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Flow cytometry and exercise

Angela D'Ascola, Giuseppe Bruschetta, Gabriella Zanghì, Salvatore Campo, Pietro Medica, Stefania Campana, Guido Ferlazzo, Bernhard F Gibbs, Alida Maria Ferlazzo
Serotonin (5-HT) is a neurohormone transported from plasma into platelets and leukocytes by a specific transporter (SERT). While it is known that the brain 5-HT system is modulated by physical exercise, the peripheral serotoninergic response to exercise is not yet fully elucidated. In particular, this study aimed to evaluate changes in plasma 5-HT levels and equine leukocyte SERT expression in response to treadmill exercise in untrained horses. Analyses were carried out pre- and post-treadmill exercise. 5-HT plasma levels were analysed by HPLC...
February 27, 2018: Research in Veterinary Science
Artur Juszkiewicz, Piotr Basta, Elżbieta Petriczko, Bogusław Machaliński, Jerzy Trzeciak, Karolina Łuczkowska, Anna Skarpańska-Stejnborn
Background: The aim of this study was to analyze the response of selected components of the immune system in rowers to maximal physical exercise, and to verify if this response can be modulated by supplementation with spirulina (cyanobacterium Spirulina platensis ) . Method: The double-blind study included 19 members of the Polish Rowing Team. The subjects were randomly assigned to the supplemented group ( n  = 10), receiving 1500 mg of spirulina extract for 6 weeks, or to the placebo group ( n  = 9)...
2018: Journal of the International Society of Sports Nutrition
Luciele G Minuzzi, Luís Rama, Matheus Uba Chupel, Fátima Rosado, João Valente Dos Santos, Richard Simpson, António Martinho, Artur Paiva, Ana M Teixeira
BACKGROUND/PURPOSE: Ageing has profound impact on the immune system, mainly on T-cells. However, it has been suggested that chronic exercise may delay immunosenescence. Master athletes represent an interesting sub-demographic group to test this theory since they maintain a high training frequency and load throughout life. The purpose of this study was to evaluate the effects of lifelong training on the senescence and mobilization of T lymphocytes in response to acute exercise. MATERIAL AND METHODS: Nineteen athletes who regularly participated in training and competitions for more than 20 years throughout their lives and a control group of 10 healthy individuals participated in this study...
2018: Exercise Immunology Review
Zhisong Chen, Wenwen Yan, Yu Mao, Yi Ni, Lin Zhou, Haoming Song, Wenjun Xu, Leming Wang, Yuqin Shen
Immune activation and inflammation participate in the progression of chronic heart failure (CHF). Th17 cells and CD4+CD25+ regulatory T (Treg) cells both come from naive Th cells and share reciprocal development pathways but exhibit opposite effects. We hypothesized that the Th17/Treg balance was impaired in patients with CHF, and exercise can improve it. Rats with ischemic cardiomyopathy were prepared by ligaturing the left anterior descending branch of the left coronary artery. Rats in training group were trained with treadmill; Th17 cells increased significantly while Treg cells significantly decreased in s by flow cytometry, and the peripheral blood level of IL-6, IL-17, and TNF-α was obviously elevated by ELISA assay...
February 16, 2018: Journal of Cardiovascular Translational Research
Andreas Asimakos, Dimitrios Toumpanakis, Maria-Helena Karatza, Spyridoula Vasileiou, Paraskevi Katsaounou, Zafeiria Mastora, Theodoros Vassilakopoulos
Background/hypothesis: Whole body exercise (WBE) changes lymphocyte subset percentages in peripheral blood. Resistive breathing, a hallmark of diseases of airway obstruction, is a form of exercise for the inspiratory muscles. Strenuous muscle contractions induce oxidative stress that may mediate immune alterations following exercise. We hypothesized that inspiratory resistive breathing (IRB) alters peripheral blood lymphocyte subsets and that oxidative stress mediates lymphocyte subpopulation alterations following both WBE and IRB...
2018: International Journal of Chronic Obstructive Pulmonary Disease
Elie Nader, Nicolas Guillot, Louis Lavorel, Ivan Hancco, Romain Fort, Emeric Stauffer, Céline Renoux, Philippe Joly, Michèle Germain, Philippe Connes
We compared the effects of cycling and running exercise on hemorheological and hematological properties, as well as eryptosis markers. Seven endurance-trained subjects randomly performed a progressive and maximal exercise test on a cycle ergometer and a treadmill. Blood was sampled at rest and at the end of the exercise to analyze hematological and blood rheological parameters including hematocrit (Hct), red blood cell (RBC) deformability, aggregation and blood viscosity. Hemoglobin saturation (SpO2), blood lactate and glucose levels were also monitored...
January 21, 2018: Scandinavian Journal of Medicine & Science in Sports
José P Morgado, Cristina P Monteiro, Catarina N Matias, Joana F Reis, Júlia Teles, Maria José Laires, Francisco Alves
PURPOSE: Long-term training influence on athletes' immune cell response to acute exercise has been poorly studied, despite the complexity of both chronic and acute adaptations induced by training. The purpose of the study is to study the influence of a 4-month swimming training cycle on the immune cell response to a high-intensity training session, during 24 h of recovery, considering sex, maturity, and age group. METHODS: Forty-three swimmers (16 females, 14.4 ± 1...
January 8, 2018: European Journal of Applied Physiology
Yihe Zhao, Nana Li, Zhu Li, Da Zhang, Liming Chen, Zhi Yao, Wenyan Niu
OBJECTIVE: Obese adipose tissue has been characterized with chronic inflammation associated with elevated secretion of inflammatory cytokines and declined secretion of anti-inflammatory cytokines which can impair endothelial function in an endocrine manner. Adipose tissue hypoxia plays a role in the changes of cytokines. Physical exercise / muscle contraction may help preventing cardiovascular disease through improving insulin resistance and endothelium function. However the mechanism is unclear...
December 28, 2017: Metabolism: Clinical and Experimental
Mark Daniel Ross, Eva M Malone, Richard Simpson, Islay Cranston, Lesley Ingram, Graham P Wright, George Chambers, Geraint D Florida-James
Ageing is associated with a dysfunctional endothelial phenotype, as well as reduced angiogenic capabilities. Exercise exerts beneficial effects on the cardiovascular system, possibly by increasing/maintaining the number and/or function of circulating angiogenic cells (CACs) that are known to decline with age. However, the relationship between cardiorespiratory fitness (CRF) and age related changes in frequency of CACs, as well as the exercise-induced responsiveness of CACs in older individuals has not yet been determined...
November 22, 2017: American Journal of Physiology. Heart and Circulatory Physiology
Aaron L Slusher, Tiffany M Zúñiga, Edmund O Acevedo
PURPOSE: Monocytes express the CD14 receptor that facilitates lipopolysaccharide (LPS) ligation to toll-like receptor 4 (TLR4) to elicit production of interleukin (IL)-6, IL-10, and tumor necrosis factor alpha (TNF-α). However, pro-inflammatory conditions, such as strenuous exercise, increase the percentage of monocytes expressing CD16, a receptor that enhances LPS stimulated TNF-α production. Therefore, we examined whether maximal treadmill exercise would alter the inflammatory phenotype of classical (CD14/CD16) and pro-inflammatory monocytes (intermediate [CD14/CD16] and non-classical [CD14/CD16]), evidenced by changes in TLR4, CD14, and CD16 receptor expression, and their inflammatory response to ex vivo LPS stimulation...
November 6, 2017: Medicine and Science in Sports and Exercise
Karsten Krüger, Michael Seimetz, Robert Ringseis, Jochen Wilhelm, Alexandra Pichl, Aline Couturier, Klaus Eder, Norbert Weissmann, Frank C Mooren
Long term cigarette smoking induces inflammatory processes in the pulmonary system which are suggested to "spill over" into systemic inflammation. Regular exercise has been shown to have anti-inflammatory properties. The aim of the study was to investigate the effects of therapeutic exercise on inflammation and muscle wasting in smoke-exposed mice. C57BL/6J-mice (n = 30) were separated into three groups to receive either: (1) controls with no specific treatment (Con group), (2) 8 months exposure to cigarette smoke (smoke-exposed (SE) group), (3) 8 months of cigarette smoke combined with exercise training during the last 2 months (SEex group)...
November 1, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Nadia H Agha, Forrest L Baker, Hawley E Kunz, Rachel Graff, Rod Azadan, Chad Dolan, Mitzi S Laughlin, Chitra Hosing, Melissa M Markofski, Richard A Bond, Catherine M Bollard, Richard J Simpson
Acute dynamic exercise mobilizes CD34+ hematopoietic stem cells (HSCs) to the bloodstream, potentially serving as an economical adjuvant to boost the collection of HSCs from stem cell transplant donors. The mechanisms responsible for HSC mobilization with exercise are unknown but are likely due to hemodynamic perturbations, endogenous granulocyte-colony stimulating factor (G-CSF), and/or β2-adrenergic receptor (β2-AR) signaling. We characterized the temporal response of HSC mobilization and plasma G-CSF following exercise, and determined the impact of in vivo β-AR blockade on the exercise-induced mobilization of HSCs...
October 7, 2017: Brain, Behavior, and Immunity
Dongwei Wang, Yi Wang, Juan Ma, Wenping Wang, Bingbing Sun, Tengfei Zheng, Ming Wei, Yangli Sun
BACKGROUND: Exercise can reduce the coronary artery disease (CAD) incidence. MiRNA-20a has been reported to distinctly expressed after sustain physical activity. However, its expression and regulation pattern in CAD model with or without exercise has not been reported. OBJECTIVE: In present study, we aim to investigate regulatory mechanism of miR-20a in exercise-associated reduced-CAD incidence and miR-20a-dependent signaling pathways. METHODS: Eight weeks old male ApoE/LDLR double knock out mice were recruited for this study...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Erik D Hanson, Eli Danson, Catriona V Nguyen-Robertson, Jackson J Fyfe, Nigel K Stepto, David B Bartlett, Samy Sakkal
PURPOSE: Mucosal associated invariant T (MAIT) cells have properties of the innate and acquired immune systems. While the response to vigorous exercise has been established for most leukocytes, MAIT cells have not been investigated. Therefore, the purpose was to determine if MAIT cell lymphocytosis occurs with acute maximal aerobic exercise and if this response is influenced by exercise duration, cardiovascular fitness, or body composition. METHODS: Twenty healthy young males with moderate fitness levels performed an extended graded exercise test until volitional fatigue...
November 2017: European Journal of Applied Physiology
Junhao Huang, Shen Wang, Fengpeng Xu, Dan Wang, Honggang Yin, Qinhao Lai, Jingwen Liao, Xiaohui Hou, Min Hu
OBJECTIVE: Circulating endothelial progenitor cells (EPCs) correlate negatively with obesity. Previous studies have shown that exercise significantly restores circulating EPC levels in obese people; however, the underlying mechanisms have not been elucidated. Recently, irisin has been reported to have a critical role in the regulation of EPCs. This exercise-induced myokine has been demonstrated to play a therapeutic role in obesity. In this study, we hypothesized that the increase in circulating irisin may form a link with increasing EPC levels in obese people after exercise...
2017: PeerJ
Junichiro Miake, Tomomi Notsu, Katsumi Higaki, Kyoko Hidaka, Takayuki Morisaki, Kazuhiro Yamamoto, Ichiro Hisatome
Cardiac progenitor cells have a limited proliferative capacity. The CREB-binding protein/p300-interacting transactivator, with the Glu/Asp-rich carboxy-terminal domain (Cited) gene family, regulates gene transcription. Increased expression of the Cited4 gene in an adult mouse is associated with exercise-induced cardiomyocyte hypertrophy and proliferation. However, the expression patterns and functional roles of the Cited4 gene during cardiogenesis are largely unknown. Therefore, in the present study, we investigated the expression patterns and functional roles of the Cited4 gene during in vitro cardiogenesis...
2017: PloS One
Grace M Niemiro, Thomas Edwards, J P Barfield, Joseph W Beals, Elizabeth M Broad, Robert W Motl, Nicholas A Burd, Lara A Pilutti, Michael De Lisio
INTRODUCTION: Circulating progenitor cells (CPCs) are a heterogeneous population of stem/progenitor cells in peripheral blood that participate in tissue repair. CPC mobilization has been well characterized in able-bodied persons, but has not been previously investigated in wheelchair racing athletes. The purpose of this study was to characterize CPC and CPC sub-population mobilization in elite wheelchair racing athletes in response to acute, upper-extremity aerobic exercise to determine if CPC responses are similar to ambulatory populations...
August 11, 2017: Medicine and Science in Sports and Exercise
Kim D Lu, Dan Cooper, Fadia Haddad, Frank Zaldivar, Monica Kraft, Shlomit Radom-Aizik
BackgroundPoor aerobic fitness is associated with worsening of asthma symptoms, and fitness training may improve asthma control. The mechanism linking fitness with asthma is not known. We hypothesized that repeated bouts of exercise would lead to a downregulation of glucocorticoid receptor (GR) expression on circulating leukocytes, reflecting a reduced responsiveness to stress.MethodsIn a prospective exercise training intervention of healthy and asthmatic adolescents, GR expression in leukocytes was measured using flow cytometry in response to an acute exercise challenge before and after the exercise training intervention...
August 2017: Pediatric Research
Camila Iansen Irion, Bruno Diaz Paredes, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, Luis Felipe Paula, Alysson Roncally Carvalho, Antonio Carlos Campos de Carvalho, Adriana Bastos Carvalho, Regina Coeli Dos Santos Goldenberg
BACKGROUND: Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare...
August 2017: Memórias do Instituto Oswaldo Cruz
Rafael Evangelista Pedro, Nadia Candido, Débora Alves Guariglia, Bruno Pereira Melo, Dennis Armando Bertolini, Sidney Barnabé Peres, Solange Marta Franzói de Moraes
PURPOSE: Verify the effects of concurrent training on cytokines in people living with HIV under antiretroviral therapy (ART) treatment. METHODS: This was a blinded, parallel-group, clinical trial, where 49 participants, divided in two groups, either control group or concurrent training group, took part in the intervention. The control group performed recreational activities and concurrent training group participated of 16-week, 3 times per week of heart rate guided-aerobic plus resistance training for major muscular groups...
July 3, 2017: Cytokine
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