keyword
MENU ▼
Read by QxMD icon Read
search

opa1

keyword
https://www.readbyqxmd.com/read/29755919/molecular-phylogenetic-species-in-alternaria-pathogens-infecting-pistachio-and-wild-relatives
#1
Hilal Ozkilinc, Unal Sevinc
Many important pathogens of crops worldwide are members of section Alternaria within the genus Alternaria . Representative species in this section such as Alternaria alternata, Alternaria tenuissima , and Alternaria arborescens show high variability, intermediate characters and plasticity in morphological features, which makes species identification difficult. The aim of this study was to characterize Alternaria species associated with pistachio and wild relatives in Turkey using molecular phylogenetics. One hundred isolates of Alternaria spp...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29748634/gene-by-environment-interactions-that-disrupt-mitochondrial-homeostasis-cause-neurodegeneration-in-c-elegans-parkinson-s-models
#2
Hanna Kim, Rylee J Perentis, Guy A Caldwell, Kim A Caldwell
Parkinson's disease (PD) is a complex multifactorial disorder where environmental factors interact with genetic susceptibility. Accumulating evidence suggests that mitochondria have a central role in the progression of neurodegeneration in sporadic and/or genetic forms of PD. We previously reported that exposure to a secondary metabolite from the soil bacterium, Streptomyces venezuelae, results in age- and dose-dependent dopaminergic (DA) neurodegeneration in Caenorhabditis elegans and human SH-SY5Y neurons...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29748581/leptin-increases-mitochondrial-opa1-via-gsk3-mediated-oma1-ubiquitination-to-enhance-therapeutic-effects-of-mesenchymal-stem-cell-transplantation
#3
Fan Yang, Rongrong Wu, Zhi Jiang, Jinghai Chen, Jinliang Nan, Sheng'an Su, Na Zhang, Chen Wang, Jing Zhao, Cheng Ni, Yingchao Wang, Wangxing Hu, Zhiru Zeng, Keyang Zhu, Xianbao Liu, Xinyang Hu, Wei Zhu, Hong Yu, Jinyu Huang, Jian'an Wang
Accumulating evidence revealed that mesenchymal stem cells (MSCs) confer cardioprotection against myocardial infarction (MI). However, the poor survival and engraftment rate of the transplanted cells limited their therapeutic efficacy in the heart. The enhanced leptin production associated with hypoxia preconditioning contributed to the improved MSCs survival. Mitochondrial integrity determines the cellular fate. Thus, we aimed to investigate whether leptin can enhance mitochondrial integrity of human MSCs (hMSCs) to protect against various stress...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29744667/vagus-nerve-stimulation-exerts-cardioprotection-against-myocardial-ischemia-reperfusion-injury-predominantly-through-its-efferent-vagal-fibers
#4
Watthana Nuntaphum, Wanpitak Pongkan, Suwakon Wongjaikam, Savitree Thummasorn, Pongpan Tanajak, Juthamas Khamseekaew, Kannaporn Intachai, Siriporn C Chattipakorn, Nipon Chattipakorn, Krekwit Shinlapawittayatorn
Vagus nerve stimulation (VNS) has been shown to exert cardioprotection against myocardial ischemia/reperfusion (I/R) injury. However, whether the cardioprotection of VNS is mainly due to direct activation through its ipsilateral efferent fibers (motor) rather than indirect effects mediated by the afferent fibers (sensory) have not been clearly understood. We hypothesized that VNS exerts cardioprotection predominantly through its efferent vagal fibers. Thirty swine (30-35 kg) were randomized into five groups: I/R no VNS (I/R), and left mid-cervical VNS with both vagal trunks intact (LC-VNS), with left vagus nerve transection (LtVNX), with right vagus nerve transection (RtVNX) and with atropine pretreatment (Atropine), respectively...
May 9, 2018: Basic Research in Cardiology
https://www.readbyqxmd.com/read/29742430/manipulation-of-mitochondria-dynamics-reveals-separate-roles-for-form-and-function-in-mitochondria-distribution
#5
Tatiana Trevisan, Diana Pendin, Aldo Montagna, Sergio Bova, Anna Maria Ghelli, Andrea Daga
Mitochondria shape is controlled by membrane fusion and fission mediated by mitofusins, Opa1, and Drp1, whereas mitochondrial motility relies on microtubule motors. These processes govern mitochondria subcellular distribution, whose defects are emphasized in neurons because of their polarized structure. We have studied how perturbation of the fusion/fission balance affects mitochondria distribution in Drosophila axons. Knockdown of Marf or Opa1 resulted in progressive loss of distal mitochondria and in a distinct oxidative phosphorylation and membrane potential deficit...
May 8, 2018: Cell Reports
https://www.readbyqxmd.com/read/29735636/potential-protective-mechanism-in-the-cardiac-microvascular-injury
#6
Xiuchuan Li, Juanni Hou, Jin Du, Jian Feng, Yi Yang, Yang Shen, Sha Chen, Juan Feng, Dachun Yang, De Li, Haifeng Pei, Yongjian Yang
Cardiac microvascular injury often occurs in patients with type 2 diabetes mellitus (T2DM) who develop hyperglycemia and hyperlipidemia. However, besides reported contradictory roles in cardiac diseases, the function of TRPV1 (transient receptor potential vanilloid 1) in cardiac microvessels is not well defined. This study was performed to determine the detailed role of TRPV1 in cardiac microvascular endothelial cells (CMECs) in T2DM. T2DM mice were established by multiple injections of low-dose streptozotocin and high-fat feeding...
May 7, 2018: Hypertension
https://www.readbyqxmd.com/read/29701781/mutant-app-and-amyloid-beta-induced-defective-autophagy-mitophagy-mitochondrial-structural-and-functional-changes-and-synaptic-damage-in-hippocampal-neurons-from-alzheimer-s-disease
#7
P Hemachandra Reddy, XiangLin Yin, Maria Manczak, Subodh Kumar, Pradeepkiran Jangampalli Adi, Murali Vijayan, Arubala P Reddy
The purpose of our study was to determine the toxic effects of hippocampal mutant APP and amyloid beta (Aβ) in human mutant APP (mAPP) cDNA transfected with primary mouse hippocampal neurons (HT22). Hippocampal tissues are the best source of studying learning and memory functions in patients with Alzheimer's disease (AD) and healthy controls. However, investigating immortalized hippocampal neurons that express AD proteins provide an excellent opportunity for drug testing. Using quantitative RT-PCR, immunoblotting & immunofluorescence, and transmission electron microscopy, we assessed mRNA and protein levels of synaptic, autophagy, mitophagy, mitochondrial dynamics, biogenesis, dendritic protein MAP2, and assessed mitochondrial number and length in mAPP-HT22 cells that express Swedish/Indiana mutations...
April 25, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29688962/deterioration-in-distortion-product-otoacoustic-emissions-in-auditory-neuropathy-patients-with-distinct-clinical-and-genetic-backgrounds
#8
Kyoko Kitao, Hideki Mutai, Kazunori Namba, Noriko Morimoto, Atsuko Nakano, Yukiko Arimoto, Tomoko Sugiuchi, Sawako Masuda, Yasuhide Okamoto, Noriko Morita, Hirokazu Sakamoto, Tomoko Shintani, Satoshi Fukuda, Kimitaka Kaga, Tatsuo Matsunaga
OBJECTIVES: Auditory neuropathy (AN) is a clinical disorder characterized by the absence of auditory brainstem response and presence of otoacoustic emissions. A gradual loss of otoacoustic emissions has been reported for some cases of AN. Such cases could be diagnosed as cochlear hearing loss and lead to misunderstanding of the pathology when patients first visit clinics after the loss of otoacoustic emissions. The purpose of this study was to investigate the time course of changes in distortion product otoacoustic emissions (DPOAEs) in association with patients' genetic and clinical backgrounds, including the use of hearing aids...
April 23, 2018: Ear and Hearing
https://www.readbyqxmd.com/read/29688805/opa1-anchored-pka-phosphorylates-perilipin-1-on-s522-and-s497-in-adipocytes-differentiated-from-human-adipose-stem-cells
#9
Marie Rogne, Dinh-Toi Chu, Thomas M Küntziger, Maria-Niki Mylonakou, Philippe Collas, Kjetil Tasken
Optic atrophy 1 (OPA1) is the A-kinase anchoring protein (AKAP) targeting the pool of PKA responsible for perilipin 1 phosphorylation, a gatekeeper for lipolysis. However, the involvement of OPA1-bound PKA in the downstream regulation of lipolysis is unknown. Here, we show upregulation and relocation of OPA1 from mitochondria to lipid droplets during adipocytic differentiation of human adipose stem cells (hASCs). We employed various biochemical and immunological approaches to demonstrate that OPA1-bound PKA phosphorylates perilipin 1 at S522 and S497 upon lipolytic stimulation...
April 24, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29687234/ros-as-regulators-of-mitochondrial-dynamics-in-neurons
#10
REVIEW
Carolina Cid-Castro, Diego Rolando Hernández-Espinosa, Julio Morán
Mitochondrial dynamics is a complex process, which involves the fission and fusion of mitochondrial outer and inner membranes. These processes organize the mitochondrial size and morphology, as well as their localization throughout the cells. In the last two decades, it has become a spotlight due to their importance in the pathophysiological processes, particularly in neurological diseases. It is known that Drp1, mitofusin 1 and 2, and Opa1 constitute the core of proteins that coordinate this intricate and dynamic process...
April 23, 2018: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/29672614/adaptation-of-motor-unit-contractile-properties-in-rat-medial-gastrocnemius-to-treadmill-endurance-training-relationship-to-muscle-mitochondrial-biogenesis
#11
Katarzyna Kryściak, Joanna Majerczak, Jakub Kryściak, Dawid Łochyński, Dominik Kaczmarek, Hanna Drzymała-Celichowska, Piotr Krutki, Anna Gawedzka, Magdalena Guzik, Michał Korostynski, Zbigniew Szkutnik, Elżbieta Pyza, Wiesława Jarmuszkiewicz, Jerzy A Zoladz, Jan Celichowski
This study aimed at investigating the effects of 2, 4 and 8 weeks of endurance training on the contractile properties of slow (S), fast fatigue resistant (FR) and fast fatigable (FF) motor units (MUs) in rat medial gastrocnemius (MG) in relation to the changes in muscle mitochondrial biogenesis. The properties of functionally isolated MUs were examined in vivo. Mitochondrial biogenesis was judged based on the changes in mitochondrial DNA copy number (mtDNA), the content of the electron transport chain (ETC) proteins and PGC-1α in the MG...
2018: PloS One
https://www.readbyqxmd.com/read/29604226/stem-cell-modeling-of-mitochondrial-parkinsonism-reveals-key-functions-of-opa1
#12
Mindaugas Jonikas, Martin Madill, Alexandre Mathy, Theresa Zekoll, Christos E Zois, Simon Wigfield, Marzena Kurzawa-Akanbi, Cathy Browne, David Sims, Patrick F Chinnery, Sally A Cowley, George K Tofaris
OBJECTIVE: Defective mitochondrial function due to OPA1 mutations causes primarily optic atrophy and less commonly neurodegenerative syndromes. The pathomechanism by which OPA1 mutations trigger diffuse loss of neurons in some but not all patients is unknown. Here we used a tractable iPSC-based model to capture the biology of OPA1 haploinsufficiency in cases presenting with classic eye disease versus syndromic parkinsonism. METHODS: iPSC were generated from two patients with OPA1 haploinsufficiency and two controls and differentiated into dopaminergic neurons...
March 31, 2018: Annals of Neurology
https://www.readbyqxmd.com/read/29601815/discovery-and-characterization-of-selective-small-molecule-inhibitors-of-the-mammalian-mitochondrial-division-dynamin-drp1
#13
A Mallat, L F Uchiyama, S C Lewis, R A Fredenburg, Y Terada, N Ji, J Nunnari, C C Tseng
Balanced rates of mitochondrial division and fusion are required to maintain mitochondrial function, as well as cellular and organismal homeostasis. In mammals, the cellular machines that mediate these processes are dynamin-related GTPases; the cytosolic DRP1 mediates division, while the outer membrane MFN1/2 and inner membrane OPA1 mediate fusion. Unbalanced mitochondrial dynamics are linked to varied pathologies, including cell death and neurodegeneration, raising the possibility that small molecules that target the division and fusion machines to restore balance may have therapeutic potential...
March 27, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29563951/genetic-testing-for-wolfram-syndrome-mutations-in-a-sample-of-71-patients-with-hereditary-optic-neuropathy-and-negative-genetic-test-results-for-opa1-opa3-lhon
#14
Alberto Galvez-Ruiz, Alicia Galindo-Ferreiro, Patrik Schatz
In this study, the authors present a sample of 71 patients with hereditary optic neuropathy and negative genetic test results for OPA1/OPA3/LHON. All of these patients later underwent genetic testing to rule out WFS. As a result, 53 patients (74.7%) were negative and 18 patients (25.3%) were positive for some type of mutation or variation in the WFS gene. The authors believe that this study is interesting because it shows that a sizeable percentage (25.3%) of patients with hereditary optic 25 neuropathy and negative genetic test results for OPA1/OPA3/LHON had WFS mutations or variants...
April 2018: Neuro-ophthalmology
https://www.readbyqxmd.com/read/29545505/m-aaa-and-i-aaa-complexes-coordinate-to-regulate-oma1-the-stress-activated-supervisor-of-mitochondrial-dynamics
#15
Francesco Consolato, Francesca Maltecca, Susanna Tulli, Irene Sambri, Giorgio Casari
The proteolytic processing of dynamin-like GTPase OPA1, mediated by the activity of both YME1L1 [intermembrane (i)-AAA protease complex] and OMA1, is a crucial step in the regulation of mitochondrial dynamics. OMA1 is a zinc metallopeptidase of the inner mitochondrial membrane that undergoes pre-activating proteolytic and auto-proteolytic cleavage after mitochondrial import. Here, we identify AFG3L2 [matrix (m) - AAA complex] as the major protease mediating this event, which acts by maturing the 60 kDa pre-pro-OMA1 to the 40 kDa pro-OMA1 form by severing the N-terminal portion without recognizing a specific consensus sequence...
April 9, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29527615/intracellular-ca-2-release-decelerates-mitochondrial-cristae-dynamics-within-the-junctions-to-the-endoplasmic-reticulum
#16
Benjamin Gottschalk, Christinae Klec, Markus Waldeck-Weiermair, Roland Malli, Wolfgang F Graier
Mitochondria are multifunctional organelles that essentially contribute to cell signaling by sophisticated mechanisms of communications. Live cell imaging studies showed that mitochondria are dynamic and complex structures that form ramified networks by directed movements, fission, and fusion events. There is emerging evidence that the morphology of mitochondria determines cellular functions and vice versa. Several intracellular signaling pathways and messengers including Ca2+ dynamically influence the architecture of mitochondria...
March 12, 2018: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/29523844/differentiation-by-nerve-growth-factor-ngf-involves-mechanisms-of-crosstalk-between-energy-homeostasis-and-mitochondrial-remodeling
#17
Francesca Martorana, Daniela Gaglio, Maria Rosaria Bianco, Federica Aprea, Assunta Virtuoso, Marcella Bonanomi, Lilia Alberghina, Michele Papa, Anna Maria Colangelo
Neuronal differentiation involves extensive modification of biochemical and morphological properties to meet novel functional requirements. Reorganization of the mitochondrial network to match the higher energy demand plays a pivotal role in this process. Mechanisms of neuronal differentiation in response to nerve growth factor (NGF) have been largely characterized in terms of signaling, however, little is known about its impact on mitochondrial remodeling and metabolic function. In this work, we show that NGF-induced differentiation requires the activation of autophagy mediated by Atg9b and Ambra1, as it is disrupted by their genetic knockdown and by autophagy blockers...
March 9, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29519277/-effects-of-heme-oxygenase-1-carbon-monoxide-pathway-on-the-mitochondrial-fusion-in-rat-alveolar-epithelial-type-ii-cells-stimulated-by-lipopolysaccharide
#18
Haojuan Jia, Jia Shi, Shu'an Dong, Yuan Zhang, Jianbo Yu
OBJECTIVE: To investigate the effects of heme oxygenase-1/carbon monoxide (HO-1/CO) pathway on mitochondrial fusion in rat alveolar epithelial type II cells (AEC II) stimulated by lipopolysaccharide (LPS). METHODS: Once the cultured in vitro rat AEC II cells line RLE-6TN reached confluency of 85%, they were subcultured and randomly divided into seven groups (n = 5 each). RLE-6TN cells were routinely cultured in control group. The cells in LPS group was stimulated with 10 mg/L LPS to reproduce the model of endotoxin challenge in AECII cells...
March 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29505116/a-new-high-resolution-melting-assay-for-genotyping-alternaria-species-causing-citrus-brown-spot
#19
Francesca Garganese, Antonio Ippolito, Valentina di Rienzo, Concetta Lotti, Cinzia Montemurro, Simona M Sanzani
BACKGROUND: Alternaria brown spot is one of the most important diseases of tangerines and their hybrids worldwide. To set up effective control strategy, the accurate detection and identification of the species responsible for the diseases is crucial. However, characterization based on morphology and/or multilocus genetic approaches is time consuming, requires great expertise and sometimes is not conclusive. Therefore, the set-up of a rapid and efficient DNA-based assay might be of paramount importance...
March 5, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29503614/increased-degradation-rates-in-the-components-of-the-mitochondrial-oxidative-phosphorylation-chain-in-the-cerebellum-of-old-mice
#20
Aurel Popa-Wagner, Raluca E Sandu, Coman Cristin, Adriana Uzoni, Kevin A Welle, Jennifer R Hryhorenko, Sina Ghaemmaghami
Brain structures differ in the magnitude of age-related neuron loss with the cerebellum being more affected. An underlying cause could be an age-related decline in mitochondrial bioenergetics. Successful aging of mitochondria reflects a balanced turnover of proteins involved in mitochondrial biogenesis and mitophagy. Thus, an imbalance in mitochondrial turnover can contribute to the diminution of cellular function seen during aging. Mitochondrial biogenesis and mitophagy are mediated by a set of proteins including MFN1, MFN2, OPA1, DRP1, FIS1 as well as DMN1l and DNM1, all of which are required for mitochondrial fission...
2018: Frontiers in Aging Neuroscience
keyword
keyword
42250
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"