Read by QxMD icon Read


Ognjen Perišić
Physical interactions between proteins are often difficult to decipher. The aim of this paper is to present an algorithm that is designed to recognize binding patches and supporting structural scaffolds of interacting heterodimer proteins using the Gaussian Network Model (GNM). The recognition is based on the (self) adjustable identification of kinetically hot residues and their connection to possible binding scaffolds. The kinetically hot residues are residues with the lowest entropy, i.e., the highest contribution to the weighted sum of the fastest modes per chain extracted via GNM...
March 16, 2018: Pharmaceuticals
Lianbin Xu, Xueyan Lin, Robin R White, Mark D Hanigan, Zhiyong Hu, Qiuling Hou, Yun Wang, Zhonghua Wang
Background: Circulating amino acid (AA) and nitric oxide (NO) concentrations and hepatic gluconeogenesis are affected by previous protein intake. However, information about their relations and islet hormone responses is limited. Objective: This study investigated the associations between islet hormone concentrations with circulating AA and NO concentrations as well as with hepatic gluconeogenesis in lactating rats. Methods: At delivery, 18 Wistar rats aged 14 wk were assigned either to low-protein (LP; 9% protein), standard-protein (SP; 21% protein), or high-protein (HP; 35% protein) diets for 15 d in groups of 6 pups/dam...
March 1, 2018: Journal of Nutrition
Yuan-Jun Xia, Hong Xia, Ling Chen, Qing-Shui Ying, Xiang Yu, Li-Hua Li, Jian-Hua Wang, Ying Zhang
Bone morphogenetic protein-2 (BMP-2) serves an important role in the development of bone and cartilage. However, administration of BMP-2 protein alone by intravenous delivery is not very effective. Sustained delivery of stabilized BMP-2 by carriers has been proven necessary to improve the osteogenesis effect of BMP-2. The present study constructed a novel drug delivery system using dextran sulfate (DS)-chitosan (CS) microspheres and investigated the efficiency of the delivery system on recombinant human bone morphogenetic protein (rhBMP-2)...
April 2018: Experimental and Therapeutic Medicine
Zixing Dong, Xiaoling Chen, Ke Cai, Zhixin Chen, Hongbin Wang, Peng Jin, Xiaoguang Liu, Kugenthiren Permaul, Suren Singh, Zhengxiang Wang
Due to its high protein secretion capacity, simple nutritional requirements and the GRAS (generally regarded as safe) status, Bacillus licheniformis is widely used as a host for the industrial production of enzymes, antibiotics and peptides. However, as compared with its close relative B. subtilis , little is known about physiology and stress responses of B. licheniformis . To explore its temperature-stress metabolome, strains B. licheniformis ATCC 14580 and B186, with respective optimal growth temperatures of 42°C and 50°C, were cultured at 42°C, 50°C and 60°C and their corresponding metabolic profiles determined by gas chromatography/mass spectrometry (GC/MS) and multivariate statistical analyses...
March 15, 2018: Journal of Microbiology and Biotechnology
Adam H Lewis, Ankur Chugh, Sarah A Sobotka
A 7-year-old girl with 20q13.33 deletion and a history of generalized convulsive epilepsy presented to the Developmental and Behavioral Pediatrics Clinic due to concerns about her behavioral outbursts in the context of overall delayed development. Evaluation by the Developmental and Behavioral and Gastroenterology teams revealed failure to thrive (FTT) as the primary cause of the behavioral outbursts and developed a high-calorie, high-fat, high-protein nutritional counseling plan. Children who have FTT and a genetic disorder are often thought to not thrive because of their underlying genetic disorder; however, feeding skills and nutritional intake need to be thoroughly investigated before determining an etiology for FTT...
March 1, 2018: Pediatric Annals
Justine M Abais-Battad, Hayley Lund, Daniel J Fehrenbach, John Henry Dasinger, David L Mattson
The current study, performed in Dahl Salt-Sensitive (SS) and SS-Rag1-/- rats lacking T- and B- lymphocytes, tested the hypothesis that immune cells amplify salt-sensitive hypertension and kidney damage in response to a high protein diet. After weaning, SS and SS-Rag1-/- rats were placed on an isocaloric, 0.4% NaCl diet containing normal (18%) or high (30%) protein. At 9 weeks of age, rats were switched to a 4.0% NaCl diet containing the same amount of dietary protein and maintained on the high salt diet for 3 weeks...
March 14, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Stacy Pelekhaty, Jay Menaker
Prader-Willi Syndrome (PWS) is a genetic condition that results in a constellation of symptoms and typically results in hyperphagia and obesity in adulthood. Critically ill adults with PWS present a unique challenge to the nutrition professional, particularly when they require support modalities such as extracorporeal membrane oxygenation (ECMO). The purpose of this case study is to review the nutrition care of a critically ill adult patient with PWS who required venovenous ECMO. The patient was successfully managed with a hypocaloric, high-protein approach, which did not result in the diagnosis of malnutrition during his hospitalization...
March 12, 2018: Nutrition in Clinical Practice
Charlotte M Buckley, Bobby G Stuijfzand, Peter J Rogers
Selecting savoury foods after consuming a protein depleted diet has been suggested to reflect protein seeking behaviour. The modern diet contains a large number of processed foods, many of which are highly savoury to taste, but not necessarily high in protein. The present two studies aimed to investigate the relationship between savoury taste and protein content (actual and participant estimated). Participants (S1 n = 20, S2 n = 37) completed 100 mm VAS ratings of sensory and nutritional qualities of 18 familiar foods, categorised as sweet low protein, savoury low protein and savoury high protein...
March 9, 2018: Physiology & Behavior
G A Mejicanos, J W Kim, C M Nyachoti
Tail-end dehulling of canola meal (CM) has been shown to reduce dietary fiber and increase crude protein content in the dehulled meal. The application of this procedure also increased the total and non-phytate P content in the dehulled meal. However, it is unclear if dehulling affects P digestibility in the different fractions (i.e., the dehulled meal and the coarse fraction) and if it differs when fed to growing pigs at 2 different BW. Therefore, 2 experiments were conducted to determine the apparent (ATTD) and standardized (STTD) total tract digestibility of P in dehulled CM fed to growing pigs...
February 24, 2018: Journal of Animal Science
Vikram Singh Gaur, Lallan Kumar, Supriya Gupta, J P Jaiswal, Dinesh Pandey, Anil Kumar
In this study, we report the isolation and characterization of the mRNA encoding OPAQUE2 (O2) like TF of finger millet (FM) ( Eleusine coracana) ( EcO2 ). Full-length EcO2 mRNA was isolated using conserved primers designed by aligning O2 mRNAs of different cereals followed by 3' and 5' RACE (Rapid Amplification of cDNA Ends). The assembled full-length EcO2 mRNA was found to contain an ORF of 1248-nt coding the 416 amino acids O2 protein. Domain analysis revealed the presence of the BLZ and bZIP-C domains which is a characteristic feature of O2 proteins...
March 2018: 3 Biotech
Imtiaz Hussain Raja Abbasi, Farzana Abbasi, Mohamed E Abd El-Hack, Ayman A Swelum, Junhu Yao, Yangchun Cao
Microbial degradation in the rumen and dietary availability of methionine amino acid have been reported as limiting in dairy ruminants. The aim of the present study was to examine the post-ruminal effects of feeding ruminants different concentrations of rumen-protected methionine (RPM) in low crude protein diets using the long-term rumen simulation method (Rusitec) followed by in vitro abomasum and ileum digestibility technique. The experiment contained four treatment groups: (1) high protein, without RPM supplementation (HP); (2) low protein, without RPM supplementation (LP); (3) low protein supplementation with low RPM (LPLM); and (4) low protein supplementation with high RPM (LPHM) mixed per 20 ± 0...
March 9, 2018: AMB Express
Pauline Uí Dhuibhir, Noelle Collura, Declan Walsh
Oral nutritional supplements (ONS) are frequently prescribed for those at risk of malnutrition. Palatability is an important factor in long-term compliance. ONS selection is typically dietitian led, but the degree to which individual perceptions of palatability influence dietitian clinical decision making is unclear. This study aimed to explore factors that influence dietitians' ONS clinical practice, evaluate dietitian hedonic preferences and overall impression of specific ONS products, and study phenylthiocarbamide (PTC) sensitivity in relation to ONS hedonic ratings...
March 9, 2018: Journal of Dietary Supplements
Roberto Ferro, Maja Rennig, Cristina Hernández-Rollán, Daniel O Daley, Morten H H Nørholm
BACKGROUND: The market for recombinant proteins is on the rise, and Gram-positive strains are widely exploited for this purpose. Bacillus subtilis is a profitable host for protein production thanks to its ability to secrete large amounts of proteins, and Lactococcus lactis is an attractive production organism with a long history in food fermentation. RESULTS: We have developed a synbio approach for increasing gene expression in two Gram-positive bacteria. First of all, the gene of interest was coupled to an antibiotic resistance gene to create a growth-based selection system...
March 8, 2018: Microbial Cell Factories
Connie W Bales, Kathryn N Porter Starr, Melissa C Orenduff, Shelley R McDonald, Karen Molnar, Aubrey K Jarman, Ann Onyenwoke, Hillary Mulder, Martha E Payne, Carl F Pieper
Background: Women have higher rates of obesity than men and develop more pronounced functional deficits as a result. Yet, little is known about how obesity reduction affects their functional status, including whether their responses differ when protein intake is enhanced. Objective: The aim of this study was to confirm the feasibility of delivery of a higher-protein (balanced at each meal) calorie-restricted diet in obese women and determine its efficacy for influencing function and retention of lean mass...
May 1, 2017: Curr Dev Nutr
Sandeep S Dhawan, Shuang Xia, David S Tait, Christoffer Bundgaard, Ellen Bowman, Verity J Brown
RATIONALE: Delivering orally bioavailable drugs to rodents is an important component to investigating that route of administration in novel treatments for humans. However, the traditional method of oral gavage requires training, is stressful, and can induce oesophageal damage in rodents. OBJECTIVES: To demonstrate a novel administrative technique-palatable gelatine tablets-as a stress-free route of oral delivery. METHODS: Twenty-four male Lister hooded rats were sacrificed for brain tissue analysis at varying time-points after jelly administration of 30 mg/kg of the wake-promoting drug modafinil...
March 6, 2018: Psychopharmacology
Guoqing Wang, Mark A Cline, Elizabeth R Gilbert
The goal of this research was to determine the effect of dietary macronutrient composition on peripheral neuropeptide Y (NPY)-induced changes in adipose tissue dynamics in chicks. Chicks were fed one of three isocaloric diets from the day of hatch: high carbohydrate (HC), high fat (HF), or high protein (HP). On day 4 post-hatch, 0 (vehicle), 60, or 120 µg/kg BW of NPY was injected intraperitoneally, and subcutaneous, clavicular and abdominal adipose tissue samples were collected at 1 and 3 h post-injection...
March 3, 2018: General and Comparative Endocrinology
György Szabó, György István Túrós, Sándor Kolok, Mónika Vastag, Zsuzsanna Sánta, Miklós Dékány, György I Lévay, István Greiner, Minami Natsumi, Watanabe Tatsuya, Gyorgy M Keseru
Metabotropic glutamate receptor 2 (mGluR2) positive allosteric modulators (PAMs) have been implicated as potential pharmacotherapy for psychiatric conditions. Screening our corporate compound deck, we identified a benzotriazole fragment (4) that was rapidly optimized to a potent and metabolically stable early lead (16). The highly lipophilic character of 16, together with its limited solubility, permeability and high protein binding, however, did not allow to reach the proof of concept in vivo. Since further attempts on the optimization of druglike properties were unsuccessful, the original hit 4 has been revisited and was optimized following the principles of fragment based drug discovery (FBDD)...
March 5, 2018: Journal of Medicinal Chemistry
Mitchell S Cappell, Ahmed Edhi, Mitual Amin
RATIONALE: Primary intestinal lymphangiectasia (PIL) is a rare, presumably congenital lesion that is usually diagnosed in patients < 3 years old, is rarely first diagnosed in adulthood, and when first diagnosed in adulthood typically presents with symptoms for many years. Although PIL is often identified by endoscopic abnormalities, it must be emphasized that the jejunoileum/distal duodenum must be intubated for diagnosis because the lesions are present in these regions. This work demonstrates that 1)-PIL can occur in an octogenarian; 2)-shows that the characteristic endoscopic findings are not found at colonoscopy without terminal ileal intubation; and 3)-may be missed at standard EGD without distal duodenal intubation...
January 2018: Medicine (Baltimore)
Evangelia I Iatrou, Elianta Kora, Athanasios S Stasinakis
The use of duckweed-based wastewater treatment systems for producing biomass with high crude protein and starch content was investigated in the current study. For this reason, three lab-scale systems were used; System 1 was planted with Lemna minor, System 2 with Lemna gibba and System 3 with the combination of the two duckweeds. The studied duckweeds were cultivated using secondary treated wastewater as substrate (Phase A), in presence of excess NH4 -N (Phase B) and using water with no nutrients (Phase C)...
March 5, 2018: Environmental Technology
Brandon Carter, Hasmukh Patel, David M Barbano, MaryAnne Drake
Traditionally most protein ingredients are sold as a powder due to transport ease and longer shelf life. Many high-protein powder ingredients such as milk protein concentrate with 85% protein and micellar casein concentrate have poor rehydration properties (e.g., solubility) after storage, which might limit their use. An alternative to the production of dried protein ingredients is the option to use liquid protein ingredients, which saves the cost of spray drying, but may also improve flavor and offer different functional properties...
February 28, 2018: Journal of Dairy Science
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"