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https://www.readbyqxmd.com/read/28748697/portable-lock-in-amplifier-based-electrochemical-method-to-measure-an-array-of-64-sensors-for-point-of-care-applications
#1
Radim Hrdý, Hana Kynclová, Ivana Klepáčová, Martin Bartosik, Pavel Neuzil
We present a portable lock-in amplifier-based electrochemical sensing system. The basic unit (cluster) consists of four electrochemical cells (EC), each containing one pseudo-reference electrode (PRE) and one working electrode (WE). All four ECs are simultaneously interrogated, each at different frequency, with square wave pulses superposed on a sawtooth signal for cyclic voltammetry (CV). Lock-in amplification provides independent read-out of four signals, with excellent noise suppression. We expanded a single cluster system into an array of 16 clusters by using electronic switches...
July 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28747783/engineering-the-geometrical-shape-of-mesenchymal-stromal-cells-through-defined-cyclic-stretch-regimens
#2
Brandan Walters, Tatiana Uynuk-Ool, Miriam Rothdiener, Julian Palm, Melanie L Hart, Jan P Stegemann, Bernd Rolauffs
Stem cells have been predicted to improve disease outcomes and patient lives. Steering stem cell fate - through controlling cell shape - may substantially accelerate progress towards this goal. As mesenchymal stromal cells (MSCs) are continuously exposed in vivo to a dynamically changing biomechanical environment, we hypothesized that exogenous forces can be applied for engineering a variety of significantly different MSC shapes. We applied specific cyclic stretch regimens to human MSCs and quantitatively measured the resulting cell shape, alignment, and expression of smooth muscle (SMC) differentiation markers, as those have been associated with elongated morphology...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28746622/holocene-history-of-a-lake-filling-and-vegetation-dynamics-of-the-serra-sul-dos-caraj%C3%A3-s-southeast-amazonia
#3
José T F Guimarães, Prafulla K Sahoo, Pedro W M Souza-Filho, Mariana M J Costa DE Figueiredo, Luiza S Reis, Marcio S DA Silva, Tarcísio M Rodrigues
Down-core changes in sedimentary facies, elemental geochemistry, pollen, spore, δ13C, δ15N and radiocarbon records from a filled lake, named R4, of the Serra Sul dos Carajás were used to study the relationship between the paleomorphological and paleoecological processes and their significance for Holocene paleoclimatology of the southeast Amazonia. The sediment deposition of the R4 lake started around 9500 cal yr BP. Increase of detrital components from 9500 to 7000 cal yr BP suggests high weathering of surrounding catchment rocks and soils, and deposition into the lake basin under mudflows...
July 24, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28745099/a-new-wireless-enabled-blood-glucose-monitoring-system-that-links-to-a-smart-mobile-device-accuracy-and-user-performance-evaluation
#4
Mark Christiansen, Carmine Greene, Scott Pardo, Mary Ellen Warchal-Windham, Bern Harrison, Robert Morin, Timothy S Bailey
BACKGROUND: These studies investigated the accuracy of the new Contour(®)Next ONE blood glucose monitoring system (BGMS) that is designed to sync with the Contour™ Diabetes app on a smartphone or tablet. METHODS: A laboratory study tested fingertip capillary blood samples from 100 subjects in duplicate using 3 test strip lots, based on ISO 15197:2013 Section 6.3 analytical accuracy standards. A clinical study assessed accuracy per ISO 15197:2013 Section 8 criteria...
May 2017: Journal of Diabetes Science and Technology
https://www.readbyqxmd.com/read/28743668/a-new-haplotype-of-the-coconut-rhinoceros-beetle-oryctes-rhinoceros-has-escaped-biological-control-by-oryctes-rhinoceros-nudivirus-and-is-invading-pacific-islands
#5
Sean D G Marshall, Aubrey Moore, Maclean Vaqalo, Alasdair Noble, Trevor A Jackson
The coconut rhinoceros beetle (CRB; Oryctes rhinoceros) is a major pest of coconut and oil palm, but the discovery and release of Oryctes rhinoceros nudivirus (OrNV) in the 1960s and 70s suppressed the pest such that no new invasions of uninfested islands by CRB were reported for over 30 years after implementation of the biocontrol programme. Surprisingly, a highly damaging outbreak was reported from Guam (2007), which could not be controlled by OrNV. Subsequently, new invasions have been reported from Port Moresby, Papua New Guinea (2009); O'ahu, Hawai'i (2013); and Honiara, Solomon Islands (2015)...
July 22, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28743658/high-level-expression-and-characterization-of-solvent-tolerant-lipase
#6
Yu Wang, Dan Luo, Yunshi Zhao, Shufang Tian, Wenpeng Deng, Chunhua Li, Lixin Ma
In this study, the coding sequence of the lipase from Proteus sp. SW1 was optimized via codon optimization and subjected to expression in Pichia pastoris GS115. The maximum enzyme yield was 387 mg/L in the supernatants of the shake-flask culture. The purified recombinant lipase exhibited a specific activity of 130 U/mg toward p-nitrophenyl Laurate. Its optimum pH and temperature were 8.0 and 40°C, respectively. It was highly stable and even activated in water-miscible solvents, showing over 102% residual activity after 24 h incubation in ethanol, acetone, isopropanol and acetonitrile...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28743302/cognition-culture-and-utility-plant-classification-by-paraguayan-immigrant-farmers-in-misiones-argentina
#7
Monika Kujawska, N David Jiménez-Escobar, Justin M Nolan, Daniel Arias-Mutis
BACKGROUND: This study was conducted in three rural communities of small farmers of Paraguayan origin living in the province of Misiones, Argentina. These Criollos (Mestizos) hail chiefly from departments located in the east of Paraguay, where the climate and flora have similar characteristics as those in Misiones. These ecological features contribute to the continuation and maintenance of knowledge and practices related to the use of plants. METHODS: Fieldwork was conducted between September 2014 and August 2015...
July 25, 2017: Journal of Ethnobiology and Ethnomedicine
https://www.readbyqxmd.com/read/28743015/plant-domestication-wild-date-palms-illuminate-a%C3%A2-crop-s-sticky-origins
#8
Nathan Wales, Benjamin K Blackman
In a new study, previously unknown populations of wild date palm have been identified in remote areas of Oman. Genomic analyses indicate date palm domestication occurred in the eastern portion of the Arabian Peninsula and reveal substantial subsequent gene flow with African palm populations.
July 24, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28741898/development-of-a-new-structural-class-of-broadly-acting-hcv-non-nucleoside-inhibitors-leading-to-the-discovery-of-mk-8876
#9
Casey C McComas, Anandan Palani, Wei Chang, M Katharine Holloway, Charles A Lesburg, Peng Li, Nigel Liverton, Peter T Meinke, David B Olsen, Xuanjia Peng, Richard M Soll, Ajay Ummat, Jie Wu, Jin Wu, Nicolas Zorn, Steven W Ludmerer
Studies directed at developing a broadly acting non-nucleoside inhibitor of HCV NS5B led to the discovery of a novel structural class of 5-aryl benzofurans that simultaneously interact with both the palm I and palm II binding regions. An initial candidate was potent in vitro against HCV GT1a and GT1b replicons, and induced multi-log reductions in HCV viral load when orally dosed to chronic GT1 infected chimpanzees. However, in vitro potency losses against clinically relevant GT1a variants prompted a further effort to develop compounds with sustained potency across a broader array of HCV genotypes and mutants...
July 25, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28741593/electro-cutaneous-stimulation-on-the-palm-elicits-referred-sensations-on-intact-but-not-on-amputated-digits
#10
Marco D'Alonzo, Leonard Frederik Engels, Marco Controzzi, Christian Cipriani
Objective Grasping and manipulation control critically depends on tactile feedback. Without this feedback, the ability for fine control of a prosthesis is limited in upper limb amputees. Early studies have shown that non-invasive electro-cutaneous stimulation (ES) can induce referred sensations that are spread to a wider and/or more distant area, with respect to the electrodes. Building on this, we sought to exploit this effect to provide somatotopically matched sensory feedback to people with partial hand (digital) amputations...
July 25, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28740995/basic-cardiac-life-support-2016-singapore-guidelines
#11
Swee Han Lim, Fong Chi Wee, Tek Siong Chee
Basic Cardiac Life Support (BCLS) or cardiopulmonary resuscitation (CPR) refers to the skills required (without use of equipment) in the resuscitation of cardiac arrest individuals. On recognising cardiac arrest, chest compressions should be initiated. Good quality compressions are with arms extended, elbows locked, shoulders directly over the casualty's chest and heel of the palm on the lower half of the sternum. The rescuer pushes hard and fast, compressing 4-6 cm deep for adults at 100-120 compressions per minute with complete chest recoil...
July 2017: Singapore Medical Journal
https://www.readbyqxmd.com/read/28739162/refining-forelimb-asymmetry-analysis-correlation-with-montoya-staircase-contralateral-function-post-stroke
#12
Moner Ragas, Devipriyanka Nagarajan, Adrian M Corbett
BACKGROUND: Forelimb Asymmetry Test is a simple test of motor function, using exploration behavior of a rat in a novel environment and counting the number of times that a rat touches the wall with either forepaw. Our lab has noticed, however, that there appears to be an increased number of fingertip touches to the wall following a stroke in the impaired forelimb. NEW METHOD: We counted the number of times that the animal either laid its palm flat against the wall of the chamber or touched the wall with only its fingertips, for both the left and right forepaws...
July 21, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28738675/transparent-and-flexible-triboelectric-sensing-array-for-touch-security-applications
#13
Zuqing Yuan, Tao Zhou, Yingying Yin, Ran Cao, Congju Li, Zhong Lin Wang
Tactile sensor with large-scale array and high sensitivity is essential for human-machine interaction, smart wearable devices and mobile network. Here, a transparent and flexible triboelectric sensing array (TSA) with fingertip-sized pixels is demonstrated by integrating ITO electrodes, FEP film and signal-transmission circuits on an undivided palm-sized PET substrate. The sensing pixels can be triggered by the corresponding external contact to induce the electrostatic potential in the transparent electrodes without power consumption, which is individually recognized by the sensor...
July 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28736831/biomimetic-synthesis-of-complex-flavonoids-isolated-from-daemonorops-dragon-s-blood
#14
Dirk Trauner, Matthias Schmid
The dragonbloodins are a pair of complex flavonoid trimers isolated from the palm tree Daemonorops draco, one of the sources of the ancient resin known as "Dragon's Blood". We present a short synthesis that clarifies their relative configuration and sheds light on their origin in Nature. It features biomimetic cascade reactions that involve both ionic and radical intermediates. The biogenetic relationships between dracorhodin, the dracoflavans C, and the dragonbloodins A1 and A2 is discussed.
July 23, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28736147/novel-multifunctional-plant-growth-promoting-bacteria-in-co-compost-of-palm-oil-industry-waste
#15
Clament Fui Seung Chin, Yoshihide Furuya, Mohd Huzairi Mohd Zainudin, Norhayati Ramli, Mohd Ali Hassan, Yukihiro Tashiro, Kenji Sakai
Previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in Malaysia. Little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. We isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: plant growth promoting (fixation of nitrogen; solubilization of phosphorus, potassium, and silicate; production of 3-indoleacetic acid, ammonia, and siderophore), biocontrolling (production of chitinase and anti-Ganoderma activity), and composting (degradation of lignin, xylan, and cellulose)...
July 20, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28736038/comparative-study-of-the-hypocholesterolemic-and-hypolipidemic-activity-of-alginate-and-amidated-alginate-in-rats
#16
Milan Marounek, Zdeněk Volek, Eva Skřivanová, Tomáš Taubner, Akhir Pebriansyah, Dagmar Dušková
Alginate is a copolymer of β-D-mannuronate and α-L-guluronate, which are present in the cell wall of brown algae. The hypocholesterolemic and hypolipidemic activities of alginate and its derivative, which is prepared by a reaction with octadecylamine, were compared in rats fed diets containing cholesterol and palm fat at 10 and 50g/kg, respectively. Amidated alginate at 20g/kg significantly decreased serum cholesterol from 2.93 to 2.00μmol/mL, serum triacylglycerols from 1.66 to 0.92μmol/mL, hepatic cholesterol from 17...
July 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28732258/areca-catechu-palm-tree-nut-chewing-and-seizures-an-observational-study
#17
Farrah J Mateen, Marco Carone, Lhab Tshering, Chencho Dorji, Ugyen Dema, Sara J Grundy, Dili Ram Pokhrel, Damber K Nirola
OBJECTIVE: The objective of this study was to report the impact of chronic abuse of Areca catechu nut wrapped in leaf (also known as doma, quid, paan), the fourth most commonly abused psychoactive substance worldwide, on the frequency of seizures among people with epilepsy. METHODS: People with clinically diagnosed epilepsy (>14years old) residing in the Kingdom of Bhutan were surveyed for self-reported Areca catechu use, demographic variables, epilepsy characteristics, and seizure frequency...
July 18, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28731017/methotrexate-iontophoresis-versus-coal-tar-ointment-in-palmoplantar-psoriasis-a-pilot-study
#18
K Haseena, Sandhya George, Najeeba Riyaz, Sarita Sasidharanpillai, Paul V Puthussery
BACKGROUND: Palmoplantar psoriasis is often disabling and refractory to conventional therapy. Systemic drugs are indicated in its severe form, but side effects are a concern with their use. Methotrexate is one such systemic drug which is effective and cheap. To reduce systemic toxicity, methotrexate has been tried topically but results have been inconsistent due to poor drug penetration into the skin by passive diffusion. Iontophoresis may enhance its absorption and efficacy. AIM: To evaluate the efficacy and safety of topical methotrexate iontophoresis in comparison with coal tar ointment in the treatment of palmoplantar psoriasis...
July 20, 2017: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/28729670/forest-edge-disturbance-increases-rattan-abundance-in-tropical-rain-forest-fragments
#19
Mason J Campbell, Will Edwards, Ainhoa Magrach, Susan G Laurance, Mohammed Alamgir, Gabriel Porolak, William F Laurance
Human-induced forest fragmentation poses one of the largest threats to global diversity yet its impact on rattans (climbing palms) has remained virtually unexplored. Rattan is arguably the world's most valuable non-timber forest product though current levels of harvesting and land-use change place wild populations at risk. To assess rattan response to fragmentation exclusive of harvesting impacts we examined rattan abundance, demography and ecology within the forests of northeastern, Australia. We assessed the community abundance of rattans, and component adult (>3 m) and juvenile (≤3 m) abundance in five intact forests and five fragments (23-58 ha) to determine their response to a range of environmental and ecological parameters...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729463/dietary-pufas-attenuate-nlrp3-inflammasome-activation-via-enhancing-macrophage-autophagy
#20
Lulu Shen, Yan Yang, Tiantong Ou, Chia-Chi C Key, Sarah H Tong, Russel C Sequeira, Jonathan M Nelson, Yan Nie, Zhan Wang, Elena Boudyguina, Swapnil V Shewale, Xuewei Zhu
Dietary PUFAs reduce atherosclerosis and macrophage inflammation, but how NLRP3 inflammasome activation and autophagy influence PUFA-mediated atheroprotection is poorly understood. We fed Ldlr-/- mice diets containing 10% (calories) palm oil (PO) and 0.2% cholesterol, supplemented with an additional 10% of calories as PO, fish oil (FO), echium oil (EO, containing 18:4 n-3), or borage oil (BO, containing 18:3 n-6). Inflammasome activation, autophagic flux, and mitochondrial function were measured in peritoneal macrophages, blood monocytes, or liver from diet-fed mice...
July 20, 2017: Journal of Lipid Research
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