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https://www.readbyqxmd.com/read/29040919/functional-analyses-of-the-interaction-of-chicken-interleukin-23-subunit-p19-with-il-12-subunit-p40-to-form-the-il-23-complex
#1
Anh Duc Truong, Cong Thanh Hoang, Yeojin Hong, Janggeun Lee, Kyungbaek Lee, Hyun S Lillehoj, Yeong Ho Hong
This study represents the first description of the cloning of chicken IL-23p19 (ChIL-23α) and the function of the IL-23 complex in birds. Multiple alignment of ChIL-23α with other known IL-23α amino acid sequences revealed regions of amino acid conservation. The homologies of ChIL-23α, IL-12p35, and similar mammalian subunits ranged between 26% and 42%. ChIL-23α consisted of four exons and three introns; similar to those in humans and mice, and limited conservation of synteny between the human and chicken genomes was observed...
October 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29040878/c-phycocyanin-supplementation-during-in%C3%A2-vitro-maturation-enhances-pre-implantation-developmental-competence-of-parthenogenetic-and-cloned-embryos-in-pigs
#2
Shuang Liang, Jing Guo, Yong Xun Jin, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim
C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL)...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29040781/further-insights-into-the-components-of-resistance-to-ophiostoma-novo-ulmi-in-ulmus-minor-hydraulic-conductance-stomatal-sensitivity-and-bark-dehydration
#3
Pilar Pita, Jesús Rodríguez-Calcerrada, David Medel, Luis Gil
Dutch elm disease (DED) is a vascular disease that has killed over 1 billion elm trees. The pathogen spreads throughout the xylem network triggering vessel blockage, which results in water stress, tissue dehydration and extensive leaf wilting in susceptible genotypes. We investigated the differences between four Ulmus minor Mill. clones of contrasting susceptibility to Ophiostoma novo-ulmi Brasier regarding morphological, anatomical and physiological traits affecting water transport, in order to gain a better understanding of the mechanisms underlying DED susceptibility...
October 13, 2017: Tree Physiology
https://www.readbyqxmd.com/read/29040754/development-of-unique-cytotoxic-chimeric-antigen-receptors-based-on-human-scfv-targeting-b7h6
#4
Casey K Hua, Albert T Gacerez, Charles L Sentman, Margaret E Ackerman
As a stress-inducible natural killer (NK) cell ligand, B7H6 plays a role in innate tumor immunosurveillance and is a fairly tumor selective marker expressed on a variety of solid and hematologic cancer cells. Here, we describe the isolation and characterization of a new family of single chain fragment variable (scFv) molecules targeting the human B7H6 ligand. Through directed evolution of a yeast surface displayed non-immune human-derived scFv library, eight candidates comprising a single family of clones differing by up to four amino acid mutations and exhibiting nM avidities for soluble B7H6-Ig were isolated...
October 11, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/29040741/identification-of-new-protein-protein-and-protein-dna-interactions-linked-with-wood-formation-in-populus-trichocarpa
#5
H Earl Petzold, Stephen B Rigoulot, Chengsong Zhao, Bidisha Chanda, Xiaoyan Sheng, Mingzhe Zhao, Xiaoyan Jia, Allan W Dickerman, Eric P Beers, Amy M Brunner
Cellular processes, such as signal transduction and cell wall deposition, are organized by macromolecule interactions. Experimentally determined protein-protein interactions (PPIs) and protein-DNA interactions (PDIs) relevant to woody plant development are sparse. To begin to develop a Populus trichocarpa Torr. & A. Gray wood interactome, we applied the yeast-two-hybrid (Y2H) assay in different ways to enable the discovery of novel PPIs and connected networks. We first cloned open reading frames (ORFs) for 361 genes markedly upregulated in secondary xylem compared with secondary phloem and performed a binary Y2H screen with these proteins...
October 10, 2017: Tree Physiology
https://www.readbyqxmd.com/read/29040564/n-cadherin-identifies-human-endometrial-epithelial-progenitor-cells-by-in-vitro-stem-cell-assays
#6
Hong P T Nguyen, L Xiao, James A Deane, Ker-Sin Tan, Fiona L Cousins, Hirotaka Masuda, Carl N Sprung, Anna Rosamilia, Caroline E Gargett
STUDY QUESTION: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays? SUMMARY ANSWER: N-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structures in vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium...
September 27, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040422/pharmacogenetics-a-general-review-on-progress-to-date
#7
Ann K Daly
Background: Pharmacogenetics is not a new subject area but its relevance to drug prescribing has become clearer in recent years due to developments in gene cloning and DNA genotyping and sequencing. Sources of data: There is a very extensive published literature concerned with a variety of different genes and drugs. Areas of agreement: There is general agreement that pharmacogenetic testing is essential for the safe use of drugs such as the thiopurines and abacavir...
October 11, 2017: British Medical Bulletin
https://www.readbyqxmd.com/read/29040308/thermostable-proteins-bioprocesses-the-activity-of-restriction-endonuclease-methyltransferase-from-thermus-thermophilus-rm-tthhb27i-cloned-in-escherichia-coli-is-critically-affected-by-the-codon-composition-of-the-synthetic-gene
#8
Daria Krefft, Aliaksei Papkov, Agnieszka Zylicz-Stachula, Piotr M Skowron
Obtaining thermostable enzymes (thermozymes) is an important aspect of biotechnology. As thermophiles have adapted their genomes to high temperatures, their cloned genes' expression in mesophiles is problematic. This is mainly due to their high GC content, which leads to the formation of unfavorable secondary mRNA structures and codon usage in Escherichia coli (E. coli). RM.TthHB27I is a member of a family of bifunctional thermozymes, containing a restriction endonuclease (REase) and a methyltransferase (MTase) in a single polypeptide...
2017: PloS One
https://www.readbyqxmd.com/read/29036991/-molecular-epidemiology-of-carbapenem-resistant-klebsiella-pneumoniae-in-a-hospital-in-beijing
#9
D K Chen, H J Zhou
Objective: To reveal the molecular epidemiological characteristics of Carbapenem-Resistant Klebsiella pneumoniae (CRKP) isolated from a three level teaching hospital in Beijing. Methods: Pulsed field gel electrophoresis (PFGE) was carried out to subtyping 375 CRKP isolated in that hospital between May 2010 and October 2015. Fifteen strains were chose based on the PFGE patterns to be analyzed by multi-locus sequence typing (MLST) and detection of carbapenem-resistance genes. One strain (A1502) was selected for whole genome sequencing and analyzing...
October 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29036807/obtaining-and-characterization-of-monoclonal-antibodies-against-recombinant-extracellular-domain-of-human-epidermal-growth-factor-receptor-2
#10
Sarina Nurgul, Abeldenov Sailau, Turgimbayeva Aigerim, Zhylkibayev Assylbek, Ramankulov Erlan, Khassenov Bekbolat, Eskendirova Saule
Human epidermal growth factor receptor 2 (HER2) is an important biomarker for detection and treatment of different types of cancers such as breast, ovarian, stomach cancer. In this study, we developed a monoclonal antibody against the extracellular domain (ECD) of HER2 biomarker of breast cancer. For this purpose, the ECD-HER2 gene was amplified and cloned into an expression vector. Gene was generated in Escherichia coli BL21 (DE3) strain for expression of recombinant protein. The expressed protein was separated by SDS-PAGE and detected by anti-his monoclonal antibody in immunoblotting...
September 8, 2017: Human Antibodies
https://www.readbyqxmd.com/read/29036679/human-recombinant-fab-fragment-from-combinatorial-libraries-of-a-b-cell-lymphoma-patient-recognizes-core-protein-of-chondroitin-sulfate-proteoglycan-4
#11
Yoko Egami, Yuta Narushima, Motohiro Ohshima, Akira Yoshida, Naruki Yoneta, Yasufumi Masaki, Kunihiko Itoh
CD antigens are well known as therapeutic targets of B-cell lymphoma. To isolate therapeutic antibodies that recognize novel targets other than CD antigens, we constructed a phage display combinatorial antibody Fab library from bone marrow lymphocytes of B-cell lymphoma patient. To eliminate antibodies reactive with known B-cell lymphoma antigen, non-hematopoietic and patient's sera reactive HeLaS3 cells was selected as a target of whole cell panning. Five rounds of panning against live HeLaS3 cells retrieved single Fab clone, termed AHSA (Antibody to HeLa Surface Antigen)...
September 28, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29036617/arrayed-mutant-haploid-embryonic-stem-cell-libraries-facilitate-phenotype-driven-genetic-screens
#12
Guang Liu, Xue Wang, Yufang Liu, Meili Zhang, Tao Cai, Zhirong Shen, Yuyan Jia, Yue Huang
Forward genetic screens using mammalian embryonic stem (ES) cells have identified genes required for numerous cellular processes. However, loss-of-function screens are more difficult to conduct in diploid cells because, in most cases, both alleles of a gene must be mutated to exhibit a phenotype. Recently, mammalian haploid ES cell lines were successfully established and applied to several recessive genetic screens. However, all these screens were performed in mixed pools of mutant cells and were mainly based on positive selection...
September 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036468/exponential-propagation-of-large-circular-dna-by-reconstitution-of-a-chromosome-replication-cycle
#13
Masayuki Su'etsugu, Hiraku Takada, Tsutomu Katayama, Hiroko Tsujimoto
Propagation of genetic information is a fundamental property of living organisms. Escherichia coli has a 4.6 Mb circular chromosome with a replication origin, oriC. While the oriC replication has been reconstituted in vitro more than 30 years ago, continuous repetition of the replication cycle has not yet been achieved. Here, we reconstituted the entire replication cycle with 14 purified enzymes (25 polypeptides) that catalyze initiation at oriC, bidirectional fork progression, Okazaki-fragment maturation and decatenation of the replicated circular products...
September 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036360/subcellular-localization-and-interactions-among-rubber-particle-proteins-from-hevea-brasiliensis
#14
Daniel Brown, Mistianne Feeney, Mathin Ahmadi, Chiara Lonoce, Roslinda Sajari, Alessandra Di Cola, Lorenzo Frigerio
Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER)...
September 25, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29036229/cloning-and-characterization-of-the-cerasus-humilis-sucrose-phosphate-synthase-gene-chsps1
#15
Juan Wang, Junjie Du, Xiaopeng Mu, Pengfei Wang
Sucrose is crucial to the growth and development of plants, and sucrose phosphate synthase (SPS) plays a key role in sucrose synthesis. To understand the genetic and molecular mechanisms of sucrose synthesis in Cerasus humilis, ChSPS1, a homologue of SPS, was cloned using RT-PCR. Sequence analysis showed that the open reading frame (ORF) sequence of ChSPS1 is 3174 bp in length, encoding a predicted protein of 1057 amino acids. The predicted protein showed a high degree of sequence identity with SPS homologues from other species...
2017: PloS One
https://www.readbyqxmd.com/read/29036211/orally-administered-taenia-solium-calreticulin-prevents-experimental-intestinal-inflammation-and-is-associated-with-a-type-2-immune-response
#16
Fela Mendlovic, Mayra Cruz-Rivera, Jose Alfredo Diaz-Gandarilla, Marco Antonio Flores-Torres, Guillermina Avila, Maria Perfiliev, Ana Maria Salazar, Lourdes Arriaga-Pizano, Patricia Ostrosky-Wegman, Ana Flisser
Intestinal helminth antigens are inducers of type 2 responses and can elicit regulatory immune responses, resulting in dampened inflammation. Several platyhelminth proteins with anti-inflammatory activity have been reported. We have identified, cloned and expressed the Taenia solium calreticulin (rTsCRT) and shown that it predominantly induces a type 2 response characterized by IgG1, IL-4 and IL-5 production in mice. Here, we report the rTsCRT anti-inflammatory activity in a well-known experimental colitis murine model...
2017: PloS One
https://www.readbyqxmd.com/read/29036193/gestational-age-related-patterns-of-amot-methylation-are-revealed-in-preterm-infant-endothelial-progenitors
#17
Giovanna Vinci, Christophe Buffat, Stéphanie Simoncini, Farid Boubred, Isabelle Ligi, Florent Dumont, Bernard Le Bonniec, Thierry Fournier, Daniel Vaiman, Françoise Dignat-George, Umberto Simeoni
OBJECTIVE: Preterm birth is associated with altered angiogenesis and with increased risk of cardiovascular dysfunction and hypertension at adulthood. We previously demonstrated that in preterm newborns circulating cord blood endothelial progenitor cells (ECFC), responsible for angio/vasculogenesis, are reduced in number and display altered angiogenic properties. Altered angiogenic function was associated with a decreased expression of pro-angiogenic genes, among which the AMOT gene which is a strong positive regulator of angiogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/29035848/animal-level-risk-factors-associated-with-babesia-and-theileria-infections-in-cattle-in-egypt
#18
Mohamed Abdo Rizk, Akram Salama, Shimaa Abd-El-Salam El-Sayed, Ahmed Elsify, Maged El-Ashkar, Hussam Ibrahim, Mohamed Youssef, Sabry El-Khodery
In present study, blood samples were collected randomly from 439 cows at three main regions of Egypt (northern, central and southern). Molecular diagnosis of Babesia and Theileria infections by PCR amplification of DNA (gene) fragments, then cloning and sequencing of the positive samples were conducted. A questionnaire was created to imply the assumed risk factors and logistic regression statistical analysis was carried out to appraise the potential factors on the animal level. The results revealed that 49 (11...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035787/identification-of-n-linked-glycosylation-sites-in-the-spike-protein-and-their-functional-impact-on-the-replication-and-infectivity-of-coronavirus-infectious-bronchitis-virus-in-cell-culture
#19
Jie Zheng, Yoshiyuki Yamada, To Sing Fung, Mei Huang, Raymond Chia, Ding Xiang Liu
Spike (S) glycoprotein on the viral envelope is the main determinant of infectivity. The S protein of coronavirus infectious bronchitis virus (IBV) contains 29 putative asparagine(N)-linked glycosylation sites. These post-translational modifications may assist in protein folding and play important roles in the functionality of S protein. In this study, we used bioinformatics tools to predict N-linked glycosylation sites and to analyze their distribution in IBV strains and variants. Among these sites, 8 sites were confirmed in the S protein extracted from partially purified virus particles by proteomics approaches...
October 13, 2017: Virology
https://www.readbyqxmd.com/read/29035625/chickens-with-humanized-immunoglobulin-genes-generate-antibodies-with-high-affinity-and-broad-epitope-coverage-to-conserved-targets
#20
Kathryn H Ching, Ellen J Collarini, Yasmina N Abdiche, Daniel Bedinger, Darlene Pedersen, Shelley Izquierdo, Rian Harriman, Lei Zhu, Robert J Etches, Marie-Cecile van de Lavoir, William D Harriman, Philip A Leighton
Transgenic animal platforms for the discovery of human monoclonal antibodies have been developed in mice, rats, rabbits and cows. The immune response to human proteins is limited in these animals by their tolerance to mammalian-conserved epitopes. To expand the range of epitopes that are accessible, we have chosen an animal host that is less phylogenetically related to humans. Specifically, we generated transgenic chickens expressing antibodies from immunoglobulin heavy and light chain loci containing human variable regions and chicken constant regions...
October 16, 2017: MAbs
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