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Flow cytometry and exercise

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https://www.readbyqxmd.com/read/28183821/exercise-induced-upregulation-of-endothelial-adhesion-molecules-in-human-skeletal-muscle-and-number-of-circulating-cells-with-remodeling-properties
#1
Anna Strömberg, Eric Rullman, Eva Jansson, Thomas Gustafsson
Multipotent cells have received great interest due to their potential capacity to repair and remodel peripheral tissues. We examined the effect of an acute exercise bout on the number of circulating cells with known remodeling properties, and the level of factors in plasma and skeletal muscle tissue with potential to recruit these cells. Twenty healthy male subjects performed a 60 min cycling exercise. Blood samples for flow cytometry were drawn from 10 subjects (Group 1) before and up to 2 h after exercise, and absolute cell counts of the classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(++)) monocyte (MO) subpopulations, and of CD45(dim)CD34(+)VEGFR2(+) endothelial progenitor cells (EPCs) were measured using bead-based determination...
February 9, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28122717/acute-high-intensity-interval-exercise-reduces-human-monocyte-toll-like-receptor-2-expression-in-type-2-diabetes
#2
Cody Durrer, Monique Emily Francois, Helena Neudorf, Jonathan Peter Little
: Type 2 diabetes (T2D) is characterized by chronic low-grade inflammation that contributes to disease pathophysiology. Exercise has anti-inflammatory effects but the impact of high-intensity interval training (HIIT) is not known. PURPOSE: To determine the impact of a single session of HIIT on cellular, molecular, and circulating markers of inflammation in individuals with T2D. METHODS: Participants with T2D (n=10) and healthy age-matched controls (HC; n=9) completed an acute bout of HIIT (7 X 1-min @ ~85% maximal aerobic power output, separated by 1-min recovery) on a cycle ergometer with blood samples obtained before (Pre), immediately after (Post), and at one-hour of recovery (1-h Post)...
January 25, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28087817/t-cell-redeployment-and-intracellular-cytokine-expression-following-exercise-effects-of-exercise-intensity-and-cytomegalovirus-infection
#3
Emily C LaVoy, Maryam Hussain, Justin Reed, Hawley Kunz, Mira Pistillo, Austin B Bigley, Richard J Simpson
The magnitude of lymphocytosis following exercise is directly related to exercise intensity. Infection with cytomegalovirus (CMV) also augments lymphocytosis after exercise. It is not known if the enhanced T-cell response to exercise due to CMV depends on exercise intensity. Furthermore, exercise-induced changes in T-cell expression of type I and type II cytokines are thought to be intensity dependent, but direct comparisons are lacking. The aim of this experiment was to determine if CMV affects the exercise-induced redistribution of T-cell subsets at varying intensities, and determine the effect of exercise intensity on CD8(+) T-cell cytokine expression...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28039406/the-effect-of-polyphenols-on-cytokine-and-granulocyte-response-to-resistance-exercise
#4
Adam R Jajtner, Jay R Hoffman, Jeremy R Townsend, Kyle S Beyer, Alyssa N Varanoske, David D Church, Leonardo P Oliveira, Kelli A Herrlinger, Shlomit Radom-Aizik, David H Fukuda, Jeffrey R Stout
This study examined the effect of resistance exercise on the production, recruitment, percentage, and adhesion characteristics of granulocytes with and without polyphenol (PPB) supplementation. Thirty-eight untrained men were randomized into three groups: PPB (n = 13, 21.8 ± 2.5 years, 171.2 ± 5.5 cm, 71.2 ± 8.2 kg), placebo (PL; n = 15, 21.6 ± 2.5 years, 176.5 ± 4.9 cm, 84.0 ± 15.7 kg), or control (CON; n = 10, 23.3 ± 4.3 years, 173.7 ± 12.6 cm, 77.3 ± 16.3 kg). Blood samples were obtained pre (PRE), immediately (IP), 1 h (1H), 5 h (5H), 24 h (24H), 48 h (48H), and 96 h (96H) postresistance exercise (PPB/PL) or rest (CON)...
December 2016: Physiological Reports
https://www.readbyqxmd.com/read/28012941/age-related-arterial-immune-cell-infiltration-in-mice-is-attenuated-by-caloric-restriction-or-voluntary-exercise
#5
Daniel W Trott, Grant D Henson, Mi H T Ho, Sheilah A Allison, Lisa A Lesniewski, Anthony J Donato
Age-related arterial inflammation is associated with dysfunction of the arteries and increased risk for cardiovascular disease. To determine if aging increases arterial immune cell infiltration as well as the populations of immune cells principally involved, we tested the hypothesis that large elastic and resistance arteries in old mice would exhibit increased immune cell infiltration compared to young controls. Additionally, we hypothesized that vasoprotective lifestyle interventions such as lifelong caloric restriction or 8weeks of voluntary wheel running would attenuate age-related arterial immune cell infiltration...
December 22, 2016: Experimental Gerontology
https://www.readbyqxmd.com/read/28011264/inflammation-and-exercise-inhibition-of-monocytic-intracellular-tnf-production-by-acute-exercise-via-%C3%AE-2-adrenergic-activation
#6
Stoyan Dimitrov, Elaine Hulteng, Suzi Hong
Regular exercise is shown to exert anti-inflammatory effects, yet the effects of acute exercise on cellular inflammatory responses and its mechanisms remain unclear. We tested the hypothesis that sympathoadrenergic activation during a single bout of exercise has a suppressive effect on monocytic cytokine production mediated by β2 adrenergic receptors (AR). We investigated the effects of 20-min moderate (65-70% VO2 peak) exercise-induced catecholamine production on LPS-stimulated TNF production by monocytes in 47 healthy volunteers and determined AR subtypes involved...
December 21, 2016: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/27974950/evidence-of-a-redox-dependent-regulation-of-immune-responses-to-exercise-induced-inflammation
#7
Alexandra Sakelliou, Ioannis G Fatouros, Ioannis Athanailidis, Dimitrios Tsoukas, Athanasios Chatzinikolaou, Dimitris Draganidis, Athanasios Z Jamurtas, Christina Liacos, Ioannis Papassotiriou, Dimitrios Mandalidis, Kimon Stamatelopoulos, Meletios A Dimopoulos, Asimina Mitrakou
We used thiol-based antioxidant supplementation (n-acetylcysteine, NAC) to determine whether immune mobilisation following skeletal muscle microtrauma induced by exercise is redox-sensitive in healthy humans. According to a two-trial, double-blind, crossover, repeated measures design, 10 young men received either placebo or NAC (20 mg/kg/day) immediately after a muscle-damaging exercise protocol (300 eccentric contractions) and for eight consecutive days. Blood sampling and performance assessments were performed before exercise, after exercise, and daily throughout recovery...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27891564/a-homozygous-pign-missense-mutation-in-soft-coated-wheaten-terriers-with-a-canine-paroxysmal-dyskinesia
#8
Ana L Kolicheski, Gary S Johnson, Tendai Mhlanga-Mutangadura, Jeremy F Taylor, Robert D Schnabel, Taroh Kinoshita, Yoshiko Murakami, Dennis P O'Brien
Hereditary paroxysmal dyskinesias (PxD) are a heterogeneous group of movement disorders classified by frequency, duration, and triggers of the episodes. A young-adult onset canine PxD has segregated as an autosomal recessive trait in Soft-Coated Wheaten Terriers. The medical records and videos of episodes from 25 affected dogs were reviewed. The episodes of hyperkinesia and dystonia lasted from several minutes to several hours and could occur as often as >10/day. They were not associated with strenuous exercise or fasting but were sometimes triggered by excitement...
January 2017: Neurogenetics
https://www.readbyqxmd.com/read/27881669/aerobic-exercise-in-humans-mobilizes-hscs-in-an-intensity-dependent-manner
#9
Jeff M Baker, Joshua P Nederveen, Gianni Parise
: Hematopoietic stem and progenitor cells are necessary to maintain, repair, and reconstitute the hematopoietic blood cell system. Mobilization of these cells from bone marrow to blood can be greatly increased under certain conditions, one such being exercise. The purpose of this study was to identify the importance of exercise intensity in hematopoietic mobilization, to better understand the mobilization kinetics postexercise, and to determine if exercise is capable of mobilizing several specific populations of hematopoietic cells that have clinical relevance in a transplant setting...
January 1, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27865192/effect-of-targeted-therapy-on-circulating-progenitor-cells-in-precapillary-pulmonary-hypertension
#10
Jéssica García-Lucio, Olga Tura-Ceide, Roberto Del Pozo, Isabel Blanco, Sandra Pizarro, Elisabet Ferrer, Marta Díez, Núria Coll-Bonfill, Lucilla Piccari, Víctor I Peinado, Joan Albert Barberà
BACKGROUND: Endothelial dysfunction is key in the development of pulmonary hypertension (PH) and is associated with reduced number of circulating progenitor cells. Studies to date evaluating levels of circulating progenitor cells in PH have provided conflicting results. Current treatment of pulmonary arterial hypertension (PAH) and medical treatment of chronic thromboembolic pulmonary hypertension (CTEPH) targets endothelium dependent signalling pathways. The effect of PAH-targeted therapy on circulating progenitor cells has not been clearly established...
February 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/27815899/dna-damage-response-resulting-from-replication-stress-induced-by-synchronization-of-cells-by-inhibitors-of-dna-replication-analysis-by-flow-cytometry
#11
Dorota Halicka, Hong Zhao, Jiangwei Li, Jorge Garcia, Monika Podhorecka, Zbigniew Darzynkiewicz
Cell synchronization is often achieved by transient inhibition of DNA replication. When cultured in the presence of such inhibitors as hydroxyurea, aphidicolin or excess of thymidine the cells that become arrested at the entrance to S-phase upon release from the block initiate progression through S then G2 and M. However, exposure to these inhibitors at concentrations commonly used to synchronize cells leads to activation of ATR and ATM protein kinases as well as phosphorylation of Ser139 of histone H2AX. This observation of DNA damage signaling implies that synchronization of cells by these inhibitors is inducing replication stress...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27684595/measuring-granulocyte-and-monocyte-phagocytosis-and-oxidative-burst-activity-in-human-blood
#12
Mary Pat Meaney, David C Nieman, Dru A Henson, Qi Jiang, Fu-Zhang Wang
The granulocyte and monocyte phagocytosis and oxidative burst (OB) activity assay can be used to study the innate immune system. This manuscript provides the necessary methodology to add this assay to an exercise immunology arsenal. The first step in this assay is to prepare two aliquots ("H" and "F") of whole blood (heparin). Then, dihydroethidium is added to the H aliquot, and both aliquots are incubated in a warm water bath followed by a cold water bath. Next, Staphylococcus aureus (S. aureus) is added to the H aliquot and fluorescein isothiocyanate-labeled S...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27545396/exercise-does-not-increase-salivary-lymphocytes-monocytes-or-granulocytes-but-does-increase-salivary-lysozyme
#13
Trevor Gillum, Matthew Kuennen, Zachary McKenna, Micaela Castillo, Alex Jordan-Patterson, Caitlin Bohnert
An increase in salivary leukocytes may contribute to the exercise-induced increase in salivary antimicrobial proteins (AMPs). However, exercise-induced changes in salivary leukocytes have not been studied. The purpose of the study was to describe salivary leukocyte changes with exercise. Participants (n = 11, 20.3 ± 0.8 years, 57.2 ± 7.6 ml kg(-1) min(-1) peak oxygen uptake ((VO) ̇2peak), 11.1 ± 3.9% body fat) ran for 45 min at 75% of VO2peak. Stimulated saliva (12 mL) was collected pre- and immediately post exercise...
August 22, 2016: Journal of Sports Sciences
https://www.readbyqxmd.com/read/27525377/exercise-affects-t-cell-function-by-modifying-intracellular-calcium-homeostasis
#14
Renyi Liu, Wei Fan, Karsten Krüger, Y U Xiao, Christian Pilat, Michael Seimetz, Robert Ringseis, Eveline Baumgart-Vogt, Klaus Eder, Norbert Weissmann, Frank-Christoph Mooren
PURPOSE: The study aimed to investigate the effects of chronic moderate exercise on regulation of intracellular calcium signaling as an important link to proliferation capacity in murine splenic T lymphocytes. METHODS: Male CD1 Swiss mice were randomly assigned either to a control group (CG) or an exercise training group (EG). EG mice performed voluntary exercise for 3 months. Lymphocytes were isolated from murine spleens and intracellular calcium was determined by using Fura-2(AM) and fluorescence spectrometry...
January 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27427499/a-10-km-time-trial-running-bout-acutely-increases-the-number-of-angiogenic-t-cells-in-the-peripheral-blood-compartment-of-healthy-males
#15
Mark Ross, Peter Tormey, Lesley Ingram, Richard Simpson, Eva Malone, Geraint Florida-James
CD31(+) T-cells have been documented to possess vasculogenic properties, and have been termed 'angiogenic T-cells' (TANG ). No study to date has fully characterised the effect of acute exercise on TANG cells. Twelve male participants aged 24-45yrs undertook a running 10 km time trial, with peripheral blood samples taken pre-, immediately post- and 1-hour post-exercise for quantification of TANG cells and subsequent CXCR4 cell surface expression by flow cytometry. TANG cells demonstrated a 102% increase in number in peripheral circulation immediately post-exercise compared to pre-exercise levels, followed by a large egress (50%) from the circulation in total TANG cells 1-hour post-exercise...
July 18, 2016: Experimental Physiology
https://www.readbyqxmd.com/read/27387290/heterogeneous-circulating-angiogenic-cell-responses-to-acute-maximal-exercise
#16
Daniel D Shill, Meagan P Marshburn, Hannah K Hempel, Kasey A Lansford, Nathan T Jenkins
PURPOSE: Circulating angiogenic cells (CACs) comprise multiple subpopulations of exercise-inducible peripheral blood mononuclear cells (PBMCs) that promote angiogenesis and maintain endothelial integrity. We examined the effect of acute maximal exercise on CD31, CD62E, CD14/CD31, CD34/VEGFR2, CD3/CD31, and CD3 PBMCs in young, healthy adults. METHODS: Blood samples were collected before and immediately after a graded treadmill exercise test for CAC analysis via flow cytometry...
July 6, 2016: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27306382/comparison-of-capillary-and-venous-blood-in-the-analysis-of-concentration-and-function-of-leucocyte-sub-populations
#17
COMPARATIVE STUDY
Elisa F D Canetti, J Keane, C P McLellan, A B Gray
PURPOSE: Compare capillary and venous blood in the analysis of concentration and function of leucocyte sub-populations. This study hypothesised that capillary samples may be used in a site-specific manner as an alternative source of blood samples for assays of leucocyte concentration and neutrophilic phagocytic function and reactive oxygen species (ROS) production, allowing acquisition of multiple samples to better monitor transient but significant post-exercise immune modulation. METHODS: Resting blood samples were simultaneously obtained from vein, finger and earlobe of healthy subjects (n = 10, age: 25...
August 2016: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/27197857/exercise-induced-endothelial-progenitor-cell-mobilization-is-attenuated-in-impaired-glucose-tolerance-and-type-2-diabetes
#18
Andrew H Lutz, Jacob B Blumenthal, Rian Q Landers-Ramos, Steven J Prior
Circulating endothelial progenitor cells (EPCs) contribute to vascular homeostasis and are fewer in those with type 2 diabetes mellitus (T2DM) compared with normal glucose tolerance (NGT), suggesting a link between EPCs and T2DM-associated vasculopathies. The purpose of this study was to assess EPC number and mobilization by acute submaximal exercise in older adults with NGT, impaired glucose tolerance (IGT) or T2DM. We tested the hypothesis that EPC mobilization is lower in IGT compared with NGT and further reduced in older adults with T2DM...
July 1, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27183117/apoptosis-of-t-cell-subsets-after-acute-high-intensity-interval-exercise
#19
Karsten Krüger, Katharina Alack, Robert Ringseis, Lena Mink, Elisabeth Pfeifer, Matthias Schinle, Katharina Gindler, Lena Kimmelmann, Rüdiger Walscheid, Kerstin Muders, Torsten Frech, Klaus Eder, Frank-Christoph Mooren
INTRODUCTION: High-intensity interval training (HIT) exercise has gained much interest in both performance and recreational sports. This study aims to compare the effect of HIT versus continuous (CONT) exercise with regard to changes of circulating T cells and progenitor cells. METHODS: Subjects (n = 23) completed an HIT test and an isocaloric CONT test. Blood samples were collected before, immediately after, and 3 and 24 h postexercise for the assessment of low differentiated (CD3CD28CD57), highly differentiated T cells (CD3CD28CD57), regulatory T cells (Tregs) (CD4CD25CD127), hematopoietic progenitor cells (CD45CD34), and endothelial progenitor cells (CD45CD34KDR) by flow cytometry...
October 2016: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27028294/immune-cell-changes-in-response-to-a-swimming-training-session-during-a-24-h-recovery-period
#20
José P Morgado, Cristina P Monteiro, Júlia Teles, Joana F Reis, Catarina Matias, Maria T Seixas, Marta G Alvim, Mafalda Bourbon, Maria J Laires, Francisco Alves
Understanding the impact of training sessions on the immune response is crucial for the adequate periodization of training, to prevent both a negative influence on health and a performance impairment of the athlete. This study evaluated acute systemic immune cell changes in response to an actual swimming session, during a 24-h recovery period, controlling for sex, menstrual cycle phases, maturity, and age group. Competitive swimmers (30 females, 15 ± 1.3 years old; and 35 males, 16.5 ± 2.1 years old) performed a high-intensity training session...
May 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
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