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Flow cytometry and exercise

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https://www.readbyqxmd.com/read/28888922/microrna-20a-participates-in-the-aerobic-exercise-based-prevention-of-coronary-artery-disease-by-targeting-pten
#1
Dongwei Wang, Yi Wang, Juan Ma, Wenping Wang, Bingbing Sun, Tengfei Zheng, Ming Wei, Yangli Sun
BACKGROUND: Exercise can reduce the coronary artery disease (CAD) incidence. MiRNA-20a has been reported to distinctly expressed after sustain physical activity. However, its expression and regulation pattern in CAD model with or without exercise has not been reported. OBJECTIVE: In present study, we aim to investigate regulatory mechanism of miR-20a in exercise-associated reduced-CAD incidence and miR-20a-dependent signaling pathways. METHODS: Eight weeks old male ApoE/LDLR double knock out mice were recruited for this study...
September 7, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28864849/maximal-exercise-increases-mucosal-associated-invariant-t-cell-frequency-and-number-in-healthy-young-men
#2
Erik D Hanson, Eli Danson, Catriona V Nguyen-Robertson, Jackson J Fyfe, Nigel K Stepto, David B Bartlett, Samy Sakkal
PURPOSE: Mucosal associated invariant T (MAIT) cells have properties of the innate and acquired immune systems. While the response to vigorous exercise has been established for most leukocytes, MAIT cells have not been investigated. Therefore, the purpose was to determine if MAIT cell lymphocytosis occurs with acute maximal aerobic exercise and if this response is influenced by exercise duration, cardiovascular fitness, or body composition. METHODS: Twenty healthy young males with moderate fitness levels performed an extended graded exercise test until volitional fatigue...
September 1, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28828264/exercise-training-with-dietary-restriction-enhances-circulating-irisin-level-associated-with-increasing-endothelial-progenitor-cell-number-in-obese-adults-an-intervention-study
#3
Junhao Huang, Shen Wang, Fengpeng Xu, Dan Wang, Honggang Yin, Qinhao Lai, Jingwen Liao, Xiaohui Hou, Min Hu
OBJECTIVE: Circulating endothelial progenitor cells (EPCs) correlate negatively with obesity. Previous studies have shown that exercise significantly restores circulating EPC levels in obese people; however, the underlying mechanisms have not been elucidated. Recently, irisin has been reported to have a critical role in the regulation of EPCs. This exercise-induced myokine has been demonstrated to play a therapeutic role in obesity. In this study, we hypothesized that the increase in circulating irisin may form a link with increasing EPC levels in obese people after exercise...
2017: PeerJ
https://www.readbyqxmd.com/read/28817660/cited4-is-related-to-cardiogenic-induction-and-maintenance-of-proliferation-capacity-of-embryonic-stem-cell-derived-cardiomyocytes-during-in-vitro-cardiogenesis
#4
Junichiro Miake, Tomomi Notsu, Katsumi Higaki, Kyoko Hidaka, Takayuki Morisaki, Kazuhiro Yamamoto, Ichiro Hisatome
Cardiac progenitor cells have a limited proliferative capacity. The CREB-binding protein/p300-interacting transactivator, with the Glu/Asp-rich carboxy-terminal domain (Cited) gene family, regulates gene transcription. Increased expression of the Cited4 gene in an adult mouse is associated with exercise-induced cardiomyocyte hypertrophy and proliferation. However, the expression patterns and functional roles of the Cited4 gene during cardiogenesis are largely unknown. Therefore, in the present study, we investigated the expression patterns and functional roles of the Cited4 gene during in vitro cardiogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28806276/circulating-progenitor-cell-response-to-exercise-in-wheelchair-racing-athletes
#5
Grace M Niemiro, Thomas Edwards, J P Barfield, Joseph W Beals, Elizabeth M Broad, Robert W Motl, Nicholas A Burd, Lara A Pilutti, Michael De Lisio
INTRODUCTION: Circulating progenitor cells (CPCs) are a heterogeneous population of stem/progenitor cells in peripheral blood that participate in tissue repair. CPC mobilization has been well characterized in able-bodied persons, but has not been previously investigated in wheelchair racing athletes. The purpose of this study was to characterize CPC and CPC sub-population mobilization in elite wheelchair racing athletes in response to acute, upper-extremity aerobic exercise to determine if CPC responses are similar to ambulatory populations...
August 11, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28796240/glucocorticoid-receptor-expression-on-circulating-leukocytes-in-healthy-and-asthmatic-adolescents-in-response-to-exercise
#6
Kim D Lu, Dan Cooper, Fadia Haddad, Frank Zaldivar, Monica Kraft, Shlomit Radom-Aizik
BackgroundPoor aerobic fitness is associated with worsening of asthma symptoms, and fitness training may improve asthma control. The mechanism linking fitness with asthma is not known. We hypothesized that repeated bouts of exercise would lead to a downregulation of glucocorticoid receptor (GR) expression on circulating leukocytes, reflecting a reduced responsiveness to stress.MethodsIn a prospective exercise training intervention of healthy and asthmatic adolescents, GR expression in leukocytes was measured using flow cytometry in response to an acute exercise challenge before and after the exercise training intervention...
August 2017: Pediatric Research
https://www.readbyqxmd.com/read/28767980/bone-marrow-cell-migration-to-the-heart-in-a-chimeric-mouse-model-of-acute-chagasic-disease
#7
Camila Iansen Irion, Bruno Diaz Paredes, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, Luis Felipe Paula, Alysson Roncally Carvalho, Antonio Carlos Campos de Carvalho, Adriana Bastos Carvalho, Regina Coeli Dos Santos Goldenberg
BACKGROUND: Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28683357/exercise-improves-cytokine-profile-in-hiv-infected-people-a-randomized-clinical-trial
#8
Rafael Evangelista Pedro, Nadia Candido, Débora Alves Guariglia, Bruno Pereira Melo, Dennis Armando Bertolini, Sidney Barnabé Peres, Solange Marta Franzói de Moraes
PURPOSE: Verify the effects of concurrent training on cytokines in people living with HIV under antiretroviral therapy (ART) treatment. METHODS: This was a blinded, parallel-group, clinical trial, where 49 participants, divided in two groups, either control group or concurrent training group, took part in the intervention. The control group performed recreational activities and concurrent training group participated of 16-week, 3 times per week of heart rate guided-aerobic plus resistance training for major muscular groups...
July 3, 2017: Cytokine
https://www.readbyqxmd.com/read/28668892/measurement-of-the-phagocytic-activity-of-human-peripheral-blood-using-a-highly-sensitive-fluorometric-detection-device-without-hemolysis
#9
Ran Zhang, Hiroyuki Inagawa, Masaru Takahashi, Hisami Kawanishi, Kimiko Kazumura, Hiroshi Tsuchiya, Naokazu Morishita, Yutaro Kobayashi, Tsutomu Masaki, Hideki Kobara, Gen-Ichiro Soma
BACKGROUND/AIM: Phagocytes recognize pathogens that enter the body as well as other abnormal and foreign materials that may exist within an organism (such as dead cells, oxidized lipids, and denatured proteins), and phagocytose and eliminate them to maintain a healthy state. In a previous study a simple prototype device was used, under development by Hamamatsu Photonics (Prototype), that detects fluorescence to determine the phagocytic activity of the murine macrophage cell line J774...
July 2017: Anticancer Research
https://www.readbyqxmd.com/read/28646302/t-regulatory-cells-exhibit-a-biphasic-response-to-prolonged-endurance-exercise-in-humans
#10
Tom Clifford, Matthew J Wood, Philip Stocks, Glyn Howatson, Emma J Stevenson, Catharien M U Hilkens
PURPOSE: T-regulatory cells (Tregs) are a sub-population of lymphocytes that act to suppress aberrant immune responses. We investigated changes in the numbers of naïve and terminally differentiated Tregs in the peripheral blood to establish their role in the immuno-suppressive response to prolonged exercise. METHODS: Blood was drawn from seventeen experienced runners (age 40 ± 12 years; height 1.75 ± 0.08 m; mass 71.4 ± 10.8 kg) before, ~1 h after (POST-1h), and on the day following the marathon (POST-1d)...
August 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28554920/physical-activity-immune-function-and-inflammation-in-kidney-patients-the-pink-study-a-feasibility-trial-protocol
#11
Patrick James Highton, Jill Neale, Thomas J Wilkinson, Nicolette C Bishop, Alice C Smith
INTRODUCTION: Patients with chronic kidney disease (CKD) display increased infection-related mortality and elevated cardiovascular risk only partly attributed to traditional risk factors. Patients with CKD also exhibit a pro-inflammatory environment and impaired immune function. Aerobic exercise has the potential to positively impact these detriments, but is under-researched in this patient population. This feasibility study will investigate the effects of acute aerobic exercise on inflammation and immune function in patients with CKD to inform the design of larger studies intended to ultimately influence current exercise recommendations...
May 29, 2017: BMJ Open
https://www.readbyqxmd.com/read/28534118/exercise-induced-circulating-extracellular-vesicles-protect-against-cardiac-ischemia-reperfusion-injury
#12
Yihua Bei, Tianzhao Xu, Dongchao Lv, Pujiao Yu, Jiahong Xu, Lin Che, Avash Das, John Tigges, Vassilios Toxavidis, Ionita Ghiran, Ravi Shah, Yongqin Li, Yuhui Zhang, Saumya Das, Junjie Xiao
Extracellular vesicles (EVs) serve an important function as mediators of intercellular communication. Exercise is protective for the heart, although the signaling mechanisms that mediate this cardioprotection have not been fully elucidated. Here using nano-flow cytometry, we found a rapid increase in plasma EVs in human subjects undergoing exercise stress testing. We subsequently identified that serum EVs were increased by ~1.85-fold in mice after 3-week swimming. Intramyocardial injection of equivalent quantities of EVs from exercised mice and non-exercised controls provided similar protective effects against acute ischemia/reperfusion (I/R) injury in mice...
July 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28474171/disruption-of-the-aldehyde-dehydrogenase-2-gene-results-in-no-increase-in-trabecular-bone-mass-due-to-skeletal-loading-in-association-with-impaired-cell-cycle-regulation-through-p21-expression-in-the-bone-marrow-cells-of-mice
#13
Kayoko Furukawa Okuma, Kunitaka Menuki, Manabu Tsukamoto, Takafumi Tajima, Hokuto Fukuda, Yasuaki Okada, Toshiharu Mori, Takuto Tsuchiya, Toshihiro Kawamoto, Yasuhiro Yoshida, Soshi Uchida, Akinori Sakai
Approximately 45% of people of East Asian descent have the inactive aldehyde dehydrogenase 2 (ALDH2) phenotype. The enzyme defect of ALDH2 has been found to adversely influence the risk of osteoporosis. The aim of this study was to clarify the effect of skeletal loading on trabecular bone structure and dynamics in Aldh2-disrupted mice in the absence of alcohol consumption. Four-week-old male Aldh2(-/-) (KO) and Aldh2(+/+) (WT) mice were divided into a ground control (GC) group and a climbing exercise (CE) group in each genotype...
September 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28443964/effects-of-chronic-exercise-on-endothelial-progenitor-cells-and-microparticles-in-professional-runners
#14
COMPARATIVE STUDY
Célia Regina de Oliveira Bittencourt, Maria Cristina de Oliveira Izar, Carolina Nunes França, Valdir Lauro Schwerz, Rui Manuel Dos Santos Póvoa, Francisco Antonio Helfenstein Fonseca
BACKGROUND: The effects of chronic exposure to exercise training on vascular biomarkers have been poorly explored. OBJECTIVE: Our study aimed to compare the amounts of endothelial progenitor cells (EPCs), and endothelial (EMP) and platelet (PMP) microparticles between professional runners and healthy controls. METHODS: Twenty-five half-marathon runners and 24 age- and gender-matched healthy controls were included in the study. EPCs (CD34+/KDR+, CD133+/KDR+, and CD34+/CD133+), EMP (CD51+) and PMP (CD42+/CD31+) were quantified by flow-cytometry...
March 2017: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28428879/a-systems-biology-approach-to-investigating-the-influence-of-exercise-and-fitness-on-the-composition-of-leukocytes-in-peripheral-blood
#15
Michael P Gustafson, Ara Celi DiCostanzo, Courtney M Wheatley, Chul-Ho Kim, Svetlana Bornschlegl, Dennis A Gastineau, Bruce D Johnson, Allan B Dietz
BACKGROUND: Exercise immunology has become a growing field in the past 20 years, with an emphasis on understanding how different forms of exercise affect immune function. Mechanistic studies are beginning to shed light on how exercise may impair the development of cancer or be used to augment cancer treatment. The beneficial effects of exercise on the immune system may be exploited to improve patient responses to cancer immunotherapy. METHODS: We investigated the effects of acute exercise on the composition of peripheral blood leukocytes over time in a male population of varying fitness...
2017: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/28418842/effects-of-tlr-2-nf-%C3%AE%C2%BAb-signaling-pathway-on-the-occurrence-of-degenerative-knee-osteoarthritis-an-in-vivo-and-in-vitro-study
#16
Yi-Xun Liu, Guo-Dong Wang, Xiao Wang, Yong-Le Zhang, Tian-Lun Zhang
The study aims to explore the effects of TLR-2/NF-κB signaling pathway on the occurrence of degenerative knee osteoarthritis (OA). Degenerative knee OA and normal cartilage samples were collected from patients with degenerative knee OA receiving total knee arthroplasty and amputation. Expressions of TLR-2, NF-κB and MMP-13 were determined by qRT-PCR and immunochemistry. The chondrocytes were divided into control, IL-1β, IL-1β + anti-TLR-2 and IL-1β + PDTC groups. MTT assay and flow cytometry were performed to determine proliferation and apoptosis of the chondrocytes...
June 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28341903/exercise-increases-lactoferrin-but-decreases-lysozyme-in-salivary-granulocytes
#17
Trevor Gillum, Matthew Kuennen, Zachary McKenna, Micaela Castillo, Alex Jordan-Patterson, Caitlin Bohnert
INTRODUCTION: Intracellular lactoferrin (Lac) and lysozyme (Lys) content play an important role in regulating inflammation and promoting host protection. While exercise has demonstrated an increase in Lac and Lys concentration in exocrine solutions, little is known regarding intracellular concentration changes in response to exercise. PURPOSE: To quantify intracellular Lac and Lys concentration before and after exercise in salivary CD45(+)CD15(+) cells. METHODS: 11 males (20...
May 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28250797/changes-in-peripheral-immune-cell-numbers-and-functions-in-octogenarian-walkers-an-acute-exercise-study
#18
Kornelis S M van der Geest, Qi Wang, Thijs M H Eijsvogels, Hans J P Koenen, Irma Joosten, Elisabeth Brouwer, Maria T E Hopman, Joannes F M Jacobs, Annemieke M H Boots
BACKGROUND: Age-related changes of the immune system, termed immunosenescence, may underlie the increased risk of infections and morbidity in the elderly. Little is known about the effects of acute exercise on peripheral immune parameters in octogenarians. Therefore, we investigated acute exercise-induced changes in phenotype and function of the immune system in octogenarians participating in the 2013 edition of the Nijmegen Four Days Marches. Blood sampling was performed at baseline and immediately after 4 days of the walking exercise (30 km/day)...
2017: Immunity & Ageing: I & A
https://www.readbyqxmd.com/read/28183821/exercise-induced-upregulation-of-endothelial-adhesion-molecules-in-human-skeletal-muscle-and-number-of-circulating-cells-with-remodeling-properties
#19
Anna Strömberg, Eric Rullman, Eva Jansson, Thomas Gustafsson
Multipotent cells have received great interest because of their potential capacity to repair and remodel peripheral tissues. We examined the effect of an acute exercise bout on the number of circulating cells with known remodeling properties and the level of factors in plasma and skeletal muscle tissue with potential to recruit these cells. Twenty healthy male subjects performed a 60-min cycling exercise. Blood samples for flow cytometry were drawn from 10 subjects (group 1) before and up to 2 h after exercise, and absolute cell counts of the classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(++)) monocyte (MO) subpopulations and of CD45(dim)CD34(+)VEGFR2(+) endothelial progenitor cells (EPCs) were measured by bead-based determination...
May 1, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28122717/acute-high-intensity-interval-exercise-reduces-human-monocyte-toll-like-receptor-2-expression-in-type-2-diabetes
#20
Cody Durrer, Monique Francois, Helena Neudorf, Jonathan P Little
Type 2 diabetes (T2D) is characterized by chronic low-grade inflammation that contributes to disease pathophysiology. Exercise has anti-inflammatory effects, but the impact of high-intensity interval training (HIIT) is not known. The purpose of this study was to determine the impact of a single session of HIIT on cellular, molecular, and circulating markers of inflammation in individuals with T2D. Participants with T2D (n = 10) and healthy age-matched controls (HC; n = 9) completed an acute bout of HIIT (7 × 1 min at ~85% maximal aerobic power output, separated by 1 min of recovery) on a cycle ergometer with blood samples obtained before (Pre), immediately after (Post), and at 1 h of recovery (1-h Post)...
April 1, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
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