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https://www.readbyqxmd.com/read/29137228/a-native-like-bispecific-antibody-suppresses-the-inflammatory-cytokine-response-by-simultaneously-neutralizing-tumor-necrosis-factor-alpha-and-interleukin-17a
#1
Tianshu Xu, Tianlei Ying, Lili Wang, Xiaohua Douglas Zhang, Ying Wang, Lishan Kang, Tao Huang, Liang Cheng, Liping Wang, Qi Zhao
Anti-tumor necrosis factor (TNF) therapies are successful in the treatment of inflammatory disorders. However, some patients with rheumatoid arthritis (RA) fail to response anti-TNF drugs due to the compensation of other inflammatory signals. In this study, to reduce compensatory responses of interleukin-17A (IL-17A) during TNF-α inhibition, we generated an IgG-like bispecific antibodiy (bsAb) against TNF-α and IL-17A through a combination method of electrostatic Fc pairing and light chain crossover. This bsAb exhibited relatively high stability comparable to natural IgG antibodies, and retained the unaltered affinities to both of two targets...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29119225/piperlongumine-inhibits-the-proliferation-migration-and-invasion-of-fibroblast-like-synoviocytes-from-patients-with-rheumatoid-arthritis
#2
Siqi Xu, Youjun Xiao, Shan Zeng, Yaoyao Zou, Qian Qiu, Mingcheng Huang, Zhongping Zhan, Liuqin Liang, Xiuyan Yang, Hanshi Xu
OBJECTIVES: Recent studies have indicated that piperlongumine (PLM) may exert anti-inflammatory effects. In the present study, we determined the effect of PLM on the proliferation, apoptosis, migration and invasion of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) (referred to herein as RA FLS). We further explored the mechanisms by which the studied compound inhibits the functions of RA FLS. METHODS: RA FLS viability and apoptosis were tested using MTT and Annexin V/PI assays, respectively...
November 8, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/29116863/hypothermia-promotes-cell-protective-and-chondroprotective-effects-after-blunt-cartilage-trauma
#3
Jana Riegger, Madeleine Zimmermann, Helga Joos, Thomas Kappe, Rolf E Brenner
BACKGROUND: Cryotherapy is routinely administered after sports injuries of synovial joints. Although positive clinical effects on periarticular swelling and pain have been described, the effects on the cell biological activities of cartilage and synovial cells remain largely unknown so far. HYPOTHESIS: Local hypothermia alleviates synovial reactions and prevents chondrocyte death as well as cartilage destructive processes after blunt cartilage trauma. STUDY DESIGN: Controlled laboratory study...
November 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29115552/effects-of-daphnetin-combined-with-bcl2%C3%A2-sirna-on-antiapoptotic-genes-in-synovial-fibroblasts-of-rats-with-collagen%C3%A2-induced-arthritis
#4
Xiaoying Chen, Nanzhen Kuang, Xiaoping Zeng, Zhiqin Zhang, Yanyan Li, Wei Liu, Yingyuan Fu
The aim of the present study was to investigate the effects of daphnetin combined with B cell lymphoma 2 (Bcl2)‑targeted small interfering (si)RNA (si‑Bcl2) on antiapoptotic genes in fibroblast‑like synoviocytes (FLS) in rats with collagen II‑induced arthritis (CIA). The roles of si‑Bcl2 and daphnetin were determined by measuring the expression levels of Bcl2. Protein and mRNA expression levels of Bcl2 in FLS were determined by flow cytometry and reverse transcription‑quantitative polymerase chain reaction...
November 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29105345/estradiol-inhibits-nlrp3-inflammasome-in-fibroblast-like-synoviocytes-activated-by-lipopolysaccharide-and-adenosine-triphosphate
#5
Jianfeng Shi, Weibo Zhao, Haijian Ying, Ying Zhang, Juping Du, Shuaishuai Chen, Jun Li, Bo Shen
OBJECTIVE: Nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3) inflammasome is known for activating pro-inflammatory cytokines in knee osteoarthritis (OA). This study was performed to identify whether NLRP3 inflammasome can be triggered by lipopolysaccharides (LPS) and adenosine triphos adenine (ATP), which are positively related with knee OA severity in joint-spaces, in human fibroblast-like synoviocytes (FLS), and to identify whether estrogen would inhibit the activation of NLRP3 inflammasome...
November 3, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/29097930/a-first-report-on-18-f-fprgd2-pet-ct-imaging-in-multiple-myeloma
#6
Nadia Withofs, François Cousin, Bernard De Prijck, Christophe Bonnet, Roland Hustinx, Sanjiv S Gambhir, Yves Beguin, Jo Caers
An observational study was set up to assess the feasibility of [(18)F]FPRGD2 PET/CT for imaging patients with multiple myeloma (MM) and to compare its detection rate with low dose CT alone and combined [(18)F]NaF/[(18)F]FDG PET/CT images. Four patients (2 newly diagnosed patients and 2 with relapsed MM) were included and underwent whole-body PET/CT after injection of [(18)F]FPRGD2. The obtained images were compared with results of low dose CT and already available results of a combined [(18)F]NaF/[(18)F]FDG PET/CT...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29082051/primary-cutaneous-follicle-centre-lymphoma-with-hodgkin-and-reed-sternberg-like-cells-a-case-report-and-review-of-the-literature
#7
Fatima A Aldarweesh, Diana O Treaba
An elderly woman with a complex medical history presented with a left forearm mass that slowly developed for several months. The excisional biopsy of this skin mass was remarkable for involvement by a follicle centre cell derived lymphoma with a nodular and diffuse pattern associated with a subset of scattered Hodgkin and Reed-Sternberg like cells. Fluorescence in situ hybridization studies did not detect the presence of IgH-bcl2 fusion transcript, and molecular studies were negative for immunoglobulin heavy chain gene rearrangements and EBV DNA sequences...
2017: Case Reports in Hematology
https://www.readbyqxmd.com/read/29070849/high-risk-follicular-lymphomas-harbour-more-somatic-mutations-including-those-in-the-aid-motif
#8
Taku Tsukamoto, Masakazu Nakano, Ryuichi Sato, Hiroko Adachi, Miki Kiyota, Eri Kawata, Nobuhiko Uoshima, Satoru Yasukawa, Yoshiaki Chinen, Shinsuke Mizutani, Yuji Shimura, Tsutomu Kobayashi, Shigeo Horiike, Akio Yanagisawa, Masafumi Taniwaki, Kei Tashiro, Junya Kuroda
We investigated clinical and genetic characteristics of high-risk follicular lymphoma (FL), that lacked evidence of large cell transformation at diagnosis, in the rituximab era. First, we retrospectively analysed the clinical features of 100 patients with non-transformed FL that were consecutively treated with rituximab-containing therapies in a discovery cohort. The presence of either peripheral blood and/or bone involvement was associated with short progression-free survival. This was confirmed in a validation cohort of 66 FL patients...
October 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29067579/trends-in-the-fundamentals-of-laparoscopic-surgery%C3%A2-fls-certification-exam-over-the-past-9%C3%A2-years
#9
Elif Bilgic, Pepa Kaneva, Allan Okrainec, E Matthew Ritter, Steven D Schwaitzberg, Melina C Vassiliou
BACKGROUND: The Fundamentals of Laparoscopic Surgery® (FLS) certification exam assesses both cognitive and manual skills, and has been administered for over a decade. The purpose of this study is to report results over the past 9 years of testing in order to identify trends over time and evaluate the need to update scoring practices. This is a quality initiative of the SAGES FLS committee. METHODS: A representative sample of FLS exam data from 2008 to 2016 was analyzed...
October 24, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/29063214/geisinger-high-risk-osteoporosis-clinic-hiroc-2013-2015-fls-performance-analysis
#10
P Dunn, D Webb, T P Olenginski
Geisinger Health System (GHS) high-risk osteoporosis clinic (HiROC), which treats patients with low-trauma, fragility fractures, reports their 2013-2015 performance measures in secondary fracture prevention. This fracture liaison service (FLS) pathway treats 75% of high-risk, drug eligible patients, compared to 13.8% in GHS primary care. This performance points to the need for more FLS programs throughout the world. INTRODUCTION: The purpose of this study is to analyze and report ongoing performance measures in outpatient and inpatient high-risk osteoporosis clinic (HiROC) program designed for patients with low-trauma, fragility fractures...
October 23, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/29052071/validation-of-the-vblast-pattern-cutting-task-a-learning-curve-study
#11
Ali M Linsk, Kimberley R Monden, Ganesh Sankaranarayanan, Woojin Ahn, Daniel B Jones, Suvranu De, Steven D Schwaitzberg, Caroline G L Cao
BACKGROUND: Mastery of laparoscopic skills is essential in surgical practice and requires considerable time and effort to achieve. The Virtual Basic Laparoscopic Skill Trainer (VBLaST-PC(©)) is a virtual simulator that was developed as a computerized version of the pattern cutting (PC) task in the Fundamentals of Laparoscopic Surgery (FLS) system. To establish convergent validity for the VBLaST-PC(©), we assessed trainees' learning curves using the cumulative summation (CUSUM) method and compared them with those on the FLS...
October 19, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/29046441/structure-function-dissection-of-the-pseudorabies-virus-glycoprotein-b-fusion-loops
#12
Melina Vallbracht, Delphine Brun, Matteo Tassinari, Marie-Christine Vaney, Gérard Pehau-Arnaudet, Pablo Guardado-Calvo, Ahmed Haouz, Barbara G Klupp, Thomas C Mettenleiter, Felix A Rey, Marija Backovic
Conserved across the Herpesviridae family, glycoprotein B (gB) is responsible for driving fusion of the viral envelope with the host cell membrane for entry upon receptor binding and activation by the viral gH/gL complex. Although crystal structures of the gB ectodomain of several herpesviruses have been reported, the membrane fusion mechanism has remained elusive. Here, we report the X-ray structure of the Pseudorabies virus (PrV) gB ectodomain, revealing a typical class III post-fusion trimer that binds membranes via its fusion loops (FLs) in a cholesterol-dependent manner...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29044208/growth-factors-regulate-phospholipid-biosynthesis-in-human-fibroblast-like-synoviocytes-obtained-from-osteoarthritic-knees
#13
Katarzyna D Sluzalska, Gerhard Liebisch, Jochen Wilhelm, Bernd Ishaque, Holger Hackstein, Gerd Schmitz, Markus Rickert, Juergen Steinmeyer
Elevated levels of growth factors and phospholipids (PLs) have been found in osteoarthritic synovial fluid (SF), although the metabolic regulation of PLs is currently unknown. This study aimed to determine the effects of growth factors on the biosynthesis of PLs by fibroblast-like synoviocytes (FLS) obtained from human osteoarthritic knee joints. Electrospray ionization tandem mass spectrometry was applied to analyse the newly synthesized PLs. In the presence of stable isotope-labelled PL precursors, cultured FLS were treated with either transforming growth factor-β1 (TGF-β1), bone morphogenetic protein (BMP)-2, BMP-4, BMP-7 or insulin-like growth factor-1 (IGF-1) alone or in combination with specific inhibitors of cell signalling pathways...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038523/angiotensin-ii-type-2-receptor-at2r-as-a-novel-modulator-of-inflammation-in-rheumatoid-arthritis-synovium
#14
Riccardo Terenzi, Mirko Manetti, Irene Rosa, Eloisa Romano, Felice Galluccio, Serena Guiducci, Lidia Ibba-Manneschi, Marco Matucci-Cerinic
Despite increasing evidence suggesting that angiotensin II type 2 receptor (AT2R) may regulate tissue inflammation, no study has yet analyzed its possible implication in rheumatoid arthritis (RA) synovitis. In this study, we investigated the expression and function of AT2R in synovial tissue and cultured fibroblast-like synoviocytes (FLS) from RA patients. AT2R expression was strongly increased in RA compared with osteoarthritis (OA) synovium, as well as in in cultured RA-FLS respect to OA-FLS and healthy FLS...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29029510/adenovirus-mediated-delivery-of-sema3a-alleviates-rheumatoid-arthritis-in-a-serum-transfer-induced-mouse-model
#15
Yue Teng, Zhanhai Yin, Jing Li, Kun Li, Xu Li, Yan Zhang
Rheumatoid arthritis is a chronic autoimmune disease characterized by infiltration of inflammatory cells into the synovium and destruction of cartilage and bone. Macrophages, fibroblast-like synoviocytes (FLS), and osteoclasts are critical cells driving the pathogenesis of RA. Semaphorin 3A (Sema3A) is recently identified as an essential player in the bone homeostasis, however its role in RA progression especially in the macrophage polarization are poorly understood. In the present study, we found that Sems3A levels were significantly decreased in RA serum and synovial fluid compared to OA controls...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29016475/relationship-between-physiological-off-ice-testing-on-ice-skating-and-game-performance-in-division-i-women-s-ice-hockey-players
#16
Michelle Boland, Emily M Miele, Katie Delude
The purpose was to identify off-ice testing variables that correlate to skating and game performance in Division I collegiate women ice hockey players. Twenty female, forward and defensive players (19.95 ± 1.35 yr) were assessed for weight, height, percent fat mass (%FAT), bone mineral density, predicted one repetition maximum (RM) absolute and relative (REL%) bench press (BP) and hex bar deadlift (HDL), lower body explosive power, anaerobic power, countermovement vertical jump (CMJ), maximum inspiratory pressure (MIP), and on-ice repeated skate sprint (RSS) performance...
October 7, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28987940/microrna-29a-inhibits-proliferation-and-induces-apoptosis-in-rheumatoid-arthritis-fibroblast-like-synoviocytes-by-repressing-stat3
#17
Jinxiang Liu, Dan Fei, Jie Xing, Juan Du
Rheumatoid arthritis-fibroblast-like synoviocytes (RA-FLS) with aberrant expression of microRNA (miRNA) have been reported to be involved in the initiation, progression, and perpetuation of rheumatoid arthritis (RA). In this study, we explored the biological function and underlying mechanism of microRNA-29a (miR-29a) in cultured RA-FLS from RA patients. The expression of miR-29a in serum, synovial tissues, and FLS from RA patients and health donors was detected by real-time quantitative RT-PCR (qRT-PCR). The effects of miR-29a on cell proliferation, apoptosis, and inflammatory cytokine levels in RA-FLS were also determined using Counting Assay Kit-8 (CCK-8), flow cytometry, and enzyme-linked immunosorbent assay (ELISA) respectively...
October 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28981555/platelets-contribute-to-the-accumulation-of-matrix-metalloproteinase-type-2-in-synovial-fluid-in-osteoarthritis
#18
Alessia Alunno, Emanuela Falcinelli, Filippo Luccioli, Eleonora Petito, Elena Bartoloni, Stefania Momi, Giulia Mirabelli, Giovanni B Mancini, Roberto Gerli, Paolo Gresele
Inflammation plays a role in the initiation and progression of osteoarthritis (OA), a chronic degenerative joint disorder. Platelets are inflammatory cells, contain and release matrix metalloproteinases (MMPs) and favour the release of these enzymes, key effectors of cartilage and subchondral bone degradation, by other cells; however, their role in OA has not been investigated yet. Our aims were (1) to assess the presence of platelets and of MMP-2 in synovial fluid (SF) of OA patients; (2) to evaluate the contribution of platelets to MMP-2 release by fibroblast-like synoviocytes (FLS); and (3) to investigate if hyaluronic acid (HA) interferes with these processes...
October 4, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28975975/mir-20a-regulates-fibroblast-like-synoviocyte-proliferation-and-apoptosis-in-rheumatoid-arthritis
#19
X-J Wei, X-W Li, J-L Lu, Z-X Long, J-Q Liang, S-B Wei, C-X Lu, W-Z Lu
OBJECTIVE: STAT3 expression is elevated in the synovial tissue of patients with rheumatoid arthritis (RA). MiR-20a plays a role in mediating synovial inflammation in RA. Bioinformatics analysis has identified a binding site between miR-20 and the 3'-UTR of STAT3 mRNA. This study aimed to investigate the role of miR-20a in the regulation of STAT3 expression and synovial cell proliferation as well as apoptosis. PATIENTS AND METHODS: Synovial tissues were collected from RA patients and osteoarthritis (OA) patients to measure miR-20a, STAT3, p-STAT3, and Ki-67 expressions...
October 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28973700/hematologic-malignancies-discovered-on-investigation-of-breast-abnormalities
#20
Alaa Alsadi, Dianna Lin, Hussein Alnajar, Arlen Brickman, Colin Martyn, Paolo Gattuso
OBJECTIVES: Hematological malignancies of the breast share a presentation similar to primary breast carcinomas but differ substantially in therapeutic approach and clinical outcomes. In this study, we investigate the frequency of hematological malignancies, their relative primary and secondary occurrences, and further characterize the distinct histopathologies of these malignancies with a special focus on lymphomas. To our knowledge this is one of the largest and most comprehensive studies of breast hematologic malignancies...
October 2017: Southern Medical Journal
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