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https://www.readbyqxmd.com/read/29337406/validation-of-flow-cytometric-analysis-of-platelet-function-in-patients-with-a-suspected-platelet-function-defect
#1
Ivar van Asten, Roger E G Schutgens, Marije Baaij, Judith Zandstra, Mark Roest, Gerard Pasterkamp, Albert Huisman, Suzanne J A Korporaal, Rolf T Urbanus
BACKGROUND: Light transmission aggregometry (LTA) is the most commonly used test for the diagnosis of platelet function disorders (PFDs), but has moderate sensitivity for mild PFDs. Flow cytometry has been recommended for additional diagnostics of PFDs but is not yet standardized as a diagnostic test. We developed a standardized protocol for flow cytometric analysis of platelet function that measures fibrinogen binding and P-selectin expression as platelet activation markers in response to agonist stimulation...
January 16, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29336311/comparison-of-the-effects-of-p2y12-receptor-antagonists-on-platelet-function-and-clinical-outcomes-in-patients-undergoing-primary-pci-a-substudy-of-the-heat-ppci-trial
#2
Adeel Shahzad, Vikram Khanna, Ian Kemp, Matthew Shaw, Christine Mars, Robert Cooper, Keith Wilson, Nick Curzen, Rod H Stables
AIMS: The HEAT-PPCI trial compared bivalirudin and unfractionated heparin in patients undergoing Primary PCI. We report pre-specified, secondary analyses comparing the effects of P2Y12 inhibiting agents on platelet reactivity and clinical events. METHODS AND RESULTS: All patients received pre-procedural oral anti-platelet therapy. During the early stages of the trial, the P2Y12 inhibitor of choice was prasugrel with some use of clopidogrel. Later, routine therapy switched to ticagrelor...
January 16, 2018: EuroIntervention
https://www.readbyqxmd.com/read/29334682/body-mass-index-and-plasma-p-selectin-before-coronary-stenting-predict-high-residual-platelet-reactivity-at-6-months-on-dual-antiplatelet-therapy
#3
Elena Z Golukhova, Marina V Grigoryan, Mariya N Ryabinina, Naida I Bulaeva, Victor L Serebruany
BACKGROUND: High residual platelet reactivity (HRPR) during dual antiplatelet therapy (DAPT) may impact clinical outcomes following percutaneous coronary interventions (PCI). However, whether any biomarkers assessed before PCI at DAPT loading may predict delayed maintenance HRPR is not clear. OBJECTIVE: The aim of this study was to determine whether conventional clinical or laboratory indices at loading before stenting may predict HRPR at 6 months of maintenance DAPT...
January 16, 2018: Cardiology
https://www.readbyqxmd.com/read/29306729/adding-to-the-complexity-of-fetal-and-neonatal-alloimmune-thrombocytopenia-reduced-fibrinogen-binding-in-the-presence-of-anti-hpa-1a-antibody-and-hypo-responsive-neonatal-platelets
#4
E Refsum, S Meinke, G Gryfelt, A Wikman, P Höglund
BACKGROUND: In fetal and neonatal alloimmune thrombocytopenia (FNAIT), maternal alloantibodies directed against paternally-derived platelet antigens are transported across the placenta to the fetus, where they may cause thrombocytopenia. The most serious complication of FNAIT is an intracranial hemorrhage (ICH), which may cause death or life-long disability of the child. Apart from alloantibody-mediated platelet destruction, the clinical outcome in FNAIT may be affected by properties of neonatal platelets and possible functional effects on platelets caused by maternal alloantibodies...
December 29, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/29304531/dietary-nitrate-supplementation-reduces-circulating-platelet-derived-extracellular-vesicles-in-coronary-artery-disease-patients-on-clopidogrel-therapy-a-randomised-double-blind-placebo-controlled-study
#5
Nicholas Burnley-Hall, Fairoz Abdul, Vitaliy Androshchuk, Keith Morris, Nick Ossei-Gerning, Richard Anderson, D Aled Rees, Philip E James
Extracellular vesicles (EVs) are implicated in the pathogenesis of cardiovascular disease (CVD). Specifically, platelet-derived EVs are highly pro-coagulant, promoting thrombin generation and fibrin clot formation. Nitrate supplementation exerts beneficial effects in CVD, via an increase in nitric oxide (NO) bioavailability. Clopidogrel is capable of producing NO-donating compounds, such as S-nitrosothiols (RSNO) in the presence of nitrite and low pH. The aim of this study was to assess the effect of nitrate supplementation with versus without clopidogrel therapy on circulating EVs in coronary artery disease (CAD) patients...
January 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29304392/aspirin-non-responsiveness-in-pregnant-women-at-high-risk-of-pre-eclampsia
#6
Kate Navaratnam, Ana Alfirevic, Andrea Jorgensen, Zarko Alfirevic
OBJECTIVES: Low-dose aspirin is recommended for prevention of pre-eclampsia in high-risk pregnant women. Current doses provide a conservative risk reduction and some individuals demonstrate 'aspirin non-responsiveness', with insufficient antiplatelet effects. We aimed to determine if aspirin non-responsiveness could be identified in women at high risk of pre-eclampsia and assess for potential associations with placentally-mediated adverse outcomes. STUDY DESIGN: Prospective cohort study...
January 1, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29297721/mucor-circinelloides-induces-platelet-aggregation-through-integrin-%C3%AE-iib%C3%AE-3-and-fc%C3%AE-riia
#7
Harlene Ghuman, Alicia Shepherd-Roberts, Stephanie Watson, Malou Zuidscherwoude, Steve P Watson, Kerstin Voelz
Thrombosis is a hallmark of the fatal fungal infection mucormycosis. Yet, the platelet activation pathway in response to mucormycetes is unknown. In this study we determined the platelet aggregation potential of Mucor circinelloides (M. circinelloides) NRRL3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. Using impedance and light-transmission aggregometry, we showed that M...
January 3, 2018: Platelets
https://www.readbyqxmd.com/read/29296914/severe-platelet-dysfunction-in-nhl-patients-receiving-ibrutinib-is-absent-in-patients-receiving-acalabrutinib
#8
Alexander P Bye, Amanda J Unsworth, Michael J Desborough, Catherine A T Hildyard, Niamh Appleby, David Bruce, Neline Kriek, Sophie H Nock, Tanya Sage, Craig E Hughes, Jonathan M Gibbins
The Bruton tyrosine kinase (Btk) inhibitor ibrutinib induces platelet dysfunction and causes increased risk of bleeding. Off-target inhibition of Tec is believed to contribute to platelet dysfunction and other side effects of ibrutinib. The second-generation Btk inhibitor acalabrutinib was developed with improved specificity for Btk over Tec. We investigated platelet function in patients with non-Hodgkin lymphoma (NHL) receiving ibrutinib or acalabrutinib by aggregometry and by measuring thrombus formation on collagen under arterial shear...
December 12, 2017: Blood Advances
https://www.readbyqxmd.com/read/29242303/ibrutinib-does-not-affect-ristocetin-induced-platelet-aggregation-evaluated-by-light-transmission-aggregometry-in-chronic-lymphocytic-leukemia-patients
#9
Maria Adele Alberelli, Idanna Innocenti, Francesco Autore, Luca Laurenti, Erica De Candia
No abstract text is available yet for this article.
December 14, 2017: Haematologica
https://www.readbyqxmd.com/read/29240268/assessment-of-procoagulant-potential-in-patients-with-reactive-thrombocytosis-and-its-association-with-platelet-count
#10
Amihai Rottenstreich, Ela Shai, Geffen Kleinstern, Galia Spectre, David Varon, Yosef Kalish
OBJECTIVE: We aimed to determine hemostatic changes and characterize the procoagulant potential among reactive thrombocytosis (RT) patients. METHODS: Sixty RT patients (median platelet count 718X109 /L) and 20 healthy persons were tested for complete blood count, C-reactive protein, von Willebrand factor (VWF), factor VIII and fibrinogen, and thrombin generation. Platelet studies, including light transmission aggregometry and Cone and Plate(let) Analyzer, were also conducted...
December 14, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/29219795/-platelet-function-tests-tailored-antiplatelet-therapy-for-vascular-patients
#11
A R T Brand, S J A Korporaal, R T Urbanus, G Pasterkamp, G J de Borst
- Platelet aggregation inhibitors, also known as antiplatelet therapy (APT), are prescribed for the prevention of secondary cardiovascular events (CVE) after endovascular revascularization procedures.- Platelet aggregation inhibitors are not equally effective in all patients. The phenomenon of high residual platelet reactivity despite APT is called 'high on-treatment platelet reactivity' (HTPR); it bears an increased risk of secondary CVE.- Platelet function tests (PFT) can be used to diagnose HTPR. There are various tests available; of those, light transmission aggregometry (LTA) is considered the gold standard...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/29216784/evaluation-of-peripheral-serotonin-content-and-%C3%AE-2-adrenergic-receptor-function-as-potential-markers-for-life-long-recurrent-depressive-disorder-by-using-methodological-improvements
#12
Boris Aleksovski, Antoni Novotni, Viktorija Vujović, Vladimir Rendevski, Nensi Manusheva, Violeta Neceva, Ana Filipce, Aleksandar Risteski, Vasko Aleksovski, Icko Gjorgoski
OBJECTIVE: We aimed to evaluate the role and the relations between peripheral platelet serotonin content, blood plasma serotonin concentration and the function of platelet α2-adrenergic receptors (α2-AR) as potential state or trait biomarkers for recurrent depressive disorder (RDD). METHODS: 26 drug-free patients with life-long RDD and 31 healthy controls were included in the study. Several methodological improvements in blood collection and platelet isolation were implemented following the present standards in Haematology and Light transmission aggregometry...
December 7, 2017: International Journal of Psychiatry in Clinical Practice
https://www.readbyqxmd.com/read/29214676/platelet-function-monitoring-for-stent-thrombosis-in-critically-iii-patients-with-an-acute-coronary-syndrome
#13
Lisa Tilemann, Sarah K Mohr, Michael Preusch, Emanuel Chorianopoulos, Evangelos Giannitsis, Hugo A Katus, Oliver J Müller
BACKGROUND: Patients after cardiac arrest or in cardiogenic shock due to acute coronary syndrome (ACS) are at high risk for stent thrombosis (ST) and recurrent cardiovascular events after primary percutaneous coronary intervention (PCI). High post-interventional platelet reactivity (HPR) might be an additional risk factor for ST in these critically ill patients. METHODS: Between 2006 and 2016, 401 critically ill patients from a cardiologic intensive care unit underwent platelet function testing after primary PCI using whole blood impedance aggregometry...
December 6, 2017: Journal of Interventional Cardiology
https://www.readbyqxmd.com/read/29203584/fc-independent-immune-thrombocytopenia-via-mechanomolecular-signaling-in-platelets
#14
M Edward Quach, Matthew A Dragovich, Wenchun Chen, Anum K Syed, Wenpeng Cao, Xin Liang, Wei Deng, Simon F De Meyer, Guangheng Zhu, Jun Peng, Heyu Ni, Carolyn M Bennett, Ming Hou, Jerry Ware, Hans Deckmyn, X Frank Zhang, Renhao Li
Immune thrombocytopenia (ITP) is a prevalent autoimmune disease characterized by autoantibody-induced platelet clearance. Some ITP patients are refractory to standard immunosuppressive treatments such as intravenous immunoglobulin (IVIG). These patients often have autoantibodies targeting the ligand-binding domain (LBD) of glycoprotein (GP)Ibα, a major subunit of the platelet mechanoreceptor complex GPIb-IX. However, the molecular mechanism of this Fc-independent platelet clearance is not clear. Here, we report that many anti-LBD monoclonal antibodies such as 6B4, but not AK2, activated GPIb-IX in a shear-dependent manner and induced IVIG-resistant platelet clearance in mice...
December 4, 2017: Blood
https://www.readbyqxmd.com/read/29185836/exome-chip-meta-analysis-identifies-association-between-variation-in-ankrd26-and-platelet-aggregation
#15
Ming-Huei Chen, Lisa R Yanek, Joshua D Backman, John D Eicher, Jennifer E Huffman, Yoav Ben-Shlomo, Andrew D Beswick, Laura M Yerges-Armstrong, Alan R Shuldiner, Jeffrey R O'Connell, Rasika A Mathias, Diane M Becker, Lewis C Becker, Joshua P Lewis, Andrew D Johnson, Nauder Faraday
Previous genome-wide association studies (GWAS) have identified several variants associated with platelet function phenotypes; however, the proportion of variance explained by the identified variants is mostly small. Rare coding variants, particularly those with high potential for impact on protein structure/function, may have substantial impact on phenotype but are difficult to detect by GWAS. The main purpose of this study was to identify low frequency or rare variants associated with platelet function using genotype data from the Illumina HumanExome Bead Chip...
November 29, 2017: Platelets
https://www.readbyqxmd.com/read/29182470/high-shear-induces-platelet-dysfunction-leading-to-enhanced-thrombotic-propensity-and-diminished-hemostatic-capacity
#16
Zengsheng Chen, Nandan K Mondal, Shirong Zheng, Steven C Koenig, Mark S Slaughter, Bartley P Griffith, Zhongjun J Wu
Thrombosis and bleeding are devastating adverse events in patients supported with blood-contacting medical devices (BCMDs). In this study, we delineated that high non-physiological shear stress (NPSS) caused platelet dysfunction that may contribute to both thrombosis and bleeding. Human blood was subjected to NPSS with short exposure time. Levels of platelet surface GPIbα and GPVI receptors as well as activation level of GPIIb/IIIa in NPSS-sheared blood were examined with flow cytometry. Adhesion of sheared platelets on fibrinogen, von Willibrand factor (VWF), and collagen was quantified with fluorescent microscopy...
November 28, 2017: Platelets
https://www.readbyqxmd.com/read/29182390/in-vitro-effect-of-blood-cell-counts-on-multiple-electrode-impedance-aggregometry-in-dogs
#17
Katherine J Nash, Lenore M Bacek, Pete W Christopherson, Elizabeth A Spangler
OBJECTIVE To assess the effect of decreased platelet and WBC counts on platelet aggregation as measured by a multiple-electrode impedance aggregometer in dogs. ANIMALS 24 healthy dogs. PROCEDURES From each dog, 9 mL of blood was collected into a 10-mL syringe that contained 1 mL of 4% sodium citrate solution to yield a 10-mL sample with a 1:9 citrate-to-blood ratio. Each sample was then divided into unmanipulated and manipulated aliquots with progressively depleted buffy-coat fractions such that 2 to 3 blood samples were evaluated per dog...
December 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29177885/clopidogrel-therapy-in-patients-with-cardiovascular-disease-undergoing-transurethral-resection-of-the-prostate-a-step-towards-individualization
#18
Petros Tzimas, Maria Tsoumani, Dimitrios Giannakis, Kallirroi Kalantzi, Anastasios Petrou, Vasileios Chantzichristos, Nikolaos Sofikitis, Georgios Papadopoulos, Haralampos Milionis, Alexandros Tselepis
OBJECTIVES: The objective of this study was to test platelet function pre- and peri-operatively in clopidogrel-treated patients undergoing transurethral resection of the prostate. METHODS: This was a pilot study involving 20 male patients treated with clopidogrel (75 mg/day) for the secondary prevention of cardiovascular disease and scheduled for elective transurethral resection of the prostate. Platelet function testing with light transmittance aggregometry in platelet-rich plasma of four samples (T0, T1, T2, and T3 drawn on the same day, 3 and 7 days of clopidogrel cessation and 24-h post-operatively, respectively) was performed and evaluated in each patient...
November 25, 2017: Drugs & Aging
https://www.readbyqxmd.com/read/29175435/antiplatelet-effect-of-aspirin-during-24h-in-patients-with-type-2-diabetes-without-cardiovascular-disease
#19
Liv Vernstrøm, Kristian Løkke Funck, Erik Lerkevang Grove, Esben Laugesen, Jonathan Mathias Baier, Anne-Mette Hvas, Per Løgstrup Poulsen
INTRODUCTION: The antiplatelet effect of low-dose aspirin in patients with type 2 diabetes (T2DM) without cardiovascular disease (CVD) has not been thoroughly explored. We investigated if platelet aggregation increased during the standard 24-hour aspirin dosing interval in patients with T2DM compared to non-diabetic controls. Furthermore, we evaluated baseline platelet aggregation, the acute effects of aspirin on platelet aggregation and platelet turnover. MATERIALS AND METHODS: We included 21 patients with T2DM and 21 age and sex-matched controls...
November 20, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/29171876/decreased-platelet-inhibition-by-p2y12-receptor-blockers-in-anemia
#20
Patricia P Wadowski, Christoph W Kopp, Renate Koppensteiner, Irene M Lang, Joseph Pultar, Silvia Lee, Constantin Weikert, Simon Panzer, Thomas Gremmel
BACKGROUND: Anemic patients undergoing angioplasty and stenting are at an increased risk of ischemic events, which may be caused by an inadequate response to antiplatelet therapy with adenosine diphosphate (ADP) P2Y12 inhibitors. In the current study, we investigated the associations between anemia and on-treatment platelet reactivity in clopidogrel- (group 1, n=306) and prasugrel-/ticagrelor-treated (group 2, n=109) patients undergoing elective and acute angioplasty with stent implantation, respectively...
November 24, 2017: European Journal of Clinical Investigation
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