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Flow cytometry and exercise

Ana L Kolicheski, Gary S Johnson, Tendai Mhlanga-Mutangadura, Jeremy F Taylor, Robert D Schnabel, Taroh Kinoshita, Yoshiko Murakami, Dennis P O'Brien
Hereditary paroxysmal dyskinesias (PxD) are a heterogeneous group of movement disorders classified by frequency, duration, and triggers of the episodes. A young-adult onset canine PxD has segregated as an autosomal recessive trait in Soft-Coated Wheaten Terriers. The medical records and videos of episodes from 25 affected dogs were reviewed. The episodes of hyperkinesia and dystonia lasted from several minutes to several hours and could occur as often as >10/day. They were not associated with strenuous exercise or fasting but were sometimes triggered by excitement...
November 28, 2016: Neurogenetics
Jeff M Baker, Joshua P Nederveen, Gianni Parise
Hematopoietic stem and progenitor cells are necessary to maintain, repair, and reconstitute the hematopoietic blood cell system. Mobilization of these cells from bone marrow to blood can be greatly increased under certain conditions, one such being exercise. The purpose of this study was to identify the importance of exercise intensity in hematopoietic mobilization, to better understand the mobilization kinetics post exercise, and to determine if exercise is capable of mobilizing several specific populations of hematopoietic cells that have clinical relevance in a transplant setting...
November 23, 2016: Journal of Applied Physiology
Jéssica García-Lucio, Olga Tura-Ceide, Roberto Del Pozo, Isabel Blanco, Sandra Pizarro, Elisabet Ferrer, Marta Díez, Núria Coll-Bonfill, Lucilla Piccari, Víctor I Peinado, Joan Albert Barberà
BACKGROUND: Endothelial dysfunction is key in the development of pulmonary hypertension (PH) and is associated with reduced number of circulating progenitor cells. Studies to date evaluating levels of circulating progenitor cells in PH have provided conflicting results. Current treatment of pulmonary arterial hypertension (PAH) and medical treatment of chronic thromboembolic pulmonary hypertension (CTEPH) targets endothelium dependent signalling pathways. The effect of PAH-targeted therapy on circulating progenitor cells has not been clearly established...
November 9, 2016: International Journal of Cardiology
Dorota Halicka, Hong Zhao, Jiangwei Li, Jorge Garcia, Monika Podhorecka, Zbigniew Darzynkiewicz
Cell synchronization is often achieved by transient inhibition of DNA replication. When cultured in the presence of such inhibitors as hydroxyurea, aphidicolin or excess of thymidine the cells that become arrested at the entrance to S-phase upon release from the block initiate progression through S then G2 and M. However, exposure to these inhibitors at concentrations commonly used to synchronize cells leads to activation of ATR and ATM protein kinases as well as phosphorylation of Ser139 of histone H2AX. This observation of DNA damage signaling implies that synchronization of cells by these inhibitors is inducing replication stress...
2017: Methods in Molecular Biology
Mary Pat Meaney, David C Nieman, Dru A Henson, Qi Jiang, Fu-Zhang Wang
The granulocyte and monocyte phagocytosis and oxidative burst (OB) activity assay can be used to study the innate immune system. This manuscript provides the necessary methodology to add this assay to an exercise immunology arsenal. The first step in this assay is to prepare two aliquots ("H" and "F") of whole blood (heparin). Then, dihydroethidium is added to the H aliquot, and both aliquots are incubated in a warm water bath followed by a cold water bath. Next, Staphylococcus aureus (S. aureus) is added to the H aliquot and fluorescein isothiocyanate-labeled S...
2016: Journal of Visualized Experiments: JoVE
Trevor Gillum, Matthew Kuennen, Zachary McKenna, Micaela Castillo, Alex Jordan-Patterson, Caitlin Bohnert
An increase in salivary leukocytes may contribute to the exercise-induced increase in salivary antimicrobial proteins (AMPs). However, exercise-induced changes in salivary leukocytes have not been studied. The purpose of the study was to describe salivary leukocyte changes with exercise. Participants (n = 11, 20.3 ± 0.8 years, 57.2 ± 7.6 ml kg(-1) min(-1) peak oxygen uptake ((VO) ̇2peak), 11.1 ± 3.9% body fat) ran for 45 min at 75% of VO2peak. Stimulated saliva (12 mL) was collected pre- and immediately post exercise...
August 22, 2016: Journal of Sports Sciences
Renyi Liu, Wei Fan, Karsten Krüger, Yu Xiao, Christian Pilat, Michael Seimetz, Robert Ringseis, Eveline Baumgart-Vogt, Klaus Eder, Norbert Weissmann, Frank-Christoph Mooren
PURPOSE: The study aimed to investigate the effects of chronic moderate exercise on regulation of intracellular calcium signaling as an important link to proliferation capacity in murine splenic T lymphocytes. METHODS: Male CD1 Swiss mice were randomly assigned either to a control group (CG) or an exercise group (EG). EG mice performed voluntary exercise for 3 months. Lymphocytes were isolated from murine spleens and intracellular calcium was determined using Fura-2(AM) and fluorescence spectrometry...
August 12, 2016: Medicine and Science in Sports and Exercise
Mark Ross, Peter Tormey, Lesley Ingram, Richard Simpson, Eva Malone, Geraint Florida-James
CD31(+) T-cells have been documented to possess vasculogenic properties, and have been termed 'angiogenic T-cells' (TANG ). No study to date has fully characterised the effect of acute exercise on TANG cells. Twelve male participants aged 24-45yrs undertook a running 10 km time trial, with peripheral blood samples taken pre-, immediately post- and 1-hour post-exercise for quantification of TANG cells and subsequent CXCR4 cell surface expression by flow cytometry. TANG cells demonstrated a 102% increase in number in peripheral circulation immediately post-exercise compared to pre-exercise levels, followed by a large egress (50%) from the circulation in total TANG cells 1-hour post-exercise...
July 18, 2016: Experimental Physiology
Daniel D Shill, Meagan P Marshburn, Hannah K Hempel, Kasey A Lansford, Nathan T Jenkins
PURPOSE: Circulating angiogenic cells (CACs) comprise multiple subpopulations of exercise-inducible peripheral blood mononuclear cells (PBMCs) that promote angiogenesis and maintain endothelial integrity. We examined the effect of acute maximal exercise on CD31, CD62E, CD14/CD31, CD34/VEGFR2, CD3/CD31, and CD3 PBMCs in young, healthy adults. METHODS: Blood samples were collected before and immediately after a graded treadmill exercise test for CAC analysis via flow cytometry...
July 6, 2016: Medicine and Science in Sports and Exercise
Elisa F D Canetti, J Keane, C P McLellan, A B Gray
PURPOSE: Compare capillary and venous blood in the analysis of concentration and function of leucocyte sub-populations. This study hypothesised that capillary samples may be used in a site-specific manner as an alternative source of blood samples for assays of leucocyte concentration and neutrophilic phagocytic function and reactive oxygen species (ROS) production, allowing acquisition of multiple samples to better monitor transient but significant post-exercise immune modulation. METHODS: Resting blood samples were simultaneously obtained from vein, finger and earlobe of healthy subjects (n = 10, age: 25...
August 2016: European Journal of Applied Physiology
Andrew H Lutz, Jacob B Blumenthal, Rian Q Landers-Ramos, Steven J Prior
Circulating endothelial progenitor cells (EPCs) contribute to vascular homeostasis and are fewer in those with type 2 diabetes mellitus (T2DM) compared with normal glucose tolerance (NGT), suggesting a link between EPCs and T2DM-associated vasculopathies. The purpose of this study was to assess EPC number and mobilization by acute submaximal exercise in older adults with NGT, impaired glucose tolerance (IGT) or T2DM. We tested the hypothesis that EPC mobilization is lower in IGT compared with NGT and further reduced in older adults with T2DM...
July 1, 2016: Journal of Applied Physiology
Karsten Krüger, Katharina Alack, Robert Ringseis, Lena Mink, Elisabeth Pfeifer, Matthias Schinle, Katharina Gindler, Lena Kimmelmann, Rüdiger Walscheid, Kerstin Muders, Torsten Frech, Klaus Eder, Frank-Christoph Mooren
INTRODUCTION: High-intensity interval training (HIT) exercise has gained much interest in both performance and recreational sports. This study aims to compare the effect of HIT versus continuous (CONT) exercise with regard to changes of circulating T cells and progenitor cells. METHODS: Subjects (n = 23) completed an HIT test and an isocaloric CONT test. Blood samples were collected before, immediately after, and 3 and 24 h postexercise for the assessment of low differentiated (CD3CD28CD57), highly differentiated T cells (CD3CD28CD57), regulatory T cells (Tregs) (CD4CD25CD127), hematopoietic progenitor cells (CD45CD34), and endothelial progenitor cells (CD45CD34KDR) by flow cytometry...
October 2016: Medicine and Science in Sports and Exercise
José P Morgado, Cristina P Monteiro, Júlia Teles, Joana F Reis, Catarina Matias, Maria T Seixas, Marta G Alvim, Mafalda Bourbon, Maria J Laires, Francisco Alves
Understanding the impact of training sessions on the immune response is crucial for the adequate periodization of training, to prevent both a negative influence on health and a performance impairment of the athlete. This study evaluated acute systemic immune cell changes in response to an actual swimming session, during a 24-h recovery period, controlling for sex, menstrual cycle phases, maturity, and age group. Competitive swimmers (30 females, 15 ± 1.3 years old; and 35 males, 16.5 ± 2.1 years old) performed a high-intensity training session...
May 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
Mariana A de Matos, Tamiris C Duarte, Vinícius de O Ottone, Pâmela F da M Sampaio, Karine B Costa, Marcos F Andrade de Oliveira, Pope L Moseley, Suzanne M Schneider, Cândido C Coimbra, Gustavo E A Brito-Melo, Flávio de C Magalhães, Fabiano T Amorim, Etel Rocha-Vieira
UNLABELLED: Obesity is a low-grade chronic inflammation condition, and macrophages, and possibly monocytes, are involved in the pathological outcomes of obesity. Physical exercise is a low-cost strategy to prevent and treat obesity, probably because of its anti-inflammatory action. We evaluated the percentage of CD16(-) and CD16(+) monocyte subsets in obese insulin-resistant individuals and the effect of an exercise bout on the percentage of these cells. Twenty-seven volunteers were divided into three experimental groups: lean insulin sensitive, obese insulin sensitive and obese insulin resistant...
June 2016: Cell Biochemistry and Function
Ryan P Steck, Spencer L Hill, Evita G Weagel, K Scott Weber, Richard A Robison, Kim L O'Neill
Caffeine is the most widely used neurostimulant in the world. There is considerable debate on its effect on immune cells as it has been shown to antagonize adenosine receptors (ARs), which mediate an anti-inflammatory switch in activated immune cells. A second target is phosphodiesterase, where it acts as an inhibitor. If the primary effect of caffeine on mononuclear phagocytes were to antagonize ARs we would expect cells exposed to caffeine to have a prolonged proinflammatory response. The aim of this study was to investigate the effects and mechanism of action of caffeine in mononuclear phagocytes...
December 2015: Pharmacology Research & Perspectives
Saman Ratnayake, Ali Ammar, Rodd Rezvani, Greti Petersen
We present a case of a 65-year-old Hispanic man with a history of disseminated cutaneous coccidioidomycosis who presented to the emergency room for progressively worsening abdominal pain associated with shortness of breath. The patient was found to have pleural effusion and moderate ascites on physical examination. Abdominal ultrasound and computed tomography scan were consistent with moderate ascites and portal hypertension but negative for both liver cirrhosis and for venous or arterial thrombosis. Cytology of ascitic fluid was suggestive of portal hypertension and was negative for infection...
October 2015: Journal of Investigative Medicine High Impact Case Reports
Jeff M Baker, Gianni Parise
PURPOSE: Erythropoietin is responsible for regulating the growth and development of red blood cells. Reports conflict on whether skeletal muscle is able to produce erythropoietin and release it into circulation and if exercise affects this. We set out to determine how erythropoietin is regulated in skeletal muscle and to determine whether skeletal muscle-derived erythropoietin can stimulate erythropoiesis. METHODS: Using an in vitro approach, we exposed proliferating and differentiated skeletal muscle cells to various forms of exercise-induced physiological stimuli and measured erythropoietin gene expression...
July 2016: Medicine and Science in Sports and Exercise
Huifang Jiang, Zhonglin Wu, L I Ren, Diehong Tao, Hongyan Tong
Atypical chronic myeloid leukemia (aCML) is a hematopoietic stem/progenitor cell disorder, predominantly involving neutrophils. At present, a limited number of studies regarding the treatment of aCML have been published, and the therapies that are currently available exhibit unsatisfactory outcomes. In the present study, the cases of two aCML patients treated with decitabine (DCA) therapy who achieved remission are presented. A 48-year-old male, who presented with fatigue and a cough that had lasted two months, and a 69-year-old male who presented with dizziness, fatigue and shortness of breath with exercise, were diagnosed with aCML following bone marrow examination, flow cytometry and chromosome banding analysis...
January 2016: Oncology Letters
Wu Zhou, Guo-hui Liu, Shu-hua Yang, Bo-bin Mi, Shu-nan Ye
In order to investigate the promoting effect of low-intensity treadmill exercise on rat dorsal wound healing and the mechanism, 20 Sprague-Dawley rats were randomly divided into two groups: exercise group (Ex) and non-exercise group (non-ex). The rats in Ex group were given treadmill exercise for one month, and those in non-ex group raised on the same conditions without treadmill exercise. Both groups received dorsal wound operation with free access to food and water. By two-week continuous observation and recording of the wound area, the healing rate was analyzed...
February 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
Jörn F Dopheide, Philipp Geissler, Jennifer Rubrech, Amelie Trumpp, Geraldine C Zeller, Andreas Daiber, Thomas Münzel, Markus P Radsak, Christine Espinola-Klein
BACKGROUND: Inflammation is the driving force in atherosclerosis. One central strategy in the treatment of peripheral arterial disease (PAD) is the promotion of angiogenesis. Here, proangiogenic Tie-2 expressing monocytes (TEM) and circulating angiogenic cells (CAC) play a crucial role. Exercise training (ET) is recommended in PAD patients at Fontaine stage II to promote angiogenesis. METHODS: 40 patients with intermittend claudication (IC) [2 groups: supervised ET (SET) vs...
August 2016: Clinical Research in Cardiology: Official Journal of the German Cardiac Society
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