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Syk 348

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https://www.readbyqxmd.com/read/25612940/phosphorylation-of-spleen-tyrosine-kinase-at-tyrosine-348-psyk%C3%A2-%C3%A2-%C3%A2-may-be-a-marker-of-advanced-phase-of-chronic-myeloid-leukemia-cml
#1
COMPARATIVE STUDY
Céline Bourgne, Alexandre Janel, Juliette Berger, Chantal Rapatel, Olivier Tournilhac, Eric Hermet, Agnès Guerci, Frédérique Lioret, Aurélie Briançon, Mahchid Bamdad, Nathalie Boiret-Dupré, Marc G Berger
We investigated Syk as a potential marker of CML progression. We observed a significant over-expression of Syk mRNA and constitutive phosphorylation of Syk Y348 in blast cells from six AP or BP-CML, but not in 15 CML in chronic phase. We could follow in vivo the recurrence of pSyk(348) throughout blast cell escape, despite observing storage of dasatinib in blast cells. A combination of dasatinib and R406 did not improve therapeutic efficacy in vitro. Our results strongly suggest that Syk activation could be a relevant biomarker of disease progression and dasatinib resistance but is probably not a molecular target...
March 2015: Leukemia Research
https://www.readbyqxmd.com/read/21334982/treatment-with-tnf%C3%AE-blockers-induces-phenotypical-and-functional-aberrations-in-peripheral-b-cells
#2
Maria P Karampetsou, Andrew P Andonopoulos, Stamatis-Nick C Liossis
To dissect the mechanisms of anti-TNFα-induced autoimmunity we examined the phenotype and function of B cells from anti-TNFα-treated patients. Levels of Lyn, Syk, SHP-1, tyrosine 348 phospho-Syk (Y348-Syk) and tyrosine phosphorylated (P-Y) proteins were evaluated and B-cell-surface CD20, CD21 and CD5 were also assessed in 29 patients treated with TNF-α blockers. Following treatment, Lyn, but not Syk or SHP-1, significantly increased particularly in patients with spondyloarthropathies. Increased Lyn levels following treatment correlated with increased Lyn activity as evidenced by a 2...
July 2011: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/16882714/the-vav-binding-site-of-the-non-receptor-tyrosine-kinase-syk-at-tyr-348-is-critical-for-beta2-integrin-cd11-cd18-mediated-neutrophil-migration
#3
COMPARATIVE STUDY
Jurgen Schymeinsky, Anca Sindrilaru, David Frommhold, Markus Sperandio, Ronald Gerstl, Cornelia Then, Attila Mócsai, Karin Scharffetter-Kochanek, Barbara Walzog
Leukocyte adhesion via beta(2) integrins (CD11/CD18) activates the tyrosine kinase Syk. We found that Syk was enriched at the lamellipodium during N-formyl-Met-Leu-Phe-induced migration of neutrophil-like differentiated HL-60 cells. Here, Syk colocalized with Vav, a guanine nucleotide exchange factor for Rac and Cdc42. The enrichment of Syk at the lamellipodium and its colocalization with Vav were absent upon expression of a Syk kinase-dead mutant (Syk K402R) or a Syk mutant lacking the binding site of Vav (Syk Y348F)...
December 1, 2006: Blood
https://www.readbyqxmd.com/read/12717427/hypermethylation-of-the-spleen-tyrosine-kinase-promoter-in-t-lineage-acute-lymphoblastic-leukemia
#4
Patricia A Goodman, Nicole Burkhardt, Brian Juran, Heather E Tibbles, Faith M Uckun
Sequence analysis of the noncoding first exon (exon 1) of the Syk gene demonstrated the presence of a previously cloned CpG island (GenBank #Z 65706). Transient transfection analysis in Daudi cells demonstrated promoter activity (18-fold increase over parental luciferase plasmid) for a 348 bp BstXI-BsrBI fragment containing this island. This region exhibits a high GC content (approximately 75%), contains several SP1 binding sites and a potential initiator sequence, but lacks a strong TATA consensus. Bisulfite sequencing and methylation-specific PCR (MSP) of this region demonstrated that the Syk promoter CpG island was largely unmethylated in B-lineage leukemia cell lines, control peripheral blood cells, human thymocytes and CD3(+) T lymphocytes...
April 24, 2003: Oncogene
https://www.readbyqxmd.com/read/8657103/phospholipase-c-gamma1-interacts-with-conserved-phosphotyrosyl-residues-in-the-linker-region-of-syk-and-is-a-substrate-for-syk
#5
C L Law, K A Chandran, S P Sidorenko, E A Clark
Antigen receptor ligation on lymphocytes activates protein tyrosine kinases and phospholipase C-gamma (PLC-gamma) isoforms. Glutathione S-transferase fusion proteins containing the C-terminal Src-homology 2 [SH2(C)] domain of PLC-gamma1 bound to tyrosyl phosphorylated Syk. Syk isolated from antigen receptor-activated B cells phosphorylated PLC-gamma1 on Tyr-771 and the key regulatory residue Tyr-783 in vitro, whereas Lyn from the same B cells phosphorylated PLC-gamma1 only on Tyr-771. The ability of Syk to phosphorylate PLC-gamma1 required antigen receptor ligation, while Lyn was constitutively active...
April 1996: Molecular and Cellular Biology
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