keyword
MENU ▼
Read by QxMD icon Read
search

Cloning

keyword
https://www.readbyqxmd.com/read/29458065/nosocomial-ventriculitis-caused-by-a-meticillin-and-linezolid-resistant-clone-of-staphylococcus-epidermidis-in-neurosurgical-patients
#1
Carlos Rodríguez-Lucas, Javier Fernández, José Antonio Boga, Lucia López-Amor, Lorena Forcelledo, Eva Lázaro-López, M Rosario Rodicio
BACKGROUND: Post-neurosurgical ventriculitis is mainly caused by coagulase-negative staphylococci. The rate of linezolid resistant S. epidermidis (LRSE) is increasing worldwide. AIMS: To report clinical and microbiological data from a series of ventriculitis cases caused by LRSE in a Spanish hospital between 2013 and 2016. METHODS: LRSE ventriculitis cases were retrospectively reviewed in a Spanish hospital during a four-year period. Clinical/epidemiological data of the infected patients were reviewed, the isolates involved were typed by PFGE and MLST, and the molecular basis of linezolid resistance was determined...
February 16, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29457642/identification-and-characterization-of-neutralization-epitopes-at-vp2-and-vp1-of-enterovirus-a71
#2
Nmn NikNadia, Chee Wah Tan, Kien Chai Ong, I-Ching Sam, Yoke Fun Chan
Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection...
February 19, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29457321/wine-aromatic-compound-production-and-fermentative-behavior-within-different-non-saccharomyces-species-and-clones
#3
Rocío Escribano, Lucía González-Arenzana, Javier Portu, Patrocinio Garijo, Isabel López-Alfaro, Rosa López, Pilar Santamaría, Ana Rosa Gutiérrez
AIMS: 25 enological yeasts belonging to 9 different species (Candida zeylanoides, Cryptococcus uzbekistanensis, Debaryomyces hansenii, Lachancea thermotolerans, Metschnikowia pulcherrima, Torulaspora delbrueckii, Williopsis pratensis, Zygosaccharomyces bailii and Saccharomyces cerevisiae) were screened for aroma formation and fermentative behavior as part of a non-Saccharomyces yeasts selection program. METHODS AND RESULTS: Pure cultures were inoculated in pasteurized grape juice in order to perform alcoholic fermentations...
February 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29456971/molecular-biochemical-and-kinetic-analysis-of-a-novel-thermostable-lipase-lipsm-from-stenotrophomonas-maltophilia-psi-1-the-first-member-of-a-new-bacterial-lipase-family-xviii
#4
Maria Parapouli, Athanasios Foukis, Panagiota-Yiolanda Stergiou, Maria Koukouritaki, Panagiotis Magklaras, Olga A Gkini, Emmanuel M Papamichael, Amalia-Sofia Afendra, Efstathios Hatziloukas
Background: Microbial lipases catalyze a broad spectrum of reactions and are enzymes of considerable biotechnological interest. The focus of this study was the isolation of new lipase genes, intending to discover novel lipases whose products bear interesting biochemical and structural features and may have a potential to act as valuable biocatalysts in industrial applications. Results: A novel lipase gene ( lipSm ), from a new environmental Stenotrophomonas maltophilia strain, Psi-1, originating from a sludge sample from Psittaleia (Greece), was cloned and sequenced...
December 2018: Journal of Biological Research
https://www.readbyqxmd.com/read/29456867/heterologous-and-endogenous-u6-snrna-promoters-enable-crispr-cas9-mediated-genome-editing-in-aspergillus-niger
#5
Xiaomei Zheng, Ping Zheng, Jibin Sun, Zhang Kun, Yanhe Ma
Background: U6 promoters have been used for single guide RNA (sgRNA) transcription in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas9) genome editing system. However, no available U6 promoters have been identified in Aspergillus niger, which is an important industrial platform for organic acid and protein production. Two CRISPR/Cas9 systems established in A. niger have recourse to the RNA polymerase II promoter or in vitro transcription for sgRNA synthesis, but these approaches generally increase cloning efforts and genetic manipulation...
2018: Fungal Biology and Biotechnology
https://www.readbyqxmd.com/read/29456820/immunogenicity-of-heparin-binding-hemagglutinin-expressed-by-pichia-pastoris-gs115-strain
#6
Xindong Teng, Xiaoguang Chen, Ke Zhu, Hefei Xu
Objectives: Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis ( M. tuberculosis ) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins for the purpose of diagnosing M. tuberculosis infection or for the immunization. Here, HBHA was expressed by Pichia pastoris ( P. pastoris ) GS115 strain , and the immunogenicity of HBHA was evaluated...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29456773/molecular-characterization-of-multidrug-resistant-uropathogenic-e-coli-isolates-from-jordanian-patients
#7
Yacoub R Nairoukh, Azmi M Mahafzah, Amal Irshaid, Asem A Shehabi
Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29456736/-brucea-javanica-seed-oil-enhances-the-radiosensitivity-of-esophageal-cancer-by-inhibiting-hypoxia-inducible-factor-1%C3%AE-in-vitro-and-in-vivo
#8
Peng Pan, Bai-Xia Yang, Xiao-Lin Ge
Brucea javanica oil emulsion (BJOE) has been used clinically to treat esophageal cancer combined with radiotherapy for numerous years in China. However, the detailed mechanism remains unclear. Thus, the effects of BJOE on the radiosensitivity of esophageal squamous cell carcinoma (ESCC) were evaluated in vitro and in vivo . The growth inhibitory effects of different BJOE concentrations were determined through an MTT assay. Radiosensitivity was evaluated through focal formation measurements and clone formation assays...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456651/protective-effect-of-black-garlic-extracts-on-tert-butyl-hydroperoxide-induced-injury-in-hepatocytes-via-a-c-jun-n-terminal-kinase-dependent-mechanism
#9
Ko-Chao Lee, Chih-Chuan Teng, Chien-Heng Shen, Wen-Shih Huang, Chien-Chang Lu, Hsing-Chun Kuo, Shui-Yi Tung
Black garlic has been reported to show multiple bioactivities against the development of different diseases. In the present study, the hepatoprotective effect of black garlic on injured liver cells was investigated. Rat clone-9 hepatocytes were used for all experiments; tert-Butyl hydroperoxide (tBHP) was used to induce injury of rat clone-9 hepatocytes. The contents of malondialdehyde (MDA) and glutathione (GSH); anti-oxidative enzyme activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx); and mRNA expression levels of interleukin (IL)-6 and IL-8 in rat clone-9 hepatocytes were determined to evaluate the level of cell damage...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456350/recurrent-glioblastomas-exhibit-higher-expression-of-biomarkers-with-stem-like-properties
#10
B N Nandeesh, Sharmistha Naskar, Arun H Shashtri, A Arivazhagan, Vani Santosh
Background: Despite advances in the treatment of glioblastoma (GBM), the prognosis of patients continues to remain dismal. This unfavorable prognosis is mainly attributed to the tumor's propensity for progression and recurrence, which in turn is due to the highly aggressive nature of the persisting GBM cells that actively egress from the main tumor mass into the surrounding normal brain tissue. Such a recurrent tumor described to have a more malignant potential is highly invasive and resistant to current therapies, probably due to increased stemness and preferential selection of therapy-resistant clones of tumor cells...
January 2018: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/29455735/corrigendum-to-flow-cytometric-quantitation-of-platelet-phagocytosis-by-monocytes-using-a-ph-sensitive-dye-phrodo-se-journal-of-immunological-methods-447-2017-57-64
#11
Daisuke Takahashi, Mitsuhiro Fujihara, Toru Miyazaki, Keiji Matsubayashi, Shinichiro Sato, Hiroshi Azuma, Toshiaki Kato, Shuichi Kino, Hisami Ikeda, Shigeru Takamoto, Noriyuki Sato, Toshihiko Torigoe
Antibody-mediated phagocytosis of platelets using a flow cytometric monocyte-based phagocytosis assay (FMPA) has been shown to predict the outcome of platelet transfusion. The easy adherence between platelets and monocytes even in the absence of an antibody is regarded as one of limitations of the FMPA. To improve the FMPA for prediction of transfusion outcome, we used the pH-sensitive dye pHrodo succinimidyl ester (pHrodo-SE), which has weak fluorescence at neutral pH and has increased fluorescence intensity in low pH conditions such as in lysomes...
March 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29455388/heterologous-expression-of-rtshyal-1-the-first-recombinant-hyaluronidase-of-scorpion-venom-produced-in-pichia-pastoris-system
#12
Fernanda Gobbi Amorim, Johara Boldrini-França, Karla de Castro Figueiredo Bordon, Iara Aimê Cardoso, Edwin De Pauw, Loïc Quinton, Simone Kashima, Eliane Candiani Arantes
In general, hyaluronidases have a broad potential application on medicine and esthetics fields. Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim. However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation. Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies...
February 17, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29455192/cloning-and-expression-analysis-of-cdnas-encoding-adp-glucose-pyrophosphorylase-large-and-small-subunits-from-hulless-barley-hordeum-vulgare-l-var-nudum
#13
Dongmei Li, Zhimin Yang, Xinchun Liu, Zhen Song, Zongyun Feng, Yang He
As an important plateau cereal crop, hulless barley is the principal food for the Tibetan people in China. ADP-glucose pyrophosphorylase (AGPase) is considered as the key enzyme for starch biosynthesis in plants. In this study, cDNAs encoding the small subunit (SSU I) and large subunit (LSU I) of AGPase were isolated from hulless barley. The results showed that SSU I and LSU I were 1438 and 1786 bp in length with a complete open reading frame (ORF) of 1419 and 1572 bp. The ORF-encoded polypeptides of 472 and 523 amino acids were having calculated molecular masses of 52...
February 19, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29455065/foot-and-mouth-disease-virus-type-o-specific-mutations-determine-rna-dependent-rna-polymerase-fidelity-and-virus-attenuation
#14
Chen Li, Haiwei Wang, Tiangang Yuan, Andrew Woodman, Decheng Yang, Guohui Zhou, Craig E Cameron, Li Yu
Previous studies have shown that the FMDV Asia1/YS/CHA/05 high-fidelity mutagen-resistant variants are attenuated (Zeng et al., 2014). Here, we introduced the same single or multiple-amino-acid substitutions responsible for increased 3Dpol fidelity of type Asia1 FMDV into the type O FMDV O/YS/CHA/05 infectious clone. The rescued viruses O-DA and O-DAMM are lower replication fidelity mutants and showed an attenuated phenotype. These results demonstrated that the same amino acid substitution of 3Dpol in different serotypes of FMDV strains had different effects on viral fidelity...
February 15, 2018: Virology
https://www.readbyqxmd.com/read/29454942/cloning-molecular-modeling-and-characterization-of-acidic-cellulase-from-buffalo-rumen-and-its-applicability-in-saccharification-of-lignocellulosic-biomass
#15
Tripti Dadheech, Ravi Shah, Ramesh Pandit, Ankit Hinsu, Prakram Singh Chauhan, Subhash Jakhesara, Anju Kunjadiya, Dharamshibhai Rank, Chaitanya Joshi
Cellulase hydrolyses the cellulose by cleaving the β-1,4-linkages to produce mono-, oligo- and shorter polysaccharide units. These enzymes have applications in various industries such as pulp and paper, laundry, food and feed, textile, brewing industry and in biofuel production. In the present study we have cloned acid-cellulase gene (Cel-1) from the fosmid library of buffalo rumen metagenomic DNA and functionally expressed it in Escherichia coli. The ORF encoding cellulase consisted of 1176-bp, corresponding to protein of 391 amino acid and has catalytic domain belonging to glycosyl hydrolase family 5...
February 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29454897/involvement-of-eif2%C3%AE-of-epinephelus-coioides-in-the-fish-immune-response-to-virus-infection
#16
Shaoqing Zang, Xin Zhang, Jingcheng Zhang, Chen Li, Jingguang Wei, Qiwei Qin
The eukaryotic initiation factor 2 alpha subunit (eIF2α) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2α homolog (EceIF2α) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EceIF2α encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with eIF2αs from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EceIF2α was distributed in all examined tissues...
February 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29454850/dissemination-of-panton-valentine-leukocidin-positive-methicillin-resistant-staphylococcus-aureus-usa300-clone-in-multiple-hospitals-in-tokyo-japan
#17
Shunsuke Takadama, Hidemasa Nakaminami, Asami Sato, Manami Shoshi, Takeshi Fujii, Norihisa Noguchi
OBJECTIVE: Panton-Valentine leukocidin (PVL) gene (lukS/F-PV)-positive community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA), particularly the USA300 clone, is a health concern worldwide. Recently, CA-MRSA clones have been found to spread and persist in hospital settings. In this study, we aimed to investigate the prevalence and molecular epidemiological features of lukS/F-PV-positive MRSA in Japanese hospitals. METHODS: A total of 3,433 MRSA isolated from nine hospitals in 2011-2015 were used...
February 15, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29454832/identification-and-functional-analysis-of-inhibitor-of-nf-%C3%AE%C2%BAb-kinase-ikk-from-scylla-paramamosain-the-first-evidence-of-three-ikks-in-crab-species-and-their-expression-profiles-under-biotic-and-abiotic-stresses
#18
Mei Jiang, Dan-Dan Tu, Wen-Bin Gu, Yi-Lian Zhou, Qi-Hui Zhu, Xiao-Ling Guo, Miao-An Shu
IKK (inhibitor of NF-κB kinase) is the core regulator for NF-κB (nuclear factor-κB) pathway against pathogenic invasion in vertebrates or invertebrates. However, the IKK from crab species has not yet been identified. In the present study, three full-length cDNA sequences of IKKs from mud crab Scylla paramamosain, designated as SpIKKβ, SpIKKε1 and SpIKKε2, were firstly cloned through RT-PCR and RACE methods. This is also the first report about the identification of two IKKε genes in mud crab and even in crustaceans...
February 15, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29454794/hcv-genotype-6a-escape-from-and-resistance-to-velpatasvir-pibrentasvir-and-sofosbuvir-in-robust-infectious-cell-culture-models
#19
Long V Pham, Santseharay Ramirez, Judith M Gottwein, Ulrik Fahnøe, Yi-Ping Li, Jannie Pedersen, Jens Bukh
BACKGROUND & AIMS: Chronic liver diseases caused by hepatitis C virus (HCV) genotype 6 are prevalent in Asia, and millions of people require treatment with direct-acting antiviral regimens, such as NS5A inhibitor velpatasvir combined with the NS5B polymerase inhibitor sofosbuvir. We developed infectious cell culture models of HCV genotype 6a infection to study the effects of these inhibitors and the development of resistance. METHODS: The consensus sequences of prototype strains HK2 (MG717925) and HK6a (MG717928), originating from serum of patients with chronic HCV infection, were determined by Sanger sequencing of genomes amplified by reverse transcription-PCR...
February 15, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29454668/high-level-production-of-a-recombinant-acid-stable-exoinulinase-from-aspergillus-kawachii
#20
Mariana Chesini, Evelyn Wagner, Diego J Baruque, Carolina E Vita, Sebastián F Cavalitto, Pablo D Ghiringhelli, Natalia L Rojas
Exoinulinases-enzymes extensively studied in recent decades because of their industrial applications-need to be produced in suitable quantities in order to meet production demands. We describe here the production of an acid-stable recombinant inulinase from Aspergillus kawachii in the Pichia pastoris system and the recombinant enzyme's biochemical characteristics and potential application to industrial processes. After an appropriate cloning strategy, this genetically engineered inulinase was successfully overproduced in fed-batch fermentations, reaching up to 840 U/ml after a 72-h cultivation...
February 15, 2018: Protein Expression and Purification
keyword
keyword
42231
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"