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https://www.readbyqxmd.com/read/29717774/physcion-8%C3%A2-o%C3%A2-%C3%AE-%C3%A2-glucopyranoside-extracted-from-polygonum-cuspidatum-exhibits-anti%C3%A2-proliferative-and-anti%C3%A2-inflammatory-effects-on-mh7a-rheumatoid-arthritis%C3%A2-derived-fibroblast%C3%A2-like-synoviocytes-through-the-tgf%C3%A2-%C3%AE-mapk-pathway
#1
Qin Geng, Qiaofeng Wei, Shujun Wang, Huili Qi, Qin Zhu, Xia Liu, Xiaojun Shi, Shuyun Wen
The present study aimed to investigate the anti‑arthritic effect of physcion 8‑O‑β‑glucopyranoside (POGD) and its possible mechanisms. The anti‑proliferative effects of POGD on MH7A cells were detected using a CCK‑8 assay, and the release of pro‑inflammatory cytokines, interleukin (IL)‑1β, IL‑6, IL‑8, IL‑12 and IL‑17A, were determined by ELISA. A type II collagen‑induced arthritis (CIA) rat model was established to evaluate the anti‑arthritic effect of POGD in vivo. The paw volumes, arthritis indices and serum levels of tumor necrosis factor (TNF)‑α, IL‑1β, IL‑6, IL‑8, IL‑17A were determined by ELISA...
April 30, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29717129/gata4-regulates-angiogenesis-and-persistence-of-inflammation-in-rheumatoid-arthritis
#2
Wanwan Jia, Weijun Wu, Di Yang, Chenxi Xiao, Mengwei Huang, Fen Long, Zhenghua Su, Ming Qin, Xinhua Liu, Yi Zhun Zhu
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by abnormal inflammation, angiogenesis, and cartilage destruction. In RA, neoangiogenesis is an early and crucial event to promote the formation of pannus, causing further inflammatory cell infiltration. The transcription factor GATA4 is a critical regulator of cardiac differentiation-specific gene expression. We find that a higher level of GATA4 exists in synovium of rheumatoid arthritis (RA) patients, but the function of GATA4 in RA remains unclear...
May 2, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29690734/-effect-of-dishevelled-2-on-apoptosis-in-rheumatoid-arthritis-fibroblast-like-synoviocytes-through-inhibiting-jak-stat-pathway
#3
X Z Liu, Y Gao, C H Zhu, K Qi, X Xu, D B Zhao
Objective: To investigate if Dishevelled 2 (DVL2) regulates the apoptosis of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) via the JAK-STAT pathway. Methods: DVL2 overexpressed lentivirus was transfected into RA-FLS and the apoptosis rate was detected by flow cytometry. The effect of DVL2 on RA-FLS signaling pathway was detected by RNA-seq, and then the key genes were verified by RT-PCR. Results: Compared with the control group, DVL2 significantly increased the apoptosis rate of MH7A (3.2%±2.2% vs 25...
April 17, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29581736/mir-143-3p-regulates-cell-proliferation-and-apoptosis-by-targeting-igf1r-and-igfbp5-and-regulating-the-ras-p38-mapk-signaling-pathway-in-rheumatoid-arthritis
#4
Zhenguo Yang, Jifu Wang, Zhuangzhuang Pan, Yihang Zhang
It has been demonstrated that the deregulation of microRNAs (miRNAs) affects the development of rheumatoid arthritis (RA). The primary objective of the current study was to determine the role of miR-143-3p in the progression of RA. The expression of miR-143-3p in synovium taken from patients with RA was assessed by reverse transcription-quantitative polymerase chain reaction. The expression of miR-143-3p was higher in synovium tissues of RA than that of osteoarthritis (OA). The decreased expression of miR-143-3p suppressed cell proliferation and promoted apoptosis in vitro ...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29467860/roles-of-microrna-539-and-osteopontin-in-rheumatoid-arthritis
#5
Yan Liu, Kai Qian, Chunmei Li, Yi Ma, Xingguo Chen
The present study aimed to investigate the role and mechanism of microRNA-539 (miR-539) in rheumatoid arthritis (RA). A total of 68 RA patients and 46 osteoarthritis patients were enrolled into the current study. Peripheral blood and joint fluid were collected prior to treatment. Reverse transcription-quantitative polymerase chain reaction was performed to detect osteopontin (OPN) mRNA and miR-539 expression levels, while ELISA and western blot analysis were applied to detect OPN protein expression. In addition, bioinformatics analysis predicted that miR-539 directly targeted OPN, while dual-luciferase assay was used to validate this finding...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456809/hypericin-photodynamic-therapy-inhibits-proliferation-and-induces-apoptosis-in-human-rheumatoid-arthritis-fibroblast-like-synoviocytes-cell-line-mh7a
#6
Kun Zhang, Shan Gao, JiaYi Guo, GuoHua Ni, Zhe Chen, Feng Li, XiaoLei Zhu, YongBing Wen, YanXing Guo
Objectives: To elucidate the effects and potential mechanisms of hypericin-photodynamic therapy (HYP-PDT) for treating the human rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) MH7A cell-line. Materials and Methods: MH7A cells were subjected to HYP-PDT intervention and apoptosis was evaluated via MTT, nuclear staining, and flowcytometry analyses. Intracellular reactive oxygen species (ROS) were measured with the fluorescent probe 2'7'-dichlorofluorescein diacetate (DCFH-DA)...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29385909/wu-tou-decoction-inhibits-angiogenesis-in-experimental-arthritis-by-targeting-vegfr2-signaling-pathway
#7
Lianhua He, Chunfang Liu, Congcong Sun, Jingxia Wang, Kai Zhi, Danni Sun, Hui Wang, Qianqian Wang, Na Lin
Wu-tou decoction (WTD) is a classic traditional Chinese medicine formula and has been extensively used for the treatment of rheumatoid arthritis (RA). Previous reports indicate that WTD possesses anti-inflammatory and antinociceptive activities, and inhibits the development of arthritic joints and disease severity of collagen-induced arthritis (CIA) or adjuvant-induced rats; however, its action on angiogenesis of RA has not been clarified. This study aims to determine the anti-angiogenic activity of WTD in CIA rats and in various angiogenesis models...
March 19, 2018: Rejuvenation Research
https://www.readbyqxmd.com/read/29210647/%C3%AE-mangostin-a-natural-xanthone-induces-apoptosis-and-ros-accumulation-in-human-rheumatoid-fibroblast-like-synoviocyte-mh7a-cells
#8
J Zhang, Q Chen, S Wang, T Li, Z Xiao, W Lan, G Huang, X Cai
BACKGROUND: Rheumatoid arthritis (RA) is a complicated and heterogeneous chronic disease with the characteristic of progressive joint destruction, deformity and disability. It is associated with not only genetic but also environmental factors. Many studies suggest that RA-derived fibroblast-like synoviocyte (RA-FLS) is involved in the pathogenic process of RA. The apoptosis and proliferation of RA-FLS is of great importance in the development and progression in RA. Nowadays, more and more traditional Chinese medicine (TCM) or natural products are studied in the treatment of RA...
2017: Current Molecular Medicine
https://www.readbyqxmd.com/read/29083085/cdk7-inhibition-suppresses-rheumatoid-arthritis-inflammation-via-blockage-of-nf-%C3%AE%C2%BAb-activation-and-il-1%C3%AE-il-6-secretion
#9
Honghai Hong, Yingmin Zeng, Wenxuan Jian, Lei Li, Liying Lin, Yousheng Mo, Meiling Liu, Shuhuan Fang, Yong Xia
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by joint swelling, joint tenderness and destruction of synovial joints, leading to severe disability. Anti-inflammatory drugs and disease-modifying anti-rheumatic drugs (DMARDs) may improve RA process. However, in most patients the treatment effect is still not satisfactory. Cyclin-dependent kinase 7 (CDK7) plays a well-established role in the regulation of the eukaryotic cell division cycle, and recent studies indicated that it exerted anti-inflammatory effect...
February 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29070455/-efficacy-of-internalized-rgd-modified-echogenic-liposomes-in-diagnosis-and-treatment-in-a-mouse-model-of-rheumatoid-arthritis
#10
Zhen Gan, Hao Wu, Hao-Han Wu, Mei-Jun Zhou, Fei Yan, Hong-Mei Liu
OBJECTIVE: To prepare internalized RGD (iRGD) modified echogenic liposomes containing methotrexate (MTX) and indocyanine green (ICG) (iRGD MTX ICG ELIP) and evaluate its targeting efficiency and inhibitory effect combined with ultrasound on synovial cells. METHODS: iRGD MTX ICG ELIP was prepared by the thin film rehydration and freeze-lyophilization method and its general characteristics and acoustic responsiveness were assessed. The targeting effect of the prepared liposome was observed by assessing its cell uptake in vitro...
October 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28779958/efficacy-of-indocyanine-green-mediated-sonodynamic-therapy-on-rheumatoid-arthritis-fibroblast-like-synoviocytes
#11
Qin Tang, Shufang Chang, Zhonghua Tian, Jiangchuan Sun, Lan Hao, Zhigang Wang, Shenyin Zhu
Sonodynamic therapy (SDT) has become a new therapeutic method because of its activation of certain sensitizers by ultrasound. Some studies have reported that indocyanine green (ICG) has the characteristics of a sonosensitizer and favorable fluorescence imaging in synovitis of early inflammatory arthritis. In this study, we aimed to investigate the cytotoxic effect of ICG-mediated SDT on MH7A cells in vitro and the potential mechanisms involved. ICG was found to be taken up mainly in cytoplasm, with maximal uptake in 4 h...
August 2, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28744810/kr%C3%A3-ppel-like-factor-4-regulates-the-expression-of-inducible-nitric-oxide-synthase-induced-by-tnf-%C3%AE-in-human-fibroblast-like-synoviocyte-mh7a-cells
#12
Xuanrong Mo, Jie Chen, Xinjuan Wang, Zhenyu Pan, Yuping Ke, Zhidong Zhou, Jiangwen Xie, Guoju Lv, Xinjing Luo
Krüppel-like factor 4 (KLF4), a zinc finger transcription factor, has been implicated in the inflammation mediated by macrophages and endothelial cells by regulating the expression of inflammatory mediators. Here, we investigated whether KLF4 affects the expression of inducible nitric oxide synthase (iNOS), an important inflammatory mediator, in the human RA fibroblast-like synovial cell line MH7A. A pcDNA3.1-KLF4 plasmid or short interfering RNA KLF4 was transfected into MH7A cells, and the iNOS expression and nitric oxide (NO) production were analyzed by quantitative PCR, immunoblotting, and nitrite measurement...
January 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28743324/atomic-force-microscopy-study-of-the-anti-inflammatory-effects-of-triptolide-on-rheumatoid-arthritis-fibroblast-like-synoviocytes
#13
Zhanhui Su, Han Sun, Man Ao, Chunying Zhao
High-resolution atomic force microscopy (AFM) was used for the in situ evaluation of the anti-inflammatory effects of triptolide on rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) to understand the anti-RA effects of triptolide, based on the morphological and biophysical changes observed in RA-FLS. RA-FLS have been reported to play a primary role in inflammatory bone destruction during the development of RA and thus are regarded as an important target for RA treatment. Triptolide pretreatment significantly inhibited tumor necrosis factor-α-induced expression of the interleukin (IL)-1β, IL-6, and IL-8 genes in MH7A cells...
October 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28682648/kinase-inhibitors-of-the-igf-1r-as-a-potential-therapeutic-agent-for-rheumatoid-arthritis
#14
Hiroshi Tsushima, Shinji Morimoto, Maki Fujishiro, Yuko Yoshida, Kunihiro Hayakawa, Takuya Hirai, Tomoko Miyashita, Keigo Ikeda, Ken Yamaji, Kenji Takamori, Yoshinari Takasaki, Iwao Sekigawa, Naoto Tamura
We have previously shown that the inhibition of connective tissue growth factor (CTGF) is a potential therapeutic strategy against rheumatoid arthritis (RA). CTGF consists of four distinct modules, including the insulin-like growth factor binding protein (IGFBP). In serum, insulin-like growth factors (IGFs) bind IGFBPs, interact with the IGF-1 receptor (IGF-1 R), and regulate anabolic effects and bone metabolism. We investigated the correlation between IGF-1 and the pathogenesis of RA, and the inhibitory effect on osteoclastogenesis and angiogenesis of the small molecular weight kinase inhibitor of the IGF-1 R, NVP-AEW541, against pathogenesis of RA in vitro...
July 6, 2017: Autoimmunity
https://www.readbyqxmd.com/read/28586015/evaluation-of-the-anti-inflammatory-actions-of-various-functional-food-materials-including-glucosamine-on-synovial-cells
#15
Yoshie Yamagishi, Akimasa Someya, Kensuke Imai, Junji Nagao, Isao Nagaoka
The anti-inflammatory actions of glucosamine (GlcN) on arthritic disorders involve the suppression of inflammatory mediator production from synovial cells. GlcN has also been reported to inhibit the activation of the p38 mitogen-activated protein kinase (MAPK) pathway. The present study aimed to determine the cooperative and anti‑inflammatory actions of functional food materials and evaluated the production of interleukin (IL)‑8 and phosphorylation of p38 MAPK in IL-1β-activated synovial cells, incubated with the combination of GlcN and various functional food materials containing L‑methionine (Met), undenatured type II collagen (UC‑II), chondroitin sulfate (CS), methylsulfonylmethane (MSM) and agaro-oligosaccharide (AO)...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28487948/effect-of-aloe%C3%A2-emodin-on-the-proliferation-and-apoptosis-of-human-synovial-mh7a-cells-a-comparison-with-methotrexate
#16
Motoko Hashiguchi, Kaori Suzuki, Kazuo Kaneko, Isao Nagaoka
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by synovial hyperplasia. Methotrexate (MTX), an antifolate derivative, is used for the treatment of RA, as it exerts antiproliferative efftects on lymphocytes and synovial cells. Aloe‑emodin (AE) is a primary component of anthraquinones in Aloe vera and exerts antiproliferative and apoptotic effects on various tumor cells. In the present study, the effect of AE on the proliferation and apoptosis of MH7A human RA synovial cells was examined...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28383556/synovial-cell-death-is-regulated-by-tnf-%C3%AE-induced-expression-of-b-cell-activating-factor-through-an-erk-dependent-increase-in-hypoxia-inducible-factor-1%C3%AE
#17
Jae-Wook Lee, Jiyoung Lee, Sung Hee Um, Eun-Yi Moon
B-cell activating factor (BAFF) has a role in the maturation and maintenance of B cells and is associated with rheumatoid arthritis (RA). Here, we investigated whether tumor necrosis factor (TNF)-α-induced BAFF expression controls the survival of fibroblast-like synoviocytes (FLS) and whether their survival can be regulated by TNF-α-mediated upregulation of hypoxia-inducible factor (HIF)-1α using MH7A synovial cells transfected with the SV40 T antigen. More TNF-α-treated cells died compared with the control...
April 6, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28314444/pdk1-promotes-the-inflammatory-progress-of-fibroblast-like-synoviocytes-by-phosphorylating-rsk2
#18
Chi Sun, Yu Sun, Dingjun Jiang, Guofeng Bao, Xinhui Zhu, Dawei Xu, Youhua Wang, Zhiming Cui
This study investigated the role of PDK1 in inflammatory response which is initiated by TNF-α and analyzed the association between PDK1 and RSK2. TNF-α were added into MH7A cells to induce inflammation condition. Through overexpressing or suppressing PDK1 in MH7A cells, the role of PDK1 in cell invasiveness and inflammatory factors was determined. Levels of MMPs protein and inflammatory cytokines were assessed with PDK1 siRNA and TNF-α treatment. Inhibition of RSK2 was used to investigate the function of RSK2 on PDK1-induced inflammation...
May 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28123298/oxygen-and-indocyanine-green-loaded-phase-transition-nanoparticle-mediated-photo-sonodynamic-cytotoxic-effects-on-rheumatoid-arthritis-fibroblast-like-synoviocytes
#19
Qin Tang, Jianyu Cui, Zhonghua Tian, Jiangchuan Sun, Zhigang Wang, Shufang Chang, Shenyin Zhu
BACKGROUND: Photodynamic therapy and sonodynamic therapy are developing, minimally invasive, and site-specific modalities for cancer therapy. A combined strategy PSDT (photodynamic therapy followed by sonodynamic therapy) has been proposed in this study. Here, we aimed to develop novel biodegradable poly(DL-lactide-co-glycolic acid) phase-transition nanoparticles simultaneously loaded with oxygen and indocyanine green (OI-NPs) and to investigate the cytotoxic effects and the potential mechanisms of OI-NP-mediated PSDT on MH7A synoviocytes...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28120218/platelet-rich-plasma-inhibits-inflammatory-factors-and-represses-rheumatoid-fibroblast-like-synoviocytes-in-rheumatoid-arthritis
#20
Shichao Tong, Ji Liu, Changqing Zhang
Rheumatoid arthritis (RA) is a chronic disease affecting daily life of numerous patients, and uncontrolled proliferation of synovial fibroblasts plays vital role during the pathology of RA. Platelet-rich plasma (PRP), widely used in tissue regeneration and pain management, is rarely studied in RA. This study aims to investigate the effect of PRP on synovial fibroblasts during RA. Rheumatoid fibroblast-like synoviocyte MH7A cells were stimulated by lipopolysaccharide (LPS) to simulate RA conditions and treated with PRP, after that the concentration of inflammatory factors interleukin (IL) 1β, tumor necrosis factor alpha (TNFα) and IL6 in the supernatant of culture medium was quantified by ELISA...
November 2017: Clinical and Experimental Medicine
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