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https://www.readbyqxmd.com/read/29679790/towards-quality-and-order-in-human-papillomavirus-research
#1
Laila Sara Arroyo Mühr, Carina Eklund, Joakim Dillner
The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, currently up to HPV225, belong to 5 different genera: alpha (65 types), beta (54 types), gamma (98 types), mu (3 types) and nu (1 type). Since 2014, 23 novel types have been established, 82.6% of which belong to the gamma genus...
April 18, 2018: Virology
https://www.readbyqxmd.com/read/29679757/molecular-characterization-of-cu-zn-sod-gene-in-asian-clam-corbicula-fluminea-and-mrna-expression-and-enzymatic-activity-modulation-induced-by-metals
#2
Xie Yanhai, Chen Hongbing, Zheng Shuangyan, Zhang Xiali, Mu Songniu
The copper/zinc superoxide dismutase (Cu/Zn SOD) could effectively eliminate reactive oxygen species (ROS). In this study, the cDNA sequence of Cu/Zn SOD from Corbicula fluminea (designated as CfCu/Zn SOD) was cloned by the rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of CfCu/Zn SOD was of 1288 bp, including a 465 bp ORF encoding a protein of 154 amino acids. Two SOD family signatures were identified in CfCu/Zn SOD amino acids sequence. Multiple sequence alignments indicated that CfCu/Zn SOD amino acid sequences exhibited high similarities to those of other species...
April 18, 2018: Gene
https://www.readbyqxmd.com/read/29679684/expression-profile-and-reproductive-regulation-of-apgwamide-in-pacific-abalone-haliotis-discus-hannai
#3
Kyeong Seop Kim, Tae Ha Kim, Mi Ae Kim, Jung Sick Lee, Young Chang Sohn
Neuropeptides in the central nervous system regulate reproductive activities in vertebrates. Ala-Pro-Gly-Trp-NH2 (APGWamide), a neuromediator expressed in the neural ganglia of mollusks, controls sexual maturation and reproduction. To clarify the role of APGWamide in sexual behavior regulation and gamete cell maturation in mollusks, we cloned the cDNA of APGWamide precursor (Hdh-APGWamide) and examined the spatiotemporal expression of the transcript in the Pacific abalone Haliotis discus hannai. The 222-amino acid sequence of the precursor deduced from the cDNA sequence showed typical features of gastropod APGWamide precursors...
April 18, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29679176/a-versatile-vector-toolkit-for-functional-analysis-of-rice-genes
#4
Feng He, Fan Zhang, Wenxian Sun, Yuese Ning, Guo-Liang Wang
BACKGROUND: Rice (Oryza sativa) is the main food for half of the world's population, and is considered the model for molecular biology studies of monocotyledon species. Although the rice genome was completely sequenced about 15 years ago, the function of most rice genes is still unknown. RESULTS: In this study, we developed a vector toolkit that contains 42 vectors for transient expression studies in rice protoplasts and stable expression analysis in transgenic rice...
April 20, 2018: Rice
https://www.readbyqxmd.com/read/29679043/identification-and-heterologous-expression-of-the-actinoallolide-biosynthetic-gene-cluster
#5
Yuki Inahashi, Taro Shiraishi, Akira Také, Atsuko Matsumoto, Yōko Takahashi, Satoshi Ōmura, Tomohisa Kuzuyama, Takuji Nakashima
Actinoallolides are anti-trypanosomal macrolides isolated from the secondary metabolites of two endophytic actinomycete strains, Actinoallomurus fulvus MK10-036 and K09-0307. A putative actinoallolide biosynthetic gene cluster was predicted from the genome sequence of the strain K09-0307. The gene cluster spans a contiguous 53 kb DNA region that comprises seven genes encoding three PKSs (aalA1, aalA2, and aalA3), cytochrome P450 (aalB), acyl-CoA dehydrogenase (aalC), crotonyl-CoA reductase (aalD), and TetR family regulator (aalR)...
April 20, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29678922/enzymatic-synthesis-of-6-sialyllactose-a-dominant-sialylated-human-milk-oligosaccharide-by-a-novel-exo-%C3%AE-sialidase-from-bacteroides-fragilis-nctc9343
#6
Longcheng Guo, Xiaodi Chen, Li Xu, Min Xiao, Lili Lu
Gut bacteria provide a rich source of glycosidases that can recognize and/or hydrolyze glycans for nutritions. Interestingly, some glycosidases are also found to catalyze transglycosylation reactions in vitro and thus can be used for oligosaccharide synthesis. In this work, six putative and one known exo -α-sialidase genes, including three from Bacteroides fragilis NCTC9343, three from Clostridium perfringens ATCC13124, and one known from Bifidobacterium bifidum JCM1254, were subjected to gene cloning and heterogeneous expression in Escherichia coli The recombinant enzymes were purified, characterized for substrate specificity, and screened for transglycosylation activity...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29678725/cloning-and-characterization-of-a-second-lamprey-pituitary-glycoprotein-hormone-thyrostimulin-gpa2-gpb5
#7
Krist N Hausken, Belen Tizo, Michal Shpilman, Shannon Barton, Wayne Decatur, David Plachetzki, Scott Kavanaugh, Sabah Ul-Hasan, Berta Levavi-Sivan, Stacia A Sower
A novel heterodimeric glycoprotein hormone (GpH) comprised of alpha (GpA2) and beta (GpB5) subunits was discovered in 2002 and called thyrostimulin for its ability to activate the TSH receptor in mammals, but its central function in vertebrates has not been firmly established. We report here the cloning and expression of lamprey (l)GpB5, and its ability to heterodimerize with lGpA2 to form a functional l-thyrostimulin. The full-length cDNA of lGpB5 encodes 174 amino acids with ten conserved cysteine residues and one glycosylation site that is conserved with other vertebrate GpB5 sequences...
April 17, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29678688/control-in-dormancy-or-eradication-of-cancer-stem-cells-mathematical-modeling-and-stability-issues
#8
Walid Djema, Catherine Bonnet, Frédéric Mazenc, Jean Clairambault, Emilia Fridman, Pierre Hirsch, François Delhommeau
OBJECTIVE: Modeling and analysis of cell population dynamics enhance our understanding of cancer. Here we introduce and explore a new model that may apply to many tissues. ANALYSES: An age-structured model describing coexistence between mutated and ordinary stem cells is developed and explored. The model is transformed into a nonlinear time-delay system governing the dynamics of healthy cells, coupled to a nonlinear differential-difference system describing dynamics of unhealthy cells...
April 17, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29678657/development-and-evaluation-of-an-optimal-human-single-chain-variable-fragment-derived-bcma-targeted-car-t-cell-vector
#9
Eric L Smith, Mette Staehr, Reed Masakayan, Ishan J Tatake, Terence J Purdon, Xiuyan Wang, Pei Wang, Hong Liu, Yiyang Xu, Sarah C Garrett-Thomson, Steven C Almo, Isabelle Riviere, Cheng Liu, Renier J Brentjens
B cell maturation antigen (BCMA) has recently been identified as an important multiple myeloma (MM)-specific target for chimeric antigen receptor (CAR) T cell therapy. In CAR T cell therapy targeting CD19 for lymphoma, host immune anti-murine CAR responses limited the efficacy of repeat dosing and possibly long-term persistence. This clinically relevant concern can be addressed by generating a CAR incorporating a human single-chain variable fragment (scFv). We screened a human B cell-derived scFv phage display library and identified a panel of BCMA-specific clones from which human CARs were engineered...
March 28, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29678299/molecular-epidemiology-of-klebsiella-pneumoniae-k1-and-k2-isolates-in-japan
#10
Sohei Harada, Yoshikazu Ishii, Tomoo Saga, Kotaro Aoki, Kazuhiro Tateda
Although severe infections caused by hypervirulent Klebsiella pneumoniae isolates, such as K1 isolates belonging to sequence type (ST) 23, have been a significant problem in Asian countries, epidemiology of these isolates in Japan remains unclear. We performed a nationwide molecular epidemiological study of K. pneumoniae K1 and K2 isolates in Japan. Of the 259K. pneumoniae isolates collected, 14 and 16 isolates were identified as capsular genotypes K1 and K2, respectively. All K1 isolates were ST23 or its closely related clones and showed high genetic similarity by pulsed-field gel electrophoresis (PFGE) and the DiversiLab system (DL)...
March 20, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29678226/multispectral-fluorescence-activated-cell-sorting-of-b-and-t-cell-subpopulations-from-equine-peripheral-blood
#11
Joy E Tomlinson, Bettina Wagner, M Julia B Felippe, Gerlinde R Van de Walle
Immune phenotyping of equine peripheral blood mononuclear cells (PBMC) is commonly described by single or double marker labeling, which limits complex phenotypic descriptions and subpopulation identification. Our objective was to develop a new multispectral flow cytometry protocol to identify and sort equine lymphocyte subpopulations using commercially available, pre-conjugated monoclonal antibodies to cell surface markers. Two clones of anti-equine CD8 (CVS8 and CVS21) were compared in combination with CD3...
May 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29677793/a-label-free-detection-of-ndei-endonuclease-activity-by-using-dna-templated-silver-nanoclusters
#12
Chunghyun Lee, Jongback Gang
Restriction endonucleases play an important role in genetic recombination, molecular cloning, clinical diagnosis, and pharmacological application in drug studies. In the present study, DNA-templated Ag+ nanoclusters (DNA-AgNCs) were used to detect NdeI endonuclease activity due to their facile synthesis, outstanding optical properties, and good biocompatibility. This study monitored fluorescence intensity of DNA-AgNCs to detect NdeI endonuclease activity and its inhibition in the presence of 5-fluorouracil under optimal conditions...
September 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29677589/generation-of-the-human-induced-pluripotent-stem-cell-hipsc-line-psmi002-a-from-a-patient-affected-by-the-jervell-and-lange-nielsen-syndrome-and-carrier-of-two-compound-heterozygous-mutations-on-the-kcnq1-gene
#13
Manuela Mura, Yee-Ki Lee, Monia Ginevrino, Rita Zappatore, Federica Pisano, Marina Boni, Federica Dagradi, Lia Crotti, Enza Maria Valente, Peter J Schwartz, Hung-Fat Tse, Massimiliano Gnecchi
We report the generation of human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a female patient carrier of the two compound heterozygous mutations c.568 C>T p.R190W (maternal allele), and c.1781 G>A p.R594Q (paternal allele) on the KCNQ1 gene, causing Jervell and Lange-Nielsen Syndrome (JLNS). To obtain hiPSCs, we used the classical approach of the four retroviruses each encoding for a reprogramming factor OCT4, SOX2, KLF4, cMYC. The obtained hiPSC clones display pluripotent stem cell characteristics, and differentiate into spontaneously beating cardiomyocytes (hiPSC-CMs)...
April 7, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29677577/dna-typing-of-birch-development-of-a-forensic-str-system-for-betula-pendula-and-betula-pubescens
#14
Monique Wesselink, Aleksandar Dragutinović, Jeroen W Noordhoek, Leonie Bergwerff, Irene Kuiper
Although botanical trace evidence is often encountered in case investigations, the utilization of such traces in forensic investigations is still limited. Development of a forensic STR system for the two species of Betula (birch) indigenous to and abundant in North West Europe is a step in enhancing the applicability of traces from these species. We describe six microsatellite markers developed for birch species in detail, including repeat structure, and we propose a nomenclature for the encountered alleles...
April 7, 2018: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29677349/repeat-associated-non-atg-ran-translation-in-fuchs-endothelial-corneal-dystrophy
#15
Elisabetta Soragni, Lina Petrosyan, Tommy A Rinkoski, Eric D Wieben, Keith H Baratz, Michael P Fautsch, Joel M Gottesfeld
Purpose: The strongest genetic association with Fuchs' endothelial corneal dystrophy (FECD) is the presence of an intronic (CTG·CAG)n trinucleotide repeat (TNR) expansion in the transcription factor 4 (TCF4) gene. Repeat-associated non-ATG (RAN) translation, an unconventional protein translation mechanism that does not require an initiating ATG, has been described in many TNR expansion diseases, including myotonic dystrophy type 1 (DM1). Given the similarities between DM1 and FECD, we wished to determine whether RAN translation occurs in FECD...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677157/molecular-characterization-and-the-function-of-argonaute3-in-rnai-pathway-of-plutella-xylostella
#16
Muhammad Salman Hameed, Zhengbing Wang, Liette Vasseur, Guang Yang
Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella ( PxAgo3 ) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain...
April 20, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29676727/characterization-of-sccmec-type-iv-methicillin-resistant-staphylococcus-aureus-clones-increased-in-japanese-hospitals
#17
Hidemasa Nakaminami, Shunsuke Takadama, Ayumu Ito, Mariko Hasegawa, Chika Jono, Miyuki Noguchi, Manami Shoshi, Takeaki Wajima, Takeshi Fujii, Hiroshi Maruyama, Haruo Sakamoto, Yutaka Ito, Satsuki Okamoto, Yukiyoshi Masaki, Kayoko Tsuchiya, Susumu Nishinarita, Norihisa Noguchi
Recently, the prevalence of staphylococcal cassette chromosome mec (SCCmec) type IV isolates, which are the major community-acquired methicillin-resistant Staphylococcus aureus (MRSA), have increased in Japanese hospitals. The aim of this study was to elucidate the detailed molecular epidemiological features of the SCCmec type IV clones in Japanese hospitals. When 2589 MRSA isolated from four hospitals in Tokyo, Japan between 2010 and 2014 were analysed, the proportion of SCCmec type IV overtook that of type II, which was the major type of hospital-acquired MRSA in 2014...
April 20, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29676515/characterization-of-a-novel-allelic-variant-in-hla-b-40-lineage-hla-b-40-298-02-by-cloning-and-sequencing
#18
W Tian, F M Zhu, W Y Wang, L X Li, J H Cai
A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction-Sanger sequence-based typing (PCR-SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule...
April 20, 2018: International Journal of Immunogenetics
https://www.readbyqxmd.com/read/29676149/biosynthesis-of-polyunsaturated-fatty-acids-in-the-razor-clam-sinonovacula-constricta-characterization-of-%C3%AE-5-and-%C3%AE-6-fatty-acid-desaturases
#19
Zhaoshou Ran, Jilin Xu, Kai Liao, Shuang Li, Shubing Chen, Xiaojun Yan
To investigate the endogenous LC-PUFA biosynthetic ability in Sinonovacula constricta, fatty acid desaturases (Fads) of this bivalve were cloned and characterized in current study, namely Scfad5a, Scfad5b and Scfad6. Meanwhile, the tissue distributions of S. constricta Fads and fatty acids (FAs) were examined. Heterologous expression in yeasts confirmed that Scfad5a and Scfad5b were both Δ5 Fads, while Scfad6 was a Δ6 Fad. However, compared with Fads in other organisms, the desaturation activities of S. constricta Fads were relatively low (especially for Scfad6), indicating an adaptation to living conditions...
April 20, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29674657/emergence-of-vancomycin-resistant-enterococcus-faecium-at-an-australian-hospital-a-whole-genome-sequencing-analysis
#20
Kelvin W C Leong, Louise A Cooley, Tara L Anderson, Sanjay S Gautam, Belinda McEwan, Anne Wells, Fiona Wilson, Lucy Hughson, Ronan F O'Toole
In 2015, a marked increase in vancomycin-resistant Enterococcus faecium (VREfm) isolation was detected at the Royal Hobart Hospital, Australia. The primary objective of this work was to examine the dynamics of VREfm transmission using whole genome data mapped to public health surveillance information. Screening and clinical isolates of VREfm from patients were typed for the specific vancomycin-resistance locus present. Of total isolates collected from 2014-2016 (n = 222), 15.3% and 84.7% harboured either the vanA or the vanB vancomycin-resistance locus, respectively...
April 19, 2018: Scientific Reports
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