keyword
https://read.qxmd.com/read/38493427/circrna-hlcs-regulates-lens-epithelial-cell-apoptosis-via-mir-338-3p-bpnt1-axis
#1
JOURNAL ARTICLE
Lianyi Sun, Fengzhi Li, Shuwei Bai, Chunchao Bi
PURPOSE: The purpose of this study was to investigate the effect of circ_HLCS on age-related cataract (ARC). METHODS: Circ_HLCS, microRNA (miR)-338-3p, and bisphosphate 3'-nucleotidase 1 (BPNT1) were quantified by quantitative real-time polymerase chain reaction or western blot. Cell proliferation and cell viability were assessed by the 5-Ethynyl-2'-deoxyuridinr and cell counting kit-8 assays. Cell apoptosis was detected by flow cytometry. Targeted correlations among circ_HLCS, miR-338-3p, and BPNT1 were verified by the dual-luciferase reporter and RNA pull-down assays...
March 17, 2024: International Ophthalmology
https://read.qxmd.com/read/38493325/amyloid-typing-in-cardiac-amyloidosis-using-western-blotting
#2
JOURNAL ARTICLE
Batia Kaplan, Rivka Goldis, Tamar Ziv, Amir Dori, Hila Magen, Amos J Simon, Alexander Volkov, Elad Maor, Michael Arad
BACKGROUND: Cardiac amyloidosis (CA) is characterized by the extracellular deposition of misfolded protein in the heart. Precise identification of the amyloid type is often challenging, but critical, since the treatment and prognosis depend on the disease form and the type of deposited amyloid. Coexistence of clinical conditions such as old age, monoclonal gammopathy, chronic inflammation, or peripheral neuropathy in a patient with cardiomyopathy creates a differential diagnosis between the major types of CA: amyloidosis light chains (AL), amyloidosis transthyretin (ATTR) and amyloidosis A (AA)...
March 2024: Israel Medical Association Journal: IMAJ
https://read.qxmd.com/read/38493281/impact-of-the-covid-19-pandemic-on-persons-living-with-hiv-in-western-washington-examining-lived-experiences-of-social-distancing-stress-personal-buffers-and-mental-health
#3
JOURNAL ARTICLE
Sarah Smith, Kristin Beima-Sofie, Asad Naveed, Nikki Bhatia, Murugi Micheni, Anh Tuyet Nguyen, Francis Slaughter, Liying Wang, Sandeep Prabhu, Stephaun Wallace, Jane Simoni, Susan M Graham
Pandemic-related stressors may disproportionately affect the mental health of people with HIV (PWH). Stratified, purposive sampling was used to recruit 24 PWH who participated in a quantitative survey on COVID-19 experiences for in-depth interviews (IDIs). IDIs were conducted by Zoom, audio recorded and transcribed. Thematic analysis was used to develop an adapted stress-coping model. Participants experienced acute stress following exposure events and symptoms compatible with COVID-19. Social isolation and job loss were longer-term stressors...
March 16, 2024: AIDS and Behavior
https://read.qxmd.com/read/38493128/single-cell-transcriptomic-sequencing-data-reveal-aberrant-dna-methylation-in-smad3-promoter-region-in-tumor-associated-fibroblasts-affecting-molecular-mechanism-of-radiosensitivity-in-non-small-cell-lung-cancer
#4
JOURNAL ARTICLE
Fushi Han, Shuzhen Chen, Kangwei Zhang, Kunming Zhang, Meng Wang, Peijun Wang
OBJECTIVE: Non-small cell lung cancer (NSCLC) often exhibits resistance to radiotherapy, posing significant treatment challenges. This study investigates the role of SMAD3 in NSCLC, focusing on its potential in influencing radiosensitivity via the ITGA6/PI3K/Akt pathway. METHODS: The study utilized gene expression data from the GEO database to identify differentially expressed genes related to radiotherapy resistance in NSCLC. Using the GSE37745 dataset, prognostic genes were identified through Cox regression and survival analysis...
March 16, 2024: Journal of Translational Medicine
https://read.qxmd.com/read/38493098/comparison-of-different-trapping-methods-to-collect-malaria-vectors-indoors-and-outdoors-in-western-kenya
#5
JOURNAL ARTICLE
Jackline Kosgei, John E Gimnig, Vincent Moshi, Seline Omondi, Daniel P McDermott, Martin J Donnelly, Collins Ouma, Bernard Abong'o, Eric Ochomo
BACKGROUND: Vector surveillance is among the World Health Organization global vector control response (2017-2030) pillars. Human landing catches are a gold standard but difficult to implement and potentially expose collectors to malaria infection. Other methods like light traps, pyrethrum spray catches and aspiration are less expensive and less risky to collectors. METHODS: Three mosquito sampling methods (UV light traps, CDC light traps and Prokopack aspiration) were evaluated against human landing catches (HLC) in two villages of Rarieda sub-county, Siaya County, Kenya...
March 16, 2024: Malaria Journal
https://read.qxmd.com/read/38493021/preclinical-efficacy-of-raf-mek-clamp-avutometinib-in-combination-with-fak-inhibition-in-low-grade-serous-ovarian-cancer
#6
JOURNAL ARTICLE
Blair McNamara, Cem Demirkiran, Tobias Max Philipp Hartwich, Stefania Bellone, Diego Manavella, Levent Mutlu, Michelle Greenman, Margherita Zipponi, Yang Yang-Hartwich, Kevin Yang, Elena Ratner, Peter E Schwartz, Silvia Coma, Jonathan A Pachter, Alessandro D Santin
OBJECTIVES: Low-grade-serous-ovarian-carcinoma (LGSOC) is characterized by a high recurrence rate and limited therapeutic options. About one-third of LGSOC contains mutations in MAPK pathway genes such as KRAS/NRAS/BRAF. Avutometinib is a dual RAF/MEK inhibitor while defactinib and VS-4718 are focal-adhesion-kinase-inhibitors (FAKi). We determined the preclinical efficacy of avutometinib±VS-4718 in LGSOC patient-derived-tumor-xenografts (PDX). METHODS: Whole-exome-sequencing (WES) was used to evaluate the genetic fingerprint of 3 patient-derived LGSOC (OVA(K)250, PERIT(M)17 and A(PE)148)...
February 9, 2024: Gynecologic Oncology
https://read.qxmd.com/read/38492983/inhibition-of-keloid-fibroblast-proliferation-by-artesunate-is-mediated-by-targeting-the-ire1%C3%AE-xbp1-signaling-pathway-and-decreasing-tgf-%C3%AE-1
#7
JOURNAL ARTICLE
Xiaomei Han, Shaoqian Jiang, Caixia Hu, Ying Wang, Lu Zhao, Wenqing Wang
BACKGROUND: Keloid is a benign hyperplastic dermatosis with high recurrence rate and complex pathogenesis. There is no universally effective treatment yet. New therapies and elucidation of pathogenesis are urgently required. AIMS: To explore the function of IRE1α/XBP1 in keloid fibroblasts and to investigate the potential mechanism of artesunate in inhibiting keloid hyperplasia. METHODS: Human keloid fibroblasts (KFs) were cultured, and the expressions of XBP1 and TGF-β1 were detected by immunohistochemistry...
March 5, 2024: Burns
https://read.qxmd.com/read/38492922/zebrafish-mutants-in-egln1-display-a-hypoxic-response-and-develop-polycythemia
#8
JOURNAL ARTICLE
Xuehan Dou, Zhongyuan Chen, Yunzhang Liu, Yun Li, Junli Ye, Ling Lu
AIMS: Prolyl hydroxylase domain 2 (PHD2), encoded by the Egln1 gene, serves as a pivotal regulator of the hypoxia-inducible factor (HIF) pathway and acts as a cellular oxygen sensor. Somatic inactivation of PHD2 in mice results in polycythemia and congestive heart failure. However, due to the embryonic lethality of PHD2 deficiency, its role in development remains elusive. Here, we investigated the function of two egln1 paralogous genes, egln1a and egln1b, in zebrafish. MAIN METHODS: The egln1 null zebrafish were generated using the CRISPR/Cas9 system...
March 14, 2024: Life Sciences
https://read.qxmd.com/read/38492912/melatonin-attenuates-fentanyl-induced-behavioral-sensitization-and-circadian-rhythm-disorders-in-mice
#9
JOURNAL ARTICLE
Kaili Du, Qianwen Shi, Xiuya Zhou, Lifei Zhang, Hongliang Su, Chao Zhang, Zhiwen Wei, Ting Liu, Li Wang, Xiaohui Wang, Bin Cong, Keming Yun
Melatonin is a neurohormone synthesized by the pineal gland to regulate the circadian rhythms and has proven to be effective in treating drug addiction and dependence. However, the effects of melatonin to modulate the drug-seeking behavior of fentanyl and its underlying molecular mechanism is elusive. This study was designed to investigate the effects of melatonin on fentanyl - induced behavioral sensitization and circadian rhythm disorders in mice. The accompanying changes in the expression of Brain and Muscle Arnt-Like (BMAL1), tyrosine hydroxylase (TH), and monoamine oxidase A (MAO-A) in relevant brain regions including the suprachiasmatic nucleus (SCN), nucleus accumbens (NAc), prefrontal cortex (PFC), and hippocampus (Hip) were investigated by western blot assays to dissect the mechanism by which melatonin modulates fentanyl - induced behavioral sensitization and circadian rhythm disorders...
March 14, 2024: Physiology & Behavior
https://read.qxmd.com/read/38492898/pterostilbene-nanoemulsion-promotes-nrf2-signaling-pathway-to-downregulate-oxidative-stress-for-treating-alzheimer-s-disease
#10
JOURNAL ARTICLE
Jingyu Liu, Jikai Xu, Luan Jia, Yanjun Zhou, Qiang Fu, Yichen Wang, Danyang Mu, Dequan Wang, Ning Li, Yue Hou
Pterostilbene, a stilbene compound, demonstrates neuroprotective effects through its antioxidant and anti-inflammatory properties. However, pterostilbene exhibits low bioavailability. We developed a pterostilbene nanoemulsion with better release stability and particle size. Behavioral tests, including the Y maze, new object recognition, and water maze, revealed that the pterostilbene nanoemulsion demonstrated a more significant effect on improving learning and memory function than pterostilbene. Immunofluorescence analysis revealed that pterostilbene nanoemulsion was more potent in safeguarding hippocampal neurons and inhibiting apoptosis and oxidative stress than pterostilbene...
March 14, 2024: International Journal of Pharmaceutics
https://read.qxmd.com/read/38492884/ginkgolide-b-can-alleviate-spinal-glymphatic-system-dysfunction-and-provide-neuroprotection-in-pdn-rats-by-inhibiting-matrix-metalloproteinase-9
#11
JOURNAL ARTICLE
Jiang Li, Shuaiying Jia, Yuehan Song, Wenmei Xu, Jingyan Lin
The glymphatic system plays a crucial role in maintaining optimal central nervous system (CNS) function by facilitating the removal of metabolic wastes. Aquaporin-4 (AQP4) protein, predominantly located on astrocyte end-feet, is a key pathway for metabolic waste excretion. β-Dystroglycan (β-DG) can anchor AQP4 protein to the end-feet membrane of astrocytes and can be cleaved by matrix metalloproteinase (MMP)-9 protein. Studies have demonstrated that hyperglycemia upregulates MMP-9 expression in the nervous system, leading to neuropathic pain...
March 14, 2024: Neuropharmacology
https://read.qxmd.com/read/38492883/pgc-1%C3%AE-in-the-hippocampus-mediates-depressive-like-and-stress-coping-behaviours-and-regulates-excitatory-synapses-in-the-dentate-gyrus-in-mice
#12
JOURNAL ARTICLE
Yuhui Deng, Xin Liang, Yue Li, Lin Jiang, Jin Wang, Jing Tang, Jing Li, Yuhan Xie, Kai Xiao, Peilin Zhu, Yijing Guo, Yanmin Luo, Yong Tang
Decreased hippocampal synaptic plasticity is an important pathological change in stress-related mood disorders, including major depressive disorder. However, the underlying mechanism is unclear. PGC-1α, a transcriptional coactivator, is a key factor in synaptic plasticity. We investigated the relationships between changes in hippocampal PGC-1α expression and depressive-like and stress-coping behaviours, and whether they are related to hippocampal synapses. Adeno-associated virus was used to alter hippocampal PGC-1α expression in male C57BL/6 mice...
March 14, 2024: Neuropharmacology
https://read.qxmd.com/read/38492821/mutational-profile-evaluates-metastatic-capacity-of-chinese-colorectal-cancer-patients-revealed-by-whole-exome-sequencing
#13
JOURNAL ARTICLE
Yian Yang, Jiawei Chen, Honghua Peng, Zhigang Xiao, Wei Xu, Mingchuan Zheng, Zheng Li, Peiguo Cao
Colorectal cancer (CRC) is the third most common cancer and the prevalence rate of CRC is increasing in the China. In this study, whole-exome sequencing (WES) was performed on primary tissues of 47 CRC Chinese patients including 22 metastatic and 25 non-metastatic patients. By comparison with data from western colorectal cancer patients in the Cancer Genome Atlas (TCGA), we identified a number of genes that are more likely to be mutated in Chinese colorectal cancer patients, such as MUC12, MUC12, MUC2, MUC4, HYDIN and KMT2C...
March 14, 2024: Genomics
https://read.qxmd.com/read/38492796/armcx1-reduces-neurological-damage-via-a-mitochondrial-transport-pathway-involving-miro1-after-traumatic-brain-injury
#14
JOURNAL ARTICLE
Qiuying Li, Haibo Ni, Qin Rui, Jiasheng Ding, Xianghu Kong, Xugang Kan, Rong Gao, Hongbo Shen
Armcx1 is a member of the ARMadillo repeat-Containing protein on the X chromosome (ARMCX) family, which is recognized to have evolutionary conserved roles in regulating mitochondrial transport and dynamics. Previous research has shown that Armcx1 is expressed at higher levels in mice after axotomy and in adult retinal ganglion cells after crush injury, and this protein increases neuronal survival and axonal regeneration. However, its role in traumatic brain injury (TBI) is unclear. Therefore, the aim of this study was to assess the expression of Armcx1 after TBI and to explore possible related mechanisms by which Armcx1 is involved in TBI...
March 14, 2024: Neuroscience
https://read.qxmd.com/read/38492791/deciphering-the-antidepressant-effects-of-rosa-damascena-essential-oil-mediated-through-the-serotonergic-synapse-signaling-pathway
#15
JOURNAL ARTICLE
Ning Xia, Jie Wang, Qiuting Guo, Jiawei Duan, Xuan Wang, Peijie Zhou, Jinkai Li, Tiantian Tang, Taotao Li, Huiting Li, Zhenfeng Wu, Ming Yang, Jing Sun, Dongyan Guo, Xing Chang, Xiaofei Zhang
ETHNOPHARMACOLOGICAL RELEVANCE: Rosa damascena is an ancient plant with significance in both medicine and perfumery that have a variety of therapeutic properties, including antidepressant, anti-anxiety, and anti-stress effects. Rose damascena essential oil (REO) has been used to treat depression, anxiety and other neurological related disorders in Iranian traditional medicine. However, its precise mechanism of action remains elusive. AIM OF THE STUDY: The aim of this study was to investigate the impact and mechanism underlying the influence of REO on chronic unpredictable mild stress (CUMS) rats...
March 14, 2024: Journal of Ethnopharmacology
https://read.qxmd.com/read/38492701/adipose-derived-stem-cells-loaded-photocurable-and-bioprintable-bioinks-composed-of-gelma-hama-and-pegda-crosslinker-to-differentiate-into-smooth-muscle-phenotype
#16
JOURNAL ARTICLE
Pavanchandh Atturu, Sunaina Mudigonda, Chau-Zen Wang, Shun-Cheng Wu, Jhen-Wei Chen, Mary Fornica Francis Forgia, Hans-Uwe Dahms, Chih-Kuang Wang
Developing a polymer-based photocrosslinked 3D printable scaffolds comprised of gelatin methacryloyl (G) and hyaluronic acid methacryloyl (H) incorporated with two molecular weights of polyethylene glycol diacrylate (P) of various concentrations that enables rabbit adipose-derived stem cells (rADSCs) to survive, grow, and differentiate into smooth muscle cells (SMCs). Then, the chemical modification and physicochemical properties of the PGH bioinks were evaluated. The cell viability was assessed via MTT, CCK-8 assay and visualized employing Live/Dead assay...
March 14, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38492656/remazolam-affects-the-phenotype-and-function-of-astrocytes-to-improve-traumatic-brain-injury-by-regulating-the-cx43
#17
JOURNAL ARTICLE
Jing Xia, Yang Tan, Congli Mao, Wenting Shen, Ying Zhao
PURPOSE: To explore the mechanism by which Remazolam affects the phenotype and function of astrocytes to improve traumatic brain injury (TBI). METHODS: The oxygen -glucose deprivation/recovery (OGD/R) cell model was constructed to simulate the pathological state of astrocytes in a TBI environment. The viability of astrocytes was measured by CCK-8, and the cytoskeleton changes were observed by Phalloidin- TRITC staining. The expressions of differentiation markers, Cx43 and phosphorylated Cx43 (P-Cx43) of A1/A2 astrocytes were detected by Western blot, and the complement C3 and S100A10 of A1/A2 astrocytes were detected by ELISA...
March 14, 2024: Experimental Gerontology
https://read.qxmd.com/read/38492650/antidepressant-like-effects-of-hyperin-on-chronic-stress-induced-depressive-like-behaviors-in-mice-gut-microbiota-and-short-chain-fatty-acids
#18
JOURNAL ARTICLE
Aoqi Song, Ru Cheng, Jingjing Jiang, Han Qu, Zhenghua Wu, Feng Qian, Shuyu Shen, Liwen Zhang, Zhiyu Wang, Wenjuan Zhao, Yuefen Lou
BACKGROUND: The antidepressant effect of hyperoside (HYP), which is the main component of Hypericum perforatum, is not established. This study aimed to determine the effects of HYP on depression. METHODS: The antidepressant-like effect of HYP was studied in mice induced by chronic restraint stress (CRS). The effects of HYP on behavior, inflammation, neurotransmitters, gut microbiota, and short-chain fatty acids (SCFAs) were studied in CRS mice. RESULTS: HYP improved depressive-like behavior in mice induced by CRS...
March 14, 2024: Journal of Affective Disorders
https://read.qxmd.com/read/38492634/notoginsenoside-r1-targets-ppar-%C3%AE-to-inhibit-hepatic-stellate-cell-activation-and-ameliorates-liver-fibrosis
#19
JOURNAL ARTICLE
Cheng Guo, Linying Lai, Boyu Ma, Qian Huang, Zhirong Wang
BACKGROUND: Hepatic fibrosis, a common pathological process that occurs in end-stage liver diseases, is a serious public health problem and lacks effective therapy. Notoginsenoside R1 (NR1) is a small molecule derived from the traditional Chinese medicine Sanqi, exhibiting great potential in treating diverse metabolie disorders. Here we aimed to enquired the role of NR1 in liver fibrosis and its underlying mechanism in hepatoprotective effects. METHODS: We investigated the anti-fibrosis effects of NR1 using CCl4 -induced mouse mode of liver fibrosis as well as TGF-β1-activated JS-1, LX-2 cells and primary hepatic stellate cells...
March 14, 2024: Experimental Cell Research
https://read.qxmd.com/read/38492609/identification-of-an-n-terminal-tag-580n-that-improves-the-biosynthesis-of-fluorescent-proteins-in-francisella-tularensis-and-other-gram-negative-bacteria
#20
JOURNAL ARTICLE
Kristen Haggerty, Stuart Cantlay, Emily Young, Mariah K Cashbaugh, Elio F Delatore Iii, Rori Schreiber, Hayden Hess, Daniel R Komlosi, Sarah Butler, Dalton Bolon, Theresa Evangelista, Takoda Hager, Claire Kelly, Katherine Phillips, Jada Voellinger, Robert M Q Shanks, Joseph Horzempa
Utilization of fluorescent proteins is widespread for the study of microbial pathogenesis and host-pathogen interactions. Here, we discovered that linkage of the 36 N-terminal amino acids of FTL_0580 (a hypothetical protein of Francisella tularensis) to fluorescent proteins increases the fluorescence emission of bacteria that express these recombinant fusions. This N-terminal peptide will be referred to as 580N. Western blotting revealed that the linkage of 580N to Emerald Green Fluorescent Protein (EmGFP) in F...
March 14, 2024: Molecular and Cellular Probes
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