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https://www.readbyqxmd.com/read/29775206/prospective-determination-of-aspirin-sensitivity-in-patients-resistant-to-low-dose-aspirin-a-proof-of-concept-study
#1
Erica S Westphal, Michelle Rainka, Michelle Amsler, Traci Aladeen, Caitlin Wisniewski, Vernice Bates, Fran M Gengo
This study tested the capability of an assay to predict aspirin response and reduce ischemic events, and healthcare costs, and delays to optimal treatment. Patients who needed aspirin in the course of normal medical care were included. Patients were excluded if they had disorders affecting platelet function, alcohol use within 24 hours of a test, or NSAID use. Dose escalation of chewable aspirin from 81 mg, to 162 mg, to 325 mg daily occurred based on the results of whole blood impedance aggregation testing to the agonists, collagen (1ug/mL, 5 ug/mL) and arachidonate (0...
May 18, 2018: Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29775202/the-development-of-an-in-vitro-assay-for-the-prospective-determination-of-aspirin-sensitivity
#2
Erica S Westphal, Caitlin Wisniewski, Michelle Rainka, Nicholas M Smith, Vernice Bates, Fran M Gengo
Aspirin remains the standard for stroke prophylaxis. However, as many as 20%-25% of patients may fail to show a full response to aspirin. Ideally, patients who are resistant to aspirin could be identified, then receive an increased dose of aspirin or be changed to an alternative therapy more efficiently. We have developed an in vitro assay that may make this possible. Healthy volunteers (n = 13) between 18 and 50 years of age were tested for both ex vivo and in vivo responses to aspirin. Dimethyl sulfoxide (DMSO) was selected as the solvent for aspirin in the assay...
May 18, 2018: Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29759064/propolis-ethanolic-extracts-reduce-adenosine-diphosphate-induced-platelet-aggregation-determined-on-whole-blood
#3
Mirza Bojić, Andrea Antolić, Maja Tomičić, Željko Debeljak, Željan Maleš
BACKGROUND: Propolis is a well-known bee product containing more than 2000 identified compounds. It has many beneficial effects on human health that include antibacterial, antiviral, anticancer and hepatoprotective justifying its use as a dietary supplement. Platelet aggregation plays crucial role in thrombus formation that can cause stroke or heart attacks. As cardiovascular diseases, including those caused by thrombus formation, are related to 50% of deaths of Western population, the objective of this study was to determine antiaggregatory activity of propolis on platelet aggregation on the whole blood samples...
May 14, 2018: Nutrition Journal
https://www.readbyqxmd.com/read/29754149/the-role-of-dipyrone-in-the-high-development-of-on-treatment-platelet-reactivity-in-acetylsalicylic-acid-treated-patients-undergoing-peripheral-artery-revascularization
#4
Jan Hartinger, Robert Novotny, Jana Bilkova, Tomas Kvasnicka, Petr Mitas, Martin Sima, Jaroslav Hlubocky, Jan Kvasnicka, Ondrej Slanar, Jaroslav Lindner
OBJECTIVE: To evaluate the effects of dipyrone on sensitivity to Aspirin [acetyl salicylic acid, (ASA)] in patients who underwent peripheral artery vascular reconstruction. SUBJECTS AND METHODS: Impedance aggregometry and light transmission aggregometry were used to determine the effects of dipyrone on ASA treatment in 21 patients. Blood samples were drawn in a 7-day period after the surgery. The cut-off value for high on-treatment platelet reactivity (HTPR) was set at < 65% of aggregation inhibition for impedance aggregometry...
May 13, 2018: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/29749651/effects-of-immunosuppressive-agents-on-the-hemostatic-system-in-normal-dogs
#5
John M Thomason, Todd M Archer, Robert W Wills, Andrew J Mackin
BACKGROUND: In dogs, the effects of immunosuppressive medications on hemostasis are not well known. HYPOTHESIS/OBJECTIVES: The objective was to determine the effects of immunosuppressive medications on primary and secondary hemostasis. Our hypothesis was that cyclosporine and prednisone would increase markers of hypercoagulability and thromboxane synthesis, while azathioprine, mycophenolate mofetil, and leflunomide would have minimal effects on hemostasis. ANIMALS: Eight healthy dogs...
May 10, 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29747861/effects-of-carvedilol-versus-metoprolol-on-platelet-aggregation-in-patients-with-acute-coronary-syndrome-the-plate-block-study
#6
Federica Ilardi, Giuseppe Gargiulo, Gabriele Giacomo Schiattarella, Giuseppe Giugliano, Roberta Paolillo, Giovanni Menafra, Elena De Angelis, Laura Scudiero, Anna Franzone, Eugenio Stabile, Cinzia Perrino, Plinio Cirillo, Carmine Morisco, Raffaele Izzo, Valentina Trimarco, Giovanni Esposito
Platelet aggregation plays a pivotal role in acute coronary syndrome (ACS). In this setting, β-blockers (BBs) are used to counteract the effects of catecholamines on heart. Circulating catecholamines can also potentiate platelet reactivity, mainly through α2 - and β2 -adrenoceptors on human platelets' surface, thus BB may affect platelet aggregation; however, the effects of different BBs on platelet aggregation in contemporary-treated patients with ACS have been poorly investigated. One hundred patients with ACS on dual antiplatelet therapy with aspirin and ticagrelor were randomized to receive treatment with carvedilol, a nonselective BB (n = 50), or metoprolol, a selective β1 -blocker (n = 50), at maximum tolerated dose...
April 3, 2018: American Journal of Cardiology
https://www.readbyqxmd.com/read/29741257/laboratory-diagnosis-of-clinically-relevant-platelet-function-disorders
#7
REVIEW
P Gresele, E Falcinelli, L Bury
Inherited platelet function disorders (IPFDs) represent a significant fraction of congenital hemorrhagic disorders, and may be associated with bleeding of considerable severity. IPFDs may be difficult to diagnose and a preliminary accurate clinical examination and an objective evaluation of the severity of the bleeding history are mandatory. The laboratory investigation of IPFDs should follow a rational algorithm based on a streamlined panel of laboratory tests with subsequent steps of increasing levels of complexity...
May 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29737900/effects-of-age-gender-and-menstrual-cycle-on-platelet-function-assessed-by-impedance-aggregometry
#8
G Berlin, M Hammar, L Tapper, N Tynngård
Platelets are needed to prevent or arrest bleeding and aggregate at the site of injury upon vascular damage. Platelets express receptors for estrogens which might affect the function of the platelets and their hemostatic ability. The aim was to identify possible differences in platelet function related to age, gender, and phases of the menstrual cycle by use of impedance aggregometry with Multiplate. In the first part of the study, platelet function was assessed in 60 healthy individuals (30 men and 30 women) in each of three age groups (20-25, 40-45, and 60-65 years)...
May 8, 2018: Platelets
https://www.readbyqxmd.com/read/29737075/-adjusting-platelet-counts-for-platelet-aggregation-tests
#9
Li-Qin Ling, Xin-Chun Yang, Hao Chen, Chao-Nan Liu, Si Chen, Hong Jiang, Ya-Xiong Jin, Jing Zhou
OBJECTIVE: To explore a better method to adjust platelet counts for light transmission aggregometry (LTA). METHODS: Blood samples from 36 healthy participants aged from 18 to 50 yr. were collected.Platelet-rich plasma (PRP) was diluted using platelet-poor plasma (PPP) and physiological saline (PS),respectively,in a ratio of 1.5,2,2.5 and 3 times. Platelet aggregation was induced by adenosine diphosphate (ADP),arachidonic acid (ARA),collagen (COL), epinephrine (EPI),or ristocetin (RIS)...
March 2018: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/29723157/testosterone-and-dihydrotestosterone-reduce-platelet-activation-and-reactivity-in-older-men-and-women
#10
Kamil Karolczak, Lucyna Konieczna, Tomasz Kostka, Piotr J Witas, Bartlomiej Soltysik, Tomasz Baczek, Cezary Watala
The cardiovascular effects of testosterone and dihydrotestosterone are generally attributed to their modulatory action on lipid and glucose metabolism. However, no ex vivo studies suggest that circulating androgen levels influence the activation and reactivity of blood platelets - one of the main components of the haemostasis system directly involved in atherosclerosis. The levels of testosterone, dihydrotestosterone and oestradiol in plasma from men and women aged from 60 to 65 years were measured by LC-MS; the aim was to identify any potential relationships between sex steroid levels and the markers of platelet activation (surface membrane expression of GPII/IIIa complex and P-selectin) and platelet reactivity in response to arachidonate, collagen or ADP, monitored with whole blood aggregometry and flow cytometry...
May 2, 2018: Aging
https://www.readbyqxmd.com/read/29719963/point-of-care-tests-verifynow-p2y12-and-innovance-pfa-p2y-compared-to-light-transmittance-aggregometry-after-fibrinolysis
#11
Vincent Roule, Pierre Ardouin, Yohan Repessé, Agnès Le Querrec, Katrien Blanchart, Adrien Lemaitre, Rémi Sabatier, Annie Borel-Derlon, Farzin Beygui
Detection of high on-treatment platelet reactivity (HPR) by point-of-care tests has not been validated after successful fibrinolysis for ST-elevation myocardial infarction. We assessed the validity of the point-of-care VerifyNow P2Y12 (VN) and INNOVANCE PFA P2Y (PFA) tests on HPR compared to light transmittance aggregometry (LTA) in these patients. The HPR was identified in 10 (34.5%) patients, 15 (51.7%) patients, and 14 (50%) patients using LTA, VN, and PFA, respectively. Discrepancies were observed between the tests despite significant correlations between platelet reactivity measures by LTA and VN ( r = 0...
January 1, 2018: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/29716485/the-effects-of-haemodilution-with-succinylated-gelatin-solution-on-coagulation-in-vitro-as-assessed-by-thromboelastometry-and-impedance-multiple-electrode-aggregometry
#12
Pca Kam, S Varanasi, K X Yang
We investigated the in vitro viscoelastic changes of progressive haemodilution with succinylated gelatin (SG) solution compared with normal saline (NS) using rotational thromboelastometry (ROTEM®). Whole blood (WB) samples obtained from 20 healthy volunteers were diluted in vitro with SG solution or NS by 10%, 20% and 40%. Fibrinogen concentration and ROTEM (EXTEM, FIBTEM) variables including coagulation time (CT), clot formation time (CFT), α-angle, and maximum clot firmness (MCF) were measured in the undiluted sample and at each degree of haemodilution...
May 2018: Anaesthesia and Intensive Care
https://www.readbyqxmd.com/read/29715047/not-all-light-transmission-aggregation-assays-are-created-equal-qualitative-differences-between-light-transmission-and-96-well-plate-aggregometry
#13
Melissa V Chan, Philip D Leadbeater, Steve P Watson, Timothy D Warner
In this short article, submitted as part of the review on platelet function testing, we illustrate the quantitative and qualitative differences between classical light transmission aggregometry (LTA) and 96-well plate aggregometry. We show that responses to platelet agonists and antagonists differ depending upon the method of aggregation testing. For example, in 96-well aggregometry, responses to collagen are strongly inhibited by P2Y12 receptor antagonists while in LTA they are much less affected. Furthermore, we explore the importance of differences in the mechanical environment upon platelet aggregation...
May 1, 2018: Platelets
https://www.readbyqxmd.com/read/29710690/the-combined-effects-of-the-microcirculatory-status-and-cardiopulmonary-bypass-on-platelet-count-and-function-during-cardiac-surgery
#14
Umberto Di Dedda, Marco Ranucci, Alberto Porta, Vlasta Bari, Alice Ascari, Angela Fantinato, Ekaterina Baryshnikova, Mauro Cotza
BACKGROUND: Cardiac surgery with cardiopulmonary bypass is associated with important changes in the microcirculation, usually attributed to endothelial dysfunction. Another common finding of cardiac surgery is postoperative thrombocytopenia and platelet loss of function. OBJECTIVE: To investigate the association between microvascular flow pattern and postoperative changes in platelet count and function in cardiac surgery patients. METHODS: Twelve adult cardiac surgery patients received microvascular circulation (sidestream darkfield sublingual mucosa analysis) and platelet count and function (multiple electrode aggregometry ADPtest and TRAPtest) assessment before and after cardiopulmonary bypass...
April 14, 2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29708866/the-characterization-of-monoclonal-antibodies-to-mouse-tlt-1-suggests-that-tlt-1-plays-a-role-in-wound-healing
#15
Barbara Manfredi, Jessica Morales-Ortíz, Lymarie M Díaz-Díaz, Liz Hernandez-Matias, Delmaliz Barreto-Vázquez, Javier Menéndez-Pérez, J Alejandro Rodríguez-Cordero, Juan C Villalobos-Santos, Edgardo Santiago-Rivera, Adriana Rivera-Dompenciel, Eunice L Lozada-Delgado, Madhavi Kuchibhotla, Kelvin Carrasquillo-Carrión, Abiel Roche-Lima, A Valance Washington
Platelets play a vital role in hemostasis and inflammation. The membrane receptor TREM-like transcript-1 (TLT-1) is involved in platelet aggregation, bleeding, and inflammation, and it is localized in the α-granules of platelets. Upon platelet activation, TLT-1 is released from α-granules both in its transmembrane form and as a soluble fragment (sTLT-1). Higher levels of sTLT-1 have been detected in the plasma of patients with acute inflammation or sepsis, suggesting an important role for TLT-1 during inflammation...
April 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29704268/characteristics-of-hemostasis-during-experimental-ehrlichia-canis-infection
#16
Sarah Shropshire, Christine Olver, Michael Lappin
BACKGROUND: Ehrlichia canis infection in dogs can cause thrombocytopenia and clinical evidence of bleeding. It is unknown why some dogs show signs of bleeding whereas others do not despite clinically relevant thrombocytopenia. HYPOTHESIS/OBJECTIVES: Activated platelets, decreased fibrinolysis or both mitigate bleeding tendency. Assess standard hemostatic variables, platelet dynamics, and specialized coagulation testing in dogs experimentally infected with E. canis to evaluate this clinical discrepancy...
April 27, 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29671861/p2y12-antagonist-ticagrelor-inhibits-the-release-of-procoagulant-extracellular-vesicles-from-activated-platelets-preliminary-results
#17
Aleksandra Gasecka, Rienk Nieuwland, Edwin van der Pol, Najat Hajji, Agata Ćwiek, Kinga Pluta, Michał Konwerski, Krzysztof J Filipiak
BACKGROUND: Activated platelets release platelet extracellular vesicles (PEVs). Adenosine diphosphate (ADP) receptors P2Y1 and P2Y12 both play a role in platelet activation, The present hypothesis herein is that the inhibition of these receptors may affect the release of PEVs. METHODS: Platelet-rich plasma from 10 healthy subjects was incubated with saline, P2Y1 antagonist MRS2179 (100 µM), P2Y12 antagonist ticagrelor (1 µM), and a combination of both antagonists...
April 19, 2018: Cardiology Journal
https://www.readbyqxmd.com/read/29669386/the-essential-ectoenzyme-smnpp5-from-the-human-intravascular-parasite-schistosoma-mansoni-is-an-adpase-and-a-potent-inhibitor-of-platelet-aggregation
#18
Manal Elzoheiry, Akram A Da'dara, Armelle M deLaforcade, Samar N El-Beshbishi, Patrick J Skelly
Schistosomes are intravascular parasitic platyhelminthes infecting > 200 million people globally and causing a debilitating disease, schistosomiasis. Despite the relatively large size of the adult worms and their disruption of blood flow, surprisingly, they do not appear to provoke thrombus formation around them in vivo. We hypothesize that proteins expressed at the host-parasite interface are key to this ability. Here, we functionally express an ectonucleotide pyrophosphatase/phosphodiesterase homologue, SmNPP5, that is expressed at the tegumental surface of intravascular Schistosoma mansoni ...
April 18, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29652280/relationship-between-maximum-clot-firmness-in-rotem-%C3%A2-and-postoperative-bleeding-after-coronary-artery-bypass-graft-surgery-in-patients-using-clopidogrel
#19
Rasoul Azarfarin, Fereidoon Noohi, Majid Kiavar, Ziae Totonchi, Avaz Heidarpour, Amir Hendiani, Zahra Sadat Koleini, Saeid Rahimi
Background: The aim of the present study was to investigate the relationship between maximum clot firmness (MCF) in rotational thromboelastometry (ROTEM® ) and postoperative bleeding in patients on clopidogrel after emergency coronary artery bypass graft surgery (CABG). Methods: This observational study recruited 60 patients posted for emergency CABG following unsuccessful primary percutaneous coronary intervention (PCI) while on 600 mg of clopidogrel. The study population was divided into 2 groups on the basis of their MCF in the extrinsically activated thromboelastometric (EXTEM) component of the (preoperative) ROTEM® test: patients with MCF <50 mm (n = 16) and those with MCF ≥50 mm (n = 44)...
April 2018: Annals of Cardiac Anaesthesia
https://www.readbyqxmd.com/read/29650295/acquired-von-willebrand-syndrome-and-impaired-platelet-function-during-venovenous-extracorporeal-membrane-oxygenation-rapid-onset-and-fast-recovery
#20
Johannes Kalbhenn, Axel Schlagenhauf, Simone Rosenfelder, Axel Schmutz, Barbara Zieger
BACKGROUND: Bleeding contributes to the high mortality of venovenous extracorporeal membrane oxygenation (vvECMO). The development of acquired von Willebrand syndrome (AVWS) has been identified as relevant pathology during ECMO. This study was performed to determine the onset of AVWS after implantation and the recovery of von Willebrand factor (VWF) parameters after explantation of ECMO in a large cohort of patients. METHODS: VWF parameters of 59 patients treated with vvECMO at a university ECMO center were obtained before ECMO implantation, during therapy, and after explantation...
March 17, 2018: Journal of Heart and Lung Transplantation
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