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https://www.readbyqxmd.com/read/29764375/the-aquatic-animals-transcriptome-resource-for-comparative-functional-analysis
#1
Chih-Hung Chou, Hsi-Yuan Huang, Wei-Chih Huang, Sheng-Da Hsu, Chung-Der Hsiao, Chia-Yu Liu, Yu-Hung Chen, Yu-Chen Liu, Wei-Yun Huang, Meng-Lin Lee, Yi-Chang Chen, Hsien-Da Huang
BACKGROUND: Aquatic animals have great economic and ecological importance. Among them, non-model organisms have been studied regarding eco-toxicity, stress biology, and environmental adaptation. Due to recent advances in next-generation sequencing techniques, large amounts of RNA-seq data for aquatic animals are publicly available. However, currently there is no comprehensive resource exist for the analysis, unification, and integration of these datasets. This study utilizes computational approaches to build a new resource of transcriptomic maps for aquatic animals...
May 9, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29755448/low-light-anoxygenic-photosynthesis-and-fe-s-biogeochemistry-in-a-microbial-mat
#2
Sebastian Haas, Dirk de Beer, Judith M Klatt, Artur Fink, Rebecca McCauley Rench, Trinity L Hamilton, Volker Meyer, Brian Kakuk, Jennifer L Macalady
We report extremely low-light-adapted anoxygenic photosynthesis in a thick microbial mat in Magical Blue Hole, Abaco Island, The Bahamas. Sulfur cycling was reduced by iron oxides and organic carbon limitation. The mat grows below the halocline/oxycline at 30 m depth on the walls of the flooded sinkhole. In situ irradiance at the mat surface on a sunny December day was between 0.021 and 0.084 μmol photons m-2 s-1 , and UV light (<400 nm) was the most abundant part of the spectrum followed by green wavelengths (475-530 nm)...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29744470/organoid-culture-of-bladder-cancer-cells
#3
EDITORIAL
Takahiro Yoshida, Alok K Singh, William R Bishai, David J McConkey, Trinity J Bivalacqua
No abstract text is available yet for this article.
May 2018: Investigative and Clinical Urology
https://www.readbyqxmd.com/read/29738562/validation-of-the-quantitative-point-of-care-carestart-biosensor-for-assessment-of-g6pd-activity-in-venous-blood
#4
Germana Bancone, Gornpan Gornsawun, Cindy S Chu, Pen Porn, Sampa Pal, Pooja Bansil, Gonzalo J Domingo, Francois Nosten
INTRODUCTION: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzymopathy in the human population affecting an estimated 8% of the world population, especially those living in areas of past and present malaria endemicity. Decreased G6PD enzymatic activity is associated with drug-induced hemolysis and increased risk of severe neonatal hyperbilirubinemia leading to brain damage. The G6PD gene is on the X chromosome therefore mutations cause enzymatic deficiency in hemizygote males and homozygote females while the majority of heterozygous females have an intermediate activity (between 30-80% of normal) with a large distribution into the range of deficiency and normality...
2018: PloS One
https://www.readbyqxmd.com/read/29720793/edgar-adrian-and-patrick-merton-names-blurred-with-the-passage-of-time
#5
Kalyan B Bhattacharyya
Edgar Douglas Adrian and Patrick Anthony Merton are two supreme neurophysiologists from England in the last century whose names are almost forgotten these days. Adrian's work on all-or-none phenomenon in nerve and muscle excitability ushered in a new era and Merton's servo theory of muscular movement and muscle fatigue added a new dimension to the understanding of stretch reflex and deep tendon reflexes. Both of them trained and worked at Trinity College, Cambridge and both were elected as Fellow of the Royal Society and Adrian in addition, was awarded the Nobel Prize in 1932 along with Charles Scott Sherrington...
January 2018: Annals of Indian Academy of Neurology
https://www.readbyqxmd.com/read/29716401/clinical-accuracy-of-a-patient-specific-femoral-osteotomy-guide-in-minimally-invasive-posterior-hip-arthroplasty
#6
Adrian K Schneider, Jim W Pierrepont, Gabrielle Hawdon, Stephen McMahon
INTRODUCTION: Patient specific guides can be a valuable tool in improving the precision of planned femoral neck osteotomies, especially in minimally invasive hip surgery, where bony landmarks are often inaccessible. The aim of our study was to validate the accuracy of a novel patient specific femoral osteotomy guide for THR through a minimally invasive posterior approach, the direct superior approach (DSA). METHODS: As part of our routine preoperative planning 30 patients underwent low dose CT scans of their arthritic hip...
April 1, 2018: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29707046/-de-novo-assembly-of-the-burying-beetle-nicrophorus-orbicollis-coleoptera-silphidae-transcriptome-across-developmental-stages-with-identification-of-key-immune-transcripts
#7
Harim I Won, Thomas T Schulze, Emalie J Clement, Gabrielle F Watson, Sean M Watson, Rosalie C Warner, Elizabeth A M Ramler, Elias J Witte, Mark A Schoenbeck, Claudia M Rauter, Paul H Davis
Burying beetles ( Nicrophorus spp.) are among the relatively few insects that provide parental care while not belonging to the eusocial insects such as ants or bees. This behavior incurs energy costs as evidenced by immune deficits and shorter life-spans in reproducing beetles. In the absence of an assembled transcriptome, relatively little is known concerning the molecular biology of these beetles. This work details the assembly and analysis of the Nicrophorus orbicollis transcriptome at multiple developmental stages...
2018: Journal of Genomics
https://www.readbyqxmd.com/read/29694018/dual-carbon-confined-sno-2-hollow-nanospheres-enabling-high-performance-for-the-reversible-storage-of-alkali-metal-ions
#8
Qiong Wu, Qi Shao, Qiang Li, Qian Duan, Yanhui Li, Heng-Guo Wang
To explore a universal electrode material for the high-performance electrochemical storage of Li+ , Na+ , and K+ ions remains a big challenge. Herein, we propose a "trinity" strategy to coat the SnO2 hollow nanospheres using the dual carbon layer from the polydopamine-derived nitrogen-doped carbon and graphene. Thereinto, hollow structures with sufficient void space could buffer the volume expansion, whereas dual carbon-confined strategy could not only elastically prevent the aggregation of nanoparticle and ensure the structural integrity but also immensely improve the conductivity and endow high rate properties...
April 25, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29689178/gender-differences-in-psychosocial-and-physical-outcomes-in-haitian-amputees
#9
Pey-Shan Wen, Marilys G Randolph, Leonard Elbaum, Mario De la Rosa
OBJECTIVE: The purpose of this study was to investigate the gender differences in psychosocial and physical outcomes in users of lower-extremity prostheses who became amputees after the 2010 earthquake in Haiti. METHOD: We enrolled 140 unilateral amputees in this cross-sectional study in Port-au-Prince, Haiti. Trained staff administered the assessments by reading the questions aloud to participants. Participants completed the Trinity Amputation and Prosthesis Experience Scales and Locomotor Capabilities Index about 2 yr after the earthquake...
May 2018: American Journal of Occupational Therapy: Official Publication of the American Occupational Therapy Association
https://www.readbyqxmd.com/read/29649957/tissue-engineered-neo-urinary-conduit-from-decellularized-trachea
#10
Anirudha Anirudhasingh Gmail Com Singh, David Lee, Harrison Jeong, Christine Yu, Jiuru Li, ChenHao Fang, Praveena Sabnekar, Xiaopu Liu, Takahiro Yoshida, Nikolai Sopko, Trinity Bivalacqua
Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea as well as other tubular organs. A neo-urinary conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed...
April 13, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29627969/learning-approaches-among-the-pre-clinical-medical-students-at-trinity-school-of-medicine-st-vincent-and-the-grenadines-and-its-academic-significance
#11
Keshab Raj Paudel, Hari Prasad Nepal, Binu Shrestha, Raju Panta, Stephen Toth
Purpose: Different students may adopt different learning approaches, namely deep and surface. This study was aimed to find out the learning strategies of medical students at Trinity School of Medicine and observe a correlation between deep learning approach and academic scores of the students. Methods: The study was a questionnaire-based cross-sectional observational study. One hundred sixty nine medical students in the basic science years of training were included in the study after giving informed consent...
April 6, 2018: Journal of Educational Evaluation for Health Professions
https://www.readbyqxmd.com/read/29626415/effects-of-common-hemoglobin-variants-on-hba1c-measurements-in-china-results-for-%C3%AE-and-%C3%AE-globin-variants-measured-by-six-methods
#12
Anping Xu, Weidong Chen, Yong Xia, Yu Zhou, Ling Ji
BACKGROUND: HbA1c is a widely used biomarker for diabetes mellitus management. Here, we evaluated the accuracy of six methods for determining HbA1c values in Chinese patients with common α- and β-globin chains variants in China. METHODS: Blood samples from normal subjects and individuals exhibiting hemoglobin variants were analyzed for HbA1c, using Sebia Capillarys 2 Flex Piercing (C2FP), Bio-Rad Variant II Turbo 2.0, Tosoh HLC-723 G8 (ver. 5.24), Arkray ADAMS A1c HA-8180V fast mode, Cobas c501 and Trinity Ultra2 systems...
April 7, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29621308/acceptability-and-performance-of-a-directly-assisted-oral-hiv-self-testing-intervention-in-adolescents-in-rural-mozambique
#13
Jonas Hector, Mary-Ann Davies, Johanna Dekker-Boersema, Mussa Manuel Aly, Cassimo Charifo A Abdalad, Ernesto Belario Rafael Langa, Jochen Ehmer, Michael Andre Hobbins, Laura Frances Jefferys
INTRODUCTION: Whereas progress in HIV testing and treatment has been made globally, the UNAIDS goal of "90 90 90" is still out of sight in rural northern Mozambique. New strategies that promote testing in hard to reach groups will aid Mozambique's response to the HIV epidemic. HIV self-testing (HIVST) is recommended by the WHO as an additional approach to augment the HIV testing services available to adolescents. This study evaluates acceptability and performance of a directly assisted oral HIVST intervention for adolescents in rural Mozambique...
2018: PloS One
https://www.readbyqxmd.com/read/29617714/transcriptome-profiling-of-genes-involving-in-carotenoid-biosynthesis-and-accumulation-between-leaf-and-root-of-carrot-daucus-carota-l
#14
Jing Ma, Jingwen Li, Zhisheng Xu, Feng Wang, Aisheng Xiong
Carrot provides abundant carotenoid for human diet and is one of the most widely cultivated root vegetables in the world. However, the absence of the tissue-specific transcriptome of carrots hampers the investigation of the association of secondary metabolic mechanism with the different tissue types. In this study, we obtained 46,119,008/48,414,508 raw reads and 45,394,846/47,887,648 clean reads from the carrot leaf and root, respectively. Moreover, α- and β-carotene were found to accumulate in both tissues...
March 29, 2018: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29605602/de-novo-transcriptome-sequencing-and-comprehensive-analysis-of-the-heat-stress-response-genes-in-the-basidiomycetes-fungus-ganoderma-lucidum
#15
Xiaoyan Tan, Junshe Sun, Huijuan Ning, Zifang Qin, Yuxin Miao, Tian Sun, Xiuqing Zhang
Ganoderma lucidum is a valuable basidiomycete with numerous pharmacological compounds, which is widely consumed throughout China. We previously found that the polysaccharide content of Ganoderma lucidum fruiting bodies could be significantly improved by 45.63% with treatment of 42 °C heat stress (HS) for 2 h. To further investigate genes involved in HS response and explore the mechanisms of HS regulating the carbohydrate metabolism in Ganoderma lucidum, high-throughput RNA-Seq was conducted to analyse the difference between control and heat-treated mycelia at transcriptome level...
March 29, 2018: Gene
https://www.readbyqxmd.com/read/29604462/physiological-and-transcriptomic-analysis-revealed-the-involvement-of-crucial-factors-in-heat-stress-response-of-rhododendron-hainanense
#16
Ying Zhao, Wengang Yu, Xiangyu Hu, Youhai Shi, Yu Liu, Yunfang Zhong, Peng Wang, Shuya Deng, Jun Niu, Xudong Yu
Molecular regulatory mechanism of heat stress response (HSR) in Ericaceae remains unknown. Here, we sought to identify HSR mechanisms in Rhododendron hainanense, a Ericaceae species, through a combination of physiological and transcriptomic studies. The levels of MDA, H2 O2 , Pro, SOD, CAT and APX in leaves of R. hainanense were analyzed to characterize a dramatic difference in varied temperature treatment. Also, three sequencing libraries, including one control and two heat stress (HS)-treated samples, were constructed for comparative transcriptomic analysis...
March 28, 2018: Gene
https://www.readbyqxmd.com/read/29604234/increased-skeletal-muscle-mitochondrial-free-radical-production-in-peripheral-arterial-disease-despite-preserved-mitochondrial-respiratory-capacity
#17
Corey R Hart, Gwenael Layec, Joel D Trinity, Oh Sung Kwon, Jia Zhao, Van R Reese, Jayson R Gifford, Russell S Richardson
Skeletal muscle mitochondrial dysfunction, which is not fully explained by disease-related arterial occlusion, has been implicated in the pathophysiology of peripheral arterial disease (PAD). Therefore, this study comprehensively assessed mitochondrial respiratory function in biopsies from the gastrocnemius of 10 patients with PAD (Fontaine Stage II) and 12 healthy controls (HC). Intramuscular and systemic inflammation, mitochondrial-derived reactive oxygen species (ROS) production, and oxidative stress were also assessed to better understand the mechanisms responsible for the proposed PAD-induced mitochondrial dysfunction...
March 31, 2018: Experimental Physiology
https://www.readbyqxmd.com/read/29590512/progress-its-glories-and-pitfalls
#18
Daniel Callahan
Steven Pinker, a cognitive psychologist and linguist at Harvard and a savant of big ideas, is one of the latest to take on the idea of progress. He does it under the aegis of "enlightenment," which comes down to a kind of holy trinity of reason, science, and humanism. His new book, Enlightenment Now: The Case for Reason, Science, Humanism, and Progress, is ambitious and cantankerous and heady with hope. On the whole, Pinker makes a good case for the benefits of progress, but with an overdose of feel-good prose...
March 2018: Hastings Center Report
https://www.readbyqxmd.com/read/29584857/impaired-muscle-efficiency-but-preserved-peripheral-hemodynamics-and-mitochondrial-function-with-advancing-age-evidence-from-exercise-in-the-young-old-and-oldest-old
#19
Gwenael Layec, Joel D Trinity, Corey R Hart, Yann Le Fur, Jia Zhao, Van Reese, Eun-Kee Jeong, Russell S Richardson
Muscle weakness in the elderly has been linked to recurrent falls and morbidity, therefore, elucidating the mechanisms contributing to the loss of muscle function and mobility with advancing age is critical. To this aim, we comprehensively examined skeletal muscle metabolic function and hemodynamics in 11 young (23±2 yrs), 11 old (68±2 yrs), and 10 oldest-old (84±2 yrs) physical activity-matched subjects. Specifically, oxidative stress markers, mitochondrial function and the ATP cost of contraction as well as peripheral hemodynamics were assessed during dynamic plantar flexion exercise at 40% of maximal work rate (WRmax)...
March 23, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29581927/differential-gene-expression-profiling-through-transcriptome-approach-of-saccharum-spontaneum-l-under-low-temperature-stress-reveals-genes-potentially-involved-in-cold-acclimation
#20
Dharshini Selvarajan, Chakravarthi Mohan, Vignesh Dhandapani, Gauri Nerkar, Ashwin Narayan Jayanarayanan, Manoj Vadakkancherry Mohanan, Naveenarani Murugan, Lovejot Kaur, Mahadevaiah Chennappa, Ravinder Kumar, Minturam Meena, Bakshi Ram, Appunu Chinnaswamy
Sugarcane ( Saccharum sp.) is predominantly grown in both tropics and subtropics in India, and the subtropics alone contribute more than half of sugarcane production. Sugarcane active growth period in subtropics is restricted to 8-9 months mainly due to winter's low temperature stress prevailing during November to February every year. Being a commercial crop, tolerance to low temperature is important in sugarcane improvement programs. Development of cold tolerant sugarcane varieties require a deep knowledge on molecular mechanism naturally adapted by cold tolerant genotypes during low temperature stress...
April 2018: 3 Biotech
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