Chang-Hai Ding, Wu-Jun Xue, Yang Li
OBJECTIVE: This study aims to investigate the regulatory mechanism of 1,25-(OH)2D3 on the proliferation of fibroblast-like synoviocytes (FLS) and expressions of pro-inflammatory cytokines in rheumatoid arthritis (RA) rats via microRNA-22 (miR-22). METHODS: A rat model of RA was established with a subcutaneous injection of type II collagen. After treated with different concentrations of 1,25-(OH)2D3 the proliferation of FLS was estimated by the MTT method, and the optimal concentration of 1,25-(OH)2D3 was selected for further experiments...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28520776/proteomic-analysis-of-tea-plants-camellia-sinensis-with-purple-young-shoots-during-leaf-development
#17
Qiongqiong Zhou, Zhidan Chen, Jinwook Lee, Xinghui Li, Weijiang Sun
Tea products made from purple leaves are highly preferred by consumers due to the health benefits. This study developed a proteome reference map related to color changes during leaf growth in tea (Camellia sinensis) plant with purple young shoots using two-dimensional electrophoresis (2-DE). Forty-six differentially expressed proteins were detected in the gel and successfully identified by using MALDI-TOF/TOF-MS. The pronounced changes in the proteomic profile between tender purple leaves (TPL) and mature green leaves (MGL) included: 1) the lower activity of proteins associated with CO2 assimilation, energy metabolism and photo flux efficiency and higher content of anthocyanins in TPL than those in MGL may protect tender leaves against photo-damage; 2) the higher abundance of chalcone synthase (CHS), chalcone isomerase (CHI) and flavonol synthase (FLS) likely contributes to the synthesis of anthocyanins, catechins and flavonols in TPL tissues; 3) higher abundance of stress response proteins, such as glutathione S-transferases (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx), could enhance the tolerance of TPL tissues to adverse condition in; and 4) the increased abundance of proteins related to protein synthesis, nucleic acids and cell wall proteins should be beneficial for the proliferation and expansion of leaf cell in TPL tissues...
2017: PloS One
https://www.readbyqxmd.com/read/28516302/the-minimally-acceptable-classification-criterion-for-surgical-skill-intent-vectors-and-separability-of-raw-motion-data
#18
Rodney L Dockter, Thomas S Lendvay, Robert M Sweet, Timothy M Kowalewski
PURPOSE: Minimally invasive surgery requires objective methods for skill evaluation and training. This work presents the minimally acceptable classification (MAC) criterion for computational surgery: Given an obvious novice and an obvious expert, a surgical skill evaluation classifier must yield 100% accuracy. We propose that a rigorous motion analysis algorithm must meet this minimal benchmark in order to justify its cost and use. METHODS: We use this benchmark to investigate two concepts: First, how separable is raw, multidimensional dry laboratory laparoscopic motion data between obvious novices and obvious experts? We utilized information theoretic techniques to analytically address this...
May 18, 2017: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/28509639/factors-associated-with-simulator-assessed-laparoscopic-surgical-skills-of-veterinary-students
#19
Jessica J Kilkenny, Ameet Singh, Carolyn L Kerr, Deep K Khosa, Boel A Fransson
OBJECTIVE To determine whether simulator-assessed laparoscopic skills of veterinary students were associated with training level and prior experience performing nonlaparoscopic veterinary surgery and other activities requiring hand-eye coordination and manual dexterity. DESIGN Experiment. SAMPLE 145 students without any prior laparoscopic surgical or fundamentals of laparoscopic surgery (FLS) simulator experience in years 1 (n = 39), 2 (34), 3 (39), and 4 (33) at a veterinary college. PROCEDURES A questionnaire was used to collect data from participants regarding experience performing veterinary surgery, playing video games, and participating in other activities...
June 1, 2017: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/28501116/usefulness-of-virtual-reality-in-assessment-of-medical-student-laparoscopic-skill
#20
Josh Matzke, Craig Ziegler, Kevin Martin, Stuart Crawford, Erica Sutton
BACKGROUND: This study evaluates if undergraduate medical trainees' laparoscopic skills acquisition could be assessed using a virtual reality (VR) simulator and how the resultant metrics correlate with performance of Fundamentals of Laparoscopic Surgery (FLS) tasks. Our hypothesis is that the VR simulator metrics will correlate with passing results in a competency-based curriculum (FLS). MATERIALS AND METHODS: Twenty-eight fourth-year medical students applying for surgical residency were recruited to participate in a VR training curriculum comprised of camera navigation, hand eye coordination, and FLS tasks: circle cutting (CC), ligating loop (LL), peg transfer (PT), and intracorporeal knot tying (IKT)...
May 1, 2017: Journal of Surgical Research
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