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https://www.readbyqxmd.com/read/28518080/rearing-ixodes-scapularis-the-black-legged-tick-feeding-immature-stages-on-mice
#1
Andrew B Nuss, Manoj G Mathew, Monika Gulia-Nuss
Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a three host tick that requires a blood meal from a vertebrate host for each development stage, and the adult females require a blood meal for reproduction. Larval ticks attach to their host for 3 - 5 days for feeding and drop off the host when fully engorged. This dependency on several different hosts and the lengthy attachment time for engorgement complicates tick rearing in the laboratory setting...
May 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28508918/-local-anaesthesia-in-ruminants
#2
Karl Nuss, Andrea Schwarz, Simone Ringer
The use of local anaesthesia in ruminants allows many surgical procedures to be conducted free of pain, efficiently and inexpensively in the field. Local anaesthesia combined with sedation and immobilisation of the animal can replace general anaesthesia for many procedures (e. g. castration, claw amputation). The level of difficulty differs among various local anaesthetic techniques: local infiltration of tissue or anaesthesia of the cornual nerve are easily performed, whereas local anaesthesia of the eye, regional anaesthesia in limbs or anaesthesia for umbilical surgery are more difficult to carry out...
May 16, 2017: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/28502740/isolation-of-an-insulin-like-peptide-from-the-asian-malaria-mosquito-anopheles-stephensi-that-acts-as-a-steroidogenic-gonadotropin-across-diverse-mosquito-taxa
#3
Andrew B Nuss, Mark R Brown
Many insulin-like peptides (ILPs) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. Antiserum produced to ILP3 in Aedes aegypti was used in a radioimmunoassay to monitor the purification of an ILP from heads of adult An. stephensi and recognized the ILP in other immunoassays. The structure of the purified peptide matched that predicted for the ILP3 in this species. The native form stimulated ecdysteroid production by ovaries isolated from non-blood fed females...
May 11, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28485603/light-mediated-preparation-of-palladium-nanoparticles-as-catalysts-for-alkyne-cis-semihydrogenation
#4
Florian Mäsing, Harald Nüsse, Jürgen Klingauf, Armido Studer
A bisacylphosphine oxide photoinitiator was used for the light mediated preparation of palladium nanoparticles (PdNPs) with a small diameter of 2.8 nm. All starting materials are commercially available, and PdNP synthesis is experimentally very easy to conduct. The PdNP-hybrid material was applied as catalyst for the semihydrogenation of various internal alkynes to provide the corresponding alkenes in excellent yields (up to 99%) and Z-selectivities (Z/E ratios up to 99/1).
May 9, 2017: Organic Letters
https://www.readbyqxmd.com/read/28485535/self-assembly-of-redox-responsive-polymer-nanocontainers-for-intracellular-delivery-by-a-reversible-supramolecular-stabilization-of-vesicles
#5
Wilke de Vries, David Grill, Matthias Tesch, Andrea Ricker, Harald Nüsse, Jürgen Klingauf, Armido Studer, Volker Gerke, Bart Jan Ravoo
We present self-assembly of redox-responsive polymer nanocontainers comprising a cyclodextrin vesicle core and a thin reductively cleavable polymer shell anchored via host-guest recognition on the vesicle surface. The nanocontainers are of uniform size, show high stability and selectively respond to a mild reductive trigger as revealed by dynamic light scattering, transmission electron microscopy, atomic force microscopy, a quantitative thiol assay and fluorescence spectroscopy. Live cell imaging experiments demonstrate a specific redox-responsive release and cytoplasmic delivery of encapsulated hydrophilic payloads such as the pH-probe pyranine, and the fungal toxin phalloidin...
May 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28445085/regenerative-surgery-in-the-treatment-of-cosmetic-defect-following-nuss-procedure
#6
Flavio Facchini, Marco Ghionzoli, Alessandra Martin, Sara Tanini, Sara Ugolini, Roberto Lo Piccolo, Antonio Messineo
BACKGROUND: In the past decade, the minimally invasive repair of pectus excavatum (MIRPE) has become the treatment of choice for severe pectus excavatum (PE), proving acceptable to excellent cosmetic results. Recently, autologous fat grafting (FG) has been identified in aesthetic and reconstructive surgery to ideally handle volume and contour defects. We report our experience about FG in the treatment of residual minor defects after MIRPE and a proposal for a new indication of this largely adopted technique...
April 26, 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/28440717/serum-carotenoid-interactions-in-premenopausal-women-reveal-%C3%AE-carotene-is-negatively-impacted-by-body-fat
#7
Emily Taylor Nuss, Ashley R Valentine, Zhumin Zhang, HuiChuan Jennifer Lai, Sherry A Tanumihardjo
Increasing body mass indices (BMIs) across the globe reflect pandemic shifts towards habitual positive energy imbalances. Excess body fat in individuals is often associated with high-energy and high-fat diets scanty in fresh produce. Carotenoids are fat-soluble pigments plentiful in many fruits and vegetables. They are well-known for provitamin A and antioxidant functions, but little research has been done related to carotenoid-body mass interactions. Serum carotenoids were analyzed relative to body fat to determine correlations between major serum carotenoids, retinol, BMI, fat mass, and lean mass...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28366437/mechanical-histological-and-functional-properties-remain-inferior-in-conservatively-treated-achilles-tendons-in-rodents-long-term-evaluation
#8
Benjamin R Freedman, George W Fryhofer, Nabeel S Salka, Harina A Raja, Cody D Hillin, Courtney A Nuss, Daniel C Farber, Louis J Soslowsky
Conservative treatment (non-operative) of Achilles tendon ruptures is suggested to produce equivalent capacity for return to function; however, long term results and the role of return to activity (RTA) for this treatment paradigm remain unclear. Therefore, the objective of this study was to evaluate the long term response of conservatively treated Achilles tendons in rodents with varied RTA. Sprague Dawley rats (n=32) received unilateral blunt transection of the Achilles tendon followed by randomization into groups that returned to activity after 1-week (RTA1) or 3-weeks (RTA3) of limb casting in plantarflexion, before being euthanized at 16-weeks post-injury...
May 3, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28364483/ecomment-the-nuss-technique-a-track-full-of-hazards
#9
Alain J Wurtz, Emmanuel Brian
No abstract text is available yet for this article.
February 1, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28341230/intraoperative-cryoanalgesia-for-managing-pain-after-the-nuss-procedure
#10
Claire Graves, Olajire Idowu, Sang Lee, Benjamin Padilla, Sunghoon Kim
BACKGROUND: Cryoanalgesia prevents pain by freezing the affected peripheral nerve. We report the use of intraoperative cryoanalgesia during the Nuss procedure for pectus excavatum and describe our initial experience, modifications of technique, and lessons learned. MATERIALS AND METHODS: We retrospectively reviewed the medical records of patients who received cryoanalgesia during the Nuss procedure between June 1, 2015, and April 30, 2016, at our institutions and analyzed modifications in surgical technique during this early adoption period...
March 16, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28329174/ccr7-deficiency-modulates-t-cell-response-and-increases-susceptibility-to-yersinia-pseudotuberculosis-infection
#11
Joern Pezoldt, Fabio Pisano, Wiebke Heine, Maria Pasztoi, Maik Rosenheinrich, Aaron M Nuss, Marina C Pils, Immo Prinz, Reinhold Förster, Jochen Huehn, Petra Dersch
Background: To successfully limit pathogen dissemination an immunological link between the entry tissue of the pathogen and the underlying secondary lymphoid organs (SLO) needs to be established to prime adaptive immune responses. Here, the prerequisite of CCR7 to mount host immune responses within SLOs during gastrointestinal Yersinia pseudotuberculosis infection to limit pathogen spread was investigated. Methods: Survival, bacterial dissemination, intestinal and systemic pathology of wild type (WT) and CCR7-/- mice were assessed and correlated to the presence of immune cell subsets and cytokine responses throughout the course of infection...
January 27, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28328947/a-spontaneous-mutation-in-kdsd-a-biosynthesis-gene-for-3-deoxy-d-manno-octulosonic-acid-occurred-in-a-ciprofloxacin-resistant-strain-of-francisella-tularensis-and-caused-a-high-level-of-attenuation-in-murine-models-of-tularemia
#12
Taylor Chance, Jennifer Chua, Ronald G Toothman, Jason T Ladner, Jonathan E Nuss, Jo Lynne Raymond, Fabrice V Biot, Samandra Demons, Lynda Miller, Stephanie Halasohoris, Sherry Mou, Galina Koroleva, Sean Lovett, Gustavo Palacios, Nicholas J Vietri, Patricia L Worsham, Christopher K Cote, Todd M Kijek, Joel A Bozue
Francisella tularensis, a gram-negative facultative intracellular bacterial pathogen, is the causative agent of tularemia and able to infect many mammalian species, including humans. Because of its ability to cause a lethal infection, low infectious dose, and aerosolizable nature, F. tularensis subspecies tularensis is considered a potential biowarfare agent. Due to its in vitro efficacy, ciprofloxacin is one of the antibiotics recommended for post-exposure prophylaxis of tularemia. In order to identify therapeutics that will be efficacious against infections caused by drug resistant select-agents and to better understand the threat, we sought to characterize an existing ciprofloxacin resistant (CipR) mutant in the Schu S4 strain of F...
2017: PloS One
https://www.readbyqxmd.com/read/28306280/search-for-cosmic-ray-electron-and-positron-anisotropies-with-seven-years-of-fermi-large-area-telescope-data
#13
S Abdollahi, M Ackermann, M Ajello, A Albert, W B Atwood, L Baldini, G Barbiellini, R Bellazzini, E Bissaldi, E D Bloom, R Bonino, E Bottacini, T J Brandt, P Bruel, S Buson, M Caragiulo, E Cavazzuti, A Chekhtman, S Ciprini, F Costanza, A Cuoco, S Cutini, F D'Ammando, F de Palma, R Desiante, S W Digel, N Di Lalla, M Di Mauro, L Di Venere, B Donaggio, P S Drell, C Favuzzi, W B Focke, Y Fukazawa, S Funk, P Fusco, F Gargano, D Gasparrini, N Giglietto, F Giordano, M Giroletti, D Green, S Guiriec, A K Harding, T Jogler, G Jóhannesson, T Kamae, M Kuss, S Larsson, L Latronico, J Li, F Longo, F Loparco, P Lubrano, J D Magill, D Malyshev, A Manfreda, M N Mazziotta, M Meehan, P F Michelson, W Mitthumsiri, T Mizuno, A A Moiseev, M E Monzani, A Morselli, M Negro, E Nuss, T Ohsugi, N Omodei, D Paneque, J S Perkins, M Pesce-Rollins, F Piron, G Pivato, G Principe, S Rainò, R Rando, M Razzano, A Reimer, O Reimer, C Sgrò, D Simone, E J Siskind, F Spada, G Spandre, P Spinelli, A W Strong, H Tajima, J B Thayer, D F Torres, E Troja, J Vandenbroucke, G Zaharijas, S Zimmer
The Large Area Telescope on board the Fermi Gamma-ray Space Telescope has collected the largest ever sample of high-energy cosmic-ray electron and positron events since the beginning of its operation. Potential anisotropies in the arrival directions of cosmic-ray electrons or positrons could be a signature of the presence of nearby sources. We use almost seven years of data with energies above 42 GeV processed with the Pass 8 reconstruction. The present data sample can probe dipole anisotropies down to a level of 10^{-3}...
March 3, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28280644/vitamin-k-deficiency-presenting-in-an-infant-with-an-anterior-mediastinal-mass-a-case-report-and-review-of-the-literature
#14
Mauricio A Palau, Amanda Winters, Xiayuan Liang, Rachelle Nuss, Susan Niermeyer, Megan Gossling, Clyde Wright
We report a case of a 1-month-old infant with spontaneous thymic hemorrhage secondary to severe vitamin K deficiency. He was brought to medical attention due to scrotal bruising and during evaluation was noted to be tachypneic and hypoxemic. Chest X-ray revealed an enlarged cardiothymic silhouette, and a follow-up echocardiogram revealed a mass in the anterior mediastinum. Routine laboratory work-up revealed severe coagulopathy. Further questioning revealed the patient had not received prophylactic vitamin K at birth...
2017: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/28265087/classroom-sound-can-be-used-to-classify-teaching-practices-in-college-science-courses
#15
Melinda T Owens, Shannon B Seidel, Mike Wong, Travis E Bejines, Susanne Lietz, Joseph R Perez, Shangheng Sit, Zahur-Saleh Subedar, Gigi N Acker, Susan F Akana, Brad Balukjian, Hilary P Benton, J R Blair, Segal M Boaz, Katharyn E Boyer, Jason B Bram, Laura W Burrus, Dana T Byrd, Natalia Caporale, Edward J Carpenter, Yee-Hung Mark Chan, Lily Chen, Amy Chovnick, Diana S Chu, Bryan K Clarkson, Sara E Cooper, Catherine Creech, Karen D Crow, José R de la Torre, Wilfred F Denetclaw, Kathleen E Duncan, Amy S Edwards, Karen L Erickson, Megumi Fuse, Joseph J Gorga, Brinda Govindan, L Jeanette Green, Paul Z Hankamp, Holly E Harris, Zheng-Hui He, Stephen Ingalls, Peter D Ingmire, J Rebecca Jacobs, Mark Kamakea, Rhea R Kimpo, Jonathan D Knight, Sara K Krause, Lori E Krueger, Terrye L Light, Lance Lund, Leticia M Márquez-Magaña, Briana K McCarthy, Linda J McPheron, Vanessa C Miller-Sims, Christopher A Moffatt, Pamela C Muick, Paul H Nagami, Gloria L Nusse, Kristine M Okimura, Sally G Pasion, Robert Patterson, Pleuni S Pennings, Blake Riggs, Joseph Romeo, Scott W Roy, Tatiane Russo-Tait, Lisa M Schultheis, Lakshmikanta Sengupta, Rachel Small, Greg S Spicer, Jonathon H Stillman, Andrea Swei, Jennifer M Wade, Steven B Waters, Steven L Weinstein, Julia K Willsie, Diana W Wright, Colin D Harrison, Loretta A Kelley, Gloriana Trujillo, Carmen R Domingo, Jeffrey N Schinske, Kimberly D Tanner
Active-learning pedagogies have been repeatedly demonstrated to produce superior learning gains with large effect sizes compared with lecture-based pedagogies. Shifting large numbers of college science, technology, engineering, and mathematics (STEM) faculty to include any active learning in their teaching may retain and more effectively educate far more students than having a few faculty completely transform their teaching, but the extent to which STEM faculty are changing their teaching methods is unclear...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28264019/correction-hemoglobin-induced-cell-trauma-indirectly-influences-endothelial-tlr9-activity-resulting-in-pulmonary-vascular-smooth-muscle-cell-activation
#16
Zoe Loomis, Paul Eigenberger, Katherine Redinius, Christina Lisk, Vijaya Karoor, Eva Nozik-Grayck, Scott K Ferguson, Kathryn Hassell, Rachelle Nuss, Kurt R Stenmark, Paul W Buehler, David C Irwin
[This corrects the article DOI: 10.1371/journal.pone.0171219.].
2017: PloS One
https://www.readbyqxmd.com/read/28174144/eating-and-rumination-behaviour-in-cows-with-traumatic-reticuloperitonitis
#17
U Braun, T Tschoner, M Hässig, K Nuss
Eating and rumination behaviour in 22 cows with traumatic reticuloperitonitis was recorded after the start of treatment. Based on the results of clinical, ultrasonographic and radiographic examinations, the cows were divided into two groups: Group A consisted of 12 cows without an intramural or perforating foreign body and group B had 10 cows with an intramural or perforating foreign body. Cows of group A received amoxicillin, flunixin meglumine and a NaCl-glucose infusion, and cows of group B received the same treatment accompanied by foreign body removal via ruminotomy...
February 2017: Schweizer Archiv Für Tierheilkunde
https://www.readbyqxmd.com/read/28166820/case-report-clinical-and-postmortem-findings-in-four-cows-with-rib-fracture
#18
Ueli Braun, Sonja Warislohner, Udo Hetzel, Karl Nuss
BACKGROUND: Published reports of rib fractures in adult cattle are limited to the occurrence of chronic rib swellings caused by calluses, which are unremarkable from a clinical standpoint, whereas studies identifying clinical signs of rib fractures were not found in a literature search. This report describes the clinical and postmortem findings in four cows with rib fractures. CASE PRESENTATION: The 13th rib was fractured in three cows and the 11th rib in the remaining cow; three fractures were on the right and one on the left side...
February 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28152051/hemoglobin-induced-cell-trauma-indirectly-influences-endothelial-tlr9-activity-resulting-in-pulmonary-vascular-smooth-muscle-cell-activation
#19
Zoe Loomis, Paul Eigenberger, Katherine Redinius, Christina Lisk, Vijaya Karoor, Eva Nozik-Grayck, Scott K Ferguson, Kathryn Hassell, Rachelle Nuss, Kurt Stenmark, Paul Buehler, David C Irwin
It is now well established that both inherited and acquired forms of hemolytic disease can promote pulmonary vascular disease consequent of free hemoglobin (Hb) induced NO scavenging, elevations in reactive oxygen species and lipid peroxidation. It has recently been reported that oxidative stress can activate NFkB through a toll-like receptor 9 (TLR9) mediated pathway; further, TLR9 can be activated by either nuclear or mitochondrial DNA liberated by stress induced cellular trauma. We hypothesis that Hb induced lipid peroxidation and subsequent endothelial cell trauma is linked to TLR9 activation, resulting in IL-6 mediated pulmonary smooth muscle cell proliferation...
2017: PloS One
https://www.readbyqxmd.com/read/28146290/clinical-findings-treatment-and-outcome-in-11-dairy-heifers-with-breakdown-injury-due-to-interosseous-medius-muscle-rupture
#20
Karl Nuss, Jasmin Boppart, Hans Geyer
OBJECTIVE: To describe the diagnosis, treatment, and prognosis of fetlock breakdown due to interosseus medius muscle rupture in cattle. STUDY DESIGN: Retrospective clinical study. ANIMALS: Dairy heifers with unilateral or bilateral interosseus medius muscle rupture (n = 11). METHODS: Breakdown injury due to rupture of the interosseus medius muscle was documented clinically, radiographically, and ultrasonographically...
February 2017: Veterinary Surgery: VS
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