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https://www.readbyqxmd.com/read/27916091/-tryptase-inhibits-cell-apoptosis-through-upregulating-par-2-and-rho-kinase-in-rheumatoid-arthritis-synovial-fibroblasts
#1
Qianqian Zheng, Shigang Li, Yunli Jia, Wei Liu, Lingling Yu, Xianyong Chen, Jinling Wang
Objective To investigate the regulatory effects of tryptase on protease-activated receptors 2 (PAR-2), Rho signal pathway and apoptosis of MH7A rheumatoid arthritis synovial fibroblasts. Methods In MH7A cells, the expression of PAR-2 was measured by flow cytometry; cell apoptosis was examined by annexin V- FITC/PI staining combined with flow cytometry; the expression of Rho kinase was detected by Pull-down assay and Western botting. Results Tryptase upregulated the expression of PAR-2 in MH7A cells, and suppressed Fas-mediated apoptosis of MH7A cells in a dose-dependent manner...
December 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27776166/glucosamine-downregulates-the-il-1%C3%AE-induced-expression-of-proinflammatory-cytokine-genes-in-human-synovial-mh7a-cells-by-o-glcnac-modification-dependent-and-independent-mechanisms
#2
Akimasa Someya, Takako Ikegami, Koji Sakamoto, Isao Nagaoka
Osteoarthritis (OA) is one of the major joint diseases, and the synovial inflammation is involved in the pathogenesis and progression of OA. Glucosamine (GlcN) is widely used as a dietary supplement for OA, and is expected to exert the antiinflammatory action in OA. However, the detailed mechanism for the antiinflammatory action of GlcN remains poorly understood. In this study, to elucidate the molecular mechanism involved in the GlcN-medicated regulation of synovial cell activation, we comprehensively analyzed the effect of GlcN on the gene expression using a human synovial cell line MH7A by DNA microarray...
2016: PloS One
https://www.readbyqxmd.com/read/27763638/1-25-dihydroxy-vitamin-d3-with-tumor-necrosis-factor-alpha-protects-against-rheumatoid-arthritis-by-promoting-p53-acetylation-mediated-apoptosis-via-sirt1-in-synoviocytes
#3
Xin Gu, Bingjie Gu, Xianhui Lv, Zhenzhen Yu, Rong Wang, Xiaoli Zhou, Wanxin Qiao, Zhiyuan Mao, Guoping Zuo, Qing Li, Dengshun Miao, Jianliang Jin
Impaired apoptosis of fibroblast-like synoviocytes (FLSs) causes synovial hyperplasia, facilitating destruction of cartilage and bone in rheumatoid arthritis (RA). Tumor necrosis factor (TNF)-α, a dominant inflammatory mediator in RA pathogenesis, promotes progression of RA symptoms. Prevalence of 1, 25-dihydroxy-vitamin D3 (hereafter termed VD) deficiency is 30-63% in patients with RA. Whether VD leads to apoptosis or enhances TNF-α-mediated apoptosis in FLSs to ameliorate RA is unclear. To determine this, 10-week-old CYP27B1-deficient (CYP27B1(-/-)) mice with collagen-induced arthritis (CIA) were intraperitoneally treated with 1 μg/kg VD every other day for 9 weeks...
October 20, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27744121/s-propargyl-cysteine-attenuates-inflammatory-response-in-rheumatoid-arthritis-by-modulating-the-nrf2-are-signaling-pathway
#4
Wei-Jun Wu, Wan-Wan Jia, Xin-Hua Liu, Li-Long Pan, Qiu-Yan Zhang, Di Yang, Xiao-Yan Shen, Liang Liu, Yi Zhun Zhu
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder. Hydrogen sulfide (H2S), the third physiological gasotransmitter, is well recognized as an anti-inflammatory mediator in various inflammatory conditions. Herein, we explored the protective effects of S-propargyl-cysteine (SPRC, also known as ZYZ-802), an endogenous H2S modulator, on RA and determined the underlying mechanisms. In the present study, SPRC concentration-dependently attenuated inflammatory mediator expression, reactive oxidase species generation, and the expression and activity of matrix metalloproteinases (MMP)-9 in interleukin (IL)-1β-induced human rheumatoid fibroblast-like synoviocytes MH7A...
October 6, 2016: Redox Biology
https://www.readbyqxmd.com/read/27589701/dimacrolide-sesquiterpene-pyridine-alkaloids-from-the-stems-of-tripterygium-regelii
#5
Dongsheng Fan, Guo-Yuan Zhu, Ting Li, Zhi-Hong Jiang, Li-Ping Bai
Two new dimacrolide sesquiterpene pyridine alkaloids (DMSPAs), dimacroregelines A (1) and B (2), were isolated from the stems of Tripterygium regelii. The structures of both compounds were characterized by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data. Compounds 1 and 2 are two rare DMSPAs possessing unique 2-(3'-carboxybutyl)-3-furanoic acid units forming the second macrocyclic ring, representing the first example of DMSPAs bearing an extra furan ring in their second macrocyclic ring system...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27550416/modulation-of-il-6-induced-rankl-expression-in-arthritic-synovium-by-a-transcription-factor-sox5
#6
Xiaoke Feng, Yumeng Shi, Lingxiao Xu, Qiuyue Peng, Fang Wang, Xiaoxi Wang, Wei Sun, Yan Lu, Betty P Tsao, Miaojia Zhang, Wenfeng Tan
Receptor activator of nuclear factor κB ligand (RANKL) is critically involved in bone erosion of rheumatoid arthritis (RA). We previously reported association between younger age at onset of RA and a RANKL promoter SNP that conferred an elevated promoter activity via binding to a transcription factor SOX5. Here we study the regulation of SOX5 levels in relation to RANKL expression in RA synovial fibroblasts (SF) and the development of bone erosion in the collagen-induced arthritis (CIA) mouse. Our data indicated SOX5 levels were higher in synovium and synovial fluid from RA compared to osteoarthritis patients...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27419047/a-comparative-proteomics-study-of-a-synovial-cell-line-stimulated-with-tnf-%C3%AE
#7
Seiji Shibasaki, Miki Karasaki, Shunsuke Aburaya, Hironobu Morisaka, Yumiko Takeda, Wataru Aoki, Sachie Kitano, Masayasu Kitano, Mitsuyoshi Ueda, Hajime Sano, Tsuyoshi Iwasaki
To elucidate the pathogenesis of rheumatoid arthritis (RA), we used proteomic analysis to determine the protein profile in a synovial cell line, MH7A, established from patients with RA. Proteins were extracted from MH7A cells that were or were not stimulated with tumor necrosis factor-α (TNF-α), and then analyzed on a liquid chromatography/mass spectrometry system equipped with a unique long monolithic silica capillary. On the basis of the results of this proteomic analysis, we identified 2650 proteins from untreated MH7A cells and 2688 proteins from MH7A cells stimulated with TNF-α...
May 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27213322/anti-inflammatory-oleanolic-triterpenes-from-chinese-acorns
#8
Jie Huang, Yihai Wang, Chuan Li, Xinluan Wang, Xiangjiu He
Acorns play an important role in human history and are a source of food and recipes for many cultures around the world. In this study, eleven oleanolic triterpenes, one of which was novel, were isolated from Chinese acorns (Quercus serrata var. brevipetiolata). The chemical structure of the novel triterpene, which was identified as 2α,3β,19α-trihydroxy-24-oxo-olean-12-en-28-oic acid (1), was established based on the interpretation of chemical and spectroscopic analyses, including IR, HR-ESI-MS, and NMR experiments (¹H, (13)C NMR, DEPT, ¹H-¹H COSY, HSQC, HMBC, and NOESY)...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/26970310/metastasis-associated-protein-1-mta1-signaling-in-rheumatoid-synovium-regulation-of-inflammatory-response-and-cytokine-mediated-production-of-prostaglandin-e2-pge2
#9
Hui Wang, Bing-Wei Dong, Zhao-Hui Zheng, Zhen-Biao Wu, Wei Li, Jin Ding
Abnormal perpetual inflammatory response and sequential cytokine-induced prostaglandin E2 (PGE2) play important roles in the pathogenesis of rheumatoid arthritis (RA). The underlying regulatory mechanism, however, remain largely unknown. Here, we discovered that expression level of Metastasis associated protein 1 (MTA1), an important chromatin modifier that plays a critical role in transcriptional regulation by modifying DNA accessibility for cofactors, was upregulated in human rheumatoid synovial tissues. Furthermore, a knockdown of MTA1 by siRNA in the human fibroblast-like synovial cell line MH7A was found to impair the 4-hydroxynonenal (4-HNE)-induced transcriptional expression levels of certain proinflammatory cytokines including IL-1β, TNF-α and IL-6...
April 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26640499/shikonin-inhibits-inflammatory-response-in-rheumatoid-arthritis-synovial-fibroblasts-via-lncrna-nr024118
#10
Ke-Ya Yang, Dong-Liang Chen
Background. Shikonin is a major chemical component of zicao that possesses anti-inflammatory properties and the ability to mediate cellular and humoral immunity, especially in rheumatoid arthritis (RA). We investigated the impact of shikonin on inflammatory response in RA synovial fibroblasts using the CAIA model. Methods. Severe polyarticular arthritis was induced in Balb/c female mice. Expressions of lncRNA-NR024118, SOCS3, proinflammatory cytokines, and MMPs were evaluated using RT-RCR. Histone acetylation and SOCS3 protein expression were assessed by ChIP assay and western blot, respectively...
2015: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/26614458/tetrandrine-inhibits-migration-and-invasion-of-rheumatoid-arthritis-fibroblast-like-synoviocytes-through-down-regulating-the-expressions-of-rac1-cdc42-and-rhoa-gtpases-and-activation-of-the-pi3k-akt-and-jnk-signaling-pathways
#11
Qi Lv, Xian-Yang Zhu, Yu-Feng Xia, Yue Dai, Zhi-Feng Wei
Tetrandrine (Tet), the main active constituent of Stephania tetrandra root, has been demonstrated to alleviate adjuvant-induced arthritis in rats. The present study was designed to investigate the effects of Tet on the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) and explore the underlying mechanisms. By using cultures of primary FLS isolated from synoviums of RA patients and cell line MH7A, Tet (0.3, 1 μmol·L(-1)) was proven to significantly impede migration and invasion of RA-FLS, but not cell proliferation...
November 2015: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/26584911/inhibition-of-each-module-of-connective-tissue-growth-factor-as-a-potential-therapeutic-target-for-rheumatoid-arthritis
#12
Tomoko Miyashita, Shinji Morimoto, Maki Fujishiro, Kunihiro Hayakawa, Satoshi Suzuki, Keigo Ikeda, Keiji Miyazawa, Megumi Morioka, Kenji Takamori, Hideoki Ogawa, Iwao Sekigawa, Yoshinari Takasaki
We previously reported the importance of connective tissue growth factor (CTGF) in rheumatoid arthritis (RA). CTGF contains four distinct modules connected in tandem, namely insulin-like growth factor-binding protein (IGFBP)-like, von Willebrand factor (vWF) type C repeat, thrombospondin type 1 (TSP-1) repeat, and carboxyl-terminal (CT) modules. The relationships between each of these modules of CTGF and RA remain unknown. Here, we analyzed how inhibition of each CTGF module affects the pathophysiology of RA...
2016: Autoimmunity
https://www.readbyqxmd.com/read/26453509/cucurbitacin-e-inhibits-tnf-%C3%AE-induced-inflammatory-cytokine-production-in-human-synoviocyte-mh7a-cells-via-suppression-of-pi3k-akt-nf-%C3%AE%C2%BAb-pathways
#13
Qingyun Jia, Wenxiang Cheng, Ye Yue, Yipping Hu, Jian Zhang, Xiaohua Pan, Zhanwang Xu, Peng Zhang
Increasing studies indicated that Cucurbitacin E (CuE), a compound isolated from Cucurbitaceae, has been shown anti-inflammatory effect. However, the effect of CuE on rheumatoid arthritis (RA) inflammatory response and its potential molecular mechanism are still unknown. In this study, we demonstrated that CuE significantly suppressed TNF-α-induced inflammatory cytokines production interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8) mRNA and protein expression in human synoviocyte MH7A cells...
December 2015: International Immunopharmacology
https://www.readbyqxmd.com/read/26370562/o-linked-n-acetylglucosamine-glycosylation-of-p65-aggravated-the-inflammation-in-both-fibroblast-like-synoviocytes-stimulated-by-tumor-necrosis-factor-%C3%AE-and-mice-with-collagen-induced-arthritis
#14
Han Byeol Kim, Sang Won Lee, Chin Hee Mun, Ji Young Yoon, Jaeyoung Pai, Injae Shin, Yong Beom Park, Soo Kon Lee, Jin Won Cho
INTRODUCTION: We investigated the inflammatory potential of O-linked N-acetylglucosamine glycosylation (O-GlcNAcylation) of p65 in rheumatoid arthritis (RA). METHODS: Fibroblast-like synoviocytes (FLS) and MH7A cells were treated with synthetic ThiaMet-G (200 μM), an O-GlcNAcase (OGA) inhibitor, followed by tumor necrosis factor (TNF)-α (10 μg/mL). Proliferation of synovial cells was measured by MTT assay, and the levels of mRNAs encoding pro-inflammatory molecules were quantitated by RT-PCR...
2015: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/26267111/inhibitory-effects-of-h-ras-raf-1-binding-affibody-molecules-on-synovial-cell-function
#15
Seiji Shibasaki, Miki Karasaki, Torbjörn Gräslund, Per-Åke Nygren, Hajime Sano, Tsuyoshi Iwasaki
Affibody molecules specific for H-Ras and Raf-1 were evaluated for their ability to inhibit synovial cell function. Affibody molecules targeting H-Ras (Zras122, Zras220, and Zras521) or Raf-1 (Zraf322) were introduced into the MH7A synovial cell line using two delivery methods: transfection with plasmids encoding the affibody molecules or direct introduction of affibody protein using a cell-penetrating peptide reagent. Interleukin-6 (IL-6) and prostaglandin E2 (PGE2) production by MH7A cells were analyzed by enzyme-linked immunosorbent assay after stimulation with tumor necrosis factor-alpha (TNF-α)...
December 2014: AMB Express
https://www.readbyqxmd.com/read/25862966/selective-modulation-of-mapks-contribute-to-the-anti-proliferative-and-anti-inflammatory-activities-of-1-7-dihydroxy-3-4-dimethoxyxanthone-in-rheumatoid-arthritis-derived-fibroblast-like-synoviocyte-mh7a-cells
#16
Jian Zuo, Yan Xia, Xiang Li, Zhen Ou-Yang, Jian-wei Chen
ETHNOPHARMACOLOGICAL RELEVANCE: 1,7-Dihydroxy-3,4-dimethoxyxanthone (XAN) is an antirheumatic agent isolated from traditional Chinese medicine Securidaca inappendiculata Hassk. This study was designed to investigate its anti-proliferative and anti-inflammatory activities on rheumatoid arthritis derived fibroblast-like synoviocyte cell line MH7A, and explore the underlying mechanism of action. METHODS: The anti-proliferative activity of XAN on MH7A cells was assessed by an MTT method...
June 20, 2015: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/25332857/differential-regulation-of-c-met-signaling-pathways-for-synovial-cell-function
#17
Seiji Shibasaki, Sachi Tsunemi, Sachie Kitano, Masahiro Sekiguchi, Hajime Sano, Tsuyoshi Iwasaki
We previously demonstrated that blocking the hepatocyte growth factor (HGF) receptor, c-Met, using a HGF antagonist, NK4, inhibited arthritis in a rheumatoid arthritis (RA) model mice. In the present study, we investigated the role of c-Met signaling in synovial cell function. We demonstrated that synovial tissues from RA patients and MH7A cells, a human RA synovial cell line, expressed HGF and c-Met. HGF and c-Met expression in RA synovium was increased compared to osteoarthritis synovium suggesting increased c-Met signaling in RA synovial cells...
2014: SpringerPlus
https://www.readbyqxmd.com/read/25071309/reduction-of-il-20-expression-in-rheumatoid-arthritis-by-linear-polarized-infrared-light-irradiation
#18
Asayo Imaoka, Lin Zhang, Noboru Kuboyama, Yoshimitsu Abiko
BACKGROUND: Low-level laser is being evaluated for treating rheumatoid arthritis (RA). Recently, the linear polarized infrared light (Super Lizer, SL) irradiation may also be useful for RA treatment. However, the molecular mechanism underlying the effectiveness of SL on RA is unclear. It has been IL-20 may involved in RA disease progression. AIM: To understand how SL action, we constructed the experimental model in vitro using human rheumatoid fibroblast-like synoviocyte (MH7A) and collagen induced (CIA) RA rat in vivo...
July 1, 2014: Laser Therapy
https://www.readbyqxmd.com/read/24814038/hempseed-oil-induces-reactive-oxygen-species-and-c-ebp-homologous-protein-mediated-apoptosis-in-mh7a-human-rheumatoid-arthritis-fibroblast-like-synovial-cells
#19
Mini Jeong, Jaewook Cho, Jong-Il Shin, Yong-Joon Jeon, Jin-Hyun Kim, Sung-Joon Lee, Eun-Soo Kim, Kyungho Lee
ETHNOPHARMACOLOGICAL RELEVANCE: The medicinal efficacy of hempseed (Cannabis sativa L.), which is rich in polyunsaturated fatty acids, in atopic dermatitis, inflammation, and rheumatoid arthritis (RA) has been suggested for centuries. Hempseed has been used as a treatment for these diseases in Korean and Chinese folk medicine. The aim of the study is to investigate the effects of hempseed oil (HO) on MH7A human RA fibroblast-like synovial cells. MATERIALS AND METHODS: MH7A cells were used to study the anti-rheumatoid effects of hempseed (Cannabis sativa L...
July 3, 2014: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/24688409/effects-of-thapsigargin-on-the-proliferation-and-survival-of-human-rheumatoid-arthritis-synovial-cells
#20
Hui Wang, Xiu-zhi Jia, Chun-jie Sui, Yan-ping Zhao, Yi-fang Mei, Yi-ning Zheng, Zhi-yi Zhang
A series of experiments have been carried out to investigate the effects of different concentrations of thapsigargin (0, 0.001, 0.1, and 1 μM) on the proliferation and survival of human rheumatoid arthritis synovial cells (MH7A). The results showed that thapsigargin can block the cell proliferation in human rheumatoid arthritis synovial cells in a time- and dose-dependent manner. Results of Hoechst staining suggested that thapsigargin may induce cell apoptosis in MH7A cells in a time- and dose-dependent manner, and the percentages of cell death reached 44...
2014: TheScientificWorldJournal
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