keyword
https://read.qxmd.com/read/38065896/a-pan-cancer-analysis-of-tnfaip8l1-in-human-tumors
#1
JOURNAL ARTICLE
Jinghui Sun, Xuezhong Zhang, Bin Zhu, Yingjun Chen, Hui Wang
TNFAIP8L1, as a recently identified member in TNFAIP8 family, plays an important role in tumorigenesis. However, a pan-cancer analysis of TNFAIP8L1 in human tumors has not been conducted until now. The main purpose of study is to investigate TNFAIP8L1 during 33 different types of human tumors by using TCGA and GTEx. The pan-cancer analysis showed that TNFAIP8L1 was significantly over-expressed in 15 cancers and low-expressed in 9 cancers. There were distinct relations between TNFAIP8L1 expression and prognosis of patients with cancer...
December 8, 2023: Medicine (Baltimore)
https://read.qxmd.com/read/37078291/bcl6b-contributes-to-ocular-vascular-diseases-via-notch-signal-silencing
#2
JOURNAL ARTICLE
Miruto Tanaka, Shinsuke Nakamura, Tomohisa Sakaue, Takumi Yamamoto, Masashi Maekawa, Anri Nishinaka, Hiroto Yasuda, Kaori Yunoki, Yuji Sato, Masaaki Sawa, Kohichiro Yoshino, Masamitsu Shimazawa, Masahiko Hatano, Takeshi Tokuhisa, Shigeki Higashiyama, Hideaki Hara
BACKGROUND: Endothelial cell activation is tightly controlled by the balance between VEGF (vascular endothelial cell growth factor) and Notch signaling pathway. VEGF destabilizes blood vessels and promotes neovascularization, which are common features of sight-threatening ocular vascular disorders. Here, we show that BCL6B (B-cell CLL/lymphoma 6 member B protein), also known as BAZF, ZBTB28, and ZNF62, plays a pivotal role in the development of retinal edema and neovascularization. METHODS: The pathophysiological physiological role of BCL6B was investigated in cellular and animal models mimicking 2 pathological conditions: retinal vein occlusion and choroidal neovascularization...
June 2023: Arteriosclerosis, Thrombosis, and Vascular Biology
https://read.qxmd.com/read/36963391/allogeneic-offspring-produced-by-induction-of-pd-l1-in-spermatogonial-stem-cells-via-self-renewal-stimulation
#3
JOURNAL ARTICLE
Takashi Shinohara, Takuya Yamamoto, Hiroko Morimoto, Yusuke Shiromoto, Mito Kanatsu-Shinohara
The testis is an immune-privileged organ. It is considered that the testis somatic microenvironment is responsible for immune suppression. However, immunological properties of spermatogonial stem cells (SSCs) have remained unknown. Here, we report the birth of allogeneic offspring by enhanced expression of immunosuppressive PD-L1 in SSCs. In vitro supplementation of GDNF and FGF2 increased expression of PD-L1 in SSCs. Cultured SSCs maintained allogeneic spermatogenesis that persisted for >1 year. However, depletion or gene editing of Pd-l1 family genes in SSCs prevented allogeneic spermatogenesis, which suggested that germ cells are responsible for suppression of the allogeneic response...
March 14, 2023: Stem Cell Reports
https://read.qxmd.com/read/36389136/b-cell-lymphoma-6a-regulates-immune-development-and-function-in-zebrafish
#4
JOURNAL ARTICLE
Farooq L J Almohaisen, Somayyeh Heidary, Mohamed L Sobah, Alister C Ward, Clifford Liongue
BCL6A is a transcriptional repressor implicated in the development and survival of B and T lymphoctyes, which is also highly expressed in many non-Hodgkin's lymphomas, such as diffuse large B cell lymphoma and follicular lymphoma. Roles in other cell types, including macrophages and non-hematopoietic cells, have also been suggested but require further investigation. This study sought to identify and characterize zebrafish BCL6A and investigate its role in immune cell development and function, with a focus on early macrophages...
2022: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/36376550/circtff1-promotes-proliferation-migration-and-invasion-of-lung-cancer-cells-by-facilitating-methylation-of-bcl6b-promoter-via-mir-29c-3p-dnmt3a-axis
#5
JOURNAL ARTICLE
Lantao Zhao, Hongya Xie, Peng Li, Haisheng Chen, Jiang He, Liang Wang, Yonghong Wang, Bin Ni
Lung cancer exists as a major risk of cancer-related death worldwide. As a novel circular RNA (circRNA), circTFF1 has not been explored in lung cancer thoroughly. This study aims to probe into the function and mechanism of circTFF1 in lung cancer. RT-qPCR and western blot were used to detect expression of RNAs and proteins. The influence of circTFF1 and BCL6B on cells behavior was explored via CCK-8 assay, colony formation assay, wound healing assay and transwell assay. Methylation of BCL6B was detected via MSP assay...
November 14, 2022: Molecular Biotechnology
https://read.qxmd.com/read/35818250/significance-of-screening-sensitive-methylation-sites-using-whole-genome-sequencing-in-early-diagnosis-of-non-small-cell-lung-cancer
#6
JOURNAL ARTICLE
Yu Zhang, Ning Lu, Shunchang Pu, Kui Mu
The current study aimed to screen the sensitive methylation sites of non-small cell lung cancer by whole-genome sequencing and construct an early warning system for lung cancer. For this purpose, from June 2017 to December 2020, fresh NSCLC tissues and paired adjacent NSCLC tissues from 45 patients were collected. DNA and total RNA were extracted from non-small cell lung cancer (NSCLC) and paired non-cancerous lung tissues. The DNA library combined with a biotinylated probe was collected by Dynabeads m270 streptavidin beads...
February 4, 2022: Cellular and Molecular Biology
https://read.qxmd.com/read/35504087/n6-methyladenosine-rna-demethylase-alkbh5-is-testis-specifically-downregulated-in-hybrid-male-sterile-dzo-and-is-a-target-gene-of-bta-mir-200a
#7
JOURNAL ARTICLE
Huiyou Chen, Jianmin Zhang, Yi Yan, Changhong Zhu, Ling Wang, Shubing Fu, Fuyuan Zuo, Gong-Wei Zhang
N6-methyladenosine (m6 A) is the most common RNA methylation modification in mammals, which is controlled in the male germline to ensure coordinated gene expression in the entire process of spermatogenesis. Dzo is the male offspring of a cross between the domestic cattle (Bos taurus) and yak (Bos grunniens), and is sterile. This study aimed to investigate whether m6 A-associated genes are linked with dzo male sterility. The mRNA expression pattern of m6 A-associated genes and spermatogenesis-related genes modified by m6 A was characterized in cattle, yak, and dzo...
July 15, 2022: Theriogenology
https://read.qxmd.com/read/35470414/regulation-of-spermatogonial-stem-cell-self-renewal-and-proliferation-in-mammals
#8
REVIEW
Bang-Hong Wei, Shuang-Li Hao, Wan-Xi Yang
The generation of functional sperm relies on spermatogonial stem cells (SSCs) as they can maintain a stem cell pool for continuous generation of functional spermatozoa. The maintenance of SSCs is regulated by several factors. In this paper, we summarize the niche and intrinsic factors in regulating SSC self-renewal and proliferation. GDNF regulates SSC self-renewal through Ras-ERK1/2, SFC, PI3K/Akt and MEK/ERK-mTOR signaling pathways. FGF activates MAPK2K1, ERK and Akt pathways and EGF activates ERK and Akt pathways to induce SSC proliferation...
April 26, 2022: Histology and Histopathology
https://read.qxmd.com/read/35119381/desert-hedgehog-mediates-the-proliferation-of-medaka-spermatogonia-through-smoothened-signaling
#9
JOURNAL ARTICLE
Changle Zhao, Zeming Zhang, Ximei Qu, Xiaoming Bai, Xingyong Liu, Wenjing Tao, Linyan Zhou, Deshou Wang, Jing Wei
Desert hedgehog (DHH) signaling has been reported to be involved in spermatogenesis and the self-renewal of spermatogonial stem cells (SSCs). However, the role of DHH in proliferation of spermatogonia remain to be elucidated. Here we report that Dhh from medaka (Oryizas latipes) (named as OlDhh) could directly mediate the proliferation of spermatogonia via Smoothened (Smo) signaling. Oldhh is 1362 bp in length and encodes 453 amino acid (aa) residues with more than 50% identity with the homologs in other species...
February 1, 2022: Reproduction
https://read.qxmd.com/read/34641993/use-of-alginate-hydrogel-to-improve-long-term-3d-culture-of-spermatogonial-stem-cells-stemness-gene-expression-and-structural-features
#10
JOURNAL ARTICLE
Masoud Hemadi, Vahideh Assadollahi, Ghasem Saki, Afshin Pirnia, Masoud Alasvand, Abolfazl Zendehdel, Mohammadreza Gholami
The quality and quantity of a spermatogonial stem-cell (SSC) culture can be measured in less time using a 3D culture in a scaffold. The present study investigated stemness gene expression and the morphological and structural characterization of SSCs encapsulated in alginate. SSCs were harvested from BALB/c neonatal mice testes through two-step mechanical and enzymatic digestion. The spermatogonial populations were separated using magnetic-activated cell sorting (MACS) using an anti-Thy1 antibody and c-Kit. The SSCs then were encapsulated in alginate hydrogel...
June 2022: Zygote: the Biology of Gametes and Early Embryos
https://read.qxmd.com/read/34269294/bcl6b-hypermethylation-predicts-metastasis-and-poor-prognosis-in-early-stage-hepatocellular-carcinoma-after-thermal-ablation
#11
JOURNAL ARTICLE
Xin Li, Mingzhou Guo, Lili Yang, Zhigang Cheng, Xiaoling Yu, Zhiyu Han, Fangyi Liu, Qiang Sun, Xianjing Han, Jie Yu, Ping Liang
Aims: The aim of this study was to evaluate the role of BCL6B methylation in the progression of early-stage hepatocellular carcinoma (HCC) after thermal ablation. Settings and Design: This is a retrospective study and written informed consent was obtained from all patients or their legal guardians. Subjects and Methods: Between October 2008 and December 2013, 73 patients with early-stage HCC within the Milan criteria, who received thermal ablation, were recruited...
July 2021: Journal of Cancer Research and Therapeutics
https://read.qxmd.com/read/34015032/constitutive-activation-of-ctnnb1-results-in-a-loss-of-spermatogonial-stem-cell-activity-in-mice
#12
JOURNAL ARTICLE
Alexandre Boyer, Xiangfan Zhang, Adrien Levasseur, Nour Abou Nader, Guillaume St-Jean, Makoto C Nagano, Derek Boerboom
Spermatogenesis requires that a careful balance be maintained between the self-renewal of spermatogonial stem cells (SSCs) and their commitment to the developmental pathway through which they will differentiate into spermatozoa. Recently, a series of studies employing various in vivo and in vitro models have suggested a role of the wingless-related MMTV integration site gene family/beta-catenin (WNT/CTNNB1) pathway in determining the fate of SSCs. However, conflicting data have suggested that CTNNB1 signaling may either promote SSC self-renewal or differentiation...
2021: PloS One
https://read.qxmd.com/read/33936161/-klf4-a-key-regulator-of-a-transitive-triplet-acts-on-the-tgf-%C3%AE-signaling-pathway-and-contributes-to-high-altitude-adaptation-of-tibetan-pigs
#13
JOURNAL ARTICLE
Tao Wang, Yuanyuan Guo, Shengwei Liu, Chaoxin Zhang, Tongyan Cui, Kun Ding, Peng Wang, Xibiao Wang, Zhipeng Wang
Tibetan pigs are native mammalian species on the Tibetan Plateau that have evolved distinct physiological traits that allow them to tolerate high-altitude hypoxic environments. However, the genetic mechanism underlying this adaptation remains elusive. Here, based on multitissue transcriptional data from high-altitude Tibetan pigs and low-altitude Rongchang pigs, we performed a weighted correlation network analysis (WGCNA) and identified key modules related to these tissues. Complex network analysis and bioinformatics analysis were integrated to identify key genes and three-node network motifs...
2021: Frontiers in Genetics
https://read.qxmd.com/read/33746576/luteal-phase-ovarian-stimulation-versus-follicular-phase-ovarian-stimulation-results-in-different-human-cumulus-cell-genes-expression-a-pilot-study
#14
JOURNAL ARTICLE
Yu-Chen Chen, Ju-Yueh Li, Chia-Jung Li, Kuan-Hao Tsui, Peng-Hui Wang, Zhi-Hong Wen, Li-Te Lin
Background: Luteal-phase ovarian stimulation (LPOS) is an alternative in vitro fertilization (IVF) protocol. However, limited data showed the genes expression of cumulus cells (CCs) in LPOS. Therefore, this study aimed to investigate CC genes expression between LPOS and follicular-phase ovarian stimulation (FPOS) in poor ovarian responders (PORs) undergoing IVF cycles. Methods: This was a prospective non-randomized trial (ClinicalTrials.gov Identifier: NCT03238833). A total of 36 PORs who met the Bologna criteria and underwent IVF cycles were enrolled...
2021: International Journal of Medical Sciences
https://read.qxmd.com/read/33446567/an-interplay-of-nox1-derived-ros-and-oxygen-determines-the-spermatogonial-stem-cell-self-renewal-efficiency-under-hypoxia
#15
JOURNAL ARTICLE
Hiroko Morimoto, Takuya Yamamoto, Takehiro Miyazaki, Narumi Ogonuki, Atsuo Ogura, Takashi Tanaka, Mito Kanatsu-Shinohara, Chihiro Yabe-Nishimura, Hongliang Zhang, Yves Pommier, Andreas Trumpp, Takashi Shinohara
Reactive oxygen species (ROS) produced by NADPH1 oxidase 1 (NOX1) are thought to drive spermatogonial stem cell (SSC) self-renewal through feed-forward production of ROS by the ROS-BCL6B-NOX1 pathway. Here we report the critical role of oxygen on ROS-induced self-renewal. Cultured SSCs proliferated poorly and lacked BCL6B expression under hypoxia despite increase in mitochondria-derived ROS. Due to lack of ROS amplification under hypoxia, NOX1-derived ROS were significantly reduced, and Nox1 -deficient SSCs proliferated poorly under hypoxia but normally under normoxia...
January 14, 2021: Genes & Development
https://read.qxmd.com/read/33107118/fgf9-promotes-mouse-spermatogonial-stem-cell-proliferation-mediated-by-p38-mapk-signalling
#16
JOURNAL ARTICLE
Fan Yang, Eoin C Whelan, Xuebing Guan, Bingquan Deng, Shu Wang, Jiachen Sun, Mary R Avarbock, Xin Wu, Ralph L Brinster
OBJECTIVES: Fibroblast growth factor 9 (FGF9) is expressed by somatic cells in the seminiferous tubules, yet little information exists about its role in regulating spermatogonial stem cells (SSCs). MATERIALS AND METHODS: Fgf9 overexpression lentivirus was injected into mouse testes, and PLZF immunostaining was performed to investigate the effect of FGF9 on spermatogonia in vivo. Effect of FGF9 on SSCs was detected by transplanting cultured germ cells into tubules of testes...
October 26, 2020: Cell Proliferation
https://read.qxmd.com/read/32771442/short-time-exposure-to-low-concentration-of-zinc-oxide-nanoparticles-up-regulates-self-renewal-and-spermatogenesis-related-gene-expression
#17
JOURNAL ARTICLE
Azam Javadi, Saadat Mokhtari, Seyedeh-Faezeh Moraveji, Forough-Azam Sayahpour, Maryam Farzaneh, Hamid Gourabi, Fereshteh Esfandiari
Extensive application of zinc oxide (ZnO) nanoparticles (NPs) in everyday life results in increased exposure to these NPs. Spermatogonial stem cells (SSCs) guarantee sperm production throughout the male reproductive life by providing a balance between self-renewal and differentiation. We used an in vitro platform to investigate the ZnO NPs effects on SSCs. We successfully synthesized ZnO NPs. In order to investigate these NPs, we isolated SSCs from mouse testes and cultured them in vitro. Our results confirmed the uptake of ZnO NPs by the cultured SSCs...
August 6, 2020: International Journal of Biochemistry & Cell Biology
https://read.qxmd.com/read/32216643/effect-of-equilibration-time-and-temperature-on-murine-spermatogonial-stem-cell-cryopreservation
#18
JOURNAL ARTICLE
Sang-Eun Jung, Myongzun Kim, Jin Seop Ahn, Yong-Hee Kim, Bang-Jin Kim, Min-Hyung Yun, Joong-Hyuck Auh, Buom-Yong Ryu
Cryopreservation of spermatogonial stem cells (SSCs) is essential for preservation of valuable livestock and clinical applications. Although optimal equilibration of cryoprotectants has emerged as a promising approach to improve the cryopreservation efficiency, standard equilibration protocols have not yet been considered in cryopreservation of SSCs. This study aimed to establish a standard equilibration protocol to improve the cryopreservation efficiency of murine germ cells enriched for SSCs. After time- and temperature-dependent equilibration, the germ cells were cryopreserved with 10% dimethyl sulfoxide (DMSO) and 200 mM trehalose...
March 27, 2020: Biopreservation and Biobanking
https://read.qxmd.com/read/32124159/propagation-of-goat-putative-spermatogonial-stem-cells-under-growth-factors-defined-serum-free-culture-conditions
#19
JOURNAL ARTICLE
Ankur Sharma, Syed Mohmad Shah, Manish Tiwari, Mayank Roshan, Manoj Kumar Singh, Suresh Kumar Singla, Prabhat Palta, Radhay Sham Manik, Manmohan Singh Chauhan
In the present study, we used a serum-free culture media to propagate goat putative spermatogonial stem cells (SSCs) and evaluated the effect of crucial growth factors on relative expression of some SSC markers and self-renewal related genes. The enriched SSCs were cultured on a homologous Sertoli cell feeder layer in KO-DMEM supplemented with 10% KOSR. Putative SSC colonies emerged between day 6 and 10 which were then characterized by the expression of numerous spermatogonial and pluripotency related markers...
March 2, 2020: Cytotechnology
https://read.qxmd.com/read/31754389/tumor-suppressive-btb-poz-zinc-finger-protein-zbtb28-inhibits-oncogenic-bcl6-zbtb27-signaling-to-maintain-p53-transcription-in-multiple-carcinogenesis
#20
JOURNAL ARTICLE
Tingxiu Xiang, Jun Tang, Lili Li, Weiyan Peng, Zhenfang Du, Xiangyu Wang, Qianqian Li, Hongying Xu, Lei Xiong, Can Xu, Xin Le, Xufu Wei, Fang Yu, Shuman Li, Qian Xiao, Bing Luo, Xinni Xiang, Ailong Huang, Yong Lin, Guosheng Ren, Qian Tao
Zinc-finger and BTB/POZ domain-containing family proteins (ZBTB) are important transcription factors functioning as tumor suppressors or oncogenes, such as BCL6/ZBTB27 as a key oncoprotein for anti-cancer therapy. Through epigenome study, we identified ZBTB28/BCL6B/BAZF, a BTB/POZ domain protein highly homologous to BCL6, as a methylated target in multiple tumors. However, the functions and mechanism of ZBTB28 in carcinogenesis remain unclear. Methods : ZBTB28 expression and methylation were examined by reverse-transcription PCR and methylation-specific PCR...
2019: Theranostics
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