Chungen Qian, Chao Wan, Shunji Li, Yujin Xiao, Huijuan Yuan, Siyu Gao, Liqiang Wu, Mengfan Zhou, Xiaojun Feng, Yiwei Li, Peng Chen, Bi-Feng Liu
There remains an unmet need for a fully integrated microfluidic platform that can automatically perform multistep and multireagent immunoassays. Here, we proposed a novel online dual-active valve-based centrifugal microfluidic chip, termed DAVM, for fully automatic point-of-care immunoassay. Practically, the puncture valve, one of the dual active valves, is capable of achieving precise, on-demand, sequential release of prestored reagents, while the other valve-reversible active valve enables controlled retention and drainage of the reaction solutions...
August 9, 2023: Analytical Chemistry