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https://www.readbyqxmd.com/read/29244739/metabolic-characterization-of-the-anthocyanidin-reductase-pathway-involved-in-the-biosynthesis-of-flavan-3-ols-in-elite-shuchazao-tea-camellia-sinensis-cultivar-in-the-field
#1
Lei Zhao, Xiao-Lan Jiang, Yu-Mei Qian, Pei-Qiang Wang, De-Yu Xie, Li-Ping Gao, Tao Xia
Anthocyanidin reductase (ANR) is a key enzyme in the ANR biosynthetic pathway of flavan-3-ols and proanthocyanidins (PAs) in plants. Herein, we report characterization of the ANR pathway of flavan-3-ols in Shuchazao tea (Camellia sinesis), which is an elite and widely grown cultivar in China and is rich in flavan-3-ols providing with high nutritional value to human health. In our study, metabolic profiling was preformed to identify two conjugates and four aglycones of flavan-3-ols: (-)-epigallocatechin-gallate [(-)-EGCG], (-)-epicatechin-gallate [(-)-ECG], (-)-epigallocatechin [(-)-EGC], (-)-epicatechin [(-)-EC], (+)-catechin [(+)-Ca], and (+)-gallocatechin [(+)-GC], of which (-)-EGCG, (-)-ECG, (-)-EGC, and (-)-EC accounted for 70-85% of total flavan-3-ols in different tissues...
December 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29244249/flow-cytometric-false-myeloperoxidase-positive-childhood-b-lineage-acute-lymphoblastic-leukemia
#2
Süreyya Savaşan, Steven Buck, Manisha Gadgeel, Ali Gabali
BACKGROUND: Flow cytometric intracellular myeloperoxidase (MPO) staining of leukemic blasts is a useful tool in diagnosis of leukemia subtype. Interpretation of high MPO-positivity can be a diagnostic challenge in B-lineage acute lymphoblastic leukemia (B-ALL). While very few such cases have been reported, high MPO positive B-ALL cases without additional myeloid antigen positivity are suspect and require further investigation. METHODS: Three pediatric cases of B-ALL with strong MPO staining (clone 8E6; Invitrogen) at diagnosis and three others with negative MPO staining were studied by flow cytometry and immunohistochemistry...
December 15, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29244184/accumulation-of-long-term-transcriptionally-active-integrated-retroviral-vectors-in-active-promoters-and-enhancers
#3
Filip Šenigl, Dalibor Miklík, Miroslav Auxt, Jirí Hejnar
Most retroviruses preferentially integrate into certain genomic locations and, as a result, their genome-wide integration patterns are non-random. We investigate the epigenetic landscape of integrated retroviral vectors and correlate it with the long-term stability of proviral transcription. Retroviral vectors derived from the avian sarcoma/leukosis virus expressing the GFP reporter were used to transduce the human myeloid lymphoblastoma cell line K562. Because of efficient silencing of avian retrovirus in mammalian cells, only ∼3% of established clones displayed stable proviral expression...
December 15, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29244145/biological-and-therapeutic-implications-of-multisector-sequencing-in-newly-diagnosed-glioblastomas
#4
Tatenda Mahlokozera, Ananth K Vellimana, Tiandao Li, Diane D Mao, Zohny S Zohny, David H Kim, David D Tran, Daniel S Marcus, Sarah J Fouke, Jian L Campian, Gavin P Dunn, Christopher A Miller, Albert H Kim
Background: Diagnostic workflows for glioblastoma (GBM) patients increasingly include DNA sequencing-based analysis of a single tumor site following biopsy or resection. We hypothesized that sequencing of multiple sectors within a given tumor would provide a more comprehensive representation of the molecular landscape and potentially inform therapeutic strategies. Methods: 10 newly diagnosed, IDH1 wildtype GBM tumor samples were obtained from two (n = 9) or four (n = 1) spatially distinct tumor regions...
December 13, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29243837/a-minireplicon-of-plasmid-pbmb26-represents-a-new-typical-replicon-in-the-megaplasmids-of-bacillus-cereus-group
#5
Pengxia Wang, Yiguang Zhu, Hui Shang, Yun Deng, Ming Sun
A new minireplicon (rep26 minireplicon) from pBMB26, the 188 kb indigenous plasmid related to spore-crystal association (SCA) phenotype in Bacillus thuringiensis strain YBT-020, was characterized. A 12 kb EcoRI fragment, which encoded 10 putative open reading frames (ORFs), was capable of supporting replication when cloned in a replication probe vector. Deletion and frame shift mutation analysis showed that a 4.1 kb region encompassing two putative ORFs (orf21 and orf22) was essential for the plasmid replication in B...
December 15, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29243778/apatinib-resensitizes-cisplatin-resistant-non-small-cell-lung-carcinoma-a549-cell-through-reversing-multidrug-resistance-and-suppressing-erk-signaling-pathway
#6
Z-L Liu, B-J Jin, C-G Cheng, F-X Zhang, S-W Wang, Y Wang, B Wu
OBJECTIVE: To observe the reversal effect of apatinib on the resistance to cisplatin (DDP) of A549/cisplatin (A549/DDP) cells and its relevant mechanism. MATERIALS AND METHODS: A549/DDP cells were treated with the control method, apatinib alone, DDP alone and DDP combined with apatinib. The cell proliferation was detected by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the cell clone formation assay. The cell apoptosis was detected by Hoechst 33258 staining and annexin V and propidium iodide (PI) double labeling...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29243775/independent-prognostic-factor-of-low-expressed-lncrna-znf667-as1-for-cervical-cancer-and-inhibitory-function-on-the-proliferation-of-cervical-cancer
#7
L-P Zhao, R-H Li, D-M Han, X-Q Zhang, G-X Nian, M-X Wu, Y Feng, L Zhang, Z-G Sun
OBJECTIVE: To investigate the expression of long non-coding RNA ZNF667-AS1 in cervical cancer and its effect on the proliferation of cervical cancer cell line, SiHa cells. MATERIALS AND METHODS: The expression level of ZNF667-AS1 from two microarray datasets (GSE63514 and GSE6791) and TCGA (The Cancer Genome Atlas) were selected to analyze the difference between cervical cancer tissues and normal cervical tissues with bioinformatics methods. Then, the prognosis of ZNF667-AS1 was calculated in TCGA...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29243423/-effects-of-sampling-time-on-polysaccharides-of-dendrobium-catenatum-under-temperature-controlled-condition
#8
Qi Wang, Jing-Jing Liu, Jin-Ping Si, Yu-Qiu Zhu, Lang Qin, Ju-Ling Zhang
In order to reveal the accumulation trend of polysaccharides in Dendrobium catenatum and determine the effect of sampling time on polysaccharides, D. Catenatum D21 clone was harvested from January to December after culturing for 2 to 5 months in the growth chamber with constant temperature. Polysaccharides were determined by phenol-sulfuric acid method and the monosaccharide compositions were analyzed by pre-column derivative-UPLC. The results showed that the content of polysaccharide and its key component mannose was positively correlated with the culture time, but the contents of polysaccharides in all kinds of culture peaked from 5 to 6 months, which were consistent with the trend of field planting...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29243215/identification-of-banana-lectin-isoforms-and-differential-acetylation-through-mass-spectrometry-approaches
#9
B S Gnanesh Kumar, Avadhesha Surolia
Banana lectin (BanLec) exhibits specificity to glucose/mannose residues present in oligo saccharides or glycoconjugates and has attracted a lot of attention recently as a potent therapeutic agent. Structural studies and molecular cloning methods has revealed the presence of three different BanLec proteins in two species. In our study, initial mass spectrometric analysis of affinity purified native BanLec from banana pulp (Musa paradisiaca) indicated the presence of proteins with different molecular mass. Through the bottom up and top down analysis we identified three major isoforms with acetylation at N terminus...
December 14, 2017: Protein Journal
https://www.readbyqxmd.com/read/29242829/molecular-mechanisms-for-microbe-recognition-and-defense-by-the-red-seaweed-laurencia-dendroidea
#10
Louisi Souza de Oliveira, Diogo Antonio Tschoeke, Ana Carolina Rubem Magalhães Lopes, Daniela Bueno Sudatti, Pedro Milet Meirelles, Cristiane C Thompson, Renato Crespo Pereira, Fabiano L Thompson
The ability to recognize and respond to the presence of microbes is an essential strategy for seaweeds to survive in the marine environment, but understanding of molecular seaweed-microbe interactions is limited. Laurencia dendroidea clones were inoculated with the marine bacterium Vibrio madracius. The seaweed RNA was sequenced, providing an unprecedentedly high coverage of the transcriptome of Laurencia, and the gene expression levels were compared between control and inoculated samples after 24, 48, and 72 h...
November 2017: MSphere
https://www.readbyqxmd.com/read/29242607/the-impact-of-stromal-hic-5-on-the-tumorigenesis-of-colorectal-cancer-through-lysyl-oxidase-induction-and-stromal-remodeling
#11
Tomokatsu Omoto, Joo-Ri Kim-Kaneyama, Xiao-Feng Lei, Akira Orimo, Koji Ohnishi, Kosuke Yoshihara, Aya Miyauchi, Shuo Li, Lin Gao, Takahiro Umemoto, Junichi Tanaka, Kenta Nakahara, Motohiro Takeya, Fumio Ishida, Shin-Ei Kudo, Shogo Haraguchi, Takuro Miyazaki, Akira Miyazaki
Carcinoma-associated fibroblasts (CAFs) influence tumor initiation, progression, and metastasis within the tumor-associated stroma. This suggests that CAFs would be a potential target for tumor therapy. Here we found that Hydrogen peroxide-inducible clone-5 (Hic-5), also named transforming growth factor beta-1-induced transcript 1 protein (Tgfb1i1), was strongly induced in CAFs found in human colorectal cancer. To investigate the role of Hic-5 in CAFs, we isolated CAFs and the control counterpart normal fibroblasts (NFs) from human colorectal cancer and non-cancerous regions, respectively...
December 15, 2017: Oncogene
https://www.readbyqxmd.com/read/29242287/the-local-co-existence-pattern-of-selfing-genotypes-in-caenorhabditis-elegans-natural-metapopulations
#12
Aurélien Richaud, Gaotian Zhang, Daehan Lee, Junho Lee, Marie-Anne Félix
To study the interplay of rare outcrossing and metapopulation structure, we focus on the nematode Caenorhabditis elegans Its remarkably low outcrossing rate is at the extreme end of the spectrum for facultative selfing organisms. At the demographic level, C. elegans natural populations undergo boom and bust dynamics on ephemeral resources, the dauer diapause larva acting as the dispersal form. Here we investigate the small-scale genetic structure of C. elegans populations in two localities over several years, using 2b-RAD (Restriction-Associated DNA) sequencing of nearly a thousand individuals...
December 13, 2017: Genetics
https://www.readbyqxmd.com/read/29242186/biased-igh-vdj-gene-repertoire-and-clonal-expansions-in-b-cells-of-chronically-hepatitis-c-virus-infected-individuals
#13
Felicia A Tucci, Simo Kitanovski, Patricia Johansson, Ludger Klein-Hitpass, Alisan Kahraman, Jan Dürig, Daniel Hoffmann, Ralf Küppers
Patients chronically infected with hepatitis C virus (HCV) frequently develop mixed cryoglobulinemia (MC), a monoclonal expansion of IgM+ autoreactive B cells, and also have an increased B cell lymphoma risk. Whether HCV infection also impacts the B cell compartment and the B cell receptor repertoire in patients not affected by MC or lymphomas is poorly understood. Flow cytometric analysis of peripheral blood B cells of 30 MC-negative HCV-infected patients and 15 healthy controls revealed that frequencies of class-switched memory B cells were increased in the patients, whereas frequencies of transitional and naive B cells were decreased...
December 14, 2017: Blood
https://www.readbyqxmd.com/read/29242148/whole-bacterium-selex-of-dna-aptamers-for-rapid-detection-of-e-coli-o157-h7-using-a-qcm-sensor
#14
Xiaofan Yu, Fang Chen, Ronghui Wang, Yanbin Li
The rapid detection of foodborne pathogens is critical to ensure food safety. The objective of this study is to select aptamers specifically bound to Escherichia coli O157:H7 using the whole-bacterium SELEX (Systematic Evolution of Ligands by Exponential Enrichment) and apply the selected aptamer to a QCM (quartz crystal microbalance) sensor for rapid and sensitive detection of target bacteria. A total of 19 rounds of selection against live E. coli O157:H7 and 6 rounds of counter selection against a mixture of Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhimurium, were performed...
December 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29242132/molecular-characterization-expression-and-functional-analysis-of-nod1-nod2-and-nlrc3-in-nile-tilapia-oreochromis-niloticus
#15
Feng-Ying Gao, Ji-Cai Pang, Mai-Xin Lu, Xian-le Yang, Hua-Ping Zhu, Xiao-Li Ke, Zhi-Gang Liu, Jian-Meng Cao, Miao Wang
The nucleotide-binding oligomerization domain proteins NOD1, NOD2 and NLRC3 are cytoplasmic pattern recognition receptors (PRRs) of the Nod-like receptor (NLR) family. In the present study, the Nile tilapia (Oreochromis niloticus) NOD1 (ntNOD1), NOD2 (ntNOD2) and NLRC3 (ntNLRC3) genes were cloned and characterized. The full-length ntNOD1, ntNOD2 and ntNLRC3 genes were 3924, 3886 and 4574 bp, encoding 941, 986 and 1130 amino acids, respectively. The three Nod-like receptors have a NACHT domain and a C-terminal leucine-rich repeat (LRR) domain...
December 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29242049/rational-library-design-by-functional-cdr-resampling
#16
Qi Zhao, Diane Buhr, Courtney Gunter, Jenny Frenette, Mary Ferguson, Eric Sanford, Erika Holland, Chitra Rajagopal, Melissa Batonick, Margaret M Kiss, Michael P Weiner
Successful antibody discovery relies on diversified libraries, where two aspects are implied, namely the absolute number of unique clones and the percentage of functional clones. Instead of pursuing the absolute quantity thresholded by current display technology, we have sought to maximize the effective diversity by improving functional clone percentage. With the combined effort of bioinformatics, structural biology, molecular immunology and phage display technology, we devised a bioinformatic pipeline to construct and validate libraries via combinatorial assembly of sequences from a database of experimentally validated antibodies...
December 11, 2017: New Biotechnology
https://www.readbyqxmd.com/read/29241570/zmnst3-and-zmnst4-are-master-switches-for-secondary-wall-deposition-in-maize-zea-mays-l
#17
Wenhan Xiao, Yue Yang, Jingjuan Yu
Secondary walls are the most abundant biomass produced by plants, and they consist mainly of lignin, cellulose and hemicellulose. Understanding how secondary wall biosynthesis is regulated could potentially provide genetic tools for engineering biomass components, especially in maize and Sorghum bicolor. Although many works have focused on secondary wall biosynthesis in dicotyledons, little has been reported for these monocotyledons. In this study, we cloned two NAC transcriptional factor genes, ZmNST3 and ZmNST4, and analyzed their functions in maize secondary wall formation process...
January 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29240926/exocet-exonuclease-in-vitro-assembly-combined-with-recet-recombination-for-highly-efficient-direct-dna-cloning-from-complex-genomes
#18
Hailong Wang, Zhen Li, Ruonan Jia, Jia Yin, Aiying Li, Liqiu Xia, Yulong Yin, Rolf Müller, Jun Fu, A Francis Stewart, Youming Zhang
The exponentially increasing volumes of DNA sequence data highlight the need for new DNA cloning methods to explore the new information. Here, we describe 'ExoCET' (Exonuclease Combined with RecET recombination) to directly clone any chosen region from bacterial and mammalian genomes with nucleotide precision into operational plasmids. ExoCET combines in vitro exonuclease and annealing with the remarkable capacity of full length RecET homologous recombination (HR) to retrieve specified regions from genomic DNA preparations...
December 12, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29240834/discovery-of-carbamate-degrading-enzymes-by-functional-metagenomics
#19
Lisa Ufarté, Elisabeth Laville, Sophie Duquesne, Diego Morgavi, Patrick Robe, Christophe Klopp, Angeline Rizzo, Sandra Pizzut-Serin, Gabrielle Potocki-Veronese
Bioremediation of pollutants is a major concern worldwide, leading to the research of new processes to break down and recycle xenobiotics and environment contaminating polymers. Among them, carbamates have a very broad spectrum of uses, such as toxinogenic pesticides or elastomers. In this study, we mined the bovine rumen microbiome for carbamate degrading enzymes. We isolated 26 hit clones exhibiting esterase activity, and were able to degrade at least one of the targeted polyurethane and pesticide carbamate compounds...
2017: PloS One
https://www.readbyqxmd.com/read/29240831/comparative-transcriptome-profiling-of-virulent-and-non-virulent-trypanosoma-cruzi-underlines-the-role-of-surface-proteins-during-infection
#20
A Trey Belew, Caroline Junqueira, Gabriela F Rodrigues-Luiz, Bruna M Valente, Antonio Edson R Oliveira, Rafael B Polidoro, Luciana W Zuccherato, Daniella C Bartholomeu, Sergio Schenkman, Ricardo T Gazzinelli, Barbara A Burleigh, Najib M El-Sayed, Santuza M R Teixeira
Trypanosoma cruzi, the protozoan that causes Chagas disease, has a complex life cycle involving several morphologically and biochemically distinct stages that establish intricate interactions with various insect and mammalian hosts. It has also a heterogeneous population structure comprising strains with distinct properties such as virulence, sensitivity to drugs, antigenic profile and tissue tropism. We present a comparative transcriptome analysis of two cloned T. cruzi strains that display contrasting virulence phenotypes in animal models of infection: CL Brener is a virulent clone and CL-14 is a clone that is neither infective nor pathogenic in in vivo models of infection...
December 14, 2017: PLoS Pathogens
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