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https://www.readbyqxmd.com/read/28107780/myb12-and-myb22-play-essential-roles-in-proanthocyanidin-and-flavonol-synthesis-in-red-fleshed-apple-malus-sieversii-f-niedzwetzkyana
#1
Nan Wang, Haifeng Xu, Shenghui Jiang, Zongying Zhang, Ninglin Lu, Huarong Qiu, Changzhi Qu, Yicheng Wang, Shujing Wu, Xuesen Chen
Flavonoids are major polyphenol compounds in plant secondary metabolism. Compared with cultivated apples, wild red-fleshed apples (Malus sieversii f. niedzwetzkyana) are excellent resources because of their high flavonoid content. In this work, R6R6, R6R1 and R1R1 genotypes were identified in an F1 segregating population of M. sieversii f. niedzwetzkyana. Significant differences in flavonoid compositions and contents were detected among the three genotypes by UPLC-ESI-TOF/MS analysis. Furthermore, two putative flavonoid-related genes encoding R2R3-MYB transcription factors, designated MYB12 and MYB22, were cloned and characterized...
January 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28107425/using-an-inducible-promoter-of-a-gene-encoding-penicillium-verruculosum-glucoamylase-for-production-of-enzyme-preparations-with-enhanced-cellulase-performance
#2
Alexander G Bulakhov, Pavel V Volkov, Aleksandra M Rozhkova, Alexander V Gusakov, Vitaly A Nemashkalov, Aidar D Satrutdinov, Arkady P Sinitsyn
BACKGROUND: Penicillium verruculosum is an efficient producer of highly active cellulase multienzyme system. One of the approaches for enhancing cellulase performance in hydrolysis of cellulosic substrates is to enrich the reaction system with β -glucosidase and/or accessory enzymes, such as lytic polysaccharide monooxygenases (LPMO) displaying a synergism with cellulases. RESULTS: Genes bglI, encoding β-glucosidase from Aspergillus niger (AnBGL), and eglIV, encoding LPMO (formerly endoglucanase IV) from Trichoderma reesei (TrLPMO), were cloned and expressed by P...
2017: PloS One
https://www.readbyqxmd.com/read/28107412/nasal-carriage-of-staphylococcus-aureus-among-children-in-the-ashanti-region-of-ghana
#3
Daniel Eibach, Michael Nagel, Benedikt Hogan, Clinton Azuure, Ralf Krumkamp, Denise Dekker, Mike Gajdiss, Melanie Brunke, Nimako Sarpong, Ellis Owusu-Dabo, Jürgen May
BACKGROUND: Nasal carriage with Staphylococcus aureus is a common risk factor for invasive infections, indicating the necessity to monitor prevalent strains, particularly in the vulnerable paediatric population. This surveillance study aims to identify carriage rates, subtypes, antimicrobial susceptibilities and virulence markers of nasal S. aureus isolates collected from children living in the Ashanti region of Ghana. METHODS: Nasal swabs were obtained from children < 15 years of age on admission to the Agogo Presbyterian Hospital between April 2014 and January 2015...
2017: PloS One
https://www.readbyqxmd.com/read/28107386/regulation-of-pi-2b-pilus-expression-in-hypervirulent-streptococcus-agalactiae-st-17-bm110
#4
Bruno Périchon, Noémi Szili, Laurence du Merle, Isabelle Rosinski-Chupin, Myriam Gominet, Samuel Bellais, Claire Poyart, Patrick Trieu-Cuot, Shaynoor Dramsi
The widely spread Streptococcus agalactiae (also known as Group B Streptococcus, GBS) "hypervirulent" ST17 clone is strongly associated with neonatal meningitis. The PI-2b locus is mainly found in ST17 strains but is also present in a few non ST17 human isolates such as the ST-7 prototype strain A909. Here, we analysed the expression of the PI-2b pilus in the ST17 strain BM110 as compared to the non ST17 A909. Comparative genome analyses revealed the presence of a 43-base pair (bp) hairpin-like structure in the upstream region of PI-2b operon in all 26 ST17 genomes, which was absent in the 8 non-ST17 strains carrying the PI-2b locus...
2017: PloS One
https://www.readbyqxmd.com/read/28106726/discrepancy-between-hepatitis-c-virus-genotypes-and-ns4-based-serotypes-association-with-their-subgenomic-sequences
#5
Nan Nwe Win, Shingo Nakamoto, Tatsuo Kanda, Hiroki Takahashi, Azusa Takahashi-Nakaguchi, Shin Yasui, Masato Nakamura, Shuang Wu, Fumio Imazeki, Shigeru Mikami, Osamu Yokosuka, Tohru Gonoi, Hiroshi Shirasawa
Determination of hepatitis C virus (HCV) genotypes plays an important role in the direct-acting agent era. Discrepancies between HCV genotyping and serotyping assays are occasionally observed. Eighteen samples with discrepant results between genotyping and serotyping methods were analyzed. HCV serotyping and genotyping were based on the HCV nonstructural 4 (NS4) region and 5'-untranslated region (5'-UTR), respectively. HCV core and NS4 regions were chosen to be sequenced and were compared with the genotyping and serotyping results...
January 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106276/crispr-cas9-mediated-deletion-of-c1eis-inhibits-chicken-embryonic-stem-cell-differentiation-into-male-germ-cells-gallus-gallus
#6
Qisheng Zuo, Kai Jin, Yingjie Wang, Jiuzhou Song, Yani Zhang, Bichun Li
We previously found that C1EIS is preferentially expressed in Chicken spermatogonial stem cells (SSCs) by RNA sequencing (RNA-seq), so our current study focused on C1EIS's role in Chicken embryonic stem cells (ESCs) differentiation into male germ cells. We constructed a CRISPR/Cas9 vector targeting C1EIS. T7 endonuclease I (T7EI) digestion method and sequencing of TA cloning were used to detect the knock-out efficiency of the Single guide RNA (sgRNA) after the cas9/gRNA vector transfected into D fibroblasts 1(DF-1), ESCs and Chicken embryos...
January 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28106258/t-cell-clones-in-human-trichinellosis-evidence-for-a-mixed-th1-th2-response
#7
C Della Bella, M Benagiano, M De Gennaro, M A Gomez-Morales, A Ludovisi, S D'Elios, S Luchi, E Pozio, M M D'Elios, F Bruschi
: In humans, studies on the cellular immune response against Trichinella are scarce. Aim of this study was to characterize the cytokine profile of T cells specific for Trichinella britovi in trichinellosis patients. PATIENTS AND METHODS: Peripheral blood mononuclear cells (PBMC) were obtained from five patients involved in a trichinellosis outbreak caused by Trichinella britovi, which occurred in 2013 in Tuscany (Italy). All the patients resulted positive for Trichinella-specific IgG, IgE and presented eosinophilia...
January 20, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28106148/complete-genome-sequencing-elucidates-outbreak-dynamics-of-ca-mrsa-usa300-st8-spa-t008-in-an-academic-hospital-of-paramaribo-republic-of-suriname
#8
Artur J Sabat, Sandra M Hermelijn, Viktoria Akkerboom, Amadu Juliana, John E Degener, Hajo Grundmann, Alexander W Friedrich
We report the investigation of an outbreak situation of methicillin-resistant Staphylococcus aureus (MRSA) that occurred at the Academic Hospital Paramaribo (AZP) in the Republic of Suriname from April to May 2013. We performed whole genome sequencing with complete gap closure for chromosomes and plasmids on all isolates. The outbreak involved 12 patients and 1 healthcare worker/nurse at the AZP. In total 24 isolates were investigated. spa typing, genome-wide single nucleotide polymorphism (SNP) analysis, ad hoc whole genome multilocus sequence typing (wgMLST), stable core genome MLST (cgMLST) and in silico PFGE were used to determine phylogenetic relatedness and to identify transmission...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28106071/development-of-an-efficient-screening-system-to-identify-novel-bone-metabolism-related-genes-using-the-exchangeable-gene-trap-mutagenesis-mouse-models
#9
Syuji Kurogi, Tomohisa Sekimoto, Taro Funamoto, Tomomi Ota, Shihoko Nakamura, Takuya Nagai, Mai Nakahara, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Etsuo Chosa
Despite numerous genetic studies on bone metabolism, understanding of the specific mechanisms is lacking. We developed an efficient screening system to identify novel genes involved in bone metabolism using mutant mouse strains registered with the Exchangeable Gene Trap Clones (EGTC) database. From 1278 trap clones in the EGTC database, 52 candidate lines were selected in the first screening, determined based on "EST profile", "X-gal", "Related article", and "Novel gene". For the second screening, bone morphometric analysis, biomechanical strength analysis, bone X-gal staining, etc...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28105864/expression-and-purification-of-l-asparaginase-from-escherichia-coli-and-the-inhibitory-effects-of-cyclic-dipeptides
#10
Yanan Zhang, Dan Li, Yan Li
L-asparaginase, a key enzyme involved in nitrogen metabolism, is an effective anti-tumour agent. Cyclic dipeptides, a group of compounds, contain several important biological functions. In this paper, we proposed a novel method for L-asparaginase expression and purification from Echerichia coli and determined the effect of cyclic dipeptides on the enzymatic activity of recombinant L-asparaginase. The gene ansB encoding L-asparaginase was amplified from the genome of E. coli BL21 (DE3) by polymerase chain reaction and sub-cloned into pET-15b vector to construct expressing plasmid pET-15b-ansB...
January 20, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28105799/molecular-characterization-and-functional-analysis-of-a-piscidin-gene-in-large-yellow-croaker-larimichthys-crocea
#11
Jing Yang, Xin-Jiang Lu, Fang-Chao Chai, Jiong Chen
The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-like type 3 (Lcpis5lt3) in large yellow croaker (Larimichthys crocea). Multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). The phylogenetic tree confirmed that Lcpis5lt3 and large yellow croaker piscidin-5-like proteins were grouped together to form a branch...
November 18, 2016: Zool Res
https://www.readbyqxmd.com/read/28105534/construction-of-a-novel-lipolytic-fusion-biocatalyst-gdest-lip-for-industrial-application
#12
Renata Gudiukaite, Mikas Sadauskas, Audrius Gegeckas, Vilius Malunavicius, Donaldas Citavicius
The gene encoding esterase (GDEst-95) from Geobacillus sp. 95 was cloned and sequenced. The resulting open reading frame of 1497 nucleotides encoded a protein with calculated molecular weight of 54.7 kDa, which was classified as a carboxylesterase with an identity of 93-97% to carboxylesterases from Geobacillus bacteria. This esterase can be grouped into family VII of bacterial lipolytic enzymes, was active at broad pH (7-12) and temperature (5-85 °C) range and displayed maximum activity toward short acyl chain p-nitrophenyl (p-NP) esters...
January 19, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28105183/a-novel-anti-adhesion-peptide-%C3%AE-3-inhibits-hepatocellular-carcinoma-activity-in-vitro-and-in-vivo
#13
Songmei Wang, Jun Zhu, Yinkun Liu
The present study aimed to investigate the blocking of tumor cell adhesion to the extracellular matrix, the prevention of tumor metastasis by the β peptide trimer β3, as well as the influence of β3 on the recurrence and survival time of hepatocellular carcinoma (HCC) nude mice model LCI-D20 after early resection. To this end, the DNA fragment of the β3 peptide (DLYYLMDLSYSMKGGDLYYLMDLSYSMKGGDLYYLMDLSYSMK) was cloned into the expression vector pET-His and the fusion protein His-β3 was expressed in E. coli BL21 (DE3) plysS...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105180/screening-specific-polypeptides-of-breast-cancer-stem-cells-from-a-phage-display-random-peptide-library
#14
Fei Liu, Chun-Ling Qi, Mian Kong, Ting-Ting Liu, Lei Li, Bao-Jiang Li
The present study aimed to identify polypeptides that specifically bond to breast cancer stem cells from a phage display random 12 peptide library, in addition to the affinity and specificity of polypeptides. A phage display random 12 peptide library was screened using breast cancer stem cells as targets isolated from the MDA-MB-231 cell line using the serum-free culture technique with hs578bst and MDA-MB-231 cells as subtract-screening cells. Positive and specific binding clones were amplified and sent for sequencing...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105164/mir-219-5p-inhibits-proliferation-and-clonogenicity-in-chordoma-cells-and-is-associated-with-tumor-recurrence
#15
Wei Wei, Qiuhang Zhang, Zhenlin Wang, Bo Yan, Yanjun Feng, Pu Li
Chordoma is a rare malignant bone tumor that is usually localized to the skull base, vertebral column and sacrum. The transcription factor brachyury, which is encoded by the T gene, has a critical role in the development and progression of chordoma, although the mechanisms underlying brachyury regulation remain unclear. The aim of the current study was to identify and characterize microRNAs (miRs) that regulate brachyury expression in chordoma. MicroRNAs that target brachyury were predicted using miRanda and TargetScan...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105149/t-14-18-q32-q21-in-chronic-lymphocytic-leukemia-patients-report-of-two-cases-and-a-literature-review
#16
Weifeng Chen, Yi Miao, Rong Wang, Yujie Wu, Hairong Qiu, Wei Xu, Jianyong Li, Lei Fan, Xin Xu
The chromosomal abnormality t(14;18)(q32;q21) is most commonly associated with germinal center-derived B-cell lymphomas, particularly follicular lymphoma (FL). Generally, it is considered a hallmark of FL. The t(14;18)(q32;q21) translocation is rare in chronic lymphocytic leukemia (CLL) and its prognostic significance remains unclear. In the present study, two cases of CLL with t(14;18)(q32;q21) were diagnosed using conventional cytogenetic analysis and fluorescence in situ hybridization. Both patients presented with leukemia and the morphological features and immunophenotypes were typical of CLL...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28104812/antibody-secreting-plasma-cells-persist-for-decades-in-human-intestine
#17
Ole J B Landsverk, Omri Snir, Raquel Bartolomé Casado, Lisa Richter, Jeff E Mold, Pedro Réu, Rune Horneland, Vemund Paulsen, Sheraz Yaqub, Einar Martin Aandahl, Ole M Øyen, Hildur Sif Thorarensen, Mehran Salehpour, Göran Possnert, Jonas Frisén, Ludvig M Sollid, Espen S Baekkevold, Frode L Jahnsen
Plasma cells (PCs) produce antibodies that mediate immunity after infection or vaccination. In contrast to PCs in the bone marrow, PCs in the gut have been considered short lived. In this study, we studied PC dynamics in the human small intestine by cell-turnover analysis in organ transplants and by retrospective cell birth dating measuring carbon-14 in genomic DNA. We identified three distinct PC subsets: a CD19(+) PC subset was dynamically exchanged, whereas of two CD19(-) PC subsets, CD45(+) PCs exhibited little and CD45(-) PCs no replacement and had a median age of 11 and 22 yr, respectively...
January 19, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28104777/naturally-occurring-inlb-variants-that-support-intragastric-listeria-monocytogenes-infection-in-mice
#18
Konstantin Sobyanin, Elena Sysolyatina, Mikhail Krivozubov, Yaroslava Chalenko, Anna Karyagina, Svetlana Ermolaeva
Listeria monocytogenes is a causative agent of foodborne infection in humans and animals. The virulence factor InlB interacts with mammalian receptor c-Met via its internalin domain to provide L. monocytogenes invasion in non-professional phagocytes. Naturally occurring InlB internalin domain variants form 4 subclusters on the maximal likelihood tree. Four variants belonging to distinct subclusters were cloned into the vector carrying 3' and 5'-flanking sequences to restore full length inlB and expressed in the L...
January 18, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28104537/correlation-between-classic-driver-oncogene-mutations-in-egfr-alk-or-ros1-and-22c3-pd-l1-%C3%A2-50-expression-in-lung-adenocarcinoma
#19
Deepa Rangachari, Paul A VanderLaan, Meghan Shea, Xiuning Le, Mark S Huberman, Susumu S Kobayashi, Daniel B Costa
INTRODUCTION: Targeted somatic genomic analysis (EGFR, ALK and ROS1) and PD-L1 tumor proportion score (TPS) by immunohistochemistry (IHC) are used for selection of 1(st)-line therapies in advanced lung cancer; however, the frequency of overlap of these biomarkers in routine clinical practice is poorly reported. METHODS: We retrospectively probed the first 71 lung adenocarcinoma-patient pairs from our institution analyzed for PD-L1 IHC using the clone 22C3 pharmDx kit and evaluated co-occurrence of genomic aberrations along with clinical-pathologic characteristics...
January 16, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/28103877/rhodococcus-erythropolis-as-a-host-for-expression-secretion-and-glycosylation-of-mycobacterium-tuberculosis-proteins
#20
Antonio J Vallecillo, Cristina Parada, Pedro Morales, Clara Espitia
BACKGROUND: Glycosylation is one of the most abundant posttranslational polypeptide chain modification in nature. Although carbohydrate modification of protein antigens from many microbial pathogens constitutes important components of B cell epitopes, the role in T cell immunity is not completely understood. There is growing evidence about the importance of these modifications in host bacteria interactions in tuberculosis. It is known, that the sugars present in some Mycobacterium tuberculosis glycoproteins play an important role in both humoral and cellular immune response against the pathogen...
January 19, 2017: Microbial Cell Factories
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