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https://www.readbyqxmd.com/read/27925685/in-vitro-characterization-of-synthoplate-%C3%A2-synthetic-platelet-technology-and-its-in-vivo-evaluation-in-severely-thrombocytopenic-mice
#1
Meenal Shukla, Ujjal D S Sekhon, Venkaiah Betapudi, Wei Li, DaShawn A Hickman, Christa L Pawlowski, Mitchell R Dyer, Matthew D Neal, Keith R McCrae, Anirban Sen Gupta
BACKGROUND: Platelet transfusion applications face severe challenges due to the limited availability and portability, high risk of contamination and short shelf-life of platelets. Therefore there is significant interest in synthetic platelet substitutes that can render hemostasis while avoiding these issues. Platelets promote hemostasis by injury site-selective adhesion and aggregation, and propagation of coagulation reactions on their membrane. Based on these mechanisms, we have developed a synthetic platelet technology (SynthoPlate(™) ) that integrates platelet-mimetic site-selective 'adhesion' and 'aggregation' functionalities via heteromultivalent surface-decoration of lipid vesicles with Von Willebrand Factor-binding, collagen-binding and active platelet integrin GPIIb-IIIa-binding peptides...
December 7, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27913744/effects-of-atorvastatin-on-adp-arachidonic-acid-collagen-and-epinephrine-induced-platelet-aggregation
#2
Lei Zhao, Demin Liu, Bin Liu, Haijuan Hu, Wei Cui
OBJECTIVE: Atorvastatin reduces the incidence of cardiovascular events. However, the effects of atorvastatin on platelet aggregation are unknown. METHODS: Blood samples were obtained from 126 healthy volunteers. Prepared isolated platelet suspensions were adjusted with saline to three different concentrations of 100 × 10(9), 300 × 10(9), and 600 × 10(9) platelets/L. Platelet samples were incubated with atorvastatin (10(-7 )mol/L, 10(-6 )mol/L or 10(-5 )mol/L), and stimulated with ADP (10 µmol/L), arachidonic acid (0...
December 2, 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/27892864/influence-of-a-low-dosage-of-clopidogrel-on-platelet-function-in-cats-as-measured-by-the-platelet-function-analyser-pfa-100-and-the-multiplate-analyser
#3
M Teuber, R Mischke
The antiplatelet drug clopidogrel is widely used for prophylaxis of arterial thromboses in cats in a standard dosage of 18.75mg per cat once daily. The aim of the study was to verify if a reduced daily dose of 10mg clopidogrel per cat has a similar antiplatelet effect as the standard dosage. Platelet function was measured with the platelet function analyser PFA-100(®) and a novel impedance aggregometer. Suitability of the platelet function analyser was tested in citrated blood samples of 59 healthy cats and reference ranges were established...
September 17, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27872729/mild-bleeders-diagnosis-is-elusive-in-large-number-of-patients
#4
Mrinalini Kotru, Deepti Mutereja, Abhishek Purohit, Seema Tyagi, Manoranjan Mahapatra, Renu Saxena, Hara Prasad Pati
BACKGROUND: Bleeding is a common clinical presentation. Even patients with mild bleeding disorders are extensively investigated for ascertaining the cause. The present study was conducted in order to evaluate the extent of the possibility of diagnosis in mild bleeding disorders. MATERIAL AND METHODS: This was a prospective study of patients referred for work up of mild bleeding for a period of 13 months. A complete blood count, peripheral smear examination, Prothrombin time, Partial Thromboplastin time and Thrombin Time, Platelet Aggregometry test, tests for von Willebrand's disease and Platelet Factor 3 availability were measured...
2016: Mediterranean Journal of Hematology and Infectious Diseases
https://www.readbyqxmd.com/read/27864862/heparin-induced-multi-electrode-aggregometry-method-for-heparin-induced-thrombocytopenia-testing-communication-from-the-ssc-of-the-isth
#5
M-C Morel-Kopp, F Mullier, V Gkalea, T Bakchoul, V Minet, I Elalamy, C M Ward
No abstract text is available yet for this article.
September 26, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27848264/underlying-mechanism-and-specific-prevention-of-hemolysis-induced-platelet-activation
#6
Thomas Gremmel, Simone Fedrizzi, Günter Weigel, Beate Eichelberger, Simon Panzer
Thromboembolic complications significantly impair the outcome of hemolytic disorders. We hypothesized that red cell adenosine diphosphate (ADP) release results in significant platelet activation in hemolysis and that this prothrombotic state can be prevented by inhibition of the ADP P2Y12 receptor. In the current study, we therefore sought to investigate the mechanism and inhibition of hemolysis-induced platelet activation. The expression of activated integrin αIIbß3 was determined by flow cytometry, and platelet aggregation was assessed by multiple electrode platelet aggregometry...
November 16, 2016: Platelets
https://www.readbyqxmd.com/read/27797407/platelet-aggregometry-cannot-identify-uremic-platelet-dysfunction-in-heart-failure-patients-prior-to-cardiac-surgery
#7
Josh Showalter, Nghia D Nguyen, Samer Baba, Chi Hyun Lee, Jing Ning, Kimberly Klein, M Amer Wahed, Ashok Tholpady
BACKGROUND: Patients with heart failure often have concomitant renal disease which can result in uremic platelet dysfunction. Determining whether uremia has affected platelets by platelet aggregometry can be challenging in these patients since they are often on antiplatelet medications. This study was undertaken to determine if platelet aggregation studies could identify heart failure patients at risk for uremic bleeding prior to cardiac surgery. METHODS: Platelet aggregation studies from three groups were studied and compared: 17 heart failure patients with mild to moderate renal impairment, 17 heart failure patients without renal abnormalities and 17 healthy volunteers...
October 31, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27789259/antiplatelet-effect-of-differentially-charged-pegylated-lipid-polymer-nanoparticles
#8
Eduardo Fuentes, Basit Yameen, Soung-Jae Bong, Carolina Salvador-Morales, Ivan Palomo, Cristian Vilos
PEGylated nanoparticles have been extensively investigated in different platforms for drug delivery. However, the physiological effects related to platelet activation, and the potential procoagulant activity which could lead to thrombosis and further cardiovascular diseases have not been widely examined. In this work, we studied the effect of differentially charged PEGylated lipid-polymer nanoparticles in the human platelet aggregation and activation by light transmission aggregometry and flow cytometry. PEGylated nanoparticles inhibited the platelet aggregation with a dose dependency (350, 700, and 1400μg/mL) in both ADP- and collagen-induced platelet aggregation, and P-selectin expression...
October 24, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27777753/coagulative-safety-of-epidural-catheters-after-major-upper-gastrointestinal-surgery-advanced-and-routine-coagulation-analysis-in-38-patients
#9
Owain Thomas, Hampus Rein, Karin Strandberg, Ulf Schött
BACKGROUND: The risk of spinal haematoma in patients receiving epidural catheters is estimated using routine coagulation tests, but guidelines are inconsistent in their recommendations on what to do when results indicate slight hypocoagulation. Postoperative patients are prone to thrombosis, and thromboelastometry has previously shown hypercoagulation in this setting. We aimed to better understand perioperative haemostasis by comparing results from routine and advanced tests, hypothesizing that patients undergoing major upper gastrointestinal surgery would be deficient in vitamin K-dependent coagulation factors because of malnutrition, or hypocoagulative because of accumulation of low molecular weight heparin (LMWH)...
2016: Perioperative Medicine
https://www.readbyqxmd.com/read/27768934/the-proteasome-regulates-collagen-induced-platelet-aggregation-via-nuclear-factor-kappa-b-nf%C3%A4-b-activation
#10
Katharina Grundler, Raffaela Rotter, Sloane Tilley, Joachim Pircher, Thomas Czermak, Mustaf Yakac, Erik Gaitzsch, Steffen Massberg, Florian Krötz, Hae-Young Sohn, Ulrich Pohl, Hanna Mannell, Bjoern F Kraemer
INTRODUCTION: Platelets possess critical hemostatic functions in the system of thrombosis and hemostasis, which can be affected by a multitude of external factors. Previous research has shown that platelets have the capacity to synthesize proteins de novo and more recently a multicatalytic protein complex, the proteasome, has been discovered in platelets. Due to its vital function for cellular integrity, the proteasome has become a therapeutic target for anti-proliferative drug therapies in cancer...
October 13, 2016: Thrombosis Research
https://www.readbyqxmd.com/read/27761577/monitoring-of-coagulation-factor-therapy-in-patients-with-von-willebrand-disease-type-3-using-a-microchip-flow-chamber-system
#11
Anna Ågren, Margareta Holmström, David E Schmidt, Kazuya Hosokawa, Margareta Blombäck, Paul Hjemdahl
Patients with type 3 von Willebrand disease (VWD-3) have no measurable levels of VW factor (VWF) and usually require treatment with VWF-FVIII concentrate to prevent and/or stop bleeding. Even though the patients are treated prophylactically, they may experience bleeding symptoms. The aim of this study was to evaluate the effect of VWF-FVIII concentrate treatment in VWD-3 patients with the Total Thrombus Analysis System (T-TAS(®)), which measures thrombus formation under flow conditions. Coagulation profiles of 10 VWD-3 patients were analysed using T-TAS before and 30 minutes after VWF-FVIII concentrate (Haemate(®)) injection...
October 20, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27761425/comparison-of-antiplatelet-activity-of-garlic-tablets-with-cardio-protective-dose-of-aspirin-in-healthy-volunteers-a-randomized-clinical-trial
#12
Mojtaba Shafiekhani, Pouya Faridi, Javad Kojuri, Soha Namazi
OBJECTIVE: Some of the adverse effects of aspirin including peptic ulcers, gastrointestinal bleeding and aspirin resistance compelled researchers to find a suitable alternative with fewer adverse effects. In this clinical trial, we aimed to find the effective antiplatelet dose of garlic. MATERIALS AND METHODS: This randomized controlled clinical trial (RCT) was conducted on 62 healthy volunteers of 20-50 years old. All volunteers used 80 mg aspirin per day for 1 week and at the end of this time, platelet aggregation (PA) induced by 4 agonists acting in aggregation pathway including adenosinediphosphate (20 μmol/l), epinephrine (20 μmol/l), collagen(0...
September 2016: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/27742347/acetylsalicylic-acid-resistance-after-simultaneous-pancreas-kidney-transplantation
#13
D Praksch, B Sandor, K K Nagy, M Viola, A Toth, K Toth, P Szakaly, A Varga
BACKGROUND: The most common conditions leading to death after simultaneous pancreas-kidney transplantations (SPKs) are cardiovascular diseases. The aim of this study was to test the platelet aggregation inhibitor acetylsalicylic acid (ASA) resistance in patients after SPKs, including investigations into the triggering factors. METHODS: Thirty-two patients (22 men, 10 women; overall age, 47.4 ± 8.6 years) were involved in our study and took 100 mg ASA per day. We used optical platelet aggregometry to detect resistance...
September 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27736703/platelet-function-in-baboons-and-humans-a-comparative-study-of-whole-blood-using-impedance-platelet-aggregometry-multiplate%C3%A2
#14
Martin Ponschab, Martijn van Griensven, Stefan Heitmeier, Volker Laux, Christoph J Schlimp, Andreas Calatzis, Soheyl Bahrami, Heinz Redl, Herbert Schöchl
BACKGROUND: Platelets play a pivotal role in coagulation, inflammation and wound healing. Suitable animal models that have the potential to mimic human platelet function are limited. The objective of the current study was to compare platelet aggregation response in the whole blood of baboons and humans using impedance aggregometry. METHODS: Blood was drawn from 24 anesthetised male baboons and 25 healthy volunteers. The platelet aggregation response was determined by impedance aggregometry (Multiplate®)...
October 6, 2016: Thrombosis Research
https://www.readbyqxmd.com/read/27735053/%C3%AE-blockers-are-associated-with-decreased-leucocyte-platelet-aggregate-formation-and-lower-residual-platelet-reactivity-to-adenosine-diphosphate-after-angioplasty-and-stenting
#15
Silvia Lee, Markus Durstberger, Beate Eichelberger, Christoph W Kopp, Renate Koppensteiner, Simon Panzer, Thomas Gremmel
BACKGROUND: The beneficial effects of β-blockers on the long-term prognosis of patients with cardiovascular disease may in part be attributable to decreased platelet activation. In this prospective cohort study, we sought to investigate the impact of concomitant β-blocker therapy on sensitive markers of platelet activation and aggregation. MATERIALS AND METHODS: Monocyte-platelet (MPA) and neutrophil-platelet aggregate (NPA) formation in vivo and in response to the platelet agonist adenosine diphosphate (ADP) were determined by flow cytometry in 258 patients undergoing angioplasty and stenting...
December 2016: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27731498/effects-of-firocoxib-flunixin-meglumine-and-phenylbutazone-on-platelet-function-and-thromboxane-synthesis-in-healthy-horses
#16
Brenna N Burkett, John M Thomason, Holly M Hurdle, Robert W Wills, Robin L Fontenot
OBJECTIVE: Determine the effects of nonsteroidal anti-inflammatory drugs (NSAID) on platelet function and thromboxane synthesis immediately after drug administration and following 5 days of NSAID administration in healthy horses. STUDY DESIGN: Randomized cross-over study. ANIMALS: Healthy adult horses (n=9; 6 geldings and 3 mares). METHODS: Horses received either flunixin meglumine (1.1 mg/kg IV every 12 hours), phenylbutazone (2...
October 12, 2016: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/27724023/coagulopathy-in-patients-with-acute-pulmonary-embolism-a-pilot-study-of-whole-blood-coagulation-and-markers-of-endothelial-damage
#17
Per Lehnert, Pär I Johansson, Sisse R Ostrowski, Christian H Møller, Lia E Bang, Peter Skov Olsen, Jørn Carlsen
Whole blood coagulation and markers of endothelial damage were studied in patients with acute pulmonary embolism (PE), and evaluated in relation to PE severity. Twenty-five patients were enrolled prospectively each having viscoelastical analysis of whole blood done using thrombelastography (TEG) and Multiplate aggregometry. Fourteen of these patients were investigated for endothelial damage by ELISA measurements of Syndecan-1 (endothelial glycocalyx degradation), soluble endothelial Selectin (endothelial cell activation), soluble Thrombomodulin (endothelial cell injury) and Histone Complexed DNA fragments (endothelial cytotoxic histones)...
October 11, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27721320/high-on-treatment-platelet-reactivity-risk-factors-and-5-year-outcomes-in-patients-with-acute-myocardial-infarction
#18
Martin Jakl, Robert Sevcik, Ilona Fatorova, Jan M Horacek, Radek Pudil
OBJECTIVE: The aim of the present study was to assess long-term prognostic value of high on-treatment platelet reactivity (HTPR) in patients after acute myocardial infarction (MI) and its association with possible risk factors. METHODS: This prospective, case-control study was an observation of 198 patients who had acute MI. Response to aspirin and clopidogrel was assessed using impedance aggregometry. Patients were divided into groups of adequate response, dual poor responsiveness (DPR), poor responsiveness to aspirin (PRA), and poor responsiveness to clopidogrel (PRC)...
October 5, 2016: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/27718888/platelet-rich-plasma-for-tissue-regeneration-can-be-stored-at-room-temperature-for-at-least-five-days
#19
Gary W Moore, James C Maloney, Robert A Archer, Kerri L Brown, Katarzyna Mayger, Elaine S Bromidge, Mitra F Najafi
BACKGROUND: Autologous platelet-rich plasma (PRP) is gaining increasing use as a wound healing promoter in a variety of clinical settings, including dentistry. Fresh PRP is often used, necessitating daily draws. The present study investigates the possibility of using stored PRP without having to freeze it by storing PRP under variable conditions and assessing growth factor release as a surrogate marker of continued viability. METHODS: Freshly drawn PRP was stored in oxygen permeable and non-oxygen permeable containers under conditions of constant agitation with or without added prostaglandin, intermittent agitation and no agitation, over an 8-day period...
October 8, 2016: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/27716685/platelet-aggregometry-interpretation-using-rotem-part-ii
#20
Vandana Bhardwaj, Poonam Malhotra Kapoor
No abstract text is available yet for this article.
October 2016: Annals of Cardiac Anaesthesia
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