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hemolysis biomarkers

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https://www.readbyqxmd.com/read/27814292/hemorheological-alterations-in-sickle-cell-anemia-and-their-clinical-consequences-the-role-of-genetic-modulators
#1
Marisa Silva, Sofia Vargas, Andreia Coelho, Alexandra Dias, Teresa Ferreira, Anabela Morais, Raquel Maia, Paula Kjöllerström, João Lavinha, Paula Faustino
Sickle cell anemia (SCA) is an autosomal recessive disease caused by the HBB:c.20A>T mutation that leads to hemoglobin S synthesis. The disease presents with high clinical heterogeneity characterized by chronic hemolysis, recurrent episodes of vaso-oclusion and infection. This work aimed to characterize by in silico studies some genetic modulators of severe hemolysis and stroke risk in children with SCA, and understand their consequences at the hemorheological level.Association studies were performed between hemolysis biomarkers as well as the degree of cerebral vasculopathy and the inheritance of several polymorphic regions in genes related with vascular cell adhesion and vascular tonus in pediatric SCA patients...
November 4, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27667587/expression-pattern-of-cd55-and-cd59-on-red-blood-cells-in-sickle-cell-disease
#2
Lama Al-Faris, Monera Al-Rukhayes, Salah Al-Humood
OBJECTIVES: To investigate the pattern of CD55 and CD59 expression on RBCs of SCD patients, and its association with anemia, biochemical parameters of hemolysis, level of erythropoietin, and pro-inflammatory markers. METHODS: Flow cytometric analysis was performed on RBCs from 71 adult SCD patients and 53 healthy controls, using the commercial REDQUANT kit. RESULTS: CD59 deficiency was significantly higher among SCD patients than among healthy controls...
September 25, 2016: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/27644115/extravascular-hemolysis-and-complement-consumption-in-paroxysmal-nocturnal-hemoglobinuria-patients-undergoing-eculizumab-treatment
#3
Marta Subías Hidalgo, Hector Martin Merinero, Alicia López, Jaouad Anter, Sheila Pinto García, Fernando Ataúlfo Gonzalez-Fernández, Rafael Forés, Margarita Lopez-Trascasa, Ana Villegas, Emilio Ojeda, Santiago Rodríguez de Córdoba
Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hemolytic anemia characterized by complement-mediated intravascular hemolysis that is effectively treated with eculizumab. However, treatment responses are reported heterogeneous with some patients presenting residual hemolysis and requiring RBC transfusions. Recent reports have shown that both extravascular hemolysis and incomplete C5 blockade can explain these suboptimal hematological responses. Here we have tested our eculizumab-treated PNH patients (n=12) for signs of hemolysis and assessed complement biomarkers...
September 13, 2016: Immunobiology
https://www.readbyqxmd.com/read/27642014/ultra-acute-diagnostics-for-stroke-large-scale-implementation-of-prehospital-biomarker-sampling
#4
O S Mattila, H Harve, S Pihlasviita, J Ritvonen, G Sibolt, M Pystynen, D Strbian, S Curtze, M Kuisma, T Tatlisumak, P J Lindsberg
OBJECTIVES: Blood-based biomarkers could enable early and cost-effective diagnostics for acute stroke patients in the prehospital setting to support early initiation of treatments. To facilitate development of ultra-acute biomarkers, we set out to implement large-scale prehospital blood sampling and determine feasibility and diagnostic timesavings of this approach. MATERIALS AND METHODS: Emergency medical services (EMS) personnel of the Helsinki metropolitan area were trained to collect prehospital blood samples from thrombolysis candidates using a cannula adapter technique...
September 18, 2016: Acta Neurologica Scandinavica
https://www.readbyqxmd.com/read/27635308/specific-carotenoid-pigments-in-the-diet-and-a-bit-of-oxidative-stress-in-the-recipe-for-producing-red-carotenoid-based-signals
#5
Esther García-de Blas, Rafael Mateo, Carlos Alonso-Alvarez
Colorful ornaments have been the focus of sexual selection studies since the work of Darwin. Yellow to red coloration is often produced by carotenoid pigments. Different hypotheses have been formulated to explain the evolution of these traits as signals of individual quality. Many of these hypotheses involve the existence of a signal production cost. The carotenoids necessary for signaling can only be obtained from food. In this line, carotenoid-based signals could reveal an individual's capacity to find sufficient dietary pigments...
2016: PeerJ
https://www.readbyqxmd.com/read/27567707/a-mobile-phone-based-approach-to-detection-of-hemolysis
#6
Edikan Archibong, Karthik Raj Konnaiyan, Howard Kaplan, Anna Pyayt
Preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelet count) syndrome are pregnancy-related complications with high rates of morbidity and mortality. HELLP syndrome, in particular, can be difficult to diagnose. Recent work suggests that elevated levels of free cell hemoglobin in blood plasma can, as early as the first trimester, potentially serve as a diagnostic biomarker for impending complications. We therefore developed a point-of-care mobile phone-based platform that can quickly characterize a patient's level of hemolysis by measuring the color of blood plasma...
August 16, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27552216/determinants-of-carboxyhemoglobin-levels-and-relationship-with-sepsis-in-a-retrospective-cohort-of-preterm-neonates
#7
Andrew J McArdle, James Webbe, Kathleen Sim, Graham Parrish, Clive Hoggart, Yifei Wang, J Simon Kroll, Sunit Godambe, Aubrey J Cunnington
Carboxyhemoglobin levels in blood reflect endogenous carbon monoxide production and are often measured during routine blood gas analysis. Endogenous carbon monoxide production has been reported to be increased during sepsis, but carboxyhemoglobin levels have not been thoroughly evaluated as a biomarker of sepsis. We sought to determine whether carboxyhemoglobin levels were elevated during sepsis in a high risk population of premature neonates. We conducted a retrospective cohort study of 30 infants in two neonatal intensive care units using electronic medical and laboratory records...
2016: PloS One
https://www.readbyqxmd.com/read/27501106/detection-of-serum-micrornas-from-department-of-defense-serum-repository-correlation-with-cotinine-cytokine-and-polycyclic-aromatic-hydrocarbon-levels
#8
Collynn F Woeller, Thomas H Thatcher, Daniel Van Twisk, Stephen J Pollock, Amanda Croasdell, Nina Kim, Philip K Hopke, Xiaoyan Xia, Juilee Thakar, Col Timothy M Mallon, Mark J Utell, Richard P Phipps
OBJECTIVE: The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs). METHODS: MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy...
August 2016: Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/27429526/l-carnitine-as-a-potential-additive-in-blood-storage-solutions-a-study-on-erythrocytes
#9
R Soumya, H Carl, R Vani
Erythrocytes undergo various changes during storage (storage lesion) that in turn reduces their functioning and survival. Oxidative stress plays a major role in the storage lesion and antioxidants can be used to combat this stress. This study elucidates the effects of l-carnitine (LC) on erythrocytes of stored blood. Blood was obtained from male Wistar rats and stored (4 °C) for 20 days in CPDA-1 (citrate phosphate dextrose adenine) solution. Samples were divided into-(i) controls (ii) LC 10 (l-carnitine at a concentration of 10 mM) (iii) LC 30 (l-carnitine at a concentration of 30 mM) and (iv) LC 60 (l-carnitine at a concentration of 60 mM)...
September 2016: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/27373799/acute-kidney-injury-following-cardiac-surgery-current-understanding-and-future-directions
#10
REVIEW
Jason B O'Neal, Andrew D Shaw, Frederic T Billings
Acute kidney injury (AKI) complicates recovery from cardiac surgery in up to 30 % of patients, injures and impairs the function of the brain, lungs, and gut, and places patients at a 5-fold increased risk of death during hospitalization. Renal ischemia, reperfusion, inflammation, hemolysis, oxidative stress, cholesterol emboli, and toxins contribute to the development and progression of AKI. Preventive strategies are limited, but current evidence supports maintenance of renal perfusion and intravascular volume while avoiding venous congestion, administration of balanced salt as opposed to high-chloride intravenous fluids, and the avoidance or limitation of cardiopulmonary bypass exposure...
July 4, 2016: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/27324237/immunology-of-hepatic-diseases-during-pregnancy
#11
Lars Bremer, Christoph Schramm, Gisa Tiegs
The mother's immune system has to adapt to pregnancy accepting the semi-allograft fetus and preventing harmful effects to the developing child. Aberrations in feto-maternal immune adaptation may result in disease of the mother, such as liver injury. Five pregnancy-associated liver disorders have been described so far, however, little is known concerning immune alterations promoting the respective disease. These liver disorders are pre-eclampsia, hemolysis, elevated liver enzymes, low platelet count (HELLP), acute fatty liver, hyperemesis gravidarum, and intrahepatic cholestasis of pregnancy...
June 20, 2016: Seminars in Immunopathology
https://www.readbyqxmd.com/read/27290843/-frequency-and-maternal-complications-of-the-criteria-of-hemolysis-in-preeclamptic-patients-with-hellp-syndrome-treated-in-an-intensive-care-unit
#12
J G Vázquez-Rodríguez, C D Ríos-Gutiérrez, E P Paredes-Lozano, A García-Flores
BACKGROUND: HELLP syndrome is an aggressive form of preeclampsia related with hemolysis and its complications. OBJECTIVE: To determine the frequency of the appearance of criteria of hemolysis and maternal complications in preeclamptic patients with HELLP syndrome treated in an intensive care unit. MATERIAL AND METHODS: We carried out a cross-sectional study in 50 preeclamptic women with HELLP syndrome admitted to intensive care unit to determine the presence of the following criteria of hemolysis: peripheral blood schistocytes, anemia (hemoglobin ≤ 10 g/dL), lactate dehydrogenase ≥ 600 U/L, indirect bilirubin ≥ 0...
January 2016: Ginecología y Obstetricia de México
https://www.readbyqxmd.com/read/27247935/obstructive-form-of-hypertrophic-cardiomyopathy-left-ventricular-outflow-tract-gradient-novel-methods-of-provocation-monitoring-of-biomarkers-and-recent-advances-in-the-treatment
#13
REVIEW
Pawel Petkow Dimitrow, Renata Rajtar-Salwa
Dynamic (latent or/and labile) obstruction of left ventricular outflow (LVOT) was recognized from the earliest clinical descriptions of hypertrophic cardiomyopathy (HCM) and has proved to be a complex phenomenon, as well as arguably the most audible ("visible") pathophysiological hallmark of this heterogeneous disease. The aim of the current review is focused on two novel issues in a subgroup of obstructive HCM. Firstly, the important methodological problem in HCM is the examination of a subgroup of patients with nonobstructive hypertrophy in resting conditions and hard, but possible provoking obstruction...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27126442/neuroenzymatic-activity-and-physiological-energetics-in-manila-clam-ruditapes-philippinarum-during-short-term-sublethal-exposure-to-harmful-alga-heterocapsa-circularisquama
#14
Leila Basti, Satoshi Nagai, Satoshi Watanabe, Tatsuya Oda, Yuji Tanaka
The harmful alga, Heterocapsa circularisquama, causes recurrent mortalities of bivalve molluscs in Japan, with demonstrated hemolysis and cytotoxicity in rabbit erythrocytes, HeLa cells, and bivalve tissues. Nonetheless, the effects of exposure to sublethal cell densities on the physiological energetics of bivalves have not been investigated, nor the potential involvement of neurotoxicity. In the present study, two sets of experiments were conducted with adult clams, Ruditapes philippinarum. In the first set, the clearance rate (CR), respiration rate (RR), absorption efficiency (AE), ingestion rate (IR), and absorption rate (AR) were examined in clams exposed to H...
July 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27117632/erythrocyte-oxidative-stress-markers-in-children-with-sickle-cell-disease
#15
Priscila Bacarin Hermann, Mara Albonei Dudeque Pianovski, Railson Henneberg, Aguinaldo José Nascimento, Maria Suely Soares Leonart
OBJECTIVE: To determine eight parameters of oxidative stress markers in erythrocytes from children with sickle cell disease and compare with the same parameters in erythrocytes from healthy children, since oxidative stress plays an important role in the pathophysiology of sickle cell disease and because this disease is a serious public health problem in many countries. METHODS: Blood samples were obtained from 45 children with sickle cell disease (21 males and 24 females with a mean age of 9 years; range: 3-13 years) and 280 blood samples were obtained from children without hemoglobinopathies (137 males and 143 females with a mean age of 10 years; range: 8-11 years), as a control group...
July 2016: Jornal de Pediatria
https://www.readbyqxmd.com/read/27095294/donor-variation-effect-on-red-blood-cell-storage-lesion-a-close-relationship-emerges
#16
REVIEW
Vassilis L Tzounakas, Anastasios G Kriebardis, Issidora S Papassideri, Marianna H Antonelou
Although the molecular pathways leading to the progressive deterioration of stored red blood cells (RBC storage lesion) and the clinical relevance of storage-induced changes remain uncertain, substantial donor-specific variability in RBC performance during storage, and posttransfusion has been established ("donor-variation effect"). In-bag hemolysis and numerous properties of the RBC units that may affect transfusion efficacy have proved to be strongly donor-specific. Donor-variation effect may lead to the production of highly unequal blood labile products even when similar storage strategy and duration are applied...
August 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27054342/comparison-of-methodologies-to-detect-low-levels-of-hemolysis-in-serum-for-accurate-assessment-of-serum-micrornas
#17
COMPARATIVE STUDY
Jaynish S Shah, Patsy S Soon, Deborah J Marsh
microRNAs have emerged as powerful regulators of many biological processes, and their expression in many cancer tissues has been shown to correlate with clinical parameters such as cancer type and prognosis. Present in a variety of biological fluids, microRNAs have been described as a 'gold mine' of potential noninvasive biomarkers. Release of microRNA content of blood cells upon hemolysis dramatically alters the microRNA profile in blood, potentially affecting levels of a significant number of proposed biomarker microRNAs and, consequently, accuracy of serum or plasma-based tests...
2016: PloS One
https://www.readbyqxmd.com/read/27028307/donor-variation-effect-on-red-blood-cell-storage-lesion-a-multivariable-yet-consistent-story
#18
Vassilis L Tzounakas, Hara T Georgatzakou, Anastasios G Kriebardis, Artemis I Voulgaridou, Konstantinos E Stamoulis, Leontini E Foudoulaki-Paparizos, Marianna H Antonelou, Issidora S Papassideri
BACKGROUND: Previous studies have shown that baseline hematologic characteristics concerning or influencing red blood cell (RBC) properties might affect storage lesion development in individual donors. This study was conducted to evaluate whether variation in hemolysis, microparticle accumulation, phosphatidylserine (PS) exposure, and other storage lesion-associated variables might be a function of the prestorage hematologic and biologic profiles of the donor. STUDY DESIGN AND METHODS: Ten eligible, regular blood donors were paired and studied before donation (fresh blood) and during storage of RBCs in standard blood banking conditions...
March 29, 2016: Transfusion
https://www.readbyqxmd.com/read/26961071/inheritance-of-the-bantu-benin-haplotype-causes-less-severe-hemolytic-and-oxidative-stress-in-sickle-cell-anemia-patients-treated-with-hydroxycarbamide
#19
Jéssika V Okumura, Danilo G H Silva, Lidiane S Torres, Edis Belini-Junior, Willian M Barberino, Renan G Oliveira, Gisele C S Carrocini, Gabriela B Gelaleti, Clarisse L C Lobo, Claudia R Bonini-Domingos
Beta S-globin gene cluster haplotypes (β(S)-haplotypes) can modulate the response to hydroxycarbamide (HC) treatment in sickle cell anemia (SCA) patients. In Brazil, the most common haplotypes are Bantu and Benin, and both confer a poor prognosis for patients when untreated with HC. We evaluated oxidative and hemolytic biomarkers in 48 SCA patients undergoing HC treatment separated in three subgroups: Bantu/Bantu, Bantu/Benin and Benin/Benin haplotype. On the basis of reduced haptoglobin (HP) levels, patients with Bantu/Bantu haplotypes had 3...
July 2016: Journal of Human Genetics
https://www.readbyqxmd.com/read/26952641/circulating-mirnas-as-biomarkers-of-acute-muscle-damage-in-rats
#20
Julien Siracusa, Nathalie Koulmann, Stéphanie Bourdon, Marie-Emmanuelle Goriot, Sébastien Banzet
Skeletal muscle damage is an often-occurring event. Diagnosis using the classic blood marker creatine kinase sometimes yields unsatisfactory results due to great interindividual variability. Therefore, the identification of reliable biomarkers is important. Our aim was to detect and characterize circulating miRNAs in plasma in response to acute notexin-induced muscle damage in rats. Real-time quantitative RT-PCR profiling led to the identification of miRNAs that were highly increased in plasma in response to notexin injection into several muscles, namely miR-1-3p, -133a-3p, -133b-3p, -206-3p, -208b-3p, and -499-5p, as well as miR-378a-3p and miR-434-3p...
May 2016: American Journal of Pathology
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