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https://www.readbyqxmd.com/read/29909468/a-high-incidence-and-coexistence-of-multiresistance-genes-cfr-and-optra-among-linezolid-resistant-enterococci-isolated-from-a-teaching-hospital-in-wenzhou-china
#1
Yizhi Zhang, Guofeng Dong, Jiahui Li, Lijiang Chen, Haiyang Liu, Wenzi Bi, Hong Lu, Tieli Zhou
Linezolid is considered as a last-resort antimicrobial agent, the resistance of which is of great concern. The aim of this study was to investigate the mechanisms and transferability of linezolid resistance and molecular epidemiology of linezolid-resistant enterococcal isolates in Wenzhou, China. A collection of 1623 enterococcal strains, including 789 Enterococcus faecalis and 834 Enterococcus faecium, were isolated from our hospital during 2011-2016. Antimicrobial susceptibility testing and clinical data analysis were performed...
June 16, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29909242/initial-characterization-of-newly-identified-mitochondrial-and-chloroplast-small-hsps-from-sugarcane-shows-that-these-chaperones-have-different-oligomerization-states-and-substrate-specificities
#2
Glaucia M S Pinheiro, Carlos H I Ramos
Chaperones belonging to the small heat shock protein (sHSP) family are ubiquitous and exhibit elevated expression under stresses conditions to protect proteins against aggregation, thereby contributing to the stress tolerance of the organism. Tropical plants are constantly exposed to high temperatures, and the mechanisms by which these plants tolerate heat stress are of foremost importance to basic science as well as applied agrobiotechnology. Therefore, this study aims to characterize sHSPs from different organelles from sugarcane, an important crop that is associated with sugar and bioenergy production...
June 4, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29909068/effects-of-histone-methyltransferase-inhibitor-chaetocin-on-histone-h3k9-methylation-of-cultured-ovine-somatic-cells-and-development-of-preimplantation-cloned-embryos
#3
Yu-Mei Zhang, En-En Gao, Qian-Qian Wang, Hao Tian, Jian Hou
Aberrant hypermethylation of histone H3 lysine 9 (H3K9) is a key barrier to the development of cloned embryos by somatic cell nuclear transfer (SCNT). The objective of this study was to assess the effects of chaetocin, an inhibitor of H3K9 methyltransferase SUV39 H, in regulating the H3K9 methylation in ovine SCNT embryos. Treatment of sheep fetal fibroblast cells with chaetocin specifically decreased the levels of H3K9 di-and trimethylation, and down-regulated the expression of H3K9 methyltransferases, SUV39H1/2 and G9A...
June 14, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29909020/diet-modifies-colonic-microbiota-and-cd4-t-cell-repertoire-to-induce-flares-of-colitis-in-mice-with-myeloid-cell-expression-of-interleukin-23
#4
Lili Chen, Zhengxiang He, Alina Cornelia Iuga, Sebastião N Martins Filho, Jeremiah J Faith, Jose C Clemente, Madhura Deshpande, Anitha Jayaprakash, Jean-Frederic Colombel, Juan J Lafaille, Ravi Sachidanandam, Glaucia C Furtado, Sergio A Lira
BACKGROUND & AIMS: Several studies have shown that signaling via the interleukin 23 (IL23) receptor is required for development of colitis. We studied the roles of IL23, dietary factors, alterations to the microbiota, and T cells in development and progression of colitis in mice. METHODS: All mice were maintained on lab diet 5053, unless otherwise noted. We generated mice that express IL23 in CX3CR1-positive myeloid cells (R23FR mice) upon cyclic administration of tamoxifen dissolved in diet 2019...
June 14, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29908428/clastogenecity-evaluation-of-water-of-lake-sevan-armenia-using-tradescantia-micronucleus-assay
#5
E A Aghajanyan, R E Avalyan, A E Simonyan, A L Atoyants, B K Gabrielyan, R M Aroutiounian, A Khosrovyan
The clastogenic effects of water samples in seven locations of Lake Sevan (Armenia) with the application of Trad-MCN (micronuclei) bioassay using Tradescantia (clone 02) were investigated. A significant increase in the frequency of micronuclei in tetrads of pollen microspores and tetrads with micronuclei exposed to the test samples compared to the control has been revealed. A multivariate analysis indicated linkage between the frequencies of occurrence of micronuclei in the cells and Ni and Co ions. The results were compared with the endpoints of another Tradescantia-based test system (stamen hair mutation test) performed on the same water samples and generation of the plant: occurrences of micronuclei in sporogenic cells coincided with that of non-surviving stamen hair...
May 24, 2018: Chemosphere
https://www.readbyqxmd.com/read/29908003/molecular-cloning-of-tamate2-homoeologues-potentially-related-to-aluminium-tolerance-in-bread-wheat-triticum-aestivum-l
#6
Ana Luísa Garcia-Oliveira, César Benito, Henrique Guedes-Pinto, Paula Martins-Lopes
Recently, members of MATE family have been implicated in aluminium (Al) tolerance by facilitating citrate efflux in plants. The aim of the present work was to perform a molecular characterisation of the MATE2 gene in bread wheat. Here, we cloned a member of the MATE gene family in bread wheat and named TaMATE2, which showed the typical secondary structure of MATE-type transporters maintaining all the 12 transmembrane domains. The amplification in Chinese Spring nulli-tetrasomic and ditelosomic lines revealed that TaMATE2 is located on the long arm of homoeologous group 1 chromosomes...
June 15, 2018: Plant Biology
https://www.readbyqxmd.com/read/29907831/strontium-ranelate-promotes-odonto-osteogenic-differentiation-mineralization-of-dental-papillae-cells-in-vitro-and-mineralized-tissue-formation-of-the-dental-pulp-in-vivo
#7
Alamuddin Bakhit, Nobuyuki Kawashima, Kentaro Hashimoto, Sonoko Noda, Keisuke Nara, Masashi Kuramoto, Kento Tazawa, Takashi Okiji
This study examined the effects and mechanisms of strontium ranelate (SrRn)-a drug used to treat osteoporosis-on the proliferation and differentiation/mineralization of cloned dental pulp-like cells (mouse dental papillae cells; MDPs). It also determined whether topical application of SrRn to exposed dental pulp tissue promotes the formation of mineralized tissue in vivo. The MDPs were cultured with or without SrRn, and cell proliferation, odonto-/osteoblastic gene expression, mineralized nodule formation, and Akt phosphorylation were evaluated...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907810/asxl1-ezh2-mutations-promote-clonal-expansion-of-neoplastic-hsc-and-impair-erythropoiesis-in-pmf
#8
Ioanna Triviai, Silke Zeschke, Jan Rentel, Marios Spanakis, Theo Scherer, Razif Gabdoulline, Victoria Panagiota, Felicitas Thol, Michael Heuser, Carol Stocking, Nicolaus Kröger
Primary myelofibrosis (PMF) is a hematopoietic stem cell (HSC) disease, characterized by aberrant differentiation of all myeloid lineages and profound disruption of the bone marrow niche. PMF samples carry several mutations, but their cell origin and hierarchy in regulating the different waves of clonal and aberrant myeloproliferation from the prime HSC compartment is poorly understood. Genotyping of >2000 colonies from CD133+HSC and progenitors from PMF patients confirmed the complex genetic heterogeneity within the neoplastic population...
June 15, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/29907695/cancer-cells-copy-migratory-behavior-and-exchange-signaling-networks-via-extracellular-vesicles
#9
Sander C Steenbeek, Thang V Pham, Joep de Ligt, Anoek Zomer, Jaco C Knol, Sander R Piersma, Tim Schelfhorst, Rick Huisjes, Raymond M Schiffelers, Edwin Cuppen, Connie R Jimenez, Jacco van Rheenen
Recent data showed that cancer cells from different tumor subtypes with distinct metastatic potential influence each other's metastatic behavior by exchanging biomolecules through extracellular vesicles (EVs). However, it is debated how small amounts of cargo can mediate this effect, especially in tumors where all cells are from one subtype, and only subtle molecular differences drive metastatic heterogeneity. To study this, we have characterized the content of EVs shed in vivo by two clones of melanoma (B16) tumors with distinct metastatic potential...
June 14, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29907599/aggressive-b-cell-lymphomas-in-patients-with-myelofibrosis-receiving-jak1-2-inhibitor-therapy
#10
Edit Porpaczy, Sabrina Tripolt, Andrea Hoelbl-Kovacic, Bettina Gisslinger, Zsuzsanna Bago-Horvath, Emilio Casanova-Hevia, Emmanuelle Clappier, Thomas Decker, Sabine Fajmann, Daniela A Fux, Georg Greiner, Sinan Gueltekin, Gerwin Heller, Harald Herkner, Gregor Hoermann, Jean-Jacques Kiladjian, Thomas Kolbe, Christoph Kornauth, Maria-Theresa Krauth, Robert Kralovics, Leonhard Muellauer, Mathias Mueller, Michaela Prchal-Murphy, Eva Maria Putz, Emmanuel Raffoux, Ana-Iris Schiefer, Klaus Schmetterer, Christine Schneckenleithner, Ingrid Simonitsch-Klupp, Cathrin Skrabs, Wolfgang R Sperr, Philipp Bernhard Staber, Birgit Strobl, Peter Valent, Ulrich Jaeger, Heinz Gisslinger, Veronika Sexl
Inhibition of Janus-kinase 1/2 (JAK1/2) is a mainstay to treat myeloproliferative neoplasms (MPN). Sporadic observations reported the co-incidence of B-cell non-Hodgkin lymphomas during treatment of MPN with JAK1/2 inhibitors. We assessed 626 MPN patients including 69 with myelofibrosis receiving JAK1/2 inhibitors for lymphoma development. B-cell lymphomas evolved in 4/69 patients (5.8%) upon JAK1/2 inhibition compared to 2/557 (0.36%) with conventional treatment (16-fold increased risk). A similar 15-fold increase was observed in an independent cohort of 929 MPN patients...
June 14, 2018: Blood
https://www.readbyqxmd.com/read/29907536/methicillin-resistant-staphylococcus-aureus-nasal-carriage-and-infection-among-patients-with-diabetic-foot-ulcer
#11
Shin-Yi Lin, Nai-Yu Lin, Yu-Yao Huang, Chi-Chun Hsieh, Yhu-Chering Huang
PURPOSE: To evaluate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage in patients with diabetic foot ulcer (DFU) in Taiwan, and to assess the concordance between colonizing and clinical MRSA isolates from the patients. METHOD: A total of 354 nasal specimens were collected from 112 to 242 diabetic patients with and without foot ulcer, respectively. MRSA clinical isolates from DFU wound cultures were collected for comparison. RESULTS: Nasal carriage rate of S...
June 4, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29907191/molecular-characterization-and-allergenicity-potential-of-triosephosphate-isomerase-from-sarcoptes-scabiei
#12
Ran He, Haojie Zhang, Nengxing Shen, Cheng Guo, Yongjun Ren, Yue Xie, Xiaobin Gu, Weimin Lai, Xuerong Peng, Guangyou Yang
Scabies is an allergic skin disease that affects millions of mammals worldwide, including humans. It is a neglected tropical disease that represents a significant public health threat, particularly in economically disadvantaged populations. An effective vaccine is not currently available, and the exact mode of pathogenesis remains unclear. Herein, we identified, cloned and recombinantly expressed triosephosphate isomerase from Sarcoptes scabiei (S. scabiei). Immunohistochemical analyses showed that S. scabiei triosephosphate isomerase (Ss-TIM) is localized in the legs and chewing mouthparts of mites, and in infected rabbit skin (keratinized skin and embedded mites)...
June 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29907095/a-case-of-immunotactoid-glomerulopathy-with-false-negative-igg-staining
#13
Shuma Hirashio, Takahiro Arima, Ayaka Satoh, Kouichi Mandai, Shigeo Hara, Takao Masaki
BACKGROUND: Immunotactoid glomerulopathy (ITG) is a rare glomerulonephritis characterized by microtubular deposits. Immunofluorescence findings are necessary to differentiate ITG from other proliferative glomerular diseases. The characteristic tubular structure on electron microscopy is essential for a definitive diagnosis, and the diameter of the structure has been traditionally used for differentiating between ITG and other types of glomerulonephritis with organized deposits. In recent years, the disease concept of monoclonal gammopathy of renal significance, which is associated with M protein produced by plasma cell tumors, has been proposed...
June 15, 2018: BMC Nephrology
https://www.readbyqxmd.com/read/29906624/features-of-chicken-egg-yolk-immunoglobulin-igy-against-the-infection-of-red-spotted-grouper-nervous-necrosis-virus
#14
Lizhu Yi, Zhendong Qin, Hanzuo Lin, Yang Zhou, Jiabo Li, Zhen Xu, Sarath Babu V, Li Lin
Red-spotted grouper nervous necrosis virus (RGNNV) is one of the most important viruses which mainly infects the larva of marine and freshwater fish with high mortality and affects the fishery industry worldwide. Currently, there are no effective vaccines available for the fish larva infected with NNV. Immunoglobulin yolk (IgY) origin of oviparous animals is passed from the blood serum and concentrated in the egg yolk. With the advantages of high yield, cost-effectiveness, and high stability, IgY can be widely used in passive immunization, especially in young animals in which adaptive immunity is not fully developed...
June 12, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29906414/guide-rna-selection-for-crispr-cas9-transfections-in-plasmodium-falciparum
#15
Jose M Ribeiro, Meera Garriga, Nicole Potchen, Anna K Crater, Ankit Gupta, Daisuke Ito, Sanjay A Desai
CRISPR-Cas9 mediated genome editing is addressing key limitations in the transfection of malaria parasites. While this method has already simplified the needed molecular cloning and reduced the time required to generate mutants in the human pathogen Plasmodium falciparum, optimal selection of required guide RNAs and guidelines for successful transfections have not been well characterized, leading workers to use time-consuming trial and error approaches. We used a genome-wide computational approach to create a comprehensive and publicly accessible database of possible guide RNA sequences in the P...
June 12, 2018: International Journal for Parasitology
https://www.readbyqxmd.com/read/29905780/isolation-and-functional-characterization-of-cslsi2-a-cucumber-silicon-efflux-transporter-gene
#16
Hao Sun, Yaoke Duan, Xiaocui Qi, Liyang Zhang, Heqiang Huo, Haijun Gong
Background and Aims: Silicon has been proven to exert beneficial effects on plant growth and stress tolerance, and silicon accumulation varies among different plant species. Cucumber (Cucumis sativus) is a widely used dicot model for silicon accumulation, but little is known about the molecular mechanism of its silicon uptake. Previously, we isolated and characterized CsLsi1, a silicon influx transporter gene from cucumber. In this study, we cloned a putative silicon efflux transporter gene, CsLsi2, from cucumber and investigated its role in silicon uptake...
June 14, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29905574/mechanisms-leading-to-t-cell-activation-in-drug-hypersensitivity
#17
Xiaoli Meng, Daniel Yerly, Dean J Naisbitt
PURPOSE OF REVIEW: Delayed-type or nonimmediate drug hypersensitivity reactions often involve the activation of drug-specific T cells. As such, the molecular initiating event is an interaction between HLA proteins, HLA-binding peptides and the drug. For many years, the formation of covalently modified drug protein adducts was assumed to be a prerequisite for T-cell activation. The purpose of this article is to review recent studies using human PBMC, T-cell lines and clones, which show that drugs are in fact loaded onto HLA molecules in different forms to activate T cells...
June 13, 2018: Current Opinion in Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29905572/cloning-expression-and-characterisation-of-a-cysteine-protease-from-trichinella-spiralis
#18
Yan Yan Song, Li Ang Wang, Hua Na Ren, Xin Qi, Ge Ge Sun, Ruo Dan Liu, Peng Jiang, Xi Zhang, Jing Cui, Zhong Quan Wang
Cysteine protease is a superfamily of widespread proteolytic enzymes and plays a major role in larval invasion, migration, exsheathing, survival and immune evasion in parasites. In the present study, the gene coding cysteine proteinase of the nematode Trichinella spiralis (Owen, 1835) was cloned into pQE-80L and subsequently expressed in E. coli JM109. The rTsCP was purified and its antigenicity was identified by Western blot and ELISA. Using anti-rTsCP serum the native TsCP was identified in muscle larval crude proteins...
May 29, 2018: Folia Parasitologica
https://www.readbyqxmd.com/read/29905492/lrhmgb1-shares-structural-similarities-with-human-hmgb1-and-its-expression-is-induced-in-bacterial-infection-antiviral-vaccination-and-pathogen-associated-molecular-patterns-stimulation
#19
Saswati S Lenka, Mahismita Paichha, Madhubanti Basu, Mrinal Samanta
The high-mobility group box 1 (HMGB1) protein is a highly conserved nonhistone chromosomal protein ubiquitously present in almost all cell types. In the nucleus, it facilitates DNA repair and replication, V(D)J recombination, stabilization of nucleosome, and in the cytoplasm, it regulates autophagy and apoptosis. In addition to these intracellular functions, HMGB1 also facilitates activation of innate immune responses and plays key roles in host defense. To investigate its role in fish, we cloned and characterized HMGB1 in Labeo rohita (LrHMGB1), the most important freshwater fish species in the Indian subcontinent...
June 15, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/29905035/a-cstu-ts1-regulatory-module-promotes-fruit-tubercule-formation-in-cucumber
#20
Sen Yang, Changlong Wen, Bin Liu, Yanling Cai, Shudan Xue, Ezra S Bartholomew, Mingming Dong, Chen Jian, Shuo Xu, Ting Wang, Wenzhu Qi, Jinan Pang, Dehua Ma, Xingwang Liu, Huazhong Ren
The fruit epidermal features such as the size of tubercules are important fruit quality traits for cucumber production. But the mechanisms underlying tubercule formation remain elusive. Here, tubercule size locus CsTS1 was identified by map-based cloning, and was found to encode an oleosin protein. Allelic variation was identified in the promoter region of CsTS1, resulting in low expression of CsTS1 in all 22 different small-warty or non-warty cucumber lines. High CsTS1 expression levels were closely correlated with increased fruit tubercule size among 44 different cucumber lines...
June 14, 2018: Plant Biotechnology Journal
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