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https://www.readbyqxmd.com/read/27936399/gastrodia-elata-attenuates-inflammatory-response-by-inhibiting-the-nf-%C3%AE%C2%BAb-pathway-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#1
Yu Li, Li-Min Wang, Jian-Zhong Xu, Ke Tian, Chen-Xi Gu, Zhi-Fu Li
Gastrodia elata (GE), which belongs to the Orchidaceae family, was found to possess anti-inflammatory activity. However, the effect of GE on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) remains largely unknown. Thus, the aim of this study was to investigate the effects of GE on tumor necrosis factor-α (TNF-α)-induced inflammatory response in RA-FLS and the underlying molecular mechanism was also explored. Our results demonstrated that GE significantly attenuated TNF-α-induced IL-6 and IL-8 production in RA-FLS...
December 6, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27928664/laparoscopic-skill-assessment-of-practicing-surgeons-prior-to-enrollment-in-a-surgical-trial-of-a-new-laparoscopic-procedure
#2
Benjamin Zendejas, James W Jakub, Alicia M Terando, Amod Sarnaik, Charlotte E Ariyan, Mark B Faries, Sabino Zani, Heather B Neuman, Nabil Wasif, Jeffrey M Farma, Bruce J Averbook, Karl Y Bilimoria, Douglas Tyler, Mary Sue Brady, David R Farley
BACKGROUND: Outcomes of surgical trials hinge on surgeon selection and their underlying expertise. Assessment of expertise is paramount. We investigated whether surgeons' performance measured by the fundamentals of laparoscopic surgery (FLS) assessment program could predict their performance in a surgical trial. METHODS: As part of a prospective multi-institutional study of minimally invasive inguinal lymphadenectomy (MILND) for melanoma, surgical oncologists with no prior MILND experience underwent pre-trial FLS assessment...
December 7, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27908500/effectiveness-of-a-comprehensive-mental-skills-curriculum-in-enhancing-surgical-performance-results-of-a-randomized-controlled-trial
#3
Dimitrios Stefanidis, Nicholas E Anton, Lisa D Howley, Eric Bean, Ashley Yurco, Manuel E Pimentel, Cameron K Davis
INTRODUCTION: We hypothesized that the implementation of a novel mental skills curriculum (MSC) during laparoscopic simulator training would improve mental skills and performance, and decrease stress. METHODS: Sixty volunteer novices were randomized into intervention and control groups. All participants received FLS training while the intervention group also participated in the MSC. Skill transfer and retention were assessed on a live porcine model after training and 2 months later, respectively...
November 22, 2016: American Journal of Surgery
https://www.readbyqxmd.com/read/27908299/the-infrapatellar-fat-pad-induces-inflammatory-and-degradative-effects-in-articular-cells-but-not-through-leptin-or-adiponectin
#4
Jean-Baptiste Gross, Cecile Guillaume, Pascale Gegout-Pottie, Pascal Reboul, Jean-Yves Jouzeau, Didier Mainard, Nathalie Presle
OBJECTIVES: Based on a novel approach suggesting a role of adipose tissue in osteoarthritis (OA), we aimed to determine whether the infrapatellar fat pad (IFP) may affect joint cell functions through adipokines. METHODS: The conditioned media of IFP and subcutaneous adipose tissue from OA patients were used to determine the production of leptin and adiponectin, and to stimulate chondrocytes and fibroblast-like synoviocytes (FLS). Blocking experiments were carried out to evaluate the contribution of adipokines to IFP effects...
November 13, 2016: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/27903245/the-predictive-value-of-sonographic-images-of-follicular-lesions-a-comparison-with-nodules-unequivocal-in-fna-single-centre-prospective-study
#5
Dorota Słowińska-Klencka, Martyna Wojtaszek-Nowicka, Stanisław Sporny, Ewa Woźniak-Oseła, Bożena Popowicz, Mariusz Klencki
BACKGROUND: To determine the diagnostic efficacy of ultrasonographic malignancy risk features (UMRFs) in follicular lesions (FL) in a population with low risk of malignancy in FL and to compare it with a similar analysis in a group of patients with unequivocal cytology (UC): benign lesion (BL) or malignant neoplasm (MN). METHODS: Presence of UMRFs (hypoechogenicity, solid echostructure, taller-than-wide shape, pathological vascularization, irregular margins, microcalcifications and macrocalcifications) and their sets were assessed in 322 FL: 202 follicular lesions of undetermined significance (FLUS) and 120 suspicious for follicular neoplasm (SFN) and 300 nodules with UC: 200 BL and 100 MN, subsequently evaluated histopathologically...
December 1, 2016: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/27894399/effect-of-iguratimod-and-methotrexate-on-rankl-and-opg-expression-in-serum-and-il-1%C3%AE-induced-fibroblast-like-synoviocytes-from-patients-with-rheumatoid-arthritis
#6
X T Wang, P Li, T S Xu, R Ding, X Zhang, L Q Bi
The receptor activator of nuclear factor κB ligand (RANKL)/receptor activator of nuclear factor κB (RANK)/osteoprotegerin (OPG) system plays a key role in rheumatoid arthritis (RA)-associated bone erosion. The upregulation of the RANKL/OPG ratio promotes bone erosion. The objective of this study is to explore the effects of iguratimod, a small-molecule disease-modifying antirheumatic drug (DMARD), alone or in combination with methotrexate (MTX), on RANKL and OPG expression in RA. We performed an enzyme-linked immunosorbent assay (ELISA) to investigate the modulatory effects of iguratimod, MTX, or their combination on serum RANKL and OPG levels of patients with RA before and after treatment for 12 and 24 weeks...
October 31, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27891080/enhanced-burst-suppression-and-disruption-of-local-field-potential-synchrony-in-a-mouse-model-of-focal-cortical-dysplasia-exhibiting-spike-wave-seizures
#7
Anthony J Williams, Chen Zhou, Qian-Quan Sun
Focal cortical dysplasias (FCDs) are a common cause of brain seizures and are often associated with intractable epilepsy. Here we evaluated aberrant brain neurophysiology in an in vivo mouse model of FCD induced by neonatal freeze lesions (FLs) to the right cortical hemisphere (near S1). Linear multi-electrode arrays were used to record extracellular potentials from cortical and subcortical brain regions near the FL in anesthetized mice (5-13 months old) followed by 24 h cortical electroencephalogram (EEG) recordings...
2016: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/27886945/implementation-of-models-of-care-for-secondary-osteoporotic-fracture-prevention-and-orthogeriatric-models-of-care-for-osteoporotic-hip-fracture
#8
REVIEW
Paul Mitchell, Kristina Åkesson, Manju Chandran, Cyrus Cooper, Kirtan Ganda, Muriel Schneider
As the world's population ages, the prevalence of osteoporosis and its resultant fragility fractures is set to increase dramatically. This chapter focuses on current frameworks and major initiatives related to the implementation of fracture liaison services (FLS) and orthogeriatrics services (OGS), Models of Care designed to reliably implement secondary fracture prevention measures for individuals presenting to health services with fragility fractures. The current evidence base regarding the impact and effectiveness of FLS and OGS is also considered...
June 2016: Best Practice & Research. Clinical Rheumatology
https://www.readbyqxmd.com/read/27881147/p53-predominantly-regulates-il-6-production-and-suppresses-synovial-inflammation-in-fibroblast-like-synoviocytes-and-adjuvant-induced-arthritis
#9
Ting Zhang, Huihua Li, Juan Shi, Sha Li, Muyuan Li, Lei Zhang, Leting Zheng, Dexian Zheng, Fulin Tang, Xuan Zhang, Fengchun Zhang, Xin You
BACKGROUND: Dominant-negative somatic mutations of p53 has been identified in the synovium of patients with rheumatoid arthritis (RA), in which interleukin (IL)-6 has been established as a pivotal inflammatory cytokine. The aim of this study was to clarify the significance of p53 in the longstanding inflammation in RA by modulating IL-6. METHODS: We established adjuvant-induced arthritis (AIA) in Lewis rats and treated them with p53 activator, and then analyzed the histopathology of the synovium and IL-6 expression...
November 24, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27866531/-effect-of-10-hydroxycamptotbecine-on-the-proliferation-of-human-fibroblast-like-synoviocyte-with-rheumatoid-arthritis
#10
L L Ren, X J Lu, J X Shou, Y Wang, X L Sun, L J Zhang, M Zhang, Y Meng, H Sun
Objective: To study the effect of 10-Hydroxycamptothecine (10-HCPT) on the proliferation and apoptosis of human Fibroblast-like Synoviocyte (FLS) with Rheumatoid Arthritis (RA). Methods: Different concentrations of 10-HCPT and Methotrexate (MTX) were used to treat FLS cells in RA and Osteoarthritis (OA) for different time (24, 48, and 72 hours), and FLS cells without 10-HCPT and MTX were served as the control group. CCK-8 assay were applied to determine the proliferation of FLS cells, Annexin-V APC/7-AAD staining were used to detect the apoptosis of FLS cells...
November 15, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27860244/role-of-protein-arginine-methyltransferase-5-in-inflammation-and-migration-of-fibroblast-like-synoviocytes-in-rheumatoid-arthritis
#11
Dongying Chen, Shan Zeng, Mingcheng Huang, Hanshi Xu, Liuqin Liang, Xiuyan Yang
To probe the role of protein arginine methyltransferase 5 (PRMT5) in regulating inflammation, cell proliferation, migration and invasion of fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). FLSs were separated from synovial tissues (STs) from patients with RA and osteoarthritis (OA). An inhibitor of PRMT5 (EPZ015666) and short interference RNA (siRNA) against PRMT5 were used to inhibit PRMT5 expression. The standard of protein was measured by Western blot or immunofluorescence...
November 17, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27857238/implementation-of-fracture-liaison-service-in-a-new-zealand-public-hospital-waitemata-district-health-board-experience
#12
David Kim, Denise Mackenzie, Rick Cutfield
AIM: To analyse the performance of a Fracture Liaison Service (FLS) at Waitemata District Health Board (WDHB), and to detail how systematic secondary fracture prevention can be delivered in a secondary healthcare setting in New Zealand. METHOD: Clinical details of patients supervised by the WDHB FLS during the calendar year 2014 were reviewed and analysed. Additional information including treatment compliance and re-fracture rates were sought a year after initial intervention...
November 18, 2016: New Zealand Medical Journal
https://www.readbyqxmd.com/read/27856663/stable-activation-of-fibroblasts-in-rheumatic-arthritis-causes-and-consequences
#13
REVIEW
Adelheid Korb-Pap, Jessica Bertrand, Joanna Sherwood, Thomas Pap
The progressive destruction of articular cartilage is a hallmark of RA, a systemic autoimmune disease predominantly affecting synovial joints that often results in severe disability. Fibroblast-like synoviocytes (FLSs) have been demonstrated to play a key role in both the initiation and perpetuation of the disease. During RA pathogenesis, FLSs acquire a permanently aggressive, tumour-like phenotype that mediates cartilage destruction both directly and indirectly. This short review summarizes the recent advances in the understanding of FLS cellular transformation during RA, as well as the consequences for disease progression and for novel treatment strategies...
December 2016: Rheumatology
https://www.readbyqxmd.com/read/27853259/comparative-transcriptome-analysis-of-chinary-assamica-and-cambod-tea-camellia-sinensis-types-during-development-and-seasonal-variation-using-rna-seq-technology
#14
Ajay Kumar, Vandna Chawla, Eshita Sharma, Pallavi Mahajan, Ravi Shankar, Sudesh Kumar Yadav
Tea quality and yield is influenced by various factors including developmental tissue, seasonal variation and cultivar type. Here, the molecular basis of these factors was investigated in three tea cultivars namely, Him Sphurti (H), TV23 (T), and UPASI-9 (U) using RNA-seq. Seasonal variation in these cultivars was studied during active (A), mid-dormant (MD), dormant (D) and mid-active (MA) stages in two developmental tissues viz. young and old leaf. Development appears to affect gene expression more than the seasonal variation and cultivar types...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27849385/fracture-liaison-services-multidisciplinary-approaches-to-secondary-fracture-prevention
#15
Patricia Mejia Osuna, Mary D Ruppe, Laila S Tabatabai
CONTEXT: A well-recognized gap exists between evidence-based recommendations for post-fracture care and actual clinical practice, demonstrated by the high percentage of fragility fracture patients who are neither diagnosed nor treated for osteoporosis. Our purpose is to review fracture liaison service (FLS) models and to evaluate national and international experiences in secondary fracture prevention. EVIDENCE ACQUISITION: We performed a systematic search of publication databases (MEDLINE, SCOPUS) and included randomized controlled trials, meta-analyses and review articles using the following keywords: Fracture liaison services, Secondary prevention of fracture, Post-fracture health care gap and Fragility fractures...
November 16, 2016: Endocrine Practice
https://www.readbyqxmd.com/read/27847407/anti-inflammatory-effects-of-traf-interacting-protein-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#16
Qing-Zhu Kong, Li-Tao Guo, Jia-Ning Yang, Yan-Fei Wang, Jing-Xin Zhao, Su-Hong Kong, Meng Zhang, Shi Yan, Yu Jin
Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease characterized by inflammatory cell infiltration, synovial inflammation, and cartilage destruction. Proliferative fibroblast-like synoviocytes (FLS) play crucial roles in both propagation of inflammation and joint damage because of their production of great amount of proinflammatory cytokines and proteolytic enzymes. In this study, we investigate the role of TRAF-interacting protein (TRIP) in regulating inflammatory process in RA-FLS. TRIP expression was attenuated in RA-FLS compared with osteoarthritis- (OA-) FLS...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27836511/deep-sequencing-of-the-transcriptome-reveals-distinct-flavonoid-metabolism-features-of-black-tartary-buckwheat-fagopyrum-tataricum-garetn
#17
REVIEW
Huipeng Yao, Chenglei Li, Haixia Zhao, Jianlan Zhao, Hui Chen, Tongliang Bu, Wang Anhu, Qi Wu
Black tartary buckwheat is recognized as 'black pearl' because of containg more rutin and other flavonoids as compared to yellow tartary buckwheat (traditional tartary buckwheat). Here, we show a genome-wide comparison of their transcriptomes by using an RNA-seq approach to elucidate the different molecular metabolism on the flowers from Black tartary buckwheat (HEIFENG No1) and yellow tartary buckwheat (XIQIAO No2). Over 48.4 million paired-end reads were assembled into 57,800 unigenes, of which about 57.9% (33, 472 unigenes) were annotated by BLAST searches in the NCBI non-redundant protein database...
November 8, 2016: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/27824863/demethylation-of-microrna-124a-genes-attenuated-proliferation-of-rheumatoid-arthritis-derived-fibroblast-like-synoviocytes-and-synthesis-of-tumor-necrosis-factor-%C3%AE
#18
Qiao Zhou, Li Long, Ting Zhou, Juan Tian, Bin Zhou
OBJECTIVE: To examine the impact of 5-Aza-2'-deoxycytidine (5-AzadC) on methylation status of miR-124a genes in rheumatoid arthritis (RA) associated fibroblast-like synoviocytes (FLS) and its effect on RA-FLS proliferation and TNF-α expression. MATERIALS AND METHODS: FLS were isolated from seven RA-derived synovial tissues and cultured in vitro. The expression of miR-124a was measured by real time quantitative polymerase chain reaction (PCR) in FLS with or without 5-AzadC treatment...
2016: PloS One
https://www.readbyqxmd.com/read/27824387/fler-utreddes-f%C3%A3-r-osteoporos-efter-inf%C3%A3-rande-av-frakturkedja
#19
Michael Lovric, Eva Ribom-Bornefalk
The aim of this retrospective study was to review the effect of a fracture liaison service (FLS) in terms of assessment and treatment of osteoporosis. An FLS is a model of care for improving the secondary preventive care of these patients. An FLS was started at Halland Hospital Halmstad in October 2013. In this study we evaluated the effect of the FLS on the follow up of patients with wrist fractures. In total 126 patients were included, equally divided in two groups before and after the implementation of the FLS...
November 1, 2016: Läkartidningen
https://www.readbyqxmd.com/read/27766627/whole-body-magnetic-resonance-imaging-in-newly-diagnosed-multiple-myeloma-early-changes-in-lesional-signal-fat-fraction-predict-disease-response
#20
Arash Latifoltojar, Margaret Hall-Craggs, Neil Rabin, Rakesh Popat, Alan Bainbridge, Nikolaos Dikaios, Magdalena Sokolska, Ali Rismani, Shirley D'Sa, Shonit Punwani, Kwee Yong
Cross-sectional imaging techniques are being increasingly used for disease evaluation in patients with multiple myeloma. Whole body magnetic resonance imaging (WB-MRI) scanning is superior to plain radiography in baseline assessment of patients but changes following treatment have not been systematically explored. We carried out paired WB-MRI scans in 21 newly diagnosed patients prior to, and 8-weeks after, starting chemotherapy, and analysed stringently selected focal lesions (FLs) for parametric changes. A total of 323 FLs were evaluated, median 20 per patient...
October 21, 2016: British Journal of Haematology
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