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Jong-Joo Lee, Mikyoung Kim, Hyoung-Pyo Kim
Special AT-rich sequence binding protein 1 (SATB1) is a nuclear matrix-associated DNA-binding protein that functions as a chromatin organizer. SATB1 is highly expressed in aggressive breast cancer cells and promotes growth and metastasis by reprograming gene expression. Through genome-wide cross-examination of gene expression and histone methylation, we identified SATB1 target genes for which expression is associated with altered epigenetic marks. Among the identified genes, long noncoding RNA urothelial carcinoma-associated 1 (UCA1) was upregulated by SATB1 depletion...
September 29, 2016: BMB Reports
Chuck C K Chao
Hepatitis B virus X protein (HBx) plays an important role in the development of hepatocellular carcinoma (HCC). In addition, hepatoma upregulated protein (HURP) is a cellular oncogene that is upregulated in a majority of HCC cases. We highlight here recent findings demonstrating a link between HBx, HURP and anti-apoptosis effects observed in cisplatin-treated HCC cells. We observed that Hep3B cells overexpressing HBx display increased HURP mRNA and protein levels, and show resistance to cisplatin-induced apoptosis...
September 8, 2016: World Journal of Hepatology
Christian H Gabriel, Fridolin Gross, Martin Karl, Heike Stephanowitz, Anna Floriane Hennig, Melanie Weber, Stefanie Gryzik, Ivo Bachmann, Katharina Hecklau, Jürgen Wienands, Johannes Schuchhardt, Hanspeter Herzel, Andreas Radbruch, Eberhard Krause, Ria Baumgrass
Transcription factors of the nuclear factor of activated T cell (NFAT)-family are essential for antigen-specific T cell activation and differentiation. Their cooperative DNA binding with other transcription factors, such as AP1-proteins (FOS, JUN, JUNB), FOXP3, IRFs and EGR1, dictate the gene regulatory action of NFATs. To identify as yet unknown interaction partners of NFAT, we purified biotin tagged NFATc1/αA, NFATc1/βC and NFATc2/C protein-complexes and analyzed their components by SILAC-based mass spectrometry...
September 16, 2016: Journal of Biological Chemistry
Michelle R Roberts, Lara E Sucheston-Campbell, Gary R Zirpoli, Michael Higgins, Jo L Freudenheim, Elisa V Bandera, Christine B Ambrosone, Song Yao
Single nucleotide polymorphisms (SNPs) in pathways influencing lymph node (LN) metastasis and estrogen receptor (ER) status in breast cancer may partially explain inter-patient variability in prognosis. We examined 154 SNPs in 12 metastasis-related genes for associations with breast cancer risk, stratified by LN and ER status, in European-American (EA) and African-American (AA) women. Two-thousand six hundred and seventy-one women enrolled in the Women's Circle of Health Study were genotyped. Pathway analyses were conducted using the adaptive rank truncated product (ARTP) method, with pARTP  ≤ 0...
September 6, 2016: Molecular Carcinogenesis
Simran Kaur, Yan Coulombe, Zubaidah M Ramdzan, Lam Leduy, Jean-Yves Masson, Alain Nepveu
Base excision repair is initiated by DNA glycosylases that recognize specific altered bases. DNA glycosylases for oxidized bases carry both a glycosylase activity that removes the faulty base and an apyrimidinic/apurinic (AP)-lyase activity that introduces a single-strand DNA incision. In particular, the CUT domains within the CUX1 and CUX2 proteins were recently shown to interact with the 8-oxoguanine DNA glycosylase and stimulate its enzymatic activities. SATB1, which contains two CUT domains, was originally characterized as a T-cell specific genome organizer whose aberrant overexpression in breast cancer can promote tumor progression...
September 2, 2016: Journal of Biological Chemistry
Isabelle Chang, Marta Parrilla
Homeodomain proteins are encoded by homeobox genes and regulate development and differentiation in many neuronal systems. The mouse vomeronasal organ (VNO) generates in situ mature chemosensory neurons from stem cells. The roles of homeodomain proteins in neuronal differentiation in the VNO are poorly understood. Here we have characterized the expression patterns of 28 homeobox genes in the VNO of C57BL/6 mice at postnatal stages using multicolor fluorescent in situ hybridization. We identified 11 homeobox genes (Dlx3, Dlx4, Emx2, Lhx2, Meis1, Pbx3, Pknox2, Pou6f1, Tshz2, Zhx1, Zhx3) that were expressed exclusively in neurons; 4 homeobox genes (Pax6, Six1, Tgif1, Zfhx3) that were expressed in all non-neuronal cell populations, with Pax6, Six1 and Tgif1 also expressed in some neuronal progenitors and precursors; 12 homeobox genes (Adnp, Cux1, Dlx5, Dlx6, Meis2, Pbx2, Pknox1, Pou2f1, Satb1, Tshz1, Tshz3, Zhx2) with expression in both neuronal and non-neuronal cell populations; and one homeobox gene (Hopx) that was exclusively expressed in the non-sensory epithelium...
July 2016: Gene Expression Patterns: GEP
Hironobu Baba, Toshiaki Ishikawa, Kaoru Mogushi, Megumi Ishiguro, Hiroyuki Uetake, Hiroshi Tanaka, Kenichi Sugihara
BACKGROUND/AIM: The integration of gene expression analysis and genomic profiling represents an efficient approach to the discovery of cancer-related genes. Lymph node metastasis (LNM) is a significant prognostic factor in colorectal cancer (CRC). Detection and analysis of factors related to LNM will help develop new diagnostic methods or therapies. In this study, we aimed to identify genes that are significantly related to LNM in CRC through integrated copy number analysis (CNA) and gene expression analysis...
August 2016: Anticancer Research
Kazuhiko Yamasaki, Tomoko Yamasaki
Transcription factor SATB1 (special AT-rich sequence binding protein 1) contains multiple DNA-binding domains (DBDs), i.e. two CUT-domain repeats (CUTr1 and CUTr2 from the N-terminus) and a homeodomain, and binds to the matrix attachment region (MAR) of DNA. Although CUTr1 and the homeodomain, but not CUTr2, are known to contribute to DNA binding, different research groups have not reached a consensus on which DBD is responsible for recognition of the target sequence in MAR, 5'-TAATA-3'. Here, we used isothermal titration calorimetry to demonstrate that CUTr1 has binding specificity to this motif, whereas the homeodomain shows affinity for a variety of DNAs without specificity...
October 1, 2016: Biochemical Journal
Kamalvishnu P Gottimukkala, Rahul Jangid, Indumathi Patta, Dil Afroz Sultana, Archna Sharma, Jyoti Misra-Sen, Sanjeev Galande
T lymphocyte development and differentiation is a multi-step process that begins in the thymus and completed in the periphery. Sequential development of thymocytes is dependent on T cell receptor (TCR) signaling and an array of transcription factors. In this study we show that special AT-rich binding protein 1 (SATB1), a T lineage-enriched chromatin organizer and regulator, is induced in response to TCR signaling during early thymocyte development. SATB1 expression profile coincides with T lineage commitment and upregulation of SATB1 correlates with positive selection of thymocytes...
September 2016: Molecular Immunology
M Jargosch, S Kröger, E Gralinska, U Klotz, Z Fang, W Chen, U Leser, J Selbig, D Groth, R Baumgrass
Data integration has become a useful strategy for uncovering new insights into complex biological networks. We studied whether this approach can help to delineate the signal transducer and activator of transcription 6 (STAT6)-mediated transcriptional network driving T helper (Th) 2 cell fate decisions. To this end, we performed an integrative analysis of publicly available RNA-seq data of Stat6-knockout mouse studies together with STAT6 ChIP-seq data and our own gene expression time series data during Th2 cell differentiation...
2016: Genetics and Molecular Research: GMR
C-L Yuan, L Li, X Zhou, H Liz, L Han
OBJECTIVE: To detect expression of HER2 and SATB1 in paraffin tissues of gastric cancer, and to investigate their relationship with clinic pathological factors and prognosis, and further analyze the correlation between the expression of SATB1 and HER2. PATIENTS AND METHODS: RT-PCR was used to determine the expression of SATB1 gene and immunohistochemistry (IHC) was used to detect the expression of HER2 protein in gastric cancer tissues. The relationships of both were evaluated with clinic pathological parameters and prognosis, and further analyzed to build a correlation between the expression of SATB1 and HER2...
June 2016: European Review for Medical and Pharmacological Sciences
Qiang Wu, Gang Wu, Jing-Xiang Li
OBJECTIVE: To study the effect of hypoxia on the expression of placental trophoblast cells SATB1 and β-catenin and its correlation with the pathogenesis of preeclampsia. METHODS: Trophoblastic cell lines HRT8/SVneo were cultured, SATB1 and β-catenin expression and cell biological behavior were determined after hypoxia reoxygenation treatment; cell biological behavior and the expression of related genes were determined after the transfection of SATB1 and β-catenin siRNA; preeclampsia placenta and normal placenta tissues were collected and the expression of SATB1 and β-catenin were determined...
June 2016: Asian Pacific Journal of Tropical Medicine
Liguo Geng, Meng Zhu, Yuzhuo Wang, Yang Cheng, Jia Liu, Wei Shen, Zhihua Li, Jiahui Zhang, Cheng Wang, Guangfu Jin, Hongxia Ma, Hongbing Shen, Zhibin Hu, Juncheng Dai
Chromatin remodeling complexes utilize the energy of ATP hydrolysis to remodel nucleosomes and have essential roles in transcriptional modulation. Increasing evidences indicate that these complexes directly interact with numerous proteins and regulate the formation of cancer. However, few studies reported the association of polymorphisms in chromatin remodeling genes and lung cancer. We hypothesized that variants in critical genes of chromatin remodeling pathway might contribute to the susceptibility of lung cancer...
August 10, 2016: Gene
Zhongya Pan, Wei Jing, Keli He, Li Zhang, Xinghua Long
BACKGROUND/AIMS: Several researches have evaluated the significance of SATB1 (Special AT-rich sequence binding protein 1) expression in breast cancers (BCs), but the results have been disputed, especially in the aspects of clinicopathological features and prognosis. Therefore, our study aimed to use a meta-analysis to summarize the clinical and prognostic relevance of SATB1 gene expression in BCs. METHODS: A literature search of PubMed, EMBASE, Cochrane library, Chinese Wanfang and CNKI was performed to identify eligible studies...
2016: Cellular Physiology and Biochemistry
Xingcong Ma, Wanjun Yan, Zhijun Dai, Xiaoyan Gao, Yinan Ma, Quntao Xu, Jiantao Jiang, Shuqun Zhang
BACKGROUND: The flavonoid baicalein, a historically used Chinese herbal medicine, shows a wide range of biological and pharmaceutical effects, among which its potent antitumor activity has raised great interest in recent years. However, the molecular mechanism involved in the antimetastatic effect of baicalein remains poorly understood. This study aimed to verify the inhibitory effects of baicalein on metastasis of MDA-MB-231 human breast cancer cells both in vitro and in vivo, as well as to investigate the related mechanisms...
2016: Drug Design, Development and Therapy
Anna E Kowalczyk, Bartlomiej E Krazinski, Janusz Godlewski, Jedrzej Grzegrzolka, Jolanta Kiewisz, Przemyslaw Kwiatkowski, Agnieszka Sliwinska-Jewsiewicka, Piotr Dziegiel, Zbigniew Kmiec
BACKGROUND: Altered expression of special AT-rich sequence binding protein 1 (SATB1) was reported in several types of human cancers. This study aimed to determine the expression levels of SATB1, as well as miR-21-5p -the post-transcriptional repressor of SATB1 expression- in clear cell renal cell carcinoma (ccRCC) and to investigate their association with the progression of ccRCC. MATERIALS AND METHODS: Immunohistochemistry and quantitative polymerase chain reaction were used to assess the expression of SATB1 protein and mRNA as well as miR-21-5p in tumor and matched normal kidney tissues collected from 56 ccRCC patients...
May 2016: Cancer Genomics & Proteomics
Warren Winick-Ng, Fabiana A Caetano, Jennifer Winick-Ng, Trevor M Morey, Bryan Heit, R Jane Rylett
The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1-42 (Aβ1-42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1-42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1-42-exposure increases 82-kDa ChAT association with gene promoters and introns...
2016: Scientific Reports
Li-jun Mao, Chun-hua Yang, Li Fan, Peng Gao, Dong-rong Yang, Bo-xin Xue, Jun-nian Zheng, Yu-Xi Shan
Special AT-rich sequence binding protein 1 (SATB1) plays important role in the regulation of chromatin structure and gene expression. Recent studies have indicated oncogenic role of SATB1. However, the function of SATB1 in prostate cancer progression and metastasis remains unclear. In this study SATB1 expression vector or siRNA was employed to modulate the expression level of SATB1 in prostate cancer cells and xenograft tumor in nude mouse model. Immunohistochemical analysis was performed on clinical prostate cancer samples...
April 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Motonari Kondo, Yuriko Tanaka, Taku Kuwabara, Taku Naito, Terumi Kohwi-Shigematsu, Akiko Watanabe
No abstract text is available yet for this article.
April 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Marshall E Kadin, Anand Deva, Haiying Xu, John Morgan, Pranay Khare, Roderick A F MacLeod, Bruce W Van Natta, William P Adams, Garry S Brody, Alan L Epstein
Almost 200 women worldwide have been diagnosed with breast implant-associated anaplastic large cell lymphoma (BIA-ALCL). The unique location and specific lymphoma type strongly suggest an etio-pathologic link between breast implants and BIA-ALCL. It is postulated that chronic inflammation via bacterial infection may be an etiological factor. BIA-ALCL resembles primary cutaneous ALCL (pcALCL) in morphology, activated T-cell phenotype, and indolent clinical course. Gene expression array analysis, flow cytometry, and immunohistochemistry were used to study pcALCL and BIA-ALCL cell lines...
July 2016: Aesthetic Surgery Journal
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